Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0007353

Physical Location & Seq

Genomic Coordinates Forward (+)

70250051 .. 70254746

Gene Structure 4696 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG398100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1938 bp

ATGGCCATCGACTCTCTCGTTCTGGGCGCCGGGCAAGAGGTAGGCAAGAGCTGTGTGATTGTGACGATCAACGGGAAACGAATCATGTTCGACTGTGGTATGCATATGGGCCACCTCGACCACCGCCGCTACCCTGACTTCTCCCTCATCGGCCCAAATTTCAATCAAACCCTCACTTGCATCATCATCACTCACTTTCATCTGGATCACATCGGCGCGCTCCCTTACTTCACTGAAGTGTGCGGTTACAGTGGCCCCATTTACATGACGTATCCGACAAAGGCGCTGGCGCCTTTGATGTTGGAGGACTATCGGAAAGTGATGGTTGAGAGAAGGGGCGAGGAGGAACAGTTTACGTCCGAGCATATTGTTGAATGCATGAAGAAAGTTATACCGGTGGATCTCAAGCAGACGTTGCAGGTTGATAAGGATCTTCAAATCCGTGCTTACTATGCCGGACATGTTCTTGGAGCTGCAATGTTTTATGCAAAGGTGGGAGACGCTGCTATGGTGTACACAGGAGACTATAATATGACACCAGACAGGCATCTTGGAGCAGCTCAAATTGATCGACTAAGTTTGGACCTTGTTATTACAGAGTCCACATATGCAACAACCATTCGTGATTCAAAATATGCCCGGGAAAGAGAATTTCTCAGAGCTGTTCACACTTGTGTTGCTGGTGGGGGAAAAGTGCTAATTCCTTCATTTGCTCTGGGAAGAGCTCAGGAACTCTGTATCTTATTAGAAGACTACTGGAAGCGCATGAATTTAAAGGTTCCTATTTACTTCTCAGCAGGTTTGACCCGTCAAGCCAATATGTATTATAAGATGCTCATCAGTTGGACCAGCCAGAAAATCAAAGAAACATACTCCACACATAATGCATTTGATTTTAAAAATGTTCACAAATTTGAGCGGTCTATGATAGATGCTCCTGGACCTTGTGTTCTGTTTGCCACACCAGGGATGATCAGTGGTGGTTTTTCACTTGAGGTTTTTAAGCACTGGGCTCCTTCAGAAAAGAATCTTGTTACAATGCCAGGGTATTGCGTGGCTGGAACCATAGGACATAAATTAATGTCTGGTAAACCGACCAAAATTGATCTGGACAAGGACACCCGAATTGATGTGCGATGCCAGATTCATCAATTGACTTTCAGTCCTCATACAGATGCCAAAGGGATTATGGATCTGGTGAAGTTTCTTTCTCCCAAGCACGTGATACTTGTTCATGGTGAGAAGCCTAGGATGGCTACTCTGAAAGGAAGAATTCAATCCGAACTGGGAATTCAATGCTACGACCCTGCAAACAATGAGACCGTGTCAGTTCCGTCCACCCACTATGCGACAGCAGTAGCTTCCGATGCATTTGTCCAAAGTTGCTCAAATCCAAACTTCAAGTTTTCTACAGGCGGCTCAGAACGCGAACAAGGTTCGAGTTCGAGCAACAGAAAATCCATTCCACGACTGCAAGTAAGTGATGATAGGGTAGCCGAAGGGATCTTGGTTATGGAGAAAAACAAAAAAGCCAAGGTAGTGCACCAAGATGAGCTCCTGGTCATGTTAGAAGAAAAGAAGCACCAAGTCAAGTTTGCATATTGCTGTCCTGCTCACATTGGTAACTTGCAAGAGAATCAAGAGACCGAAGGCACCAGCCTGCCTCTGTCTTCAACAAACAATGATCTGCTTCAGCTATTGTCTGCAAAACTCGCAAATGAACTCTCCGGGGGGAGCATTCAAGATTTCGGGGATCACCTTCAAGTGGAGTCGTTTCGCGTGTCCATTTGTTCAAAGGAGGATTGCCCTTACAGGTTAAGTGACAACGGTGGTGAAGGACACTATGAATTACCAGTGTTCTTTTGCTGCAGTTGGGCAATGGAAGATGAGAAGCTTGCATGGAAAGTCATCTCAGCGTGTCAAGAGTCAACACTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000741 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0009553 GO:0009559 GO:0009561 GO:0009987 GO:0010197 GO:0016043 GO:0030154 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Beta-Casp Lactamase_B_6 RMMBL zf-RING_2

