Rorug04G0343900

Cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007353
Physical Location & Seq
Reverse (-)
51618136 .. 51620915
2780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0343900.1

Sequence Viewer

Length: 1542 bp
ATGGCTCTCAACACCCTACTACTAACCTTATGGCTTCCATTTCTCCTTCTCCCTCTACTGGTTTTACTGAAAAAGAATAAAAAGCCACAAAAACACTTCCCGCCAAGCCCTCCTAAGCTTCCCATCATAGGCAACTTGCACCAATTTGCACAATCTGGTTCATCACCTCACCAAAATCTATGGCGCCTCTCGAAAAAGTTCGGTCCAATCATGCTCTTACGCCTCGGTCGCATACCGACCCTCATAATCTCATCAGCTGAAGCAGCAAAACAGGTCTTGAAAGATAATGATCTCAACTGCTGCACCAGACCCTCCACCTCGGGGTCTCGAAAACTCACCTACAACTATATAGACATGGGATTTGCGCCTTATGGTGAGTATTGGCGAGAAATGAGAAAACTATGTGTGCTTGAGCTTTTCAGTGTGAAAAGGGTGCAGTCTTATCGGTTGATTAGGGAAGAAGAAGTGGCTAAAATGATTGACTCAATATCCAAAGAATCTGCATCTTGGTCCTCTAATTCATGTGTTAATCTTACTGAGAAGTTGTATGCTGTCATGGGCAGTATAACTTTCAGGGTTATTTTCGGGACAAGTTTCGAGGGAAGCGATTTCGAGCACGATAGGTTTCATCAAGTGCTTCATGAAGCTGAAATCATGCTTGCAGGCTTCTCTGCAGCTGATTATTTTCCTGCAATAATTGGGTATCTTATAGACAGGATCTCCGGGAAACATAAACAATTTGAAAGGGTATCCGGGGAACTTCATCACTTTTTTCAGCAGGTGATTGATGATCATCTCAAGCCAGGGAGGACACAACCAGAGCATGAAGACATAGTTGATGTGTTGCTTAAAATAGTGAAGGAGCAAACTGGTTTTGGAGCTGCTCAGTTCGGTCATGATAACATCAAGGCACTTCTTGTGAACTTATTTCTAGGTGGAATAGATACTGGTGCAATTACCATGCTGTGGGCGATGGCTGAGCTAGCTAAGAATCCTAGACTGATGAAGAAAGCACAGGATGAAGTTAGAAGCTGCGTTGGAAACAAAGGAAAAGTCTCTGAAAGTGATACGGAACAGCTTCCGTACCTCAAGATGATAATCAAAGAAACACTTCGACTGCATCCTCCAGCTCCCCTAATGCTTCCAAGAGAGTCCATGTCCCCCTTTAAAGTCCTTGGGTATGATGTTGAACCCAAAACACTAATCATAATTAATGACTGGGTAATTTCACGAGATCCAGAATTTTGGAAAGACCCAGAAGAGTTCATCCCAGAAAGGTTTGATGATAGCACTGTCGATTTTAGGGGGCAACACTTTGAGTACTTGCCATTTGGAGCTGGTCGGAGAATTTGTCCGGGAATTTACATGGCAACAACAACTGTGGAGCTTGGACTTGCAAATCTGCTGTACAGCTTTGATTGGAAATCGCCAGAGGGAATGAAAGAAGCTGATATCAACATGGAAGAAACAACTGGGGAACTTTCCCTAACTATCTCCAAGAAAACTGCTCTCAACCTTGTCCCCGTGAAGTTTTCTTGTTAG

Protein Analysis

513

Amino Acids

58.24

Weight (kDa)

