Rh4CG422900

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008919
Physical Location & Seq
Forward (+)
66628701 .. 66631058
2358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG422900.1

Sequence Viewer

Length: 1125 bp
ATGGCAAACTCAGTTCCCATGAATGGAAATAATTGCACAAAATCGATAACAGGTTCAATGCCAATGAACGGTGGAAACGGCACTCACAGCTACTCCAAGAACTCCTACTACCAGAAAAAAGCTGCAGAGATTGAAAAGGAAAAGATCATGGAGGTGATTGCTGAGAAGCTTGATATGAAAAACCTCTCCTCAATGTCAAACACTATTCGTATTGCAGACTTGGGATGTTCAACTGGGCCAAACACTTTCGTGACAATGCAAAACCTACTAGAAGCCACGCAACAAAAGTACCGATCCCAATCTGCTCCTGATGATCATCAAACAATGCCTGAATTTCAAGTGTTCTTCAACGACCTAGTGTTGAATGACTTCAATACCCTGTTTACCTCTCTTCCCAAAGACAAGCAATACTTTGCAGCAGGGGTACCGGGGTCTTTTCACGACCGTCTGTTTCCGGAGTCCTCTATCCACCTTGTACACGCCTCACTTTCCCTCCACTGGCTCTCTAAGTTGCCAGAAGAGTTGCAAGACAAGGCCTCTCCAGCATGGAACAAAGGGAGGATTCACTACACAAGTGCCCCTAATGAAGTGGTTGATGCTTACATGTCCCAGTTTGCCAAGGAAATGGAGAACTTTCTGGATGCGAGGGCTAAAGAGCTTGTGCCTGGAGGAATGATGGTGATGGTCTTGTCTGGAAATCATAAAGGCATGCCTTATTCTCAAACCCCATCCGGCATGATGTACGATGCGATATCATCGAGCCTCATGGATATGGAAAAAGAGGGATTGATTGATGAAGGTCAAGTGGATGCGTTCAACTTGCCTTTCTACGCTGCCTCACTGGAGGAGTTGGAGGGGCATGTGGTTAAAAATGGGTGCTTTAGCATCGAGAGGATGGAGTCTTCAAACCCAGCAGCATGGTTGAAAGGTCCAGTTGACATACCACAATTGGTGATGCATATACGGGCTGCAATGGAAGGAATGTTCGCCGGACACTTTGGAAGTCATGAGGTTATGGATCAAATGTTCCAACGTTCGGCTCAAAAGCTTTCAGACAAGTCTGAGCTTATAAACTCAAGGTGCCACGAGCAACGTCAGCTGCTTGCTGTTCTCAAGCGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

374

Amino Acids

41.79

Weight (kDa)

