FvH4_4g33400

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008919
Physical Location & Seq
Forward (+)
31636002 .. 31638415
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33400.t1

Sequence Viewer

Length: 1128 bp
ATGGCAAAGTCAGTTCCAATGAATGGGAAACATTTCACGAAATCGATAACAGGTTCAATGCCAATGAACGGTGGAAGTGGTACTTACAGCTACTCCAAGAACTCCTACTTCCAGAGAAAAGCTGCAGAGATTGAAAGGGAGAAGATCATGGAGGTGATTGCTGAGAAGCTTGATATGAAGAACCTCTCCTCAGTTTCAAACACCATTCGCGTTGCAGACTTGGGATGTTCAACCGGACCAAACACTTTCGTGACAATGCAAAACATACTAGAAGCCATGCAACAAAAGTACCGATCCCAATGTAATGCTCATGATGATCATCAAACAATGCCTGAATTTCAAGTGTTCTTCAACGACCTAGTGTTGAATGACTTCAATACCTTGTTTACCTCTCTTCCCAAAGACAAACAATACTTTGCAGCAGGCGTACCAGGGTCTTTCCACGGCCGTCTGTTTCCGGAGTCCTCTATCCACCTTGTGCACACCTCACATTCCCTCCACTGGCTCTCTGAGTTGCCAGAAGAGTTGCAAGACAAGGACTCTCCAGCATGGAACAAAGGGAGGATTCACTACACAAGTGCCCCTAATGAAGTGGTTGATGCTTACATGTCCCAGTTTGCTAAGGAAATGGACAACTTTCTCGAAGCCAGGGCTAAAGAACTTGTGCCTGGAGGAATGATGGTGATGATCTTGACTGGAACTATTAAAGGCAGGCCTCATTCGGAAACCGCACCTGGCATGATGTACAATGCAATATCATCCAGCCTCATGGATATGGAAAAAGAGGGATTGATTGATGAAGGTCAAGTGGATACGTTCAACCTGCCTCTCTACGCTGCTTCACTAGAGGAGTTGGAGGGGCTTGTGGTTAAAAATGGGTGCTTTAGCATCGAGAAGATGGAATTTTCAGACCCAGCAACATGGTTGAAAGGTCCGCTTGACATAACGCAATGGGTGATGCATATACGAGCTGTAATGGAATGCATGTTCGCCACACACTTTGGAAGTCATGAGATTGTAGATCAAATGTTTCAACGTTCAACTCAAAAACTTTCACACCAGTCCGAGCTCATAAACTCAAGGTGCCACGAGCCAAGTCAGCTGCTTGCTGTTCTCAAGCGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

376

Amino Acids

42.27

Weight (kDa)

