Rw7G011660
NAC Family

inactive poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007192
Physical Location & Seq
Forward (+)
10963000 .. 10966091
3092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G011660.1

Sequence Viewer

Length: 1176 bp
ATGGAATACAGTGGTGTTCAACGTCCGTTATCCTCTGTTTTCTCCGATGGGTTCACAGCCTCGCTGCCGCCGCCTCAGCCGCCTAACGATTGCGAACTCTCCGATCAGGTTTCCGATTCCGTCACCACAGAGACGTCCTCAGGGAGCTCCGACGATCAAGAATCATCTGTCTCCGATTGCGAAAGCAGCGTCACGAGCCGCGGAGGCGACGACCGTCGCCGGGTGTTGGACGCCGGGTTAGTGAAACTTGGAGAAGGGGACAGAGTCCACGACCTCATCAAGCAAAGGTTTCTTGCGAGTTTGGGATTGCTTGGAGCGCACGTCACCGTTACGGCGATTAATAGAAACTCCCATTCGGGTTTTACGGGTCAAGCGCGGCTGCACGCGTTTCGGGTTTACTTGAAAGCTGTGGAGGACAAGTGTGGTGGAAACGCCAATGTGAAATATGGTTGGTATTCTCCGAGTTCGAAAGCCGAGATCTCGAAGATCATTGGCCATGGATTTGGTCACTGTGAAAAGATCAACGGGGTTTATGGTAGAGGTGTCTATCTTGCTCCTGATGATTCACCTTTGGAATGTTTGGAAAACTTGAGTGAAGATGAAGACGGTCTTCGGCATCTTTTGCTTTGCCGTGTGATTTTGGGGAAGCCGGAAGTTGTGCTGCCTGGTAATTCTGAACAGTATCATCCCAGTTCTCAAGAGTTTGATTCGGGTGTGGACAGGCTTTTCGCTCCCAAGAAGTATATAGTGTGGAGTACTCACATGAACACTCATATCTTGCCGGAGTATGTCATCAGCTTCAGAGCTCCTACTTGCCTGAAAGAGTTCTTGAAGACTCGGGAACCAATTAGAAAACCAACTTCACCATGGATGCCAATTCCAACTCTAATCAGTGTGCTTTCCAAGTTCTTGCCTCAGCCTAGCATTGCTTTGATCATCAAGTATCATAAAGCCCACAGAGAAACGAAGATCTCAAGGAGTGAACTAATACAGAGAATTAGACAAATAGCAGGAGACAAGTTGCTGGCTACAATCATCAAATCTTTCAGAACCAAGCAACTCAAACCTTCGCAAGGGGTTTCTCAGGACGGAGGCAGGAATGCGATGGAGTACAAAAATAATGCAGGCGGTTTAGACGGACCGATGCTTTTTGGAGTAAACTTAAAACAGATCTAA

Protein Analysis

391

Amino Acids

43.08

Weight (kDa)