5.82

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 61 - 373 2.4e-105 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1070
Acc65I GGTACC 1 cut(s) 424
AccB1I GGYRCC 2 cut(s) 424, 1080
AccIII TCCGGA 1 cut(s) 454
AclI AACGTT 1 cut(s) 1033
AclWI GGATC 2 cut(s) 288, 1026
AcsI RAATTY 1 cut(s) 332
AfaI GTAC 4 cut(s) 290, 426, 477, 743
AfiI CCNNNNNNNGG 4 cut(s) 23, 68, 498, 1036
AflIII ACRYGT 1 cut(s) 603
AjnI CCWGG 1 cut(s) 664
AleI CACNNNNGTG 1 cut(s) 248
AloI GAACNNNNNNTCC 4 cut(s) 968, 1000, 1010, 1042
AluBI AGCT 7 cut(s) 90, 122, 169, 658, 1048, 1066, 1099
AluI AGCT 7 cut(s) 90, 122, 169, 658, 1048, 1066, 1099
AlwI GGATC 2 cut(s) 288, 1026
Aor13HI TCCGGA 1 cut(s) 454
AoxI GGCC 2 cut(s) 236, 534
ApeKI GCWGC 6 cut(s) 122, 416, 833, 914, 968, 1099
ApoI RAATTY 1 cut(s) 332
Asp700I GAANNNNTTC 1 cut(s) 368
Asp718I GGTACC 1 cut(s) 424
AspS9I GGNCC 2 cut(s) 236, 929
AsuC2I CCSGG 1 cut(s) 429
AsuHPI GGTGA 3 cut(s) 166, 691, 964
AvaII GGWCC 1 cut(s) 929
BaeGI GKGCMC 1 cut(s) 580
BaeI ACNNNNGTAYC 4 cut(s) 272, 305, 416, 449
BanI GGYRCC 2 cut(s) 424, 1080
BauI CACGAG 1 cut(s) 1085
BbsI GAAGAC 1 cut(s) 894
BbvI GCAGC 6 cut(s) 109, 428, 820, 926, 955, 1086
BccI CCATC 4 cut(s) 670, 676, 736, 889
BceAI ACGGC 1 cut(s) 94
BciT130I CCWGG 1 cut(s) 666
BclI TGATCA 1 cut(s) 313
BcnI CCSGG 1 cut(s) 429
BfaI CTAG 2 cut(s) 269, 356
BfmI CTRYAG 1 cut(s) 123
BisI GCNGC 6 cut(s) 123, 417, 834, 915, 969, 1100
BlsI GCNGC 6 cut(s) 124, 418, 835, 916, 970, 1101
Bme1390I CCNGG 2 cut(s) 429, 666
Bme18I GGWCC 1 cut(s) 929
BmgT120I GGNCC 2 cut(s) 236, 929
BmiI GGNNCC 2 cut(s) 426, 1082
BmrFI CCNGG 2 cut(s) 429, 666
BmrI ACTGGG 2 cut(s) 243, 604
BmsI GCATC 6 cut(s) 586, 631, 736, 799, 894, 945
BmuI ACTGGG 2 cut(s) 243, 604
BpiI GAAGAC 1 cut(s) 894
BpmI CTGGAG 3 cut(s) 525, 687, 863
BpuEI CTTGAG 2 cut(s) 1060, 1097
BpuMI CCSGG 1 cut(s) 429
Bsa29I ATCGAT 1 cut(s) 44
BsaBI GATNNNNATC 2 cut(s) 298, 315
BsaJI CCNNGG 2 cut(s) 428, 618
BsaWI WCCGGW 1 cut(s) 454
BsaXI ACNNNNNCTCC 4 cut(s) 77, 107, 477, 507
Bsc4I CCNNNNNNNGG 4 cut(s) 23, 68, 498, 1036
Bse1I ACTGG 5 cut(s) 238, 503, 610, 846, 932
Bse3DI GCAATG 1 cut(s) 978
Bse8I GATNNNNATC 2 cut(s) 298, 315
BseAI TCCGGA 1 cut(s) 454
BseBI CCWGG 1 cut(s) 666
BseCI ATCGAT 1 cut(s) 44
BseDI CCNNGG 2 cut(s) 428, 618
BseGI GGATG 5 cut(s) 230, 646, 728, 814, 900
BseJI GATNNNNATC 2 cut(s) 298, 315
