MD16G1017100.v1.1

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008919
Physical Location & Seq
Forward (+)
1268489 .. 1269907
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1017100.v1.1.491

Sequence Viewer

Length: 609 bp
ATGCAATACTTTGCAGCCGGCGTGCCCGGTTCTTTCCATCTCCGTTTGTTTCCCGACTCGTCTATTCACTTTGTACATTCTTCATTATGTCTCCACCGGATCTCCAAGTCGCCGGAAGTATTGCAAAACAAGGCCTCTCCGGCCTGGAACAAGGGCAGGATTCACTACACAAGTGCACCGGATGAAGTAGTTGAGGTTTATGCGTCGCAGTTTGCCGAGGACATGGAGAATTTTCTGAATGCTAGGGCCAAAGAGCTTGTGCCTAAGGGAATGATGGTATTGATCTTGACTGCAACACCCAAAGGGATGCCTTATTCAGAACTTCCAACGGGTATGTGGTACGATTCTTTATCGTCTGTCCTCATGGATATGGCGAAAGAGGAAATAATTGAAGAAAGTAAAGTGGATTCCTTCAACTTGCCATATTATGCAGCCTCTGTAGAGGAGATGGAGGAGATAGTGGAGAAAAACGGGTGTTTCAGGATCCAAAGAATGGAGTCGTCAAACCAATCAGCATGGATGAAGGGTCCAGTTGACATTCCAGTTTGGGTGAGGCATATGAGAGCTGCAATGGAGGGAATGACACTTTGGAAGTGGGGTTATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

203

Amino Acids

22.98

Weight (kDa)

5.53

Isoelectric Point (pI)