8.36

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 285 - 350 2.8e-24 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 137
AccII CGCG 3 cut(s) 201, 376, 386
AciI CCGC 7 cut(s) 68, 71, 80, 199, 201, 376, 1128
AcoI YGGCCR 1 cut(s) 493
AcuI CTGAAG 1 cut(s) 784
AcyI GRCGYC 2 cut(s) 134, 231
AfaI GTAC 2 cut(s) 757, 1112
AfiI CCNNNNNNNGG 3 cut(s) 220, 226, 1073
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 3 cut(s) 20, 403, 832
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 1 cut(s) 664
AluBI AGCT 4 cut(s) 147, 407, 798, 806
AluI AGCT 4 cut(s) 147, 407, 798, 806
Alw21I GWGCWC 2 cut(s) 149, 808
Alw26I GTCTC 3 cut(s) 125, 175, 1008
Ama87I CYCGRG 1 cut(s) 837
AoxI GGCC 1 cut(s) 493
ApeKI GCWGC 4 cut(s) 64, 186, 379, 661
ArsI GACNNNNNNTTYG 4 cut(s) 174, 206, 710, 742
AseI ATTAAT 1 cut(s) 339
Asp700I GAANNNNTTC 1 cut(s) 824
AspLEI GCGC 2 cut(s) 319, 376
AspS9I GGNCC 1 cut(s) 1139
AsuC2I CCSGG 2 cut(s) 221, 235
AsuHPI GGTGA 4 cut(s) 115, 316, 558, 855
AsuII TTCGAA 1 cut(s) 467
AvaI CYCGRG 1 cut(s) 837
AvaII GGWCC 1 cut(s) 1139
AxyI CCTNAGG 1 cut(s) 139
BalI TGGCCA 1 cut(s) 495
BanII GRGCYC 2 cut(s) 149, 808
BauI CACGAG 1 cut(s) 193
BbsI GAAGAC 3 cut(s) 602, 609, 839
Bbv12I GWGCWC 2 cut(s) 149, 808
BbvCI CCTCAGC 2 cut(s) 75, 915
BbvI GCAGC 4 cut(s) 51, 198, 366, 648
BccI CCATC 2 cut(s) 41, 1099
BceAI ACGGC 2 cut(s) 348, 615
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BciT130I CCWGG 1 cut(s) 666
BclI TGATCA 1 cut(s) 933
BcnI CCSGG 2 cut(s) 221, 235
BcoDI GTCTC 3 cut(s) 125, 175, 1008
BfaI CTAG 1 cut(s) 921
BglI GCCNNNNNGGC 1 cut(s) 204
BglII AGATCT 3 cut(s) 477, 969, 1170
BisI GCNGC 9 cut(s) 65, 68, 71, 80, 187, 199, 377, 380, 662
BlsI GCNGC 9 cut(s) 66, 69, 72, 81, 188, 200, 378, 381, 663
BmcAI AGTACT 1 cut(s) 757
Bme1390I CCNGG 3 cut(s) 221, 235, 666
Bme18I GGWCC 1 cut(s) 1139
BmeT110I CYCGRG 1 cut(s) 837
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 1139
BmiI GGNNCC 1 cut(s) 843
BmrFI CCNGG 3 cut(s) 221, 235, 666
BmrI ACTGGG 1 cut(s) 684
BmsI GCATC 3 cut(s) 625, 861, 1134
BmuI ACTGGG 1 cut(s) 684
BoxI GACNNNNGTC 1 cut(s) 213
BpiI GAAGAC 3 cut(s) 602, 609, 839
BplI GAGNNNNNCTC 2 cut(s) 122, 154
Bpu10I CCTNAGC 2 cut(s) 75, 915
Bpu14I TTCGAA 1 cut(s) 467
BpuEI CTTGAG 3 cut(s) 610, 681, 958
BpuMI CCSGG 2 cut(s) 221, 235
BsaHI GRCGYC 2 cut(s) 134, 231
BsaJI CCNNGG 3 cut(s) 199, 496, 866
BsaXI ACNNNNNCTCC 4 cut(s) 306, 336, 404, 434
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 226, 1073
Bse1I ACTGG 1 cut(s) 690
Bse21I CCTNAGG 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 924
BseBI CCWGG 1 cut(s) 666
BseDI CCNNGG 3 cut(s) 199, 496, 866
BseGI GGATG 2 cut(s) 685, 876
BseLI CCNNNNNNNGG 3 cut(s) 220, 226, 1073
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 4 cut(s) 89, 153, 929, 1097
BseNI ACTGG 1 cut(s) 690
BseXI GCAGC 4 cut(s) 51, 198, 366, 648
