RchiOBHm_Chr4g0443031

Cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007353
Physical Location & Seq
Forward (+)
64375459 .. 64380689
5231 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41075

Sequence Viewer

Length: 2061 bp
ATGGCCATCGACTCTCTCGTTCTGGGCGCCGGGCAAGAGGTAGGCAAGAGCTGTGTGATTGTGACGATCAACGGGAAACGAATCATGTTCGACTGTGGTATGCATATGGGCCACCTCGACCACCGCCGCTACCCTGACTTCTCCCTCATCGGCCCAAATTTCAATCAAACCCTCACTTGCATCATCATCACTCACTTTCATCTGGATCACATCGGCGCGCTCCCTTACTTCACTGAAGTGTGCGGTTACAGTGGCCCCATTTACATGACGTATCCGACAAAGGCGCTGGCGCCTTTGATGTTGGAGGACTATCGGAAAGTGATGGTTGAGAGAAGGGGCGAGGAGGAACAGTTTACGTCCGAGCATATTGTTGAATGCATGAAGAAAGTTATACCGGTGGATCTCAAGCAGACGTTGCAGGTTGATAAGGATCTTCAAATCCGTGCTTACTATGCCGGACATGTTCTTGGAGCTGCAATGTTTTATGCAAAGGTGGGAGACGCTGCTATGGTGTACACAGGAGACTATAATATGACACCAGACAGGCATCTTGGAGCAGCTCAAATTGATCGACTAAGTTTGGACCTTGTTATTACAGAATTCTGTTTTCTTTACCTTATTTCGAGGAACATATGTATCAGATGTTTATCCTTTGTTGTGGTGACACCACAATCCTTGATGCTTCATCATCTTTCCTACATTAGAGATTGCTTCAAGTGCGTGTCCACATATGCAACAACCATTCGTGATTCAAAATATGCCCGGGAAAGAGAATTTCTCAGAGCTGTTCACACTTGTGTTGCTGGTGGGGGAAAAGTGCTAATTCCTTCATTTGCTCTGGGAAGAGCTCAGGAACTCTGTATCTTATTAGAAGACTACTGGAAGCGCATGAATTTAAAGGTTCCTATTTACTTCTCAGCAGGTTTGACCCGTCAAGCCAATATGTATTATAAGATGCTCATCAGTTGGACCAGCCAGAAAATCAAAGAAACATACTCCACACATAATGCATTTGATTTTAAAAATGTTCACAAATTTGAGCGGTCTATGATAGATGCTCCTGGACCTTGTGTTCTGTTTGCCACACCAGGGATGATCAGTGGTGGTTTTTCACTTGAGGTTTTTAAGCACTGGGCTCCTTCAGAAAAGAATCTTGTTACAATGCCAGGGTATTGCGTGGCTGGAACCATAGGACATAAATTAATGTCTGGTAAACCGACCAAAATTGATCTGGACAAGGACACCCGAATTGATGTGCGATGCCAGATTCATCAATTGACTTTCAGTCCTCATACAGATGCCAAAGGGATTATGGATCTGGTGAAGTTTCTTTCTCCCAAGCACGTGATACTTGTTCATGGTGAGAAGCCTAGGATGGCTACTCTGAAAGGAAGAATTCAATCCGAACTGGGAATTCAATGCTACGACCCTGCAAACAATGAGACCGTGTCAGTTCCGTCCACCCACTATGCGACAGCAGTAGCTTCCGATGCATTTGTCCAAAGTTGCTCAAATCCAAACTTCAAGTTTTCTACAGGCGGCTCAGAACGCGAACAAGGTTCGAGTTCGAGCAACAGAAAATCCATTCCACGACTGCAAGTAAGTGATGATAGGGTAGCCGAAGGGATCTTGGTTATGGAGAAAAACAAAAAAGCCAAGGTAGTGCACCAAGATGAGCTCCTGGTCATGTTAGAAGAAAAGAAGCACCAAGTCAAGTTTGCATATTGCTGTCCTGCTCACATTGGTAACTTGCAAGAGAATCAAGAGACCGAAGGCACCAGCCTGCCTCTGTCTTCAACAAACAATGATCTGCTTCAGCTATTGTCTGCAAAACTCGCAAATGAACTCTCCGGGGGGAGCATTCAAGATTTCGGGGATCACCTTCAAGTGGAGTCGTTTCGCGTGTCCATTTGTTCAAAGGAGGATTGCCCTTACAGGTTAAGTGACAACGGTGGTGAAGGACACTATGAATTACCAGTGTTCTTTTGCTGCAGTTGGGCAATGGAAGATGAGAAGCTTGCATGGAAAGTCATCTCAGCGTGTCAAGAGTCAACACTTTGA

Protein Analysis

686

Amino Acids

76.97

Weight (kDa)

6.94

