Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0007353

Physical Location & Seq

Genomic Coordinates Forward (+)

61423271 .. 61427966

Gene Structure 4696 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G034290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1947 bp

ATGGCCATCGACTCTCTCGTTCTGGGCGCCGGGCAAGAGGTAGGCAAGAGCTGTGTGATTGTGACGATCAACGGGAAACGAATCATGTTCGACTGTGGTATGCATATGGGCCACCTCGACCACCGCCGCTACCCTGACTTCTCCCTCATCGGCCCAAATTTCAATCAAACCCTCACTTGCATCATCATCACTCACTTTCATCTGGATCACATCGGCGCGCTCCCTTACTTCACTGAAGTGTGCGGTTACAGTGGCCCCATTTACATGACGTATCCGACGAAGGCGCTGGCGCCTTTGATGTTGGAGGACTATCGGAAAGTGATGGTTGAGAGAAGGGGCGAGGAGGAACAGTTTACGTCCGAGCTTATTGTTGAATGCATGAAGAAAGTTATACCGGTGGATCTCAAGCAGACGTTGCAGGTTGATAAGGATCTTCAAATCCGTGCTTACTATGCCGGACATGTTCTTGGAGCTGCAATGTTTTATGCAAAGGTGGGAGACGCTGCTATGGTGTACACAGGAGACTATAATATGACACCAGACAGGCATCTTGGAGCAGCTCAAATTGATCGACTAAGTTTGGACCTTGTTATTACAGAGTATGGGATGTCCACATATGCAACAACCATTCGTGATTCAAAATATGCCCGGGAAAGAGAATTTCTCAGAGCTGTTCACACTTGTGTTGCTGGTGGGGGAAAAGTGCTAATTCCTTCATTTGCTCTGGGAAGAGCTCAGGAACTCTGTATCTTATTGGAAGACTACTGGGAGCGCATGAATTTAAAGGTTCCTATTTACTTCTCAGCAGGTTTGACCCGTCAAGCCAATATGTATTATAAGATGCTCATCAGTTGGACCAGCCAGAAAATCAAAGAAACATACTCCACACATAATGCATTTGATTTTAAAAATGTTCACAAATTTGAGCGGTCTATGATAGATGCTCCTGGACCTTGTGTTCTGTTTGCCACACCAGGGATGATCAGTGGTGGTTTTTCACTTGAGGTTTTTAAGCACTGGGCTCCTTCAGAAAAGAATCTTGTTACAATGCCAGGGTATTGCGTGGCTGGAACCATAGGACATAAATTAATGTCTGGTAAACCGACCAAAATTGATCTGGACAAGGACACCCGAATTGATGTGCGATGCCAGATTCATCAATTGACTTTCAGTCCTCATACAGATGCCAAAGGGATTATGGATCTGGTGAAGTTTCTTTCTCCCAAGCACGTGATACTTGTTCATGGTGAGAAGCCTAGGATGGCTACTCTGAAAGGAAGAATTCAATCCGAACTGGGAATTCAATGCTACGACCCTGCAAACAATGAGACCGTGTCAGTTCCGTCCACCCACTATGCGACAGCAGTAGCTTCCGATGCATTTGTCCAAAGTTGCTCAAATCCAAACTTCAAGTTTTCTACAGGCGGCTCAGAACACAAACAAGGTTCGAGTTCGAGCAACAGAAAATCCATTCCTCGACTGCAAGTAAGTGATGATAGGGTAGGCGAAGGGATCTTGGTTATGGAGAAAAACAAAAAAGCCAAGGTAGTGCACCAAGATGAGCTCCTGGTCATGTTAGAAGAAAAGAAGCACCAAGTCAAGTTTGCATATTGCTGCCCTGCTCACATTGGTAACTTGCAAGAGAATCAAGAGACCGAAGGCACCGGCCTGCCTCTGTCTTCAACAAACAATGATCTGCTTCAGCTATTGTCTGCAAAACTCGCAAATGAACTCTCCGAGGGGAGCATTCAAGATTTCGGGGATCACCTTCAAGTGGAGTCGTTTCGCGTGTCCATTTGTTCAAAGGAGGATTGCCCTTACAGGTTAAGTGACAACGCTGGTGAAGGACACTATGAATTACCAGTGTTCTTTTGCTGCAGTTGGGCAATGGAAGATGAGAAGCTTGCATGGAAAGTCATCTCAGTGTGTCAAAAGTCAACGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000741 