Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007353

Physical Location & Seq

Genomic Coordinates Forward (+)

1336645 .. 1341107

Gene Structure 4463 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1018300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGGCCATCGACTCTCTCGTCCTCGGCGCCGGGCAAGAGGTCGGTAAGAGCTGCGTGGTGGTGACAATCAATGGGAAACGAATCATGTTCGACTGCGGAATGCACATGGGCTATCTCGACCACCGCCGCTACCCCGATTTCTCTCGCATTCCCAAATCGCAGAACGAGTCGAACTTCGATCACTCCATTACTTGCATCATCATCACCCACTTTCACTTAGATCACGTTGGGGCTCTTCCTTACTTCACCGAGGTCTGCGGTTATCAGGGTCCAATTTACATGACGTATCCGACAAAGGCACTGTCTCCTATAATGTTGGAGGACTATCGAAAAGTGATGGTGGAGCGAAGGGGTGAGGAAGAGCAGTTTTCGTCTCATCACATTGCCGAGTGCATGAAGAAAGTAATACCTGTGGATCTGAAGCAGACAGTGCAGGTTGATAAAGACCTTCAAATCCGTGCATACTATGCAGGACATGTTCTTGGAGCGGCAATGTTTTACGCAAAGGTGGGAGACGCCACTATGGTGTACACAGGAGACTATAATATGACACCAGATAGGCATCTTGGAGCAGCTCAAATTGACCGACTAAATTTGGACCTTCTTATTTCAGAATCCACGTATGCAACAACCATACGCGATTCAAAATATGCCCGGGAAAGAGAATTTCTCAGAGCTGTTCATAAATGTGTTGCTGCCGGTGGAAAAGTTCTAATTCCAACTTTTGCTCTTGGAAGAGCTCAGGAACTCTGTATCTTGTTGGAAGATTACTGGGAGCGCATGAATCTAAAGGTTCCTATTTACTTCTCAGCAGGTTTGACCCTTCAAGCCAATATGTATTATAAGATGCTTATCAGTTGGACCAGTCAGAAAGTCAAAGAAACGTACTCCACGCATAATGCCTTTGATTTTAAGAACGCTCACAAGTTCGACCGTTCGATGATGCATGCTCCTGGACCCTGTGTTCTGTTTGCGACACCAGGGATGATCAGTGGTGGCTTTTCACTTGAGGTTTTCAAGCACTGGGCTCCTTCGGCGATGAATCTTGTTACACTGCCCGGGTATTGCGTGGCTGGAACCATAGGGCATAAATTGATGTCGGGAAAACCCACCAAAATTGATCTGGACAAGGACACACAAATTGATGTTCGATGCCAGATTCATCAGTTGTCTTTCAGTCCTCACACAGACGCCAAAGGAATTATGGATCTTGTAAAATTTCTCTCCCCTAAGCACGTCATACTTGTTCATGGCGAGAAGCCGAGGATGGCGATTCTAAAAGGAAAAATCCAGTCGGAGCTGGGAATTCAGTGTTACGACCCTGCAAACAATGAGACCGTGTCAGTCCCATCAACCCACTACGCGAAAGCAGTAGCTTCCGACACGTTCATCCGAAGCTGTTCGAATCCAAACTTCAAGTTCTCGAAAAGAAGTTCAGAAGATGAACATGGTTTGAGTTTGGGAACCAGAAATTCGACTCCAGGACTGCAAGTAAGCGATGAGAGGGCAGCAGAAGGGGTTTTGGTTATGGAGAAAAGCAAAAAAACCAAGGTAGTACACCAAGAGGAGCTTATGCTTATGTTAGGAGAGAAAAAGCACCAAGTACAATTTGCTTATTGCTGCCCTGTTCAGACCGGCAACTTGAAAGAGGCCAAAAGCACTTCTGGAAACGACGATCAGCTTTGCAAATCTGAGAGATGCTCTCGGCTTCTCCGCAGATTATCTGCGAAACTTTCAAATGAGTTTTCTGAGGGGAACATCCAAGACTTTGAGGATCACCTTGAAGTGGCATCGTTTCGCGCATCCGTTTGCTTGAAGGAAAACTGCCCTCACAGACTACTGGATGGCCATGGCGTTCGGAATAAATCTGAAGAAGCAGTTTATTTCTGCTGCAGTTGGGCAATGGCAGATGAGAAGCTTGCATGGCAAGTCATTTCAATCTGTCAAAACTTCTCCATGCATGAGCAAAGCAAGGGGGCTTCTACTCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000741 