7.23

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 34 - 493 2.4e-99 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 769
Acc36I ACCTGC 1 cut(s) 769
AccB1I GGYRCC 1 cut(s) 183
AccB7I CCANNNNNTGG 1 cut(s) 966
AciI CCGC 1 cut(s) 101
AclWI GGATC 2 cut(s) 725, 1229
AcsI RAATTY 3 cut(s) 1241, 1347, 1359
AcuI CTGAAG 1 cut(s) 279
AcyI GRCGYC 1 cut(s) 184
AfaI GTAC 3 cut(s) 1085, 1322, 1409
AfiI CCNNNNNNNGG 4 cut(s) 58, 128, 321, 966
AgsI TTSAA 3 cut(s) 280, 743, 1190
AjnI CCWGG 1 cut(s) 802
Alw21I GWGCWC 1 cut(s) 618
Alw26I GTCTC 2 cut(s) 330, 1060
AlwI GGATC 2 cut(s) 725, 1229
Ama87I CYCGRG 1 cut(s) 319
ApeKI GCWGC 5 cut(s) 263, 300, 674, 881, 1032
ApoI RAATTY 3 cut(s) 1241, 1347, 1359
ArsI GACNNNNNNTTYG 4 cut(s) 344, 376, 1038, 1070
AseI ATTAAT 1 cut(s) 1212
Asp700I GAANNNNTTC 3 cut(s) 197, 1077, 1110
AspLEI GCGC 2 cut(s) 186, 367
AspS9I GGNCC 2 cut(s) 203, 510
AsuC2I CCSGG 3 cut(s) 724, 754, 1356
AsuHPI GGTGA 5 cut(s) 156, 161, 328, 386, 793
AsuNHI GCTAGC 1 cut(s) 982
AvaI CYCGRG 1 cut(s) 319
AvaII GGWCC 2 cut(s) 203, 510
BanI GGYRCC 1 cut(s) 183
BauI CACGAG 1 cut(s) 1230
BbsI GAAGAC 1 cut(s) 834
Bbv12I GWGCWC 1 cut(s) 618
BbvI GCAGC 5 cut(s) 275, 287, 686, 868, 1019
BccI CCATC 2 cut(s) 131, 967
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciT130I CCWGG 1 cut(s) 804
BciVI GTATCC 1 cut(s) 760
BclI TGATCA 1 cut(s) 790
BcnI CCSGG 3 cut(s) 724, 754, 1356
BcoDI GTCTC 2 cut(s) 330, 1060
BfaI CTAG 3 cut(s) 932, 983, 996
BfmI CTRYAG 1 cut(s) 672
BfoI RGCGCY 1 cut(s) 187
BfuAI ACCTGC 1 cut(s) 769
BfuI GTATCC 1 cut(s) 760
BisI GCNGC 5 cut(s) 264, 301, 675, 882, 1033
BlpI GCTNAGC 1 cut(s) 978
BlsI GCNGC 5 cut(s) 265, 302, 676, 883, 1034
BmcAI AGTACT 1 cut(s) 1322
Bme1390I CCNGG 4 cut(s) 724, 754, 804, 1356
Bme18I GGWCC 2 cut(s) 203, 510
BmeT110I CYCGRG 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 203, 510
BmiI GGNNCC 1 cut(s) 185
BmrFI CCNGG 4 cut(s) 724, 754, 804, 1356
BmrI ACTGGG 2 cut(s) 1228, 1482
BmsI GCATC 2 cut(s) 512, 1129
BmtI GCTAGC 1 cut(s) 986
BmuI ACTGGG 2 cut(s) 1228, 1482
BpiI GAAGAC 1 cut(s) 834
BpmI CTGGAG 1 cut(s) 1110
Bpu10I CCTNAGC 1 cut(s) 114
Bpu1102I GCTNAGC 1 cut(s) 978
BpuEI CTTGAG 3 cut(s) 431, 782, 1073
BpuMI CCSGG 3 cut(s) 724, 754, 1356
BsaBI GATNNNNATC 3 cut(s) 288, 792, 1097
BsaHI GRCGYC 1 cut(s) 184
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 5 cut(s) 223, 318, 753, 803, 1174
BsaXI ACNNNNNCTCC 4 cut(s) 704, 734, 1336, 1366
