Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0007353

Physical Location & Seq

Genomic Coordinates Reverse (-)

3081994 .. 3086738

Gene Structure 4745 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005961

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1944 bp

ATGGCCATCGACTCTCTCGTTCTGGGCGCCGGGCAAGAGGTAGGCAAGAGCTGTGTGATTGTGACGATCAACGGGAAACGAATCATGTTCGACTGTGGTATGCATATGGGCCACCTCGACCACCGCCGCTACCCTGACTTCTCCCTCATCGGCCCAAATTTCAATCAAACCCTCACTTGCATCATCATCACTCACTTTCATCTGGATCACATCGGCGCGCTCCCTTACTTCACTGAAGTCTGCGGTTACAGTGGCCCCATTTACATGACGTATCCGACGAAGGCGCTGGCGCCTTTGATGTTGGAGGACTATCGGAAAGTGATGGTTGAGAGAAGGGGCGAGGAGGAACAGTTTACGTCCGAGCATATTGTTGAATGCATGAAGAAAGTTATACCGGTGGATCTGAAGCAGACGGTGCAGGTTGATAAGGATCTTCAAATCCGTGCTTACTACGCCGGACATGTTCTTGGAGCTGCAATGTTTTATGCAAAGGTGGGAGACGCTGCTATGGTGTACACAGGAGACTATAATATGACACCAGATAGGCATCTTGGAGCGGCTCAAATTGATCGACTAAGTTTGGACCTTGTTATTACAGAGTCCACTTATGCAACAACCATTCGTGATTCAAAATATGCCCGGGAAAGAGAATTTCTCAGAGCTTTAGATTGCATGCTCGCGTGTTATAACTCTAATAACTTAATCAGCATTGAAGCCTTTCAAGCTGAACTCTGTATCTTATTGGAAGACTACTGGGAGCGCATGAATTTAAAGGTTCCTATTTACTTCTCAGCAGGTTTGACCCGTCAAGCCAATATGTATTATAAGATGCTCATCAGTTGGACCAGCCAGAAAATCAAAGAAACATACTCCACACATAATGCATTTGATTTTAAAAATGTTCACAAATTTGAGCGGTCTATGATAGATGCTCCTGGACCTTGTGTTCTGTTTGCCACACCAGGAATGATCAGTGGTGGTTTTTCACTTGAGGTTTTTAAGCACTGGGCTCCTTTGGAAAAGAATCTTGTTACAATGCCAGGGTATTGCGTGGCTGGAACCATAGGACATAAATTAATGTCTGGTAAACCGACCAAAATTGATCTGGACAAGGACACCCGAATTGATGTGCGATGCCAGATTCATCAATTGACTTTCAGTCCTCATACAGATGCCAAAGGGATTATGGATCTGGTGAAGTTTCTTTCTCCCAAGCACGTGATACTTGTTCATGGTGAGAAGCCTAGGATGGCTACTCTGAAAGGAAGAATTCAATCCGAACTGGGAATTCAATGCTACGTCCCTGCAAACAATGAGACTGTGTCAGTTCCGTCCACCCACTATGCGACAGCAGTAGCTTCCGATGCATTTGTCCAAAGTTGCTCAAATCCAAACTTCAAGTTTTCTACAGGCGGCTCAGAACACGAACAAGGTTCAAGTTCGAGTAACAGAAAATCCATTCCACGACTGCAAGTAAGTGATGATAGGGTAGCCGAAGGGATCTTGGTTATGGAGAAAAACAAAAAAGCCAAGGTAGTGCACCAAGATGAGCTCCTGGTCATGTTAGACGAAAAGAAGCACCAAGTCAAGTTTGCATATTGCTGTCCTGCTCACATTGGTAACTTGCAAGAGAATCAAGAGACCGAAAGAACCGGCCTGCCTCTGTCTTCAACAAACAATGATCTGCTTCGGCTATTGTCTGCAAAACTCGCAAATGAACTCTCTGAGGGGAACATTCAAGATTTCGGGGATCACCTTCAAGTGGAGTCGTTTCGCGTGTCCATTTGTTCAAAGGAGGATTGCCCTTACAGATTAAGTGACAACGGTGGTGAAGAACACTATGAACACTTTGAACACTATGAACACTTTTGCTGCAGTTGGGCAATGGAAGATGAGAAGCTTGCATGGAAAGTCATCTCAGTGTGTCAAAAATCAACACTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000741 