MD13G1019200.v1.1

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008919
Physical Location & Seq
Forward (+)
1210837 .. 1213581
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1019200.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGGCAGAAGGGGATCGAACGATGTTGTCGGGTTCAGCTGATCCCATGAATCTCAACGGAGATCATGAAAATAGAGCGAAACCGGTCACAGGTCCGCTGCCTATGAACGGTGGAAATGGCACTTACAGCTACTCCAAGAACTCCTCCTACCAGAAAGAATCTGCTGATCTTGAGAAGGGAAAAATCATTGAGGAAATTGAGGAGAAGCTTGATATGAAAAAACTCTCATCTTTTTCAAACACCATTTGTCTAGCAGACTTGGGATGTTCCACTGGACCAAACACTTTCATGATAATTCAAGAAATACTAGAAGCTATGCAACGCAAACACCGATCCCAATTATCCCAATCATGTCCCAAAGCTGATCAACAGTACTCTGATGATCATCAAATGCCTGAATTTCAAGTCTTCTTTAACGACCTAGAGGCGAATGACTTCAATACCCTCTTCACCTCTCTCCCACAAGACAGGAAATACTTTGCAGCCGGCGTGCCCGGTTCTTTCCATCGCCGTTTGTTTCCCGAGTCGTCTATCCACTTTGTACATACCTCATCTAGTCTTCATTGGATCTCCAAGTCGCCTGAAGTATTGCAAAACAAGGACTCTCCGGCGTGGAACAAGGGCAGGATTCATTACACAAGTGCCCCGGATGAAGTGGTTGATGCTTACGCGGCGCAGTTTGCCGAGGACATGAAGAACTTTTTGAATGTTAGGGCCACAGAGCTTGTGCCTGGAGGAATGATGGTAATGATCTTGTCTGGAATCCCCAAAGGGATGCCTTATTCTGAAATCCCAGCGGGTATGTTGTACAATTGTATATCGTCTAGCCTCATGGATATGGCAAAAGAGGGAATAATTGAAGAAAGTGAAGTGGATTCCTTCAACTTGCCATATTATGCAGCCTCGTTAGAGGAGATGGAGGAGATAGTAGAGAAAAACGGGTGTTTTAAGATAGAGAGAATGGAGTCGTCAAACCCAGCAGCATGGCTGCAAAATCGTCCAGTTGACATACCAACTTTGGTGAAACATGTAAGGGCTGCAGTGGAGGGGATGTTTGCCAGACACTTTGGAAGTGAGGTCATGGATGAAATGTTCGGAAGAGTGACCGATAAACTTCTAGACATTTCCGACCTCGTAAACTCACGGCGCGACGAGAAAAGTCAGTTGCTTGCTGTTCTCAAACGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

397

Amino Acids

44.43

Weight (kDa)

5.15

Isoelectric Point (pI)

50.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 73 - 395 4.1e-108 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 671, 1149
AciI CCGC 3 cut(s) 95, 671, 797
AclWI GGATC 4 cut(s) 21, 35, 327, 575
AcsI RAATTY 1 cut(s) 398
AcuI CTGAAG 1 cut(s) 603
AfaI GTAC 3 cut(s) 374, 543, 809
AfiI CCNNNNNNNGG 2 cut(s) 89, 107
AflIII ACRYGT 1 cut(s) 1027
AgeI ACCGGT 1 cut(s) 82
AgsI TTSAA 7 cut(s) 237, 299, 404, 439, 706, 860, 883
AjnI CCWGG 1 cut(s) 730
AloI GAACNNNNNNTCC 2 cut(s) 1076, 1108
AluBI AGCT 6 cut(s) 38, 129, 208, 314, 362, 724
AluI AGCT 6 cut(s) 38, 129, 208, 314, 362, 724
AlwI GGATC 4 cut(s) 21, 35, 327, 575
Ama87I CYCGRG 1 cut(s) 521
AoxI GGCC 1 cut(s) 714
