MD13G1026800.v1.1

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003528
Physical Location & Seq
Reverse (-)
1948817 .. 1950166
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1026800.v1.1.491

Sequence Viewer

Length: 441 bp
ATGGGTGTTCTGGATGCTGTTTCGGACTATTTCACAATCTCTAAACCAAGAAAGAAACGAAAGCCAATGCAGACGGTTGAAATCAAGGTGAAAATGGACTGTGATGGCTGTGAAAGAAGAGTCAAAAATGCTGTTTCTCACATGAAAGGGGCTAAGCAAGTGGAGGTGGACAGAAAGCAGAGCAGGGTGACGGTGACGGGATACGTGGAGCCAAACAAGGTGTTAAAGAGAGTGAAGAGAACTGGGAAGAAAGCAGAGATTTGGCCGTATGTGCCTTACAACTTAGTAGCTTATCCCTATGTCGCCGGAGCTTATGACAAGAAGGCTCCCTCCGGCTACGTGAGGAACGTGGCTCAGGCACTTCCGCCCCCGGATGCGCCGGACGAAAAGTACACCTCTATGTTTAGCGACGAAAATCCACATGCTTGTTCTATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

147

Amino Acids

16.48

Weight (kDa)

9.59

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 29 - 84 1.9e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 365
AcoI YGGCCR 1 cut(s) 263
AfaI GTAC 1 cut(s) 392
AgsI TTSAA 1 cut(s) 80
AluBI AGCT 2 cut(s) 290, 311
AluI AGCT 2 cut(s) 290, 311
AoxI GGCC 1 cut(s) 263
AspLEI GCGC 1 cut(s) 379
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 3 cut(s) 100, 199, 205
BccI CCATC 1 cut(s) 98
BceAI ACGGC 1 cut(s) 250
BciVI GTATCC 1 cut(s) 194
BcnI CCSGG 1 cut(s) 371
BfuI GTATCC 1 cut(s) 194
BlpI GCTNAGC 1 cut(s) 153
Bme1390I CCNGG 1 cut(s) 371
BmiI GGNNCC 2 cut(s) 210, 327
BmrFI CCNGG 1 cut(s) 371
BmrI ACTGGG 1 cut(s) 252
BmsI GCATC 2 cut(s) 4, 364
BmuI ACTGGG 1 cut(s) 252
Bpu10I CCTNAGC 1 cut(s) 354
Bpu1102I GCTNAGC 1 cut(s) 153
BpuMI CCSGG 1 cut(s) 371
BsaAI YACGTR 2 cut(s) 205, 340
BsaJI CCNNGG 1 cut(s) 369
Bse1I ACTGG 1 cut(s) 247
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 2 cut(s) 19, 379
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 247
BshFI GGCC 1 cut(s) 265
BsiSI CCGG 4 cut(s) 306, 333, 371, 380
BsnI GGCC 1 cut(s) 265
Bsp1720I GCTNAGC 1 cut(s) 153
BspACI CCGC 1 cut(s) 365
BspANI GGCC 1 cut(s) 265
BspCNI CTCAG 1 cut(s) 367
BspLI GGNNCC 2 cut(s) 210, 327
BsrI ACTGG 1 cut(s) 247
BssECI CCNNGG 1 cut(s) 369
Bst4CI ACNGT 3 cut(s) 76, 101, 193
Bst6I CTCTTC 2 cut(s) 112, 230
BstBAI YACGTR 2 cut(s) 205, 340
BstDEI CTNAG 3 cut(s) 153, 283, 354
BstF5I GGATG 2 cut(s) 19, 379
BstHHI GCGC 1 cut(s) 379
BstMWI GCNNNNNNNGC 1 cut(s) 271
BstNSI RCATGY 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 369
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 1 cut(s) 265
BtsCI GGATG 2 cut(s) 19, 379
CfoI GCGC 1 cut(s) 379
