Rh3CG261900

Cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0007353
Physical Location & Seq
Forward (+)
25591909 .. 25596527
4619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG261900.1

Sequence Viewer

Length: 1926 bp
ATGGCCATCGACTCTCTCGTTCTGGGCGCTGGGCAAGAGGTAGGCAAGAGCTGTGTGATTGTGACGATAAACGGGAAACGCATCATGTTCGACTGTGGTATGCATATGGGCCACCTCGACCACCGCCGCTACCCGGACTTCTCTCTCATCGGCCCAAACTTCAATCAAACCCTCACTTGCATCATCATTACTCACTTTCATCTGGATCACATTGGCGCGCTCCCTTACTTCACTGAAGTCTGCGGTTACAGTGGCCCCATTTACATGACTTATCCGACGAAGGCGCTGGCGCCTTTGATGTTGGAGGATTATAGGAAAGTGATGGTTGAGAGAAGGGGAGAGGAGGAACAGTTTACGTCGGAGCACATTGTCGAATGCATGAAGAAAGTTATACCGGTGGACCTGAAGCAGACGGTGCAGGTTGATAAGGATCTTCAAATCCGTGCTTACTATGCAGGACATGTTCTTGGAGCTGCAATGTTTTATGCAAAGGTGGGAAACGCTGCTATGGTGTACACAGGAGACTATAATATGACACCAGATAGGCATCTTGGAGCAGCTCAAATTGATCGACTAAGTTTGGACCTTGTTATTACAGAGTCCACATATGCAACAACCATTCGTGATTCAAAATATGCCCGGGAAAGAGAATTTCTCAGAGCTGTTCACACTTGTGTTGCGGGTGGGGGAAAAGTGCTAATTCCTTCGTTTGCTCTGGGAAGAGCTCAGGAACTCTGTATCTTATTGGAAGACTACTGGGAGCGCATGAATTTAAAAGTTCCTATTTACTTCTCAGCAGGTTTGACGCGTCAAGCCAATATGTATTATAAGATGCTCATCAGTTGGACCAGCCAGAAAATCAAAGAGACATACTCCACACATAATGCATTTGATTTTAAAAATGTTCACAAATTTGAACGGTCTATGATAGATGCTCCTGGACCTTGTGTTCTGTTTGCCACACCAGGGATGATCAGTGGTGGTTTTTCACTTGAGGTTTTTAAGCACTGGGCTCCTTCAGAAAAGAATCTTGTTACAATGCCAGGGTATTGCGTGGCTGGAACCATAGGACATAAATTAATGTCGGGTAAACCGACCAAAATTGATCTGGACAAGGACACCAGAATTGATGTGCGATGCCAGATTCATCAATTGACTTTCAGTCCTCATACAGATGCCAAAGGGATTATGGATCTGGTGAAGTTTCTTTCTCCCAAGCACGTGATTCTTGTTCATGGTGAGAAGCCTAGGATGGCTACTCTAAAAGGAAGAATTCAATCCGAATTGGGAATTCAATGCTACGACCCTGCAAACAATGAGACCGTGTCAGTTCCGTCCACCCACTATGCGACAGCAGTAGCTTCTGATGCATTTGTCCAAAGTTGCTCAAATCCAAACTTCAAGTTTTCTACAAGCGGCTCAGAACACGAACAAGGTTCAAGTTCGAGTAACAGAAAATCCATTCCACGACTGCAAGTAAGTGATGATAGGGCAGCCGAAGGGATCTTGGTTATGGAGAAAAACAAAAAAGCCAAGGTAGTGCACCAAGATGAGCTCCTGGTCATGTTAGAAGAAAAGAAGCACCAAGTCAAGTTTGCATATTGCTGTCCTGCTCACATTGGTAACTTGCAAGAGAATCAAGAGACTGAGAGCACCGGCCTGCCTCTGTCTTCAACAAACAATGATATGCTTCAGCTATTGTCTGCAAAACTCACAAATGAACTCTCCGAGGTGAACATTCAAGATTTCGGGGATCACCTTCAAGTGGAGTCGTTTCGCGTGTCCATTTGTTCGAAGGAGGATTGCCCTTATAGATTAAGTGACAACGGTGGTGAAGAGCACTATGAACCAATGTTCTTTTGCTGCAGTTGGGCAATGGAAGATGAGAAGCTTGCATGGAAAGTCATCTCAATGTGTCAAAAATAA