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 15 - 178 1.5e-09 Metallo-beta-lactamase superfamily
Anti-Pycsar_Apyc1 PF23023 17 - 182 1.7e-08 Anti-Pycsar protein Apyc1
Lactamase_B_6 PF16661 20 - 189 4.3e-20 Metallo-beta-lactamase superfamily domain
Beta-Casp PF10996 283 - 401 2.8e-28 Beta-Casp domain
RMMBL PF07521 416 - 475 1.1e-15 Zn-dependent metallo-hydrolase RNA specificity domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 951
Acc36I ACCTGC 2 cut(s) 409, 911
AccB1I GGYRCC 3 cut(s) 26, 289, 1775
AccBSI CCGCTC 1 cut(s) 1042
AccII CGCG 3 cut(s) 218, 1551, 1902
AciI CCGC 5 cut(s) 124, 127, 243, 1042, 1539
AclWI GGATC 6 cut(s) 213, 408, 438, 1323, 1634, 1884
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 7 cut(s) 157, 599, 773, 892, 1034, 1395, 1413
AcuI CTGAAG 3 cut(s) 255, 1125, 1799
AcvI CACGTG 1 cut(s) 1345
AcyI GRCGYC 2 cut(s) 27, 290
AfaI GTAC 1 cut(s) 515
AfiI CCNNNNNNNGG 2 cut(s) 1089, 1888
AflIII ACRYGT 1 cut(s) 460
AgeI ACCGGT 1 cut(s) 394
AhdI GACNNNNNGTC 1 cut(s) 1284
AjnI CCWGG 4 cut(s) 1060, 1087, 1165, 1680
AleI CACNNNNGTG 2 cut(s) 236, 795
AloI GAACNNNNNNTCC 2 cut(s) 1056, 1088
AluBI AGCT 9 cut(s) 51, 473, 560, 785, 848, 1484, 1678, 1819, 2017
AluI AGCT 9 cut(s) 51, 473, 560, 785, 848, 1484, 1678, 1819, 2017
Alw21I GWGCWC 3 cut(s) 850, 1668, 1680
Alw26I GTCTC 4 cut(s) 492, 516, 1436, 1760
Alw44I GTGCAC 1 cut(s) 1664
AlwI GGATC 6 cut(s) 213, 408, 438, 1323, 1634, 1884
Ama87I CYCGRG 1 cut(s) 762
AoxI GGCC 4 cut(s) 3, 109, 151, 253
ApaLI GTGCAC 1 cut(s) 1664
ApeKI GCWGC 4 cut(s) 473, 503, 557, 1989
ApoI RAATTY 7 cut(s) 157, 599, 773, 892, 1034, 1395, 1413
AseI ATTAAT 1 cut(s) 1202
AsiGI ACCGGT 1 cut(s) 394
AspA2I CCTAGG 1 cut(s) 1370
AspLEI GCGC 6 cut(s) 29, 218, 220, 286, 292, 888
AspS9I GGNCC 6 cut(s) 109, 152, 254, 583, 969, 1064
AsuC2I CCSGG 4 cut(s) 31, 763, 764, 1852
AsuHPI GGTGA 5 cut(s) 673, 1333, 1373, 1871, 1967
AvaI CYCGRG 1 cut(s) 762
AvaII GGWCC 3 cut(s) 583, 969, 1064
AvrII CCTAGG 1 cut(s) 1370
BaeGI GKGCMC 1 cut(s) 1668
BaeI ACNNNNGTAYC 2 cut(s) 619, 652
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 3 cut(s) 26, 289, 1775
BanII GRGCYC 3 cut(s) 850, 1138, 1680
BbrPI CACGTG 1 cut(s) 1345
BbsI GAAGAC 2 cut(s) 879, 1785
Bbv12I GWGCWC 3 cut(s) 850, 1668, 1680
BbvI GCAGC 4 cut(s) 460, 490, 569, 1976
BccI CCATC 3 cut(s) 14, 316, 1369
BciT130I CCWGG 4 cut(s) 1062, 1089, 1167, 1682
BciVI GTATCC 1 cut(s) 282
BclI TGATCA 1 cut(s) 1095
BcnI CCSGG 4 cut(s) 31, 763, 764, 1852
