MD16G1017000.v1.1

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008919
Physical Location & Seq
Forward (+)
1267715 .. 1268362
648 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1017000.v1.1.491

Sequence Viewer

Length: 321 bp
ATGGCAGAAGGGGGCCAAGGAACGTCGCAGGTTTCAGCTGTTTCCGTGAATCTCAACGGAGATCGTGAAAATAGTGCAAAACCGGTCACGGTTTCGCTGCCTATGAACGGTCGAAATGGAATTTACAGCTACTCCAGGAACTCCTACCACCAGAAACTATCCGCTGGACTTGAGAAGGGAAAAATCATCGAGGAAATTGAGCAGAAGCTTGATATGAAAAAGCTCTCCTCCTCTTCAAACACCATTCATGTTGCGGACTTGGGATGTTCCACCGGACCAAACACTTTCATGATAATTCATGATATACTAGAAGCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

107

Amino Acids

11.4

Weight (kDa)

6.26

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 73 - 106 2.8e-09 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 19
AciI CCGC 2 cut(s) 162, 254
AcsI RAATTY 1 cut(s) 120
AfiI CCNNNNNNNGG 1 cut(s) 107
AgeI ACCGGT 1 cut(s) 82
AgsI TTSAA 1 cut(s) 237
AjnI CCWGG 1 cut(s) 134
AluBI AGCT 4 cut(s) 38, 129, 208, 223
AluI AGCT 4 cut(s) 38, 129, 208, 223
AoxI GGCC 1 cut(s) 13
ApeKI GCWGC 1 cut(s) 97
ApoI RAATTY 1 cut(s) 120
AsiGI ACCGGT 1 cut(s) 82
AspS9I GGNCC 2 cut(s) 13, 275
AvaII GGWCC 1 cut(s) 275
BbvI GCAGC 1 cut(s) 84
BciT130I CCWGG 1 cut(s) 136
BfaI CTAG 1 cut(s) 308
BfuAI ACCTGC 1 cut(s) 19
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
Bme1390I CCNGG 1 cut(s) 136
Bme18I GGWCC 1 cut(s) 275
BmgT120I GGNCC 2 cut(s) 13, 275
BmiI GGNNCC 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 136
BpmI CTGGAG 1 cut(s) 118
BpuEI CTTGAG 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 16
BsaWI WCCGGW 2 cut(s) 82, 272
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 107
Bse118I RCCGGY 1 cut(s) 82
BseBI CCWGG 1 cut(s) 136
BseDI CCNNGG 1 cut(s) 16
BseGI GGATG 1 cut(s) 269
BseLI CCNNNNNNNGG 1 cut(s) 107
BseRI GAGGAG 2 cut(s) 217, 220
BseXI GCAGC 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 1 cut(s) 15
BshTI ACCGGT 1 cut(s) 82
BsiEI CGRYCG 1 cut(s) 112
BsiSI CCGG 2 cut(s) 83, 273
BslI CCNNNNNNNGG 1 cut(s) 107
BsnI GGCC 1 cut(s) 15
Bsp143I GATC 1 cut(s) 61
BspACI CCGC 2 cut(s) 162, 254
BspANI GGCC 1 cut(s) 15
BspHI TCATGA 2 cut(s) 288, 298
BspLI GGNNCC 1 cut(s) 14
BspMI ACCTGC 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 1 cut(s) 16
BssMI GATC 1 cut(s) 61
BssT1I CCWWGG 1 cut(s) 16
Bst2UI CCWGG 1 cut(s) 136
Bst4CI ACNGT 2 cut(s) 91, 110
Bst6I CTCTTC 1 cut(s) 238
BstF5I GGATG 1 cut(s) 269
BstKTI GATC 1 cut(s) 64
BstMBI GATC 1 cut(s) 61
BstMCI CGRYCG 1 cut(s) 112
BstNI CCWGG 1 cut(s) 136
BstSCI CCNGG 1 cut(s) 134
BstV1I GCAGC 1 cut(s) 84
BsuRI GGCC 1 cut(s) 15
BtsCI GGATG 1 cut(s) 269
BveI ACCTGC 1 cut(s) 19
CciI TCATGA 2 cut(s) 288, 298
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 2 cut(s) 13, 275
CspAI ACCGGT 1 cut(s) 82
CviAII CATG 4 cut(s) 248, 289, 299, 316