Protein Analysis

645

Amino Acids

72.1

Weight (kDa)

6.56

Isoelectric Point (pI)

39.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	15 - 181	1.2e-09	Metallo-beta-lactamase superfamily
Anti-Pycsar_Apyc1	PF23023	17 - 182	1.5e-08	Anti-Pycsar protein Apyc1
Lactamase_B_6	PF16661	20 - 189	3.4e-20	Metallo-beta-lactamase superfamily domain
Beta-Casp	PF10996	242 - 360	2.5e-28	Beta-Casp domain
RMMBL	PF07521	375 - 434	9.8e-16	Zn-dependent metallo-hydrolase RNA specificity domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0007353)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01730 AT2G01730
fragaria_vesca	FvH4_4g33520
malus_domestica	MD16G1018300.v1.1
prunus_persica	Prupe.1G333600_v2.0.a1
pyrus_communis	pycom16g01670
rosa_chinensis	RchiOBHm_Chr3g0480131 RchiOBHm_Chr4g0443031
rosa_laevigata	RLG00000005961
rosa_multiflora	Rmu_co8263599.1_g000001 Rmu_sc0006604.1_g000001 Rmu_sc0006604.1_g000004 Rmu_sc0008055.1_g000002 Rmu_sc0011261.1_g000006
rosa_roxburghii	Rroxscaffold_5G00383660
rosa_rugosa	Rorug04G0343900
rosa_samantha	Rh3AG232100 Rh3CG261900 Rh3DG257700 Rh4AG398100 Rh4BG409600 Rh4CG425100 Rh4DG404400
rosa_wichuraiana	Rw3G020840 Rw4G034290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	828
Acc36I	ACCTGC	2 cut(s)	409, 788
AccB1I	GGYRCC	3 cut(s)	26, 289, 1652
AccBSI	CCGCTC	1 cut(s)	919
AccII	CGCG	3 cut(s)	218, 1428, 1779
AciI	CCGC	5 cut(s)	124, 127, 243, 919, 1416
AclWI	GGATC	6 cut(s)	213, 408, 438, 1200, 1511, 1761
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	6 cut(s)	157, 650, 769, 911, 1272, 1290
AcuI	CTGAAG	3 cut(s)	255, 1002, 1676
AcvI	CACGTG	1 cut(s)	1222
AcyI	GRCGYC	2 cut(s)	27, 290
AfaI	GTAC	1 cut(s)	515
AfiI	CCNNNNNNNGG	2 cut(s)	966, 1765
AflIII	ACRYGT	1 cut(s)	460
AgeI	ACCGGT	1 cut(s)	394
AhdI	GACNNNNNGTC	1 cut(s)	1161
AjnI	CCWGG	4 cut(s)	937, 964, 1042, 1557
AleI	CACNNNNGTG	2 cut(s)	236, 672
AloI	GAACNNNNNNTCC	2 cut(s)	933, 965
