FvH4_5g05320
NAC Family

inactive poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007192
Physical Location & Seq
Reverse (-)
3109880 .. 3113042
3163 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05320.t1

Sequence Viewer

Length: 1170 bp
ATGGAATACAGCGGTGTTCATCCTCCTTTATCCTCTGTTTTCTCCGATTTCACATCCCCGCCGCCTCAAGATTCCGATTGCGAGCTCTCCGGTCACGTTTCCGATTCCGGCACCACCGAGACGTCCTCCGGGAGCTCTGAGTATCAAGAATCGTCTGTCTCCGATTGCGAGAGCAGCGTCACAAGCGGCCGCGGCGGCGGTGACGTCAGCCCTGCGTTGGAGGCGGGGTTGGTGAAACTTGGAGAAGGGGACAGATTTTATGACCTGATCAAGAAAAGGTTTCTTGCGAGTTTGGGGGGATTGCTTGGACCGCAAGTAACGGTTTCGGCGATTAATAGAAACTCCCATTCGGGTTTCACGGGTCAAGCCCGGCTCAACGCGTTTCGGGTCTACTTGAAAGCCGTGGAGGACAAGTGTGGCGGCGACGCCAATGTGAAGTATGGTTGGTATGCGTCCAGTTCGAAAGAGGAGATTTCGAAGATTGTTAGCCATGGATTTGGTCAGTGTGAGAAGATTAATGGGGTTTATGGTAGAGGTGTCTATCTTGCTCCTGATGATTCTCCTTTGGAATGTTTGGAAAGCTTGAGTGCTGATGAAGACGGTATGCGGCATCTTTTGCTTTGCCGTGTGATTTTGGGGAAGCCGGAGGTTGTGCTGCCTGGTAATGCTGAACAGTATCATCCCAGTTCGTCGGAGTTTGATTCGGGTGTGGATAGGCTTTCCGCTCCCAAGAAGTATATTGTGTGGAGTACTCACATGAACACTCATATCTTGCCTGAGTATGTGATCAGCTTCAGAGCTCCTACTTGCTTGAAAGAGTTCTTGAAGACTCAGGAGCCGATTCGTAAACCAACTTCACCTTGGATGCCAATTCCAACCCTGATCGGTGTACTTTCAAAGGTCTTGCCTCAGCCTAGCATTGCTTTGATCATCAAGTATCACAAGGCCCACAGAGAAAATAAGATCTCAAGGAGTGAACTAATACAAAGGATTAGACAAATAGCAGGCGACAAGCTGCTGGCTACAATCATCAAGTCTTTCAGAACCAAGCAACTCAAACATTTGCACGGGGCTTCTCAAGACGGAGGCAGGAAGGCGATGGAGTACAAAAGTAGTGCGGGTGGTGTAGACGGACCTATGCTTTTTGGGGTGAACTTAAAACAGTGCTAA

Protein Analysis

390

Amino Acids

42.31

Weight (kDa)

