Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0007353

Physical Location & Seq

Genomic Coordinates Forward (+)

31745564 .. 31750789

Gene Structure 5226 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g33520.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

ATGGCCATCGATTCTCTCGTTCTGGGCGCCGGGCAAGAGGTAGGCAAGAGCTGCGTGATCGTAACAATCAACGGAAAACGAATCATGTTCGACTGCGGTATGCACATGGGGCACCTCGACCACCGCCGCTACCCGGACTTCTCCCTCATCAACAACCAAACCCTCACTTGCATCGTCATCACTCACTTCCATCTGGATCACATCGGCGCCTTACCTTACTTCACCCAAGTCTGCGGTTACAATGGCCCCATTTACATGACGTATCCGACCAAGGCGCTGGCGCCGTTGATGTTAGAGGACTATAGGAAAATTATGGTTGAGAGAAGGGGTGAGGAGGAGCAGTTTACCTCGGAGCACATTGCCGAATGCATGAAGAAAGTTATAGCGGTGAATCTGAAGGAGACGGTGCAGGTTGATAAGGATCTTCAGATCCGTGCGTACTATGCAGGACATGTTCTTGGAGCTGCAATGTTTTATGCAAAGGTGGGAGATGCTGCTATGGTGTACACGGGAGACTATAATATGACACCAGATAGGCATCTTGGAGCAGCTCAAATTGATCGACTAAGTTTGGACCTTGTTATTACAGAGTCCACATATGCAACAACCATTCGTGATTCAAAATATCCCCGGGAAAGAGAATTTCTCAAAGCTGTTCATACTTGTGTTGCCGGTGGGGGAAAAGTGCTAATTCCTTCATTTGCTTTGGGAAGAGCTCAGGAGCTCTGTATCTTATTGGAAGACTACTGGGAGCGCATGAATTTAAAGGTTCCTATTTACTTCTCAACAGCTTTGACCCGTCAAGCCAATATGTATTATATGATGCTCATCAGTTGGACCAGCCAGAAAATCAAAGAAACACACTCCACACATAATGCTTTTGATTTCAAAAATGTTCACAAATTTGAACGGTCTATGATAGATGCTCCTGGACCTTGTGTTCTGTTTGCCGGACCAGGGATGATAAGTGGTGGTTTTTCACTTGAGGTTTTTAAGCACTGGGCTCCATCAGAAAAGAATCTTGTCATAATGCCTGGGTATTGCGTGGCTGGAACCATAGGACATAAATTAATGTCAGGTAAACCGACCAAAATTGATCTGGATAAAGACACCAGAATTGATGTGCGATGCCAGATTCATCAATTGGCTTTCAGCCCTCATACAGATGCCAAAGGGATTATGGATCTGGTGAAGTTTCTTTCTCCCAAGCACGTTATACTAGTTCATGGTGAGAAGCCTAAGATGGCTACTCTGAAAGGAAGAATCGAAACCGAACTGGGAATTCAATGCTATTATCCTGCAATTAATGAGACTGTGTCGATTCCTTCCACCCATTATGCGACAGCAGTGACTTCTGATGCATTTGTCCAAAGTTGCTCGAGTCCAAACTTCAAGTTTTCTACAAGCAGCTCAGAACACTCACAAGGTTCTAGTAACAGTAAATCCATTCCACGGCTGCGAGTAAGTGATGATAGGGTAGCCGAAGGGATCTTGGTTATGGAGAAAAACAAAAAGGCCAAGGTAGTGCACCAAGATGAGCTCCCGCTCATGTTAGGAGAAAAGAAGCACCAAGTCAAGTTTGCCTATTGCTGTCCTGCTCACATCGGTAACTCGCAAGAGAATACCAGTCTGCCTCTGTCATCAACAAACAATGATCTGCTTCAGCTATTTTCTGCAAAACTTGCAAATGAACTCTCCGAGGGGAACATTCAACATTTCGGAGATCACCTTCAGTTAGAGTCGATTCATGTCTCGATTTGTTCGAATAACGATTGTCCTTACAGATTAAGTGACAATGGTGCTGAAGAACAACGCGAGCCAGTGTTCTTCTGCTGTAGTTGGGAAATGGAAGATGAGAAGCTTGCATGGAAAGTCATCTCAATTTGTCAAAAACCGACGCTTCGAGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000741 