MD06G1173800.v1.1
NAC Family

inactive poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007192
Physical Location & Seq
Forward (+)
31393747 .. 31396730
2984 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1173800.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGGAGTACAGAAATGATTATCCAAATCTTTCCTCCGGAAATTCATCGGAAGATGAACGGAGCGACCAGTTCTCCGATTCGGGAAATTCATCGGAAGACGACTCTGCCTCAGAGACCGTCCATTCGTCGATCTCCGACTCCGAAAGCGTCGCCGATTCGGGTTCTTCGAGTTCCGGTAATCAATCCCGGCCGTTCGGTGATGGGTTGGTCCCGCTTTTCGACGGGGACAGAGTTCACGACCTCATCAAGCAGCGGTTCCTTTCGAGTTTGGGATTGCTTGGGGCGCAAGCAAGTGTGGTGGCGGTTCACAGGAACTCCTACTCGAGCCTTGTTGGCCAAGCAAGGCTGCGTTCCTTTCAAATTTACTTGAAAGCCGTGGAGGAGAAGTGCGGGGGAAATCCCAACGTGAAGTACGCTTGGTTCGCGCCTTCTTCGAAAGACGAGATTTCGAAGATCATCTGCCATGGTTTCGGCAACTTTGAGCAGCCTCAGACAAATGGGGCGTTCGGAAACGGTGTTTATCTTGCTCCTGATGATTCTCCTCTGCAACTCGTCGAAAGCTTGAACGTTGACGAAGACGGTCTACGACATCTCCTGCTCTGCCGTGTGATACTGGGGAGGCCGGAGGTTGTGTGTCCCGGTTCTAAACAGCATCATCCGAGCTCCGAACAGTTTGATTCTGGCATCGACAGCATCTTTGCTCCCAAGAAGTACATAGTTTGGAGTACGCACATGAACACTCACATCCTGCCGGAGTATGTCATCAGCTTCAGAGCTCCTAATTGCTTGAAAGAACTTTTGAAGGTTCAAGAACCAGTTAAGAAACCGACTTCGCCGTGGATGCCGTTTCCGGCGCTAATCGGCGTGCTTTCGAAGTTCTTGCCTCCACCTTCCATTGCCTTGATCAGCAAGCATTACAAAGATCACAGAGAGAATGTGATTACAAGGCAGCAACTAATACAAAAAGTGAGACAAATTGCAGGGGACAAGTTACTGGCTTCAATTATCAAATCTTTCAGAGCCAAGCAAATCAAAACAATGCAAACTAAAGCGATGCCCCCAGCGGAGGCAGGAGTAAGATGGACGGTGTAG

Protein Analysis

364

Amino Acids

40.04

Weight (kDa)

6.83

Isoelectric Point (pI)