7.62

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 283 - 348 7.3e-24 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 125, 207
AccB1I GGYRCC 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 725
AccI GTMKAC 2 cut(s) 390, 1128
AccII CGCG 2 cut(s) 192, 380
AcoI YGGCCR 1 cut(s) 187
AcuI CTGAAG 1 cut(s) 778
AcyI GRCGYC 3 cut(s) 122, 204, 426
AfaI GTAC 3 cut(s) 751, 891, 1106
AfiI CCNNNNNNNGG 1 cut(s) 217
AflIII ACRYGT 1 cut(s) 378
AgsI TTSAA 4 cut(s) 397, 814, 826, 897
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 6 cut(s) 85, 135, 582, 792, 800, 1015
AluI AGCT 6 cut(s) 85, 135, 582, 792, 800, 1015
Alw21I GWGCWC 3 cut(s) 87, 137, 802
Alw26I GTCTC 2 cut(s) 113, 163
AoxI GGCC 2 cut(s) 187, 945
ApeKI GCWGC 3 cut(s) 174, 655, 1015
AseI ATTAAT 2 cut(s) 333, 516
Asp700I GAANNNNTTC 1 cut(s) 818
AspS9I GGNCC 3 cut(s) 308, 946, 1133
AsuC2I CCSGG 2 cut(s) 130, 370
AsuHPI GGTGA 4 cut(s) 212, 244, 849, 1162
AsuII TTCGAA 2 cut(s) 461, 476
AvaII GGWCC 2 cut(s) 308, 1133
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 3 cut(s) 87, 137, 802
BarI GAAGNNNNNNTAC 2 cut(s) 838, 870
BbsI GAAGAC 2 cut(s) 603, 833
Bbv12I GWGCWC 3 cut(s) 87, 137, 802
BbvCI CCTCAGC 1 cut(s) 909
BbvI GCAGC 3 cut(s) 186, 642, 1002
BccI CCATC 1 cut(s) 1093
BceAI ACGGC 2 cut(s) 386, 609
BciT130I CCWGG 1 cut(s) 660
BclI TGATCA 3 cut(s) 267, 786, 927
BcnI CCSGG 2 cut(s) 130, 370
BcoDI GTCTC 2 cut(s) 113, 163
BfaI CTAG 1 cut(s) 915
BglI GCCNNNNNGGC 1 cut(s) 195
BglII AGATCT 1 cut(s) 963
BmcAI AGTACT 1 cut(s) 751
Bme1390I CCNGG 3 cut(s) 130, 370, 660
Bme18I GGWCC 2 cut(s) 308, 1133
BmgT120I GGNCC 3 cut(s) 308, 946, 1133
BmiI GGNNCC 2 cut(s) 112, 837
BmrFI CCNGG 3 cut(s) 130, 370, 660
BmrI ACTGGG 1 cut(s) 678
BmsI GCATC 2 cut(s) 619, 855
BmuI ACTGGG 1 cut(s) 678
BpiI GAAGAC 2 cut(s) 603, 833
BplI GAGNNNNNCTC 2 cut(s) 110, 142
Bpu10I CCTNAGC 1 cut(s) 909
Bpu14I TTCGAA 2 cut(s) 461, 476
BpuEI CTTGAG 4 cut(s) 51, 604, 952, 1062
BpuMI CCSGG 2 cut(s) 130, 370
BsaHI GRCGYC 3 cut(s) 122, 204, 426
BsaJI CCNNGG 4 cut(s) 190, 402, 490, 860
BsaWI WCCGGW 1 cut(s) 89
BsaXI ACNNNNNCTCC 4 cut(s) 212, 242, 398, 428
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse1I ACTGG 2 cut(s) 456, 684
Bse3DI GCAATG 1 cut(s) 918
BseBI CCWGG 1 cut(s) 660
BseDI CCNNGG 4 cut(s) 190, 402, 490, 860
BseGI GGATG 4 cut(s) 19, 53, 679, 870
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 918
BseMII CTCAG 4 cut(s) 129, 768, 845, 923
BseNI ACTGG 2 cut(s) 456, 684