CviJI RGCY 6 cut(s) 15, 38, 129, 208, 223, 314
CviKI_1 RGCY 6 cut(s) 15, 38, 129, 208, 223, 314
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 238
EarI CTCTTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 134
EcoT14I CCWWGG 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 16
FaeI CATG 4 cut(s) 251, 292, 302, 319
FaiI YATR 7 cut(s) 104, 215, 249, 290, 300, 305, 317
FatI CATG 4 cut(s) 247, 288, 298, 315
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 1 cut(s) 276
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 1 cut(s) 308
GluI GCNGC 1 cut(s) 98
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 1 cut(s) 15
HapII CCGG 2 cut(s) 83, 273
Hin1II CATG 4 cut(s) 251, 292, 302, 319
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 1 cut(s) 49
HpaII CCGG 2 cut(s) 83, 273
Hpy188III TCNNGA 3 cut(s) 65, 289, 299
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 1 cut(s) 169
HpyCH4III ACNGT 2 cut(s) 91, 110
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 1 cut(s) 77
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 4 cut(s) 251, 292, 302, 319
Kzo9I GATC 1 cut(s) 61
LpnPI CCDG 7 cut(s) 14, 96, 121, 148, 150, 164, 286
Lsp1109I GCAGC 1 cut(s) 84
MaeI CTAG 1 cut(s) 308
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 1 cut(s) 85
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MboII GAAGA 1 cut(s) 225
MluCI AATT 3 cut(s) 120, 195, 294
MnlI CCTC 3 cut(s) 184, 238, 241
MslI CAYNNNNRTG 1 cut(s) 287
MspA1I CMGCKG 2 cut(s) 38, 164
MspI CCGG 2 cut(s) 83, 273
MspR9I CCNGG 1 cut(s) 136
MvaI CCWGG 1 cut(s) 136
NdeII GATC 1 cut(s) 61
NlaIII CATG 4 cut(s) 251, 292, 302, 319
NlaIV GGNNCC 1 cut(s) 14
NmuCI GTSAC 1 cut(s) 85
PagI TCATGA 2 cut(s) 288, 298
PfeI GAWTC 1 cut(s) 49
PfoI TCCNGGA 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 82
PkrI GCNGC 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 134
PspGI CCWGG 1 cut(s) 134
PspN4I GGNNCC 1 cut(s) 14
PspPI GGNCC 2 cut(s) 13, 275
PvuII CAGCTG 1 cut(s) 38
RseI CAYNNNNRTG 1 cut(s) 287
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 1 cut(s) 61
Sau96I GGNCC 2 cut(s) 13, 275
ScrFI CCNGG 1 cut(s) 136
SetI ASST 6 cut(s) 26, 33, 40, 131, 210, 225
SinI GGWCC 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 3 cut(s) 120, 195, 294
SsiI CCGC 2 cut(s) 162, 254
SspMI CTAG 1 cut(s) 308
StyD4I CCNGG 1 cut(s) 134
StyI CCWWGG 1 cut(s) 16
TaaI ACNGT 2 cut(s) 91, 110
TaiI ACGT 1 cut(s) 26
TaqI TCGA 2 cut(s) 112, 189
TasI AATT 3 cut(s) 120, 195, 294
TfiI GAWTC 1 cut(s) 49
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 1 cut(s) 97
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 5 cut(s) 119, 230, 236, 277, 287
TspGWI ACGGA 2 cut(s) 34, 72
VpaK11BI GGWCC 1 cut(s) 275
XapI RAATTY 1 cut(s) 120
XspI CTAG 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.