Protein Analysis

641

Amino Acids

72.01

Weight (kDa)

6.42

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 15 - 179 1.6e-09 Metallo-beta-lactamase superfamily
Anti-Pycsar_Apyc1 PF23023 17 - 182 6.8e-09 Anti-Pycsar protein Apyc1
Lactamase_B_6 PF16661 20 - 189 1.3e-20 Metallo-beta-lactamase superfamily domain
Lactamase_B_2 PF12706 27 - 197 6.4e-06 Beta-lactamase superfamily domain
Beta-Casp PF10996 242 - 360 2.9e-28 Beta-Casp domain
RMMBL PF07521 375 - 434 9.7e-16 Zn-dependent metallo-hydrolase RNA specificity domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 828
Acc36I ACCTGC 2 cut(s) 409, 788
AccB1I GGYRCC 1 cut(s) 289
AccII CGCG 3 cut(s) 218, 808, 1779
AciI CCGC 5 cut(s) 124, 127, 243, 680, 1416
AclWI GGATC 5 cut(s) 213, 438, 1200, 1511, 1761
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 5 cut(s) 650, 769, 911, 1272, 1290
AcuI CTGAAG 4 cut(s) 255, 425, 1002, 1676
AcvI CACGTG 1 cut(s) 1222
AcyI GRCGYC 1 cut(s) 290
AfaI GTAC 1 cut(s) 515
AfiI CCNNNNNNNGG 3 cut(s) 133, 966, 1765
AflIII ACRYGT 2 cut(s) 460, 806
AgeI ACCGGT 1 cut(s) 394
AhdI GACNNNNNGTC 1 cut(s) 1161
AjnI CCWGG 4 cut(s) 937, 964, 1042, 1557
AleI CACNNNNGTG 1 cut(s) 672
AloI GAACNNNNNNTCC 2 cut(s) 933, 965
AluBI AGCT 9 cut(s) 51, 473, 560, 662, 725, 1361, 1555, 1696, 1891
AluI AGCT 9 cut(s) 51, 473, 560, 662, 725, 1361, 1555, 1696, 1891
Alw21I GWGCWC 6 cut(s) 366, 727, 1545, 1557, 1655, 1842
Alw26I GTCTC 4 cut(s) 516, 860, 1313, 1637
Alw44I GTGCAC 1 cut(s) 1541
AlwI GGATC 5 cut(s) 213, 438, 1200, 1511, 1761
Ama87I CYCGRG 1 cut(s) 639
AoxI GGCC 5 cut(s) 3, 109, 151, 253, 1657
ApaLI GTGCAC 1 cut(s) 1541
ApeKI GCWGC 5 cut(s) 473, 503, 557, 1493, 1863
ApoI RAATTY 5 cut(s) 650, 769, 911, 1272, 1290
AseI ATTAAT 1 cut(s) 1079
AsiGI ACCGGT 1 cut(s) 394
AspA2I CCTAGG 1 cut(s) 1247
AspLEI GCGC 6 cut(s) 29, 218, 220, 286, 292, 765
AspS9I GGNCC 7 cut(s) 109, 152, 254, 400, 583, 846, 941
AsuC2I CCSGG 3 cut(s) 134, 640, 641
AsuHPI GGTGA 5 cut(s) 1210, 1250, 1744, 1748, 1844
AsuII TTCGAA 1 cut(s) 1793
AvaI CYCGRG 1 cut(s) 639
AvaII GGWCC 4 cut(s) 400, 583, 846, 941
AvrII CCTAGG 1 cut(s) 1247
BaeGI GKGCMC 1 cut(s) 1545
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 289