BcoDI GTCTC 4 cut(s) 492, 516, 1436, 1760
BfaI CTAG 1 cut(s) 1371
BfmI CTRYAG 2 cut(s) 1533, 1990
BfoI RGCGCY 3 cut(s) 30, 287, 293
BfuAI ACCTGC 2 cut(s) 409, 911
BfuI GTATCC 1 cut(s) 282
BisI GCNGC 6 cut(s) 127, 474, 504, 558, 1540, 1990
BlnI CCTAGG 1 cut(s) 1370
BlsI GCNGC 6 cut(s) 128, 475, 505, 559, 1541, 1991
Bme1390I CCNGG 8 cut(s) 31, 763, 764, 1062, 1089, 1167, 1682, 1852
Bme18I GGWCC 3 cut(s) 583, 969, 1064
BmeRI GACNNNNNGTC 1 cut(s) 1284
BmeT110I CYCGRG 1 cut(s) 762
BmgT120I GGNCC 6 cut(s) 109, 152, 254, 583, 969, 1064
BmiI GGNNCC 7 cut(s) 28, 256, 291, 903, 1137, 1186, 1777
BmrFI CCNGG 8 cut(s) 31, 763, 764, 1062, 1089, 1167, 1682, 1852
BmrI ACTGGG 2 cut(s) 1141, 1418
BmsI GCATC 8 cut(s) 189, 556, 669, 945, 1045, 1250, 1288, 1480
BmuI ACTGGG 2 cut(s) 1141, 1418
BpiI GAAGAC 2 cut(s) 879, 1785
BplI GAGNNNNNCTC 2 cut(s) 762, 794
Bpu10I CCTNAGC 1 cut(s) 849
BpuEI CTTGAG 2 cut(s) 389, 1136
BpuMI CCSGG 4 cut(s) 31, 763, 764, 1852
BsaAI YACGTR 1 cut(s) 1345
BsaBI GATNNNNATC 3 cut(s) 429, 646, 959
BsaHI GRCGYC 2 cut(s) 27, 290
BsaI GGTCTC 2 cut(s) 1436, 1760
BsaJI CCNNGG 6 cut(s) 762, 1088, 1166, 1370, 1656, 1851
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 2 cut(s) 1089, 1888
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 4 cut(s) 884, 1136, 1413, 1976
Bse3DI GCAATG 2 cut(s) 483, 2007
Bse8I GATNNNNATC 3 cut(s) 429, 646, 959
BseBI CCWGG 4 cut(s) 1062, 1089, 1167, 1682
BseDI CCNNGG 6 cut(s) 762, 1088, 1166, 1370, 1656, 1851
BseGI GGATG 2 cut(s) 1098, 1380
BseJI GATNNNNATC 3 cut(s) 429, 646, 959
BseLI CCNNNNNNNGG 2 cut(s) 1089, 1888
BseMI GCAATG 2 cut(s) 483, 2007
BseMII CTCAG 5 cut(s) 793, 863, 930, 1557, 2049
BseNI ACTGG 4 cut(s) 884, 1136, 1413, 1976
BsePI GCGCGC 1 cut(s) 216
BseRI GAGGAG 1 cut(s) 356
BseSI GKGCMC 1 cut(s) 1668
BseXI GCAGC 4 cut(s) 460, 490, 569, 1976
Bsh1236I CGCG 3 cut(s) 218, 1551, 1902
BshFI GGCC 4 cut(s) 5, 111, 153, 255
BshNI GGYRCC 3 cut(s) 26, 289, 1775
BshTI ACCGGT 1 cut(s) 394
BsiHKAI GWGCWC 3 cut(s) 850, 1668, 1680
BsiHKCI CYCGRG 1 cut(s) 762
BsiSI CCGG 5 cut(s) 30, 395, 456, 763, 1851
BslI CCNNNNNNNGG 2 cut(s) 1089, 1888
BsmAI GTCTC 4 cut(s) 492, 516, 1436, 1760
BsmBI CGTCTC 1 cut(s) 492
BsmI GAATGC 2 cut(s) 380, 1860
BsnI GGCC 4 cut(s) 5, 111, 153, 255
Bso31I GGTCTC 2 cut(s) 1436, 1760
BsoBI CYCGRG 1 cut(s) 762
Bsp1286I GDGCHC 4 cut(s) 850, 1138, 1668, 1680
Bsp1407I TGTACA 1 cut(s) 513