ApeKI GCWGC 6 cut(s) 97, 482, 899, 980, 988, 1037
ApoI RAATTY 1 cut(s) 398
AsiGI ACCGGT 1 cut(s) 82
Asp700I GAANNNNTTC 1 cut(s) 434
AspLEI GCGC 2 cut(s) 676, 1149
AspS9I GGNCC 3 cut(s) 92, 275, 714
AsuC2I CCSGG 2 cut(s) 495, 647
AsuHPI GGTGA 2 cut(s) 442, 1033
AvaI CYCGRG 1 cut(s) 521
AvaII GGWCC 2 cut(s) 92, 275
BaeGI GKGCMC 2 cut(s) 495, 646
BbsI GAAGAC 2 cut(s) 400, 551
BbvI GCAGC 6 cut(s) 84, 494, 911, 975, 992, 1024
BccI CCATC 3 cut(s) 513, 736, 910
BceAI ACGGC 2 cut(s) 495, 1160
BciT130I CCWGG 1 cut(s) 732
BclI TGATCA 2 cut(s) 364, 382
BcnI CCSGG 2 cut(s) 495, 647
BfaI CTAG 6 cut(s) 251, 308, 422, 555, 825, 1118
BfmI CTRYAG 1 cut(s) 1038
BisI GCNGC 7 cut(s) 98, 483, 672, 900, 981, 989, 1038
BlsI GCNGC 7 cut(s) 99, 484, 673, 901, 982, 990, 1039
BmcAI AGTACT 1 cut(s) 374
Bme1390I CCNGG 3 cut(s) 495, 647, 732
Bme18I GGWCC 2 cut(s) 92, 275
BmeT110I CYCGRG 1 cut(s) 521
BmgT120I GGNCC 3 cut(s) 92, 275, 714
BmrFI CCNGG 3 cut(s) 495, 647, 732
BmsI GCATC 2 cut(s) 652, 765
BpiI GAAGAC 2 cut(s) 400, 551
BpmI CTGGAG 1 cut(s) 753
BpuEI CTTGAG 1 cut(s) 191
BpuMI CCSGG 2 cut(s) 495, 647
BsaBI GATNNNNATC 1 cut(s) 384
BsaJI CCNNGG 2 cut(s) 645, 684
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 4 cut(s) 116, 146, 1037, 1067
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 107
Bse118I RCCGGY 2 cut(s) 82, 485
Bse1I ACTGG 2 cut(s) 277, 1001
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 1 cut(s) 732
BseDI CCNNGG 2 cut(s) 645, 684
BseGI GGATG 5 cut(s) 269, 655, 780, 1056, 1090
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 2 cut(s) 89, 107
BseNI ACTGG 2 cut(s) 277, 1001
BseRI GAGGAG 4 cut(s) 133, 215, 926, 935
BseSI GKGCMC 2 cut(s) 495, 646
BseXI GCAGC 6 cut(s) 84, 494, 911, 975, 992, 1024
BseYI CCCAGC 2 cut(s) 793, 976
Bsh1236I CGCG 2 cut(s) 671, 1149
BshFI GGCC 1 cut(s) 716
BshTI ACCGGT 1 cut(s) 82
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 5 cut(s) 83, 486, 495, 608, 647
BslFI GGGAC 1 cut(s) 339
BslI CCNNNNNNNGG 2 cut(s) 89, 107
BsmFI GGGAC 1 cut(s) 339
BsnI GGCC 1 cut(s) 716
BsoBI CYCGRG 1 cut(s) 521
Bsp1286I GDGCHC 2 cut(s) 495, 646
Bsp1407I TGTACA 2 cut(s) 541, 807
Bsp143I GATC 9 cut(s) 13, 40, 61, 166, 332, 364, 382, 567, 750
BspACI CCGC 3 cut(s) 95, 671, 797
BspANI GGCC 1 cut(s) 716
BspFNI CGCG 2 cut(s) 671, 1149