BseLI CCNNNNNNNGG 4 cut(s) 23, 68, 498, 1036
BseMI GCAATG 1 cut(s) 978
BseMII CTCAG 3 cut(s) 24, 153, 1053
BseNI ACTGG 5 cut(s) 238, 503, 610, 846, 932
BseRI GAGGAG 2 cut(s) 178, 860
BseSI GKGCMC 1 cut(s) 580
BseXI GCAGC 6 cut(s) 109, 428, 820, 926, 955, 1086
BseYI CCCAGC 1 cut(s) 910
Bsh1285I CGRYCG 1 cut(s) 445
BshFI GGCC 2 cut(s) 238, 536
BshNI GGYRCC 2 cut(s) 424, 1080
BshVI ATCGAT 1 cut(s) 44
BsiEI CGRYCG 1 cut(s) 445
BsiSI CCGG 4 cut(s) 428, 455, 732, 990
BslFI GGGAC 1 cut(s) 592
BslI CCNNNNNNNGG 4 cut(s) 23, 68, 498, 1036
BsmFI GGGAC 1 cut(s) 592
BsnI GGCC 2 cut(s) 238, 536
Bsp1286I GDGCHC 1 cut(s) 580
Bsp13I TCCGGA 1 cut(s) 454
Bsp1407I TGTACA 1 cut(s) 475
Bsp143I GATC 4 cut(s) 144, 293, 313, 1018
BspANI GGCC 2 cut(s) 238, 536
BspCNI CTCAG 3 cut(s) 23, 154, 1054
BspDI ATCGAT 1 cut(s) 44
BspEI TCCGGA 1 cut(s) 454
BspHI TCATGA 1 cut(s) 1006
BspLI GGNNCC 2 cut(s) 426, 1082
BspMAI CTGCAG 1 cut(s) 127
BspPI GGATC 2 cut(s) 288, 1026
BspT107I GGYRCC 2 cut(s) 424, 1080
BsrDI GCAATG 1 cut(s) 978
BsrGI TGTACA 1 cut(s) 475
BsrI ACTGG 5 cut(s) 238, 503, 610, 846, 932
BssECI CCNNGG 2 cut(s) 428, 618
BssMI GATC 4 cut(s) 144, 293, 313, 1018
BssSI CACGAG 1 cut(s) 1085
BssT1I CCWWGG 1 cut(s) 618
Bst2BI CACGAG 1 cut(s) 1085
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 2 cut(s) 71, 446
Bst6I CTCTTC 2 cut(s) 396, 513
BstAUI TGTACA 1 cut(s) 475
BstC8I GCNNGC 2 cut(s) 710, 1104
BstDEI CTNAG 4 cut(s) 10, 162, 507, 1062
BstF5I GGATG 5 cut(s) 230, 646, 728, 814, 900
BstKTI GATC 4 cut(s) 147, 296, 316, 1021
BstMBI GATC 4 cut(s) 144, 293, 313, 1018
BstMCI CGRYCG 1 cut(s) 445
BstMWI GCNNNNNNNGC 3 cut(s) 87, 542, 1096
BstNI CCWGG 1 cut(s) 666
BstNSI RCATGY 3 cut(s) 607, 712, 863
BstSCI CCNGG 2 cut(s) 427, 664
BstSFI CTRYAG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 580
BstV1I GCAGC 6 cut(s) 109, 428, 820, 926, 955, 1086
BstV2I GAAGAC 1 cut(s) 894
BstXI CCANNNNNNTGG 2 cut(s) 625, 918
Bsu15I ATCGAT 1 cut(s) 44
BsuRI GGCC 2 cut(s) 238, 536
BsuTUI ATCGAT 1 cut(s) 44
BtsCI GGATG 5 cut(s) 230, 646, 728, 814, 900
BtsIMutI CAGTG 2 cut(s) 496, 839
Cac8I GCNNGC 2 cut(s) 710, 1104
CciI TCATGA 1 cut(s) 1006
Cfr13I GGNCC 2 cut(s) 236, 929
ClaI ATCGAT 1 cut(s) 44
Csp6I GTAC 4 cut(s) 289, 425, 476, 742
CviQI GTAC 4 cut(s) 289, 425, 476, 742
DdeI CTNAG 4 cut(s) 10, 162, 507, 1062