AluBI	AGCT	9 cut(s)	51, 473, 560, 662, 725, 1361, 1555, 1696, 1894
AluI	AGCT	9 cut(s)	51, 473, 560, 662, 725, 1361, 1555, 1696, 1894
Alw21I	GWGCWC	3 cut(s)	727, 1545, 1557
Alw26I	GTCTC	4 cut(s)	492, 516, 1313, 1637
Alw44I	GTGCAC	1 cut(s)	1541
AlwI	GGATC	6 cut(s)	213, 408, 438, 1200, 1511, 1761
Ama87I	CYCGRG	1 cut(s)	639
AoxI	GGCC	4 cut(s)	3, 109, 151, 253
ApaLI	GTGCAC	1 cut(s)	1541
ApeKI	GCWGC	4 cut(s)	473, 503, 557, 1866
ApoI	RAATTY	6 cut(s)	157, 650, 769, 911, 1272, 1290
AseI	ATTAAT	1 cut(s)	1079
AsiGI	ACCGGT	1 cut(s)	394
AspA2I	CCTAGG	1 cut(s)	1247
AspLEI	GCGC	6 cut(s)	29, 218, 220, 286, 292, 765
AspS9I	GGNCC	6 cut(s)	109, 152, 254, 583, 846, 941
AsuC2I	CCSGG	4 cut(s)	31, 640, 641, 1729
AsuHPI	GGTGA	4 cut(s)	1210, 1250, 1748, 1844
AvaI	CYCGRG	1 cut(s)	639
AvaII	GGWCC	3 cut(s)	583, 846, 941
AvrII	CCTAGG	1 cut(s)	1247
BaeGI	GKGCMC	1 cut(s)	1545
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	3 cut(s)	26, 289, 1652
BanII	GRGCYC	3 cut(s)	727, 1015, 1557
BbrPI	CACGTG	1 cut(s)	1222
BbsI	GAAGAC	2 cut(s)	756, 1662
Bbv12I	GWGCWC	3 cut(s)	727, 1545, 1557
BbvI	GCAGC	4 cut(s)	460, 490, 569, 1853
BccI	CCATC	3 cut(s)	14, 316, 1246
BciT130I	CCWGG	4 cut(s)	939, 966, 1044, 1559
BciVI	GTATCC	1 cut(s)	282
BclI	TGATCA	1 cut(s)	972
BcnI	CCSGG	4 cut(s)	31, 640, 641, 1729
BcoDI	GTCTC	4 cut(s)	492, 516, 1313, 1637
BfaI	CTAG	1 cut(s)	1248
BfmI	CTRYAG	2 cut(s)	1410, 1867
BfoI	RGCGCY	3 cut(s)	30, 287, 293
BfuAI	ACCTGC	2 cut(s)	409, 788
BfuI	GTATCC	1 cut(s)	282
BisI	GCNGC	6 cut(s)	127, 474, 504, 558, 1417, 1867
BlnI	CCTAGG	1 cut(s)	1247
BlsI	GCNGC	6 cut(s)	128, 475, 505, 559, 1418, 1868
Bme1390I	CCNGG	8 cut(s)	31, 640, 641, 939, 966, 1044, 1559, 1729
Bme18I	GGWCC	3 cut(s)	583, 846, 941
BmeRI	GACNNNNNGTC	1 cut(s)	1161
BmeT110I	CYCGRG	1 cut(s)	639
BmgT120I	GGNCC	6 cut(s)	109, 152, 254, 583, 846, 941
BmiI	GGNNCC	7 cut(s)	28, 256, 291, 780, 1014, 1063, 1654
BmrFI	CCNGG	8 cut(s)	31, 640, 641, 939, 966, 1044, 1559, 1729
BmrI	ACTGGG	2 cut(s)	1018, 1295
BmsI	GCATC	7 cut(s)	189, 556, 822, 922, 1127, 1165, 1357
BmuI	ACTGGG	2 cut(s)	1018, 1295
BpiI	GAAGAC	2 cut(s)	756, 1662
BplI	GAGNNNNNCTC	2 cut(s)	639, 671
Bpu10I	CCTNAGC	1 cut(s)	726
BpuEI	CTTGAG	2 cut(s)	389, 1013