BanII GRGCYC 3 cut(s) 727, 1015, 1557
BbrPI CACGTG 1 cut(s) 1222
BbsI GAAGAC 2 cut(s) 756, 1662
Bbv12I GWGCWC 6 cut(s) 366, 727, 1545, 1557, 1655, 1842
BbvI GCAGC 5 cut(s) 460, 490, 569, 1505, 1850
BccI CCATC 3 cut(s) 14, 316, 1246
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 4 cut(s) 939, 966, 1044, 1559
BclI TGATCA 1 cut(s) 972
BcnI CCSGG 3 cut(s) 134, 640, 641
BcoDI GTCTC 4 cut(s) 516, 860, 1313, 1637
BfaI CTAG 1 cut(s) 1248
BfmI CTRYAG 1 cut(s) 1864
BfoI RGCGCY 3 cut(s) 30, 287, 293
BfuAI ACCTGC 2 cut(s) 409, 788
BisI GCNGC 7 cut(s) 127, 474, 504, 558, 1417, 1494, 1864
BlnI CCTAGG 1 cut(s) 1247
BlsI GCNGC 7 cut(s) 128, 475, 505, 559, 1418, 1495, 1865
Bme1390I CCNGG 7 cut(s) 134, 640, 641, 939, 966, 1044, 1559
Bme18I GGWCC 4 cut(s) 400, 583, 846, 941
BmeRI GACNNNNNGTC 1 cut(s) 1161
BmeT110I CYCGRG 1 cut(s) 639
BmgT120I GGNCC 7 cut(s) 109, 152, 254, 400, 583, 846, 941
BmiI GGNNCC 4 cut(s) 256, 291, 1014, 1063
BmrFI CCNGG 7 cut(s) 134, 640, 641, 939, 966, 1044, 1559
BmrI ACTGGG 2 cut(s) 766, 1018
BmsI GCATC 8 cut(s) 90, 189, 556, 822, 922, 1127, 1165, 1357
BmuI ACTGGG 2 cut(s) 766, 1018
BpiI GAAGAC 2 cut(s) 756, 1662
BplI GAGNNNNNCTC 4 cut(s) 639, 671, 857, 889
Bpu10I CCTNAGC 1 cut(s) 726
Bpu14I TTCGAA 1 cut(s) 1793
BpuEI CTTGAG 1 cut(s) 1013
BpuMI CCSGG 3 cut(s) 134, 640, 641
BsaAI YACGTR 1 cut(s) 1222
BsaBI GATNNNNATC 3 cut(s) 429, 546, 836
BsaHI GRCGYC 1 cut(s) 290
BsaI GGTCTC 1 cut(s) 1313
BsaJI CCNNGG 6 cut(s) 639, 965, 1043, 1247, 1533, 1728
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 966, 1765
Bse118I RCCGGY 2 cut(s) 394, 1655
Bse1I ACTGG 2 cut(s) 761, 1013
Bse3DI GCAATG 2 cut(s) 483, 1881
Bse8I GATNNNNATC 3 cut(s) 429, 546, 836
BseBI CCWGG 4 cut(s) 939, 966, 1044, 1559
BseDI CCNNGG 6 cut(s) 639, 965, 1043, 1247, 1533, 1728
BseGI GGATG 2 cut(s) 975, 1257
BseJI GATNNNNATC 3 cut(s) 429, 546, 836
BseLI CCNNNNNNNGG 3 cut(s) 133, 966, 1765
BseMI GCAATG 2 cut(s) 483, 1881
BseMII CTCAG 5 cut(s) 670, 740, 807, 1434, 1638
BseNI ACTGG 2 cut(s) 761, 1013
BsePI GCGCGC 1 cut(s) 216
BseRI GAGGAG 1 cut(s) 356
BseSI GKGCMC 1 cut(s) 1545
BseXI GCAGC 5 cut(s) 460, 490, 569, 1505, 1850
