Rw4G034100

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008919
Physical Location & Seq
Forward (+)
61207097 .. 61209533
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034100.1

Sequence Viewer

Length: 1107 bp
ATGAATGGAAATAATTGCACGAAATCGATAACAGGTTCAATGCCAATGAACGGTGGAAACGGCACTCACAGCTACTCCAAGAACTCCTACTACCAGAAAAAAGCTGCAGAGATTGAAAAGGTAAAGATCATGGAGGTGATTGCTGAGAAGCTTGATATGAAAAACCTCTCCTCAATGTCAAGCACTATTCGTATTGCAGACTTGGGATGTTCAACTGGGCCAAACACTTTCGTGACAATGCAAAACATACTAGAAGCCATGCAACAAAAGTACCGATCCCAATCTGCTCCTGATGATCATCAAACAATGCCTGAATTTCAAGTGTTCTTCAACGACCTAGTGTTGAATGACTTCAATACCCTGTTTACCTCTCTTCCCAAAGGCAAGCAATACTTTGCAGCAGGGGTACCGGGGTCTTTTCACGACCGTCTGTTTCCGGAGTCCTCTATCCACCTTGTGCACGCCTCACTTTCCCTCCACTGGCTCTCTAAGTTGCCAGAAGAGTTGCAAGACAAGGCCTCTCCAGCATGGAACAAAGGGAGGATTCACTACACAAGTGCCCCTAATGAAGTGGTTGATGCTTACATGTCCCAGTTTGCCAAGGAAATGGAGAACTTTCTGGATGCGAGGGCTAAAGAGCTTGTGCCTGGAGGAATGATGGTGATGGTCTTGTCTGGAAATCATAAAGGCATGCCTTATTCTCAAACCGCATCCGGCATGATGTACGATGCGATATCATCGAGCCTCATGGATATGGAAAAAGAGGGATTGATTGATGAAGGTCAAGTGGATGCTTTCAATTTGCCTTTCTACGCTGCCTCACTGGAGGAGTTGGAGGGGCTTGTGGTTAAAAATGGGTGCTTTAGCATTGAGAGGATGGAGTCTTCAAACCCAGCAGCATGGATGAAAGGTCCACTTGACATAACACAATGGGTGATGCATATACGGGCTGCAATGGAAGGAATGTTCGCCGGACATTTTGGAAGTCATGAGGTTATGGATCAAATGTTCCAACGTTCGGCTCAAAAGCTTTCAGACAAGTCTGACCTTATAAACTCAAGGTGCCACGAGCAAAGTCAGCTGCTTGCTGTTCTCAAGCGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

368

Amino Acids

41.08

Weight (kDa)