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0009553 GO:0009559 GO:0009561 GO:0009987 GO:0010197 GO:0016043 GO:0030154 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Beta-Casp Lactamase_B_6 RMMBL zf-RING_2

Protein Analysis

664

Amino Acids

74.45

Weight (kDa)

7.96

Isoelectric Point (pI)

37.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	15 - 189	5.4e-12	Metallo-beta-lactamase superfamily
Anti-Pycsar_Apyc1	PF23023	17 - 187	4.7e-08	Anti-Pycsar protein Apyc1
Lactamase_B_6	PF16661	22 - 194	1.6e-19	Metallo-beta-lactamase superfamily domain
Lactamase_B_2	PF12706	27 - 191	7e-06	Beta-lactamase superfamily domain
Beta-Casp	PF10996	247 - 365	9.6e-27	Beta-Casp domain
RMMBL	PF07521	381 - 439	2.3e-15	Zn-dependent metallo-hydrolase RNA specificity domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007353)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01730 AT2G01730
fragaria_vesca	FvH4_4g33520
malus_domestica	MD16G1018300.v1.1
prunus_persica	Prupe.1G333600_v2.0.a1
pyrus_communis	pycom16g01670
rosa_chinensis	RchiOBHm_Chr3g0480131 RchiOBHm_Chr4g0443031
rosa_laevigata	RLG00000005961
rosa_multiflora	Rmu_co8263599.1_g000001 Rmu_sc0006604.1_g000001 Rmu_sc0006604.1_g000004 Rmu_sc0008055.1_g000002 Rmu_sc0011261.1_g000006
rosa_roxburghii	Rroxscaffold_5G00383660
rosa_rugosa	Rorug04G0343900
rosa_samantha	Rh3AG232100 Rh3CG261900 Rh3DG257700 Rh4AG398100 Rh4BG409600 Rh4CG425100 Rh4DG404400
rosa_wichuraiana	Rw3G020840 Rw4G034290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	843
AasI	GACNNNNNNGTC	1 cut(s)	17
Acc36I	ACCTGC	2 cut(s)	424, 803
AccB1I	GGYRCC	1 cut(s)	26
AccBSI	CCGCTC	1 cut(s)	488
AccII	CGCG	3 cut(s)	639, 1364, 1800
AciI	CCGC	6 cut(s)	96, 124, 127, 258, 488, 1714
AclWI	GGATC	3 cut(s)	423, 1215, 1782
AcoI	YGGCCR	2 cut(s)	3, 1846
AcsI	RAATTY	5 cut(s)	592, 665, 1217, 1305, 1471
AcuI	CTGAAG	2 cut(s)	440, 1890
AcyI	GRCGYC	3 cut(s)	27, 516, 1191
AfaI	GTAC	4 cut(s)	530, 887, 1557, 1605
AfiI	CCNNNNNNNGG	1 cut(s)	1786
AflIII	ACRYGT	2 cut(s)	475, 1383
AjiI	CACGTC	1 cut(s)	1237
AjnI	CCWGG	3 cut(s)	952, 979, 1480