BseYI CCCAGC 1 cut(s) 29
BsgI GTGCAG 1 cut(s) 437
Bsh1236I CGCG 3 cut(s) 218, 808, 1779
BshFI GGCC 5 cut(s) 5, 111, 153, 255, 1659
BshNI GGYRCC 1 cut(s) 289
BshTI ACCGGT 1 cut(s) 394
BsiHKAI GWGCWC 6 cut(s) 366, 727, 1545, 1557, 1655, 1842
BsiHKCI CYCGRG 1 cut(s) 639
BsiSI CCGG 4 cut(s) 134, 395, 640, 1656
BslI CCNNNNNNNGG 3 cut(s) 133, 966, 1765
BsmAI GTCTC 4 cut(s) 516, 860, 1313, 1637
BsmI GAATGC 1 cut(s) 380
BsnI GGCC 5 cut(s) 5, 111, 153, 255, 1659
Bso31I GGTCTC 1 cut(s) 1313
BsoBI CYCGRG 1 cut(s) 639
Bsp119I TTCGAA 1 cut(s) 1793
Bsp1286I GDGCHC 7 cut(s) 366, 727, 1015, 1545, 1557, 1655, 1842
Bsp1407I TGTACA 1 cut(s) 513
Bsp143I GATC 8 cut(s) 205, 430, 568, 972, 1105, 1192, 1503, 1753
BspACI CCGC 5 cut(s) 124, 127, 243, 680, 1416
BspANI GGCC 5 cut(s) 5, 111, 153, 255, 1659
BspCNI CTCAG 5 cut(s) 669, 739, 806, 1433, 1639
BspFNI CGCG 3 cut(s) 218, 808, 1779
BspLI GGNNCC 4 cut(s) 256, 291, 1014, 1063
BspMAI CTGCAG 1 cut(s) 1868
BspMI ACCTGC 2 cut(s) 409, 788
BspPI GGATC 5 cut(s) 213, 438, 1200, 1511, 1761
BspQI GCTCTTC 2 cut(s) 715, 1830
BspT104I TTCGAA 1 cut(s) 1793
BspT107I GGYRCC 1 cut(s) 289
BspTNI GGTCTC 1 cut(s) 1313
BsrDI GCAATG 2 cut(s) 483, 1881
BsrFI RCCGGY 2 cut(s) 394, 1655
BsrGI TGTACA 1 cut(s) 513
BsrI ACTGG 2 cut(s) 761, 1013
BssAI RCCGGY 2 cut(s) 394, 1655
BssECI CCNNGG 6 cut(s) 639, 965, 1043, 1247, 1533, 1728
BssHII GCGCGC 1 cut(s) 216
BssMI GATC 8 cut(s) 205, 430, 568, 972, 1105, 1192, 1503, 1753
BssNI GRCGYC 1 cut(s) 290
BssT1I CCWWGG 2 cut(s) 1247, 1533
Bst2UI CCWGG 4 cut(s) 939, 966, 1044, 1559
Bst4CI ACNGT 7 cut(s) 95, 251, 351, 415, 921, 1324, 1829
Bst6I CTCTTC 2 cut(s) 715, 1830
BstACI GRCGYC 1 cut(s) 290
BstAPI GCANNNNNTGC 1 cut(s) 415
BstAUI TGTACA 1 cut(s) 513
BstBAI YACGTR 1 cut(s) 1222
BstBI TTCGAA 1 cut(s) 1793
BstC8I GCNNGC 4 cut(s) 218, 288, 1661, 1893
BstDEI CTNAG 6 cut(s) 575, 656, 726, 793, 1420, 1647
BstF5I GGATG 2 cut(s) 975, 1257
BstFNI CGCG 3 cut(s) 218, 808, 1779
BstH2I RGCGCY 3 cut(s) 30, 287, 293
BstHHI GCGC 6 cut(s) 29, 218, 220, 286, 292, 765
BstKTI GATC 8 cut(s) 208, 433, 571, 975, 1108, 1195, 1506, 1756
BstMAI GTCTC 4 cut(s) 516, 860, 1313, 1637
BstMBI GATC 8 cut(s) 205, 430, 568, 972, 1105, 1192, 1503, 1753