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0009553 GO:0009559 GO:0009561 GO:0009987 GO:0010197 GO:0016043 GO:0030154 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Beta-Casp Lactamase_B_6 RMMBL zf-RING_2

Protein Analysis

648

Amino Acids

72.48

Weight (kDa)

6.56

Isoelectric Point (pI)

37.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	15 - 181	1.2e-09	Metallo-beta-lactamase superfamily
Anti-Pycsar_Apyc1	PF23023	17 - 182	1.8e-08	Anti-Pycsar protein Apyc1
Lactamase_B_6	PF16661	20 - 189	1.9e-19	Metallo-beta-lactamase superfamily domain
Beta-Casp	PF10996	245 - 363	2.9e-28	Beta-Casp domain
RMMBL	PF07521	378 - 437	9.8e-16	Zn-dependent metallo-hydrolase RNA specificity domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007353)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01730 AT2G01730
fragaria_vesca	FvH4_4g33520
malus_domestica	MD16G1018300.v1.1
prunus_persica	Prupe.1G333600_v2.0.a1
pyrus_communis	pycom16g01670
rosa_chinensis	RchiOBHm_Chr3g0480131 RchiOBHm_Chr4g0443031
rosa_laevigata	RLG00000005961
rosa_multiflora	Rmu_co8263599.1_g000001 Rmu_sc0006604.1_g000001 Rmu_sc0006604.1_g000004 Rmu_sc0008055.1_g000002 Rmu_sc0011261.1_g000006
rosa_roxburghii	Rroxscaffold_5G00383660
rosa_rugosa	Rorug04G0343900
rosa_samantha	Rh3AG232100 Rh3CG261900 Rh3DG257700 Rh4AG398100 Rh4BG409600 Rh4CG425100 Rh4DG404400
rosa_wichuraiana	Rw3G020840 Rw4G034290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	837
Acc36I	ACCTGC	2 cut(s)	409, 797
AccB1I	GGYRCC	3 cut(s)	26, 289, 1661
AccBSI	CCGCTC	1 cut(s)	928
AccII	CGCG	2 cut(s)	218, 1788
AciI	CCGC	5 cut(s)	124, 127, 243, 928, 1425
AclWI	GGATC	6 cut(s)	213, 408, 438, 1209, 1520, 1770
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	6 cut(s)	157, 659, 778, 920, 1281, 1299
AcuI	CTGAAG	3 cut(s)	255, 1011, 1685
AcvI	CACGTG	1 cut(s)	1231
AcyI	GRCGYC	2 cut(s)	27, 290
AfaI	GTAC	1 cut(s)	515
AfiI	CCNNNNNNNGG	2 cut(s)	975, 1774
AflIII	ACRYGT	1 cut(s)	460
AgeI	ACCGGT	1 cut(s)	394
AhdI	GACNNNNNGTC	1 cut(s)	1170
AjnI	CCWGG	4 cut(s)	946, 973, 1051, 1566
AleI	CACNNNNGTG	2 cut(s)	236, 681