56.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 2 - 194 1.2e-65 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 493
AclWI GGATC 3 cut(s) 107, 478, 491
AcsI RAATTY 1 cut(s) 229
AfaI GTAC 2 cut(s) 75, 341
AfiI CCNNNNNNNGG 1 cut(s) 493
AgsI TTSAA 2 cut(s) 392, 415
AjnI CCWGG 1 cut(s) 143
AjuI GAANNNNNNNTTGG 2 cut(s) 571, 603
AluBI AGCT 2 cut(s) 256, 566
AluI AGCT 2 cut(s) 256, 566
Alw21I GWGCWC 1 cut(s) 178
Alw26I GTCTC 1 cut(s) 95
Alw44I GTGCAC 1 cut(s) 174
AlwI GGATC 3 cut(s) 107, 478, 491
AlwNI CAGNNNCTG 1 cut(s) 437
AoxI GGCC 3 cut(s) 132, 141, 246
ApaLI GTGCAC 1 cut(s) 174
ApeKI GCWGC 3 cut(s) 14, 431, 566
ApoI RAATTY 1 cut(s) 229
AspS9I GGNCC 2 cut(s) 246, 527
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 1 cut(s) 562
AvaII GGWCC 1 cut(s) 527
AxyI CCTNAGG 1 cut(s) 264
BaeGI GKGCMC 2 cut(s) 27, 178
BamHI GGATCC 1 cut(s) 483
Bbv12I GWGCWC 1 cut(s) 178
BbvI GCAGC 3 cut(s) 26, 443, 553
BccI CCATC 3 cut(s) 45, 268, 442
BciT130I CCWGG 1 cut(s) 145
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 1 cut(s) 243
BfmI CTRYAG 1 cut(s) 438
BglI GCCNNNNNGGC 1 cut(s) 140
BisI GCNGC 3 cut(s) 15, 432, 567
BlsI GCNGC 3 cut(s) 16, 433, 568
Bme1390I CCNGG 2 cut(s) 27, 145
Bme18I GGWCC 1 cut(s) 527
BmgT120I GGNCC 2 cut(s) 246, 527
BmiI GGNNCC 2 cut(s) 485, 528
BmrFI CCNGG 2 cut(s) 27, 145
BmsI GCATC 1 cut(s) 297
BpuMI CCSGG 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 216
BsaWI WCCGGW 2 cut(s) 96, 178
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 1 cut(s) 493
Bse118I RCCGGY 1 cut(s) 17
Bse1I ACTGG 2 cut(s) 530, 542
Bse21I CCTNAGG 1 cut(s) 264
Bse3DI GCAATG 1 cut(s) 576
BseBI CCWGG 1 cut(s) 145
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 3 cut(s) 187, 312, 525
BseLI CCNNNNNNNGG 1 cut(s) 493
BseMI GCAATG 1 cut(s) 576
BseNI ACTGG 2 cut(s) 530, 542
BseRI GAGGAG 2 cut(s) 458, 467
BseSI GKGCMC 2 cut(s) 27, 178
BseXI GCAGC 3 cut(s) 26, 443, 553
BshFI GGCC 3 cut(s) 134, 143, 248
BsiHKAI GWGCWC 1 cut(s) 178
BsiSI CCGG 6 cut(s) 18, 27, 97, 113, 140, 179
BslI CCNNNNNNNGG 1 cut(s) 493
BsmAI GTCTC 1 cut(s) 95
BsmI GAATGC 1 cut(s) 244
BsnI GGCC 3 cut(s) 134, 143, 248
Bsp1286I GDGCHC 2 cut(s) 27, 178
Bsp1407I TGTACA 1 cut(s) 73
Bsp143I GATC 3 cut(s) 99, 282, 483
BspANI GGCC 3 cut(s) 134, 143, 248
BspLI GGNNCC 2 cut(s) 485, 528
BspPI GGATC 3 cut(s) 107, 478, 491
BsrDI GCAATG 1 cut(s) 576
BsrFI RCCGGY 1 cut(s) 17
BsrGI TGTACA 1 cut(s) 73
BsrI ACTGG 2 cut(s) 530, 542
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 3 cut(s) 99, 282, 483
Bst2UI CCWGG 1 cut(s) 145
BstAUI TGTACA 1 cut(s) 73
BstC8I GCNNGC 2 cut(s) 19, 23
BstDEI CTNAG 1 cut(s) 264
BstF5I GGATG 3 cut(s) 187, 312, 525
BstKTI GATC 3 cut(s) 102, 285, 486
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 3 cut(s) 99, 282, 483
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstNI CCWGG 1 cut(s) 145
BstSCI CCNGG 2 cut(s) 25, 143
BstSFI CTRYAG 1 cut(s) 438
BstSLI GKGCMC 2 cut(s) 27, 178
BstV1I GCAGC 3 cut(s) 26, 443, 553
BstX2I RGATCY 2 cut(s) 99, 483
BstYI RGATCY 2 cut(s) 99, 483
Bsu36I CCTNAGG 1 cut(s) 264
BsuRI GGCC 3 cut(s) 134, 143, 248
BtsCI GGATG 3 cut(s) 187, 312, 525
Cac8I GCNNGC 2 cut(s) 19, 23
CaiI CAGNNNCTG 1 cut(s) 437
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 2 cut(s) 246, 527
CseI GACGC 1 cut(s) 192
Csp6I GTAC 2 cut(s) 74, 340
CviAII CATG 3 cut(s) 223, 364, 516
CviJI RGCY 7 cut(s) 17, 134, 143, 248, 256, 434, 566
CviKI_1 RGCY 7 cut(s) 17, 134, 143, 248, 256, 434, 566