55.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 275 - 340 4.4e-27 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 583
AccII CGCG 1 cut(s) 425
AccIII TCCGGA 1 cut(s) 35
AciI CCGC 5 cut(s) 212, 253, 302, 390, 1064
AclI AACGTT 1 cut(s) 567
AcoI YGGCCR 2 cut(s) 188, 334
AcsI RAATTY 3 cut(s) 40, 85, 360
AcuI CTGAAG 1 cut(s) 754
AfaI GTAC 4 cut(s) 8, 413, 713, 727
AfiI CCNNNNNNNGG 1 cut(s) 1066
AgsI TTSAA 7 cut(s) 359, 370, 565, 790, 802, 809, 1002
AluBI AGCT 4 cut(s) 561, 663, 768, 776
AluI AGCT 4 cut(s) 561, 663, 768, 776
Alw21I GWGCWC 2 cut(s) 665, 778
Alw26I GTCTC 2 cut(s) 107, 964
Ama87I CYCGRG 1 cut(s) 322
Aor13HI TCCGGA 1 cut(s) 35
AoxI GGCC 3 cut(s) 188, 334, 620
ApeKI GCWGC 4 cut(s) 250, 346, 484, 949
ApoI RAATTY 3 cut(s) 40, 85, 360
ArsI GACNNNNNNTTYG 4 cut(s) 106, 138, 680, 712
AspLEI GCGC 3 cut(s) 286, 427, 856
AspS9I GGNCC 1 cut(s) 208
AsuC2I CCSGG 2 cut(s) 187, 639
AsuHPI GGTGA 1 cut(s) 209
AsuII TTCGAA 3 cut(s) 434, 449, 872
AvaI CYCGRG 1 cut(s) 322
AvaII GGWCC 1 cut(s) 208
BalI TGGCCA 1 cut(s) 336
BanII GRGCYC 2 cut(s) 665, 778
BarI GAAGNNNNNNTAC 2 cut(s) 567, 599
BbsI GAAGAC 2 cut(s) 102, 582
Bbv12I GWGCWC 2 cut(s) 665, 778
BbvI GCAGC 4 cut(s) 262, 333, 496, 961
BccI CCATC 2 cut(s) 194, 1074
BceAI ACGGC 5 cut(s) 175, 359, 588, 820, 829
BcgI CGANNNNNNTGC 2 cut(s) 862, 896
BclI TGATCA 1 cut(s) 903
BcnI CCSGG 2 cut(s) 187, 639
BcoDI GTCTC 2 cut(s) 107, 964
BfoI RGCGCY 1 cut(s) 857
BglI GCCNNNNNGGC 1 cut(s) 333
BisI GCNGC 4 cut(s) 251, 347, 485, 950
BlsI GCNGC 4 cut(s) 252, 348, 486, 951
Bme1390I CCNGG 2 cut(s) 187, 639
Bme18I GGWCC 1 cut(s) 208
BmeT110I CYCGRG 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 2 cut(s) 210, 257
BmrFI CCNGG 2 cut(s) 187, 639
BmrI ACTGGG 1 cut(s) 623
BmsI GCATC 5 cut(s) 661, 693, 702, 831, 1044
BmuI ACTGGG 1 cut(s) 623
BpiI GAAGAC 2 cut(s) 102, 582
Bpu14I TTCGAA 3 cut(s) 434, 449, 872
BpuMI CCSGG 2 cut(s) 187, 639
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 3 cut(s) 375, 463, 836
BsaWI WCCGGW 2 cut(s) 35, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 1066
Bse1I ACTGG 4 cut(s) 67, 618, 815, 999
Bse3DI GCAATG 1 cut(s) 894
BseAI TCCGGA 1 cut(s) 35
BseDI CCNNGG 3 cut(s) 375, 463, 836
BseGI GGATG 3 cut(s) 655, 744, 846
BseLI CCNNNNNNNGG 1 cut(s) 1066
BseMI GCAATG 1 cut(s) 894
BseMII CTCAG 2 cut(s) 123, 503
BseNI ACTGG 4 cut(s) 67, 618, 815, 999