AloI	GAACNNNNNNTCC	2 cut(s)	942, 974
Alw21I	GWGCWC	3 cut(s)	736, 1554, 1566
Alw26I	GTCTC	4 cut(s)	492, 516, 1322, 1646
Alw44I	GTGCAC	1 cut(s)	1550
AlwI	GGATC	6 cut(s)	213, 408, 438, 1209, 1520, 1770
Ama87I	CYCGRG	1 cut(s)	648
AoxI	GGCC	5 cut(s)	3, 109, 151, 253, 1666
ApaLI	GTGCAC	1 cut(s)	1550
ApeKI	GCWGC	5 cut(s)	473, 503, 557, 1614, 1875
ApoI	RAATTY	6 cut(s)	157, 659, 778, 920, 1281, 1299
AseI	ATTAAT	1 cut(s)	1088
AsiGI	ACCGGT	1 cut(s)	394
AspA2I	CCTAGG	1 cut(s)	1256
AspLEI	GCGC	6 cut(s)	29, 218, 220, 286, 292, 774
AspS9I	GGNCC	6 cut(s)	109, 152, 254, 583, 855, 950
AsuC2I	CCSGG	3 cut(s)	31, 649, 650
AsuHPI	GGTGA	4 cut(s)	1219, 1259, 1757, 1853
AvaI	CYCGRG	1 cut(s)	648
AvaII	GGWCC	3 cut(s)	583, 855, 950
AvrII	CCTAGG	1 cut(s)	1256
BaeGI	GKGCMC	1 cut(s)	1554
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	3 cut(s)	26, 289, 1661
BanII	GRGCYC	3 cut(s)	736, 1024, 1566
BbrPI	CACGTG	1 cut(s)	1231
BbsI	GAAGAC	2 cut(s)	765, 1671
Bbv12I	GWGCWC	3 cut(s)	736, 1554, 1566
BbvI	GCAGC	5 cut(s)	460, 490, 569, 1601, 1862
BccI	CCATC	3 cut(s)	14, 316, 1255
BciT130I	CCWGG	4 cut(s)	948, 975, 1053, 1568
BciVI	GTATCC	1 cut(s)	282
BclI	TGATCA	1 cut(s)	981
BcnI	CCSGG	3 cut(s)	31, 649, 650
BcoDI	GTCTC	4 cut(s)	492, 516, 1322, 1646
BfaI	CTAG	1 cut(s)	1257
BfmI	CTRYAG	2 cut(s)	1419, 1876
BfoI	RGCGCY	3 cut(s)	30, 287, 293
BfuAI	ACCTGC	2 cut(s)	409, 797
BfuI	GTATCC	1 cut(s)	282
BisI	GCNGC	7 cut(s)	127, 474, 504, 558, 1426, 1615, 1876
BlnI	CCTAGG	1 cut(s)	1256
BlsI	GCNGC	7 cut(s)	128, 475, 505, 559, 1427, 1616, 1877
Bme1390I	CCNGG	7 cut(s)	31, 649, 650, 948, 975, 1053, 1568
Bme18I	GGWCC	3 cut(s)	583, 855, 950
BmeRI	GACNNNNNGTC	1 cut(s)	1170
BmeT110I	CYCGRG	1 cut(s)	648
BmgT120I	GGNCC	6 cut(s)	109, 152, 254, 583, 855, 950
BmiI	GGNNCC	7 cut(s)	28, 256, 291, 789, 1023, 1072, 1663
BmrFI	CCNGG	7 cut(s)	31, 649, 650, 948, 975, 1053, 1568
BmrI	ACTGGG	3 cut(s)	775, 1027, 1304
BmsI	GCATC	7 cut(s)	189, 556, 831, 931, 1136, 1174, 1366
BmuI	ACTGGG	3 cut(s)	775, 1027, 1304
BpiI	GAAGAC	2 cut(s)	765, 1671
BplI	GAGNNNNNCTC	2 cut(s)	648, 680
Bpu10I	CCTNAGC	1 cut(s)	735
BpuEI	CTTGAG	2 cut(s)	389, 1022
BpuMI	CCSGG	3 cut(s)	31, 649, 650
BsaAI	YACGTR	1 cut(s)	1231