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0009553 GO:0009559 GO:0009561 GO:0009987 GO:0010197 GO:0016043 GO:0030154 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Beta-Casp Lactamase_B_6 RMMBL zf-RING_2

Protein Analysis

648

Amino Acids

73.04

Weight (kDa)

6.2

Isoelectric Point (pI)

37.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	15 - 181	1.2e-09	Metallo-beta-lactamase superfamily
Anti-Pycsar_Apyc1	PF23023	17 - 182	1.1e-08	Anti-Pycsar protein Apyc1
Lactamase_B_6	PF16661	20 - 189	2.1e-20	Metallo-beta-lactamase superfamily domain
Lactamase_B_2	PF12706	27 - 197	7.9e-06	Beta-lactamase superfamily domain
Beta-Casp	PF10996	243 - 359	3.4e-26	Beta-Casp domain
RMMBL	PF07521	374 - 435	6.8e-17	Zn-dependent metallo-hydrolase RNA specificity domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007353)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01730 AT2G01730
fragaria_vesca	FvH4_4g33520
malus_domestica	MD16G1018300.v1.1
prunus_persica	Prupe.1G333600_v2.0.a1
pyrus_communis	pycom16g01670
rosa_chinensis	RchiOBHm_Chr3g0480131 RchiOBHm_Chr4g0443031
rosa_laevigata	RLG00000005961
rosa_multiflora	Rmu_co8263599.1_g000001 Rmu_sc0006604.1_g000001 Rmu_sc0006604.1_g000004 Rmu_sc0008055.1_g000002 Rmu_sc0011261.1_g000006
rosa_roxburghii	Rroxscaffold_5G00383660
rosa_rugosa	Rorug04G0343900
rosa_samantha	Rh3AG232100 Rh3CG261900 Rh3DG257700 Rh4AG398100 Rh4BG409600 Rh4CG425100 Rh4DG404400
rosa_wichuraiana	Rw3G020840 Rw4G034290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	687, 825
Acc36I	ACCTGC	2 cut(s)	409, 785
AccB1I	GGYRCC	2 cut(s)	26, 289
AccBSI	CCGCTC	2 cut(s)	557, 916
AccII	CGCG	3 cut(s)	218, 680, 1776
AciI	CCGC	6 cut(s)	124, 127, 243, 557, 916, 1413
AclWI	GGATC	6 cut(s)	213, 408, 438, 1197, 1508, 1758
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	6 cut(s)	157, 650, 766, 908, 1269, 1287
AcuI	CTGAAG	2 cut(s)	255, 425
AcvI	CACGTG	1 cut(s)	1219
AcyI	GRCGYC	2 cut(s)	27, 290
AfaI	GTAC	1 cut(s)	515
AfiI	CCNNNNNNNGG	1 cut(s)	1762
AflIII	ACRYGT	1 cut(s)	460
AgeI	ACCGGT	1 cut(s)	394
AhdI	GACNNNNNGTC	1 cut(s)	1158
