MD14G1180300.v1.1
NAC Family

inactive poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007192
Physical Location & Seq
Forward (+)
27298344 .. 27301602
3259 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1180300.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGAGTACAGCAATGATGTTACGAATCTTTCTTCTCGAAATTCATCGGAAGATGAACTCAGCGATCAATTTACAGATTCGGGTACCACCGACTCTGCCTCAGAGACCGTCCAGTCGTCCATCTCAGACTGCGAAAGCGTTGTCGATTCCGGTTCTTCAAGTTCCCGGAATCAATCTCGGCCGTTCGGTGATGGGTTGGTCCCGCTTTTCAACGGCGACAGAGTTCACGACCTCATCAAGCAGCGGTTCGTTTCGAGTTTGGGATTGCTTGGGACGCAAGTCAGTGTTGTGGCGGTTCACAGAAACTCCTACTCGAGCCCTGTGGGCCAAGCAAGGCTGCGTTCGTTTCAAATTTACTTGAAAGCGGTGGAGGAGAAGTGCGGGGGAAATCCCAACGTGAAGTACGCTTGGTACGCGCCCTATTCGAAAGACGAGATTTCGAAGATCATCTGCCATGGTTTCGGCAACTTCGAGCAGCAACAGAGAAATGGGGTGTATGGCAATGGTGTTTATCTTGCTCCCGATCATTCTCCTATGCAATGTGTGGAAGGCTTGAATGTTGACGAAGACGGTTTACGCCATCTTTTGCTCTGCCGTGTCATATTGGGGAGGCCGGAGGTTGTGTGCCCTGGCTCTAAACAGCATCATCCGAGCTCCGAACAGTTTGATTCGGGCATCGACAACATCTTTGCTCCCAAAAAGTACTTAGTTTGGAGTACACACATGAATACTCACATCTTGCCGGAGTATGTCATCAGTTTCAGAGCTCCTAGTTGCTTGAAAGATTTCTTGAAGATTCAAGAACCAATCAAGAAACCGACCTCGCCGTGGATGCCATTTCCGGTGCTAATCGGCGTGCTTTCGAAGTTCTTGCCTCCACCTACCATTGCCTTGATCAGCAAGTATTACAAAGAACACAGAGCGAATGTGATCACAAGGCAACAACTAATACAAAAAGTGAGACAAATTGCAGGAGACGAGTTGCTGGTTTCAATTATCAAATCTTTTAGAGCCAAGCAAATCAAACCACTGCAAAGAGTTGCTCAAAATGGAGGCAGGAGTAGGATGGACGGTGTAGATGCGCCAATTTTCTTTTGA

Protein Analysis

366

Amino Acids

40.69

Weight (kDa)

8.37

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 272 - 337 1.8e-26 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 112
Acc65I GGTACC 1 cut(s) 83
AccB1I GGYRCC 1 cut(s) 83
AccII CGCG 1 cut(s) 416
AciI CCGC 5 cut(s) 203, 244, 293, 365, 381
AcoI YGGCCR 1 cut(s) 179
AcsI RAATTY 2 cut(s) 40, 351
AfaI GTAC 6 cut(s) 8, 85, 404, 413, 704, 718
AfiI CCNNNNNNNGG 1 cut(s) 828
AgsI TTSAA 9 cut(s) 159, 211, 350, 361, 556, 781, 793, 800, 993
AjnI CCWGG 1 cut(s) 628
AluBI AGCT 2 cut(s) 654, 767
AluI AGCT 2 cut(s) 654, 767
Alw21I GWGCWC 2 cut(s) 656, 769
Alw26I GTCTC 3 cut(s) 98, 955, 969
Ama87I CYCGRG 1 cut(s) 313
AoxI GGCC 3 cut(s) 179, 325, 611
ApeKI GCWGC 3 cut(s) 241, 337, 475
ApoI RAATTY 2 cut(s) 40, 351
ArsI GACNNNNNNTTYG 4 cut(s) 126, 158, 671, 703
Asp700I GAANNNNTTC 1 cut(s) 785