DpnI GATC 4 cut(s) 146, 295, 315, 1020
DpnII GATC 4 cut(s) 144, 293, 313, 1018
Eam1104I CTCTTC 2 cut(s) 396, 513
EarI CTCTTC 2 cut(s) 396, 513
Eco130I CCWWGG 1 cut(s) 618
Eco147I AGGCCT 1 cut(s) 536
Eco32I GATATC 1 cut(s) 753
Eco47I GGWCC 1 cut(s) 929
EcoRII CCWGG 1 cut(s) 664
EcoRV GATATC 1 cut(s) 753
EcoT14I CCWWGG 1 cut(s) 618
EcoT22I ATGCAT 1 cut(s) 960
ErhI CCWWGG 1 cut(s) 618
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 1 cut(s) 592
FbaI TGATCA 1 cut(s) 313
Fnu4HI GCNGC 6 cut(s) 123, 417, 834, 915, 969, 1100
FokI GGATG 5 cut(s) 237, 653, 715, 821, 907
Fsp4HI GCNGC 6 cut(s) 123, 417, 834, 915, 969, 1100
FspBI CTAG 2 cut(s) 269, 356
GluI GCNGC 6 cut(s) 123, 417, 834, 915, 969, 1100
GsaI CCCAGC 1 cut(s) 914
GsuI CTGGAG 3 cut(s) 525, 687, 863
HaeIII GGCC 2 cut(s) 238, 536
HapII CCGG 4 cut(s) 428, 455, 732, 990
HincII GTYRAC 1 cut(s) 937
HindII GTYRAC 1 cut(s) 937
HindIII AAGCTT 2 cut(s) 167, 1046
HinfI GANTC 3 cut(s) 458, 562, 899
HpaII CCGG 4 cut(s) 428, 455, 732, 990
HphI GGTGA 3 cut(s) 166, 691, 964
Hpy166II GTNNAC 3 cut(s) 384, 478, 937
Hpy188I TCNGA 2 cut(s) 1054, 1063
Hpy188III TCNNGA 8 cut(s) 250, 308, 440, 455, 638, 693, 889, 1007
Hpy8I GTNNAC 3 cut(s) 384, 478, 937
HpyAV CCTTC 2 cut(s) 791, 971
HpyCH4III ACNGT 2 cut(s) 71, 446
HpyCH4IV ACGT 2 cut(s) 1033, 1093
HpyCH4V TGCA 8 cut(s) 36, 125, 215, 259, 416, 526, 958, 971
HpyF10VI GCNNNNNNNGC 3 cut(s) 87, 542, 1096
HpyF3I CTNAG 4 cut(s) 10, 162, 507, 1062
HpySE526I ACGT 2 cut(s) 1033, 1093
Kpn2I TCCGGA 1 cut(s) 454
KpnI GGTACC 1 cut(s) 428
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 4 cut(s) 144, 293, 313, 1018
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 6 cut(s) 109, 428, 820, 926, 955, 1086
LweI GCATC 6 cut(s) 586, 631, 736, 799, 894, 945
MaeI CTAG 2 cut(s) 269, 356
MaeII ACGT 2 cut(s) 1033, 1093
MaeIII GTNAC 1 cut(s) 250
MalI GATC 4 cut(s) 146, 295, 315, 1020
MboI GATC 4 cut(s) 144, 293, 313, 1018
MboII GAAGA 4 cut(s) 337, 383, 530, 894
MfeI CAATTG 1 cut(s) 947
MhlI GDGCHC 1 cut(s) 580
MluCI AATT 3 cut(s) 31, 332, 947
MlyI GAGTC 2 cut(s) 467, 908
MmeI TCCRAC 2 cut(s) 831, 1054
Mph1103I ATGCAT 1 cut(s) 960
MroI TCCGGA 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 368
MseI TTAA 1 cut(s) 867
MslI CAYNNNNRTG 3 cut(s) 152, 248, 770
MspA1I CMGCKG 1 cut(s) 1099
MspI CCGG 4 cut(s) 428, 455, 732, 990
MspR9I CCNGG 2 cut(s) 429, 666