BspHI TCATGA 2 cut(s) 64, 288
BspMAI CTGCAG 1 cut(s) 1042
BspPI GGATC 4 cut(s) 21, 35, 327, 575
BsrFI RCCGGY 2 cut(s) 82, 485
BsrGI TGTACA 2 cut(s) 541, 807
BsrI ACTGG 2 cut(s) 277, 1001
BssAI RCCGGY 2 cut(s) 82, 485
BssECI CCNNGG 2 cut(s) 645, 684
BssMI GATC 9 cut(s) 13, 40, 61, 166, 332, 364, 382, 567, 750
Bst2UI CCWGG 1 cut(s) 732
Bst4CI ACNGT 2 cut(s) 110, 372
Bst6I CTCTTC 2 cut(s) 452, 1093
BstAUI TGTACA 2 cut(s) 541, 807
BstC8I GCNNGC 3 cut(s) 487, 491, 1170
BstF5I GGATG 5 cut(s) 269, 655, 780, 1056, 1090
BstFNI CGCG 2 cut(s) 671, 1149
BstHHI GCGC 2 cut(s) 676, 1149
BstKTI GATC 9 cut(s) 16, 43, 64, 169, 335, 367, 385, 570, 753
BstMBI GATC 9 cut(s) 13, 40, 61, 166, 332, 364, 382, 567, 750
BstMWI GCNNNNNNNGC 3 cut(s) 126, 671, 680
BstNI CCWGG 1 cut(s) 732
BstNSI RCATGY 1 cut(s) 1031
BstSCI CCNGG 3 cut(s) 493, 645, 730
BstSFI CTRYAG 1 cut(s) 1038
BstSLI GKGCMC 2 cut(s) 495, 646
BstUI CGCG 2 cut(s) 671, 1149
BstV1I GCAGC 6 cut(s) 84, 494, 911, 975, 992, 1024
BstV2I GAAGAC 2 cut(s) 400, 551
BstX2I RGATCY 1 cut(s) 567
BstXI CCANNNNNNTGG 1 cut(s) 984
BstYI RGATCY 1 cut(s) 567
BsuRI GGCC 1 cut(s) 716
BtgZI GCGATG 1 cut(s) 491
BtsCI GGATG 5 cut(s) 269, 655, 780, 1056, 1090
BtsI GCAGTG 1 cut(s) 1047
BtsIMutI CAGTG 2 cut(s) 270, 1047
Cac8I GCNNGC 3 cut(s) 487, 491, 1170
CciI TCATGA 2 cut(s) 64, 288
CfoI GCGC 2 cut(s) 676, 1149
Cfr10I RCCGGY 2 cut(s) 82, 485
Cfr13I GGNCC 3 cut(s) 92, 275, 714
Csp6I GTAC 3 cut(s) 373, 542, 808
CspAI ACCGGT 1 cut(s) 82
CspCI CAANNNNNGTGG 2 cut(s) 706, 741
CviAII CATG 9 cut(s) 46, 65, 289, 351, 691, 832, 984, 1028, 1081
CviQI GTAC 3 cut(s) 373, 542, 808
DpnI GATC 9 cut(s) 15, 42, 63, 168, 334, 366, 384, 569, 752
DpnII GATC 9 cut(s) 13, 40, 61, 166, 332, 364, 382, 567, 750
Eam1104I CTCTTC 2 cut(s) 452, 1093
EarI CTCTTC 2 cut(s) 452, 1093
Eco47I GGWCC 2 cut(s) 92, 275
Eco57I CTGAAG 1 cut(s) 603
Eco88I CYCGRG 1 cut(s) 521
EcoRII CCWGG 1 cut(s) 730
FaeI CATG 9 cut(s) 49, 68, 292, 354, 694, 835, 987, 1031, 1084
FaqI GGGAC 1 cut(s) 339
FatI CATG 9 cut(s) 45, 64, 288, 350, 690, 831, 983, 1027, 1080
FauI CCCGC 1 cut(s) 790
FbaI TGATCA 2 cut(s) 364, 382
Fnu4HI GCNGC 7 cut(s) 98, 483, 672, 900, 981, 989, 1038
FokI GGATG 5 cut(s) 276, 662, 787, 1063, 1097
Fsp4HI GCNGC 7 cut(s) 98, 483, 672, 900, 981, 989, 1038