BseRI GAGGAG 2 cut(s) 395, 531
BseX3I CGGCCG 1 cut(s) 188
BseXI GCAGC 4 cut(s) 262, 333, 496, 961
BseYI CCCAGC 1 cut(s) 1060
Bsh1236I CGCG 1 cut(s) 425
Bsh1285I CGRYCG 1 cut(s) 191
BshFI GGCC 3 cut(s) 190, 336, 622
BsiEI CGRYCG 1 cut(s) 191
BsiHKAI GWGCWC 2 cut(s) 665, 778
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 7 cut(s) 36, 174, 187, 623, 639, 752, 851
BslFI GGGAC 4 cut(s) 194, 239, 621, 998
BslI CCNNNNNNNGG 1 cut(s) 1066
BsmAI GTCTC 2 cut(s) 107, 964
BsmFI GGGAC 4 cut(s) 194, 239, 621, 998
BsnI GGCC 3 cut(s) 190, 336, 622
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 322
Bsp119I TTCGAA 3 cut(s) 434, 449, 872
Bsp1286I GDGCHC 2 cut(s) 665, 778
Bsp13I TCCGGA 1 cut(s) 35
Bsp143I GATC 4 cut(s) 129, 453, 903, 922
Bsp19I CCATGG 1 cut(s) 463
BspACI CCGC 5 cut(s) 212, 253, 302, 390, 1064
BspANI GGCC 3 cut(s) 190, 336, 622
BspCNI CTCAG 2 cut(s) 122, 502
BspEI TCCGGA 1 cut(s) 35
BspFNI CGCG 1 cut(s) 425
BspLI GGNNCC 2 cut(s) 210, 257
BspT104I TTCGAA 3 cut(s) 434, 449, 872
BspTNI GGTCTC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 894
BsrI ACTGG 4 cut(s) 67, 618, 815, 999
BssECI CCNNGG 3 cut(s) 375, 463, 836
BssMI GATC 4 cut(s) 129, 453, 903, 922
BssT1I CCWWGG 1 cut(s) 463
Bst4CI ACNGT 5 cut(s) 118, 515, 581, 672, 1087
BstBI TTCGAA 3 cut(s) 434, 449, 872
BstC8I GCNNGC 3 cut(s) 288, 866, 911
BstDEI CTNAG 2 cut(s) 109, 489
BstDSI CCRYGG 3 cut(s) 375, 463, 836
BstF5I GGATG 3 cut(s) 655, 744, 846
BstFNI CGCG 1 cut(s) 425
BstH2I RGCGCY 1 cut(s) 857
BstHHI GCGC 3 cut(s) 286, 427, 856
BstKTI GATC 4 cut(s) 132, 456, 906, 925
BstMAI GTCTC 2 cut(s) 107, 964
BstMBI GATC 4 cut(s) 129, 453, 903, 922
BstMCI CGRYCG 1 cut(s) 191
BstMWI GCNNNNNNNGC 5 cut(s) 283, 333, 422, 690, 841
BstSCI CCNGG 2 cut(s) 185, 637
BstUI CGCG 1 cut(s) 425
BstV1I GCAGC 4 cut(s) 262, 333, 496, 961
BstV2I GAAGAC 2 cut(s) 102, 582
BstZI CGGCCG 1 cut(s) 188
BsuRI GGCC 3 cut(s) 190, 336, 622
BtgI CCRYGG 3 cut(s) 375, 463, 836
BtgZI GCGATG 1 cut(s) 1067
BtsCI GGATG 3 cut(s) 655, 744, 846
Cac8I GCNNGC 3 cut(s) 288, 866, 911
CfoI GCGC 3 cut(s) 286, 427, 856
Cfr13I GGNCC 1 cut(s) 208
CseI GACGC 1 cut(s) 136
Csp6I GTAC 4 cut(s) 7, 412, 712, 726
CspCI CAANNNNNGTGG 2 cut(s) 279, 314
CviAII CATG 2 cut(s) 464, 733
CviQI GTAC 4 cut(s) 7, 412, 712, 726
DdeI CTNAG 2 cut(s) 109, 489
DpnI GATC 4 cut(s) 131, 455, 905, 924