BspACI CCGC 5 cut(s) 124, 127, 243, 1042, 1539
BspANI GGCC 4 cut(s) 5, 111, 153, 255
BspCNI CTCAG 5 cut(s) 792, 862, 929, 1556, 2048
BspFNI CGCG 3 cut(s) 218, 1551, 1902
BspLI GGNNCC 7 cut(s) 28, 256, 291, 903, 1137, 1186, 1777
BspMAI CTGCAG 1 cut(s) 1994
BspMI ACCTGC 2 cut(s) 409, 911
BspPI GGATC 6 cut(s) 213, 408, 438, 1323, 1634, 1884
BspQI GCTCTTC 1 cut(s) 838
BspT107I GGYRCC 3 cut(s) 26, 289, 1775
BspTNI GGTCTC 2 cut(s) 1436, 1760
BsrBI CCGCTC 1 cut(s) 1042
BsrDI GCAATG 2 cut(s) 483, 2007
BsrFI RCCGGY 1 cut(s) 394
BsrGI TGTACA 1 cut(s) 513
BsrI ACTGG 4 cut(s) 884, 1136, 1413, 1976
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 6 cut(s) 762, 1088, 1166, 1370, 1656, 1851
BssHII GCGCGC 1 cut(s) 216
BssNI GRCGYC 2 cut(s) 27, 290
BssT1I CCWWGG 2 cut(s) 1370, 1656
Bst2UI CCWGG 4 cut(s) 1062, 1089, 1167, 1682
Bst4CI ACNGT 5 cut(s) 95, 251, 351, 1447, 1952
Bst6I CTCTTC 1 cut(s) 838
BstACI GRCGYC 2 cut(s) 27, 290
BstAPI GCANNNNNTGC 1 cut(s) 415
BstAUI TGTACA 1 cut(s) 513
BstBAI YACGTR 1 cut(s) 1345
BstC8I GCNNGC 4 cut(s) 218, 288, 1784, 2019
BstDEI CTNAG 6 cut(s) 575, 779, 849, 916, 1543, 2035
BstF5I GGATG 2 cut(s) 1098, 1380
BstFNI CGCG 3 cut(s) 218, 1551, 1902
BstH2I RGCGCY 3 cut(s) 30, 287, 293
BstHHI GCGC 6 cut(s) 29, 218, 220, 286, 292, 888
BstMAI GTCTC 4 cut(s) 492, 516, 1436, 1760
BstMWI GCNNNNNNNGC 6 cut(s) 415, 452, 717, 1490, 1548, 1835
BstNI CCWGG 4 cut(s) 1062, 1089, 1167, 1682
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 8 cut(s) 29, 761, 762, 1060, 1087, 1165, 1680, 1850
BstSFI CTRYAG 2 cut(s) 1533, 1990
BstSLI GKGCMC 1 cut(s) 1668
BstUI CGCG 3 cut(s) 218, 1551, 1902
BstV1I GCAGC 4 cut(s) 460, 490, 569, 1976
BstV2I GAAGAC 2 cut(s) 879, 1785
BstX2I RGATCY 4 cut(s) 400, 430, 1315, 1626
BstYI RGATCY 4 cut(s) 400, 430, 1315, 1626
BsuI GTATCC 1 cut(s) 282
BsuRI GGCC 4 cut(s) 5, 111, 153, 255
BtgZI GCGATG 1 cut(s) 1273
BtsCI GGATG 2 cut(s) 1098, 1380
BtsIMutI CAGTG 5 cut(s) 231, 256, 1105, 1129, 1983
BveI ACCTGC 2 cut(s) 409, 911
Cac8I GCNNGC 4 cut(s) 218, 288, 1784, 2019
CfoI GCGC 6 cut(s) 29, 218, 220, 286, 292, 888
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 6 cut(s) 109, 152, 254, 583, 969, 1064
Cfr9I CCCGGG 1 cut(s) 762
CseI GACGC 1 cut(s) 509
Csp6I GTAC 1 cut(s) 514
CspAI ACCGGT 1 cut(s) 394
CspCI CAANNNNNGTGG 2 cut(s) 657, 692
CviAII CATG 8 cut(s) 85, 265, 379, 461, 889, 1358, 1687, 2022
CviQI GTAC 1 cut(s) 514
DdeI CTNAG 6 cut(s) 575, 779, 849, 916, 1543, 2035