FspBI CTAG 6 cut(s) 251, 308, 422, 555, 825, 1118
GlaI GCGC 2 cut(s) 675, 1148
GluI GCNGC 7 cut(s) 98, 483, 672, 900, 981, 989, 1038
GsaI CCCAGC 2 cut(s) 797, 980
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 1 cut(s) 716
HapII CCGG 5 cut(s) 83, 486, 495, 608, 647
HhaI GCGC 2 cut(s) 676, 1149
Hin1II CATG 9 cut(s) 49, 68, 292, 354, 694, 835, 987, 1031, 1084
Hin6I GCGC 2 cut(s) 674, 1147
HinP1I GCGC 2 cut(s) 674, 1147
HincII GTYRAC 1 cut(s) 1006
HindII GTYRAC 1 cut(s) 1006
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 8 cut(s) 49, 158, 524, 602, 628, 762, 875, 965
HpaII CCGG 5 cut(s) 83, 486, 495, 608, 647
HphI GGTGA 2 cut(s) 442, 1033
Hpy166II GTNNAC 2 cut(s) 1006, 1138
Hpy188I TCNGA 4 cut(s) 379, 787, 1097, 1129
Hpy188III TCNNGA 7 cut(s) 65, 170, 289, 299, 521, 759, 1118
Hpy8I GTNNAC 2 cut(s) 1006, 1138
Hpy99I CGWCG 1 cut(s) 1154
HpyAV CCTTC 2 cut(s) 169, 889
HpyCH4III ACNGT 2 cut(s) 110, 372
HpyCH4V TGCA 6 cut(s) 319, 482, 592, 899, 991, 1040
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 671, 680
Hsp92II CATG 9 cut(s) 49, 68, 292, 354, 694, 835, 987, 1031, 1084
HspAI GCGC 2 cut(s) 674, 1147
KroI GCCGGC 1 cut(s) 485
KroNI GCCGGC 1 cut(s) 487
Ksp22I TGATCA 2 cut(s) 364, 382
Kzo9I GATC 9 cut(s) 13, 40, 61, 166, 332, 364, 382, 567, 750
Lsp1109I GCAGC 6 cut(s) 84, 494, 911, 975, 992, 1024
LweI GCATC 2 cut(s) 652, 765
MaeI CTAG 6 cut(s) 251, 308, 422, 555, 825, 1118
MaeIII GTNAC 2 cut(s) 85, 1102
MalI GATC 9 cut(s) 15, 42, 63, 168, 334, 366, 384, 569, 752
MboI GATC 9 cut(s) 13, 40, 61, 166, 332, 364, 382, 567, 750
MboII GAAGA 6 cut(s) 400, 439, 551, 706, 872, 1110
MfeI CAATTG 1 cut(s) 811
MflI RGATCY 1 cut(s) 567
MhlI GDGCHC 2 cut(s) 495, 646
MluCI AATT 6 cut(s) 195, 294, 338, 398, 811, 855
MlyI GAGTC 3 cut(s) 533, 596, 974
MmeI TCCRAC 1 cut(s) 1152
MroNI GCCGGC 1 cut(s) 485
MroXI GAANNNNTTC 1 cut(s) 434
MseI TTAA 2 cut(s) 414, 948
MslI CAYNNNNRTG 2 cut(s) 287, 836
MspA1I CMGCKG 3 cut(s) 38, 97, 797
MspI CCGG 5 cut(s) 83, 486, 495, 608, 647
MspR9I CCNGG 3 cut(s) 495, 647, 732
MunI CAATTG 1 cut(s) 811
MvaI CCWGG 1 cut(s) 732
MvnI CGCG 2 cut(s) 671, 1149
MwoI GCNNNNNNNGC 3 cut(s) 126, 671, 680
NaeI GCCGGC 1 cut(s) 487
NciI CCSGG 2 cut(s) 495, 647
NdeII GATC 9 cut(s) 13, 40, 61, 166, 332, 364, 382, 567, 750