BsgI GTGCAG 1 cut(s) 365
Bsh1236I CGCG 3 cut(s) 201, 376, 386
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 1 cut(s) 495
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 2 cut(s) 149, 808
BsiHKCI CYCGRG 1 cut(s) 837
BsiSI CCGG 4 cut(s) 220, 234, 650, 782
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 3 cut(s) 220, 226, 1073
BsmAI GTCTC 3 cut(s) 125, 175, 1008
BsmBI CGTCTC 1 cut(s) 125
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 1105
BsnI GGCC 1 cut(s) 495
BsoBI CYCGRG 1 cut(s) 837
Bsp119I TTCGAA 1 cut(s) 467
Bsp1286I GDGCHC 2 cut(s) 149, 808
Bsp143I GATC 8 cut(s) 103, 154, 477, 486, 519, 933, 969, 1170
Bsp19I CCATGG 2 cut(s) 496, 866
BspACI CCGC 7 cut(s) 68, 71, 80, 199, 201, 376, 1128
BspANI GGCC 1 cut(s) 495
BspCNI CTCAG 4 cut(s) 88, 152, 928, 1096
BspFNI CGCG 3 cut(s) 201, 376, 386
BspLI GGNNCC 1 cut(s) 843
BspT104I TTCGAA 1 cut(s) 467
BsrDI GCAATG 1 cut(s) 924
BsrI ACTGG 1 cut(s) 690
BssECI CCNNGG 3 cut(s) 199, 496, 866
BssMI GATC 8 cut(s) 103, 154, 477, 486, 519, 933, 969, 1170
BssNI GRCGYC 2 cut(s) 134, 231
BssSI CACGAG 1 cut(s) 193
BssT1I CCWWGG 2 cut(s) 496, 866
Bst2BI CACGAG 1 cut(s) 193
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 6 cut(s) 11, 215, 328, 512, 608, 681
BstACI GRCGYC 2 cut(s) 134, 231
BstAPI GCANNNNNTGC 1 cut(s) 622
BstBI TTCGAA 1 cut(s) 467
BstC8I GCNNGC 3 cut(s) 384, 1026, 1126
BstDEI CTNAG 4 cut(s) 75, 139, 915, 1083
BstDSI CCRYGG 3 cut(s) 199, 496, 866
BstENI CCTNNNNNAGG 1 cut(s) 1071
BstF5I GGATG 2 cut(s) 685, 876
BstFNI CGCG 3 cut(s) 201, 376, 386
BstHHI GCGC 2 cut(s) 319, 376
BstKTI GATC 8 cut(s) 106, 157, 480, 489, 522, 936, 972, 1173
BstMAI GTCTC 3 cut(s) 125, 175, 1008
BstMBI GATC 8 cut(s) 103, 154, 477, 486, 519, 933, 969, 1170
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 8 cut(s) 70, 76, 79, 186, 195, 204, 316, 622
BstNI CCWGG 1 cut(s) 666
BstPAI GACNNNNGTC 1 cut(s) 213
BstSCI CCNGG 3 cut(s) 219, 233, 664
BstUI CGCG 3 cut(s) 201, 376, 386
BstV1I GCAGC 4 cut(s) 51, 198, 366, 648
BstV2I GAAGAC 3 cut(s) 602, 609, 839
BstX2I RGATCY 3 cut(s) 477, 969, 1170
BstXI CCANNNNNNTGG 1 cut(s) 503
BstYI RGATCY 3 cut(s) 477, 969, 1170
Bsu36I CCTNAGG 1 cut(s) 139
BsuRI GGCC 1 cut(s) 495
BtgI CCRYGG 3 cut(s) 199, 496, 866
BtgZI GCGATG 1 cut(s) 1118
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 2 cut(s) 685, 876
BtsIMutI CAGTG 3 cut(s) 16, 508, 898
Cac8I GCNNGC 3 cut(s) 384, 1026, 1126
CfoI GCGC 2 cut(s) 319, 376
Cfr13I GGNCC 1 cut(s) 1139
Cfr42I CCGCGG 1 cut(s) 202
CpoI CGGWCCG 1 cut(s) 1139
CseI GACGC 2 cut(s) 178, 239
Csp6I GTAC 2 cut(s) 756, 1111
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CspI CGGWCCG 1 cut(s) 1139
CviAII CATG 3 cut(s) 497, 763, 867
CviQI GTAC 2 cut(s) 756, 1111
DdeI CTNAG 4 cut(s) 75, 139, 915, 1083
DpnI GATC 8 cut(s) 105, 156, 479, 488, 521, 935, 971, 1172
DpnII GATC 8 cut(s) 103, 154, 477, 486, 519, 933, 969, 1170
