MD16G1016900.v1.1

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008919
Physical Location & Seq
Forward (+)
1261851 .. 1265760
3910 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1016900.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGCAGAAGGGGGCAAAGGAACGTTGCAGGTTTCAGCTGTTTCCATGAATCTCAACGGAGATCGTGAAAATAGTGCGATACCGATCACAACTTCTTTGCCTATGAACGGTGGAAAAGGCACTTACAGTTACTTCAACAACTCCTGCAGCCAGAGACAGTGCACTGAGGATGAGAAGAGAAAATTCATTGAGGAAATTGAGCAGAAGCTTGATATGAAAAAACTCTTCTCCCTTTCAAACACCATTCGTCTTGCGGACTTGGGATGTGCCACTGGACCAAACACTTTCATGATAATTCAAGACATACTAGAAGCCATGCAACGCAAACACCAATCCCAATTATCCCAATCATGTTTCAACGATGTTCAACATTCTGATGATCATCAAATGCCTGAATTTCAAGTCTTCTTTAACGACCAAGAGTCGAATGACTTCAACACCCTCTTCACCTCTCTCCCACAAGACAGGCAATACTTTGCAGCTGGCGTGCCCGGTTCTTTCCATCACCGTTTGTTTCCCGAGTCATCTATCCACTTTGTGCATACCTCATTATGTCTCCACTGGATCTCTAAGTCGCCGGAAGTATTGCAAAACAAGGCCTCTCCGACATGGAACAAGGGCAGGATTCACCACACAAGTGCACCGGATGAAGTAGTTGAGGCTTATGCGTCCCAGTTTGTGGAGGACATGGAGAATTTTTTGAATGCTAGGGCCAAAGAGCTTGTGCCTGAAGGAATGATGGTAATGATCTTGGTAGGAATTCCCAAAGGGACGCCTTATTCAGAAATTCCAATGGGTATGATGTGCAATTGTTTATCGTCTAGCCTCATGGATATGGCAAAAGAGGGAATAATTGAAGAAAGTAAAGTGGATTCCTTCAACTTGCCATTTTATGCAGCCTCTCTAGAGGAGATGGAGGAGATATTAGAGAAAAACGGGTGTTTTAAGATAGAGAGAATGGAGTCGACAAACCCAGCAGCATATCTGAAAGGTGGTCCAGTTGACATACCAGCTTGGGTGACGAATGTGAGGGCTGCAATGGAGGGAATGTTTGCCAAACACTTTGGAAGTGAGGTTATGGAAGAAATGTTTGGAAGATTGACCGATAAACTTGTAGACATTGCCGACCTCATAAACTCACGATGCGAAGTCAAAAGTCAGTTGCTTGCTGTTCTCAAGCGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

396

Amino Acids

44.38

Weight (kDa)

5.19

Isoelectric Point (pI)

