RLG00000004264
NAC Family

inactive poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007192
Physical Location & Seq
Reverse (-)
57811861 .. 57813872
2012 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004264

Sequence Viewer

Length: 1164 bp
ATGGAATACAGCGGTGTTCAACGTCCGTTATCCTCTGTTTTCGCCGATGGGTTCACAGCCTCGCCGCCGCCGCCTAACGATTGCGAACTCTCCGATCAGGTTTCCGATTCCGTCACCACAGAGACGTCCTCAGGGAGCTCCGACGATCAAGAATCATCGGTCTCCGATTGCGAAAGCAGCGTCACGAGCCGCGGCGACGACCGTCGCGGGGTGTTGGACGCCGGGTTAGTGAAACTTGGAGAAGGGGACAGAGTCCACGACCTCATCAAGCAAAGGTTTCTTGCGAGTTTGGGATTGCTTGGAGTGCACGTCACCGTTACGGCGATTAATAGAAACTCCCATTCGGGTTTTACGGGTCAAGCGCGGCTGCACGCGTTTCGGGTTTACTTGAAAGCTGTGGAGGACAAGTGTGGTGGAAACGCCAATGTGAAATATGGTTGGTATTCTCCGAGTTCGAAAGCCGAGATTTCGAAGATCATTGGCCATGGATTTGGTCACTGTGAAAAGATCAACGGGGTTTATGGTAGAGGTGTCTATCTTGCTCCTGATGATTCACCTTTGGAAAGTTTGGAAAACTTGAGTGAAGATGAAGACGGTCTTCGGCATCTTTTGCTTTGCCGTGTGATTTTGGGGAAGCAGGAAGTTGTGCTGCCTGGTAATTCTGAACTGTATCATCCCAGTTCGCAAGAGTTTGATTCGGGTGTGGACAGGCTTTTAGCTCCCAAGAAGTATATAGTGTGGAGTACTCACATGAACACCCATATCTTGCCGGAGTATGTCATCAGCTTCAGAGCTCCTACTTGCCTGAAAGAGTTCTTGAAGACTCGGGAACCAATTAGAAAACCAACTTCACCATGGATGCCAATTCCAACTCTAATCAGTGTGCTTTCGAAGTTCTTGCCTCAGCCTAGCATTGCTTTGATCATCAAGTATCATAAAGCCCACAGAGAAACGAAGATCTCAAGGAGTGAACTAATTCAGAGAATTAGACAAATAGCAGGAGACAAGTTGCTGGCTACAATCATCAAATCTTTCAGAACCAAGCAACTCAAATCTTTGCAAGGGGTTTCTCAGGACGGAGGCAGGAATGCGATGGAGTACAAAAATAATGCAGGCGGTTTAGACGGACCTATGCTTTTTGGAGTAAACTTAAAACAGATCTAA

Protein Analysis

388

Amino Acids

42.57

Weight (kDa)