BseRI GAGGAG 1 cut(s) 482
BseX3I CGGCCG 1 cut(s) 187
BseXI GCAGC 3 cut(s) 186, 642, 1002
Bsh1236I CGCG 2 cut(s) 192, 380
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 2 cut(s) 189, 947
BshNI GGYRCC 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 3 cut(s) 87, 137, 802
BsiSI CCGG 5 cut(s) 90, 108, 129, 370, 644
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 2 cut(s) 113, 163
BsmBI CGTCTC 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 2 cut(s) 189, 947
Bsp119I TTCGAA 2 cut(s) 461, 476
Bsp1286I GDGCHC 3 cut(s) 87, 137, 802
Bsp143I GATC 5 cut(s) 267, 786, 882, 927, 963
Bsp19I CCATGG 1 cut(s) 490
BspANI GGCC 2 cut(s) 189, 947
BspCNI CTCAG 4 cut(s) 130, 769, 844, 922
BspFNI CGCG 2 cut(s) 192, 380
BspLI GGNNCC 2 cut(s) 112, 837
BspT104I TTCGAA 2 cut(s) 461, 476
BspT107I GGYRCC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 725
BsrDI GCAATG 1 cut(s) 918
BsrI ACTGG 2 cut(s) 456, 684
BssECI CCNNGG 4 cut(s) 190, 402, 490, 860
BssMI GATC 5 cut(s) 267, 786, 882, 927, 963
BssNI GRCGYC 3 cut(s) 122, 204, 426
BssT1I CCWWGG 2 cut(s) 490, 860
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 4 cut(s) 322, 602, 675, 1164
BstACI GRCGYC 3 cut(s) 122, 204, 426
BstAPI GCANNNNNTGC 1 cut(s) 616
BstBI TTCGAA 2 cut(s) 461, 476
BstC8I GCNNGC 3 cut(s) 83, 1006, 1020
BstDEI CTNAG 4 cut(s) 138, 777, 831, 909
BstDSI CCRYGG 3 cut(s) 190, 402, 490
BstF5I GGATG 4 cut(s) 19, 53, 679, 870
BstFNI CGCG 2 cut(s) 192, 380
BstKTI GATC 5 cut(s) 270, 789, 885, 930, 966
BstMAI GTCTC 2 cut(s) 113, 163
BstMBI GATC 5 cut(s) 267, 786, 882, 927, 963
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 7 cut(s) 174, 183, 192, 195, 221, 310, 616
BstNI CCWGG 1 cut(s) 660
BstSCI CCNGG 3 cut(s) 128, 368, 658
BstUI CGCG 2 cut(s) 192, 380
BstV1I GCAGC 3 cut(s) 186, 642, 1002
BstV2I GAAGAC 2 cut(s) 603, 833
BstX2I RGATCY 1 cut(s) 963
BstXI CCANNNNNNTGG 1 cut(s) 497
BstYI RGATCY 1 cut(s) 963
BstZI CGGCCG 1 cut(s) 187
BsuRI GGCC 2 cut(s) 189, 947
BtgI CCRYGG 3 cut(s) 190, 402, 490
BtgZI GCGATG 1 cut(s) 1112
BtsCI GGATG 4 cut(s) 19, 53, 679, 870
BtsIMutI CAGTG 2 cut(s) 509, 1169
Cac8I GCNNGC 3 cut(s) 83, 1006, 1020
CciNI GCGGCCGC 1 cut(s) 187
Cfr13I GGNCC 3 cut(s) 308, 946, 1133
Cfr42I CCGCGG 1 cut(s) 193
CseI GACGC 3 cut(s) 166, 434, 441
Csp6I GTAC 3 cut(s) 750, 890, 1105
CviAII CATG 2 cut(s) 491, 757
CviQI GTAC 3 cut(s) 750, 890, 1105
DdeI CTNAG 4 cut(s) 138, 777, 831, 909
DpnI GATC 5 cut(s) 269, 788, 884, 929, 965