DpnII GATC 4 cut(s) 129, 453, 903, 922
EaeI YGGCCR 2 cut(s) 188, 334
EagI CGGCCG 1 cut(s) 188
Ecl136II GAGCTC 2 cut(s) 663, 776
EclXI CGGCCG 1 cut(s) 188
Eco130I CCWWGG 1 cut(s) 463
Eco24I GRGCYC 2 cut(s) 665, 778
Eco31I GGTCTC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 208
Eco52I CGGCCG 1 cut(s) 188
Eco53kI GAGCTC 2 cut(s) 663, 776
Eco57I CTGAAG 1 cut(s) 754
Eco88I CYCGRG 1 cut(s) 322
EcoICRI GAGCTC 2 cut(s) 663, 776
EcoT14I CCWWGG 1 cut(s) 463
EcoT38I GRGCYC 2 cut(s) 665, 778
ErhI CCWWGG 1 cut(s) 463
FaeI CATG 2 cut(s) 467, 736
FaiI YATR 4 cut(s) 465, 716, 734, 759
FaqI GGGAC 4 cut(s) 194, 239, 621, 998
FatI CATG 2 cut(s) 463, 732
FauI CCCGC 2 cut(s) 219, 383
FbaI TGATCA 1 cut(s) 903
FblI GTMKAC 1 cut(s) 583
Fnu4HI GCNGC 4 cut(s) 251, 347, 485, 950
FokI GGATG 3 cut(s) 642, 731, 853
FriOI GRGCYC 2 cut(s) 665, 778
Fsp4HI GCNGC 4 cut(s) 251, 347, 485, 950
GlaI GCGC 3 cut(s) 285, 426, 855
GluI GCNGC 4 cut(s) 251, 347, 485, 950
GsaI CCCAGC 1 cut(s) 1064
HaeII RGCGCY 1 cut(s) 857
HaeIII GGCC 3 cut(s) 190, 336, 622
HapII CCGG 7 cut(s) 36, 174, 187, 623, 639, 752, 851
HgaI GACGC 1 cut(s) 136
HhaI GCGC 3 cut(s) 286, 427, 856
Hin1II CATG 2 cut(s) 467, 736
Hin6I GCGC 3 cut(s) 284, 425, 854
HinP1I GCGC 3 cut(s) 284, 425, 854
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HindIII AAGCTT 1 cut(s) 559
HinfI GANTC 6 cut(s) 77, 101, 137, 155, 536, 677
HpaII CCGG 7 cut(s) 36, 174, 187, 623, 639, 752, 851
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 4 cut(s) 235, 307, 571, 584
Hpy188III TCNNGA 5 cut(s) 36, 81, 236, 530, 809
Hpy8I GTNNAC 4 cut(s) 235, 307, 571, 584
Hpy99I CGWCG 4 cut(s) 130, 152, 224, 557
HpyAV CCTTC 3 cut(s) 438, 796, 900
HpyCH4III ACNGT 5 cut(s) 118, 515, 581, 672, 1087
HpyCH4IV ACGT 2 cut(s) 405, 567
HpyCH4V TGCA 3 cut(s) 547, 980, 1042
HpyF10VI GCNNNNNNNGC 5 cut(s) 283, 333, 422, 690, 841
HpyF3I CTNAG 2 cut(s) 109, 489
HpySE526I ACGT 2 cut(s) 405, 567
Hsp92II CATG 2 cut(s) 467, 736
HspAI GCGC 3 cut(s) 284, 425, 854
Kpn2I TCCGGA 1 cut(s) 35
Ksp22I TGATCA 1 cut(s) 903
Kzo9I GATC 4 cut(s) 129, 453, 903, 922
LmnI GCTCC 5 cut(s) 60, 532, 668, 706, 781
Lsp1109I GCAGC 4 cut(s) 262, 333, 496, 961
LweI GCATC 5 cut(s) 661, 693, 702, 831, 1044
MaeII ACGT 2 cut(s) 405, 567
MaeIII GTNAC 1 cut(s) 990
MalI GATC 4 cut(s) 131, 455, 905, 924
MboI GATC 4 cut(s) 129, 453, 903, 922