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0009553 GO:0009559 GO:0009561 GO:0009987 GO:0010197 GO:0016043 GO:0030154 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Beta-Casp Lactamase_B_6 RMMBL zf-RING_2

Protein Analysis

637

Amino Acids

71.27

Weight (kDa)

6.83

Isoelectric Point (pI)

36.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	15 - 178	1.1e-11	Metallo-beta-lactamase superfamily
Anti-Pycsar_Apyc1	PF23023	17 - 179	1.1e-08	Anti-Pycsar protein Apyc1
Lactamase_B_6	PF16661	20 - 186	2.8e-20	Metallo-beta-lactamase superfamily domain
Lactamase_B_2	PF12706	27 - 193	6.6e-06	Beta-lactamase superfamily domain
Beta-Casp	PF10996	239 - 357	4.5e-29	Beta-Casp domain
RMMBL	PF07521	372 - 433	6.9e-17	Zn-dependent metallo-hydrolase RNA specificity domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007353)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01730 AT2G01730
fragaria_vesca	FvH4_4g33520
malus_domestica	MD16G1018300.v1.1
prunus_persica	Prupe.1G333600_v2.0.a1
pyrus_communis	pycom16g01670
rosa_chinensis	RchiOBHm_Chr3g0480131 RchiOBHm_Chr4g0443031
rosa_laevigata	RLG00000005961
rosa_multiflora	Rmu_co8263599.1_g000001 Rmu_sc0006604.1_g000001 Rmu_sc0006604.1_g000004 Rmu_sc0008055.1_g000002 Rmu_sc0011261.1_g000006
rosa_roxburghii	Rroxscaffold_5G00383660
rosa_rugosa	Rorug04G0343900
rosa_samantha	Rh3AG232100 Rh3CG261900 Rh3DG257700 Rh4AG398100 Rh4BG409600 Rh4CG425100 Rh4DG404400
rosa_wichuraiana	Rw3G020840 Rw4G034290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	400
AccB1I	GGYRCC	4 cut(s)	26, 111, 206, 280
AccBSI	CCGCTC	1 cut(s)	1546
AccII	CGCG	1 cut(s)	1815
AciI	CCGC	6 cut(s)	96, 124, 127, 234, 386, 1544
AclWI	GGATC	5 cut(s)	204, 424, 429, 1191, 1496
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	4 cut(s)	641, 760, 902, 1281
AcuI	CTGAAG	5 cut(s)	410, 416, 1646, 1715, 1824
AcyI	GRCGYC	3 cut(s)	27, 207, 281
AfaI	GTAC	2 cut(s)	440, 506
AfiI	CCNNNNNNNGG	3 cut(s)	133, 957, 1452