5.85

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 55 - 367 2e-107 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1052
Acc65I GGTACC 1 cut(s) 406
AccB1I GGYRCC 2 cut(s) 406, 1062
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 1 cut(s) 708
AclI AACGTT 1 cut(s) 1015
AclWI GGATC 2 cut(s) 270, 1008
AcsI RAATTY 1 cut(s) 314
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 3 cut(s) 272, 408, 725
AfiI CCNNNNNNNGG 3 cut(s) 50, 480, 1018
AflIII ACRYGT 1 cut(s) 585
AgsI TTSAA 9 cut(s) 39, 116, 213, 320, 331, 346, 355, 799, 888
AjnI CCWGG 1 cut(s) 646
AleI CACNNNNGTG 1 cut(s) 230
AloI GAACNNNNNNTCC 4 cut(s) 950, 982, 992, 1024
AluBI AGCT 6 cut(s) 72, 104, 151, 640, 1030, 1081
AluI AGCT 6 cut(s) 72, 104, 151, 640, 1030, 1081
Alw21I GWGCWC 1 cut(s) 462
Alw44I GTGCAC 1 cut(s) 458
AlwI GGATC 2 cut(s) 270, 1008
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 2 cut(s) 218, 516
ApaLI GTGCAC 1 cut(s) 458
ApeKI GCWGC 6 cut(s) 104, 398, 815, 896, 950, 1081
ApoI RAATTY 1 cut(s) 314
Asp700I GAANNNNTTC 1 cut(s) 350
Asp718I GGTACC 1 cut(s) 406
AspS9I GGNCC 2 cut(s) 218, 911
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 3 cut(s) 148, 673, 946
AvaII GGWCC 1 cut(s) 911
BaeGI GKGCMC 2 cut(s) 462, 562
BaeI ACNNNNGTAYC 4 cut(s) 254, 287, 398, 431
BanI GGYRCC 2 cut(s) 406, 1062
BauI CACGAG 1 cut(s) 1067
BbsI GAAGAC 1 cut(s) 876
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 6 cut(s) 91, 410, 802, 908, 937, 1068
BccI CCATC 3 cut(s) 652, 658, 871
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 1 cut(s) 648
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 1 cut(s) 411
BfaI CTAG 2 cut(s) 251, 338
BfmI CTRYAG 1 cut(s) 105
BisI GCNGC 6 cut(s) 105, 399, 816, 897, 951, 1082
BlsI GCNGC 6 cut(s) 106, 400, 817, 898, 952, 1083
Bme1390I CCNGG 2 cut(s) 411, 648
Bme18I GGWCC 1 cut(s) 911
BmgT120I GGNCC 2 cut(s) 218, 911
BmiI GGNNCC 2 cut(s) 408, 1064
BmrFI CCNGG 2 cut(s) 411, 648
BmrI ACTGGG 2 cut(s) 225, 586
BmsI GCATC 6 cut(s) 568, 613, 718, 719, 781, 927
BmuI ACTGGG 2 cut(s) 225, 586
BpiI GAAGAC 1 cut(s) 876
BpmI CTGGAG 3 cut(s) 507, 669, 845
BpuEI CTTGAG 2 cut(s) 1042, 1079
BpuMI CCSGG 1 cut(s) 411
Bsa29I ATCGAT 1 cut(s) 26
BsaBI GATNNNNATC 2 cut(s) 280, 297
BsaJI CCNNGG 2 cut(s) 410, 600
BsaWI WCCGGW 1 cut(s) 436
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 459, 489
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 480, 1018
Bse1I ACTGG 4 cut(s) 220, 485, 592, 828
Bse3DI GCAATG 1 cut(s) 960
Bse8I GATNNNNATC 2 cut(s) 280, 297
BseAI TCCGGA 1 cut(s) 436
BseBI CCWGG 1 cut(s) 648
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 410, 600
BseGI GGATG 6 cut(s) 212, 628, 710, 796, 882, 909
BseJI GATNNNNATC 2 cut(s) 280, 297
BseLI CCNNNNNNNGG 3 cut(s) 50, 480, 1018
BseMI GCAATG 1 cut(s) 960
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 4 cut(s) 220, 485, 592, 828
BseRI GAGGAG 2 cut(s) 160, 842
BseSI GKGCMC 2 cut(s) 462, 562
BseXI GCAGC 6 cut(s) 91, 410, 802, 908, 937, 1068
BseYI CCCAGC 1 cut(s) 892
Bsh1285I CGRYCG 1 cut(s) 427
BshFI GGCC 2 cut(s) 220, 518
BshNI GGYRCC 2 cut(s) 406, 1062
BshVI ATCGAT 1 cut(s) 26
BsiEI CGRYCG 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 462
BsiSI CCGG 4 cut(s) 410, 437, 714, 972
BslFI GGGAC 1 cut(s) 574
BslI CCNNNNNNNGG 3 cut(s) 50, 480, 1018
BsmFI GGGAC 1 cut(s) 574