MunI CAATTG 1 cut(s) 947
MvaI CCWGG 1 cut(s) 666
MwoI GCNNNNNNNGC 3 cut(s) 87, 542, 1096
NciI CCSGG 1 cut(s) 429
NdeII GATC 4 cut(s) 144, 293, 313, 1018
NlaIV GGNNCC 2 cut(s) 426, 1082
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 960
NspI RCATGY 3 cut(s) 607, 712, 863
OliI CACNNNNGTG 1 cut(s) 248
PaeI GCATGC 1 cut(s) 712
PagI TCATGA 1 cut(s) 1006
PceI AGGCCT 1 cut(s) 536
PciI ACATGT 1 cut(s) 603
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 1 cut(s) 562
PkrI GCNGC 6 cut(s) 124, 418, 835, 916, 970, 1101
PleI GAGTC 2 cut(s) 466, 907
PpsI GAGTC 2 cut(s) 466, 907
PscI ACATGT 1 cut(s) 603
PsiI TTATAA 1 cut(s) 1070
Psp1406I AACGTT 1 cut(s) 1033
Psp6I CCWGG 1 cut(s) 664
PspFI CCCAGC 1 cut(s) 910
PspGI CCWGG 1 cut(s) 664
PspN4I GGNNCC 2 cut(s) 426, 1082
PspPI GGNCC 2 cut(s) 236, 929
PsrI GAACNNNNNNTAC 2 cut(s) 92, 124
PstI CTGCAG 1 cut(s) 127
PvuII CAGCTG 1 cut(s) 1099
RsaI GTAC 4 cut(s) 290, 426, 477, 743
RsaNI GTAC 4 cut(s) 289, 425, 476, 742
RseI CAYNNNNRTG 3 cut(s) 152, 248, 770
SaqAI TTAA 1 cut(s) 867
SatI GCNGC 6 cut(s) 123, 417, 834, 915, 969, 1100
Sau3AI GATC 4 cut(s) 144, 293, 313, 1018
Sau96I GGNCC 2 cut(s) 236, 929
SchI GAGTC 2 cut(s) 467, 908
ScrFI CCNGG 2 cut(s) 429, 666
SduI GDGCHC 1 cut(s) 580
SfaNI GCATC 6 cut(s) 586, 631, 736, 799, 894, 945
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 1 cut(s) 929
SmiMI CAYNNNNRTG 3 cut(s) 152, 248, 770
SmlI CTYRAG 2 cut(s) 1075, 1112
SmoI CTYRAG 2 cut(s) 1075, 1112
SphI GCATGC 1 cut(s) 712
Sse9I AATT 3 cut(s) 31, 332, 947
SseBI AGGCCT 1 cut(s) 536
SspMI CTAG 2 cut(s) 269, 356
StuI AGGCCT 1 cut(s) 536
StyD4I CCNGG 2 cut(s) 427, 664
StyI CCWWGG 1 cut(s) 618
TaaI ACNGT 2 cut(s) 71, 446
TaiI ACGT 2 cut(s) 1036, 1096
TaqI TCGA 3 cut(s) 44, 758, 888
TasI AATT 3 cut(s) 31, 332, 947
TatI WGTACW 1 cut(s) 475
TfiI GAWTC 1 cut(s) 562
Tru1I TTAA 1 cut(s) 867
Tru9I TTAA 1 cut(s) 867
TscAI CASTG 2 cut(s) 503, 846
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 6 cut(s) 122, 416, 833, 914, 968, 1099
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 5 cut(s) 35, 80, 191, 600, 810
TspRI CASTG 2 cut(s) 503, 846
VpaK11BI GGWCC 1 cut(s) 929
XapI RAATTY 1 cut(s) 332
XceI RCATGY 3 cut(s) 607, 712, 863
XmnI GAANNNNTTC 1 cut(s) 368
XspI CTAG 2 cut(s) 269, 356
Zsp2I ATGCAT 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.