DinI GGCGCC 2 cut(s) 28, 291
DraI TTTAAA 2 cut(s) 897, 1021
DriI GACNNNNNGTC 1 cut(s) 1284
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 838
Eam1105I GACNNNNNGTC 1 cut(s) 1284
EarI CTCTTC 1 cut(s) 838
Ecl136II GAGCTC 2 cut(s) 848, 1678
Eco130I CCWWGG 2 cut(s) 1370, 1656
Eco24I GRGCYC 3 cut(s) 850, 1138, 1680
Eco31I GGTCTC 2 cut(s) 1436, 1760
Eco47I GGWCC 3 cut(s) 583, 969, 1064
Eco53kI GAGCTC 2 cut(s) 848, 1678
Eco57I CTGAAG 3 cut(s) 255, 1125, 1799
Eco72I CACGTG 1 cut(s) 1345
Eco88I CYCGRG 1 cut(s) 762
EcoICRI GAGCTC 2 cut(s) 848, 1678
EcoRI GAATTC 3 cut(s) 599, 1395, 1413
EcoRII CCWGG 4 cut(s) 1060, 1087, 1165, 1680
EcoT14I CCWWGG 2 cut(s) 1370, 1656
EcoT22I ATGCAT 4 cut(s) 105, 380, 1012, 1495
EcoT38I GRGCYC 3 cut(s) 850, 1138, 1680
EgeI GGCGCC 2 cut(s) 28, 291
EheI GGCGCC 2 cut(s) 28, 291
ErhI CCWWGG 2 cut(s) 1370, 1656
Esp3I CGTCTC 1 cut(s) 492
FaeI CATG 8 cut(s) 88, 268, 382, 464, 892, 1361, 1690, 2025
FatI CATG 8 cut(s) 84, 264, 378, 460, 888, 1357, 1686, 2021
FauNDI CATATG 3 cut(s) 105, 632, 730
FbaI TGATCA 1 cut(s) 1095
Fnu4HI GCNGC 6 cut(s) 127, 474, 504, 558, 1540, 1990
FokI GGATG 2 cut(s) 1105, 1387
FriOI GRGCYC 3 cut(s) 850, 1138, 1680
Fsp4HI GCNGC 6 cut(s) 127, 474, 504, 558, 1540, 1990
FspBI CTAG 1 cut(s) 1371
GlaI GCGC 6 cut(s) 28, 217, 219, 285, 291, 887
GluI GCNGC 6 cut(s) 127, 474, 504, 558, 1540, 1990
HaeII RGCGCY 3 cut(s) 30, 287, 293
HaeIII GGCC 4 cut(s) 5, 111, 153, 255
HapII CCGG 5 cut(s) 30, 395, 456, 763, 1851
HgaI GACGC 1 cut(s) 509
HhaI GCGC 6 cut(s) 29, 218, 220, 286, 292, 888
Hin1I GRCGYC 2 cut(s) 27, 290
Hin1II CATG 8 cut(s) 88, 268, 382, 464, 892, 1361, 1690, 2025
Hin6I GCGC 6 cut(s) 27, 216, 218, 284, 290, 886
HinP1I GCGC 6 cut(s) 27, 216, 218, 284, 290, 886
HincII GTYRAC 1 cut(s) 2052
HindII GTYRAC 1 cut(s) 2052
HindIII AAGCTT 1 cut(s) 2015
HinfI GANTC 8 cut(s) 11, 81, 749, 1150, 1267, 1759, 1892, 2048
HpaII CCGG 5 cut(s) 30, 395, 456, 763, 1851
HphI GGTGA 5 cut(s) 673, 1333, 1373, 1871, 1967
Hpy188III TCNNGA 7 cut(s) 203, 746, 851, 1232, 1763, 1865, 2045
HpyAV CCTTC 7 cut(s) 327, 837, 1149, 1616, 1766, 1892, 1952
HpyCH4III ACNGT 5 cut(s) 95, 251, 351, 1447, 1952
HpyCH4IV ACGT 4 cut(s) 269, 356, 413, 1344
HpyF10VI GCNNNNNNNGC 6 cut(s) 415, 452, 717, 1490, 1548, 1835
HpyF3I CTNAG 6 cut(s) 575, 779, 849, 916, 1543, 2035
HpySE526I ACGT 4 cut(s) 269, 356, 413, 1344
Hsp92I GRCGYC 2 cut(s) 27, 290