45.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 71 - 394 2e-104 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 19
AccB7I CCANNNNNTGG 1 cut(s) 679
AccI GTMKAC 2 cut(s) 965, 1116
AciI CCGC 1 cut(s) 254
AclI AACGTT 1 cut(s) 23
AclWI GGATC 1 cut(s) 572
AcsI RAATTY 5 cut(s) 182, 395, 694, 759, 786
AcuI CTGAAG 1 cut(s) 750
AcyI GRCGYC 1 cut(s) 773
AdeI CACNNNGTG 1 cut(s) 538
AfiI CCNNNNNNNGG 2 cut(s) 107, 679
AhdI GACNNNNNGTC 1 cut(s) 421
AjuI GAANNNNNNNTTGG 2 cut(s) 1074, 1106
AleI CACNNNNGTG 1 cut(s) 636
AluBI AGCT 5 cut(s) 38, 208, 482, 721, 1013
AluI AGCT 5 cut(s) 38, 208, 482, 721, 1013
Alw21I GWGCWC 2 cut(s) 164, 643
Alw26I GTCTC 2 cut(s) 148, 560
Alw44I GTGCAC 2 cut(s) 160, 639
AlwI GGATC 1 cut(s) 572
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 2 cut(s) 597, 711
ApaLI GTGCAC 2 cut(s) 160, 639
ApeKI GCWGC 5 cut(s) 147, 479, 896, 977, 1034
ApoI RAATTY 5 cut(s) 182, 395, 694, 759, 786
Asp700I GAANNNNTTC 1 cut(s) 431
AspS9I GGNCC 3 cut(s) 275, 711, 995
AsuC2I CCSGG 1 cut(s) 492
AsuHPI GGTGA 4 cut(s) 439, 497, 620, 1030
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 2 cut(s) 275, 995
BaeGI GKGCMC 3 cut(s) 164, 492, 643
BbsI GAAGAC 1 cut(s) 397
Bbv12I GWGCWC 2 cut(s) 164, 643
BbvI GCAGC 5 cut(s) 159, 491, 908, 989, 1021
BccI CCATC 3 cut(s) 510, 733, 907
BclI TGATCA 1 cut(s) 379
BcnI CCSGG 1 cut(s) 492
BcoDI GTCTC 2 cut(s) 148, 560
BfaI CTAG 4 cut(s) 308, 708, 822, 905
BfmI CTRYAG 1 cut(s) 145
BfuAI ACCTGC 1 cut(s) 19
BisI GCNGC 5 cut(s) 148, 480, 897, 978, 1035
BlsI GCNGC 5 cut(s) 149, 481, 898, 979, 1036
Bme1390I CCNGG 1 cut(s) 492
Bme18I GGWCC 2 cut(s) 275, 995
BmeRI GACNNNNNGTC 1 cut(s) 421
BmeT110I CYCGRG 1 cut(s) 518
BmgT120I GGNCC 3 cut(s) 275, 711, 995
BmrFI CCNGG 1 cut(s) 492
BmrI ACTGGG 1 cut(s) 667
BmsI GCATC 1 cut(s) 1133
BmuI ACTGGG 1 cut(s) 667
BpiI GAAGAC 1 cut(s) 397
BpuEI CTTGAG 1 cut(s) 1160
BpuMI CCSGG 1 cut(s) 492
BsaBI GATNNNNATC 2 cut(s) 83, 381
BsaHI GRCGYC 1 cut(s) 773
BsaWI WCCGGW 1 cut(s) 643
BsaXI ACNNNNNCTCC 2 cut(s) 1034, 1064
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 679
Bse1I ACTGG 4 cut(s) 277, 566, 673, 998
Bse3DI GCAATG 2 cut(s) 1044, 1119
Bse8I GATNNNNATC 2 cut(s) 83, 381
BseGI GGATG 3 cut(s) 175, 269, 652
BseJI GATNNNNATC 2 cut(s) 83, 381
BseLI CCNNNNNNNGG 2 cut(s) 107, 679
BseMI GCAATG 2 cut(s) 1044, 1119
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 4 cut(s) 277, 566, 673, 998
BseRI GAGGAG 2 cut(s) 923, 932
BseSI GKGCMC 3 cut(s) 164, 492, 643
BseXI GCAGC 5 cut(s) 159, 491, 908, 989, 1021
BseYI CCCAGC 1 cut(s) 973
BshFI GGCC 2 cut(s) 599, 713
BsiHKAI GWGCWC 2 cut(s) 164, 643
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 3 cut(s) 492, 578, 644
BslFI GGGAC 2 cut(s) 655, 784
BslI CCNNNNNNNGG 2 cut(s) 107, 679
BsmAI GTCTC 2 cut(s) 148, 560
BsmFI GGGAC 2 cut(s) 655, 784
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 2 cut(s) 599, 713