BsaBI	GATNNNNATC	2 cut(s)	429, 845
BsaHI	GRCGYC	2 cut(s)	27, 290
BsaI	GGTCTC	2 cut(s)	1322, 1646
BsaJI	CCNNGG	6 cut(s)	648, 974, 1052, 1256, 1542, 1737
BsaWI	WCCGGW	1 cut(s)	394
BsaXI	ACNNNNNCTCC	2 cut(s)	335, 365
Bsc4I	CCNNNNNNNGG	2 cut(s)	975, 1774
Bse118I	RCCGGY	2 cut(s)	394, 1664
Bse1I	ACTGG	4 cut(s)	770, 1022, 1299, 1862
Bse3DI	GCAATG	2 cut(s)	483, 1893
Bse8I	GATNNNNATC	2 cut(s)	429, 845
BseBI	CCWGG	4 cut(s)	948, 975, 1053, 1568
BseDI	CCNNGG	6 cut(s)	648, 974, 1052, 1256, 1542, 1737
BseGI	GGATG	3 cut(s)	612, 984, 1266
BseJI	GATNNNNATC	2 cut(s)	429, 845
BseLI	CCNNNNNNNGG	2 cut(s)	975, 1774
BseMI	GCAATG	2 cut(s)	483, 1893
BseMII	CTCAG	5 cut(s)	679, 749, 816, 1443, 1935
BseNI	ACTGG	4 cut(s)	770, 1022, 1299, 1862
BsePI	GCGCGC	1 cut(s)	216
BseRI	GAGGAG	1 cut(s)	356
BseSI	GKGCMC	1 cut(s)	1554
BseXI	GCAGC	5 cut(s)	460, 490, 569, 1601, 1862
Bsh1236I	CGCG	2 cut(s)	218, 1788
BshFI	GGCC	5 cut(s)	5, 111, 153, 255, 1668
BshNI	GGYRCC	3 cut(s)	26, 289, 1661
BshTI	ACCGGT	1 cut(s)	394
BsiHKAI	GWGCWC	3 cut(s)	736, 1554, 1566
BsiHKCI	CYCGRG	1 cut(s)	648
BsiSI	CCGG	5 cut(s)	30, 395, 456, 649, 1665
BslI	CCNNNNNNNGG	2 cut(s)	975, 1774
BsmAI	GTCTC	4 cut(s)	492, 516, 1322, 1646
BsmBI	CGTCTC	1 cut(s)	492
BsmI	GAATGC	2 cut(s)	380, 1746
BsnI	GGCC	5 cut(s)	5, 111, 153, 255, 1668
Bso31I	GGTCTC	2 cut(s)	1322, 1646
BsoBI	CYCGRG	1 cut(s)	648
Bsp1286I	GDGCHC	4 cut(s)	736, 1024, 1554, 1566
Bsp1407I	TGTACA	1 cut(s)	513
BspACI	CCGC	5 cut(s)	124, 127, 243, 928, 1425
BspANI	GGCC	5 cut(s)	5, 111, 153, 255, 1668
BspCNI	CTCAG	5 cut(s)	678, 748, 815, 1442, 1934
BspFNI	CGCG	2 cut(s)	218, 1788
BspLI	GGNNCC	7 cut(s)	28, 256, 291, 789, 1023, 1072, 1663
BspMAI	CTGCAG	1 cut(s)	1880
BspMI	ACCTGC	2 cut(s)	409, 797
BspPI	GGATC	6 cut(s)	213, 408, 438, 1209, 1520, 1770
BspQI	GCTCTTC	1 cut(s)	724
BspT107I	GGYRCC	3 cut(s)	26, 289, 1661
BspTNI	GGTCTC	2 cut(s)	1322, 1646
BsrBI	CCGCTC	1 cut(s)	928
BsrDI	GCAATG	2 cut(s)	483, 1893
BsrFI	RCCGGY	2 cut(s)	394, 1664
BsrGI	TGTACA	1 cut(s)	513
BsrI	ACTGG	4 cut(s)	770, 1022, 1299, 1862
BssAI	RCCGGY	2 cut(s)	394, 1664
BssECI	CCNNGG	6 cut(s)	648, 974, 1052, 1256, 1542, 1737
BssHII	GCGCGC	1 cut(s)	216
BssNI	GRCGYC	2 cut(s)	27, 290
BssT1I	CCWWGG	2 cut(s)	1256, 1542