BpuMI	CCSGG	4 cut(s)	31, 640, 641, 1729
BsaAI	YACGTR	1 cut(s)	1222
BsaBI	GATNNNNATC	2 cut(s)	429, 836
BsaHI	GRCGYC	2 cut(s)	27, 290
BsaI	GGTCTC	2 cut(s)	1313, 1637
BsaJI	CCNNGG	6 cut(s)	639, 965, 1043, 1247, 1533, 1728
BsaWI	WCCGGW	1 cut(s)	394
BsaXI	ACNNNNNCTCC	2 cut(s)	335, 365
Bsc4I	CCNNNNNNNGG	2 cut(s)	966, 1765
Bse118I	RCCGGY	1 cut(s)	394
Bse1I	ACTGG	4 cut(s)	761, 1013, 1290, 1853
Bse3DI	GCAATG	2 cut(s)	483, 1884
Bse8I	GATNNNNATC	2 cut(s)	429, 836
BseBI	CCWGG	4 cut(s)	939, 966, 1044, 1559
BseDI	CCNNGG	6 cut(s)	639, 965, 1043, 1247, 1533, 1728
BseGI	GGATG	2 cut(s)	975, 1257
BseJI	GATNNNNATC	2 cut(s)	429, 836
BseLI	CCNNNNNNNGG	2 cut(s)	966, 1765
BseMI	GCAATG	2 cut(s)	483, 1884
BseMII	CTCAG	5 cut(s)	670, 740, 807, 1434, 1926
BseNI	ACTGG	4 cut(s)	761, 1013, 1290, 1853
BsePI	GCGCGC	1 cut(s)	216
BseRI	GAGGAG	1 cut(s)	356
BseSI	GKGCMC	1 cut(s)	1545
BseXI	GCAGC	4 cut(s)	460, 490, 569, 1853
Bsh1236I	CGCG	3 cut(s)	218, 1428, 1779
BshFI	GGCC	4 cut(s)	5, 111, 153, 255
BshNI	GGYRCC	3 cut(s)	26, 289, 1652
BshTI	ACCGGT	1 cut(s)	394
BsiHKAI	GWGCWC	3 cut(s)	727, 1545, 1557
BsiHKCI	CYCGRG	1 cut(s)	639
BsiSI	CCGG	5 cut(s)	30, 395, 456, 640, 1728
BslI	CCNNNNNNNGG	2 cut(s)	966, 1765
BsmAI	GTCTC	4 cut(s)	492, 516, 1313, 1637
BsmBI	CGTCTC	1 cut(s)	492
BsmI	GAATGC	2 cut(s)	380, 1737
BsnI	GGCC	4 cut(s)	5, 111, 153, 255
Bso31I	GGTCTC	2 cut(s)	1313, 1637
BsoBI	CYCGRG	1 cut(s)	639
Bsp1286I	GDGCHC	4 cut(s)	727, 1015, 1545, 1557
Bsp1407I	TGTACA	1 cut(s)	513
BspACI	CCGC	5 cut(s)	124, 127, 243, 919, 1416
BspANI	GGCC	4 cut(s)	5, 111, 153, 255
BspCNI	CTCAG	5 cut(s)	669, 739, 806, 1433, 1925
BspFNI	CGCG	3 cut(s)	218, 1428, 1779
BspLI	GGNNCC	7 cut(s)	28, 256, 291, 780, 1014, 1063, 1654
BspMAI	CTGCAG	1 cut(s)	1871
BspMI	ACCTGC	2 cut(s)	409, 788
BspPI	GGATC	6 cut(s)	213, 408, 438, 1200, 1511, 1761
BspQI	GCTCTTC	1 cut(s)	715
BspT107I	GGYRCC	3 cut(s)	26, 289, 1652
BspTNI	GGTCTC	2 cut(s)	1313, 1637
BsrBI	CCGCTC	1 cut(s)	919
BsrDI	GCAATG	2 cut(s)	483, 1884
BsrFI	RCCGGY	1 cut(s)	394
BsrGI	TGTACA	1 cut(s)	513
BsrI	ACTGG	4 cut(s)	761, 1013, 1290, 1853
BssAI	RCCGGY	1 cut(s)	394
BssECI	CCNNGG	6 cut(s)	639, 965, 1043, 1247, 1533, 1728
BssHII	GCGCGC	1 cut(s)	216
BssNI	GRCGYC	2 cut(s)	27, 290
BssT1I	CCWWGG	2 cut(s)	1247, 1533