BsnI GGCC 2 cut(s) 220, 518
Bsp1286I GDGCHC 2 cut(s) 462, 562
Bsp13I TCCGGA 1 cut(s) 436
Bsp143I GATC 4 cut(s) 126, 275, 295, 1000
BspACI CCGC 1 cut(s) 708
BspANI GGCC 2 cut(s) 220, 518
BspCNI CTCAG 1 cut(s) 136
BspDI ATCGAT 1 cut(s) 26
BspEI TCCGGA 1 cut(s) 436
BspHI TCATGA 1 cut(s) 988
BspLI GGNNCC 2 cut(s) 408, 1064
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 2 cut(s) 270, 1008
BspT107I GGYRCC 2 cut(s) 406, 1062
BsrDI GCAATG 1 cut(s) 960
BsrI ACTGG 4 cut(s) 220, 485, 592, 828
BssECI CCNNGG 2 cut(s) 410, 600
BssMI GATC 4 cut(s) 126, 275, 295, 1000
BssSI CACGAG 1 cut(s) 1067
BssT1I CCWWGG 1 cut(s) 600
Bst2BI CACGAG 1 cut(s) 1067
Bst2UI CCWGG 1 cut(s) 648
Bst4CI ACNGT 2 cut(s) 53, 428
Bst6I CTCTTC 2 cut(s) 378, 495
BstC8I GCNNGC 4 cut(s) 386, 462, 692, 1086
BstDEI CTNAG 2 cut(s) 144, 489
BstF5I GGATG 6 cut(s) 212, 628, 710, 796, 882, 909
BstKTI GATC 4 cut(s) 129, 278, 298, 1003
BstMBI GATC 4 cut(s) 126, 275, 295, 1000
BstMCI CGRYCG 1 cut(s) 427
BstMWI GCNNNNNNNGC 3 cut(s) 69, 524, 1078
BstNI CCWGG 1 cut(s) 648
BstNSI RCATGY 2 cut(s) 589, 694
BstSCI CCNGG 2 cut(s) 409, 646
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 2 cut(s) 462, 562
BstV1I GCAGC 6 cut(s) 91, 410, 802, 908, 937, 1068
BstV2I GAAGAC 1 cut(s) 876
BstXI CCANNNNNNTGG 2 cut(s) 607, 900
Bsu15I ATCGAT 1 cut(s) 26
BsuRI GGCC 2 cut(s) 220, 518
BsuTUI ATCGAT 1 cut(s) 26
BtsCI GGATG 6 cut(s) 212, 628, 710, 796, 882, 909
BtsIMutI CAGTG 2 cut(s) 478, 821
Cac8I GCNNGC 4 cut(s) 386, 462, 692, 1086
CciI TCATGA 1 cut(s) 988
Cfr13I GGNCC 2 cut(s) 218, 911
ClaI ATCGAT 1 cut(s) 26
Csp6I GTAC 3 cut(s) 271, 407, 724
CviAII CATG 9 cut(s) 130, 259, 528, 586, 691, 718, 748, 900, 989
CviQI GTAC 3 cut(s) 271, 407, 724
DdeI CTNAG 2 cut(s) 144, 489
DpnI GATC 4 cut(s) 128, 277, 297, 1002
DpnII GATC 4 cut(s) 126, 275, 295, 1000
DraIII CACNNNGTG 1 cut(s) 457
Eam1104I CTCTTC 2 cut(s) 378, 495
EarI CTCTTC 2 cut(s) 378, 495
Eco130I CCWWGG 1 cut(s) 600
Eco147I AGGCCT 1 cut(s) 518
Eco32I GATATC 1 cut(s) 735
Eco47I GGWCC 1 cut(s) 911
EcoRII CCWGG 1 cut(s) 646
EcoRV GATATC 1 cut(s) 735
EcoT14I CCWWGG 1 cut(s) 600
EcoT22I ATGCAT 1 cut(s) 942
ErhI CCWWGG 1 cut(s) 600
FaeI CATG 9 cut(s) 133, 262, 531, 589, 694, 721, 751, 903, 992
FalI AAGNNNNNCTT 4 cut(s) 377, 409, 900, 932
FaqI GGGAC 1 cut(s) 574
FatI CATG 9 cut(s) 129, 258, 527, 585, 690, 717, 747, 899, 988
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 6 cut(s) 105, 399, 816, 897, 951, 1082
FokI GGATG 6 cut(s) 219, 635, 697, 803, 889, 916
Fsp4HI GCNGC 6 cut(s) 105, 399, 816, 897, 951, 1082
FspBI CTAG 2 cut(s) 251, 338
GluI GCNGC 6 cut(s) 105, 399, 816, 897, 951, 1082
GsaI CCCAGC 1 cut(s) 896
GsuI CTGGAG 3 cut(s) 507, 669, 845
HaeIII GGCC 2 cut(s) 220, 518
HapII CCGG 4 cut(s) 410, 437, 714, 972
Hin1II CATG 9 cut(s) 133, 262, 531, 589, 694, 721, 751, 903, 992
HindIII AAGCTT 2 cut(s) 149, 1028
HinfI GANTC 3 cut(s) 440, 544, 881
HpaII CCGG 4 cut(s) 410, 437, 714, 972
HphI GGTGA 3 cut(s) 148, 673, 946
Hpy166II GTNNAC 3 cut(s) 366, 460, 914
Hpy188I TCNGA 2 cut(s) 1036, 1045
Hpy188III TCNNGA 7 cut(s) 232, 290, 422, 437, 620, 675, 989
Hpy8I GTNNAC 3 cut(s) 366, 460, 914
HpyAV CCTTC 2 cut(s) 773, 953
HpyCH4III ACNGT 2 cut(s) 53, 428
HpyCH4IV ACGT 1 cut(s) 1015
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 524, 1078
HpyF3I CTNAG 2 cut(s) 144, 489