5.84

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 62 - 374 2.4e-106 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 831
AccB1I GGYRCC 1 cut(s) 1083
AccB7I CCANNNNNTGG 1 cut(s) 23
AccII CGCG 1 cut(s) 210
AccIII TCCGGA 1 cut(s) 457
AciI CCGC 2 cut(s) 729, 935
AclI AACGTT 1 cut(s) 1036
AclWI GGATC 1 cut(s) 288
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 2 cut(s) 335, 902
AdeI CACNNNGTG 1 cut(s) 478
AfaI GTAC 4 cut(s) 82, 290, 429, 746
AfiI CCNNNNNNNGG 3 cut(s) 23, 68, 501
AflIII ACRYGT 1 cut(s) 606
AjnI CCWGG 4 cut(s) 430, 647, 667, 733
AleI CACNNNNGTG 1 cut(s) 248
AloI GAACNNNNNNTCC 2 cut(s) 971, 1003
AluBI AGCT 6 cut(s) 90, 122, 169, 971, 1069, 1102
AluI AGCT 6 cut(s) 90, 122, 169, 971, 1069, 1102
Alw21I GWGCWC 2 cut(s) 483, 1071
Alw44I GTGCAC 1 cut(s) 479
AlwI GGATC 1 cut(s) 288
Aor13HI TCCGGA 1 cut(s) 457
AoxI GGCC 2 cut(s) 445, 713
ApaLI GTGCAC 1 cut(s) 479
ApeKI GCWGC 4 cut(s) 122, 419, 836, 1102
ApoI RAATTY 2 cut(s) 335, 902
Asp700I GAANNNNTTC 2 cut(s) 32, 371
AspS9I GGNCC 2 cut(s) 236, 932
AsuHPI GGTGA 3 cut(s) 166, 694, 967
AvaII GGWCC 2 cut(s) 236, 932
BaeGI GKGCMC 2 cut(s) 483, 583
BaeI ACNNNNGTAYC 2 cut(s) 272, 305
BanI GGYRCC 1 cut(s) 1083
BanII GRGCYC 1 cut(s) 1071
BauI CACGAG 1 cut(s) 1088
Bbv12I GWGCWC 2 cut(s) 483, 1071
BbvI GCAGC 4 cut(s) 109, 431, 823, 1089
BccI CCATC 2 cut(s) 673, 892
BceAI ACGGC 2 cut(s) 432, 460
BciT130I CCWGG 4 cut(s) 432, 649, 669, 735
BciVI GTATCC 1 cut(s) 805
BclI TGATCA 1 cut(s) 316
BfaI CTAG 3 cut(s) 269, 359, 845
BfmI CTRYAG 1 cut(s) 123
BfuAI ACCTGC 1 cut(s) 831
BfuI GTATCC 1 cut(s) 805
BisI GCNGC 4 cut(s) 123, 420, 837, 1103
BlsI GCNGC 4 cut(s) 124, 421, 838, 1104
Bme1390I CCNGG 4 cut(s) 432, 649, 669, 735
Bme18I GGWCC 2 cut(s) 236, 932
BmgT120I GGNCC 2 cut(s) 236, 932
BmiI GGNNCC 1 cut(s) 1085
BmrFI CCNGG 4 cut(s) 432, 649, 669, 735
BmrI ACTGGG 1 cut(s) 607
BmsI GCATC 3 cut(s) 589, 897, 948
BmuI ACTGGG 1 cut(s) 607
BpmI CTGGAG 2 cut(s) 528, 690
Bpu10I CCTNAGC 1 cut(s) 621
BpuEI CTTGAG 2 cut(s) 1063, 1100
Bsa29I ATCGAT 1 cut(s) 44
BsaBI GATNNNNATC 1 cut(s) 318
BsaJI CCNNGG 3 cut(s) 431, 442, 648
BsaWI WCCGGW 2 cut(s) 233, 457
BsaXI ACNNNNNCTCC 4 cut(s) 77, 107, 480, 510
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 68, 501
Bse1I ACTGG 4 cut(s) 506, 613, 700, 1060
Bse3DI GCAATG 1 cut(s) 956
Bse8I GATNNNNATC 1 cut(s) 318
BseAI TCCGGA 1 cut(s) 457
BseBI CCWGG 4 cut(s) 432, 649, 669, 735
BseCI ATCGAT 1 cut(s) 44
BseDI CCNNGG 3 cut(s) 431, 442, 648
BseGI GGATG 2 cut(s) 230, 758