Bst2UI	CCWGG	4 cut(s)	948, 975, 1053, 1568
Bst4CI	ACNGT	4 cut(s)	95, 251, 351, 1333
Bst6I	CTCTTC	1 cut(s)	724
BstACI	GRCGYC	2 cut(s)	27, 290
BstAPI	GCANNNNNTGC	1 cut(s)	415
BstAUI	TGTACA	1 cut(s)	513
BstBAI	YACGTR	1 cut(s)	1231
BstC8I	GCNNGC	4 cut(s)	218, 288, 1670, 1905
BstDEI	CTNAG	6 cut(s)	575, 665, 735, 802, 1429, 1921
BstF5I	GGATG	3 cut(s)	612, 984, 1266
BstFNI	CGCG	2 cut(s)	218, 1788
BstH2I	RGCGCY	3 cut(s)	30, 287, 293
BstHHI	GCGC	6 cut(s)	29, 218, 220, 286, 292, 774
BstMAI	GTCTC	4 cut(s)	492, 516, 1322, 1646
BstMWI	GCNNNNNNNGC	4 cut(s)	415, 452, 1376, 1721
BstNI	CCWGG	4 cut(s)	948, 975, 1053, 1568
BstNSI	RCATGY	1 cut(s)	464
BstSCI	CCNGG	7 cut(s)	29, 647, 648, 946, 973, 1051, 1566
BstSFI	CTRYAG	2 cut(s)	1419, 1876
BstSLI	GKGCMC	1 cut(s)	1554
BstUI	CGCG	2 cut(s)	218, 1788
BstV1I	GCAGC	5 cut(s)	460, 490, 569, 1601, 1862
BstV2I	GAAGAC	2 cut(s)	765, 1671
BstX2I	RGATCY	4 cut(s)	400, 430, 1201, 1512
BstYI	RGATCY	4 cut(s)	400, 430, 1201, 1512
BsuI	GTATCC	1 cut(s)	282
BsuRI	GGCC	5 cut(s)	5, 111, 153, 255, 1668
BtgZI	GCGATG	1 cut(s)	1159
BtsCI	GGATG	3 cut(s)	612, 984, 1266
BtsIMutI	CAGTG	6 cut(s)	231, 256, 991, 1015, 1869, 1929
BveI	ACCTGC	2 cut(s)	409, 797
Cac8I	GCNNGC	4 cut(s)	218, 288, 1670, 1905
CfoI	GCGC	6 cut(s)	29, 218, 220, 286, 292, 774
Cfr10I	RCCGGY	2 cut(s)	394, 1664
Cfr13I	GGNCC	6 cut(s)	109, 152, 254, 583, 855, 950
Cfr9I	CCCGGG	1 cut(s)	648
CseI	GACGC	1 cut(s)	509
Csp6I	GTAC	1 cut(s)	514
CspAI	ACCGGT	1 cut(s)	394
CviAII	CATG	8 cut(s)	85, 265, 379, 461, 775, 1244, 1573, 1908
CviQI	GTAC	1 cut(s)	514
DdeI	CTNAG	6 cut(s)	575, 665, 735, 802, 1429, 1921
DinI	GGCGCC	2 cut(s)	28, 291
DraI	TTTAAA	2 cut(s)	783, 907
DriI	GACNNNNNGTC	1 cut(s)	1170
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	1 cut(s)	724
Eam1105I	GACNNNNNGTC	1 cut(s)	1170
EarI	CTCTTC	1 cut(s)	724
Ecl136II	GAGCTC	2 cut(s)	734, 1564
Eco130I	CCWWGG	2 cut(s)	1256, 1542
Eco24I	GRGCYC	3 cut(s)	736, 1024, 1566
Eco31I	GGTCTC	2 cut(s)	1322, 1646
Eco47I	GGWCC	3 cut(s)	583, 855, 950
Eco53kI	GAGCTC	2 cut(s)	734, 1564
Eco57I	CTGAAG	3 cut(s)	255, 1011, 1685
Eco72I	CACGTG	1 cut(s)	1231
Eco88I	CYCGRG	1 cut(s)	648
EcoICRI	GAGCTC	2 cut(s)	734, 1564
EcoRI	GAATTC	2 cut(s)	1281, 1299