DpnII GATC 5 cut(s) 267, 786, 882, 927, 963
EaeI YGGCCR 1 cut(s) 187
EagI CGGCCG 1 cut(s) 187
Ecl136II GAGCTC 3 cut(s) 85, 135, 800
EclXI CGGCCG 1 cut(s) 187
Eco130I CCWWGG 2 cut(s) 490, 860
Eco24I GRGCYC 3 cut(s) 87, 137, 802
Eco47I GGWCC 2 cut(s) 308, 1133
Eco52I CGGCCG 1 cut(s) 187
Eco53kI GAGCTC 3 cut(s) 85, 135, 800
Eco57I CTGAAG 1 cut(s) 778
EcoICRI GAGCTC 3 cut(s) 85, 135, 800
EcoRII CCWGG 1 cut(s) 658
EcoT14I CCWWGG 2 cut(s) 490, 860
EcoT38I GRGCYC 3 cut(s) 87, 137, 802
ErhI CCWWGG 2 cut(s) 490, 860
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 2 cut(s) 494, 760
FaqI GGGAC 1 cut(s) 263
FatI CATG 2 cut(s) 490, 756
FauI CCCGC 3 cut(s) 66, 217, 1111
FbaI TGATCA 3 cut(s) 267, 786, 927
FblI GTMKAC 2 cut(s) 390, 1128
FokI GGATG 4 cut(s) 6, 40, 666, 877
FriOI GRGCYC 3 cut(s) 87, 137, 802
FspBI CTAG 1 cut(s) 915
HaeIII GGCC 2 cut(s) 189, 947
HapII CCGG 5 cut(s) 90, 108, 129, 370, 644
HgaI GACGC 3 cut(s) 166, 434, 441
Hin1I GRCGYC 3 cut(s) 122, 204, 426
Hin1II CATG 2 cut(s) 494, 760
HindIII AAGCTT 1 cut(s) 580
HinfI GANTC 7 cut(s) 71, 104, 149, 557, 701, 829, 841
HpaII CCGG 5 cut(s) 90, 108, 129, 370, 644
HphI GGTGA 4 cut(s) 212, 244, 849, 1162
Hpy166II GTNNAC 6 cut(s) 391, 848, 890, 977, 1129, 1153
Hpy188I TCNGA 8 cut(s) 46, 76, 103, 139, 163, 694, 797, 1043
Hpy188III TCNNGA 7 cut(s) 68, 146, 271, 551, 823, 833, 1079
Hpy8I GTNNAC 6 cut(s) 391, 848, 890, 977, 1129, 1153
Hpy99I CGWCG 2 cut(s) 428, 694
HpyAV CCTTC 2 cut(s) 239, 1087
HpyCH4III ACNGT 4 cut(s) 322, 602, 675, 1164
HpyCH4IV ACGT 3 cut(s) 96, 122, 204
HpyCH4V TGCA 1 cut(s) 1066
HpyF10VI GCNNNNNNNGC 7 cut(s) 174, 183, 192, 195, 221, 310, 616
HpyF3I CTNAG 4 cut(s) 138, 777, 831, 909
HpySE526I ACGT 3 cut(s) 96, 122, 204
Hsp92I GRCGYC 3 cut(s) 122, 204, 426
Hsp92II CATG 2 cut(s) 494, 760
Ksp22I TGATCA 3 cut(s) 267, 786, 927
KspI CCGCGG 1 cut(s) 193
Kzo9I GATC 5 cut(s) 267, 786, 882, 927, 963
LmnI GCTCC 5 cut(s) 132, 553, 730, 805, 835
Lsp1109I GCAGC 3 cut(s) 186, 642, 1002
LweI GCATC 2 cut(s) 619, 855
MaeI CTAG 1 cut(s) 915
MaeII ACGT 3 cut(s) 96, 122, 204
MaeIII GTNAC 4 cut(s) 92, 178, 200, 316
MalI GATC 5 cut(s) 269, 788, 884, 929, 965
MbiI CCGCTC 1 cut(s) 725
MboI GATC 5 cut(s) 267, 786, 882, 927, 963
MboII GAAGA 4 cut(s) 490, 523, 608, 838
MflI RGATCY 1 cut(s) 963
MhlI GDGCHC 3 cut(s) 87, 137, 802
MluCI AATT 1 cut(s) 870
MluI ACGCGT 1 cut(s) 378
MlyI GAGTC 1 cut(s) 823
MmeI TCCRAC 3 cut(s) 198, 672, 899
MroXI GAANNNNTTC 1 cut(s) 818