BstMWI GCNNNNNNNGC 3 cut(s) 415, 452, 1367
BstNI CCWGG 4 cut(s) 939, 966, 1044, 1559
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 7 cut(s) 132, 638, 639, 937, 964, 1042, 1557
BstSFI CTRYAG 1 cut(s) 1864
BstSLI GKGCMC 1 cut(s) 1545
BstUI CGCG 3 cut(s) 218, 808, 1779
BstV1I GCAGC 5 cut(s) 460, 490, 569, 1505, 1850
BstV2I GAAGAC 2 cut(s) 756, 1662
BstX2I RGATCY 3 cut(s) 430, 1192, 1503
BstYI RGATCY 3 cut(s) 430, 1192, 1503
BsuRI GGCC 5 cut(s) 5, 111, 153, 255, 1659
BtgZI GCGATG 1 cut(s) 1150
BtsCI GGATG 2 cut(s) 975, 1257
BtsIMutI CAGTG 4 cut(s) 231, 256, 982, 1006
BveI ACCTGC 2 cut(s) 409, 788
Cac8I GCNNGC 4 cut(s) 218, 288, 1661, 1893
CfoI GCGC 6 cut(s) 29, 218, 220, 286, 292, 765
Cfr10I RCCGGY 2 cut(s) 394, 1655
Cfr13I GGNCC 7 cut(s) 109, 152, 254, 400, 583, 846, 941
Cfr9I CCCGGG 1 cut(s) 639
CseI GACGC 2 cut(s) 797, 814
Csp6I GTAC 1 cut(s) 514
CspAI ACCGGT 1 cut(s) 394
CviAII CATG 8 cut(s) 85, 265, 379, 461, 766, 1235, 1564, 1896
CviQI GTAC 1 cut(s) 514
DdeI CTNAG 6 cut(s) 575, 656, 726, 793, 1420, 1647
DinI GGCGCC 1 cut(s) 291
DpnI GATC 8 cut(s) 207, 432, 570, 974, 1107, 1194, 1505, 1755
DpnII GATC 8 cut(s) 205, 430, 568, 972, 1105, 1192, 1503, 1753
DraI TTTAAA 2 cut(s) 774, 898
DriI GACNNNNNGTC 1 cut(s) 1161
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 715, 1830
Eam1105I GACNNNNNGTC 1 cut(s) 1161
EarI CTCTTC 2 cut(s) 715, 1830
Ecl136II GAGCTC 2 cut(s) 725, 1555
Eco130I CCWWGG 2 cut(s) 1247, 1533
Eco24I GRGCYC 3 cut(s) 727, 1015, 1557
Eco31I GGTCTC 1 cut(s) 1313
Eco47I GGWCC 4 cut(s) 400, 583, 846, 941
Eco53kI GAGCTC 2 cut(s) 725, 1555
Eco57I CTGAAG 4 cut(s) 255, 425, 1002, 1676
Eco72I CACGTG 1 cut(s) 1222
Eco88I CYCGRG 1 cut(s) 639
EcoICRI GAGCTC 2 cut(s) 725, 1555
EcoRI GAATTC 2 cut(s) 1272, 1290
EcoRII CCWGG 4 cut(s) 937, 964, 1042, 1557
EcoT14I CCWWGG 2 cut(s) 1247, 1533
EcoT22I ATGCAT 4 cut(s) 105, 380, 889, 1372
EcoT38I GRGCYC 3 cut(s) 727, 1015, 1557
EgeI GGCGCC 1 cut(s) 291
EheI GGCGCC 1 cut(s) 291
ErhI CCWWGG 2 cut(s) 1247, 1533
FaeI CATG 8 cut(s) 88, 268, 382, 464, 769, 1238, 1567, 1899
FatI CATG 8 cut(s) 84, 264, 378, 460, 765, 1234, 1563, 1895
FauI CCCGC 1 cut(s) 673