EcoRII	CCWGG	4 cut(s)	946, 973, 1051, 1566
EcoT14I	CCWWGG	2 cut(s)	1256, 1542
EcoT22I	ATGCAT	4 cut(s)	105, 380, 898, 1381
EcoT38I	GRGCYC	3 cut(s)	736, 1024, 1566
EgeI	GGCGCC	2 cut(s)	28, 291
EheI	GGCGCC	2 cut(s)	28, 291
ErhI	CCWWGG	2 cut(s)	1256, 1542
Esp3I	CGTCTC	1 cut(s)	492
FaeI	CATG	8 cut(s)	88, 268, 382, 464, 778, 1247, 1576, 1911
FatI	CATG	8 cut(s)	84, 264, 378, 460, 774, 1243, 1572, 1907
FauNDI	CATATG	2 cut(s)	105, 616
FbaI	TGATCA	1 cut(s)	981
Fnu4HI	GCNGC	7 cut(s)	127, 474, 504, 558, 1426, 1615, 1876
FokI	GGATG	3 cut(s)	619, 991, 1273
FriOI	GRGCYC	3 cut(s)	736, 1024, 1566
Fsp4HI	GCNGC	7 cut(s)	127, 474, 504, 558, 1426, 1615, 1876
FspBI	CTAG	1 cut(s)	1257
GlaI	GCGC	6 cut(s)	28, 217, 219, 285, 291, 773
GluI	GCNGC	7 cut(s)	127, 474, 504, 558, 1426, 1615, 1876
HaeII	RGCGCY	3 cut(s)	30, 287, 293
HaeIII	GGCC	5 cut(s)	5, 111, 153, 255, 1668
HapII	CCGG	5 cut(s)	30, 395, 456, 649, 1665
HgaI	GACGC	1 cut(s)	509
HhaI	GCGC	6 cut(s)	29, 218, 220, 286, 292, 774
Hin1I	GRCGYC	2 cut(s)	27, 290
Hin1II	CATG	8 cut(s)	88, 268, 382, 464, 778, 1247, 1576, 1911
Hin6I	GCGC	6 cut(s)	27, 216, 218, 284, 290, 772
HinP1I	GCGC	6 cut(s)	27, 216, 218, 284, 290, 772
HincII	GTYRAC	1 cut(s)	1938
HindII	GTYRAC	1 cut(s)	1938
HindIII	AAGCTT	1 cut(s)	1901
HinfI	GANTC	7 cut(s)	11, 81, 635, 1036, 1153, 1645, 1778
HpaII	CCGG	5 cut(s)	30, 395, 456, 649, 1665
HphI	GGTGA	4 cut(s)	1219, 1259, 1757, 1853
Hpy188III	TCNNGA	6 cut(s)	203, 632, 737, 1118, 1649, 1751
Hpy99I	CGWCG	1 cut(s)	280
HpyAV	CCTTC	8 cut(s)	274, 327, 723, 1035, 1502, 1652, 1778, 1838
HpyCH4III	ACNGT	4 cut(s)	95, 251, 351, 1333
HpyCH4IV	ACGT	4 cut(s)	269, 356, 413, 1230
HpyF10VI	GCNNNNNNNGC	4 cut(s)	415, 452, 1376, 1721
HpyF3I	CTNAG	6 cut(s)	575, 665, 735, 802, 1429, 1921
HpySE526I	ACGT	4 cut(s)	269, 356, 413, 1230
Hsp92I	GRCGYC	2 cut(s)	27, 290
Hsp92II	CATG	8 cut(s)	88, 268, 382, 464, 778, 1247, 1576, 1911
HspAI	GCGC	6 cut(s)	27, 216, 218, 284, 290, 772
KasI	GGCGCC	2 cut(s)	26, 289
Ksp22I	TGATCA	1 cut(s)	981
LguI	GCTCTTC	1 cut(s)	724
LmnI	GCTCC	8 cut(s)	225, 470, 554, 769, 949, 1027, 1569, 1743
Lsp1109I	GCAGC	5 cut(s)	460, 490, 569, 1601, 1862
LweI	GCATC	7 cut(s)	189, 556, 831, 931, 1136, 1174, 1366
MaeI	CTAG	1 cut(s)	1257