AleI	CACNNNNGTG	1 cut(s)	524
AloI	GAACNNNNNNTCC	2 cut(s)	948, 980
Alw21I	GWGCWC	1 cut(s)	742
Alw26I	GTCTC	5 cut(s)	309, 378, 507, 531, 1328
AlwI	GGATC	3 cut(s)	423, 1215, 1782
Ama87I	CYCGRG	2 cut(s)	654, 1058
AoxI	GGCC	3 cut(s)	3, 1650, 1846
ApeKI	GCWGC	6 cut(s)	51, 572, 695, 1508, 1620, 1890
ApoI	RAATTY	5 cut(s)	592, 665, 1217, 1305, 1471
ArsI	GACNNNNNNTTYG	2 cut(s)	287, 319
Asp700I	GAANNNNTTC	1 cut(s)	1399
AspLEI	GCGC	3 cut(s)	29, 780, 1802
AspS9I	GGNCC	4 cut(s)	269, 598, 861, 956
AsuC2I	CCSGG	5 cut(s)	31, 655, 656, 1059, 1060
AsuHPI	GGTGA	5 cut(s)	73, 196, 238, 365, 1769
AsuII	TTCGAA	1 cut(s)	1403
AvaI	CYCGRG	2 cut(s)	654, 1058
AvaII	GGWCC	4 cut(s)	269, 598, 861, 956
BalI	TGGCCA	2 cut(s)	5, 1848
BanI	GGYRCC	1 cut(s)	26
BanII	GRGCYC	3 cut(s)	235, 742, 1030
Bbv12I	GWGCWC	1 cut(s)	742
BbvI	GCAGC	6 cut(s)	38, 584, 682, 1520, 1607, 1877
BccI	CCATC	5 cut(s)	14, 331, 1261, 1357, 1838
BciT130I	CCWGG	3 cut(s)	954, 981, 1482
BciVI	GTATCC	1 cut(s)	297
BclI	TGATCA	1 cut(s)	987
BcnI	CCSGG	5 cut(s)	31, 655, 656, 1059, 1060
BcoDI	GTCTC	5 cut(s)	309, 378, 507, 531, 1328
BfmI	CTRYAG	2 cut(s)	1891, 1988
BfoI	RGCGCY	1 cut(s)	30
BfuAI	ACCTGC	2 cut(s)	424, 803
BfuI	GTATCC	1 cut(s)	297
BisI	GCNGC	8 cut(s)	52, 127, 489, 573, 696, 1509, 1621, 1891
BlsI	GCNGC	8 cut(s)	53, 128, 490, 574, 697, 1510, 1622, 1892
Bme1390I	CCNGG	8 cut(s)	31, 655, 656, 954, 981, 1059, 1060, 1482
Bme18I	GGWCC	4 cut(s)	269, 598, 861, 956
BmeT110I	CYCGRG	2 cut(s)	654, 1058
BmgBI	CACGTC	1 cut(s)	1237
BmgT120I	GGNCC	4 cut(s)	269, 598, 861, 956
BmiI	GGNNCC	7 cut(s)	28, 270, 795, 958, 1029, 1078, 1465
BmrFI	CCNGG	8 cut(s)	31, 655, 656, 954, 981, 1059, 1060, 1482
BmrI	ACTGGG	2 cut(s)	781, 1033
BmsI	GCATC	8 cut(s)	204, 571, 837, 933, 1142, 1688, 1799, 1811
BmuI	ACTGGG	2 cut(s)	781, 1033
BplI	GAGNNNNNCTC	6 cut(s)	654, 686, 1685, 1717, 1687, 1719
BpmI	CTGGAG	1 cut(s)	1464
Bpu10I	CCTNAGC	2 cut(s)	741, 1230
Bpu14I	TTCGAA	1 cut(s)	1403
BpuEI	CTTGAG	1 cut(s)	1028
BpuMI	CCSGG	5 cut(s)	31, 655, 656, 1059, 1060
BsaAI	YACGTR	1 cut(s)	621
BsaBI	GATNNNNATC	2 cut(s)	561, 851
BsaHI	GRCGYC	3 cut(s)	27, 516, 1191
BsaI	GGTCTC	1 cut(s)	1328
BsaJI	CCNNGG	8 cut(s)	22, 249, 654, 980, 1058, 1262, 1548, 1849