AflIII	ACRYGT	1 cut(s)	451
AgsI	TTSAA	6 cut(s)	621, 889, 908, 1286, 1393, 1712
AhlI	ACTAGT	1 cut(s)	1219
AjnI	CCWGG	3 cut(s)	928, 955, 1033
AloI	GAACNNNNNNTCC	2 cut(s)	924, 956
Alw21I	GWGCWC	5 cut(s)	357, 718, 726, 1530, 1542
Alw26I	GTCTC	4 cut(s)	395, 507, 1304, 1756
Alw44I	GTGCAC	1 cut(s)	1526
AlwI	GGATC	5 cut(s)	204, 424, 429, 1191, 1496
Ama87I	CYCGRG	2 cut(s)	630, 1378
AoxI	GGCC	3 cut(s)	3, 244, 1515
ApaLI	GTGCAC	1 cut(s)	1526
ApeKI	GCWGC	6 cut(s)	51, 464, 494, 548, 1407, 1456
ApoI	RAATTY	4 cut(s)	641, 760, 902, 1281
AseI	ATTAAT	2 cut(s)	1070, 1305
AspLEI	GCGC	5 cut(s)	29, 209, 277, 283, 756
AspS9I	GGNCC	5 cut(s)	245, 574, 837, 932, 953
AsuC2I	CCSGG	4 cut(s)	31, 134, 631, 632
AsuHPI	GGTGA	6 cut(s)	214, 341, 400, 1201, 1241, 1718
AsuII	TTCGAA	1 cut(s)	1763
AvaI	CYCGRG	2 cut(s)	630, 1378
AvaII	GGWCC	4 cut(s)	574, 837, 932, 953
BaeGI	GKGCMC	2 cut(s)	114, 1530
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	4 cut(s)	26, 111, 206, 280
BanII	GRGCYC	4 cut(s)	718, 726, 1006, 1542
BbsI	GAAGAC	1 cut(s)	747
Bbv12I	GWGCWC	5 cut(s)	357, 718, 726, 1530, 1542
BbvI	GCAGC	6 cut(s)	38, 451, 481, 560, 1419, 1443
BccI	CCATC	4 cut(s)	14, 198, 1015, 1237
BceAI	ACGGC	2 cut(s)	268, 1469
BciT130I	CCWGG	3 cut(s)	930, 957, 1035
BciVI	GTATCC	1 cut(s)	273
BcnI	CCSGG	4 cut(s)	31, 134, 631, 632
BcoDI	GTCTC	4 cut(s)	395, 507, 1304, 1756
BcuI	ACTAGT	1 cut(s)	1219
BfaI	CTAG	2 cut(s)	1220, 1431
BfmI	CTRYAG	2 cut(s)	301, 1834
BfoI	RGCGCY	4 cut(s)	30, 210, 278, 284
BfuAI	ACCTGC	1 cut(s)	400
BfuI	GTATCC	1 cut(s)	273
BisI	GCNGC	7 cut(s)	52, 127, 465, 495, 549, 1408, 1457
BlsI	GCNGC	7 cut(s)	53, 128, 466, 496, 550, 1409, 1458
Bme1390I	CCNGG	7 cut(s)	31, 134, 631, 632, 930, 957, 1035
Bme18I	GGWCC	4 cut(s)	574, 837, 932, 953
BmeT110I	CYCGRG	2 cut(s)	630, 1378
BmgT120I	GGNCC	5 cut(s)	245, 574, 837, 932, 953
BmiI	GGNNCC	8 cut(s)	28, 113, 208, 247, 282, 771, 1005, 1054
BmrFI	CCNGG	7 cut(s)	31, 134, 631, 632, 930, 957, 1035
BmrI	ACTGGG	3 cut(s)	757, 1009, 1286
BmsI	GCATC	8 cut(s)	180, 481, 547, 813, 913, 1118, 1156, 1348
BmuI	ACTGGG	3 cut(s)	757, 1009, 1286
BpiI	GAAGAC	1 cut(s)	747