FauNDI CATATG 2 cut(s) 105, 607
FbaI TGATCA 1 cut(s) 972
Fnu4HI GCNGC 7 cut(s) 127, 474, 504, 558, 1417, 1494, 1864
FokI GGATG 2 cut(s) 982, 1264
FriOI GRGCYC 3 cut(s) 727, 1015, 1557
Fsp4HI GCNGC 7 cut(s) 127, 474, 504, 558, 1417, 1494, 1864
FspBI CTAG 1 cut(s) 1248
GlaI GCGC 6 cut(s) 28, 217, 219, 285, 291, 764
GluI GCNGC 7 cut(s) 127, 474, 504, 558, 1417, 1494, 1864
GsaI CCCAGC 1 cut(s) 33
HaeII RGCGCY 3 cut(s) 30, 287, 293
HaeIII GGCC 5 cut(s) 5, 111, 153, 255, 1659
HapII CCGG 4 cut(s) 134, 395, 640, 1656
HgaI GACGC 2 cut(s) 797, 814
HhaI GCGC 6 cut(s) 29, 218, 220, 286, 292, 765
Hin1I GRCGYC 1 cut(s) 290
Hin1II CATG 8 cut(s) 88, 268, 382, 464, 769, 1238, 1567, 1899
Hin6I GCGC 6 cut(s) 27, 216, 218, 284, 290, 763
HinP1I GCGC 6 cut(s) 27, 216, 218, 284, 290, 763
HindIII AAGCTT 1 cut(s) 1889
HinfI GANTC 8 cut(s) 11, 599, 626, 1027, 1144, 1225, 1636, 1769
HpaII CCGG 4 cut(s) 134, 395, 640, 1656
HphI GGTGA 5 cut(s) 1210, 1250, 1744, 1748, 1844
Hpy188I TCNGA 8 cut(s) 276, 361, 659, 1021, 1282, 1366, 1423, 1729
Hpy188III TCNNGA 6 cut(s) 203, 623, 728, 1109, 1640, 1742
Hpy99I CGWCG 2 cut(s) 280, 361
HpyAV CCTTC 7 cut(s) 274, 327, 714, 1026, 1493, 1769, 1789
HpyCH4III ACNGT 7 cut(s) 95, 251, 351, 415, 921, 1324, 1829
HpyCH4IV ACGT 2 cut(s) 356, 1221
HpyF10VI GCNNNNNNNGC 3 cut(s) 415, 452, 1367
HpyF3I CTNAG 6 cut(s) 575, 656, 726, 793, 1420, 1647
HpySE526I ACGT 2 cut(s) 356, 1221
Hsp92I GRCGYC 1 cut(s) 290
Hsp92II CATG 8 cut(s) 88, 268, 382, 464, 769, 1238, 1567, 1899
HspAI GCGC 6 cut(s) 27, 216, 218, 284, 290, 763
KasI GGCGCC 1 cut(s) 289
Ksp22I TGATCA 1 cut(s) 972
Kzo9I GATC 8 cut(s) 205, 430, 568, 972, 1105, 1192, 1503, 1753
LguI GCTCTTC 2 cut(s) 715, 1830
LmnI GCTCC 8 cut(s) 225, 361, 470, 554, 760, 940, 1018, 1560
Lsp1109I GCAGC 5 cut(s) 460, 490, 569, 1505, 1850
LweI GCATC 8 cut(s) 90, 189, 556, 822, 922, 1127, 1165, 1357
MaeI CTAG 1 cut(s) 1248
MaeII ACGT 2 cut(s) 356, 1221
MaeIII GTNAC 6 cut(s) 61, 245, 1033, 1448, 1622, 1820
MalI GATC 8 cut(s) 207, 432, 570, 974, 1107, 1194, 1505, 1755
MboI GATC 8 cut(s) 205, 430, 568, 972, 1105, 1192, 1503, 1753
MboII GAAGA 9 cut(s) 394, 425, 732, 761, 1281, 1583, 1662, 1847, 1892