MboII GAAGA 6 cut(s) 62, 107, 156, 423, 463, 587
MhlI GDGCHC 2 cut(s) 665, 778
MlsI TGGCCA 1 cut(s) 336
MluCI AATT 6 cut(s) 40, 85, 360, 781, 975, 1002
MluNI TGGCCA 1 cut(s) 336
MlyI GAGTC 2 cut(s) 95, 131
MmeI TCCRAC 1 cut(s) 159
Mox20I TGGCCA 1 cut(s) 336
MroI TCCGGA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 336
MseI TTAA 1 cut(s) 819
Msp20I TGGCCA 1 cut(s) 336
MspA1I CMGCKG 2 cut(s) 253, 1064
MspI CCGG 7 cut(s) 36, 174, 187, 623, 639, 752, 851
MspR9I CCNGG 2 cut(s) 187, 639
MvnI CGCG 1 cut(s) 425
MwoI GCNNNNNNNGC 5 cut(s) 283, 333, 422, 690, 841
NciI CCSGG 2 cut(s) 187, 639
NcoI CCATGG 1 cut(s) 463
NdeII GATC 4 cut(s) 129, 453, 903, 922
NlaIII CATG 2 cut(s) 467, 736
NlaIV GGNNCC 2 cut(s) 210, 257
NspV TTCGAA 3 cut(s) 434, 449, 872
PaeR7I CTCGAG 1 cut(s) 322
PcsI WCGNNNNNNNCGW 1 cut(s) 164
PfeI GAWTC 4 cut(s) 77, 155, 536, 677
PkrI GCNGC 4 cut(s) 252, 348, 486, 951
PleI GAGTC 2 cut(s) 95, 131
PpsI GAGTC 2 cut(s) 95, 131
Psp124BI GAGCTC 2 cut(s) 665, 778
Psp1406I AACGTT 1 cut(s) 567
PspFI CCCAGC 1 cut(s) 1060
PspN4I GGNNCC 2 cut(s) 210, 257
PspPI GGNCC 1 cut(s) 208
PspXI VCTCGAGB 1 cut(s) 322
PsrI GAACNNNNNNTAC 2 cut(s) 404, 436
RsaI GTAC 4 cut(s) 8, 413, 713, 727
RsaNI GTAC 4 cut(s) 7, 412, 712, 726
SacI GAGCTC 2 cut(s) 665, 778
SaqAI TTAA 1 cut(s) 819
SatI GCNGC 4 cut(s) 251, 347, 485, 950
Sau3AI GATC 4 cut(s) 129, 453, 903, 922
Sau96I GGNCC 1 cut(s) 208
SchI GAGTC 2 cut(s) 95, 131
ScrFI CCNGG 2 cut(s) 187, 639
SduI GDGCHC 2 cut(s) 665, 778
SfaNI GCATC 5 cut(s) 661, 693, 702, 831, 1044
Sfr274I CTCGAG 1 cut(s) 322
SfuI TTCGAA 3 cut(s) 434, 449, 872
SinI GGWCC 1 cut(s) 208
SlaI CTCGAG 1 cut(s) 322
SmlI CTYRAG 1 cut(s) 322
SmoI CTYRAG 1 cut(s) 322
Sse9I AATT 6 cut(s) 40, 85, 360, 781, 975, 1002
SsiI CCGC 5 cut(s) 212, 253, 302, 390, 1064
SstI GAGCTC 2 cut(s) 665, 778
StyD4I CCNGG 2 cut(s) 185, 637
StyI CCWWGG 1 cut(s) 463
TaaI ACNGT 5 cut(s) 118, 515, 581, 672, 1087
TaiI ACGT 2 cut(s) 408, 570
TasI AATT 6 cut(s) 40, 85, 360, 781, 975, 1002
TatI WGTACW 2 cut(s) 6, 711
TfiI GAWTC 4 cut(s) 77, 155, 536, 677
Tru1I TTAA 1 cut(s) 819
Tru9I TTAA 1 cut(s) 819
TseI GCWGC 4 cut(s) 250, 346, 484, 949
TspDTI ATGAA 4 cut(s) 33, 69, 78, 749
TspGWI ACGGA 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 208
XapI RAATTY 3 cut(s) 40, 85, 360
XhoI CTCGAG 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.