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 3 cut(s) 164, 492, 643
Bsp143I GATC 5 cut(s) 61, 84, 379, 564, 747
BspACI CCGC 1 cut(s) 254
BspANI GGCC 2 cut(s) 599, 713
BspCNI CTCAG 1 cut(s) 157
BspHI TCATGA 1 cut(s) 288
BspMAI CTGCAG 1 cut(s) 149
BspMI ACCTGC 1 cut(s) 19
BspPI GGATC 1 cut(s) 572
BsrDI GCAATG 2 cut(s) 1044, 1119
BsrI ACTGG 4 cut(s) 277, 566, 673, 998
BssMI GATC 5 cut(s) 61, 84, 379, 564, 747
BssNI GRCGYC 1 cut(s) 773
Bst4CI ACNGT 4 cut(s) 110, 128, 159, 509
Bst6I CTCTTC 3 cut(s) 170, 230, 449
BstACI GRCGYC 1 cut(s) 773
BstC8I GCNNGC 3 cut(s) 484, 488, 1167
BstDEI CTNAG 2 cut(s) 165, 570
BstF5I GGATG 3 cut(s) 175, 269, 652
BstKTI GATC 5 cut(s) 64, 87, 382, 567, 750
BstMAI GTCTC 2 cut(s) 148, 560
BstMBI GATC 5 cut(s) 61, 84, 379, 564, 747
BstSCI CCNGG 1 cut(s) 490
BstSFI CTRYAG 1 cut(s) 145
BstSLI GKGCMC 3 cut(s) 164, 492, 643
BstV1I GCAGC 5 cut(s) 159, 491, 908, 989, 1021
BstV2I GAAGAC 1 cut(s) 397
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
BsuRI GGCC 2 cut(s) 599, 713
BtsCI GGATG 3 cut(s) 175, 269, 652
BtsIMutI CAGTG 4 cut(s) 162, 164, 270, 559
BveI ACCTGC 1 cut(s) 19
Cac8I GCNNGC 3 cut(s) 484, 488, 1167
CciI TCATGA 1 cut(s) 288
Cfr13I GGNCC 3 cut(s) 275, 711, 995
CseI GACGC 2 cut(s) 657, 781
CviAII CATG 7 cut(s) 46, 289, 316, 351, 609, 688, 829
DdeI CTNAG 2 cut(s) 165, 570
DpnI GATC 5 cut(s) 63, 86, 381, 566, 749
DpnII GATC 5 cut(s) 61, 84, 379, 564, 747
DraIII CACNNNGTG 1 cut(s) 538
DriI GACNNNNNGTC 1 cut(s) 421
Eam1104I CTCTTC 3 cut(s) 170, 230, 449
Eam1105I GACNNNNNGTC 1 cut(s) 421
EarI CTCTTC 3 cut(s) 170, 230, 449
Eco147I AGGCCT 1 cut(s) 599
Eco47I GGWCC 2 cut(s) 275, 995
Eco57I CTGAAG 1 cut(s) 750
Eco88I CYCGRG 1 cut(s) 518
EcoRI GAATTC 1 cut(s) 759
FaeI CATG 7 cut(s) 49, 292, 319, 354, 612, 691, 832
FaqI GGGAC 2 cut(s) 655, 784
FatI CATG 7 cut(s) 45, 288, 315, 350, 608, 687, 828
FbaI TGATCA 1 cut(s) 379
FblI GTMKAC 2 cut(s) 965, 1116
Fnu4HI GCNGC 5 cut(s) 148, 480, 897, 978, 1035
FokI GGATG 3 cut(s) 182, 276, 659
Fsp4HI GCNGC 5 cut(s) 148, 480, 897, 978, 1035
FspBI CTAG 4 cut(s) 308, 708, 822, 905
GluI GCNGC 5 cut(s) 148, 480, 897, 978, 1035
GsaI CCCAGC 1 cut(s) 977
HaeIII GGCC 2 cut(s) 599, 713
HapII CCGG 3 cut(s) 492, 578, 644
HgaI GACGC 2 cut(s) 657, 781
Hin1I GRCGYC 1 cut(s) 773
Hin1II CATG 7 cut(s) 49, 292, 319, 354, 612, 691, 832
HincII GTYRAC 2 cut(s) 966, 1003
HindII GTYRAC 2 cut(s) 966, 1003
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 6 cut(s) 49, 422, 521, 625, 872, 962
HpaII CCGG 3 cut(s) 492, 578, 644
HphI GGTGA 4 cut(s) 439, 497, 620, 1030
Hpy166II GTNNAC 5 cut(s) 162, 641, 966, 1003, 1117
Hpy188I TCNGA 4 cut(s) 376, 606, 784, 987
Hpy188III TCNNGA 6 cut(s) 65, 289, 299, 518, 905, 1140
Hpy8I GTNNAC 5 cut(s) 162, 641, 966, 1003, 1117
HpyAV CCTTC 2 cut(s) 725, 886
HpyCH4III ACNGT 4 cut(s) 110, 128, 159, 509
HpyCH4IV ACGT 1 cut(s) 23
HpyF3I CTNAG 2 cut(s) 165, 570
HpySE526I ACGT 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 773