Bsc4I	CCNNNNNNNGG	1 cut(s)	1786
Bse118I	RCCGGY	2 cut(s)	698, 1634
Bse1I	ACTGG	5 cut(s)	776, 864, 1028, 1291, 1845
Bse3DI	GCAATG	3 cut(s)	381, 498, 1908
Bse8I	GATNNNNATC	2 cut(s)	561, 851
BseBI	CCWGG	3 cut(s)	954, 981, 1482
BseDI	CCNNGG	8 cut(s)	22, 249, 654, 980, 1058, 1262, 1548, 1849
BseGI	GGATG	6 cut(s)	990, 1272, 1389, 1758, 1802, 1849
BseJI	GATNNNNATC	2 cut(s)	561, 851
BseLI	CCNNNNNNNGG	1 cut(s)	1786
BseMI	GCAATG	3 cut(s)	381, 498, 1908
BseMII	CTCAG	5 cut(s)	685, 755, 822, 1683, 1740
BseNI	ACTGG	5 cut(s)	776, 864, 1028, 1291, 1845
BseRI	GAGGAG	1 cut(s)	1580
BseXI	GCAGC	6 cut(s)	38, 584, 682, 1520, 1607, 1877
BseYI	CCCAGC	1 cut(s)	1300
BsgI	GTGCAG	1 cut(s)	452
Bsh1236I	CGCG	3 cut(s)	639, 1364, 1800
Bsh1285I	CGRYCG	1 cut(s)	934
BshFI	GGCC	3 cut(s)	5, 1652, 1848
BshNI	GGYRCC	1 cut(s)	26
BsiEI	CGRYCG	1 cut(s)	934
BsiHKAI	GWGCWC	1 cut(s)	742
BsiHKCI	CYCGRG	2 cut(s)	654, 1058
BsiSI	CCGG	5 cut(s)	30, 655, 699, 1059, 1635
BslFI	GGGAC	1 cut(s)	1331
BslI	CCNNNNNNNGG	1 cut(s)	1786
BsmAI	GTCTC	5 cut(s)	309, 378, 507, 531, 1328
BsmBI	CGTCTC	2 cut(s)	378, 507
BsmFI	GGGAC	1 cut(s)	1331
BsmI	GAATGC	2 cut(s)	105, 147
BsnI	GGCC	3 cut(s)	5, 1652, 1848
Bso31I	GGTCTC	1 cut(s)	1328
BsoBI	CYCGRG	2 cut(s)	654, 1058
Bsp119I	TTCGAA	1 cut(s)	1403
Bsp1286I	GDGCHC	3 cut(s)	235, 742, 1030
Bsp1407I	TGTACA	1 cut(s)	528
Bsp143I	GATC	8 cut(s)	178, 220, 415, 987, 1120, 1207, 1675, 1774
Bsp19I	CCATGG	1 cut(s)	1849
BspACI	CCGC	6 cut(s)	96, 124, 127, 258, 488, 1714
BspANI	GGCC	3 cut(s)	5, 1652, 1848
BspCNI	CTCAG	5 cut(s)	684, 754, 821, 1684, 1741
BspFNI	CGCG	3 cut(s)	639, 1364, 1800
BspLI	GGNNCC	7 cut(s)	28, 270, 795, 958, 1029, 1078, 1465
BspMAI	CTGCAG	1 cut(s)	1895
BspMI	ACCTGC	2 cut(s)	424, 803
BspPI	GGATC	3 cut(s)	423, 1215, 1782
BspQI	GCTCTTC	3 cut(s)	240, 354, 730
BspT104I	TTCGAA	1 cut(s)	1403
BspT107I	GGYRCC	1 cut(s)	26
BspTNI	GGTCTC	1 cut(s)	1328
BsrBI	CCGCTC	1 cut(s)	488
BsrDI	GCAATG	3 cut(s)	381, 498, 1908
BsrFI	RCCGGY	2 cut(s)	698, 1634
BsrGI	TGTACA	1 cut(s)	528
BsrI	ACTGG	5 cut(s)	776, 864, 1028, 1291, 1845
BssAI	RCCGGY	2 cut(s)	698, 1634
BssECI	CCNNGG	8 cut(s)	22, 249, 654, 980, 1058, 1262, 1548, 1849
BssMI	GATC	8 cut(s)	178, 220, 415, 987, 1120, 1207, 1675, 1774