AjnI	CCWGG	4 cut(s)	934, 961, 1039, 1554
AloI	GAACNNNNNNTCC	2 cut(s)	930, 962
AluBI	AGCT	7 cut(s)	51, 473, 662, 725, 1358, 1552, 1900
AluI	AGCT	7 cut(s)	51, 473, 662, 725, 1358, 1552, 1900
Alw21I	GWGCWC	2 cut(s)	1542, 1554
Alw26I	GTCTC	4 cut(s)	492, 516, 1310, 1634
Alw44I	GTGCAC	1 cut(s)	1538
AlwI	GGATC	6 cut(s)	213, 408, 438, 1197, 1508, 1758
Ama87I	CYCGRG	1 cut(s)	639
AoxI	GGCC	5 cut(s)	3, 109, 151, 253, 1654
ApaLI	GTGCAC	1 cut(s)	1538
ApeKI	GCWGC	3 cut(s)	473, 503, 1872
ApoI	RAATTY	6 cut(s)	157, 650, 766, 908, 1269, 1287
AseI	ATTAAT	1 cut(s)	1076
AsiGI	ACCGGT	1 cut(s)	394
Asp700I	GAANNNNTTC	1 cut(s)	717
AspA2I	CCTAGG	1 cut(s)	1244
AspLEI	GCGC	6 cut(s)	29, 218, 220, 286, 292, 762
AspS9I	GGNCC	6 cut(s)	109, 152, 254, 583, 843, 938
AsuC2I	CCSGG	3 cut(s)	31, 640, 641
AsuHPI	GGTGA	4 cut(s)	1207, 1247, 1745, 1841
AvaI	CYCGRG	1 cut(s)	639
AvaII	GGWCC	3 cut(s)	583, 843, 938
AvrII	CCTAGG	1 cut(s)	1244
BaeGI	GKGCMC	1 cut(s)	1542
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	2 cut(s)	26, 289
BanII	GRGCYC	2 cut(s)	1012, 1554
BbrPI	CACGTG	1 cut(s)	1219
BbsI	GAAGAC	2 cut(s)	753, 1659
Bbv12I	GWGCWC	2 cut(s)	1542, 1554
BbvI	GCAGC	3 cut(s)	460, 490, 1859
BccI	CCATC	3 cut(s)	14, 316, 1243
BciT130I	CCWGG	4 cut(s)	936, 963, 1041, 1556
BciVI	GTATCC	1 cut(s)	282
BclI	TGATCA	1 cut(s)	969
BcnI	CCSGG	3 cut(s)	31, 640, 641
BcoDI	GTCTC	4 cut(s)	492, 516, 1310, 1634
BfaI	CTAG	1 cut(s)	1245
BfmI	CTRYAG	2 cut(s)	1407, 1873
BfoI	RGCGCY	3 cut(s)	30, 287, 293
BfuAI	ACCTGC	2 cut(s)	409, 785
BfuI	GTATCC	1 cut(s)	282
BisI	GCNGC	6 cut(s)	127, 474, 504, 558, 1414, 1873
BlnI	CCTAGG	1 cut(s)	1244
BlsI	GCNGC	6 cut(s)	128, 475, 505, 559, 1415, 1874
Bme1390I	CCNGG	7 cut(s)	31, 640, 641, 936, 963, 1041, 1556
Bme18I	GGWCC	3 cut(s)	583, 843, 938
BmeRI	GACNNNNNGTC	1 cut(s)	1158
BmeT110I	CYCGRG	1 cut(s)	639
BmgT120I	GGNCC	6 cut(s)	109, 152, 254, 583, 843, 938
BmiI	GGNNCC	6 cut(s)	28, 256, 291, 777, 1011, 1060
BmrFI	CCNGG	7 cut(s)	31, 640, 641, 936, 963, 1041, 1556
BmrI	ACTGGG	3 cut(s)	763, 1015, 1292
BmsI	GCATC	7 cut(s)	189, 556, 819, 919, 1124, 1162, 1354
BmuI	ACTGGG	3 cut(s)	763, 1015, 1292
BpiI	GAAGAC	2 cut(s)	753, 1659
BplI	GAGNNNNNCTC	2 cut(s)	639, 671