8.12

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 281 - 346 2.8e-24 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 128
AccII CGCG 4 cut(s) 192, 207, 364, 374
AciI CCGC 9 cut(s) 12, 65, 68, 71, 190, 192, 207, 364, 1116
AcoI YGGCCR 1 cut(s) 481
AcuI CTGAAG 1 cut(s) 772
AcyI GRCGYC 2 cut(s) 125, 219
AfaI GTAC 2 cut(s) 745, 1100
AfiI CCNNNNNNNGG 1 cut(s) 208
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 3 cut(s) 20, 391, 820
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 1 cut(s) 652
AluBI AGCT 5 cut(s) 138, 395, 719, 786, 794
AluI AGCT 5 cut(s) 138, 395, 719, 786, 794
Alw21I GWGCWC 3 cut(s) 140, 309, 796
Alw26I GTCTC 3 cut(s) 116, 166, 996
Alw44I GTGCAC 1 cut(s) 305
Ama87I CYCGRG 1 cut(s) 825
AoxI GGCC 1 cut(s) 481
ApaLI GTGCAC 1 cut(s) 305
ApeKI GCWGC 3 cut(s) 177, 367, 649
ArsI GACNNNNNNTTYG 2 cut(s) 165, 197
AseI ATTAAT 1 cut(s) 327
Asp700I GAANNNNTTC 2 cut(s) 812, 975
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 1 cut(s) 1127
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 4 cut(s) 106, 304, 546, 843
AsuII TTCGAA 3 cut(s) 455, 470, 890
AvaI CYCGRG 1 cut(s) 825
AvaII GGWCC 1 cut(s) 1127
AxyI CCTNAGG 1 cut(s) 130
BaeGI GKGCMC 1 cut(s) 309
BalI TGGCCA 1 cut(s) 483
BanII GRGCYC 2 cut(s) 140, 796
BauI CACGAG 1 cut(s) 184
BbsI GAAGAC 3 cut(s) 590, 597, 827
Bbv12I GWGCWC 3 cut(s) 140, 309, 796
BbvCI CCTCAGC 1 cut(s) 903
BbvI GCAGC 3 cut(s) 189, 354, 636
BccI CCATC 2 cut(s) 41, 1087
BceAI ACGGC 2 cut(s) 336, 603
BcgI CGANNNNNNTGC 4 cut(s) 359, 393, 880, 914
BciT130I CCWGG 1 cut(s) 654
BclI TGATCA 1 cut(s) 921
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 3 cut(s) 116, 166, 996
BfaI CTAG 1 cut(s) 909
BglII AGATCT 2 cut(s) 957, 1158
BisI GCNGC 9 cut(s) 65, 68, 71, 178, 190, 193, 365, 368, 650
BlsI GCNGC 9 cut(s) 66, 69, 72, 179, 191, 194, 366, 369, 651
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 2 cut(s) 223, 654
Bme18I GGWCC 1 cut(s) 1127
BmeT110I CYCGRG 1 cut(s) 825
BmgBI CACGTC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 1127
BmiI GGNNCC 1 cut(s) 831
BmrFI CCNGG 2 cut(s) 223, 654
BmrI ACTGGG 1 cut(s) 672
BmsI GCATC 2 cut(s) 613, 849
BmuI ACTGGG 1 cut(s) 672
BoxI GACNNNNGTC 1 cut(s) 201
BpiI GAAGAC 3 cut(s) 590, 597, 827
BplI GAGNNNNNCTC 2 cut(s) 113, 145
Bpu10I CCTNAGC 1 cut(s) 903
Bpu14I TTCGAA 3 cut(s) 455, 470, 890
BpuEI CTTGAG 2 cut(s) 598, 946
BpuMI CCSGG 1 cut(s) 223
BsaHI GRCGYC 2 cut(s) 125, 219
BsaI GGTCTC 1 cut(s) 166
BsaJI CCNNGG 3 cut(s) 190, 484, 854
BsaXI ACNNNNNCTCC 4 cut(s) 294, 324, 392, 422
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 678
Bse21I CCTNAGG 1 cut(s) 130
Bse3DI GCAATG 1 cut(s) 912
BseBI CCWGG 1 cut(s) 654
BseDI CCNNGG 3 cut(s) 190, 484, 854
BseGI GGATG 2 cut(s) 673, 864
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 3 cut(s) 144, 917, 1085
BseNI ACTGG 1 cut(s) 678
