Rroxscaffold_3G00262020
NAC Family

inactive poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007192
Physical Location & Seq
Reverse (-)
56125235 .. 56130937
5703 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262020.1

Sequence Viewer

Length: 1170 bp
ATGGAATACAGCGGTGTTCAACGTCCGTTATCCTCTGTTTTCGCCGATGGGTTCAAAGCCTCACCGCCGCCGCCGCAGCCTAACGATTGCGAACTCTCCGATCAGGTTTCCGATTCCGTCACCACAGAGACGTCCTCAGGGAGCTCCGACGATCAAGAGTCATCGGTCTCCGATTGCGAAAGCAGCGTCACGAGCCGCGGCGACGACCGTCGCCGGGTGTTGGACGCCGGATTAGTGAAACTTGGAGAAGGGGACAGAGTCCACGACCTCATCAAGCAGAGGTTTCTTGCGAGTTTGGGTTTGCTTGGAGCGCACGTCACCGTTACGGCGATTAATAGAAACTCCCATTCGGGTTTTACGGGTCAAGCGCGGCTGCACGCGTTTCGGGTTTACTTGAAAGCTGTGGAGGACAAGTGTGGTGGAAACGCCAATGTGAAATATGGTTGGTATTCTCCGAGTTCGAAAGCCGAGATTTCGAACATCATTGGCCATGGATTTGGTCACTGTGAAAAGATCAACGGGGTTTATGGTAGAGGTGTCTATCTTGCTCCTGATGATTCACCTTTGGAATGTTTGGAAAACTTGAGTGTTGATGAAGACGGTCTTCGCCATCTTTTGCTTTGCCGTGTGATTTTGGGGAAGCCGGAAGTTGTGCTGCCTGGTAATTCTGAACAGTATCATCCCAGTTCGCAAGAGTTTGATTCGGGTGTGGACAGGCTTTTCGCTCCCAAGAAGTATATAGTGTGGAGTACTCACATGAACACCCATATCTTGCCGGAGTATGTCATCAGCTTCAGAGCTCCTTCTTGCCTGAAAGAGTTCTTGAAGACTCAGGAACCAATTAGAAAACCAACTTCACCATGGATGCCAATTCCAACTCTAATCAGTGTGCTTTCGAAGTTCTTGCCTCAGCCTAGCATTGCTTTGATCATCAAGTATCATAAAGCCCACAGAGAAACGAAGATCTCAAGGAGTGAACTAATACAGAGAATTAGACAAATAGCAGGAGACAAGTTGCTGGCTACAATAATCAAATCTTTCAGAACCAAGCAACTCAAACCTTCGCAAGGGGTTTCTCAGGACGGAGGCAGGAATGCGATGGAGTACAAAAATAATGCAGGCGGTTTAGACGGACCTATGCTTTTTGGAGTATACTTAAAACAGATCTAA

Protein Analysis

389

Amino Acids

42.88

Weight (kDa)

