FvH4_5g05860

Galactinol--sucrose galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005392
Physical Location & Seq
Forward (+)
3454389 .. 3458461
4073 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05860.t1

Sequence Viewer

Length: 2328 bp
ATGGCTCCAAGCCTGAGCAAGAACAATGCCCTAGACATATCGACCCTTGTAGACAGCAACTCTCCGCTCTCAATAACCCTACAAGGCACAACTTTCCTCGCCAACGGCCACCCTATCCTCACTGAAGTCCCGCTAAACATCACCGCCACCCCATCACCATACAAAACCACCGTAGGCTGCTTCGTTGGCTTCGACTCCGACGAGCCCAAAAGCCGCCATGTGGCGTCACTCGGCAAGCTCAGTGGCATCAAGTTCATGAGCATTTTCCGGTTCAAAGTCTGGTGGACCACCCACTGGGTCGGCACCACCGGAAACGACGTCCAGCACGAGACTCAGTTCATGATCCTCGACAAAAACGACACCACCAACCGCCCCTACGTCCTCCTCCTCCCTCTTCTCGAAGGTCCCTTCAGAGCCTCCCTCCAACCCGGCACCACCGACTGCCACGTCGATATGTGCGTCGAGAGCGGCTCGACGCGTGTCAGTGGCTCCACCTTCCGGAGCGTTCTCTACATGCACATCGGAAACGACCCGTACAGTCTTGTCAAAGAGGCCATGGAGGTGGTGAGAGTCCATTTGGGGAGCTTCAAGCTTCTGGAGGAGAAGACGCCTCCGGCCATCGTAGACAAGTTTGGTTGGTGCACTTGGGATGCGTTTTATCTCATGGTGCACCCCAAGGGGGTGTGGGAAGGGGTTAAAACCCTAGTGGAGGGTGGGTGTCCTCCGGGAATGGTACTCATCGACGACGGCTGGCAGTCCATTTGTCACGACGACGACCCCTTGACCGACGAAGAAGCCATGCAAAGAACAGCCGCCGGTGAGCAAATGCCATGCAGGCTGACAAGTTTCGTAGAGAACTACAAGTTCAGAGACTATAAGAGCCCTAGAGGTAAGGGCCATGGCATGGGTGCCTTTGTTAAGGACTTGAAGGAGGAGTTTGGGAGTGTGGACTATGTGTATGTTTGGCATGCTCTTTGTGGGTATTGGGGTGGAGTTCGACCCGGGGTTAAGGGCGGGTCGGGTTTTCCGGAGAGCCGGGTGATTAGGCCGAAGCTGTCGGAGGGGTTGCAGAAGACGATGGAGGATTTGGCGGTGGATAAGATCTTGAGTAACGGAGTGGGGCTGGTGGAACCGGAGATGGCCCAGCAGTTGTATGAGGGGCTTCATCAGCATCTCCAATCGGTGGGGATCGACGGTGTCAAAGTCGATGTCATACACTTGCTTGAGATGTTATCAGAGGAGTTTGGTGGTCGGATTGAGCTGGCCAAAGCTTATTACAAGGCACTTACTGCTTCTGTGAGGAAACATTTCAAAGGCAATGGAGTGATTGCTAGCATGGAGCACTGCAATGACTTCATGTTCCTCGGCACAGAAGCCATTTCGCTTGGTCGTGTTGGAGACGATTTTTGGTGCTCTGACCCATCTGGAGATCCAAATGGGACATATTGGCTACAAGGGTGTCATATGGTGCATTGTGCCTACAATAGCTTGTGGATGGGGAACTTCATACATCCAGATTGGGACATGTTTCAGTCGACTCACCCGTGTGCTGCATTTCATGCTGCATCAAGAGCCATTTCAGGAGGTCCGATATATATCAGTGACTCTGTGGGACAACACGACTTCAAGCTTCTTAAAAGCTTGGTGTTGCCTGATGGATCCATATTAAGATGCCAAGACTATGCACTTCCTTCAAGGGATTGCCTATTTCAGGACCCTGTGCATGATGGGAAAACAATGCTCAAGATTTGGAACCTCAACAAGTACACTGGCGTTCTAGGACTTTTTAACTGCCAAGGAGGAGGGTGGTGCCCCAAGTCAAGGCGCAACAAAAGCTTCCCCGAGTTCTCAAAGTTGGTGACCTGTTTAGCAACCCCAAAAGATGTGGAATGGAGTCATGGGAAGAACCCCATTCCAATAAAAGCAATCGAAGTATTTGCTGTGTACATGTTCCAGGAAAAGAAGCTGAAGTTTTTGAAGCCAGATGAGACGGTAGAGATTTCGCTTCAGCCTTTCAGTTATGAGCTCCTCACTATTGCTCCAGTGAGAGTGCTACCGAAGAAGTTGATCCAATTTGCTCCAATTGGATTGGTGAACATGCTCAACACTGGGGGTGCAATTCAGTCCCTGGAATTTGAGGATAGTGGAACTTTGGTGAAGATTGGAGTAAGGGGAAGTGGAGAGATGAGAGCATTTGCTTCAGAGAAACCAACTGCTTGTAAGATTGGTGGAGAGGAAGTCGAGTTTGATTATGTTGATAAGATGGTTATTATCCAAGTGCAATGGCCTAGCTCTTCAAGAACGACTGTAGTTGAGTACTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