BssNI	GRCGYC	3 cut(s)	27, 516, 1191
BssT1I	CCWWGG	2 cut(s)	1548, 1849
Bst2UI	CCWGG	3 cut(s)	954, 981, 1482
Bst4CI	ACNGT	4 cut(s)	303, 430, 935, 1339
Bst6I	CTCTTC	3 cut(s)	240, 354, 730
BstACI	GRCGYC	3 cut(s)	27, 516, 1191
BstAPI	GCANNNNNTGC	2 cut(s)	430, 467
BstAUI	TGTACA	1 cut(s)	528
BstBAI	YACGTR	1 cut(s)	621
BstBI	TTCGAA	1 cut(s)	1403
BstC8I	GCNNGC	2 cut(s)	948, 1920
BstDEI	CTNAG	7 cut(s)	217, 671, 741, 808, 1230, 1692, 1749
BstDSI	CCRYGG	1 cut(s)	1849
BstF5I	GGATG	6 cut(s)	990, 1272, 1389, 1758, 1802, 1849
BstFNI	CGCG	3 cut(s)	639, 1364, 1800
BstH2I	RGCGCY	1 cut(s)	30
BstHHI	GCGC	3 cut(s)	29, 780, 1802
BstKTI	GATC	8 cut(s)	181, 223, 418, 990, 1123, 1210, 1678, 1777
BstMAI	GTCTC	5 cut(s)	309, 378, 507, 531, 1328
BstMBI	GATC	8 cut(s)	178, 220, 415, 987, 1120, 1207, 1675, 1774
BstMCI	CGRYCG	1 cut(s)	934
BstMWI	GCNNNNNNNGC	4 cut(s)	430, 467, 1034, 1924
BstNI	CCWGG	3 cut(s)	954, 981, 1482
BstNSI	RCATGY	2 cut(s)	479, 950
BstSCI	CCNGG	8 cut(s)	29, 653, 654, 952, 979, 1057, 1058, 1480
BstSFI	CTRYAG	2 cut(s)	1891, 1988
BstUI	CGCG	3 cut(s)	639, 1364, 1800
BstV1I	GCAGC	6 cut(s)	38, 584, 682, 1520, 1607, 1877
BstX2I	RGATCY	2 cut(s)	415, 1207
BstYI	RGATCY	2 cut(s)	415, 1207
BsuI	GTATCC	1 cut(s)	297
BsuRI	GGCC	3 cut(s)	5, 1652, 1848
BtgI	CCRYGG	1 cut(s)	1849
BtgZI	GCGATG	2 cut(s)	1052, 1512
BtrI	CACGTC	1 cut(s)	1237
BtsCI	GGATG	6 cut(s)	990, 1272, 1389, 1758, 1802, 1849
BtsI	GCAGTG	1 cut(s)	1052
BtsIMutI	CAGTG	6 cut(s)	299, 435, 997, 1021, 1052, 1316
BveI	ACCTGC	2 cut(s)	424, 803
Cac8I	GCNNGC	2 cut(s)	948, 1920
CfoI	GCGC	3 cut(s)	29, 780, 1802
Cfr10I	RCCGGY	2 cut(s)	698, 1634
Cfr13I	GGNCC	4 cut(s)	269, 598, 861, 956
Cfr9I	CCCGGG	2 cut(s)	654, 1058
CseI	GACGC	2 cut(s)	524, 1199
Csp6I	GTAC	4 cut(s)	529, 886, 1556, 1604
CspCI	CAANNNNNGTGG	2 cut(s)	1099, 1134
CviQI	GTAC	4 cut(s)	529, 886, 1556, 1604
DdeI	CTNAG	7 cut(s)	217, 671, 741, 808, 1230, 1692, 1749
DinI	GGCGCC	1 cut(s)	28
DpnI	GATC	8 cut(s)	180, 222, 417, 989, 1122, 1209, 1677, 1776
DpnII	GATC	8 cut(s)	178, 220, 415, 987, 1120, 1207, 1675, 1774
DrdI	GACNNNNNNGTC	1 cut(s)	17
DseDI	GACNNNNNNGTC	1 cut(s)	17
EaeI	YGGCCR	2 cut(s)	3, 1846
Eam1104I	CTCTTC	3 cut(s)	240, 354, 730
EarI	CTCTTC	3 cut(s)	240, 354, 730