8.36

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 283 - 348 2.8e-24 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 134
AccI GTMKAC 1 cut(s) 1152
AccII CGCG 3 cut(s) 198, 370, 380
AciI CCGC 9 cut(s) 12, 65, 68, 71, 74, 196, 198, 370, 1122
AcoI YGGCCR 1 cut(s) 487
AcuI CTGAAG 1 cut(s) 778
AcyI GRCGYC 2 cut(s) 131, 225
AfaI GTAC 2 cut(s) 751, 1106
AfiI CCNNNNNNNGG 3 cut(s) 214, 220, 1067
AflIII ACRYGT 1 cut(s) 378
AgsI TTSAA 4 cut(s) 20, 55, 397, 826
AjiI CACGTC 1 cut(s) 316
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 4 cut(s) 144, 401, 792, 800
AluI AGCT 4 cut(s) 144, 401, 792, 800
Alw21I GWGCWC 2 cut(s) 146, 802
Alw26I GTCTC 3 cut(s) 122, 172, 1002
AoxI GGCC 1 cut(s) 487
ApeKI GCWGC 4 cut(s) 76, 183, 373, 655
ArsI GACNNNNNNTTYG 4 cut(s) 171, 203, 704, 736
AseI ATTAAT 1 cut(s) 333
Asp700I GAANNNNTTC 1 cut(s) 818
AspLEI GCGC 2 cut(s) 313, 370
AspS9I GGNCC 1 cut(s) 1133
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 5 cut(s) 54, 112, 310, 552, 849
AsuII TTCGAA 3 cut(s) 461, 476, 896
AvaII GGWCC 1 cut(s) 1133
AxyI CCTNAGG 1 cut(s) 136
BalI TGGCCA 1 cut(s) 489
BanII GRGCYC 2 cut(s) 146, 802
BauI CACGAG 1 cut(s) 190
BbsI GAAGAC 3 cut(s) 596, 603, 833
Bbv12I GWGCWC 2 cut(s) 146, 802
BbvCI CCTCAGC 1 cut(s) 909
BbvI GCAGC 4 cut(s) 88, 195, 360, 642
BccI CCATC 3 cut(s) 41, 618, 1093
BceAI ACGGC 2 cut(s) 342, 609
BcgI CGANNNNNNTGC 4 cut(s) 365, 399, 886, 920
BciT130I CCWGG 1 cut(s) 660
BclI TGATCA 1 cut(s) 927
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 3 cut(s) 122, 172, 1002
BfaI CTAG 1 cut(s) 915
BglII AGATCT 2 cut(s) 963, 1164
BmcAI AGTACT 1 cut(s) 751
Bme1390I CCNGG 2 cut(s) 215, 660
Bme18I GGWCC 1 cut(s) 1133
BmgBI CACGTC 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 1133
BmiI GGNNCC 1 cut(s) 837
BmrFI CCNGG 2 cut(s) 215, 660
BmrI ACTGGG 1 cut(s) 678
BmsI GCATC 1 cut(s) 855
BmuI ACTGGG 1 cut(s) 678
BoxI GACNNNNGTC 1 cut(s) 207
BpiI GAAGAC 3 cut(s) 596, 603, 833
BplI GAGNNNNNCTC 2 cut(s) 119, 151
Bpu10I CCTNAGC 1 cut(s) 909
Bpu14I TTCGAA 3 cut(s) 461, 476, 896
BpuEI CTTGAG 2 cut(s) 604, 952
BpuMI CCSGG 1 cut(s) 215
BsaHI GRCGYC 2 cut(s) 131, 225
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 3 cut(s) 196, 490, 860
BsaXI ACNNNNNCTCC 4 cut(s) 300, 330, 398, 428
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 220, 1067
Bse1I ACTGG 1 cut(s) 684
Bse21I CCTNAGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 918
BseBI CCWGG 1 cut(s) 660
BseDI CCNNGG 3 cut(s) 196, 490, 860
BseGI GGATG 2 cut(s) 679, 870
BseLI CCNNNNNNNGG 3 cut(s) 214, 220, 1067
BseMI GCAATG 1 cut(s) 918
BseMII CTCAG 4 cut(s) 150, 845, 923, 1091
BseNI ACTGG 1 cut(s) 684
BseXI GCAGC 4 cut(s) 88, 195, 360, 642
BsgI GTGCAG 1 cut(s) 359