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 3 cut(s) 23, 68, 501
BseMI GCAATG 1 cut(s) 956
BseMII CTCAG 3 cut(s) 153, 204, 501
BseNI ACTGG 4 cut(s) 506, 613, 700, 1060
BseRI GAGGAG 2 cut(s) 178, 863
BseSI GKGCMC 2 cut(s) 483, 583
BseX3I CGGCCG 1 cut(s) 445
BseXI GCAGC 4 cut(s) 109, 431, 823, 1089
BseYI CCCAGC 1 cut(s) 913
Bsh1236I CGCG 1 cut(s) 210
Bsh1285I CGRYCG 1 cut(s) 448
BshFI GGCC 2 cut(s) 447, 715
BshNI GGYRCC 1 cut(s) 1083
BshVI ATCGAT 1 cut(s) 44
BsiEI CGRYCG 1 cut(s) 448
BsiHKAI GWGCWC 2 cut(s) 483, 1071
BsiSI CCGG 2 cut(s) 234, 458
BslFI GGGAC 1 cut(s) 595
BslI CCNNNNNNNGG 3 cut(s) 23, 68, 501
BsmFI GGGAC 1 cut(s) 595
BsmI GAATGC 1 cut(s) 986
BsnI GGCC 2 cut(s) 447, 715
Bsp1286I GDGCHC 3 cut(s) 483, 583, 1071
Bsp13I TCCGGA 1 cut(s) 457
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 5 cut(s) 144, 293, 316, 687, 1021
BspACI CCGC 2 cut(s) 729, 935
BspANI GGCC 2 cut(s) 447, 715
BspCNI CTCAG 3 cut(s) 154, 203, 502
BspDI ATCGAT 1 cut(s) 44
BspEI TCCGGA 1 cut(s) 457
BspFNI CGCG 1 cut(s) 210
BspHI TCATGA 2 cut(s) 310, 1009
BspLI GGNNCC 1 cut(s) 1085
BspMAI CTGCAG 1 cut(s) 127
BspMI ACCTGC 1 cut(s) 831
BspPI GGATC 1 cut(s) 288
BspT107I GGYRCC 1 cut(s) 1083
BsrDI GCAATG 1 cut(s) 956
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 4 cut(s) 506, 613, 700, 1060
BssECI CCNNGG 3 cut(s) 431, 442, 648
BssMI GATC 5 cut(s) 144, 293, 316, 687, 1021
BssSI CACGAG 1 cut(s) 1088
Bst2BI CACGAG 1 cut(s) 1088
Bst2UI CCWGG 4 cut(s) 432, 649, 669, 735
Bst4CI ACNGT 1 cut(s) 71
Bst6I CTCTTC 2 cut(s) 399, 516
BstAUI TGTACA 1 cut(s) 744
BstC8I GCNNGC 3 cut(s) 424, 713, 1107
BstDEI CTNAG 4 cut(s) 162, 190, 510, 621
BstDSI CCRYGG 1 cut(s) 442
BstF5I GGATG 2 cut(s) 230, 758
BstFNI CGCG 1 cut(s) 210
BstKTI GATC 5 cut(s) 147, 296, 319, 690, 1024
BstMBI GATC 5 cut(s) 144, 293, 316, 687, 1021
BstMCI CGRYCG 1 cut(s) 448
BstMWI GCNNNNNNNGC 1 cut(s) 1099
BstNI CCWGG 4 cut(s) 432, 649, 669, 735
BstNSI RCATGY 2 cut(s) 610, 988
BstSCI CCNGG 4 cut(s) 430, 647, 667, 733
BstSFI CTRYAG 1 cut(s) 123
BstSLI GKGCMC 2 cut(s) 483, 583
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 4 cut(s) 109, 431, 823, 1089
BstXI CCANNNNNNTGG 2 cut(s) 769, 921
BstZI CGGCCG 1 cut(s) 445
Bsu15I ATCGAT 1 cut(s) 44
BsuI GTATCC 1 cut(s) 805
BsuRI GGCC 2 cut(s) 447, 715
BsuTUI ATCGAT 1 cut(s) 44
BtgI CCRYGG 1 cut(s) 442
BtsCI GGATG 2 cut(s) 230, 758
BtsIMutI CAGTG 1 cut(s) 499
BveI ACCTGC 1 cut(s) 831
Cac8I GCNNGC 3 cut(s) 424, 713, 1107
CciI TCATGA 2 cut(s) 310, 1009