BpuEI	CTTGAG	1 cut(s)	1010
BpuMI	CCSGG	3 cut(s)	31, 640, 641
BsaAI	YACGTR	1 cut(s)	1219
BsaBI	GATNNNNATC	3 cut(s)	429, 546, 833
BsaHI	GRCGYC	2 cut(s)	27, 290
BsaI	GGTCTC	1 cut(s)	1634
BsaJI	CCNNGG	4 cut(s)	639, 1040, 1244, 1530
BsaWI	WCCGGW	1 cut(s)	394
BsaXI	ACNNNNNCTCC	2 cut(s)	335, 365
Bsc4I	CCNNNNNNNGG	1 cut(s)	1762
Bse118I	RCCGGY	2 cut(s)	394, 1652
Bse1I	ACTGG	3 cut(s)	758, 1010, 1287
Bse3DI	GCAATG	2 cut(s)	483, 1890
Bse8I	GATNNNNATC	3 cut(s)	429, 546, 833
BseBI	CCWGG	4 cut(s)	936, 963, 1041, 1556
BseDI	CCNNGG	4 cut(s)	639, 1040, 1244, 1530
BseGI	GGATG	1 cut(s)	1254
BseJI	GATNNNNATC	3 cut(s)	429, 546, 833
BseLI	CCNNNNNNNGG	1 cut(s)	1762
BseMI	GCAATG	2 cut(s)	483, 1890
BseMII	CTCAG	5 cut(s)	670, 804, 1431, 1716, 1932
BseNI	ACTGG	3 cut(s)	758, 1010, 1287
BsePI	GCGCGC	1 cut(s)	216
BseRI	GAGGAG	1 cut(s)	356
BseSI	GKGCMC	1 cut(s)	1542
BseXI	GCAGC	3 cut(s)	460, 490, 1859
BsgI	GTGCAG	1 cut(s)	437
Bsh1236I	CGCG	3 cut(s)	218, 680, 1776
BshFI	GGCC	5 cut(s)	5, 111, 153, 255, 1656
BshNI	GGYRCC	2 cut(s)	26, 289
BshTI	ACCGGT	1 cut(s)	394
BsiHKAI	GWGCWC	2 cut(s)	1542, 1554
BsiHKCI	CYCGRG	1 cut(s)	639
BsiSI	CCGG	5 cut(s)	30, 395, 456, 640, 1653
BslFI	GGGAC	1 cut(s)	1286
BslI	CCNNNNNNNGG	1 cut(s)	1762
BsmAI	GTCTC	4 cut(s)	492, 516, 1310, 1634
BsmBI	CGTCTC	1 cut(s)	492
BsmFI	GGGAC	1 cut(s)	1286
BsmI	GAATGC	1 cut(s)	380
BsnI	GGCC	5 cut(s)	5, 111, 153, 255, 1656
Bso31I	GGTCTC	1 cut(s)	1634
BsoBI	CYCGRG	1 cut(s)	639
Bsp1286I	GDGCHC	3 cut(s)	1012, 1542, 1554
Bsp1407I	TGTACA	1 cut(s)	513
BspACI	CCGC	6 cut(s)	124, 127, 243, 557, 916, 1413
BspANI	GGCC	5 cut(s)	5, 111, 153, 255, 1656
BspCNI	CTCAG	5 cut(s)	669, 803, 1430, 1717, 1931
BspFNI	CGCG	3 cut(s)	218, 680, 1776
BspLI	GGNNCC	6 cut(s)	28, 256, 291, 777, 1011, 1060
BspMAI	CTGCAG	1 cut(s)	1877
BspMI	ACCTGC	2 cut(s)	409, 785
BspPI	GGATC	6 cut(s)	213, 408, 438, 1197, 1508, 1758
BspT107I	GGYRCC	2 cut(s)	26, 289
BspTNI	GGTCTC	1 cut(s)	1634
BsrBI	CCGCTC	2 cut(s)	557, 916
BsrDI	GCAATG	2 cut(s)	483, 1890
BsrFI	RCCGGY	2 cut(s)	394, 1652
BsrGI	TGTACA	1 cut(s)	513
BsrI	ACTGG	3 cut(s)	758, 1010, 1287
BssAI	RCCGGY	2 cut(s)	394, 1652
BssECI	CCNNGG	4 cut(s)	639, 1040, 1244, 1530
BssHII	GCGCGC	1 cut(s)	216