Ecl136II	GAGCTC	1 cut(s)	740
Eco130I	CCWWGG	2 cut(s)	1548, 1849
Eco24I	GRGCYC	3 cut(s)	235, 742, 1030
Eco31I	GGTCTC	1 cut(s)	1328
Eco47I	GGWCC	4 cut(s)	269, 598, 861, 956
Eco53kI	GAGCTC	1 cut(s)	740
Eco57I	CTGAAG	2 cut(s)	440, 1890
Eco88I	CYCGRG	2 cut(s)	654, 1058
EcoICRI	GAGCTC	1 cut(s)	740
EcoRI	GAATTC	1 cut(s)	1305
EcoRII	CCWGG	3 cut(s)	952, 979, 1480
EcoT14I	CCWWGG	2 cut(s)	1548, 1849
EcoT22I	ATGCAT	2 cut(s)	948, 1962
EcoT38I	GRGCYC	3 cut(s)	235, 742, 1030
EgeI	GGCGCC	1 cut(s)	28
EheI	GGCGCC	1 cut(s)	28
ErhI	CCWWGG	2 cut(s)	1548, 1849
Esp3I	CGTCTC	2 cut(s)	378, 507
FalI	AAGNNNNNCTT	2 cut(s)	1554, 1586
FaqI	GGGAC	1 cut(s)	1331
FbaI	TGATCA	1 cut(s)	987
Fnu4HI	GCNGC	8 cut(s)	52, 127, 489, 573, 696, 1509, 1621, 1891
FokI	GGATG	6 cut(s)	997, 1279, 1376, 1745, 1789, 1856
FriOI	GRGCYC	3 cut(s)	235, 742, 1030
Fsp4HI	GCNGC	8 cut(s)	52, 127, 489, 573, 696, 1509, 1621, 1891
GlaI	GCGC	3 cut(s)	28, 779, 1801
GluI	GCNGC	8 cut(s)	52, 127, 489, 573, 696, 1509, 1621, 1891
GsaI	CCCAGC	1 cut(s)	1304
GsuI	CTGGAG	1 cut(s)	1464
HaeII	RGCGCY	1 cut(s)	30
HaeIII	GGCC	3 cut(s)	5, 1652, 1848
HapII	CCGG	5 cut(s)	30, 655, 699, 1059, 1635
HgaI	GACGC	2 cut(s)	524, 1199
HhaI	GCGC	3 cut(s)	29, 780, 1802
Hin1I	GRCGYC	3 cut(s)	27, 516, 1191
Hin6I	GCGC	3 cut(s)	27, 778, 1800
HinP1I	GCGC	3 cut(s)	27, 778, 1800
HindIII	AAGCTT	1 cut(s)	1916
HpaII	CCGG	5 cut(s)	30, 655, 699, 1059, 1635
HphI	GGTGA	5 cut(s)	73, 196, 238, 365, 1769
Hpy166II	GTNNAC	3 cut(s)	529, 531, 1558
Hpy188III	TCNNGA	6 cut(s)	116, 743, 1101, 1124, 1423, 1665
Hpy8I	GTNNAC	3 cut(s)	529, 531, 1558
Hpy99I	CGWCG	1 cut(s)	1676
HpyAV	CCTTC	7 cut(s)	342, 458, 611, 833, 1041, 1508, 1810
HpyCH4III	ACNGT	4 cut(s)	303, 430, 935, 1339
HpyCH4IV	ACGT	6 cut(s)	225, 284, 620, 884, 1236, 1385
HpyF10VI	GCNNNNNNNGC	4 cut(s)	430, 467, 1034, 1924
HpyF3I	CTNAG	7 cut(s)	217, 671, 741, 808, 1230, 1692, 1749
HpySE526I	ACGT	6 cut(s)	225, 284, 620, 884, 1236, 1385
Hsp92I	GRCGYC	3 cut(s)	27, 516, 1191
HspAI	GCGC	3 cut(s)	27, 778, 1800
KasI	GGCGCC	1 cut(s)	26
Ksp22I	TGATCA	1 cut(s)	987
Kzo9I	GATC	8 cut(s)	178, 220, 415, 987, 1120, 1207, 1675, 1774
LguI	GCTCTTC	3 cut(s)	240, 354, 730
LmnI	GCTCC	8 cut(s)	343, 485, 569, 775, 955, 1033, 1297, 1567