Hsp92II CATG 8 cut(s) 88, 268, 382, 464, 892, 1361, 1690, 2025
HspAI GCGC 6 cut(s) 27, 216, 218, 284, 290, 886
KasI GGCGCC 2 cut(s) 26, 289
Ksp22I TGATCA 1 cut(s) 1095
LguI GCTCTTC 1 cut(s) 838
LmnI GCTCC 7 cut(s) 225, 470, 554, 1063, 1141, 1683, 1857
Lsp1109I GCAGC 4 cut(s) 460, 490, 569, 1976
LweI GCATC 8 cut(s) 189, 556, 669, 945, 1045, 1250, 1288, 1480
MaeI CTAG 1 cut(s) 1371
MaeII ACGT 4 cut(s) 269, 356, 413, 1344
MaeIII GTNAC 6 cut(s) 61, 245, 661, 1156, 1745, 1943
MbiI CCGCTC 1 cut(s) 1042
MboII GAAGA 8 cut(s) 394, 425, 855, 884, 1404, 1706, 1785, 2018
MfeI CAATTG 1 cut(s) 1274
MflI RGATCY 4 cut(s) 400, 430, 1315, 1626
MhlI GDGCHC 4 cut(s) 850, 1138, 1668, 1680
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 2 cut(s) 27, 290
MlyI GAGTC 3 cut(s) 5, 1901, 2057
MmeI TCCRAC 3 cut(s) 282, 299, 947
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 4 cut(s) 105, 380, 1012, 1495
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 896, 1020, 1125, 1202, 1940
MslI CAYNNNNRTG 5 cut(s) 236, 263, 795, 1296, 1671
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 5 cut(s) 30, 395, 456, 763, 1851
MspR9I CCNGG 8 cut(s) 31, 763, 764, 1062, 1089, 1167, 1682, 1852
MunI CAATTG 1 cut(s) 1274
Mva1269I GAATGC 2 cut(s) 380, 1860
MvaI CCWGG 4 cut(s) 1062, 1089, 1167, 1682
MvnI CGCG 3 cut(s) 218, 1551, 1902
MwoI GCNNNNNNNGC 6 cut(s) 415, 452, 717, 1490, 1548, 1835
NarI GGCGCC 2 cut(s) 27, 290
NciI CCSGG 4 cut(s) 31, 763, 764, 1852
NdeI CATATG 3 cut(s) 105, 632, 730
NlaIII CATG 8 cut(s) 88, 268, 382, 464, 892, 1361, 1690, 2025
NlaIV GGNNCC 7 cut(s) 28, 256, 291, 903, 1137, 1186, 1777
NmuCI GTSAC 3 cut(s) 61, 661, 1943
NsiI ATGCAT 4 cut(s) 105, 380, 1012, 1495
NspI RCATGY 1 cut(s) 464
OliI CACNNNNGTG 2 cut(s) 236, 795
PauI GCGCGC 1 cut(s) 216
PciI ACATGT 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 838
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 2 cut(s) 380, 1860
PfeI GAWTC 5 cut(s) 81, 749, 1150, 1267, 1759
PflFI GACNNNGTC 1 cut(s) 1447
PfoI TCCNGGA 1 cut(s) 1060
PinAI ACCGGT 1 cut(s) 394
PkrI GCNGC 6 cut(s) 128, 475, 505, 559, 1541, 1991
PleI GAGTC 3 cut(s) 5, 1900, 2056
PluTI GGCGCC 2 cut(s) 30, 293
PmaCI CACGTG 1 cut(s) 1345
PmlI CACGTG 1 cut(s) 1345
PpsI GAGTC 3 cut(s) 5, 1900, 2056
Ppu21I YACGTR 1 cut(s) 1345
PscI ACATGT 1 cut(s) 460
PshBI ATTAAT 1 cut(s) 1202
PsiI TTATAA 1 cut(s) 951
Psp124BI GAGCTC 2 cut(s) 850, 1680
Psp6I CCWGG 4 cut(s) 1060, 1087, 1165, 1680