Asp718I GGTACC 1 cut(s) 83
AspLEI GCGC 2 cut(s) 418, 1084
AspS9I GGNCC 2 cut(s) 199, 325
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 200
AsuII TTCGAA 3 cut(s) 425, 440, 863
AvaI CYCGRG 1 cut(s) 313
AvaII GGWCC 1 cut(s) 199
BaeGI GKGCMC 1 cut(s) 629
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 3 cut(s) 320, 656, 769
BarI GAAGNNNNNNTAC 2 cut(s) 558, 590
BbsI GAAGAC 1 cut(s) 573
Bbv12I GWGCWC 2 cut(s) 656, 769
BbvI GCAGC 3 cut(s) 253, 324, 487
BccI CCATC 4 cut(s) 128, 185, 588, 1060
BceAI ACGGC 4 cut(s) 166, 229, 579, 811
BcgI CGANNNNNNTGC 2 cut(s) 853, 887
BciT130I CCWGG 1 cut(s) 630
BclI TGATCA 2 cut(s) 894, 930
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 3 cut(s) 98, 955, 969
BfaI CTAG 1 cut(s) 771
BglI GCCNNNNNGGC 1 cut(s) 324
BisI GCNGC 3 cut(s) 242, 338, 476
BlsI GCNGC 3 cut(s) 243, 339, 477
BmcAI AGTACT 1 cut(s) 704
Bme1390I CCNGG 2 cut(s) 166, 630
Bme18I GGWCC 1 cut(s) 199
BmeT110I CYCGRG 1 cut(s) 313
BmgT120I GGNCC 2 cut(s) 199, 325
BmiI GGNNCC 2 cut(s) 85, 201
BmrFI CCNGG 2 cut(s) 166, 630
BmsI GCATC 4 cut(s) 652, 684, 822, 1069
BoxI GACNNNNGTC 1 cut(s) 278
BpiI GAAGAC 1 cut(s) 573
Bpu14I TTCGAA 3 cut(s) 425, 440, 863
BpuMI CCSGG 1 cut(s) 166
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 3 cut(s) 454, 628, 827
BsaWI WCCGGW 2 cut(s) 149, 841
BsaXI ACNNNNNCTCC 6 cut(s) 290, 320, 362, 392, 608, 638
Bsc4I CCNNNNNNNGG 1 cut(s) 828
Bse1I ACTGG 1 cut(s) 112
Bse3DI GCAATG 4 cut(s) 19, 508, 545, 885
BseBI CCWGG 1 cut(s) 630
BseDI CCNNGG 3 cut(s) 454, 628, 827
BseGI GGATG 3 cut(s) 646, 837, 1071
BseLI CCNNNNNNNGG 1 cut(s) 828
BseMI GCAATG 4 cut(s) 19, 508, 545, 885
BseMII CTCAG 3 cut(s) 73, 114, 138
BseNI ACTGG 1 cut(s) 112
BseRI GAGGAG 1 cut(s) 386
BseSI GKGCMC 1 cut(s) 629
BseX3I CGGCCG 1 cut(s) 179
BseXI GCAGC 3 cut(s) 253, 324, 487
Bsh1236I CGCG 1 cut(s) 416
Bsh1285I CGRYCG 1 cut(s) 182
BshFI GGCC 3 cut(s) 181, 327, 613
BshNI GGYRCC 1 cut(s) 83
BsiEI CGRYCG 1 cut(s) 182
BsiHKAI GWGCWC 2 cut(s) 656, 769
BsiHKCI CYCGRG 1 cut(s) 313
BsiSI CCGG 5 cut(s) 150, 166, 614, 743, 842
BslFI GGGAC 2 cut(s) 185, 286
BslI CCNNNNNNNGG 1 cut(s) 828
BsmAI GTCTC 3 cut(s) 98, 955, 969
BsmBI CGTCTC 1 cut(s) 969
BsmFI GGGAC 2 cut(s) 185, 286
BsnI GGCC 3 cut(s) 181, 327, 613
Bso31I GGTCTC 1 cut(s) 98