Bst2UI	CCWGG	4 cut(s)	939, 966, 1044, 1559
Bst4CI	ACNGT	5 cut(s)	95, 251, 351, 1324, 1829
Bst6I	CTCTTC	1 cut(s)	715
BstACI	GRCGYC	2 cut(s)	27, 290
BstAPI	GCANNNNNTGC	1 cut(s)	415
BstAUI	TGTACA	1 cut(s)	513
BstBAI	YACGTR	1 cut(s)	1222
BstC8I	GCNNGC	4 cut(s)	218, 288, 1661, 1896
BstDEI	CTNAG	6 cut(s)	575, 656, 726, 793, 1420, 1912
BstF5I	GGATG	2 cut(s)	975, 1257
BstFNI	CGCG	3 cut(s)	218, 1428, 1779
BstH2I	RGCGCY	3 cut(s)	30, 287, 293
BstHHI	GCGC	6 cut(s)	29, 218, 220, 286, 292, 765
BstMAI	GTCTC	4 cut(s)	492, 516, 1313, 1637
BstMWI	GCNNNNNNNGC	5 cut(s)	415, 452, 1367, 1425, 1712
BstNI	CCWGG	4 cut(s)	939, 966, 1044, 1559
BstNSI	RCATGY	1 cut(s)	464
BstSCI	CCNGG	8 cut(s)	29, 638, 639, 937, 964, 1042, 1557, 1727
BstSFI	CTRYAG	2 cut(s)	1410, 1867
BstSLI	GKGCMC	1 cut(s)	1545
BstUI	CGCG	3 cut(s)	218, 1428, 1779
BstV1I	GCAGC	4 cut(s)	460, 490, 569, 1853
BstV2I	GAAGAC	2 cut(s)	756, 1662
BstX2I	RGATCY	4 cut(s)	400, 430, 1192, 1503
BstYI	RGATCY	4 cut(s)	400, 430, 1192, 1503
BsuI	GTATCC	1 cut(s)	282
BsuRI	GGCC	4 cut(s)	5, 111, 153, 255
BtgZI	GCGATG	1 cut(s)	1150
BtsCI	GGATG	2 cut(s)	975, 1257
BtsIMutI	CAGTG	5 cut(s)	231, 256, 982, 1006, 1860
BveI	ACCTGC	2 cut(s)	409, 788
Cac8I	GCNNGC	4 cut(s)	218, 288, 1661, 1896
CfoI	GCGC	6 cut(s)	29, 218, 220, 286, 292, 765
Cfr10I	RCCGGY	1 cut(s)	394
Cfr13I	GGNCC	6 cut(s)	109, 152, 254, 583, 846, 941
Cfr9I	CCCGGG	1 cut(s)	639
CseI	GACGC	1 cut(s)	509
Csp6I	GTAC	1 cut(s)	514
CspAI	ACCGGT	1 cut(s)	394
CviAII	CATG	8 cut(s)	85, 265, 379, 461, 766, 1235, 1564, 1899
CviQI	GTAC	1 cut(s)	514
DdeI	CTNAG	6 cut(s)	575, 656, 726, 793, 1420, 1912
DinI	GGCGCC	2 cut(s)	28, 291
DraI	TTTAAA	2 cut(s)	774, 898
DriI	GACNNNNNGTC	1 cut(s)	1161
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	1 cut(s)	715
Eam1105I	GACNNNNNGTC	1 cut(s)	1161
EarI	CTCTTC	1 cut(s)	715
Ecl136II	GAGCTC	2 cut(s)	725, 1555
Eco130I	CCWWGG	2 cut(s)	1247, 1533
Eco24I	GRGCYC	3 cut(s)	727, 1015, 1557
Eco31I	GGTCTC	2 cut(s)	1313, 1637
Eco47I	GGWCC	3 cut(s)	583, 846, 941
Eco53kI	GAGCTC	2 cut(s)	725, 1555
Eco57I	CTGAAG	3 cut(s)	255, 1002, 1676
Eco72I	CACGTG	1 cut(s)	1222
Eco88I	CYCGRG	1 cut(s)	639
EcoICRI	GAGCTC	2 cut(s)	725, 1555
EcoRI	GAATTC	2 cut(s)	1272, 1290