BplI	GAGNNNNNCTC	2 cut(s)	630, 662
Bpu10I	CCTNAGC	1 cut(s)	717
Bpu14I	TTCGAA	1 cut(s)	1763
BpuEI	CTTGAG	1 cut(s)	1004
BpuMI	CCSGG	4 cut(s)	31, 134, 631, 632
Bsa29I	ATCGAT	1 cut(s)	9
BsaBI	GATNNNNATC	3 cut(s)	420, 537, 827
BsaHI	GRCGYC	3 cut(s)	27, 207, 281
BsaJI	CCNNGG	9 cut(s)	270, 348, 629, 630, 956, 1034, 1451, 1518, 1698
BsaXI	ACNNNNNCTCC	2 cut(s)	326, 356
Bsc4I	CCNNNNNNNGG	3 cut(s)	133, 957, 1452
Bse118I	RCCGGY	1 cut(s)	671
Bse1I	ACTGG	5 cut(s)	752, 1004, 1281, 1626, 1820
Bse3DI	GCAATG	2 cut(s)	357, 474
Bse8I	GATNNNNATC	3 cut(s)	420, 537, 827
BseBI	CCWGG	3 cut(s)	930, 957, 1035
BseCI	ATCGAT	1 cut(s)	9
BseDI	CCNNGG	9 cut(s)	270, 348, 629, 630, 956, 1034, 1451, 1518, 1698
BseGI	GGATG	1 cut(s)	966
BseJI	GATNNNNATC	3 cut(s)	420, 537, 827
BseLI	CCNNNNNNNGG	3 cut(s)	133, 957, 1452
BseMI	GCAATG	2 cut(s)	357, 474
BseMII	CTCAG	2 cut(s)	731, 1425
BseNI	ACTGG	5 cut(s)	752, 1004, 1281, 1626, 1820
BseRI	GAGGAG	2 cut(s)	347, 350
BseSI	GKGCMC	2 cut(s)	114, 1530
BseXI	GCAGC	6 cut(s)	38, 451, 481, 560, 1419, 1443
BsgI	GTGCAG	1 cut(s)	428
Bsh1236I	CGCG	1 cut(s)	1815
BshFI	GGCC	3 cut(s)	5, 246, 1517
BshNI	GGYRCC	4 cut(s)	26, 111, 206, 280
BshVI	ATCGAT	1 cut(s)	9
BsiHKAI	GWGCWC	5 cut(s)	357, 718, 726, 1530, 1542
BsiHKCI	CYCGRG	2 cut(s)	630, 1378
BsiSI	CCGG	5 cut(s)	30, 134, 631, 672, 951
BslI	CCNNNNNNNGG	3 cut(s)	133, 957, 1452
BsmAI	GTCTC	4 cut(s)	395, 507, 1304, 1756
BsmBI	CGTCTC	1 cut(s)	395
BsmI	GAATGC	1 cut(s)	371
BsnI	GGCC	3 cut(s)	5, 246, 1517
BsoBI	CYCGRG	2 cut(s)	630, 1378
Bsp119I	TTCGAA	1 cut(s)	1763
Bsp1286I	GDGCHC	7 cut(s)	114, 357, 718, 726, 1006, 1530, 1542
Bsp1407I	TGTACA	1 cut(s)	504
BspACI	CCGC	6 cut(s)	96, 124, 127, 234, 386, 1544
BspANI	GGCC	3 cut(s)	5, 246, 1517
BspCNI	CTCAG	2 cut(s)	730, 1424
BspDI	ATCGAT	1 cut(s)	9
BspFNI	CGCG	1 cut(s)	1815
BspLI	GGNNCC	8 cut(s)	28, 113, 208, 247, 282, 771, 1005, 1054
BspMI	ACCTGC	1 cut(s)	400
BspPI	GGATC	5 cut(s)	204, 424, 429, 1191, 1496
BspQI	GCTCTTC	1 cut(s)	706
BspT104I	TTCGAA	1 cut(s)	1763
BspT107I	GGYRCC	4 cut(s)	26, 111, 206, 280
BsrBI	CCGCTC	1 cut(s)	1546
BsrDI	GCAATG	2 cut(s)	357, 474
BsrFI	RCCGGY	1 cut(s)	671