BseSI GKGCMC 1 cut(s) 309
BseXI GCAGC 3 cut(s) 189, 354, 636
BsgI GTGCAG 1 cut(s) 353
Bsh1236I CGCG 4 cut(s) 192, 207, 364, 374
Bsh1285I CGRYCG 1 cut(s) 202
BshFI GGCC 1 cut(s) 483
BsiEI CGRYCG 1 cut(s) 202
BsiHKAI GWGCWC 3 cut(s) 140, 309, 796
BsiHKCI CYCGRG 1 cut(s) 825
BsiSI CCGG 2 cut(s) 222, 770
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 3 cut(s) 116, 166, 996
BsmBI CGTCTC 1 cut(s) 116
BsmFI GGGAC 1 cut(s) 260
BsmI GAATGC 1 cut(s) 1093
BsnI GGCC 1 cut(s) 483
Bso31I GGTCTC 1 cut(s) 166
BsoBI CYCGRG 1 cut(s) 825
Bsp119I TTCGAA 3 cut(s) 455, 470, 890
Bsp1286I GDGCHC 3 cut(s) 140, 309, 796
Bsp143I GATC 7 cut(s) 94, 145, 474, 507, 921, 957, 1158
Bsp19I CCATGG 2 cut(s) 484, 854
BspACI CCGC 9 cut(s) 12, 65, 68, 71, 190, 192, 207, 364, 1116
BspANI GGCC 1 cut(s) 483
BspCNI CTCAG 3 cut(s) 143, 916, 1084
BspFNI CGCG 4 cut(s) 192, 207, 364, 374
BspLI GGNNCC 1 cut(s) 831
BspT104I TTCGAA 3 cut(s) 455, 470, 890
BspTNI GGTCTC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 912
BsrI ACTGG 1 cut(s) 678
BssECI CCNNGG 3 cut(s) 190, 484, 854
BssMI GATC 7 cut(s) 94, 145, 474, 507, 921, 957, 1158
BssNI GRCGYC 2 cut(s) 125, 219
BssSI CACGAG 1 cut(s) 184
BssT1I CCWWGG 2 cut(s) 484, 854
Bst2BI CACGAG 1 cut(s) 184
Bst2UI CCWGG 1 cut(s) 654
Bst4CI ACNGT 5 cut(s) 203, 316, 500, 596, 669
BstACI GRCGYC 2 cut(s) 125, 219
BstAPI GCANNNNNTGC 1 cut(s) 610
BstBI TTCGAA 3 cut(s) 455, 470, 890
BstC8I GCNNGC 3 cut(s) 372, 1014, 1114
BstDEI CTNAG 3 cut(s) 130, 903, 1071
BstDSI CCRYGG 3 cut(s) 190, 484, 854
BstF5I GGATG 2 cut(s) 673, 864
BstFNI CGCG 4 cut(s) 192, 207, 364, 374
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 7 cut(s) 97, 148, 477, 510, 924, 960, 1161
BstMAI GTCTC 3 cut(s) 116, 166, 996
BstMBI GATC 7 cut(s) 94, 145, 474, 507, 921, 957, 1158
BstMCI CGRYCG 1 cut(s) 202
BstMWI GCNNNNNNNGC 5 cut(s) 70, 177, 186, 304, 610
BstNI CCWGG 1 cut(s) 654
BstPAI GACNNNNGTC 1 cut(s) 201
BstSCI CCNGG 2 cut(s) 221, 652
BstSLI GKGCMC 1 cut(s) 309
BstUI CGCG 4 cut(s) 192, 207, 364, 374
BstV1I GCAGC 3 cut(s) 189, 354, 636
BstV2I GAAGAC 3 cut(s) 590, 597, 827
BstX2I RGATCY 2 cut(s) 957, 1158
BstXI CCANNNNNNTGG 1 cut(s) 491
BstYI RGATCY 2 cut(s) 957, 1158
Bsu36I CCTNAGG 1 cut(s) 130
BsuRI GGCC 1 cut(s) 483
BtgI CCRYGG 3 cut(s) 190, 484, 854
BtgZI GCGATG 1 cut(s) 1106
BtrI CACGTC 1 cut(s) 310
BtsCI GGATG 2 cut(s) 673, 864
BtsIMutI CAGTG 2 cut(s) 496, 886
Cac8I GCNNGC 3 cut(s) 372, 1014, 1114
CfoI GCGC 1 cut(s) 364
Cfr13I GGNCC 1 cut(s) 1127
Cfr42I CCGCGG 1 cut(s) 193
CseI GACGC 2 cut(s) 169, 227
Csp6I GTAC 2 cut(s) 744, 1099
CspCI CAANNNNNGTGG 2 cut(s) 394, 429
CviAII CATG 3 cut(s) 485, 751, 855
CviQI GTAC 2 cut(s) 744, 1099
DdeI CTNAG 3 cut(s) 130, 903, 1071
DpnI GATC 7 cut(s) 96, 147, 476, 509, 923, 959, 1160
DpnII GATC 7 cut(s) 94, 145, 474, 507, 921, 957, 1158
EaeI YGGCCR 1 cut(s) 481