BsoBI CYCGRG 1 cut(s) 313
Bsp119I TTCGAA 3 cut(s) 425, 440, 863
Bsp1286I GDGCHC 4 cut(s) 320, 629, 656, 769
Bsp143I GATC 5 cut(s) 64, 444, 523, 894, 930
Bsp19I CCATGG 1 cut(s) 454
BspACI CCGC 5 cut(s) 203, 244, 293, 365, 381
BspANI GGCC 3 cut(s) 181, 327, 613
BspCNI CTCAG 3 cut(s) 72, 113, 137
BspFNI CGCG 1 cut(s) 416
BspLI GGNNCC 2 cut(s) 85, 201
BspT104I TTCGAA 3 cut(s) 425, 440, 863
BspT107I GGYRCC 1 cut(s) 83
BspTNI GGTCTC 1 cut(s) 98
BsrDI GCAATG 4 cut(s) 19, 508, 545, 885
BsrI ACTGG 1 cut(s) 112
BssECI CCNNGG 3 cut(s) 454, 628, 827
BssMI GATC 5 cut(s) 64, 444, 523, 894, 930
BssT1I CCWWGG 1 cut(s) 454
Bst2UI CCWGG 1 cut(s) 630
Bst4CI ACNGT 4 cut(s) 109, 572, 663, 1073
BstBI TTCGAA 3 cut(s) 425, 440, 863
BstC8I GCNNGC 1 cut(s) 857
BstDEI CTNAG 4 cut(s) 59, 100, 124, 706
BstDSI CCRYGG 2 cut(s) 454, 827
BstF5I GGATG 3 cut(s) 646, 837, 1071
BstFNI CGCG 1 cut(s) 416
BstHHI GCGC 2 cut(s) 418, 1084
BstKTI GATC 5 cut(s) 67, 447, 526, 897, 933
BstMAI GTCTC 3 cut(s) 98, 955, 969
BstMBI GATC 5 cut(s) 64, 444, 523, 894, 930
BstMCI CGRYCG 1 cut(s) 182
BstMWI GCNNNNNNNGC 4 cut(s) 274, 324, 413, 832
BstNI CCWGG 1 cut(s) 630
BstPAI GACNNNNGTC 1 cut(s) 278
BstSCI CCNGG 2 cut(s) 164, 628
BstSLI GKGCMC 1 cut(s) 629
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 3 cut(s) 253, 324, 487
BstV2I GAAGAC 1 cut(s) 573
BstZI CGGCCG 1 cut(s) 179
BsuRI GGCC 3 cut(s) 181, 327, 613
BtgI CCRYGG 2 cut(s) 454, 827
BtsCI GGATG 3 cut(s) 646, 837, 1071
BtsI GCAGTG 1 cut(s) 1028
BtsIMutI CAGTG 2 cut(s) 289, 1028
Cac8I GCNNGC 1 cut(s) 857
CfoI GCGC 2 cut(s) 418, 1084
Cfr13I GGNCC 2 cut(s) 199, 325
CseI GACGC 1 cut(s) 283
Csp6I GTAC 6 cut(s) 7, 84, 403, 412, 703, 717
CviAII CATG 2 cut(s) 455, 724
CviQI GTAC 6 cut(s) 7, 84, 403, 412, 703, 717
DdeI CTNAG 4 cut(s) 59, 100, 124, 706
DpnI GATC 5 cut(s) 66, 446, 525, 896, 932
DpnII GATC 5 cut(s) 64, 444, 523, 894, 930
DrdI GACNNNNNNGTC 1 cut(s) 112
DseDI GACNNNNNNGTC 1 cut(s) 112
EaeI YGGCCR 1 cut(s) 179
EagI CGGCCG 1 cut(s) 179
Ecl136II GAGCTC 2 cut(s) 654, 767
EclXI CGGCCG 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 454
Eco24I GRGCYC 3 cut(s) 320, 656, 769
Eco31I GGTCTC 1 cut(s) 98
Eco47I GGWCC 1 cut(s) 199
Eco52I CGGCCG 1 cut(s) 179
Eco53kI GAGCTC 2 cut(s) 654, 767