Hsp92II CATG 7 cut(s) 49, 292, 319, 354, 612, 691, 832
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 5 cut(s) 61, 84, 379, 564, 747
Lsp1109I GCAGC 5 cut(s) 159, 491, 908, 989, 1021
LweI GCATC 1 cut(s) 1133
MaeI CTAG 4 cut(s) 308, 708, 822, 905
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 2 cut(s) 128, 1018
MalI GATC 5 cut(s) 63, 86, 381, 566, 749
MboI GATC 5 cut(s) 61, 84, 379, 564, 747
MboII GAAGA 7 cut(s) 187, 217, 397, 436, 869, 1094, 1107
MfeI CAATTG 1 cut(s) 808
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 3 cut(s) 164, 492, 643
MlyI GAGTC 3 cut(s) 431, 530, 971
MmeI TCCRAC 1 cut(s) 629
MroXI GAANNNNTTC 1 cut(s) 431
MseI TTAA 2 cut(s) 411, 945
MslI CAYNNNNRTG 4 cut(s) 287, 375, 636, 833
MspA1I CMGCKG 2 cut(s) 38, 482
MspI CCGG 3 cut(s) 492, 578, 644
MspR9I CCNGG 1 cut(s) 492
MunI CAATTG 1 cut(s) 808
Mva1269I GAATGC 1 cut(s) 709
NciI CCSGG 1 cut(s) 492
NdeII GATC 5 cut(s) 61, 84, 379, 564, 747
NlaIII CATG 7 cut(s) 49, 292, 319, 354, 612, 691, 832
NmuCI GTSAC 1 cut(s) 1018
OliI CACNNNNGTG 1 cut(s) 636
PagI TCATGA 1 cut(s) 288
PceI AGGCCT 1 cut(s) 599
PctI GAATGC 1 cut(s) 709
PdmI GAANNNNTTC 1 cut(s) 431
PfeI GAWTC 3 cut(s) 49, 625, 872
PflMI CCANNNNNTGG 1 cut(s) 679
PkrI GCNGC 5 cut(s) 149, 481, 898, 979, 1036
PleI GAGTC 3 cut(s) 430, 529, 970
PpsI GAGTC 3 cut(s) 430, 529, 970
Psp1406I AACGTT 1 cut(s) 23
PspFI CCCAGC 1 cut(s) 973
PspPI GGNCC 3 cut(s) 275, 711, 995
PstI CTGCAG 1 cut(s) 149
PsuI RGATCY 1 cut(s) 564
PvuII CAGCTG 2 cut(s) 38, 482
RseI CAYNNNNRTG 4 cut(s) 287, 375, 636, 833
SalI GTCGAC 1 cut(s) 964
SaqAI TTAA 2 cut(s) 411, 945
SatI GCNGC 5 cut(s) 148, 480, 897, 978, 1035
Sau3AI GATC 5 cut(s) 61, 84, 379, 564, 747
Sau96I GGNCC 3 cut(s) 275, 711, 995
SchI GAGTC 3 cut(s) 431, 530, 971
ScrFI CCNGG 1 cut(s) 492
SduI GDGCHC 3 cut(s) 164, 492, 643
SfaNI GCATC 1 cut(s) 1133
SfcI CTRYAG 1 cut(s) 145
SinI GGWCC 2 cut(s) 275, 995
SmiMI CAYNNNNRTG 4 cut(s) 287, 375, 636, 833
SmlI CTYRAG 1 cut(s) 1175
SmoI CTYRAG 1 cut(s) 1175
SseBI AGGCCT 1 cut(s) 599
SsiI CCGC 1 cut(s) 254
SspMI CTAG 4 cut(s) 308, 708, 822, 905
StuI AGGCCT 1 cut(s) 599
StyD4I CCNGG 1 cut(s) 490
TaaI ACNGT 4 cut(s) 110, 128, 159, 509
TaiI ACGT 1 cut(s) 26
TaqI TCGA 2 cut(s) 425, 965
TaqII GACCGA 1 cut(s) 1118
TfiI GAWTC 3 cut(s) 49, 625, 872
Tru1I TTAA 2 cut(s) 411, 945
Tru9I TTAA 2 cut(s) 411, 945
TscAI CASTG 4 cut(s) 164, 169, 277, 566
TseFI GTSAC 1 cut(s) 1018
TseI GCWGC 5 cut(s) 147, 479, 896, 977, 1034
Tsp45I GTSAC 1 cut(s) 1018
TspDTI ATGAA 6 cut(s) 62, 119, 175, 230, 277, 663
TspGWI ACGGA 1 cut(s) 72
TspRI CASTG 4 cut(s) 164, 169, 277, 566
Van91I CCANNNNNTGG 1 cut(s) 679
VneI GTGCAC 2 cut(s) 160, 639
VpaK11BI GGWCC 2 cut(s) 275, 995
XapI RAATTY 5 cut(s) 182, 395, 694, 759, 786
XbaI TCTAGA 1 cut(s) 904
XmiI GTMKAC 2 cut(s) 965, 1116
XmnI GAANNNNTTC 1 cut(s) 431
XspI CTAG 4 cut(s) 308, 708, 822, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.