BsrGI	TGTACA	1 cut(s)	504
BsrI	ACTGG	5 cut(s)	752, 1004, 1281, 1626, 1820
BssAI	RCCGGY	1 cut(s)	671
BssECI	CCNNGG	9 cut(s)	270, 348, 629, 630, 956, 1034, 1451, 1518, 1698
BssNI	GRCGYC	3 cut(s)	27, 207, 281
BssT1I	CCWWGG	2 cut(s)	270, 1518
Bst2UI	CCWGG	3 cut(s)	930, 957, 1035
Bst4CI	ACNGT	4 cut(s)	406, 912, 1315, 1439
Bst6I	CTCTTC	1 cut(s)	706
BstACI	GRCGYC	3 cut(s)	27, 207, 281
BstAPI	GCANNNNNTGC	2 cut(s)	51, 1682
BstAUI	TGTACA	1 cut(s)	504
BstBI	TTCGAA	1 cut(s)	1763
BstC8I	GCNNGC	3 cut(s)	279, 1817, 1863
BstDEI	CTNAG	4 cut(s)	566, 717, 1239, 1411
BstDSI	CCRYGG	1 cut(s)	1451
BstF5I	GGATG	1 cut(s)	966
BstFNI	CGCG	1 cut(s)	1815
BstH2I	RGCGCY	4 cut(s)	30, 210, 278, 284
BstHHI	GCGC	5 cut(s)	29, 209, 277, 283, 756
BstMAI	GTCTC	4 cut(s)	395, 507, 1304, 1756
BstMWI	GCNNNNNNNGC	4 cut(s)	51, 109, 443, 1682
BstNI	CCWGG	3 cut(s)	930, 957, 1035
BstNSI	RCATGY	1 cut(s)	455
BstSCI	CCNGG	7 cut(s)	29, 132, 629, 630, 928, 955, 1033
BstSFI	CTRYAG	2 cut(s)	301, 1834
BstSLI	GKGCMC	2 cut(s)	114, 1530
BstUI	CGCG	1 cut(s)	1815
BstV1I	GCAGC	6 cut(s)	38, 451, 481, 560, 1419, 1443
BstV2I	GAAGAC	1 cut(s)	747
BstX2I	RGATCY	4 cut(s)	421, 429, 1183, 1488
BstXI	CCANNNNNNTGG	1 cut(s)	277
BstYI	RGATCY	4 cut(s)	421, 429, 1183, 1488
Bsu15I	ATCGAT	1 cut(s)	9
BsuI	GTATCC	1 cut(s)	273
BsuRI	GGCC	3 cut(s)	5, 246, 1517
BsuTUI	ATCGAT	1 cut(s)	9
BtgI	CCRYGG	1 cut(s)	1451
BtgZI	GCGATG	1 cut(s)	1141
BtsCI	GGATG	1 cut(s)	966
BtsI	GCAGTG	1 cut(s)	1353
BtsIMutI	CAGTG	3 cut(s)	997, 1353, 1827
BveI	ACCTGC	1 cut(s)	400
Cac8I	GCNNGC	3 cut(s)	279, 1817, 1863
CfoI	GCGC	5 cut(s)	29, 209, 277, 283, 756
Cfr10I	RCCGGY	1 cut(s)	671
Cfr13I	GGNCC	5 cut(s)	245, 574, 837, 932, 953
Cfr9I	CCCGGG	1 cut(s)	630
ClaI	ATCGAT	1 cut(s)	9
CseI	GACGC	1 cut(s)	1906
Csp6I	GTAC	2 cut(s)	439, 505
CviQI	GTAC	2 cut(s)	439, 505
DdeI	CTNAG	4 cut(s)	566, 717, 1239, 1411
DinI	GGCGCC	3 cut(s)	28, 208, 282
DraI	TTTAAA	1 cut(s)	765
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	1 cut(s)	706
EarI	CTCTTC	1 cut(s)	706
Ecl136II	GAGCTC	3 cut(s)	716, 724, 1540
Eco130I	CCWWGG	2 cut(s)	270, 1518
Eco24I	GRGCYC	4 cut(s)	718, 726, 1006, 1542