Ecl136II GAGCTC 2 cut(s) 138, 794
Eco130I CCWWGG 2 cut(s) 484, 854
Eco24I GRGCYC 2 cut(s) 140, 796
Eco31I GGTCTC 1 cut(s) 166
Eco47I GGWCC 1 cut(s) 1127
Eco53kI GAGCTC 2 cut(s) 138, 794
Eco57I CTGAAG 1 cut(s) 772
Eco81I CCTNAGG 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 825
EcoICRI GAGCTC 2 cut(s) 138, 794
EcoRII CCWGG 1 cut(s) 652
EcoT14I CCWWGG 2 cut(s) 484, 854
EcoT38I GRGCYC 2 cut(s) 140, 796
ErhI CCWWGG 2 cut(s) 484, 854
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 3 cut(s) 488, 754, 858
FalI AAGNNNNNCTT 2 cut(s) 582, 614
FaqI GGGAC 1 cut(s) 260
FatI CATG 3 cut(s) 484, 750, 854
FauI CCCGC 1 cut(s) 200
FbaI TGATCA 1 cut(s) 921
Fnu4HI GCNGC 9 cut(s) 65, 68, 71, 178, 190, 193, 365, 368, 650
FokI GGATG 2 cut(s) 660, 871
FriOI GRGCYC 2 cut(s) 140, 796
Fsp4HI GCNGC 9 cut(s) 65, 68, 71, 178, 190, 193, 365, 368, 650
FspBI CTAG 1 cut(s) 909
GlaI GCGC 1 cut(s) 363
GluI GCNGC 9 cut(s) 65, 68, 71, 178, 190, 193, 365, 368, 650
HaeIII GGCC 1 cut(s) 483
HapII CCGG 2 cut(s) 222, 770
HgaI GACGC 2 cut(s) 169, 227
HhaI GCGC 1 cut(s) 364
Hin1I GRCGYC 2 cut(s) 125, 219
Hin1II CATG 3 cut(s) 488, 754, 858
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HinfI GANTC 6 cut(s) 107, 152, 252, 551, 695, 823
HpaII CCGG 2 cut(s) 222, 770
HphI GGTGA 4 cut(s) 106, 304, 546, 843
Hpy166II GTNNAC 7 cut(s) 54, 256, 307, 385, 706, 971, 1147
Hpy188I TCNGA 9 cut(s) 94, 106, 142, 166, 450, 664, 791, 981, 1037
Hpy188III TCNNGA 6 cut(s) 149, 184, 545, 817, 827, 1073
Hpy8I GTNNAC 7 cut(s) 54, 256, 307, 385, 706, 971, 1147
Hpy99I CGWCG 3 cut(s) 146, 200, 207
HpyAV CCTTC 1 cut(s) 236
HpyCH4III ACNGT 5 cut(s) 203, 316, 500, 596, 669
HpyCH4IV ACGT 3 cut(s) 22, 125, 309
HpyCH4V TGCA 4 cut(s) 307, 370, 1060, 1112
HpyF10VI GCNNNNNNNGC 5 cut(s) 70, 177, 186, 304, 610
HpyF3I CTNAG 3 cut(s) 130, 903, 1071
HpySE526I ACGT 3 cut(s) 22, 125, 309
Hsp92I GRCGYC 2 cut(s) 125, 219
Hsp92II CATG 3 cut(s) 488, 754, 858
HspAI GCGC 1 cut(s) 362
Ksp22I TGATCA 1 cut(s) 921
KspI CCGCGG 1 cut(s) 193
Kzo9I GATC 7 cut(s) 94, 145, 474, 507, 921, 957, 1158
LmnI GCTCC 5 cut(s) 135, 143, 547, 724, 799
Lsp1109I GCAGC 3 cut(s) 189, 354, 636
LweI GCATC 2 cut(s) 613, 849
MaeI CTAG 1 cut(s) 909
MaeII ACGT 3 cut(s) 22, 125, 309
MaeIII GTNAC 5 cut(s) 112, 181, 310, 316, 494
MalI GATC 7 cut(s) 96, 147, 476, 509, 923, 959, 1160
MboI GATC 7 cut(s) 94, 145, 474, 507, 921, 957, 1158
MboII GAAGA 6 cut(s) 484, 590, 596, 602, 832, 967
MflI RGATCY 2 cut(s) 957, 1158
MhlI GDGCHC 3 cut(s) 140, 309, 796
MlsI TGGCCA 1 cut(s) 483
MluCI AATT 5 cut(s) 658, 834, 864, 975, 984
MluI ACGCGT 1 cut(s) 372
MluNI TGGCCA 1 cut(s) 483
MlyI GAGTC 2 cut(s) 261, 817
MmeI TCCRAC 3 cut(s) 165, 195, 893
MnlI CCTC 8 cut(s) 43, 70, 139, 272, 394, 521, 912, 1073
Mox20I TGGCCA 1 cut(s) 483
MroXI GAANNNNTTC 2 cut(s) 812, 975
MscI TGGCCA 1 cut(s) 483
MseI TTAA 2 cut(s) 327, 1151