Lsp1109I	GCAGC	6 cut(s)	38, 584, 682, 1520, 1607, 1877
LweI	GCATC	8 cut(s)	204, 571, 837, 933, 1142, 1688, 1799, 1811
MaeII	ACGT	6 cut(s)	225, 284, 620, 884, 1236, 1385
MaeIII	GTNAC	3 cut(s)	61, 1048, 1313
MalI	GATC	8 cut(s)	180, 222, 417, 989, 1122, 1209, 1677, 1776
MbiI	CCGCTC	1 cut(s)	488
MboI	GATC	8 cut(s)	178, 220, 415, 987, 1120, 1207, 1675, 1774
MboII	GAAGA	7 cut(s)	227, 371, 409, 747, 776, 1451, 1883
MflI	RGATCY	2 cut(s)	415, 1207
MhlI	GDGCHC	3 cut(s)	235, 742, 1030
MlsI	TGGCCA	2 cut(s)	5, 1848
MluNI	TGGCCA	2 cut(s)	5, 1848
Mly113I	GGCGCC	1 cut(s)	27
MlyI	GAGTC	3 cut(s)	5, 176, 1471
MmeI	TCCRAC	7 cut(s)	297, 314, 741, 743, 839, 1275, 1404
Mox20I	TGGCCA	2 cut(s)	5, 1848
Mph1103I	ATGCAT	2 cut(s)	948, 1962
MroXI	GAANNNNTTC	1 cut(s)	1399
MscI	TGGCCA	2 cut(s)	5, 1848
MseI	TTAA	1 cut(s)	912
MslI	CAYNNNNRTG	2 cut(s)	524, 687
Msp20I	TGGCCA	2 cut(s)	5, 1848
MspI	CCGG	5 cut(s)	30, 655, 699, 1059, 1635
MspR9I	CCNGG	8 cut(s)	31, 655, 656, 954, 981, 1059, 1060, 1482
Mva1269I	GAATGC	2 cut(s)	105, 147
MvaI	CCWGG	3 cut(s)	954, 981, 1482
MvnI	CGCG	3 cut(s)	639, 1364, 1800
MwoI	GCNNNNNNNGC	4 cut(s)	430, 467, 1034, 1924
NarI	GGCGCC	1 cut(s)	27
NciI	CCSGG	5 cut(s)	31, 655, 656, 1059, 1060
NcoI	CCATGG	1 cut(s)	1849
NdeII	GATC	8 cut(s)	178, 220, 415, 987, 1120, 1207, 1675, 1774
NlaIV	GGNNCC	7 cut(s)	28, 270, 795, 958, 1029, 1078, 1465
NmeAIII	GCCGAG	3 cut(s)	412, 1287, 1684
NmuCI	GTSAC	1 cut(s)	61
NsiI	ATGCAT	2 cut(s)	948, 1962
NspI	RCATGY	2 cut(s)	479, 950
NspV	TTCGAA	1 cut(s)	1403
OliI	CACNNNNGTG	1 cut(s)	524
PaeI	GCATGC	1 cut(s)	950
PciI	ACATGT	1 cut(s)	475
PciSI	GCTCTTC	3 cut(s)	240, 354, 730
PcsI	WCGNNNNNNNCGW	1 cut(s)	15
PctI	GAATGC	2 cut(s)	105, 147
PdmI	GAANNNNTTC	1 cut(s)	1399
PfeI	GAWTC	8 cut(s)	81, 614, 641, 784, 1042, 1159, 1273, 1405
PflFI	GACNNNGTC	1 cut(s)	1339
PfoI	TCCNGGA	2 cut(s)	952, 1480
PkrI	GCNGC	8 cut(s)	53, 128, 490, 574, 697, 1510, 1622, 1892
PleI	GAGTC	3 cut(s)	5, 175, 1471
PluTI	GGCGCC	1 cut(s)	30
PpsI	GAGTC	3 cut(s)	5, 175, 1471
Ppu21I	YACGTR	1 cut(s)	621
PscI	ACATGT	1 cut(s)	475
PsiI	TTATAA	1 cut(s)	843
Psp124BI	GAGCTC	1 cut(s)	742
Psp6I	CCWGG	3 cut(s)	952, 979, 1480
PspFI	CCCAGC	1 cut(s)	1300
PspGI	CCWGG	3 cut(s)	952, 979, 1480
