RLG00000005993

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008919
Physical Location & Seq
Reverse (-)
3359550 .. 3362064
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005993

Sequence Viewer

Length: 1107 bp
ATGAATGGAAATAATTGCACGAAATCGATAACAGGTTCAATGCCAATGAACGGTGGAAACGGCACTCACAGCTACTCCAAGAACTCCTACTACCAGAAAAAAGCTGCAGAGATTGAAAAGGAAAAGATCATGGAGGTGATTGCTGAGAAGCTTGATATGAAAAACCTCTCCTCAATCTCAAACACTATTCGTATTGCAGACTTGGGATGTTCAACTGGGCCAAACACTTTCGTGACAATGCAAAACATAATAGAAGCCATGCAACAGAAGTACCGATCCCAATCTGCTCCTGATGATCATCAAACAATGCCTGAATTTCAAGTGTTCTTCAACGACCTAGTGTTGAATGACTTCAATACCCTGTTTACCTCACTTCCCAAAGACAAGCAATACTTTGCAGCAGGGGTACCGGGGTCTTTTCACGACCGTCTGTTTCCAGAGTCCTCTATCCACCTTGTGCACGCCTCACTTTCCCTCCACTGGCTCTCTAAGTTGCCAGAAGAGTTGCAAGACAGGGCCTCTCCAGCATGGAACAAAGGGAGGATTCACTACACAAGTGCCCCTAATGAAGTGGTTGATGCTTACATGTCCCAGTTTGCCAAGGAAATGGAGAACTTTCTGGATGCGAGGGCTAAAGAGCTTGTGCCTGGAGGAATGATGGTGATGGTCTTGTCTGGAAATCACAAAGGCATGCCTTATTCTCAAACCCCATCCGGCATGATGTACGATGCGATATCATCCAGCCTCATGGATATGGAAAAAGAGGGATTGATTGATGAAGGTCAAGTGGATGCTTTCAACTTGCCTTTCTACGCTGCCTCACTGGAGGAGTTGGAGGGGCTTGTGGTTAAAAATGGGTGCTTTAGCATCGAGAGGATGGAGTCTTCAAACCCAGCAGCATGGATGAAAGGTCCAGTTGACATACCACAATGGGTGATGCATATACGGGCTGCAATGGAAGGAATGTTCGCCGGACATTTTGGAAGTCATGAGGTTATGGATCAAATGTTCCAACGTTCGGCTCAAAAGCTTTCAGACGAGTCTGAGCTTATAAACTCAAGGTGCCAGGAGCAAAGTCAGCTTCTTGCTGTTCTCAAGCGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

369

Amino Acids

41.22

Weight (kDa)