CviQI GTAC 2 cut(s) 74, 340
DdeI CTNAG 1 cut(s) 264
DpnI GATC 3 cut(s) 101, 284, 485
DpnII GATC 3 cut(s) 99, 282, 483
Eco147I AGGCCT 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 527
Eco81I CCTNAGG 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 143
FaeI CATG 3 cut(s) 226, 367, 519
FatI CATG 3 cut(s) 222, 363, 515
FauNDI CATATG 1 cut(s) 558
Fnu4HI GCNGC 3 cut(s) 15, 432, 567
FokI GGATG 3 cut(s) 194, 319, 532
Fsp4HI GCNGC 3 cut(s) 15, 432, 567
FspBI CTAG 1 cut(s) 243
GluI GCNGC 3 cut(s) 15, 432, 567
HaeIII GGCC 3 cut(s) 134, 143, 248
HapII CCGG 6 cut(s) 18, 27, 97, 113, 140, 179
HgaI GACGC 1 cut(s) 192
Hin1II CATG 3 cut(s) 226, 367, 519
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HinfI GANTC 5 cut(s) 56, 160, 344, 407, 497
HpaII CCGG 6 cut(s) 18, 27, 97, 113, 140, 179
HphI GGTGA 1 cut(s) 562
Hpy166II GTNNAC 2 cut(s) 176, 535
Hpy188I TCNGA 2 cut(s) 237, 319
Hpy188III TCNNGA 3 cut(s) 53, 286, 481
Hpy8I GTNNAC 2 cut(s) 176, 535
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 2 cut(s) 421, 517
HpyCH4V TGCA 7 cut(s) 4, 14, 124, 176, 293, 431, 569
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 1 cut(s) 264
Hsp92II CATG 3 cut(s) 226, 367, 519
KroI GCCGGC 1 cut(s) 17
KroNI GCCGGC 1 cut(s) 19
Kzo9I GATC 3 cut(s) 99, 282, 483
Lsp1109I GCAGC 3 cut(s) 26, 443, 553
LweI GCATC 1 cut(s) 297
MaeI CTAG 1 cut(s) 243
MalI GATC 3 cut(s) 101, 284, 485
MboI GATC 3 cut(s) 99, 282, 483
MboII GAAGA 2 cut(s) 72, 404
MflI RGATCY 2 cut(s) 99, 483
MhlI GDGCHC 2 cut(s) 27, 178
MluCI AATT 2 cut(s) 229, 387
MlyI GAGTC 2 cut(s) 50, 506
MmeI TCCRAC 1 cut(s) 350
MroNI GCCGGC 1 cut(s) 17
MslI CAYNNNNRTG 1 cut(s) 368
MspI CCGG 6 cut(s) 18, 27, 97, 113, 140, 179
MspR9I CCNGG 2 cut(s) 27, 145
Mva1269I GAATGC 1 cut(s) 244
MvaI CCWGG 1 cut(s) 145
MwoI GCNNNNNNNGC 1 cut(s) 140
NaeI GCCGGC 1 cut(s) 19
NciI CCSGG 1 cut(s) 27
NdeI CATATG 1 cut(s) 558
NdeII GATC 3 cut(s) 99, 282, 483
NgoMIV GCCGGC 1 cut(s) 17
NlaIII CATG 3 cut(s) 226, 367, 519
NlaIV GGNNCC 2 cut(s) 485, 528
NmeAIII GCCGAG 1 cut(s) 241
PceI AGGCCT 1 cut(s) 134
PctI GAATGC 1 cut(s) 244
PdiI GCCGGC 1 cut(s) 19
PfeI GAWTC 3 cut(s) 160, 344, 407
PflMI CCANNNNNTGG 1 cut(s) 493
PkrI GCNGC 3 cut(s) 16, 433, 568
PleI GAGTC 2 cut(s) 50, 505
PpsI GAGTC 2 cut(s) 50, 505
Psp6I CCWGG 1 cut(s) 143
PspGI CCWGG 1 cut(s) 143
PspN4I GGNNCC 2 cut(s) 485, 528
PspPI GGNCC 2 cut(s) 246, 527
PstNI CAGNNNCTG 1 cut(s) 437
PsuI RGATCY 2 cut(s) 99, 483
RsaI GTAC 2 cut(s) 75, 341
RsaNI GTAC 2 cut(s) 74, 340
RseI CAYNNNNRTG 1 cut(s) 368
SatI GCNGC 3 cut(s) 15, 432, 567
Sau3AI GATC 3 cut(s) 99, 282, 483
Sau96I GGNCC 2 cut(s) 246, 527
SchI GAGTC 2 cut(s) 50, 506
ScrFI CCNGG 2 cut(s) 27, 145
SduI GDGCHC 2 cut(s) 27, 178
SetI ASST 3 cut(s) 198, 258, 568
SfaNI GCATC 1 cut(s) 297
SfcI CTRYAG 1 cut(s) 438
SfiI GGCCNNNNNGGCC 1 cut(s) 140
SinI GGWCC 1 cut(s) 527
SmiMI CAYNNNNRTG 1 cut(s) 368
Sse9I AATT 2 cut(s) 229, 387
SseBI AGGCCT 1 cut(s) 134
SspMI CTAG 1 cut(s) 243
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 2 cut(s) 25, 143
TasI AATT 2 cut(s) 229, 387
TatI WGTACW 1 cut(s) 73
TfiI GAWTC 3 cut(s) 160, 344, 407
TseI GCWGC 3 cut(s) 14, 431, 566
TspDTI ATGAA 3 cut(s) 72, 198, 536
TspGWI ACGGA 1 cut(s) 32
Van91I CCANNNNNTGG 1 cut(s) 493
VneI GTGCAC 1 cut(s) 174
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 1 cut(s) 229
XcmI CCANNNNNNNNNTGG 1 cut(s) 333
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.