Rh4BG407500

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008919
Physical Location & Seq
Forward (+)
55727220 .. 55729296
2077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG407500.1

Sequence Viewer

Length: 666 bp
ATGGCAAACTCAGTTCCCATGAATGGAAATAATTGCACAAAATCGATAACAGGTTCAATGCCAATGAACGGTGGAAACGGCACTCACAGCTACTCCAAGAACTCCTACTACCAGAAAAAAGCTGCAGAGATTGAAAAGGAAAAGATCATGGAGTTTGCCAAGGAAATGGAGAACTTTCTGGATGCGAGGGCTAAAGAGCTTGTGCCTGGAGGAATGATGGTGATGGTCTTGTCTGGAAATCATAAAGGCATGCCTTATTCTCAAACCCCATCCGGCATGATGTACGATGCGATATCATCGAGCCTCATGGATATGGAAAAAGAGGGATTGATTGATGAAGGTCAAGTGGATGCGTTCAACTTGCCTTTCTACGCTGCCTCACTGGAGGAGTTGGAGGGGCATGTGGTTAAAAATGGGTGCTTTAGCATCGAGAGGATGGAGTCTTCAAACCCAGCAGCATGGTTGAAAGGTCCAGTTGACATACCACAATTGGTGATGCATATACGGGCTGCAATGGAAGGAATGTTCGCCGGACACTTTGGAAGTCATGAGGTTATGGATCAAATGTTCCAACGTTCGGCTCAAAAGCTTTCAGACAAGTCTGAGCTTATAAACTCAAGGTGCCACGAGCAACGTCAGCTGCTTGCTGTTCTCAAGCGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

221

Amino Acids

24.56

Weight (kDa)