MaeII	ACGT	4 cut(s)	269, 356, 413, 1230
MaeIII	GTNAC	5 cut(s)	61, 245, 1042, 1631, 1829
MbiI	CCGCTC	1 cut(s)	928
MboII	GAAGA	8 cut(s)	394, 425, 741, 770, 1290, 1592, 1671, 1904
MfeI	CAATTG	1 cut(s)	1160
MflI	RGATCY	4 cut(s)	400, 430, 1201, 1512
MhlI	GDGCHC	4 cut(s)	736, 1024, 1554, 1566
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	2 cut(s)	27, 290
MlyI	GAGTC	2 cut(s)	5, 1787
MmeI	TCCRAC	3 cut(s)	282, 299, 833
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	4 cut(s)	105, 380, 898, 1381
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	5 cut(s)	782, 906, 1011, 1088, 1826
MslI	CAYNNNNRTG	6 cut(s)	236, 263, 681, 1182, 1557, 1922
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	5 cut(s)	30, 395, 456, 649, 1665
MspR9I	CCNGG	7 cut(s)	31, 649, 650, 948, 975, 1053, 1568
MunI	CAATTG	1 cut(s)	1160
Mva1269I	GAATGC	2 cut(s)	380, 1746
MvaI	CCWGG	4 cut(s)	948, 975, 1053, 1568
MvnI	CGCG	2 cut(s)	218, 1788
MwoI	GCNNNNNNNGC	4 cut(s)	415, 452, 1376, 1721
NarI	GGCGCC	2 cut(s)	27, 290
NciI	CCSGG	3 cut(s)	31, 649, 650
NdeI	CATATG	2 cut(s)	105, 616
NlaIII	CATG	8 cut(s)	88, 268, 382, 464, 778, 1247, 1576, 1911
NlaIV	GGNNCC	7 cut(s)	28, 256, 291, 789, 1023, 1072, 1663
NmuCI	GTSAC	2 cut(s)	61, 1829
NsiI	ATGCAT	4 cut(s)	105, 380, 898, 1381
NspI	RCATGY	1 cut(s)	464
OliI	CACNNNNGTG	2 cut(s)	236, 681
PauI	GCGCGC	1 cut(s)	216
PciI	ACATGT	1 cut(s)	460
PciSI	GCTCTTC	1 cut(s)	724
PcsI	WCGNNNNNNNCGW	2 cut(s)	15, 275
PctI	GAATGC	2 cut(s)	380, 1746
PfeI	GAWTC	5 cut(s)	81, 635, 1036, 1153, 1645
PflFI	GACNNNGTC	1 cut(s)	1333
PfoI	TCCNGGA	1 cut(s)	946
PinAI	ACCGGT	1 cut(s)	394
PkrI	GCNGC	7 cut(s)	128, 475, 505, 559, 1427, 1616, 1877
PleI	GAGTC	2 cut(s)	5, 1786
PluTI	GGCGCC	2 cut(s)	30, 293
PmaCI	CACGTG	1 cut(s)	1231
PmlI	CACGTG	1 cut(s)	1231
PpsI	GAGTC	2 cut(s)	5, 1786
Ppu21I	YACGTR	1 cut(s)	1231
PscI	ACATGT	1 cut(s)	460
PshBI	ATTAAT	1 cut(s)	1088
PsiI	TTATAA	1 cut(s)	837
Psp124BI	GAGCTC	2 cut(s)	736, 1566
Psp6I	CCWGG	4 cut(s)	946, 973, 1051, 1566
PspCI	CACGTG	1 cut(s)	1231
PspGI	CCWGG	4 cut(s)	946, 973, 1051, 1566
PspN4I	GGNNCC	7 cut(s)	28, 256, 291, 789, 1023, 1072, 1663
PspPI	GGNCC	6 cut(s)	109, 152, 254, 583, 855, 950
PstI	CTGCAG	1 cut(s)	1880