776

Amino Acids

86.06

Weight (kDa)

5.96

Isoelectric Point (pI)

35.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 29 - 758 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 446
AatII GACGTC 1 cut(s) 321
AccB1I GGYRCC 4 cut(s) 302, 431, 908, 1809
AccB7I CCANNNNNTGG 3 cut(s) 294, 904, 1183
AccBSI CCGCTC 2 cut(s) 67, 468
AccI GTMKAC 3 cut(s) 51, 624, 1535
AccII CGCG 1 cut(s) 478
AccIII TCCGGA 2 cut(s) 498, 1027
AciI CCGC 9 cut(s) 65, 131, 144, 214, 370, 468, 813, 1014, 1091
AclWI GGATC 6 cut(s) 337, 1196, 1424, 1653, 1666, 2062
AcoI YGGCCR 3 cut(s) 106, 615, 1263
AcsI RAATTY 1 cut(s) 2132
AcuI CTGAAG 5 cut(s) 144, 394, 1988, 1991, 2184
AcyI GRCGYC 3 cut(s) 224, 318, 608
AfaI GTAC 5 cut(s) 536, 735, 1766, 1946, 2318
AfiI CCNNNNNNNGG 9 cut(s) 220, 294, 498, 679, 709, 904, 1183, 1711, 1821
AflIII ACRYGT 3 cut(s) 476, 1524, 1947
AgsI TTSAA 8 cut(s) 274, 589, 928, 1312, 1627, 1695, 1978, 2298
AjiI CACGTC 1 cut(s) 448
AjnI CCWGG 2 cut(s) 1953, 2127
AleI CACNNNNGTG 1 cut(s) 1545
Alw21I GWGCWC 5 cut(s) 644, 672, 1344, 1415, 2028
Alw26I GTCTC 4 cut(s) 323, 864, 1392, 1982
Alw44I GTGCAC 2 cut(s) 640, 668
AlwI GGATC 6 cut(s) 337, 1196, 1424, 1653, 1666, 2062
AlwNI CAGNNNCTG 1 cut(s) 2128
Ama87I CYCGRG 2 cut(s) 1001, 1841
Aor13HI TCCGGA 2 cut(s) 498, 1027
AoxI GGCC 8 cut(s) 106, 552, 615, 895, 1046, 1140, 1263, 2285
ApaLI GTGCAC 2 cut(s) 640, 668
ApeKI GCWGC 3 cut(s) 177, 1550, 1562
ApoI RAATTY 1 cut(s) 2132
ArsI GACNNNNNNTTYG 2 cut(s) 1194, 1226
Asp700I GAANNNNTTC 1 cut(s) 1307
AspLEI GCGC 1 cut(s) 1827
AspS9I GGNCC 6 cut(s) 285, 404, 895, 1141, 1586, 1714
AsuC2I CCSGG 5 cut(s) 429, 726, 1002, 1003, 1037
AsuHPI GGTGA 9 cut(s) 133, 147, 577, 830, 1051, 1532, 1870, 2104, 2167
AsuNHI GCTAGC 1 cut(s) 1331
AvaI CYCGRG 2 cut(s) 1001, 1841
AvaII GGWCC 4 cut(s) 285, 404, 1586, 1714
BaeGI GKGCMC 3 cut(s) 644, 672, 1814
BalI TGGCCA 1 cut(s) 1265
BamHI GGATCC 1 cut(s) 1658
BanI GGYRCC 4 cut(s) 302, 431, 908, 1809
BanII GRGCYC 3 cut(s) 207, 884, 2028
BauI CACGAG 1 cut(s) 326
BbsI GAAGAC 2 cut(s) 611, 1079
Bbv12I GWGCWC 5 cut(s) 644, 672, 1344, 1415, 2028
BbvI GCAGC 3 cut(s) 164, 1537, 1549
BccI CCATC 9 cut(s) 160, 626, 1072, 1132, 1429, 1489, 1649, 1721, 2257
BceAI ACGGC 2 cut(s) 121, 763
BcgI CGANNNNNNTGC 2 cut(s) 1919, 1953
BciT130I CCWGG 2 cut(s) 1955, 2129