5.42

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 56 - 367 8.3e-108 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1052
Acc65I GGTACC 1 cut(s) 406
AccB1I GGYRCC 2 cut(s) 406, 1062
AclI AACGTT 1 cut(s) 1015
AclWI GGATC 2 cut(s) 270, 1008
AcsI RAATTY 1 cut(s) 314
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 3 cut(s) 272, 408, 725
AfiI CCNNNNNNNGG 3 cut(s) 50, 480, 1018
AflIII ACRYGT 1 cut(s) 585
AgsI TTSAA 9 cut(s) 39, 116, 213, 320, 331, 346, 355, 799, 888
AjnI CCWGG 2 cut(s) 646, 1065
AleI CACNNNNGTG 1 cut(s) 230
AloI GAACNNNNNNTCC 4 cut(s) 950, 982, 992, 1024
AluBI AGCT 7 cut(s) 72, 104, 151, 640, 1030, 1048, 1081
AluI AGCT 7 cut(s) 72, 104, 151, 640, 1030, 1048, 1081
Alw21I GWGCWC 1 cut(s) 462
Alw44I GTGCAC 1 cut(s) 458
AlwI GGATC 2 cut(s) 270, 1008
AoxI GGCC 2 cut(s) 218, 516
ApaLI GTGCAC 1 cut(s) 458
ApeKI GCWGC 5 cut(s) 104, 398, 815, 896, 950
ApoI RAATTY 1 cut(s) 314
Asp700I GAANNNNTTC 1 cut(s) 350
Asp718I GGTACC 1 cut(s) 406
AspS9I GGNCC 3 cut(s) 218, 516, 911
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 3 cut(s) 148, 673, 946
AvaII GGWCC 1 cut(s) 911
BaeGI GKGCMC 2 cut(s) 462, 562
BaeI ACNNNNGTAYC 4 cut(s) 254, 287, 398, 431
BanI GGYRCC 2 cut(s) 406, 1062
BbsI GAAGAC 1 cut(s) 876
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 5 cut(s) 91, 410, 802, 908, 937
BccI CCATC 4 cut(s) 652, 658, 718, 871
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 2 cut(s) 648, 1067
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 1 cut(s) 411
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 1 cut(s) 105
BisI GCNGC 5 cut(s) 105, 399, 816, 897, 951
BlsI GCNGC 5 cut(s) 106, 400, 817, 898, 952
Bme1390I CCNGG 3 cut(s) 411, 648, 1067
Bme18I GGWCC 1 cut(s) 911
BmgT120I GGNCC 3 cut(s) 218, 516, 911
BmiI GGNNCC 2 cut(s) 408, 1064
BmrFI CCNGG 3 cut(s) 411, 648, 1067
BmrI ACTGGG 2 cut(s) 225, 586
BmsI GCATC 6 cut(s) 568, 613, 718, 781, 876, 927
BmuI ACTGGG 2 cut(s) 225, 586
BpiI GAAGAC 1 cut(s) 876
BpmI CTGGAG 3 cut(s) 507, 669, 845
BpuEI CTTGAG 2 cut(s) 1042, 1079
BpuMI CCSGG 1 cut(s) 411
Bsa29I ATCGAT 1 cut(s) 26
BsaBI GATNNNNATC 2 cut(s) 280, 297
BsaJI CCNNGG 2 cut(s) 410, 600
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 459, 489
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 480, 1018
Bse1I ACTGG 5 cut(s) 220, 485, 592, 828, 914
Bse3DI GCAATG 1 cut(s) 960
Bse8I GATNNNNATC 2 cut(s) 280, 297
BseBI CCWGG 2 cut(s) 648, 1067
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 410, 600
BseGI GGATG 7 cut(s) 212, 628, 710, 737, 796, 882, 909
BseJI GATNNNNATC 2 cut(s) 280, 297
BseLI CCNNNNNNNGG 3 cut(s) 50, 480, 1018
BseMI GCAATG 1 cut(s) 960
BseMII CTCAG 2 cut(s) 135, 1035
BseNI ACTGG 5 cut(s) 220, 485, 592, 828, 914
BseRI GAGGAG 2 cut(s) 160, 842
BseSI GKGCMC 2 cut(s) 462, 562
BseXI GCAGC 5 cut(s) 91, 410, 802, 908, 937
BseYI CCCAGC 1 cut(s) 892
Bsh1285I CGRYCG 1 cut(s) 427
BshFI GGCC 2 cut(s) 220, 518
BshNI GGYRCC 2 cut(s) 406, 1062
BshVI ATCGAT 1 cut(s) 26
BsiEI CGRYCG 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 462
BsiSI CCGG 3 cut(s) 410, 714, 972
BslFI GGGAC 1 cut(s) 574
BslI CCNNNNNNNGG 3 cut(s) 50, 480, 1018
BsmFI GGGAC 1 cut(s) 574