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 128, 321, 966
Bse1I ACTGG 5 cut(s) 63, 874, 952, 1223, 1477
Bse8I GATNNNNATC 3 cut(s) 288, 792, 1097
BseBI CCWGG 1 cut(s) 804
BseDI CCNNGG 5 cut(s) 223, 318, 753, 803, 1174
BseGI GGATG 3 cut(s) 1024, 1120, 1266
BseJI GATNNNNATC 3 cut(s) 288, 792, 1097
BseLI CCNNNNNNNGG 4 cut(s) 58, 128, 321, 966
BseMII CTCAG 3 cut(s) 528, 899, 969
BseNI ACTGG 5 cut(s) 63, 874, 952, 1223, 1477
BseXI GCAGC 5 cut(s) 275, 287, 686, 868, 1019
BsgI GTGCAG 2 cut(s) 286, 455
Bsh1285I CGRYCG 1 cut(s) 229
BshNI GGYRCC 1 cut(s) 183
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 618
BsiHKCI CYCGRG 1 cut(s) 319
BsiSI CCGG 3 cut(s) 723, 753, 1355
BslFI GGGAC 3 cut(s) 601, 1144, 1505
BslI CCNNNNNNNGG 4 cut(s) 58, 128, 321, 966
BsmAI GTCTC 2 cut(s) 330, 1060
BsmFI GGGAC 3 cut(s) 601, 1144, 1505
Bso31I GGTCTC 1 cut(s) 330
BsoBI CYCGRG 1 cut(s) 319
Bsp1286I GDGCHC 1 cut(s) 618
Bsp1407I TGTACA 1 cut(s) 1407
Bsp143I GATC 4 cut(s) 289, 717, 790, 1234
Bsp1720I GCTNAGC 1 cut(s) 978
BspACI CCGC 1 cut(s) 101
BspCNI CTCAG 3 cut(s) 529, 898, 970
BspHI TCATGA 2 cut(s) 640, 895
BspLI GGNNCC 1 cut(s) 185
BspMAI CTGCAG 1 cut(s) 676
BspMI ACCTGC 1 cut(s) 769
BspOI GCTAGC 1 cut(s) 986
BspPI GGATC 2 cut(s) 725, 1229
BspT107I GGYRCC 1 cut(s) 183
BspTNI GGTCTC 1 cut(s) 330
BsrGI TGTACA 1 cut(s) 1407
BsrI ACTGG 5 cut(s) 63, 874, 952, 1223, 1477
BssECI CCNNGG 5 cut(s) 223, 318, 753, 803, 1174
BssMI GATC 4 cut(s) 289, 717, 790, 1234
BssNI GRCGYC 1 cut(s) 184
BssSI CACGAG 1 cut(s) 1230
BssT1I CCWWGG 1 cut(s) 1174
Bst2BI CACGAG 1 cut(s) 1230
Bst2UI CCWGG 1 cut(s) 804
Bst4CI ACNGT 2 cut(s) 1294, 1381
Bst6I CTCTTC 1 cut(s) 1254
BstACI GRCGYC 1 cut(s) 184
BstAUI TGTACA 1 cut(s) 1407
BstC8I GCNNGC 3 cut(s) 660, 664, 984
BstDEI CTNAG 5 cut(s) 114, 537, 885, 978, 987
BstF5I GGATG 3 cut(s) 1024, 1120, 1266
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 2 cut(s) 186, 367
BstKTI GATC 4 cut(s) 292, 720, 793, 1237
BstMAI GTCTC 2 cut(s) 330, 1060
BstMBI GATC 4 cut(s) 289, 717, 790, 1234
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 3 cut(s) 228, 263, 983
BstNI CCWGG 1 cut(s) 804
BstSCI CCNGG 4 cut(s) 722, 752, 802, 1354
BstSFI CTRYAG 1 cut(s) 672
BstV1I GCAGC 5 cut(s) 275, 287, 686, 868, 1019
BstV2I GAAGAC 1 cut(s) 834
BstX2I RGATCY 2 cut(s) 717, 1234
BstXI CCANNNNNNTGG 1 cut(s) 1245
BstYI RGATCY 2 cut(s) 717, 1234
BsuI GTATCC 1 cut(s) 760
BtgZI GCGATG 1 cut(s) 986