PspCI CACGTG 1 cut(s) 1345
PspGI CCWGG 4 cut(s) 1060, 1087, 1165, 1680
PspN4I GGNNCC 7 cut(s) 28, 256, 291, 903, 1137, 1186, 1777
PspPI GGNCC 6 cut(s) 109, 152, 254, 583, 969, 1064
PstI CTGCAG 1 cut(s) 1994
PsuI RGATCY 4 cut(s) 400, 430, 1315, 1626
PsyI GACNNNGTC 1 cut(s) 1447
PteI GCGCGC 1 cut(s) 216
RsaI GTAC 1 cut(s) 515
RsaNI GTAC 1 cut(s) 514
RseI CAYNNNNRTG 5 cut(s) 236, 263, 795, 1296, 1671
SacI GAGCTC 2 cut(s) 850, 1680
SapI GCTCTTC 1 cut(s) 838
SaqAI TTAA 5 cut(s) 896, 1020, 1125, 1202, 1940
SatI GCNGC 6 cut(s) 127, 474, 504, 558, 1540, 1990
Sau96I GGNCC 6 cut(s) 109, 152, 254, 583, 969, 1064
SchI GAGTC 3 cut(s) 5, 1901, 2057
ScrFI CCNGG 8 cut(s) 31, 763, 764, 1062, 1089, 1167, 1682, 1852
SduI GDGCHC 4 cut(s) 850, 1138, 1668, 1680
SfaNI GCATC 8 cut(s) 189, 556, 669, 945, 1045, 1250, 1288, 1480
SfcI CTRYAG 2 cut(s) 1533, 1990
SfoI GGCGCC 2 cut(s) 28, 291
SinI GGWCC 3 cut(s) 583, 969, 1064
SmaI CCCGGG 1 cut(s) 764
SmiMI CAYNNNNRTG 5 cut(s) 236, 263, 795, 1296, 1671
SmlI CTYRAG 2 cut(s) 404, 1115
SmoI CTYRAG 2 cut(s) 404, 1115
SsiI CCGC 5 cut(s) 124, 127, 243, 1042, 1539
SspDI GGCGCC 2 cut(s) 26, 289
SspMI CTAG 1 cut(s) 1371
SstI GAGCTC 2 cut(s) 850, 1680
StyD4I CCNGG 8 cut(s) 29, 761, 762, 1060, 1087, 1165, 1680, 1850
StyI CCWWGG 2 cut(s) 1370, 1656
TaaI ACNGT 5 cut(s) 95, 251, 351, 1447, 1952
TaiI ACGT 4 cut(s) 272, 359, 416, 1347
TaqI TCGA 7 cut(s) 9, 90, 117, 571, 623, 1562, 1568
TaqII GACCGA 1 cut(s) 1784
TatI WGTACW 1 cut(s) 513
TauI GCSGC 2 cut(s) 129, 1542
TfiI GAWTC 5 cut(s) 81, 749, 1150, 1267, 1759
Tru1I TTAA 5 cut(s) 896, 1020, 1125, 1202, 1940
Tru9I TTAA 5 cut(s) 896, 1020, 1125, 1202, 1940
TscAI CASTG 5 cut(s) 238, 256, 1105, 1136, 1983
TseFI GTSAC 3 cut(s) 61, 661, 1943
TseI GCWGC 4 cut(s) 473, 503, 557, 1989
Tsp45I GTSAC 3 cut(s) 61, 661, 1943
TspDTI ATGAA 9 cut(s) 188, 395, 674, 819, 905, 1259, 1346, 1857, 1983
TspGWI ACGGA 2 cut(s) 431, 1446
TspMI CCCGGG 1 cut(s) 762
TspRI CASTG 5 cut(s) 238, 256, 1105, 1136, 1983
Tth111I GACNNNGTC 1 cut(s) 1447
VneI GTGCAC 1 cut(s) 1664
VpaK11BI GGWCC 3 cut(s) 583, 969, 1064
VspI ATTAAT 1 cut(s) 1202
XapI RAATTY 7 cut(s) 157, 599, 773, 892, 1034, 1395, 1413
XceI RCATGY 1 cut(s) 464
XcmI CCANNNNNNNNNTGG 2 cut(s) 1228, 1309
XmaI CCCGGG 1 cut(s) 762
XmaJI CCTAGG 1 cut(s) 1370
XspI CTAG 1 cut(s) 1371
Zsp2I ATGCAT 4 cut(s) 105, 380, 1012, 1495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.