BsnI GGCC 2 cut(s) 220, 518
Bsp1286I GDGCHC 2 cut(s) 462, 562
Bsp143I GATC 4 cut(s) 126, 275, 295, 1000
BspANI GGCC 2 cut(s) 220, 518
BspCNI CTCAG 2 cut(s) 136, 1036
BspDI ATCGAT 1 cut(s) 26
BspHI TCATGA 1 cut(s) 988
BspLI GGNNCC 2 cut(s) 408, 1064
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 2 cut(s) 270, 1008
BspT107I GGYRCC 2 cut(s) 406, 1062
BsrDI GCAATG 1 cut(s) 960
BsrI ACTGG 5 cut(s) 220, 485, 592, 828, 914
BssECI CCNNGG 2 cut(s) 410, 600
BssMI GATC 4 cut(s) 126, 275, 295, 1000
BssT1I CCWWGG 1 cut(s) 600
Bst2UI CCWGG 2 cut(s) 648, 1067
Bst4CI ACNGT 2 cut(s) 53, 428
Bst6I CTCTTC 1 cut(s) 495
BstC8I GCNNGC 2 cut(s) 462, 692
BstDEI CTNAG 3 cut(s) 144, 489, 1044
BstF5I GGATG 7 cut(s) 212, 628, 710, 737, 796, 882, 909
BstKTI GATC 4 cut(s) 129, 278, 298, 1003
BstMBI GATC 4 cut(s) 126, 275, 295, 1000
BstMCI CGRYCG 1 cut(s) 427
BstMWI GCNNNNNNNGC 3 cut(s) 69, 524, 1078
BstNI CCWGG 2 cut(s) 648, 1067
BstNSI RCATGY 2 cut(s) 589, 694
BstSCI CCNGG 3 cut(s) 409, 646, 1065
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 2 cut(s) 462, 562
BstV1I GCAGC 5 cut(s) 91, 410, 802, 908, 937
BstV2I GAAGAC 1 cut(s) 876
BstXI CCANNNNNNTGG 3 cut(s) 607, 748, 900
Bsu15I ATCGAT 1 cut(s) 26
BsuRI GGCC 2 cut(s) 220, 518
BsuTUI ATCGAT 1 cut(s) 26
BtsCI GGATG 7 cut(s) 212, 628, 710, 737, 796, 882, 909
BtsIMutI CAGTG 2 cut(s) 478, 821
Cac8I GCNNGC 2 cut(s) 462, 692
CciI TCATGA 1 cut(s) 988
Cfr13I GGNCC 3 cut(s) 218, 516, 911
ClaI ATCGAT 1 cut(s) 26
Csp6I GTAC 3 cut(s) 271, 407, 724
CviAII CATG 9 cut(s) 130, 259, 528, 586, 691, 718, 748, 900, 989
CviQI GTAC 3 cut(s) 271, 407, 724
DdeI CTNAG 3 cut(s) 144, 489, 1044
DpnI GATC 4 cut(s) 128, 277, 297, 1002
DpnII GATC 4 cut(s) 126, 275, 295, 1000
DraIII CACNNNGTG 1 cut(s) 457
Eam1104I CTCTTC 1 cut(s) 495
EarI CTCTTC 1 cut(s) 495
Eco130I CCWWGG 1 cut(s) 600
Eco32I GATATC 1 cut(s) 735
Eco47I GGWCC 1 cut(s) 911
EcoO109I RGGNCCY 1 cut(s) 516
EcoRII CCWGG 2 cut(s) 646, 1065
EcoRV GATATC 1 cut(s) 735
EcoT14I CCWWGG 1 cut(s) 600
EcoT22I ATGCAT 1 cut(s) 942
ErhI CCWWGG 1 cut(s) 600
FaeI CATG 9 cut(s) 133, 262, 531, 589, 694, 721, 751, 903, 992
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FaqI GGGAC 1 cut(s) 574
FatI CATG 9 cut(s) 129, 258, 527, 585, 690, 717, 747, 899, 988
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 5 cut(s) 105, 399, 816, 897, 951
FokI GGATG 7 cut(s) 219, 635, 697, 724, 803, 889, 916
Fsp4HI GCNGC 5 cut(s) 105, 399, 816, 897, 951
FspBI CTAG 1 cut(s) 338
GluI GCNGC 5 cut(s) 105, 399, 816, 897, 951
GsaI CCCAGC 1 cut(s) 896
GsuI CTGGAG 3 cut(s) 507, 669, 845
HaeIII GGCC 2 cut(s) 220, 518
HapII CCGG 3 cut(s) 410, 714, 972
Hin1II CATG 9 cut(s) 133, 262, 531, 589, 694, 721, 751, 903, 992
HincII GTYRAC 1 cut(s) 919
HindII GTYRAC 1 cut(s) 919
HindIII AAGCTT 2 cut(s) 149, 1028
HinfI GANTC 4 cut(s) 440, 544, 881, 1040
HpaII CCGG 3 cut(s) 410, 714, 972
HphI GGTGA 3 cut(s) 148, 673, 946
Hpy166II GTNNAC 3 cut(s) 366, 460, 919
Hpy188I TCNGA 2 cut(s) 1036, 1045
Hpy188III TCNNGA 8 cut(s) 232, 290, 422, 437, 620, 675, 871, 989
Hpy8I GTNNAC 3 cut(s) 366, 460, 919
HpyAV CCTTC 2 cut(s) 773, 953
HpyCH4III ACNGT 2 cut(s) 53, 428
HpyCH4IV ACGT 1 cut(s) 1015