Bsh1236I CGCG 3 cut(s) 198, 370, 380
Bsh1285I CGRYCG 1 cut(s) 208
BshFI GGCC 1 cut(s) 489
BsiEI CGRYCG 1 cut(s) 208
BsiHKAI GWGCWC 2 cut(s) 146, 802
BsiSI CCGG 4 cut(s) 214, 228, 644, 776
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 3 cut(s) 214, 220, 1067
BsmAI GTCTC 3 cut(s) 122, 172, 1002
BsmBI CGTCTC 1 cut(s) 122
BsmFI GGGAC 1 cut(s) 266
BsmI GAATGC 1 cut(s) 1099
BsnI GGCC 1 cut(s) 489
Bso31I GGTCTC 1 cut(s) 172
Bsp119I TTCGAA 3 cut(s) 461, 476, 896
Bsp1286I GDGCHC 2 cut(s) 146, 802
Bsp143I GATC 6 cut(s) 100, 151, 513, 927, 963, 1164
Bsp19I CCATGG 2 cut(s) 490, 860
BspACI CCGC 9 cut(s) 12, 65, 68, 71, 74, 196, 198, 370, 1122
BspANI GGCC 1 cut(s) 489
BspCNI CTCAG 4 cut(s) 149, 844, 922, 1090
BspFNI CGCG 3 cut(s) 198, 370, 380
BspLI GGNNCC 1 cut(s) 837
BspT104I TTCGAA 3 cut(s) 461, 476, 896
BspTNI GGTCTC 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 918
BsrI ACTGG 1 cut(s) 684
BssECI CCNNGG 3 cut(s) 196, 490, 860
BssMI GATC 6 cut(s) 100, 151, 513, 927, 963, 1164
BssNAI GTATAC 1 cut(s) 1153
BssNI GRCGYC 2 cut(s) 131, 225
BssSI CACGAG 1 cut(s) 190
BssT1I CCWWGG 2 cut(s) 490, 860
Bst1107I GTATAC 1 cut(s) 1153
Bst2BI CACGAG 1 cut(s) 190
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 5 cut(s) 209, 322, 506, 602, 675
BstACI GRCGYC 2 cut(s) 131, 225
BstBI TTCGAA 3 cut(s) 461, 476, 896
BstC8I GCNNGC 3 cut(s) 378, 1020, 1120
BstDEI CTNAG 4 cut(s) 136, 831, 909, 1077
BstDSI CCRYGG 3 cut(s) 196, 490, 860
BstENI CCTNNNNNAGG 1 cut(s) 1065
BstF5I GGATG 2 cut(s) 679, 870
BstFNI CGCG 3 cut(s) 198, 370, 380
BstHHI GCGC 2 cut(s) 313, 370
BstKTI GATC 6 cut(s) 103, 154, 516, 930, 966, 1167
BstMAI GTCTC 3 cut(s) 122, 172, 1002
BstMBI GATC 6 cut(s) 100, 151, 513, 927, 963, 1164
BstMCI CGRYCG 1 cut(s) 208
BstMWI GCNNNNNNNGC 5 cut(s) 73, 76, 183, 192, 310
BstNI CCWGG 1 cut(s) 660
BstPAI GACNNNNGTC 1 cut(s) 207
BstSCI CCNGG 2 cut(s) 213, 658
BstUI CGCG 3 cut(s) 198, 370, 380
BstV1I GCAGC 4 cut(s) 88, 195, 360, 642
BstV2I GAAGAC 3 cut(s) 596, 603, 833
BstX2I RGATCY 2 cut(s) 963, 1164
BstXI CCANNNNNNTGG 1 cut(s) 497
BstYI RGATCY 2 cut(s) 963, 1164
BstZ17I GTATAC 1 cut(s) 1153
Bsu36I CCTNAGG 1 cut(s) 136
BsuRI GGCC 1 cut(s) 489
BtgI CCRYGG 3 cut(s) 196, 490, 860
BtgZI GCGATG 1 cut(s) 1112
BtrI CACGTC 1 cut(s) 316
BtsCI GGATG 2 cut(s) 679, 870
BtsIMutI CAGTG 2 cut(s) 502, 892
Cac8I GCNNGC 3 cut(s) 378, 1020, 1120
CfoI GCGC 2 cut(s) 313, 370
Cfr13I GGNCC 1 cut(s) 1133
Cfr42I CCGCGG 1 cut(s) 199
CseI GACGC 2 cut(s) 175, 233
Csp6I GTAC 2 cut(s) 750, 1105
CspCI CAANNNNNGTGG 2 cut(s) 400, 435
CviAII CATG 3 cut(s) 491, 757, 861
CviQI GTAC 2 cut(s) 750, 1105
DdeI CTNAG 4 cut(s) 136, 831, 909, 1077
DpnI GATC 6 cut(s) 102, 153, 515, 929, 965, 1166