EaeI YGGCCR 1 cut(s) 493
Ecl136II GAGCTC 2 cut(s) 147, 806
Eco130I CCWWGG 2 cut(s) 496, 866
Eco24I GRGCYC 2 cut(s) 149, 808
Eco47I GGWCC 1 cut(s) 1139
Eco53kI GAGCTC 2 cut(s) 147, 806
Eco57I CTGAAG 1 cut(s) 784
Eco81I CCTNAGG 1 cut(s) 139
Eco88I CYCGRG 1 cut(s) 837
EcoICRI GAGCTC 2 cut(s) 147, 806
EcoNI CCTNNNNNAGG 1 cut(s) 1071
EcoRII CCWGG 1 cut(s) 664
EcoT14I CCWWGG 2 cut(s) 496, 866
EcoT38I GRGCYC 2 cut(s) 149, 808
ErhI CCWWGG 2 cut(s) 496, 866
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 3 cut(s) 500, 766, 870
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FaqI GGGAC 1 cut(s) 272
FatI CATG 3 cut(s) 496, 762, 866
FbaI TGATCA 1 cut(s) 933
Fnu4HI GCNGC 9 cut(s) 65, 68, 71, 80, 187, 199, 377, 380, 662
FokI GGATG 2 cut(s) 672, 883
FriOI GRGCYC 2 cut(s) 149, 808
Fsp4HI GCNGC 9 cut(s) 65, 68, 71, 80, 187, 199, 377, 380, 662
FspBI CTAG 1 cut(s) 921
GlaI GCGC 2 cut(s) 318, 375
GluI GCNGC 9 cut(s) 65, 68, 71, 80, 187, 199, 377, 380, 662
HaeIII GGCC 1 cut(s) 495
HapII CCGG 4 cut(s) 220, 234, 650, 782
HgaI GACGC 2 cut(s) 178, 239
HhaI GCGC 2 cut(s) 319, 376
Hin1I GRCGYC 2 cut(s) 134, 231
Hin1II CATG 3 cut(s) 500, 766, 870
Hin6I GCGC 2 cut(s) 317, 374
HinP1I GCGC 2 cut(s) 317, 374
HinfI GANTC 6 cut(s) 116, 161, 264, 563, 707, 835
HpaII CCGG 4 cut(s) 220, 234, 650, 782
HphI GGTGA 4 cut(s) 115, 316, 558, 855
Hpy166II GTNNAC 6 cut(s) 54, 268, 397, 718, 983, 1159
Hpy188I TCNGA 9 cut(s) 46, 103, 115, 151, 175, 462, 676, 803, 1049
Hpy188III TCNNGA 8 cut(s) 158, 193, 481, 557, 698, 829, 839, 1085
Hpy8I GTNNAC 6 cut(s) 54, 268, 397, 718, 983, 1159
Hpy99I CGWCG 3 cut(s) 155, 212, 219
HpyAV CCTTC 2 cut(s) 248, 1077
HpyCH4III ACNGT 6 cut(s) 11, 215, 328, 512, 608, 681
HpyCH4IV ACGT 3 cut(s) 22, 134, 321
HpyCH4V TGCA 2 cut(s) 382, 1124
HpyF10VI GCNNNNNNNGC 8 cut(s) 70, 76, 79, 186, 195, 204, 316, 622
HpyF3I CTNAG 4 cut(s) 75, 139, 915, 1083
HpySE526I ACGT 3 cut(s) 22, 134, 321
Hsp92I GRCGYC 2 cut(s) 134, 231
Hsp92II CATG 3 cut(s) 500, 766, 870
HspAI GCGC 2 cut(s) 317, 374
Ksp22I TGATCA 1 cut(s) 933
KspI CCGCGG 1 cut(s) 202
Kzo9I GATC 8 cut(s) 103, 154, 477, 486, 519, 933, 969, 1170
LmnI GCTCC 6 cut(s) 144, 152, 314, 559, 736, 811
Lsp1109I GCAGC 4 cut(s) 51, 198, 366, 648
LweI GCATC 3 cut(s) 625, 861, 1134
MaeI CTAG 1 cut(s) 921
MaeII ACGT 3 cut(s) 22, 134, 321
MaeIII GTNAC 5 cut(s) 121, 190, 322, 328, 506
MalI GATC 8 cut(s) 105, 156, 479, 488, 521, 935, 971, 1172
MboI GATC 8 cut(s) 103, 154, 477, 486, 519, 933, 969, 1170
MboII GAAGA 6 cut(s) 496, 602, 608, 614, 844, 979
MflI RGATCY 3 cut(s) 477, 969, 1170
MhlI GDGCHC 2 cut(s) 149, 808
MlsI TGGCCA 1 cut(s) 495
MluCI AATT 4 cut(s) 670, 846, 876, 996
MluI ACGCGT 1 cut(s) 384
MluNI TGGCCA 1 cut(s) 495
MlyI GAGTC 2 cut(s) 273, 829
MmeI TCCRAC 3 cut(s) 174, 207, 905
Mox20I TGGCCA 1 cut(s) 495
MroXI GAANNNNTTC 1 cut(s) 824
MscI TGGCCA 1 cut(s) 495
MseI TTAA 2 cut(s) 339, 1163