BssNI	GRCGYC	2 cut(s)	27, 290
BssT1I	CCWWGG	2 cut(s)	1244, 1530
Bst2UI	CCWGG	4 cut(s)	936, 963, 1041, 1556
Bst4CI	ACNGT	6 cut(s)	95, 251, 351, 415, 1321, 1826
BstACI	GRCGYC	2 cut(s)	27, 290
BstAPI	GCANNNNNTGC	1 cut(s)	415
BstAUI	TGTACA	1 cut(s)	513
BstBAI	YACGTR	1 cut(s)	1219
BstC8I	GCNNGC	6 cut(s)	218, 288, 674, 678, 1658, 1902
BstDEI	CTNAG	6 cut(s)	575, 656, 790, 1417, 1725, 1918
BstF5I	GGATG	1 cut(s)	1254
BstFNI	CGCG	3 cut(s)	218, 680, 1776
BstH2I	RGCGCY	3 cut(s)	30, 287, 293
BstHHI	GCGC	6 cut(s)	29, 218, 220, 286, 292, 762
BstMAI	GTCTC	4 cut(s)	492, 516, 1310, 1634
BstMWI	GCNNNNNNNGC	5 cut(s)	415, 452, 722, 1364, 1709
BstNI	CCWGG	4 cut(s)	936, 963, 1041, 1556
BstNSI	RCATGY	2 cut(s)	464, 676
BstSCI	CCNGG	7 cut(s)	29, 638, 639, 934, 961, 1039, 1554
BstSFI	CTRYAG	2 cut(s)	1407, 1873
BstSLI	GKGCMC	1 cut(s)	1542
BstUI	CGCG	3 cut(s)	218, 680, 1776
BstV1I	GCAGC	3 cut(s)	460, 490, 1859
BstV2I	GAAGAC	2 cut(s)	753, 1659
BstX2I	RGATCY	4 cut(s)	400, 430, 1189, 1500
BstYI	RGATCY	4 cut(s)	400, 430, 1189, 1500
BsuI	GTATCC	1 cut(s)	282
BsuRI	GGCC	5 cut(s)	5, 111, 153, 255, 1656
BtgZI	GCGATG	1 cut(s)	1147
BtsCI	GGATG	1 cut(s)	1254
BtsIMutI	CAGTG	5 cut(s)	231, 256, 979, 1003, 1926
BveI	ACCTGC	2 cut(s)	409, 785
Cac8I	GCNNGC	6 cut(s)	218, 288, 674, 678, 1658, 1902
CfoI	GCGC	6 cut(s)	29, 218, 220, 286, 292, 762
Cfr10I	RCCGGY	2 cut(s)	394, 1652
Cfr13I	GGNCC	6 cut(s)	109, 152, 254, 583, 843, 938
Cfr9I	CCCGGG	1 cut(s)	639
CseI	GACGC	1 cut(s)	509
Csp6I	GTAC	1 cut(s)	514
CspAI	ACCGGT	1 cut(s)	394
CviAII	CATG	9 cut(s)	85, 265, 379, 461, 673, 763, 1232, 1561, 1905
CviQI	GTAC	1 cut(s)	514
DdeI	CTNAG	6 cut(s)	575, 656, 790, 1417, 1725, 1918
DinI	GGCGCC	2 cut(s)	28, 291
DraI	TTTAAA	2 cut(s)	771, 895
DriI	GACNNNNNGTC	1 cut(s)	1158
EaeI	YGGCCR	1 cut(s)	3
Eam1105I	GACNNNNNGTC	1 cut(s)	1158
Ecl136II	GAGCTC	1 cut(s)	1552
Eco130I	CCWWGG	2 cut(s)	1244, 1530
Eco24I	GRGCYC	2 cut(s)	1012, 1554
Eco31I	GGTCTC	1 cut(s)	1634
Eco47I	GGWCC	3 cut(s)	583, 843, 938
Eco53kI	GAGCTC	1 cut(s)	1552
Eco57I	CTGAAG	2 cut(s)	255, 425
Eco72I	CACGTG	1 cut(s)	1219
Eco88I	CYCGRG	1 cut(s)	639
EcoICRI	GAGCTC	1 cut(s)	1552
EcoRI	GAATTC	2 cut(s)	1269, 1287
EcoRII	CCWGG	4 cut(s)	934, 961, 1039, 1554