EcoRII	CCWGG	4 cut(s)	937, 964, 1042, 1557
EcoT14I	CCWWGG	2 cut(s)	1247, 1533
EcoT22I	ATGCAT	4 cut(s)	105, 380, 889, 1372
EcoT38I	GRGCYC	3 cut(s)	727, 1015, 1557
EgeI	GGCGCC	2 cut(s)	28, 291
EheI	GGCGCC	2 cut(s)	28, 291
ErhI	CCWWGG	2 cut(s)	1247, 1533
Esp3I	CGTCTC	1 cut(s)	492
FaeI	CATG	8 cut(s)	88, 268, 382, 464, 769, 1238, 1567, 1902
FatI	CATG	8 cut(s)	84, 264, 378, 460, 765, 1234, 1563, 1898
FauNDI	CATATG	2 cut(s)	105, 607
FbaI	TGATCA	1 cut(s)	972
Fnu4HI	GCNGC	6 cut(s)	127, 474, 504, 558, 1417, 1867
FokI	GGATG	2 cut(s)	982, 1264
FriOI	GRGCYC	3 cut(s)	727, 1015, 1557
Fsp4HI	GCNGC	6 cut(s)	127, 474, 504, 558, 1417, 1867
FspBI	CTAG	1 cut(s)	1248
GlaI	GCGC	6 cut(s)	28, 217, 219, 285, 291, 764
GluI	GCNGC	6 cut(s)	127, 474, 504, 558, 1417, 1867
HaeII	RGCGCY	3 cut(s)	30, 287, 293
HaeIII	GGCC	4 cut(s)	5, 111, 153, 255
HapII	CCGG	5 cut(s)	30, 395, 456, 640, 1728
HgaI	GACGC	1 cut(s)	509
HhaI	GCGC	6 cut(s)	29, 218, 220, 286, 292, 765
Hin1I	GRCGYC	2 cut(s)	27, 290
Hin1II	CATG	8 cut(s)	88, 268, 382, 464, 769, 1238, 1567, 1902
Hin6I	GCGC	6 cut(s)	27, 216, 218, 284, 290, 763
HinP1I	GCGC	6 cut(s)	27, 216, 218, 284, 290, 763
HincII	GTYRAC	1 cut(s)	1929
HindII	GTYRAC	1 cut(s)	1929
HindIII	AAGCTT	1 cut(s)	1892
HinfI	GANTC	9 cut(s)	11, 81, 599, 626, 1027, 1144, 1636, 1769, 1925
HpaII	CCGG	5 cut(s)	30, 395, 456, 640, 1728
HphI	GGTGA	4 cut(s)	1210, 1250, 1748, 1844
Hpy188I	TCNGA	9 cut(s)	276, 315, 361, 659, 1021, 1263, 1282, 1366, 1423
Hpy188III	TCNNGA	7 cut(s)	203, 623, 728, 1109, 1640, 1742, 1922
HpyAV	CCTTC	7 cut(s)	327, 714, 1026, 1493, 1643, 1769, 1829
HpyCH4III	ACNGT	5 cut(s)	95, 251, 351, 1324, 1829
HpyCH4IV	ACGT	4 cut(s)	269, 356, 413, 1221
HpyF10VI	GCNNNNNNNGC	5 cut(s)	415, 452, 1367, 1425, 1712
HpyF3I	CTNAG	6 cut(s)	575, 656, 726, 793, 1420, 1912
HpySE526I	ACGT	4 cut(s)	269, 356, 413, 1221
Hsp92I	GRCGYC	2 cut(s)	27, 290
Hsp92II	CATG	8 cut(s)	88, 268, 382, 464, 769, 1238, 1567, 1902
HspAI	GCGC	6 cut(s)	27, 216, 218, 284, 290, 763
KasI	GGCGCC	2 cut(s)	26, 289
Ksp22I	TGATCA	1 cut(s)	972
LguI	GCTCTTC	1 cut(s)	715
LmnI	GCTCC	7 cut(s)	225, 470, 554, 940, 1018, 1560, 1734
Lsp1109I	GCAGC	4 cut(s)	460, 490, 569, 1853
LweI	GCATC	7 cut(s)	189, 556, 822, 922, 1127, 1165, 1357