MfeI CAATTG 1 cut(s) 1151
MflI RGATCY 3 cut(s) 430, 1192, 1503
MhlI GDGCHC 7 cut(s) 366, 727, 1015, 1545, 1557, 1655, 1842
MlsI TGGCCA 1 cut(s) 5
MluI ACGCGT 1 cut(s) 806
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 290
MlyI GAGTC 3 cut(s) 5, 608, 1778
MmeI TCCRAC 4 cut(s) 282, 299, 339, 824
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 4 cut(s) 105, 380, 889, 1372
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 773, 897, 1002, 1079, 1817
MslI CAYNNNNRTG 5 cut(s) 263, 672, 1173, 1548, 1910
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 134, 395, 640, 1656
MspR9I CCNGG 7 cut(s) 134, 640, 641, 939, 966, 1044, 1559
MunI CAATTG 1 cut(s) 1151
Mva1269I GAATGC 1 cut(s) 380
MvaI CCWGG 4 cut(s) 939, 966, 1044, 1559
MvnI CGCG 3 cut(s) 218, 808, 1779
MwoI GCNNNNNNNGC 3 cut(s) 415, 452, 1367
NarI GGCGCC 1 cut(s) 290
NciI CCSGG 3 cut(s) 134, 640, 641
NdeI CATATG 2 cut(s) 105, 607
NdeII GATC 8 cut(s) 205, 430, 568, 972, 1105, 1192, 1503, 1753
NlaIII CATG 8 cut(s) 88, 268, 382, 464, 769, 1238, 1567, 1899
NlaIV GGNNCC 4 cut(s) 256, 291, 1014, 1063
NmuCI GTSAC 2 cut(s) 61, 1820
NsiI ATGCAT 4 cut(s) 105, 380, 889, 1372
NspI RCATGY 1 cut(s) 464
NspV TTCGAA 1 cut(s) 1793
OliI CACNNNNGTG 1 cut(s) 672
PauI GCGCGC 1 cut(s) 216
PciI ACATGT 1 cut(s) 460
PciSI GCTCTTC 2 cut(s) 715, 1830
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 1091
PctI GAATGC 1 cut(s) 380
PfeI GAWTC 5 cut(s) 626, 1027, 1144, 1225, 1636
PflFI GACNNNGTC 1 cut(s) 1324
PfoI TCCNGGA 1 cut(s) 937
PinAI ACCGGT 1 cut(s) 394
PkrI GCNGC 7 cut(s) 128, 475, 505, 559, 1418, 1495, 1865
PleI GAGTC 3 cut(s) 5, 607, 1777
PluTI GGCGCC 1 cut(s) 293
PmaCI CACGTG 1 cut(s) 1222
PmlI CACGTG 1 cut(s) 1222
PpsI GAGTC 3 cut(s) 5, 607, 1777
Ppu21I YACGTR 1 cut(s) 1222
PscI ACATGT 1 cut(s) 460
PshBI ATTAAT 1 cut(s) 1079
PsiI TTATAA 1 cut(s) 828
Psp124BI GAGCTC 2 cut(s) 727, 1557
Psp6I CCWGG 4 cut(s) 937, 964, 1042, 1557
PspCI CACGTG 1 cut(s) 1222
PspFI CCCAGC 1 cut(s) 29
PspGI CCWGG 4 cut(s) 937, 964, 1042, 1557
PspN4I GGNNCC 4 cut(s) 256, 291, 1014, 1063
PspPI GGNCC 7 cut(s) 109, 152, 254, 400, 583, 846, 941
PstI CTGCAG 1 cut(s) 1868
PsuI RGATCY 3 cut(s) 430, 1192, 1503