BcnI CCSGG 5 cut(s) 429, 726, 1002, 1003, 1037
BcoDI GTCTC 4 cut(s) 323, 864, 1392, 1982
BfaI CTAG 6 cut(s) 32, 704, 885, 1332, 1778, 2289
BfmI CTRYAG 1 cut(s) 2307
BglI GCCNNNNNGGC 1 cut(s) 835
BglII AGATCT 1 cut(s) 1101
BisI GCNGC 6 cut(s) 178, 214, 469, 813, 1551, 1563
BlsI GCNGC 6 cut(s) 179, 215, 470, 814, 1552, 1564
BmcAI AGTACT 1 cut(s) 2318
Bme1390I CCNGG 7 cut(s) 429, 726, 1002, 1003, 1037, 1955, 2129
Bme18I GGWCC 4 cut(s) 285, 404, 1586, 1714
BmeT110I CYCGRG 2 cut(s) 1001, 1841
BmgBI CACGTC 1 cut(s) 448
BmgT120I GGNCC 6 cut(s) 285, 404, 895, 1141, 1586, 1714
BmrFI CCNGG 7 cut(s) 429, 726, 1002, 1003, 1037, 1955, 2129
BmrI ACTGGG 2 cut(s) 304, 2118
BmsI GCATC 5 cut(s) 255, 640, 1180, 1574, 1661
BmtI GCTAGC 1 cut(s) 1335
BmuI ACTGGG 2 cut(s) 304, 2118
BoxI GACNNNNGTC 1 cut(s) 479
BpiI GAAGAC 2 cut(s) 611, 1079
BplI GAGNNNNNCTC 4 cut(s) 405, 437, 455, 487
BpmI CTGGAG 3 cut(s) 617, 1446, 2025
Bpu10I CCTNAGC 1 cut(s) 14
BpuEI CTTGAG 3 cut(s) 1126, 1244, 1727
BpuMI CCSGG 5 cut(s) 429, 726, 1002, 1003, 1037
BsaBI GATNNNNATC 1 cut(s) 1595
BsaHI GRCGYC 3 cut(s) 224, 318, 608
BsaJI CCNNGG 8 cut(s) 555, 675, 898, 1001, 1002, 1363, 1795, 2127
BsaWI WCCGGW 5 cut(s) 267, 308, 498, 1027, 1132
BsaXI ACNNNNNCTCC 6 cut(s) 701, 731, 2023, 2053, 2223, 2253
Bsc4I CCNNNNNNNGG 9 cut(s) 220, 294, 498, 679, 709, 904, 1183, 1711, 1821
Bse118I RCCGGY 1 cut(s) 815
Bse1I ACTGG 4 cut(s) 299, 1774, 2042, 2113
Bse3DI GCAATG 3 cut(s) 1324, 1354, 2288
Bse8I GATNNNNATC 1 cut(s) 1595
BseAI TCCGGA 2 cut(s) 498, 1027
BseBI CCWGG 2 cut(s) 1955, 2129
BseDI CCNNGG 8 cut(s) 555, 675, 898, 1001, 1002, 1363, 1795, 2127
BseGI GGATG 3 cut(s) 655, 1500, 1510
BseJI GATNNNNATC 1 cut(s) 1595
BseLI CCNNNNNNNGG 9 cut(s) 220, 294, 498, 679, 709, 904, 1183, 1711, 1821
BseMI GCAATG 3 cut(s) 1324, 1354, 2288
BseMII CTCAG 3 cut(s) 5, 253, 347
BseNI ACTGG 4 cut(s) 299, 1774, 2042, 2113
BseRI GAGGAG 7 cut(s) 374, 377, 614, 947, 1253, 1815, 2018
BseSI GKGCMC 3 cut(s) 644, 672, 1814
BseXI GCAGC 3 cut(s) 164, 1537, 1549
BseYI CCCAGC 1 cut(s) 1143
Bsh1236I CGCG 1 cut(s) 478
BshFI GGCC 8 cut(s) 108, 554, 617, 897, 1048, 1142, 1265, 2287
BshNI GGYRCC 4 cut(s) 302, 431, 908, 1809
BsiHKAI GWGCWC 5 cut(s) 644, 672, 1344, 1415, 2028
BsiHKCI CYCGRG 2 cut(s) 1001, 1841
BslFI GGGAC 6 cut(s) 113, 390, 1453, 1535, 1626, 2110