EcoT14I	CCWWGG	2 cut(s)	1244, 1530
EcoT22I	ATGCAT	4 cut(s)	105, 380, 886, 1369
EcoT38I	GRGCYC	2 cut(s)	1012, 1554
EgeI	GGCGCC	2 cut(s)	28, 291
EheI	GGCGCC	2 cut(s)	28, 291
ErhI	CCWWGG	2 cut(s)	1244, 1530
Esp3I	CGTCTC	1 cut(s)	492
FaeI	CATG	9 cut(s)	88, 268, 382, 464, 676, 766, 1235, 1564, 1908
FaqI	GGGAC	1 cut(s)	1286
FatI	CATG	9 cut(s)	84, 264, 378, 460, 672, 762, 1231, 1560, 1904
FauNDI	CATATG	1 cut(s)	105
FbaI	TGATCA	1 cut(s)	969
Fnu4HI	GCNGC	6 cut(s)	127, 474, 504, 558, 1414, 1873
FokI	GGATG	1 cut(s)	1261
FriOI	GRGCYC	2 cut(s)	1012, 1554
Fsp4HI	GCNGC	6 cut(s)	127, 474, 504, 558, 1414, 1873
FspBI	CTAG	1 cut(s)	1245
GlaI	GCGC	6 cut(s)	28, 217, 219, 285, 291, 761
GluI	GCNGC	6 cut(s)	127, 474, 504, 558, 1414, 1873
HaeII	RGCGCY	3 cut(s)	30, 287, 293
HaeIII	GGCC	5 cut(s)	5, 111, 153, 255, 1656
HapII	CCGG	5 cut(s)	30, 395, 456, 640, 1653
HgaI	GACGC	1 cut(s)	509
HhaI	GCGC	6 cut(s)	29, 218, 220, 286, 292, 762
Hin1I	GRCGYC	2 cut(s)	27, 290
Hin1II	CATG	9 cut(s)	88, 268, 382, 464, 676, 766, 1235, 1564, 1908
Hin6I	GCGC	6 cut(s)	27, 216, 218, 284, 290, 760
HinP1I	GCGC	6 cut(s)	27, 216, 218, 284, 290, 760
HindIII	AAGCTT	1 cut(s)	1898
HinfI	GANTC	8 cut(s)	11, 81, 599, 626, 1024, 1141, 1633, 1766
HpaII	CCGG	5 cut(s)	30, 395, 456, 640, 1653
HphI	GGTGA	4 cut(s)	1207, 1247, 1745, 1841
Hpy166II	GTNNAC	8 cut(s)	354, 514, 516, 603, 904, 1088, 1335, 1540
Hpy188III	TCNNGA	5 cut(s)	203, 623, 1106, 1637, 1739
Hpy8I	GTNNAC	8 cut(s)	354, 514, 516, 603, 904, 1088, 1335, 1540
Hpy99I	CGWCG	1 cut(s)	280
HpyAV	CCTTC	4 cut(s)	274, 327, 1490, 1766
HpyCH4III	ACNGT	6 cut(s)	95, 251, 351, 415, 1321, 1826
HpyCH4IV	ACGT	4 cut(s)	269, 356, 1218, 1299
HpyF10VI	GCNNNNNNNGC	5 cut(s)	415, 452, 722, 1364, 1709
HpyF3I	CTNAG	6 cut(s)	575, 656, 790, 1417, 1725, 1918
HpySE526I	ACGT	4 cut(s)	269, 356, 1218, 1299
Hsp92I	GRCGYC	2 cut(s)	27, 290
Hsp92II	CATG	9 cut(s)	88, 268, 382, 464, 676, 766, 1235, 1564, 1908
HspAI	GCGC	6 cut(s)	27, 216, 218, 284, 290, 760
KasI	GGCGCC	2 cut(s)	26, 289
Ksp22I	TGATCA	1 cut(s)	969
LmnI	GCTCC	7 cut(s)	225, 470, 554, 757, 937, 1015, 1557
Lsp1109I	GCAGC	3 cut(s)	460, 490, 1859
LweI	GCATC	7 cut(s)	189, 556, 819, 919, 1124, 1162, 1354
MaeI	CTAG	1 cut(s)	1245