PsyI GACNNNGTC 1 cut(s) 1324
PteI GCGCGC 1 cut(s) 216
RsaI GTAC 1 cut(s) 515
RsaNI GTAC 1 cut(s) 514
RseI CAYNNNNRTG 5 cut(s) 263, 672, 1173, 1548, 1910
SacI GAGCTC 2 cut(s) 727, 1557
SapI GCTCTTC 2 cut(s) 715, 1830
SaqAI TTAA 5 cut(s) 773, 897, 1002, 1079, 1817
SatI GCNGC 7 cut(s) 127, 474, 504, 558, 1417, 1494, 1864
Sau3AI GATC 8 cut(s) 205, 430, 568, 972, 1105, 1192, 1503, 1753
Sau96I GGNCC 7 cut(s) 109, 152, 254, 400, 583, 846, 941
SchI GAGTC 3 cut(s) 5, 608, 1778
ScrFI CCNGG 7 cut(s) 134, 640, 641, 939, 966, 1044, 1559
SduI GDGCHC 7 cut(s) 366, 727, 1015, 1545, 1557, 1655, 1842
SfaNI GCATC 8 cut(s) 90, 189, 556, 822, 922, 1127, 1165, 1357
SfcI CTRYAG 1 cut(s) 1864
SfoI GGCGCC 1 cut(s) 291
SfuI TTCGAA 1 cut(s) 1793
SinI GGWCC 4 cut(s) 400, 583, 846, 941
SmaI CCCGGG 1 cut(s) 641
SmiMI CAYNNNNRTG 5 cut(s) 263, 672, 1173, 1548, 1910
SmlI CTYRAG 1 cut(s) 992
SmoI CTYRAG 1 cut(s) 992
SsiI CCGC 5 cut(s) 124, 127, 243, 680, 1416
SspDI GGCGCC 1 cut(s) 289
SspMI CTAG 1 cut(s) 1248
SstI GAGCTC 2 cut(s) 727, 1557
StyD4I CCNGG 7 cut(s) 132, 638, 639, 937, 964, 1042, 1557
StyI CCWWGG 2 cut(s) 1247, 1533
TaaI ACNGT 7 cut(s) 95, 251, 351, 415, 921, 1324, 1829
TaiI ACGT 2 cut(s) 359, 1224
TaqI TCGA 7 cut(s) 9, 90, 117, 372, 571, 1445, 1793
TatI WGTACW 1 cut(s) 513
TauI GCSGC 2 cut(s) 129, 1419
TfiI GAWTC 5 cut(s) 626, 1027, 1144, 1225, 1636
Tru1I TTAA 5 cut(s) 773, 897, 1002, 1079, 1817
Tru9I TTAA 5 cut(s) 773, 897, 1002, 1079, 1817
TscAI CASTG 4 cut(s) 238, 256, 982, 1013
TseFI GTSAC 2 cut(s) 61, 1820
TseI GCWGC 5 cut(s) 473, 503, 557, 1493, 1863
Tsp45I GTSAC 2 cut(s) 61, 1820
TspDTI ATGAA 7 cut(s) 188, 395, 782, 1136, 1223, 1734, 1860
TspGWI ACGGA 2 cut(s) 431, 1323
TspMI CCCGGG 1 cut(s) 639
TspRI CASTG 4 cut(s) 238, 256, 982, 1013
Tth111I GACNNNGTC 1 cut(s) 1324
VneI GTGCAC 1 cut(s) 1541
VpaK11BI GGWCC 4 cut(s) 400, 583, 846, 941
VspI ATTAAT 1 cut(s) 1079
XapI RAATTY 5 cut(s) 650, 769, 911, 1272, 1290
XceI RCATGY 1 cut(s) 464
XcmI CCANNNNNNNNNTGG 2 cut(s) 1105, 1186
XmaI CCCGGG 1 cut(s) 639
XmaJI CCTAGG 1 cut(s) 1247
XspI CTAG 1 cut(s) 1248
Zsp2I ATGCAT 4 cut(s) 105, 380, 889, 1372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.