Cfr13I GGNCC 2 cut(s) 236, 932
ClaI ATCGAT 1 cut(s) 44
Csp6I GTAC 4 cut(s) 81, 289, 428, 745
CspCI CAANNNNNGTGG 2 cut(s) 982, 1017
CviQI GTAC 4 cut(s) 81, 289, 428, 745
DdeI CTNAG 4 cut(s) 162, 190, 510, 621
DpnI GATC 5 cut(s) 146, 295, 318, 689, 1023
DpnII GATC 5 cut(s) 144, 293, 316, 687, 1021
DraIII CACNNNGTG 1 cut(s) 478
EaeI YGGCCR 1 cut(s) 445
EagI CGGCCG 1 cut(s) 445
Eam1104I CTCTTC 2 cut(s) 399, 516
EarI CTCTTC 2 cut(s) 399, 516
Ecl136II GAGCTC 1 cut(s) 1069
EclXI CGGCCG 1 cut(s) 445
Eco147I AGGCCT 1 cut(s) 715
Eco24I GRGCYC 1 cut(s) 1071
Eco47I GGWCC 2 cut(s) 236, 932
Eco52I CGGCCG 1 cut(s) 445
Eco53kI GAGCTC 1 cut(s) 1069
EcoICRI GAGCTC 1 cut(s) 1069
EcoRII CCWGG 4 cut(s) 430, 647, 667, 733
EcoT22I ATGCAT 2 cut(s) 963, 986
EcoT38I GRGCYC 1 cut(s) 1071
FalI AAGNNNNNCTT 4 cut(s) 67, 99, 921, 953
FaqI GGGAC 1 cut(s) 595
FbaI TGATCA 1 cut(s) 316
Fnu4HI GCNGC 4 cut(s) 123, 420, 837, 1103
FokI GGATG 2 cut(s) 237, 745
FriOI GRGCYC 1 cut(s) 1071
Fsp4HI GCNGC 4 cut(s) 123, 420, 837, 1103
FspBI CTAG 3 cut(s) 269, 359, 845
GluI GCNGC 4 cut(s) 123, 420, 837, 1103
GsaI CCCAGC 1 cut(s) 917
GsuI CTGGAG 2 cut(s) 528, 690
HaeIII GGCC 2 cut(s) 447, 715
HapII CCGG 2 cut(s) 234, 458
HindIII AAGCTT 1 cut(s) 167
HinfI GANTC 3 cut(s) 461, 539, 565
HpaII CCGG 2 cut(s) 234, 458
HphI GGTGA 3 cut(s) 166, 694, 967
Hpy166II GTNNAC 2 cut(s) 387, 481
Hpy188I TCNGA 4 cut(s) 511, 724, 910, 1066
Hpy188III TCNNGA 9 cut(s) 37, 112, 250, 311, 458, 641, 691, 892, 1010
Hpy8I GTNNAC 2 cut(s) 387, 481
HpyAV CCTTC 1 cut(s) 794
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4IV ACGT 2 cut(s) 815, 1036
HpyF10VI GCNNNNNNNGC 1 cut(s) 1099
HpyF3I CTNAG 4 cut(s) 162, 190, 510, 621
HpySE526I ACGT 2 cut(s) 815, 1036
Kpn2I TCCGGA 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 316
Kzo9I GATC 5 cut(s) 144, 293, 316, 687, 1021
Lsp1109I GCAGC 4 cut(s) 109, 431, 823, 1089
LweI GCATC 3 cut(s) 589, 897, 948
MaeI CTAG 3 cut(s) 269, 359, 845
MaeII ACGT 2 cut(s) 815, 1036
MaeIII GTNAC 1 cut(s) 250
MalI GATC 5 cut(s) 146, 295, 318, 689, 1023
MboI GATC 5 cut(s) 144, 293, 316, 687, 1021
MboII GAAGA 6 cut(s) 154, 190, 340, 386, 533, 907
MhlI GDGCHC 3 cut(s) 483, 583, 1071
MluCI AATT 2 cut(s) 335, 902
MlyI GAGTC 2 cut(s) 470, 533
MmeI TCCRAC 1 cut(s) 834
Mph1103I ATGCAT 2 cut(s) 963, 986
MroI TCCGGA 1 cut(s) 457
MroXI GAANNNNTTC 2 cut(s) 32, 371
MseI TTAA 2 cut(s) 705, 870
MslI CAYNNNNRTG 3 cut(s) 152, 248, 773
MspA1I CMGCKG 1 cut(s) 1102
MspI CCGG 2 cut(s) 234, 458