PspN4I	GGNNCC	7 cut(s)	28, 270, 795, 958, 1029, 1078, 1465
PspPI	GGNCC	4 cut(s)	269, 598, 861, 956
PstI	CTGCAG	1 cut(s)	1895
PsuI	RGATCY	2 cut(s)	415, 1207
PsyI	GACNNNGTC	1 cut(s)	1339
RsaI	GTAC	4 cut(s)	530, 887, 1557, 1605
RsaNI	GTAC	4 cut(s)	529, 886, 1556, 1604
RseI	CAYNNNNRTG	2 cut(s)	524, 687
SacI	GAGCTC	1 cut(s)	742
SapI	GCTCTTC	3 cut(s)	240, 354, 730
SaqAI	TTAA	1 cut(s)	912
SatI	GCNGC	8 cut(s)	52, 127, 489, 573, 696, 1509, 1621, 1891
Sau3AI	GATC	8 cut(s)	178, 220, 415, 987, 1120, 1207, 1675, 1774
Sau96I	GGNCC	4 cut(s)	269, 598, 861, 956
SchI	GAGTC	3 cut(s)	5, 176, 1471
ScrFI	CCNGG	8 cut(s)	31, 655, 656, 954, 981, 1059, 1060, 1482
SduI	GDGCHC	3 cut(s)	235, 742, 1030
SfaNI	GCATC	8 cut(s)	204, 571, 837, 933, 1142, 1688, 1799, 1811
SfcI	CTRYAG	2 cut(s)	1891, 1988
SfoI	GGCGCC	1 cut(s)	28
SfuI	TTCGAA	1 cut(s)	1403
SinI	GGWCC	4 cut(s)	269, 598, 861, 956
SmaI	CCCGGG	2 cut(s)	656, 1060
SmiMI	CAYNNNNRTG	2 cut(s)	524, 687
SmlI	CTYRAG	1 cut(s)	1007
SmoI	CTYRAG	1 cut(s)	1007
SphI	GCATGC	1 cut(s)	950
SsiI	CCGC	6 cut(s)	96, 124, 127, 258, 488, 1714
SspDI	GGCGCC	1 cut(s)	26
SstI	GAGCTC	1 cut(s)	742
StyD4I	CCNGG	8 cut(s)	29, 653, 654, 952, 979, 1057, 1058, 1480
StyI	CCWWGG	2 cut(s)	1548, 1849
TaaI	ACNGT	4 cut(s)	303, 430, 935, 1339
TaiI	ACGT	6 cut(s)	228, 287, 623, 887, 1239, 1388
TaqII	GACCGA	1 cut(s)	600
TatI	WGTACW	3 cut(s)	528, 1555, 1603
TauI	GCSGC	2 cut(s)	129, 491
TfiI	GAWTC	8 cut(s)	81, 614, 641, 784, 1042, 1159, 1273, 1405
Tru1I	TTAA	1 cut(s)	912
Tru9I	TTAA	1 cut(s)	912
TscAI	CASTG	6 cut(s)	306, 435, 997, 1028, 1059, 1316
TseFI	GTSAC	1 cut(s)	61
TseI	GCWGC	6 cut(s)	51, 572, 695, 1508, 1620, 1890
Tsp45I	GTSAC	1 cut(s)	61
TspDTI	ATGAA	8 cut(s)	410, 671, 797, 1055, 1151, 1238, 1378, 1458
TspGWI	ACGGA	2 cut(s)	446, 1795
TspMI	CCCGGG	2 cut(s)	654, 1058
TspRI	CASTG	6 cut(s)	306, 435, 997, 1028, 1059, 1316
Tth111I	GACNNNGTC	1 cut(s)	1339
VpaK11BI	GGWCC	4 cut(s)	269, 598, 861, 956
XapI	RAATTY	5 cut(s)	592, 665, 1217, 1305, 1471
XceI	RCATGY	2 cut(s)	479, 950
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1120, 1201
XmaI	CCCGGG	2 cut(s)	654, 1058
XmnI	GAANNNNTTC	1 cut(s)	1399
Zsp2I	ATGCAT	2 cut(s)	948, 1962

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.