Eco47I	GGWCC	4 cut(s)	574, 837, 932, 953
Eco53kI	GAGCTC	3 cut(s)	716, 724, 1540
Eco57I	CTGAAG	5 cut(s)	410, 416, 1646, 1715, 1824
Eco88I	CYCGRG	2 cut(s)	630, 1378
EcoICRI	GAGCTC	3 cut(s)	716, 724, 1540
EcoRI	GAATTC	1 cut(s)	1281
EcoRII	CCWGG	3 cut(s)	928, 955, 1033
EcoT14I	CCWWGG	2 cut(s)	270, 1518
EcoT22I	ATGCAT	2 cut(s)	371, 1363
EcoT38I	GRGCYC	4 cut(s)	718, 726, 1006, 1542
EgeI	GGCGCC	3 cut(s)	28, 208, 282
EheI	GGCGCC	3 cut(s)	28, 208, 282
ErhI	CCWWGG	2 cut(s)	270, 1518
Esp3I	CGTCTC	1 cut(s)	395
FauI	CCCGC	1 cut(s)	1551
FauNDI	CATATG	1 cut(s)	598
Fnu4HI	GCNGC	7 cut(s)	52, 127, 465, 495, 549, 1408, 1457
FokI	GGATG	1 cut(s)	973
FriOI	GRGCYC	4 cut(s)	718, 726, 1006, 1542
Fsp4HI	GCNGC	7 cut(s)	52, 127, 465, 495, 549, 1408, 1457
FspBI	CTAG	2 cut(s)	1220, 1431
GlaI	GCGC	5 cut(s)	28, 208, 276, 282, 755
GluI	GCNGC	7 cut(s)	52, 127, 465, 495, 549, 1408, 1457
HaeII	RGCGCY	4 cut(s)	30, 210, 278, 284
HaeIII	GGCC	3 cut(s)	5, 246, 1517
HapII	CCGG	5 cut(s)	30, 134, 631, 672, 951
HgaI	GACGC	1 cut(s)	1906
HhaI	GCGC	5 cut(s)	29, 209, 277, 283, 756
Hin1I	GRCGYC	3 cut(s)	27, 207, 281
Hin6I	GCGC	5 cut(s)	27, 207, 275, 281, 754
HinP1I	GCGC	5 cut(s)	27, 207, 275, 281, 754
HindIII	AAGCTT	1 cut(s)	1859
HpaII	CCGG	5 cut(s)	30, 134, 631, 672, 951
HphI	GGTGA	6 cut(s)	214, 341, 400, 1201, 1241, 1718
Hpy166II	GTNNAC	7 cut(s)	345, 505, 507, 594, 898, 1082, 1528
Hpy188III	TCNNGA	5 cut(s)	194, 614, 719, 1100, 1753
Hpy8I	GTNNAC	7 cut(s)	345, 505, 507, 594, 898, 1082, 1528
Hpy99I	CGWCG	1 cut(s)	1900
HpyAV	CCTTC	6 cut(s)	318, 391, 705, 1335, 1478, 1739
HpyCH4III	ACNGT	4 cut(s)	406, 912, 1315, 1439
HpyCH4IV	ACGT	2 cut(s)	260, 1212
HpyF10VI	GCNNNNNNNGC	4 cut(s)	51, 109, 443, 1682
HpyF3I	CTNAG	4 cut(s)	566, 717, 1239, 1411
HpySE526I	ACGT	2 cut(s)	260, 1212
Hsp92I	GRCGYC	3 cut(s)	27, 207, 281
HspAI	GCGC	5 cut(s)	27, 207, 275, 281, 754
KasI	GGCGCC	3 cut(s)	26, 206, 280
LguI	GCTCTTC	1 cut(s)	706
LmnI	GCTCC	9 cut(s)	337, 352, 461, 545, 721, 751, 931, 1009, 1545
Lsp1109I	GCAGC	6 cut(s)	38, 451, 481, 560, 1419, 1443
LweI	GCATC	8 cut(s)	180, 481, 547, 813, 913, 1118, 1156, 1348