DpnII GATC 6 cut(s) 100, 151, 513, 927, 963, 1164
EaeI YGGCCR 1 cut(s) 487
Ecl136II GAGCTC 2 cut(s) 144, 800
Eco130I CCWWGG 2 cut(s) 490, 860
Eco24I GRGCYC 2 cut(s) 146, 802
Eco31I GGTCTC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 1133
Eco53kI GAGCTC 2 cut(s) 144, 800
Eco57I CTGAAG 1 cut(s) 778
Eco81I CCTNAGG 1 cut(s) 136
EcoICRI GAGCTC 2 cut(s) 144, 800
EcoNI CCTNNNNNAGG 1 cut(s) 1065
EcoRII CCWGG 1 cut(s) 658
EcoT14I CCWWGG 2 cut(s) 490, 860
EcoT38I GRGCYC 2 cut(s) 146, 802
ErhI CCWWGG 2 cut(s) 490, 860
Esp3I CGTCTC 1 cut(s) 122
FaeI CATG 3 cut(s) 494, 760, 864
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FaqI GGGAC 1 cut(s) 266
FatI CATG 3 cut(s) 490, 756, 860
FbaI TGATCA 1 cut(s) 927
FblI GTMKAC 1 cut(s) 1152
FokI GGATG 2 cut(s) 666, 877
FriOI GRGCYC 2 cut(s) 146, 802
FspBI CTAG 1 cut(s) 915
GlaI GCGC 2 cut(s) 312, 369
HaeIII GGCC 1 cut(s) 489
HapII CCGG 4 cut(s) 214, 228, 644, 776
HgaI GACGC 2 cut(s) 175, 233
HhaI GCGC 2 cut(s) 313, 370
Hin1I GRCGYC 2 cut(s) 131, 225
Hin1II CATG 3 cut(s) 494, 760, 864
Hin6I GCGC 2 cut(s) 311, 368
HinP1I GCGC 2 cut(s) 311, 368
HinfI GANTC 6 cut(s) 113, 158, 258, 557, 701, 829
HpaII CCGG 4 cut(s) 214, 228, 644, 776
HphI GGTGA 5 cut(s) 54, 112, 310, 552, 849
Hpy166II GTNNAC 5 cut(s) 262, 391, 712, 977, 1153
Hpy188I TCNGA 8 cut(s) 100, 112, 148, 172, 456, 670, 797, 1043
Hpy188III TCNNGA 6 cut(s) 155, 190, 551, 823, 833, 1079
Hpy8I GTNNAC 5 cut(s) 262, 391, 712, 977, 1153
Hpy99I CGWCG 3 cut(s) 152, 206, 213
HpyAV CCTTC 3 cut(s) 242, 813, 1071
HpyCH4III ACNGT 5 cut(s) 209, 322, 506, 602, 675
HpyCH4IV ACGT 3 cut(s) 22, 131, 315
HpyCH4V TGCA 2 cut(s) 376, 1118
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 76, 183, 192, 310
HpyF3I CTNAG 4 cut(s) 136, 831, 909, 1077
HpySE526I ACGT 3 cut(s) 22, 131, 315
Hsp92I GRCGYC 2 cut(s) 131, 225
Hsp92II CATG 3 cut(s) 494, 760, 864
HspAI GCGC 2 cut(s) 311, 368
Ksp22I TGATCA 1 cut(s) 927
KspI CCGCGG 1 cut(s) 199
Kzo9I GATC 6 cut(s) 100, 151, 513, 927, 963, 1164
LmnI GCTCC 6 cut(s) 141, 149, 308, 553, 730, 805
Lsp1109I GCAGC 4 cut(s) 88, 195, 360, 642
LweI GCATC 1 cut(s) 855
MaeI CTAG 1 cut(s) 915
MaeII ACGT 3 cut(s) 22, 131, 315
MaeIII GTNAC 5 cut(s) 118, 187, 316, 322, 500
MalI GATC 6 cut(s) 102, 153, 515, 929, 965, 1166
MboI GATC 6 cut(s) 100, 151, 513, 927, 963, 1164
MboII GAAGA 4 cut(s) 596, 608, 838, 973
MflI RGATCY 2 cut(s) 963, 1164
MhlI GDGCHC 2 cut(s) 146, 802
MlsI TGGCCA 1 cut(s) 489
MluCI AATT 4 cut(s) 664, 840, 870, 990
MluI ACGCGT 1 cut(s) 378
MluNI TGGCCA 1 cut(s) 489
MlyI GAGTC 3 cut(s) 167, 267, 823
MmeI TCCRAC 3 cut(s) 171, 201, 899
MnlI CCTC 9 cut(s) 43, 70, 145, 273, 278, 400, 527, 918, 1079
Mox20I TGGCCA 1 cut(s) 489
MroXI GAANNNNTTC 1 cut(s) 818