Eco88I CYCGRG 1 cut(s) 313
EcoICRI GAGCTC 2 cut(s) 654, 767
EcoRII CCWGG 1 cut(s) 628
EcoT14I CCWWGG 1 cut(s) 454
EcoT38I GRGCYC 3 cut(s) 320, 656, 769
ErhI CCWWGG 1 cut(s) 454
Esp3I CGTCTC 1 cut(s) 969
FaeI CATG 2 cut(s) 458, 727
FaiI YATR 6 cut(s) 456, 498, 536, 602, 725, 750
FaqI GGGAC 2 cut(s) 185, 286
FatI CATG 2 cut(s) 454, 723
FauI CCCGC 2 cut(s) 210, 374
FbaI TGATCA 2 cut(s) 894, 930
Fnu4HI GCNGC 3 cut(s) 242, 338, 476
FokI GGATG 3 cut(s) 633, 844, 1078
FriOI GRGCYC 3 cut(s) 320, 656, 769
Fsp4HI GCNGC 3 cut(s) 242, 338, 476
FspBI CTAG 1 cut(s) 771
GlaI GCGC 2 cut(s) 417, 1083
GluI GCNGC 3 cut(s) 242, 338, 476
HaeIII GGCC 3 cut(s) 181, 327, 613
HapII CCGG 5 cut(s) 150, 166, 614, 743, 842
HgaI GACGC 1 cut(s) 283
HhaI GCGC 2 cut(s) 418, 1084
Hin1II CATG 2 cut(s) 458, 727
Hin6I GCGC 2 cut(s) 416, 1082
HinP1I GCGC 2 cut(s) 416, 1082
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HinfI GANTC 7 cut(s) 25, 77, 92, 146, 169, 668, 796
HpaII CCGG 5 cut(s) 150, 166, 614, 743, 842
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 5 cut(s) 226, 298, 562, 575, 719
Hpy188I TCNGA 6 cut(s) 49, 103, 127, 651, 658, 764
Hpy188III TCNNGA 6 cut(s) 36, 227, 521, 790, 800, 811
Hpy8I GTNNAC 5 cut(s) 226, 298, 562, 575, 719
HpyAV CCTTC 1 cut(s) 542
HpyCH4III ACNGT 4 cut(s) 109, 572, 663, 1073
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 3 cut(s) 538, 971, 1033
HpyF10VI GCNNNNNNNGC 4 cut(s) 274, 324, 413, 832
HpyF3I CTNAG 4 cut(s) 59, 100, 124, 706
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 2 cut(s) 458, 727
HspAI GCGC 2 cut(s) 416, 1082
KpnI GGTACC 1 cut(s) 87
Ksp22I TGATCA 2 cut(s) 894, 930
Kzo9I GATC 5 cut(s) 64, 444, 523, 894, 930
LmnI GCTCC 4 cut(s) 523, 659, 697, 772
Lsp1109I GCAGC 3 cut(s) 253, 324, 487
LweI GCATC 4 cut(s) 652, 684, 822, 1069
MaeI CTAG 1 cut(s) 771
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 1 cut(s) 19
MalI GATC 5 cut(s) 66, 446, 525, 896, 932
MboI GATC 5 cut(s) 64, 444, 523, 894, 930
MboII GAAGA 6 cut(s) 24, 62, 147, 454, 578, 805
MhlI GDGCHC 4 cut(s) 320, 629, 656, 769
MluCI AATT 6 cut(s) 40, 68, 351, 966, 993, 1086
MlyI GAGTC 1 cut(s) 86
MnlI CCTC 8 cut(s) 109, 242, 364, 603, 610, 832, 885, 1046
MroXI GAANNNNTTC 1 cut(s) 785
MspA1I CMGCKG 1 cut(s) 244