MaeI	CTAG	2 cut(s)	1220, 1431
MaeII	ACGT	2 cut(s)	260, 1212
MaeIII	GTNAC	6 cut(s)	61, 236, 1348, 1433, 1607, 1790
MbiI	CCGCTC	1 cut(s)	1546
MboII	GAAGA	8 cut(s)	385, 416, 723, 752, 1272, 1817, 1819, 1862
MfeI	CAATTG	1 cut(s)	1142
MflI	RGATCY	4 cut(s)	421, 429, 1183, 1488
MhlI	GDGCHC	7 cut(s)	114, 357, 718, 726, 1006, 1530, 1542
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	3 cut(s)	27, 207, 281
MlyI	GAGTC	3 cut(s)	599, 1390, 1748
MmeI	TCCRAC	2 cut(s)	290, 815
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	2 cut(s)	371, 1363
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	5 cut(s)	764, 993, 1070, 1305, 1787
MslI	CAYNNNNRTG	4 cut(s)	254, 663, 1164, 1533
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	5 cut(s)	30, 134, 631, 672, 951
MspR9I	CCNGG	7 cut(s)	31, 134, 631, 632, 930, 957, 1035
MunI	CAATTG	1 cut(s)	1142
Mva1269I	GAATGC	1 cut(s)	371
MvaI	CCWGG	3 cut(s)	930, 957, 1035
MvnI	CGCG	1 cut(s)	1815
MwoI	GCNNNNNNNGC	4 cut(s)	51, 109, 443, 1682
NarI	GGCGCC	3 cut(s)	27, 207, 281
NciI	CCSGG	4 cut(s)	31, 134, 631, 632
NdeI	CATATG	1 cut(s)	598
NlaIV	GGNNCC	8 cut(s)	28, 113, 208, 247, 282, 771, 1005, 1054
NmuCI	GTSAC	2 cut(s)	1348, 1790
NsiI	ATGCAT	2 cut(s)	371, 1363
NspI	RCATGY	1 cut(s)	455
NspV	TTCGAA	1 cut(s)	1763
PaeR7I	CTCGAG	1 cut(s)	1378
PciI	ACATGT	1 cut(s)	451
PciSI	GCTCTTC	1 cut(s)	706
PcsI	WCGNNNNNNNCGW	2 cut(s)	15, 1760
PctI	GAATGC	1 cut(s)	371
PfeI	GAWTC	9 cut(s)	11, 81, 391, 617, 1018, 1135, 1263, 1321, 1744
PflFI	GACNNNGTC	1 cut(s)	1315
PfoI	TCCNGGA	1 cut(s)	928
PkrI	GCNGC	7 cut(s)	53, 128, 466, 496, 550, 1409, 1458
PleI	GAGTC	3 cut(s)	598, 1389, 1747
PluTI	GGCGCC	3 cut(s)	30, 210, 284
PpsI	GAGTC	3 cut(s)	598, 1389, 1747
PscI	ACATGT	1 cut(s)	451
PshBI	ATTAAT	2 cut(s)	1070, 1305
Psp124BI	GAGCTC	3 cut(s)	718, 726, 1542
Psp6I	CCWGG	3 cut(s)	928, 955, 1033
PspGI	CCWGG	3 cut(s)	928, 955, 1033
PspN4I	GGNNCC	8 cut(s)	28, 113, 208, 247, 282, 771, 1005, 1054
PspPI	GGNCC	5 cut(s)	245, 574, 837, 932, 953
PspXI	VCTCGAGB	1 cut(s)	1378
PsuI	RGATCY	4 cut(s)	421, 429, 1183, 1488
PsyI	GACNNNGTC	1 cut(s)	1315
RsaI	GTAC	2 cut(s)	440, 506