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 524, 1078
HpyF3I CTNAG 3 cut(s) 144, 489, 1044
HpySE526I ACGT 1 cut(s) 1015
Hsp92II CATG 9 cut(s) 133, 262, 531, 589, 694, 721, 751, 903, 992
KpnI GGTACC 1 cut(s) 410
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 4 cut(s) 126, 275, 295, 1000
LmnI GCTCC 2 cut(s) 292, 1069
Lsp1109I GCAGC 5 cut(s) 91, 410, 802, 908, 937
LweI GCATC 6 cut(s) 568, 613, 718, 781, 876, 927
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 1015
MaeIII GTNAC 1 cut(s) 232
MalI GATC 4 cut(s) 128, 277, 297, 1002
MboI GATC 4 cut(s) 126, 275, 295, 1000
MboII GAAGA 3 cut(s) 319, 512, 876
MhlI GDGCHC 2 cut(s) 462, 562
MluCI AATT 2 cut(s) 13, 314
MlyI GAGTC 3 cut(s) 449, 890, 1049
MmeI TCCRAC 2 cut(s) 813, 1036
Mph1103I ATGCAT 1 cut(s) 942
MroXI GAANNNNTTC 1 cut(s) 350
MseI TTAA 1 cut(s) 849
MslI CAYNNNNRTG 3 cut(s) 134, 230, 752
MspI CCGG 3 cut(s) 410, 714, 972
MspR9I CCNGG 3 cut(s) 411, 648, 1067
MvaI CCWGG 2 cut(s) 648, 1067
MwoI GCNNNNNNNGC 3 cut(s) 69, 524, 1078
NciI CCSGG 1 cut(s) 411
NdeII GATC 4 cut(s) 126, 275, 295, 1000
NlaIII CATG 9 cut(s) 133, 262, 531, 589, 694, 721, 751, 903, 992
NlaIV GGNNCC 2 cut(s) 408, 1064
NmuCI GTSAC 1 cut(s) 232
NsiI ATGCAT 1 cut(s) 942
NspI RCATGY 2 cut(s) 589, 694
OliI CACNNNNGTG 1 cut(s) 230
PaeI GCATGC 1 cut(s) 694
PagI TCATGA 1 cut(s) 988
PciI ACATGT 1 cut(s) 585
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 544
PkrI GCNGC 5 cut(s) 106, 400, 817, 898, 952
PleI GAGTC 3 cut(s) 448, 889, 1048
PpsI GAGTC 3 cut(s) 448, 889, 1048
PscI ACATGT 1 cut(s) 585
PsiI TTATAA 1 cut(s) 1052
Psp1406I AACGTT 1 cut(s) 1015
Psp6I CCWGG 2 cut(s) 646, 1065
PspFI CCCAGC 1 cut(s) 892
PspGI CCWGG 2 cut(s) 646, 1065
PspN4I GGNNCC 2 cut(s) 408, 1064
PspPI GGNCC 3 cut(s) 218, 516, 911
PsrI GAACNNNNNNTAC 2 cut(s) 74, 106
PstI CTGCAG 1 cut(s) 109
RsaI GTAC 3 cut(s) 272, 408, 725
RsaNI GTAC 3 cut(s) 271, 407, 724
RseI CAYNNNNRTG 3 cut(s) 134, 230, 752
SaqAI TTAA 1 cut(s) 849
SatI GCNGC 5 cut(s) 105, 399, 816, 897, 951
Sau3AI GATC 4 cut(s) 126, 275, 295, 1000
Sau96I GGNCC 3 cut(s) 218, 516, 911
SchI GAGTC 3 cut(s) 449, 890, 1049
ScrFI CCNGG 3 cut(s) 411, 648, 1067
SduI GDGCHC 2 cut(s) 462, 562
SfaNI GCATC 6 cut(s) 568, 613, 718, 781, 876, 927
SfcI CTRYAG 1 cut(s) 105
SinI GGWCC 1 cut(s) 911
SmiMI CAYNNNNRTG 3 cut(s) 134, 230, 752
SmlI CTYRAG 2 cut(s) 1057, 1094
SmoI CTYRAG 2 cut(s) 1057, 1094
SphI GCATGC 1 cut(s) 694
Sse9I AATT 2 cut(s) 13, 314
SspMI CTAG 1 cut(s) 338
StyD4I CCNGG 3 cut(s) 409, 646, 1065
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 2 cut(s) 53, 428
TaiI ACGT 1 cut(s) 1018
TaqI TCGA 2 cut(s) 26, 870
TasI AATT 2 cut(s) 13, 314
TfiI GAWTC 1 cut(s) 544
Tru1I TTAA 1 cut(s) 849
Tru9I TTAA 1 cut(s) 849
TscAI CASTG 2 cut(s) 485, 828
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 5 cut(s) 104, 398, 815, 896, 950
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 6 cut(s) 17, 62, 173, 582, 792, 920
TspRI CASTG 2 cut(s) 485, 828
VneI GTGCAC 1 cut(s) 458
VpaK11BI GGWCC 1 cut(s) 911
XapI RAATTY 1 cut(s) 314
XceI RCATGY 2 cut(s) 589, 694
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 1 cut(s) 338
Zsp2I ATGCAT 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.