Csp6I GTAC 1 cut(s) 391
CviAII CATG 3 cut(s) 142, 422, 436
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 3 cut(s) 153, 283, 354
EaeI YGGCCR 1 cut(s) 263
Eam1104I CTCTTC 2 cut(s) 112, 230
EarI CTCTTC 2 cut(s) 112, 230
EciI GGCGGA 1 cut(s) 354
FaeI CATG 3 cut(s) 145, 425, 439
FaiI YATR 7 cut(s) 143, 270, 300, 315, 401, 423, 437
FatI CATG 3 cut(s) 141, 421, 435
FokI GGATG 2 cut(s) 26, 386
GlaI GCGC 1 cut(s) 378
HaeIII GGCC 1 cut(s) 265
HapII CCGG 4 cut(s) 306, 333, 371, 380
HhaI GCGC 1 cut(s) 379
Hin1II CATG 3 cut(s) 145, 425, 439
Hin6I GCGC 1 cut(s) 377
HinP1I GCGC 1 cut(s) 377
HinfI GANTC 1 cut(s) 120
HpaII CCGG 4 cut(s) 306, 333, 371, 380
HphI GGTGA 3 cut(s) 100, 199, 205
Hpy166II GTNNAC 2 cut(s) 169, 393
Hpy188I TCNGA 1 cut(s) 25
Hpy188III TCNNGA 1 cut(s) 11
Hpy8I GTNNAC 2 cut(s) 169, 393
Hpy99I CGWCG 1 cut(s) 413
HpyAV CCTTC 1 cut(s) 316
HpyCH4III ACNGT 3 cut(s) 76, 101, 193
HpyCH4IV ACGT 3 cut(s) 204, 339, 348
HpyCH4V TGCA 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 1 cut(s) 271
HpyF3I CTNAG 3 cut(s) 153, 283, 354
HpySE526I ACGT 3 cut(s) 204, 339, 348
Hsp92II CATG 3 cut(s) 145, 425, 439
HspAI GCGC 1 cut(s) 377
LmnI GCTCC 3 cut(s) 208, 308, 331
LpnPI CCDG 7 cut(s) 169, 228, 319, 341, 346, 384, 393
LweI GCATC 2 cut(s) 4, 364
MaeII ACGT 3 cut(s) 204, 339, 348
MaeIII GTNAC 2 cut(s) 187, 193
MboII GAAGA 3 cut(s) 129, 247, 259
MlyI GAGTC 1 cut(s) 129
MnlI CCTC 4 cut(s) 157, 336, 340, 406
MseI TTAA 1 cut(s) 224
MslI CAYNNNNRTG 1 cut(s) 398
MspI CCGG 4 cut(s) 306, 333, 371, 380
MspR9I CCNGG 1 cut(s) 371
MwoI GCNNNNNNNGC 1 cut(s) 271
NciI CCSGG 1 cut(s) 371
NlaIII CATG 3 cut(s) 145, 425, 439
NlaIV GGNNCC 2 cut(s) 210, 327
NmuCI GTSAC 2 cut(s) 187, 193
NspI RCATGY 1 cut(s) 425
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
Ppu21I YACGTR 2 cut(s) 205, 340
PspN4I GGNNCC 2 cut(s) 210, 327
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
RseI CAYNNNNRTG 1 cut(s) 398
SaqAI TTAA 1 cut(s) 224
SchI GAGTC 1 cut(s) 129
ScrFI CCNGG 1 cut(s) 371
SetI ASST 9 cut(s) 90, 168, 207, 222, 292, 313, 342, 351, 398
SfaNI GCATC 2 cut(s) 4, 364
SmiMI CAYNNNNRTG 1 cut(s) 398
SsiI CCGC 1 cut(s) 365
StyD4I CCNGG 1 cut(s) 369
TaaI ACNGT 3 cut(s) 76, 101, 193
TaiI ACGT 3 cut(s) 207, 342, 351
TatI WGTACW 1 cut(s) 390
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TseFI GTSAC 2 cut(s) 187, 193
Tsp45I GTSAC 2 cut(s) 187, 193
TspDTI ATGAA 1 cut(s) 158
XceI RCATGY 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.