MaeII	ACGT	4 cut(s)	269, 356, 1218, 1299
MaeIII	GTNAC	6 cut(s)	61, 245, 1030, 1445, 1619, 1817
MbiI	CCGCTC	2 cut(s)	557, 916
MboII	GAAGA	7 cut(s)	394, 425, 758, 1278, 1659, 1844, 1901
MfeI	CAATTG	1 cut(s)	1148
MflI	RGATCY	4 cut(s)	400, 430, 1189, 1500
MhlI	GDGCHC	3 cut(s)	1012, 1542, 1554
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	2 cut(s)	27, 290
MlyI	GAGTC	3 cut(s)	5, 608, 1775
MmeI	TCCRAC	3 cut(s)	282, 299, 821
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	4 cut(s)	105, 380, 886, 1369
MroXI	GAANNNNTTC	1 cut(s)	717
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	6 cut(s)	701, 770, 894, 999, 1076, 1814
MslI	CAYNNNNRTG	4 cut(s)	263, 1170, 1545, 1919
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	5 cut(s)	30, 395, 456, 640, 1653
MspR9I	CCNGG	7 cut(s)	31, 640, 641, 936, 963, 1041, 1556
MunI	CAATTG	1 cut(s)	1148
Mva1269I	GAATGC	1 cut(s)	380
MvaI	CCWGG	4 cut(s)	936, 963, 1041, 1556
MvnI	CGCG	3 cut(s)	218, 680, 1776
MwoI	GCNNNNNNNGC	5 cut(s)	415, 452, 722, 1364, 1709
NarI	GGCGCC	2 cut(s)	27, 290
NciI	CCSGG	3 cut(s)	31, 640, 641
NdeI	CATATG	1 cut(s)	105
NlaIII	CATG	9 cut(s)	88, 268, 382, 464, 676, 766, 1235, 1564, 1908
NlaIV	GGNNCC	6 cut(s)	28, 256, 291, 777, 1011, 1060
NmuCI	GTSAC	2 cut(s)	61, 1817
NsiI	ATGCAT	4 cut(s)	105, 380, 886, 1369
NspI	RCATGY	2 cut(s)	464, 676
PaeI	GCATGC	1 cut(s)	676
PauI	GCGCGC	1 cut(s)	216
PciI	ACATGT	1 cut(s)	460
PcsI	WCGNNNNNNNCGW	2 cut(s)	15, 275
PctI	GAATGC	1 cut(s)	380
PdmI	GAANNNNTTC	1 cut(s)	717
PfeI	GAWTC	5 cut(s)	81, 626, 1024, 1141, 1633
PflFI	GACNNNGTC	1 cut(s)	1321
PfoI	TCCNGGA	1 cut(s)	934
PinAI	ACCGGT	1 cut(s)	394
PkrI	GCNGC	6 cut(s)	128, 475, 505, 559, 1415, 1874
PleI	GAGTC	3 cut(s)	5, 607, 1774
PluTI	GGCGCC	2 cut(s)	30, 293
PmaCI	CACGTG	1 cut(s)	1219
PmlI	CACGTG	1 cut(s)	1219
PpsI	GAGTC	3 cut(s)	5, 607, 1774
Ppu21I	YACGTR	1 cut(s)	1219
PscI	ACATGT	1 cut(s)	460
PshBI	ATTAAT	1 cut(s)	1076
PsiI	TTATAA	2 cut(s)	687, 825
Psp124BI	GAGCTC	1 cut(s)	1554
Psp6I	CCWGG	4 cut(s)	934, 961, 1039, 1554
PspCI	CACGTG	1 cut(s)	1219
PspGI	CCWGG	4 cut(s)	934, 961, 1039, 1554
PspN4I	GGNNCC	6 cut(s)	28, 256, 291, 777, 1011, 1060
PspPI	GGNCC	6 cut(s)	109, 152, 254, 583, 843, 938
PstI	CTGCAG	1 cut(s)	1877