5.99

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 32 - 220 1.4e-41 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 611
AccB1I GGYRCC 1 cut(s) 621
AclI AACGTT 1 cut(s) 574
AclWI GGATC 1 cut(s) 567
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 3 cut(s) 23, 68, 577
AgsI TTSAA 5 cut(s) 57, 134, 358, 447, 466
AjnI CCWGG 1 cut(s) 205
AloI GAACNNNNNNTCC 4 cut(s) 509, 541, 551, 583
AluBI AGCT 6 cut(s) 90, 122, 199, 589, 607, 640
AluI AGCT 6 cut(s) 90, 122, 199, 589, 607, 640
AlwI GGATC 1 cut(s) 567
ApeKI GCWGC 5 cut(s) 122, 374, 455, 509, 640
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 2 cut(s) 232, 505
AvaII GGWCC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 621
BauI CACGAG 1 cut(s) 626
BbsI GAAGAC 1 cut(s) 435
BbvI GCAGC 5 cut(s) 109, 361, 467, 496, 627
BccI CCATC 4 cut(s) 211, 217, 277, 430
BceAI ACGGC 1 cut(s) 94
BciT130I CCWGG 1 cut(s) 207
BfmI CTRYAG 1 cut(s) 123
BisI GCNGC 5 cut(s) 123, 375, 456, 510, 641
BlsI GCNGC 5 cut(s) 124, 376, 457, 511, 642
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 1 cut(s) 623
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 5 cut(s) 172, 277, 340, 435, 486
BpiI GAAGAC 1 cut(s) 435
BpmI CTGGAG 2 cut(s) 228, 404
BpuEI CTTGAG 2 cut(s) 601, 638
Bsa29I ATCGAT 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 159
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 68, 577
Bse1I ACTGG 2 cut(s) 387, 473
Bse3DI GCAATG 1 cut(s) 519
BseBI CCWGG 1 cut(s) 207
BseCI ATCGAT 1 cut(s) 44
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 4 cut(s) 187, 269, 355, 441
BseLI CCNNNNNNNGG 3 cut(s) 23, 68, 577
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 2 cut(s) 24, 594
BseNI ACTGG 2 cut(s) 387, 473
BseRI GAGGAG 1 cut(s) 401
BseXI GCAGC 5 cut(s) 109, 361, 467, 496, 627
BseYI CCCAGC 1 cut(s) 451
BshNI GGYRCC 1 cut(s) 621
BshVI ATCGAT 1 cut(s) 44
BsiSI CCGG 2 cut(s) 273, 531
BslI CCNNNNNNNGG 3 cut(s) 23, 68, 577
Bsp143I GATC 2 cut(s) 144, 559
BspCNI CTCAG 2 cut(s) 23, 595
BspDI ATCGAT 1 cut(s) 44
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 1 cut(s) 623
BspMAI CTGCAG 1 cut(s) 127
BspPI GGATC 1 cut(s) 567
BspT107I GGYRCC 1 cut(s) 621
BsrDI GCAATG 1 cut(s) 519
BsrI ACTGG 2 cut(s) 387, 473
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 2 cut(s) 144, 559
BssSI CACGAG 1 cut(s) 626
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 626
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 71
BstC8I GCNNGC 2 cut(s) 251, 645
BstDEI CTNAG 2 cut(s) 10, 603
BstF5I GGATG 4 cut(s) 187, 269, 355, 441
BstKTI GATC 2 cut(s) 147, 562
BstMBI GATC 2 cut(s) 144, 559
BstMWI GCNNNNNNNGC 2 cut(s) 87, 637
BstNI CCWGG 1 cut(s) 207
BstNSI RCATGY 2 cut(s) 253, 404
BstSCI CCNGG 1 cut(s) 205
BstSFI CTRYAG 1 cut(s) 123
BstV1I GCAGC 5 cut(s) 109, 361, 467, 496, 627
BstV2I GAAGAC 1 cut(s) 435
BstXI CCANNNNNNTGG 2 cut(s) 166, 459
Bsu15I ATCGAT 1 cut(s) 44
BsuTUI ATCGAT 1 cut(s) 44
BtsCI GGATG 4 cut(s) 187, 269, 355, 441
BtsIMutI CAGTG 1 cut(s) 380
Cac8I GCNNGC 2 cut(s) 251, 645
CciI TCATGA 1 cut(s) 547
Cfr13I GGNCC 1 cut(s) 470
ClaI ATCGAT 1 cut(s) 44
Csp6I GTAC 1 cut(s) 283
CviAII CATG 8 cut(s) 19, 148, 250, 277, 307, 401, 459, 548
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 2 cut(s) 10, 603
DpnI GATC 2 cut(s) 146, 561
DpnII GATC 2 cut(s) 144, 559
Eco130I CCWWGG 1 cut(s) 159
Eco32I GATATC 1 cut(s) 294
Eco47I GGWCC 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 205
EcoRV GATATC 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 159
EcoT22I ATGCAT 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 8 cut(s) 22, 151, 253, 280, 310, 404, 462, 551
FatI CATG 8 cut(s) 18, 147, 249, 276, 306, 400, 458, 547
Fnu4HI GCNGC 5 cut(s) 123, 375, 456, 510, 641
FokI GGATG 4 cut(s) 194, 256, 362, 448
Fsp4HI GCNGC 5 cut(s) 123, 375, 456, 510, 641
GluI GCNGC 5 cut(s) 123, 375, 456, 510, 641
GsaI CCCAGC 1 cut(s) 455
GsuI CTGGAG 2 cut(s) 228, 404
HapII CCGG 2 cut(s) 273, 531
Hin1II CATG 8 cut(s) 22, 151, 253, 280, 310, 404, 462, 551
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 1 cut(s) 440
HpaII CCGG 2 cut(s) 273, 531
HphI GGTGA 2 cut(s) 232, 505
Hpy166II GTNNAC 1 cut(s) 478
Hpy188I TCNGA 2 cut(s) 595, 604
Hpy188III TCNNGA 4 cut(s) 179, 234, 430, 548
Hpy8I GTNNAC 1 cut(s) 478
HpyAV CCTTC 2 cut(s) 332, 512
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4IV ACGT 2 cut(s) 574, 634
HpyCH4V TGCA 4 cut(s) 36, 125, 499, 512
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 637
HpyF3I CTNAG 2 cut(s) 10, 603
HpySE526I ACGT 2 cut(s) 574, 634
Hsp92II CATG 8 cut(s) 22, 151, 253, 280, 310, 404, 462, 551
Kzo9I GATC 2 cut(s) 144, 559
Lsp1109I GCAGC 5 cut(s) 109, 361, 467, 496, 627
LweI GCATC 5 cut(s) 172, 277, 340, 435, 486
MaeII ACGT 2 cut(s) 574, 634
MalI GATC 2 cut(s) 146, 561
MboI GATC 2 cut(s) 144, 559
MboII GAAGA 1 cut(s) 435
MfeI CAATTG 1 cut(s) 488
MluCI AATT 2 cut(s) 31, 488
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 2 cut(s) 372, 595
MnlI CCTC 9 cut(s) 180, 203, 314, 316, 379, 388, 388, 426, 544
Mph1103I ATGCAT 1 cut(s) 501
MseI TTAA 1 cut(s) 408
MslI CAYNNNNRTG 1 cut(s) 311
MspA1I CMGCKG 1 cut(s) 640
MspI CCGG 2 cut(s) 273, 531
MspR9I CCNGG 1 cut(s) 207
MunI CAATTG 1 cut(s) 488
MvaI CCWGG 1 cut(s) 207
MwoI GCNNNNNNNGC 2 cut(s) 87, 637
NdeII GATC 2 cut(s) 144, 559
NlaIII CATG 8 cut(s) 22, 151, 253, 280, 310, 404, 462, 551
NlaIV GGNNCC 1 cut(s) 623
NsiI ATGCAT 1 cut(s) 501
NspI RCATGY 2 cut(s) 253, 404
PaeI GCATGC 1 cut(s) 253
PagI TCATGA 1 cut(s) 547
PkrI GCNGC 5 cut(s) 124, 376, 457, 511, 642
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PsiI TTATAA 1 cut(s) 611
Psp1406I AACGTT 1 cut(s) 574
Psp6I CCWGG 1 cut(s) 205
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 623
PspPI GGNCC 1 cut(s) 470
PsrI GAACNNNNNNTAC 2 cut(s) 92, 124
PstI CTGCAG 1 cut(s) 127
PvuII CAGCTG 1 cut(s) 640
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
RseI CAYNNNNRTG 1 cut(s) 311
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 5 cut(s) 123, 375, 456, 510, 641
Sau3AI GATC 2 cut(s) 144, 559
Sau96I GGNCC 1 cut(s) 470
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 1 cut(s) 207
SfaNI GCATC 5 cut(s) 172, 277, 340, 435, 486
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 1 cut(s) 311
SmlI CTYRAG 2 cut(s) 616, 653
SmoI CTYRAG 2 cut(s) 616, 653
SphI GCATGC 1 cut(s) 253
Sse9I AATT 2 cut(s) 31, 488
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 1 cut(s) 71
TaiI ACGT 2 cut(s) 577, 637
TaqI TCGA 3 cut(s) 44, 299, 429
TasI AATT 2 cut(s) 31, 488
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 1 cut(s) 387
TseI GCWGC 5 cut(s) 122, 374, 455, 509, 640
TspDTI ATGAA 3 cut(s) 35, 80, 351
TspRI CASTG 1 cut(s) 387
VpaK11BI GGWCC 1 cut(s) 470
XceI RCATGY 2 cut(s) 253, 404
Zsp2I ATGCAT 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.