HpySE526I ACGT 1 cut(s) 1015
Hsp92II CATG 9 cut(s) 133, 262, 531, 589, 694, 721, 751, 903, 992
Kpn2I TCCGGA 1 cut(s) 436
KpnI GGTACC 1 cut(s) 410
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 4 cut(s) 126, 275, 295, 1000
LmnI GCTCC 1 cut(s) 292
Lsp1109I GCAGC 6 cut(s) 91, 410, 802, 908, 937, 1068
LweI GCATC 6 cut(s) 568, 613, 718, 719, 781, 927
MaeI CTAG 2 cut(s) 251, 338
MaeII ACGT 1 cut(s) 1015
MaeIII GTNAC 1 cut(s) 232
MalI GATC 4 cut(s) 128, 277, 297, 1002
MboI GATC 4 cut(s) 126, 275, 295, 1000
MboII GAAGA 4 cut(s) 319, 365, 512, 876
MhlI GDGCHC 2 cut(s) 462, 562
MluCI AATT 3 cut(s) 13, 314, 799
MlyI GAGTC 2 cut(s) 449, 890
MmeI TCCRAC 2 cut(s) 813, 1036
Mph1103I ATGCAT 1 cut(s) 942
MroI TCCGGA 1 cut(s) 436
MroXI GAANNNNTTC 1 cut(s) 350
MseI TTAA 1 cut(s) 849
MslI CAYNNNNRTG 3 cut(s) 134, 230, 752
MspA1I CMGCKG 1 cut(s) 1081
MspI CCGG 4 cut(s) 410, 437, 714, 972
MspR9I CCNGG 2 cut(s) 411, 648
MvaI CCWGG 1 cut(s) 648
MwoI GCNNNNNNNGC 3 cut(s) 69, 524, 1078
NciI CCSGG 1 cut(s) 411
NdeII GATC 4 cut(s) 126, 275, 295, 1000
NlaIII CATG 9 cut(s) 133, 262, 531, 589, 694, 721, 751, 903, 992
NlaIV GGNNCC 2 cut(s) 408, 1064
NmuCI GTSAC 1 cut(s) 232
NsiI ATGCAT 1 cut(s) 942
NspI RCATGY 2 cut(s) 589, 694
OliI CACNNNNGTG 1 cut(s) 230
PaeI GCATGC 1 cut(s) 694
PagI TCATGA 1 cut(s) 988
PceI AGGCCT 1 cut(s) 518
PciI ACATGT 1 cut(s) 585
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 544
PkrI GCNGC 6 cut(s) 106, 400, 817, 898, 952, 1083
PleI GAGTC 2 cut(s) 448, 889
PpsI GAGTC 2 cut(s) 448, 889
PscI ACATGT 1 cut(s) 585
PsiI TTATAA 1 cut(s) 1052
Psp1406I AACGTT 1 cut(s) 1015
Psp6I CCWGG 1 cut(s) 646
PspFI CCCAGC 1 cut(s) 892
PspGI CCWGG 1 cut(s) 646
PspN4I GGNNCC 2 cut(s) 408, 1064
PspPI GGNCC 2 cut(s) 218, 911
PsrI GAACNNNNNNTAC 2 cut(s) 74, 106
PstI CTGCAG 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 1081
RsaI GTAC 3 cut(s) 272, 408, 725
RsaNI GTAC 3 cut(s) 271, 407, 724
RseI CAYNNNNRTG 3 cut(s) 134, 230, 752
SaqAI TTAA 1 cut(s) 849
SatI GCNGC 6 cut(s) 105, 399, 816, 897, 951, 1082
Sau3AI GATC 4 cut(s) 126, 275, 295, 1000
Sau96I GGNCC 2 cut(s) 218, 911
SchI GAGTC 2 cut(s) 449, 890
ScrFI CCNGG 2 cut(s) 411, 648
SduI GDGCHC 2 cut(s) 462, 562
SfaNI GCATC 6 cut(s) 568, 613, 718, 719, 781, 927
SfcI CTRYAG 1 cut(s) 105
SinI GGWCC 1 cut(s) 911
SmiMI CAYNNNNRTG 3 cut(s) 134, 230, 752
SmlI CTYRAG 2 cut(s) 1057, 1094
SmoI CTYRAG 2 cut(s) 1057, 1094
SphI GCATGC 1 cut(s) 694
Sse9I AATT 3 cut(s) 13, 314, 799
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 1 cut(s) 708
SspMI CTAG 2 cut(s) 251, 338
StuI AGGCCT 1 cut(s) 518
StyD4I CCNGG 2 cut(s) 409, 646
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 2 cut(s) 53, 428
TaiI ACGT 1 cut(s) 1018
TaqI TCGA 2 cut(s) 26, 740
TasI AATT 3 cut(s) 13, 314, 799
TfiI GAWTC 1 cut(s) 544
Tru1I TTAA 1 cut(s) 849
Tru9I TTAA 1 cut(s) 849
TscAI CASTG 2 cut(s) 485, 828
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 6 cut(s) 104, 398, 815, 896, 950, 1081
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 6 cut(s) 17, 62, 173, 582, 792, 920
TspRI CASTG 2 cut(s) 485, 828
VneI GTGCAC 1 cut(s) 458
VpaK11BI GGWCC 1 cut(s) 911
XapI RAATTY 1 cut(s) 314
XceI RCATGY 2 cut(s) 589, 694
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 2 cut(s) 251, 338
Zsp2I ATGCAT 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.