PsuI	RGATCY	4 cut(s)	400, 430, 1189, 1500
PsyI	GACNNNGTC	1 cut(s)	1321
PteI	GCGCGC	1 cut(s)	216
RsaI	GTAC	1 cut(s)	515
RsaNI	GTAC	1 cut(s)	514
RseI	CAYNNNNRTG	4 cut(s)	263, 1170, 1545, 1919
SacI	GAGCTC	1 cut(s)	1554
SaqAI	TTAA	6 cut(s)	701, 770, 894, 999, 1076, 1814
SatI	GCNGC	6 cut(s)	127, 474, 504, 558, 1414, 1873
Sau96I	GGNCC	6 cut(s)	109, 152, 254, 583, 843, 938
SchI	GAGTC	3 cut(s)	5, 608, 1775
ScrFI	CCNGG	7 cut(s)	31, 640, 641, 936, 963, 1041, 1556
SduI	GDGCHC	3 cut(s)	1012, 1542, 1554
SfaNI	GCATC	7 cut(s)	189, 556, 819, 919, 1124, 1162, 1354
SfcI	CTRYAG	2 cut(s)	1407, 1873
SfoI	GGCGCC	2 cut(s)	28, 291
SinI	GGWCC	3 cut(s)	583, 843, 938
SmaI	CCCGGG	1 cut(s)	641
SmiMI	CAYNNNNRTG	4 cut(s)	263, 1170, 1545, 1919
SmlI	CTYRAG	1 cut(s)	989
SmoI	CTYRAG	1 cut(s)	989
SphI	GCATGC	1 cut(s)	676
SsiI	CCGC	6 cut(s)	124, 127, 243, 557, 916, 1413
SspDI	GGCGCC	2 cut(s)	26, 289
SspMI	CTAG	1 cut(s)	1245
SstI	GAGCTC	1 cut(s)	1554
StyD4I	CCNGG	7 cut(s)	29, 638, 639, 934, 961, 1039, 1554
StyI	CCWWGG	2 cut(s)	1244, 1530
TaaI	ACNGT	6 cut(s)	95, 251, 351, 415, 1321, 1826
TaiI	ACGT	4 cut(s)	272, 359, 1221, 1302
TaqI	TCGA	5 cut(s)	9, 90, 117, 571, 1442
TaqII	GACCGA	1 cut(s)	1658
TatI	WGTACW	1 cut(s)	513
TauI	GCSGC	3 cut(s)	129, 560, 1416
TfiI	GAWTC	5 cut(s)	81, 626, 1024, 1141, 1633
Tru1I	TTAA	6 cut(s)	701, 770, 894, 999, 1076, 1814
Tru9I	TTAA	6 cut(s)	701, 770, 894, 999, 1076, 1814
TscAI	CASTG	5 cut(s)	238, 256, 979, 1010, 1926
TseFI	GTSAC	2 cut(s)	61, 1817
TseI	GCWGC	3 cut(s)	473, 503, 1872
Tsp45I	GTSAC	2 cut(s)	61, 1817
TspDTI	ATGAA	8 cut(s)	188, 395, 779, 1133, 1220, 1731, 1857, 1875
TspGWI	ACGGA	2 cut(s)	431, 1320
TspMI	CCCGGG	1 cut(s)	639
TspRI	CASTG	5 cut(s)	238, 256, 979, 1010, 1926
Tth111I	GACNNNGTC	1 cut(s)	1321
VneI	GTGCAC	1 cut(s)	1538
VpaK11BI	GGWCC	3 cut(s)	583, 843, 938
VspI	ATTAAT	1 cut(s)	1076
XapI	RAATTY	6 cut(s)	157, 650, 766, 908, 1269, 1287
XceI	RCATGY	2 cut(s)	464, 676
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1102, 1183
XmaI	CCCGGG	1 cut(s)	639
XmaJI	CCTAGG	1 cut(s)	1244
XmnI	GAANNNNTTC	1 cut(s)	717
XspI	CTAG	1 cut(s)	1245
Zsp2I	ATGCAT	4 cut(s)	105, 380, 886, 1369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.