MseI TTAA 3 cut(s) 333, 516, 1157
MspA1I CMGCKG 2 cut(s) 12, 192
MspI CCGG 5 cut(s) 90, 108, 129, 370, 644
MspR9I CCNGG 3 cut(s) 130, 370, 660
MvaI CCWGG 1 cut(s) 660
MvnI CGCG 2 cut(s) 192, 380
MwoI GCNNNNNNNGC 7 cut(s) 174, 183, 192, 195, 221, 310, 616
NciI CCSGG 2 cut(s) 130, 370
NcoI CCATGG 1 cut(s) 490
NdeII GATC 5 cut(s) 267, 786, 882, 927, 963
NlaIII CATG 2 cut(s) 494, 760
NlaIV GGNNCC 2 cut(s) 112, 837
NmuCI GTSAC 3 cut(s) 92, 178, 200
NotI GCGGCCGC 1 cut(s) 187
NspV TTCGAA 2 cut(s) 461, 476
PcsI WCGNNNNNNNCGW 1 cut(s) 326
PdmI GAANNNNTTC 1 cut(s) 818
PfeI GAWTC 6 cut(s) 71, 104, 149, 557, 701, 841
PfoI TCCNGGA 1 cut(s) 128
PleI GAGTC 1 cut(s) 823
PpsI GAGTC 1 cut(s) 823
PshBI ATTAAT 2 cut(s) 333, 516
Psp124BI GAGCTC 3 cut(s) 87, 137, 802
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 2 cut(s) 112, 837
PspPI GGNCC 3 cut(s) 308, 946, 1133
PsuI RGATCY 1 cut(s) 963
RsaI GTAC 3 cut(s) 751, 891, 1106
RsaNI GTAC 3 cut(s) 750, 890, 1105
SacI GAGCTC 3 cut(s) 87, 137, 802
SacII CCGCGG 1 cut(s) 193
SaqAI TTAA 3 cut(s) 333, 516, 1157
Sau3AI GATC 5 cut(s) 267, 786, 882, 927, 963
Sau96I GGNCC 3 cut(s) 308, 946, 1133
ScaI AGTACT 1 cut(s) 751
SchI GAGTC 1 cut(s) 823
ScrFI CCNGG 3 cut(s) 130, 370, 660
SduI GDGCHC 3 cut(s) 87, 137, 802
SfaNI GCATC 2 cut(s) 619, 855
Sfr303I CCGCGG 1 cut(s) 193
SfuI TTCGAA 2 cut(s) 461, 476
SgrBI CCGCGG 1 cut(s) 193
SinI GGWCC 2 cut(s) 308, 1133
SmlI CTYRAG 4 cut(s) 66, 583, 967, 1077
SmoI CTYRAG 4 cut(s) 66, 583, 967, 1077
Sse9I AATT 1 cut(s) 870
SspMI CTAG 1 cut(s) 915
SstI GAGCTC 3 cut(s) 87, 137, 802
StyD4I CCNGG 3 cut(s) 128, 368, 658
StyI CCWWGG 2 cut(s) 490, 860
TaaI ACNGT 4 cut(s) 322, 602, 675, 1164
TaiI ACGT 3 cut(s) 99, 125, 207
TaqI TCGA 2 cut(s) 461, 476
TasI AATT 1 cut(s) 870
TatI WGTACW 3 cut(s) 749, 889, 1104
TauI GCSGC 7 cut(s) 64, 189, 192, 195, 198, 423, 610
TfiI GAWTC 6 cut(s) 71, 104, 149, 557, 701, 841
Tru1I TTAA 3 cut(s) 333, 516, 1157
Tru9I TTAA 3 cut(s) 333, 516, 1157
TscAI CASTG 2 cut(s) 509, 1169
TseFI GTSAC 3 cut(s) 92, 178, 200
TseI GCWGC 3 cut(s) 174, 655, 1015
Tsp45I GTSAC 3 cut(s) 92, 178, 200
TspDTI ATGAA 3 cut(s) 8, 609, 773
TspGWI ACGGA 2 cut(s) 1098, 1146
TspRI CASTG 2 cut(s) 509, 1169
VpaK11BI GGWCC 2 cut(s) 308, 1133
VspI ATTAAT 2 cut(s) 333, 516
XcmI CCANNNNNNNNNTGG 1 cut(s) 858
XmiI GTMKAC 2 cut(s) 390, 1128
XmnI GAANNNNTTC 1 cut(s) 818
XspI CTAG 1 cut(s) 915
ZraI GACGTC 2 cut(s) 123, 205
ZrmI AGTACT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.