MscI TGGCCA 1 cut(s) 489
MseI TTAA 2 cut(s) 333, 1157
Msp20I TGGCCA 1 cut(s) 489
MspA1I CMGCKG 2 cut(s) 12, 198
MspI CCGG 4 cut(s) 214, 228, 644, 776
MspR9I CCNGG 2 cut(s) 215, 660
Mva1269I GAATGC 1 cut(s) 1099
MvaI CCWGG 1 cut(s) 660
MvnI CGCG 3 cut(s) 198, 370, 380
MwoI GCNNNNNNNGC 5 cut(s) 73, 76, 183, 192, 310
NciI CCSGG 1 cut(s) 215
NcoI CCATGG 2 cut(s) 490, 860
NdeII GATC 6 cut(s) 100, 151, 513, 927, 963, 1164
NlaIII CATG 3 cut(s) 494, 760, 864
NlaIV GGNNCC 1 cut(s) 837
NmeAIII GCCGAG 1 cut(s) 493
NmuCI GTSAC 4 cut(s) 118, 187, 316, 500
NspV TTCGAA 3 cut(s) 461, 476, 896
PctI GAATGC 1 cut(s) 1099
PdmI GAANNNNTTC 1 cut(s) 818
PfeI GAWTC 3 cut(s) 113, 557, 701
PflFI GACNNNGTC 1 cut(s) 257
PleI GAGTC 3 cut(s) 166, 266, 823
PpsI GAGTC 3 cut(s) 166, 266, 823
PshAI GACNNNNGTC 1 cut(s) 207
PshBI ATTAAT 1 cut(s) 333
Psp124BI GAGCTC 2 cut(s) 146, 802
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 1 cut(s) 837
PspPI GGNCC 1 cut(s) 1133
PsuI RGATCY 2 cut(s) 963, 1164
PsyI GACNNNGTC 1 cut(s) 257
RsaI GTAC 2 cut(s) 751, 1106
RsaNI GTAC 2 cut(s) 750, 1105
SacI GAGCTC 2 cut(s) 146, 802
SacII CCGCGG 1 cut(s) 199
SaqAI TTAA 2 cut(s) 333, 1157
Sau3AI GATC 6 cut(s) 100, 151, 513, 927, 963, 1164
Sau96I GGNCC 1 cut(s) 1133
ScaI AGTACT 1 cut(s) 751
SchI GAGTC 3 cut(s) 167, 267, 823
ScrFI CCNGG 2 cut(s) 215, 660
SduI GDGCHC 2 cut(s) 146, 802
SfaNI GCATC 1 cut(s) 855
Sfr303I CCGCGG 1 cut(s) 199
SfuI TTCGAA 3 cut(s) 461, 476, 896
SgrBI CCGCGG 1 cut(s) 199
SinI GGWCC 1 cut(s) 1133
SmlI CTYRAG 2 cut(s) 583, 967
SmoI CTYRAG 2 cut(s) 583, 967
Sse9I AATT 4 cut(s) 664, 840, 870, 990
SsiI CCGC 9 cut(s) 12, 65, 68, 71, 74, 196, 198, 370, 1122
SspMI CTAG 1 cut(s) 915
SstI GAGCTC 2 cut(s) 146, 802
StyD4I CCNGG 2 cut(s) 213, 658
StyI CCWWGG 2 cut(s) 490, 860
TaaI ACNGT 5 cut(s) 209, 322, 506, 602, 675
TaiI ACGT 3 cut(s) 25, 134, 318
TaqI TCGA 3 cut(s) 461, 476, 896
TaqII GACCGA 1 cut(s) 154
TasI AATT 4 cut(s) 664, 840, 870, 990
TatI WGTACW 2 cut(s) 749, 1104
TauI GCSGC 6 cut(s) 70, 73, 76, 198, 201, 373
TfiI GAWTC 3 cut(s) 113, 557, 701
Tru1I TTAA 2 cut(s) 333, 1157
Tru9I TTAA 2 cut(s) 333, 1157
TscAI CASTG 2 cut(s) 509, 892
TseFI GTSAC 4 cut(s) 118, 187, 316, 500
TseI GCWGC 4 cut(s) 76, 183, 373, 655
Tsp45I GTSAC 4 cut(s) 118, 187, 316, 500
TspDTI ATGAA 2 cut(s) 609, 773
TspGWI ACGGA 4 cut(s) 15, 106, 1098, 1146
TspRI CASTG 2 cut(s) 509, 892
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 1133
VspI ATTAAT 1 cut(s) 333
XagI CCTNNNNNAGG 1 cut(s) 1065
XcmI CCANNNNNNNNNTGG 1 cut(s) 858
XmiI GTMKAC 1 cut(s) 1152
XmnI GAANNNNTTC 1 cut(s) 818
XspI CTAG 1 cut(s) 915
ZraI GACGTC 1 cut(s) 132
ZrmI AGTACT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.