MspI CCGG 5 cut(s) 150, 166, 614, 743, 842
MspR9I CCNGG 2 cut(s) 166, 630
MvaI CCWGG 1 cut(s) 630
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 4 cut(s) 274, 324, 413, 832
NciI CCSGG 1 cut(s) 166
NcoI CCATGG 1 cut(s) 454
NdeII GATC 5 cut(s) 64, 444, 523, 894, 930
NlaIII CATG 2 cut(s) 458, 727
NlaIV GGNNCC 2 cut(s) 85, 201
NmeAIII GCCGAG 1 cut(s) 157
NspV TTCGAA 3 cut(s) 425, 440, 863
PaeR7I CTCGAG 1 cut(s) 313
PcsI WCGNNNNNNNCGW 1 cut(s) 468
PdmI GAANNNNTTC 1 cut(s) 785
PfeI GAWTC 6 cut(s) 25, 77, 146, 169, 668, 796
PfoI TCCNGGA 1 cut(s) 164
PkrI GCNGC 3 cut(s) 243, 339, 477
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
PshAI GACNNNNGTC 1 cut(s) 278
Psp124BI GAGCTC 2 cut(s) 656, 769
Psp6I CCWGG 1 cut(s) 628
PspGI CCWGG 1 cut(s) 628
PspN4I GGNNCC 2 cut(s) 85, 201
PspPI GGNCC 2 cut(s) 199, 325
PspXI VCTCGAGB 1 cut(s) 313
RsaI GTAC 6 cut(s) 8, 85, 404, 413, 704, 718
RsaNI GTAC 6 cut(s) 7, 84, 403, 412, 703, 717
SacI GAGCTC 2 cut(s) 656, 769
SatI GCNGC 3 cut(s) 242, 338, 476
Sau3AI GATC 5 cut(s) 64, 444, 523, 894, 930
Sau96I GGNCC 2 cut(s) 199, 325
ScaI AGTACT 1 cut(s) 704
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 2 cut(s) 166, 630
SduI GDGCHC 4 cut(s) 320, 629, 656, 769
SetI ASST 7 cut(s) 234, 399, 621, 656, 769, 824, 883
SfaNI GCATC 4 cut(s) 652, 684, 822, 1069
Sfr274I CTCGAG 1 cut(s) 313
SfuI TTCGAA 3 cut(s) 425, 440, 863
SinI GGWCC 1 cut(s) 199
SlaI CTCGAG 1 cut(s) 313
SmlI CTYRAG 1 cut(s) 313
SmoI CTYRAG 1 cut(s) 313
Sse9I AATT 6 cut(s) 40, 68, 351, 966, 993, 1086
SsiI CCGC 5 cut(s) 203, 244, 293, 365, 381
SspMI CTAG 1 cut(s) 771
SstI GAGCTC 2 cut(s) 656, 769
StyD4I CCNGG 2 cut(s) 164, 628
StyI CCWWGG 1 cut(s) 454
TaaI ACNGT 4 cut(s) 109, 572, 663, 1073
TaiI ACGT 1 cut(s) 399
TaqI TCGA 9 cut(s) 37, 144, 254, 314, 425, 440, 471, 678, 863
TasI AATT 6 cut(s) 40, 68, 351, 966, 993, 1086
TatI WGTACW 3 cut(s) 6, 702, 716
TfiI GAWTC 6 cut(s) 25, 77, 146, 169, 668, 796
TscAI CASTG 2 cut(s) 289, 1035
TseI GCWGC 3 cut(s) 241, 337, 475
TspDTI ATGAA 3 cut(s) 33, 69, 740
TspRI CASTG 2 cut(s) 289, 1035
VpaK11BI GGWCC 1 cut(s) 199
XapI RAATTY 2 cut(s) 40, 351
XhoI CTCGAG 1 cut(s) 313
XmnI GAANNNNTTC 1 cut(s) 785
XspI CTAG 1 cut(s) 771
ZrmI AGTACT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.