BtsCI GGATG 3 cut(s) 1024, 1120, 1266
BtsIMutI CAGTG 2 cut(s) 427, 1290
BveI ACCTGC 1 cut(s) 769
Cac8I GCNNGC 3 cut(s) 660, 664, 984
CciI TCATGA 2 cut(s) 640, 895
CfoI GCGC 2 cut(s) 186, 367
Cfr13I GGNCC 2 cut(s) 203, 510
Csp6I GTAC 3 cut(s) 1084, 1321, 1408
CspCI CAANNNNNGTGG 2 cut(s) 1362, 1397
CviQI GTAC 3 cut(s) 1084, 1321, 1408
DdeI CTNAG 5 cut(s) 114, 537, 885, 978, 987
DinI GGCGCC 1 cut(s) 185
DpnI GATC 4 cut(s) 291, 719, 792, 1236
DpnII GATC 4 cut(s) 289, 717, 790, 1234
DraI TTTAAA 1 cut(s) 1168
Eam1104I CTCTTC 1 cut(s) 1254
EarI CTCTTC 1 cut(s) 1254
Eco130I CCWWGG 1 cut(s) 1174
Eco31I GGTCTC 1 cut(s) 330
Eco32I GATATC 1 cut(s) 1453
Eco47I GGWCC 2 cut(s) 203, 510
Eco57I CTGAAG 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 802
EcoRV GATATC 1 cut(s) 1453
EcoT14I CCWWGG 1 cut(s) 1174
EgeI GGCGCC 1 cut(s) 185
EheI GGCGCC 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 1174
FalI AAGNNNNNCTT 2 cut(s) 1095, 1127
FaqI GGGAC 3 cut(s) 601, 1144, 1505
FauI CCCGC 1 cut(s) 108
FbaI TGATCA 1 cut(s) 790
Fnu4HI GCNGC 5 cut(s) 264, 301, 675, 882, 1033
FokI GGATG 3 cut(s) 1031, 1107, 1253
Fsp4HI GCNGC 5 cut(s) 264, 301, 675, 882, 1033
FspBI CTAG 3 cut(s) 932, 983, 996
GlaI GCGC 2 cut(s) 185, 366
GluI GCNGC 5 cut(s) 264, 301, 675, 882, 1033
GsuI CTGGAG 1 cut(s) 1110
HaeII RGCGCY 1 cut(s) 187
HapII CCGG 3 cut(s) 723, 753, 1355
HhaI GCGC 2 cut(s) 186, 367
Hin1I GRCGYC 1 cut(s) 184
Hin6I GCGC 2 cut(s) 184, 365
HinP1I GCGC 2 cut(s) 184, 365
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 4 cut(s) 482, 497, 991, 1151
HpaII CCGG 3 cut(s) 723, 753, 1355
HphI GGTGA 5 cut(s) 156, 161, 328, 386, 793
Hpy166II GTNNAC 1 cut(s) 922
Hpy188I TCNGA 2 cut(s) 1060, 1344
Hpy188III TCNNGA 9 cut(s) 190, 277, 327, 586, 641, 896, 1090, 1230, 1238
Hpy8I GTNNAC 1 cut(s) 922
HpyAV CCTTC 2 cut(s) 56, 853
HpyCH4III ACNGT 2 cut(s) 1294, 1381
HpyF10VI GCNNNNNNNGC 3 cut(s) 228, 263, 983
HpyF3I CTNAG 5 cut(s) 114, 537, 885, 978, 987
Hsp92I GRCGYC 1 cut(s) 184
HspAI GCGC 2 cut(s) 184, 365
KasI GGCGCC 1 cut(s) 183
Ksp22I TGATCA 1 cut(s) 790
Kzo9I GATC 4 cut(s) 289, 717, 790, 1234
LmnI GCTCC 5 cut(s) 862, 878, 1135, 1334, 1384
Lsp1109I GCAGC 5 cut(s) 275, 287, 686, 868, 1019
LweI GCATC 2 cut(s) 512, 1129
MaeI CTAG 3 cut(s) 932, 983, 996
MalI GATC 4 cut(s) 291, 719, 792, 1236
MboI GATC 4 cut(s) 289, 717, 790, 1234
MboII GAAGA 6 cut(s) 470, 473, 839, 1018, 1271, 1475
MflI RGATCY 2 cut(s) 717, 1234
MhlI GDGCHC 1 cut(s) 618