NgoMIV GCCGGC 1 cut(s) 485
NlaIII CATG 9 cut(s) 49, 68, 292, 354, 694, 835, 987, 1031, 1084
NmeAIII GCCGAG 1 cut(s) 709
NmuCI GTSAC 2 cut(s) 85, 1102
NspI RCATGY 1 cut(s) 1031
PagI TCATGA 2 cut(s) 64, 288
PciI ACATGT 1 cut(s) 1027
PcsI WCGNNNNNNNCGW 1 cut(s) 328
PdiI GCCGGC 1 cut(s) 487
PdmI GAANNNNTTC 1 cut(s) 434
PfeI GAWTC 5 cut(s) 49, 158, 628, 762, 875
PinAI ACCGGT 1 cut(s) 82
PkrI GCNGC 7 cut(s) 99, 484, 673, 901, 982, 990, 1039
PleI GAGTC 3 cut(s) 532, 596, 973
PpsI GAGTC 3 cut(s) 532, 596, 973
PscI ACATGT 1 cut(s) 1027
Psp6I CCWGG 1 cut(s) 730
PspFI CCCAGC 2 cut(s) 793, 976
PspGI CCWGG 1 cut(s) 730
PspPI GGNCC 3 cut(s) 92, 275, 714
PsrI GAACNNNNNNTAC 2 cut(s) 131, 163
PstI CTGCAG 1 cut(s) 1042
PsuI RGATCY 1 cut(s) 567
PvuII CAGCTG 1 cut(s) 38
RsaI GTAC 3 cut(s) 374, 543, 809
RsaNI GTAC 3 cut(s) 373, 542, 808
RseI CAYNNNNRTG 2 cut(s) 287, 836
SaqAI TTAA 2 cut(s) 414, 948
SatI GCNGC 7 cut(s) 98, 483, 672, 900, 981, 989, 1038
Sau3AI GATC 9 cut(s) 13, 40, 61, 166, 332, 364, 382, 567, 750
Sau96I GGNCC 3 cut(s) 92, 275, 714
ScaI AGTACT 1 cut(s) 374
SchI GAGTC 3 cut(s) 533, 596, 974
ScrFI CCNGG 3 cut(s) 495, 647, 732
SduI GDGCHC 2 cut(s) 495, 646
SfaNI GCATC 2 cut(s) 652, 765
SfcI CTRYAG 1 cut(s) 1038
SinI GGWCC 2 cut(s) 92, 275
SmiMI CAYNNNNRTG 2 cut(s) 287, 836
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 6 cut(s) 195, 294, 338, 398, 811, 855
SsiI CCGC 3 cut(s) 95, 671, 797
SspMI CTAG 6 cut(s) 251, 308, 422, 555, 825, 1118
StyD4I CCNGG 3 cut(s) 493, 645, 730
TaaI ACNGT 2 cut(s) 110, 372
TaqI TCGA 1 cut(s) 16
TaqII GACCGA 1 cut(s) 1121
TasI AATT 6 cut(s) 195, 294, 338, 398, 811, 855
TatI WGTACW 3 cut(s) 372, 541, 807
TauI GCSGC 1 cut(s) 674
TfiI GAWTC 5 cut(s) 49, 158, 628, 762, 875
Tru1I TTAA 2 cut(s) 414, 948
Tru9I TTAA 2 cut(s) 414, 948
TscAI CASTG 2 cut(s) 277, 1047
TseFI GTSAC 2 cut(s) 85, 1102
TseI GCWGC 6 cut(s) 97, 482, 899, 980, 988, 1037
Tsp45I GTSAC 2 cut(s) 85, 1102
TspGWI ACGGA 1 cut(s) 72
TspRI CASTG 2 cut(s) 277, 1047
VpaK11BI GGWCC 2 cut(s) 92, 275
XapI RAATTY 1 cut(s) 398
XbaI TCTAGA 1 cut(s) 1117
XceI RCATGY 1 cut(s) 1031
XmnI GAANNNNTTC 1 cut(s) 434
XspI CTAG 6 cut(s) 251, 308, 422, 555, 825, 1118
ZrmI AGTACT 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.