Msp20I TGGCCA 1 cut(s) 483
MspA1I CMGCKG 2 cut(s) 12, 192
MspI CCGG 2 cut(s) 222, 770
MspR9I CCNGG 2 cut(s) 223, 654
Mva1269I GAATGC 1 cut(s) 1093
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 4 cut(s) 192, 207, 364, 374
MwoI GCNNNNNNNGC 5 cut(s) 70, 177, 186, 304, 610
NciI CCSGG 1 cut(s) 223
NcoI CCATGG 2 cut(s) 484, 854
NdeII GATC 7 cut(s) 94, 145, 474, 507, 921, 957, 1158
NlaIII CATG 3 cut(s) 488, 754, 858
NlaIV GGNNCC 1 cut(s) 831
NmeAIII GCCGAG 1 cut(s) 487
NmuCI GTSAC 4 cut(s) 112, 181, 310, 494
NspV TTCGAA 3 cut(s) 455, 470, 890
PctI GAATGC 1 cut(s) 1093
PdmI GAANNNNTTC 2 cut(s) 812, 975
PfeI GAWTC 4 cut(s) 107, 152, 551, 695
PflFI GACNNNGTC 1 cut(s) 251
PkrI GCNGC 9 cut(s) 66, 69, 72, 179, 191, 194, 366, 369, 651
PleI GAGTC 2 cut(s) 260, 817
PpsI GAGTC 2 cut(s) 260, 817
PshAI GACNNNNGTC 1 cut(s) 201
PshBI ATTAAT 1 cut(s) 327
Psp124BI GAGCTC 2 cut(s) 140, 796
Psp6I CCWGG 1 cut(s) 652
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 1 cut(s) 831
PspPI GGNCC 1 cut(s) 1127
PsuI RGATCY 2 cut(s) 957, 1158
PsyI GACNNNGTC 1 cut(s) 251
RsaI GTAC 2 cut(s) 745, 1100
RsaNI GTAC 2 cut(s) 744, 1099
SacI GAGCTC 2 cut(s) 140, 796
SacII CCGCGG 1 cut(s) 193
SaqAI TTAA 2 cut(s) 327, 1151
SatI GCNGC 9 cut(s) 65, 68, 71, 178, 190, 193, 365, 368, 650
Sau3AI GATC 7 cut(s) 94, 145, 474, 507, 921, 957, 1158
Sau96I GGNCC 1 cut(s) 1127
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 2 cut(s) 261, 817
ScrFI CCNGG 2 cut(s) 223, 654
SduI GDGCHC 3 cut(s) 140, 309, 796
SfaNI GCATC 2 cut(s) 613, 849
Sfr303I CCGCGG 1 cut(s) 193
SfuI TTCGAA 3 cut(s) 455, 470, 890
SgrBI CCGCGG 1 cut(s) 193
SinI GGWCC 1 cut(s) 1127
SmlI CTYRAG 2 cut(s) 577, 961
SmoI CTYRAG 2 cut(s) 577, 961
Sse9I AATT 5 cut(s) 658, 834, 864, 975, 984
SsiI CCGC 9 cut(s) 12, 65, 68, 71, 190, 192, 207, 364, 1116
SspMI CTAG 1 cut(s) 909
SstI GAGCTC 2 cut(s) 140, 796
StyD4I CCNGG 2 cut(s) 221, 652
StyI CCWWGG 2 cut(s) 484, 854
TaaI ACNGT 5 cut(s) 203, 316, 500, 596, 669
TaiI ACGT 3 cut(s) 25, 128, 312
TaqI TCGA 3 cut(s) 455, 470, 890
TaqII GACCGA 1 cut(s) 148
TasI AATT 5 cut(s) 658, 834, 864, 975, 984
TatI WGTACW 2 cut(s) 743, 1098
TauI GCSGC 6 cut(s) 67, 70, 73, 192, 195, 367
TfiI GAWTC 4 cut(s) 107, 152, 551, 695
Tru1I TTAA 2 cut(s) 327, 1151
Tru9I TTAA 2 cut(s) 327, 1151
TscAI CASTG 2 cut(s) 503, 886
TseFI GTSAC 4 cut(s) 112, 181, 310, 494
TseI GCWGC 3 cut(s) 177, 367, 649
Tsp45I GTSAC 4 cut(s) 112, 181, 310, 494
TspDTI ATGAA 2 cut(s) 603, 767
TspGWI ACGGA 4 cut(s) 15, 100, 1092, 1140
TspRI CASTG 2 cut(s) 503, 886
Tth111I GACNNNGTC 1 cut(s) 251
VneI GTGCAC 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 1127
VspI ATTAAT 1 cut(s) 327
XcmI CCANNNNNNNNNTGG 1 cut(s) 852
XmnI GAANNNNTTC 2 cut(s) 812, 975
XspI CTAG 1 cut(s) 909
ZraI GACGTC 1 cut(s) 126
ZrmI AGTACT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.