MaeI	CTAG	1 cut(s)	1248
MaeII	ACGT	4 cut(s)	269, 356, 413, 1221
MaeIII	GTNAC	5 cut(s)	61, 245, 1033, 1622, 1820
MbiI	CCGCTC	1 cut(s)	919
MboII	GAAGA	8 cut(s)	394, 425, 732, 761, 1281, 1583, 1662, 1895
MfeI	CAATTG	1 cut(s)	1151
MflI	RGATCY	4 cut(s)	400, 430, 1192, 1503
MhlI	GDGCHC	4 cut(s)	727, 1015, 1545, 1557
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	2 cut(s)	27, 290
MlyI	GAGTC	4 cut(s)	5, 608, 1778, 1934
MmeI	TCCRAC	3 cut(s)	282, 299, 824
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	4 cut(s)	105, 380, 889, 1372
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	5 cut(s)	773, 897, 1002, 1079, 1817
MslI	CAYNNNNRTG	5 cut(s)	236, 263, 672, 1173, 1548
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	5 cut(s)	30, 395, 456, 640, 1728
MspR9I	CCNGG	8 cut(s)	31, 640, 641, 939, 966, 1044, 1559, 1729
MunI	CAATTG	1 cut(s)	1151
Mva1269I	GAATGC	2 cut(s)	380, 1737
MvaI	CCWGG	4 cut(s)	939, 966, 1044, 1559
MvnI	CGCG	3 cut(s)	218, 1428, 1779
MwoI	GCNNNNNNNGC	5 cut(s)	415, 452, 1367, 1425, 1712
NarI	GGCGCC	2 cut(s)	27, 290
NciI	CCSGG	4 cut(s)	31, 640, 641, 1729
NdeI	CATATG	2 cut(s)	105, 607
NlaIII	CATG	8 cut(s)	88, 268, 382, 464, 769, 1238, 1567, 1902
NlaIV	GGNNCC	7 cut(s)	28, 256, 291, 780, 1014, 1063, 1654
NmuCI	GTSAC	2 cut(s)	61, 1820
NsiI	ATGCAT	4 cut(s)	105, 380, 889, 1372
NspI	RCATGY	1 cut(s)	464
OliI	CACNNNNGTG	2 cut(s)	236, 672
PauI	GCGCGC	1 cut(s)	216
PciI	ACATGT	1 cut(s)	460
PciSI	GCTCTTC	1 cut(s)	715
PcsI	WCGNNNNNNNCGW	1 cut(s)	15
PctI	GAATGC	2 cut(s)	380, 1737
PfeI	GAWTC	5 cut(s)	81, 626, 1027, 1144, 1636
PflFI	GACNNNGTC	1 cut(s)	1324
PfoI	TCCNGGA	1 cut(s)	937
PinAI	ACCGGT	1 cut(s)	394
PkrI	GCNGC	6 cut(s)	128, 475, 505, 559, 1418, 1868
PleI	GAGTC	4 cut(s)	5, 607, 1777, 1933
PluTI	GGCGCC	2 cut(s)	30, 293
PmaCI	CACGTG	1 cut(s)	1222
PmlI	CACGTG	1 cut(s)	1222
PpsI	GAGTC	4 cut(s)	5, 607, 1777, 1933
Ppu21I	YACGTR	1 cut(s)	1222
PscI	ACATGT	1 cut(s)	460
PshBI	ATTAAT	1 cut(s)	1079
PsiI	TTATAA	1 cut(s)	828
Psp124BI	GAGCTC	2 cut(s)	727, 1557
Psp6I	CCWGG	4 cut(s)	937, 964, 1042, 1557
PspCI	CACGTG	1 cut(s)	1222
PspGI	CCWGG	4 cut(s)	937, 964, 1042, 1557
PspN4I	GGNNCC	7 cut(s)	28, 256, 291, 780, 1014, 1063, 1654
PspPI	GGNCC	6 cut(s)	109, 152, 254, 583, 846, 941