Mly113I GGCGCC 1 cut(s) 184
MlyI GAGTC 2 cut(s) 476, 1160
MmeI TCCRAC 2 cut(s) 1018, 1322
MroXI GAANNNNTTC 3 cut(s) 197, 1077, 1110
MseI TTAA 4 cut(s) 528, 849, 1167, 1212
MspA1I CMGCKG 2 cut(s) 257, 677
MspI CCGG 3 cut(s) 723, 753, 1355
MspR9I CCNGG 4 cut(s) 724, 754, 804, 1356
MvaI CCWGG 1 cut(s) 804
MwoI GCNNNNNNNGC 3 cut(s) 228, 263, 983
NarI GGCGCC 1 cut(s) 184
NciI CCSGG 3 cut(s) 724, 754, 1356
NdeII GATC 4 cut(s) 289, 717, 790, 1234
NheI GCTAGC 1 cut(s) 982
NlaIV GGNNCC 1 cut(s) 185
PagI TCATGA 2 cut(s) 640, 895
PaqCI CACCTGC 1 cut(s) 769
PcsI WCGNNNNNNNCGW 1 cut(s) 603
PdmI GAANNNNTTC 3 cut(s) 197, 1077, 1110
PfeI GAWTC 2 cut(s) 497, 991
PflMI CCANNNNNTGG 1 cut(s) 966
PfoI TCCNGGA 2 cut(s) 722, 1354
PkrI GCNGC 5 cut(s) 265, 302, 676, 883, 1034
PleI GAGTC 2 cut(s) 476, 1159
PluTI GGCGCC 1 cut(s) 187
PpsI GAGTC 2 cut(s) 476, 1159
PshBI ATTAAT 1 cut(s) 1212
Psp6I CCWGG 1 cut(s) 802
PspGI CCWGG 1 cut(s) 802
PspN4I GGNNCC 1 cut(s) 185
PspPI GGNCC 2 cut(s) 203, 510
PstI CTGCAG 1 cut(s) 676
PsuI RGATCY 2 cut(s) 717, 1234
PvuII CAGCTG 2 cut(s) 257, 677
RsaI GTAC 3 cut(s) 1085, 1322, 1409
RsaNI GTAC 3 cut(s) 1084, 1321, 1408
SaqAI TTAA 4 cut(s) 528, 849, 1167, 1212
SatI GCNGC 5 cut(s) 264, 301, 675, 882, 1033
Sau3AI GATC 4 cut(s) 289, 717, 790, 1234
Sau96I GGNCC 2 cut(s) 203, 510
ScaI AGTACT 1 cut(s) 1322
SchI GAGTC 2 cut(s) 476, 1160
ScrFI CCNGG 4 cut(s) 724, 754, 804, 1356
SduI GDGCHC 1 cut(s) 618
SfaNI GCATC 2 cut(s) 512, 1129
SfcI CTRYAG 1 cut(s) 672
SfoI GGCGCC 1 cut(s) 185
SinI GGWCC 2 cut(s) 203, 510
SmlI CTYRAG 3 cut(s) 410, 797, 1088
SmoI CTYRAG 3 cut(s) 410, 797, 1088
SsiI CCGC 1 cut(s) 101
SspDI GGCGCC 1 cut(s) 183
SspMI CTAG 3 cut(s) 932, 983, 996
StyD4I CCNGG 4 cut(s) 722, 752, 802, 1354
StyI CCWWGG 1 cut(s) 1174
TaaI ACNGT 2 cut(s) 1294, 1381
TaqI TCGA 6 cut(s) 191, 328, 597, 612, 1114, 1296
TaqII GACCGA 3 cut(s) 191, 215, 881
TatI WGTACW 2 cut(s) 1320, 1407
TfiI GAWTC 2 cut(s) 497, 991
Tru1I TTAA 4 cut(s) 528, 849, 1167, 1212
Tru9I TTAA 4 cut(s) 528, 849, 1167, 1212
TscAI CASTG 2 cut(s) 427, 1297
TseI GCWGC 5 cut(s) 263, 300, 674, 881, 1032
TspGWI ACGGA 2 cut(s) 1071, 1085
TspRI CASTG 2 cut(s) 427, 1297
Van91I CCANNNNNTGG 1 cut(s) 966
VpaK11BI GGWCC 2 cut(s) 203, 510
VspI ATTAAT 1 cut(s) 1212
XapI RAATTY 3 cut(s) 1241, 1347, 1359
XmnI GAANNNNTTC 3 cut(s) 197, 1077, 1110
XspI CTAG 3 cut(s) 932, 983, 996
ZrmI AGTACT 1 cut(s) 1322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.