PsuI	RGATCY	4 cut(s)	400, 430, 1201, 1512
PsyI	GACNNNGTC	1 cut(s)	1333
PteI	GCGCGC	1 cut(s)	216
RsaI	GTAC	1 cut(s)	515
RsaNI	GTAC	1 cut(s)	514
RseI	CAYNNNNRTG	6 cut(s)	236, 263, 681, 1182, 1557, 1922
SacI	GAGCTC	2 cut(s)	736, 1566
SapI	GCTCTTC	1 cut(s)	724
SaqAI	TTAA	5 cut(s)	782, 906, 1011, 1088, 1826
SatI	GCNGC	7 cut(s)	127, 474, 504, 558, 1426, 1615, 1876
Sau96I	GGNCC	6 cut(s)	109, 152, 254, 583, 855, 950
SchI	GAGTC	2 cut(s)	5, 1787
ScrFI	CCNGG	7 cut(s)	31, 649, 650, 948, 975, 1053, 1568
SduI	GDGCHC	4 cut(s)	736, 1024, 1554, 1566
SfaNI	GCATC	7 cut(s)	189, 556, 831, 931, 1136, 1174, 1366
SfcI	CTRYAG	2 cut(s)	1419, 1876
SfoI	GGCGCC	2 cut(s)	28, 291
SinI	GGWCC	3 cut(s)	583, 855, 950
SmaI	CCCGGG	1 cut(s)	650
SmiMI	CAYNNNNRTG	6 cut(s)	236, 263, 681, 1182, 1557, 1922
SmlI	CTYRAG	2 cut(s)	404, 1001
SmoI	CTYRAG	2 cut(s)	404, 1001
SsiI	CCGC	5 cut(s)	124, 127, 243, 928, 1425
SspDI	GGCGCC	2 cut(s)	26, 289
SspMI	CTAG	1 cut(s)	1257
SstI	GAGCTC	2 cut(s)	736, 1566
StyD4I	CCNGG	7 cut(s)	29, 647, 648, 946, 973, 1051, 1566
StyI	CCWWGG	2 cut(s)	1256, 1542
TaaI	ACNGT	4 cut(s)	95, 251, 351, 1333
TaiI	ACGT	4 cut(s)	272, 359, 416, 1233
TaqI	TCGA	7 cut(s)	9, 90, 117, 571, 1448, 1454, 1477
TaqII	GACCGA	1 cut(s)	1670
TatI	WGTACW	1 cut(s)	513
TauI	GCSGC	2 cut(s)	129, 1428
TfiI	GAWTC	5 cut(s)	81, 635, 1036, 1153, 1645
Tru1I	TTAA	5 cut(s)	782, 906, 1011, 1088, 1826
Tru9I	TTAA	5 cut(s)	782, 906, 1011, 1088, 1826
TscAI	CASTG	6 cut(s)	238, 256, 991, 1022, 1869, 1929
TseFI	GTSAC	2 cut(s)	61, 1829
TseI	GCWGC	5 cut(s)	473, 503, 557, 1614, 1875
Tsp45I	GTSAC	2 cut(s)	61, 1829
TspDTI	ATGAA	8 cut(s)	188, 395, 705, 791, 1145, 1232, 1743, 1869
TspGWI	ACGGA	2 cut(s)	431, 1332
TspMI	CCCGGG	1 cut(s)	648
TspRI	CASTG	6 cut(s)	238, 256, 991, 1022, 1869, 1929
Tth111I	GACNNNGTC	1 cut(s)	1333
VneI	GTGCAC	1 cut(s)	1550
VpaK11BI	GGWCC	3 cut(s)	583, 855, 950
VspI	ATTAAT	1 cut(s)	1088
XapI	RAATTY	6 cut(s)	157, 659, 778, 920, 1281, 1299
XceI	RCATGY	1 cut(s)	464
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1114, 1195
XmaI	CCCGGG	1 cut(s)	648
XmaJI	CCTAGG	1 cut(s)	1256
XspI	CTAG	1 cut(s)	1257
Zsp2I	ATGCAT	4 cut(s)	105, 380, 898, 1381

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.