PstI	CTGCAG	1 cut(s)	1871
PsuI	RGATCY	4 cut(s)	400, 430, 1192, 1503
PsyI	GACNNNGTC	1 cut(s)	1324
PteI	GCGCGC	1 cut(s)	216
RsaI	GTAC	1 cut(s)	515
RsaNI	GTAC	1 cut(s)	514
RseI	CAYNNNNRTG	5 cut(s)	236, 263, 672, 1173, 1548
SacI	GAGCTC	2 cut(s)	727, 1557
SapI	GCTCTTC	1 cut(s)	715
SaqAI	TTAA	5 cut(s)	773, 897, 1002, 1079, 1817
SatI	GCNGC	6 cut(s)	127, 474, 504, 558, 1417, 1867
Sau96I	GGNCC	6 cut(s)	109, 152, 254, 583, 846, 941
SchI	GAGTC	4 cut(s)	5, 608, 1778, 1934
ScrFI	CCNGG	8 cut(s)	31, 640, 641, 939, 966, 1044, 1559, 1729
SduI	GDGCHC	4 cut(s)	727, 1015, 1545, 1557
SfaNI	GCATC	7 cut(s)	189, 556, 822, 922, 1127, 1165, 1357
SfcI	CTRYAG	2 cut(s)	1410, 1867
SfoI	GGCGCC	2 cut(s)	28, 291
SinI	GGWCC	3 cut(s)	583, 846, 941
SmaI	CCCGGG	1 cut(s)	641
SmiMI	CAYNNNNRTG	5 cut(s)	236, 263, 672, 1173, 1548
SmlI	CTYRAG	2 cut(s)	404, 992
SmoI	CTYRAG	2 cut(s)	404, 992
SsiI	CCGC	5 cut(s)	124, 127, 243, 919, 1416
SspDI	GGCGCC	2 cut(s)	26, 289
SspMI	CTAG	1 cut(s)	1248
SstI	GAGCTC	2 cut(s)	727, 1557
StyD4I	CCNGG	8 cut(s)	29, 638, 639, 937, 964, 1042, 1557, 1727
StyI	CCWWGG	2 cut(s)	1247, 1533
TaaI	ACNGT	5 cut(s)	95, 251, 351, 1324, 1829
TaiI	ACGT	4 cut(s)	272, 359, 416, 1224
TaqI	TCGA	6 cut(s)	9, 90, 117, 571, 1439, 1445
TaqII	GACCGA	1 cut(s)	1661
TatI	WGTACW	1 cut(s)	513
TauI	GCSGC	2 cut(s)	129, 1419
TfiI	GAWTC	5 cut(s)	81, 626, 1027, 1144, 1636
Tru1I	TTAA	5 cut(s)	773, 897, 1002, 1079, 1817
Tru9I	TTAA	5 cut(s)	773, 897, 1002, 1079, 1817
TscAI	CASTG	5 cut(s)	238, 256, 982, 1013, 1860
TseFI	GTSAC	2 cut(s)	61, 1820
TseI	GCWGC	4 cut(s)	473, 503, 557, 1866
Tsp45I	GTSAC	2 cut(s)	61, 1820
TspDTI	ATGAA	8 cut(s)	188, 395, 696, 782, 1136, 1223, 1734, 1860
TspGWI	ACGGA	2 cut(s)	431, 1323
TspMI	CCCGGG	1 cut(s)	639
TspRI	CASTG	5 cut(s)	238, 256, 982, 1013, 1860
Tth111I	GACNNNGTC	1 cut(s)	1324
VneI	GTGCAC	1 cut(s)	1541
VpaK11BI	GGWCC	3 cut(s)	583, 846, 941
VspI	ATTAAT	1 cut(s)	1079
XapI	RAATTY	6 cut(s)	157, 650, 769, 911, 1272, 1290
XceI	RCATGY	1 cut(s)	464
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1105, 1186
XmaI	CCCGGG	1 cut(s)	639
XmaJI	CCTAGG	1 cut(s)	1247
XspI	CTAG	1 cut(s)	1248
Zsp2I	ATGCAT	4 cut(s)	105, 380, 889, 1372

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.