MspR9I CCNGG 4 cut(s) 432, 649, 669, 735
Mva1269I GAATGC 1 cut(s) 986
MvaI CCWGG 4 cut(s) 432, 649, 669, 735
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 1099
NdeII GATC 5 cut(s) 144, 293, 316, 687, 1021
NlaIV GGNNCC 1 cut(s) 1085
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 2 cut(s) 963, 986
NspI RCATGY 2 cut(s) 610, 988
OliI CACNNNNGTG 1 cut(s) 248
PagI TCATGA 2 cut(s) 310, 1009
PceI AGGCCT 1 cut(s) 715
PciI ACATGT 1 cut(s) 606
PctI GAATGC 1 cut(s) 986
PdmI GAANNNNTTC 2 cut(s) 32, 371
PfeI GAWTC 1 cut(s) 565
PflMI CCANNNNNTGG 1 cut(s) 23
PkrI GCNGC 4 cut(s) 124, 421, 838, 1104
PleI GAGTC 2 cut(s) 469, 533
PpsI GAGTC 2 cut(s) 469, 533
PscI ACATGT 1 cut(s) 606
Psp124BI GAGCTC 1 cut(s) 1071
Psp1406I AACGTT 1 cut(s) 1036
Psp6I CCWGG 4 cut(s) 430, 647, 667, 733
PspFI CCCAGC 1 cut(s) 913
PspGI CCWGG 4 cut(s) 430, 647, 667, 733
PspN4I GGNNCC 1 cut(s) 1085
PspPI GGNCC 2 cut(s) 236, 932
PstI CTGCAG 1 cut(s) 127
PvuII CAGCTG 1 cut(s) 1102
RsaI GTAC 4 cut(s) 82, 290, 429, 746
RsaNI GTAC 4 cut(s) 81, 289, 428, 745
RseI CAYNNNNRTG 3 cut(s) 152, 248, 773
SacI GAGCTC 1 cut(s) 1071
SaqAI TTAA 2 cut(s) 705, 870
SatI GCNGC 4 cut(s) 123, 420, 837, 1103
Sau3AI GATC 5 cut(s) 144, 293, 316, 687, 1021
Sau96I GGNCC 2 cut(s) 236, 932
SchI GAGTC 2 cut(s) 470, 533
ScrFI CCNGG 4 cut(s) 432, 649, 669, 735
SduI GDGCHC 3 cut(s) 483, 583, 1071
SfaNI GCATC 3 cut(s) 589, 897, 948
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 2 cut(s) 236, 932
SmiMI CAYNNNNRTG 3 cut(s) 152, 248, 773
SmlI CTYRAG 2 cut(s) 1078, 1115
SmoI CTYRAG 2 cut(s) 1078, 1115
Sse9I AATT 2 cut(s) 335, 902
SseBI AGGCCT 1 cut(s) 715
SsiI CCGC 2 cut(s) 729, 935
SspMI CTAG 3 cut(s) 269, 359, 845
SstI GAGCTC 1 cut(s) 1071
StuI AGGCCT 1 cut(s) 715
StyD4I CCNGG 4 cut(s) 430, 647, 667, 733
TaaI ACNGT 1 cut(s) 71
TaiI ACGT 2 cut(s) 818, 1039
TaqI TCGA 3 cut(s) 44, 642, 891
TasI AATT 2 cut(s) 335, 902
TatI WGTACW 1 cut(s) 744
TfiI GAWTC 1 cut(s) 565
Tru1I TTAA 2 cut(s) 705, 870
Tru9I TTAA 2 cut(s) 705, 870
TscAI CASTG 1 cut(s) 506
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 4 cut(s) 122, 419, 836, 1102
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 5 cut(s) 35, 80, 191, 603, 813
TspRI CASTG 1 cut(s) 506
Van91I CCANNNNNTGG 1 cut(s) 23
VneI GTGCAC 1 cut(s) 479
VpaK11BI GGWCC 2 cut(s) 236, 932
XapI RAATTY 2 cut(s) 335, 902
XceI RCATGY 2 cut(s) 610, 988
XmnI GAANNNNTTC 2 cut(s) 32, 371
XspI CTAG 3 cut(s) 269, 359, 845
Zsp2I ATGCAT 2 cut(s) 963, 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.