RsaNI	GTAC	2 cut(s)	439, 505
RseI	CAYNNNNRTG	4 cut(s)	254, 663, 1164, 1533
SacI	GAGCTC	3 cut(s)	718, 726, 1542
SapI	GCTCTTC	1 cut(s)	706
SaqAI	TTAA	5 cut(s)	764, 993, 1070, 1305, 1787
SatI	GCNGC	7 cut(s)	52, 127, 465, 495, 549, 1408, 1457
Sau96I	GGNCC	5 cut(s)	245, 574, 837, 932, 953
SchI	GAGTC	3 cut(s)	599, 1390, 1748
ScrFI	CCNGG	7 cut(s)	31, 134, 631, 632, 930, 957, 1035
SduI	GDGCHC	7 cut(s)	114, 357, 718, 726, 1006, 1530, 1542
SfaNI	GCATC	8 cut(s)	180, 481, 547, 813, 913, 1118, 1156, 1348
SfcI	CTRYAG	2 cut(s)	301, 1834
SfoI	GGCGCC	3 cut(s)	28, 208, 282
Sfr274I	CTCGAG	1 cut(s)	1378
SfuI	TTCGAA	1 cut(s)	1763
SinI	GGWCC	4 cut(s)	574, 837, 932, 953
SlaI	CTCGAG	1 cut(s)	1378
SmaI	CCCGGG	1 cut(s)	632
SmiMI	CAYNNNNRTG	4 cut(s)	254, 663, 1164, 1533
SmlI	CTYRAG	2 cut(s)	983, 1378
SmoI	CTYRAG	2 cut(s)	983, 1378
SpeI	ACTAGT	1 cut(s)	1219
SsiI	CCGC	6 cut(s)	96, 124, 127, 234, 386, 1544
SspDI	GGCGCC	3 cut(s)	26, 206, 280
SspMI	CTAG	2 cut(s)	1220, 1431
SstI	GAGCTC	3 cut(s)	718, 726, 1542
StyD4I	CCNGG	7 cut(s)	29, 132, 629, 630, 928, 955, 1033
StyI	CCWWGG	2 cut(s)	270, 1518
TaaI	ACNGT	4 cut(s)	406, 912, 1315, 1439
TaiI	ACGT	2 cut(s)	263, 1215
TatI	WGTACW	1 cut(s)	504
TauI	GCSGC	1 cut(s)	129
TfiI	GAWTC	9 cut(s)	11, 81, 391, 617, 1018, 1135, 1263, 1321, 1744
Tru1I	TTAA	5 cut(s)	764, 993, 1070, 1305, 1787
Tru9I	TTAA	5 cut(s)	764, 993, 1070, 1305, 1787
TscAI	CASTG	3 cut(s)	1004, 1353, 1827
TseFI	GTSAC	2 cut(s)	1348, 1790
TseI	GCWGC	6 cut(s)	51, 464, 494, 548, 1407, 1456
Tsp45I	GTSAC	2 cut(s)	1348, 1790
TspDTI	ATGAA	8 cut(s)	386, 647, 687, 773, 1127, 1214, 1704, 1736
TspGWI	ACGGA	2 cut(s)	87, 422
TspMI	CCCGGG	1 cut(s)	630
TspRI	CASTG	3 cut(s)	1004, 1353, 1827
Tth111I	GACNNNGTC	1 cut(s)	1315
VneI	GTGCAC	1 cut(s)	1526
VpaK11BI	GGWCC	4 cut(s)	574, 837, 932, 953
VspI	ATTAAT	2 cut(s)	1070, 1305
XapI	RAATTY	4 cut(s)	641, 760, 902, 1281
XceI	RCATGY	1 cut(s)	455
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1096, 1177
XhoI	CTCGAG	1 cut(s)	1378
XmaI	CCCGGG	1 cut(s)	630
XspI	CTAG	2 cut(s)	1220, 1431
Zsp2I	ATGCAT	2 cut(s)	371, 1363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.