Msp20I TGGCCA 1 cut(s) 495
MspA1I CMGCKG 1 cut(s) 201
MspI CCGG 4 cut(s) 220, 234, 650, 782
MspR9I CCNGG 3 cut(s) 221, 235, 666
Mva1269I GAATGC 1 cut(s) 1105
MvaI CCWGG 1 cut(s) 666
MvnI CGCG 3 cut(s) 201, 376, 386
MwoI GCNNNNNNNGC 8 cut(s) 70, 76, 79, 186, 195, 204, 316, 622
NciI CCSGG 2 cut(s) 221, 235
NcoI CCATGG 2 cut(s) 496, 866
NdeII GATC 8 cut(s) 103, 154, 477, 486, 519, 933, 969, 1170
NlaIII CATG 3 cut(s) 500, 766, 870
NlaIV GGNNCC 1 cut(s) 843
NmeAIII GCCGAG 1 cut(s) 499
NmuCI GTSAC 4 cut(s) 121, 190, 322, 506
NspV TTCGAA 1 cut(s) 467
PctI GAATGC 1 cut(s) 1105
PdmI GAANNNNTTC 1 cut(s) 824
PfeI GAWTC 4 cut(s) 116, 161, 563, 707
PflFI GACNNNGTC 1 cut(s) 263
PkrI GCNGC 9 cut(s) 66, 69, 72, 81, 188, 200, 378, 381, 663
PleI GAGTC 2 cut(s) 272, 829
PpsI GAGTC 2 cut(s) 272, 829
PshAI GACNNNNGTC 1 cut(s) 213
PshBI ATTAAT 1 cut(s) 339
Psp124BI GAGCTC 2 cut(s) 149, 808
Psp6I CCWGG 1 cut(s) 664
PspGI CCWGG 1 cut(s) 664
PspN4I GGNNCC 1 cut(s) 843
PspPI GGNCC 1 cut(s) 1139
PsuI RGATCY 3 cut(s) 477, 969, 1170
PsyI GACNNNGTC 1 cut(s) 263
RsaI GTAC 2 cut(s) 757, 1112
RsaNI GTAC 2 cut(s) 756, 1111
Rsr2I CGGWCCG 1 cut(s) 1139
RsrII CGGWCCG 1 cut(s) 1139
SacI GAGCTC 2 cut(s) 149, 808
SacII CCGCGG 1 cut(s) 202
SaqAI TTAA 2 cut(s) 339, 1163
SatI GCNGC 9 cut(s) 65, 68, 71, 80, 187, 199, 377, 380, 662
Sau3AI GATC 8 cut(s) 103, 154, 477, 486, 519, 933, 969, 1170
Sau96I GGNCC 1 cut(s) 1139
ScaI AGTACT 1 cut(s) 757
SchI GAGTC 2 cut(s) 273, 829
ScrFI CCNGG 3 cut(s) 221, 235, 666
SduI GDGCHC 2 cut(s) 149, 808
SfaNI GCATC 3 cut(s) 625, 861, 1134
Sfr303I CCGCGG 1 cut(s) 202
SfuI TTCGAA 1 cut(s) 467
SgrBI CCGCGG 1 cut(s) 202
SinI GGWCC 1 cut(s) 1139
SmlI CTYRAG 3 cut(s) 589, 696, 973
SmoI CTYRAG 3 cut(s) 589, 696, 973
Sse9I AATT 4 cut(s) 670, 846, 876, 996
SsiI CCGC 7 cut(s) 68, 71, 80, 199, 201, 376, 1128
SspMI CTAG 1 cut(s) 921
SstI GAGCTC 2 cut(s) 149, 808
StyD4I CCNGG 3 cut(s) 219, 233, 664
StyI CCWWGG 2 cut(s) 496, 866
TaaI ACNGT 6 cut(s) 11, 215, 328, 512, 608, 681
TaiI ACGT 3 cut(s) 25, 137, 324
TaqI TCGA 2 cut(s) 467, 482
TaqII GACCGA 1 cut(s) 1156
TasI AATT 4 cut(s) 670, 846, 876, 996
TatI WGTACW 2 cut(s) 755, 1110
TauI GCSGC 5 cut(s) 70, 73, 82, 201, 379
TfiI GAWTC 4 cut(s) 116, 161, 563, 707
Tru1I TTAA 2 cut(s) 339, 1163
Tru9I TTAA 2 cut(s) 339, 1163
TscAI CASTG 3 cut(s) 16, 515, 898
TseFI GTSAC 4 cut(s) 121, 190, 322, 506
TseI GCWGC 4 cut(s) 64, 186, 379, 661
Tsp45I GTSAC 4 cut(s) 121, 190, 322, 506
TspDTI ATGAA 2 cut(s) 615, 779
TspGWI ACGGA 4 cut(s) 15, 109, 1104, 1152
TspRI CASTG 3 cut(s) 16, 515, 898
Tth111I GACNNNGTC 1 cut(s) 263
VpaK11BI GGWCC 1 cut(s) 1139
VspI ATTAAT 1 cut(s) 339
XagI CCTNNNNNAGG 1 cut(s) 1071
XcmI CCANNNNNNNNNTGG 1 cut(s) 864
XmnI GAANNNNTTC 1 cut(s) 824
XspI CTAG 1 cut(s) 921
ZraI GACGTC 1 cut(s) 135
ZrmI AGTACT 1 cut(s) 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.