BslI CCNNNNNNNGG 9 cut(s) 220, 294, 498, 679, 709, 904, 1183, 1711, 1821
BsmAI GTCTC 4 cut(s) 323, 864, 1392, 1982
BsmBI CGTCTC 2 cut(s) 1392, 1982
BsmFI GGGAC 6 cut(s) 113, 390, 1453, 1535, 1626, 2110
BsnI GGCC 8 cut(s) 108, 554, 617, 897, 1048, 1142, 1265, 2287
BsoBI CYCGRG 2 cut(s) 1001, 1841
Bsp1286I GDGCHC 8 cut(s) 207, 644, 672, 884, 1344, 1415, 1814, 2028
Bsp13I TCCGGA 2 cut(s) 498, 1027
Bsp1407I TGTACA 1 cut(s) 1944
Bsp143I GATC 6 cut(s) 342, 1101, 1188, 1429, 1658, 2067
Bsp19I CCATGG 2 cut(s) 555, 898
BspACI CCGC 9 cut(s) 65, 131, 144, 214, 370, 468, 813, 1014, 1091
BspANI GGCC 8 cut(s) 108, 554, 617, 897, 1048, 1142, 1265, 2287
BspCNI CTCAG 3 cut(s) 6, 252, 346
BspEI TCCGGA 2 cut(s) 498, 1027
BspFNI CGCG 1 cut(s) 478
BspHI TCATGA 2 cut(s) 255, 339
BspOI GCTAGC 1 cut(s) 1335
BspPI GGATC 6 cut(s) 337, 1196, 1424, 1653, 1666, 2062
BspQI GCTCTTC 1 cut(s) 2299
BspT107I GGYRCC 4 cut(s) 302, 431, 908, 1809
BsrBI CCGCTC 2 cut(s) 67, 468
BsrDI GCAATG 3 cut(s) 1324, 1354, 2288
BsrFI RCCGGY 1 cut(s) 815
BsrGI TGTACA 1 cut(s) 1944
BsrI ACTGG 4 cut(s) 299, 1774, 2042, 2113
BssAI RCCGGY 1 cut(s) 815
BssECI CCNNGG 8 cut(s) 555, 675, 898, 1001, 1002, 1363, 1795, 2127
BssMI GATC 6 cut(s) 342, 1101, 1188, 1429, 1658, 2067
BssNI GRCGYC 3 cut(s) 224, 318, 608
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 4 cut(s) 555, 675, 898, 1795
Bst2BI CACGAG 1 cut(s) 326
Bst2UI CCWGG 2 cut(s) 1955, 2129
Bst4CI ACNGT 5 cut(s) 172, 539, 1196, 1993, 2308
Bst6I CTCTTC 2 cut(s) 399, 2299
BstACI GRCGYC 3 cut(s) 224, 318, 608
BstAPI GCANNNNNTGC 2 cut(s) 1289, 1559
BstAUI TGTACA 1 cut(s) 1944
BstC8I GCNNGC 6 cut(s) 236, 752, 836, 969, 1263, 1333
BstDEI CTNAG 3 cut(s) 14, 239, 333
BstDSI CCRYGG 2 cut(s) 555, 898
BstEII GGTNACC 1 cut(s) 1858
BstENI CCTNNNNNAGG 2 cut(s) 707, 1709
BstF5I GGATG 3 cut(s) 655, 1500, 1510
BstFNI CGCG 1 cut(s) 478
BstHHI GCGC 1 cut(s) 1827
BstKTI GATC 6 cut(s) 345, 1104, 1191, 1432, 1661, 2070
BstMAI GTCTC 4 cut(s) 323, 864, 1392, 1982
BstMBI GATC 6 cut(s) 342, 1101, 1188, 1429, 1658, 2067
BstMWI GCNNNNNNNGC 8 cut(s) 186, 465, 835, 1168, 1289, 1559, 1571, 1833
BstNI CCWGG 2 cut(s) 1955, 2129
BstNSI RCATGY 5 cut(s) 517, 971, 1528, 1951, 2101
BstPAI GACNNNNGTC 1 cut(s) 479
BstPI GGTNACC 1 cut(s) 1858
BstSCI CCNGG 7 cut(s) 427, 724, 1000, 1001, 1035, 1953, 2127
BstSFI CTRYAG 1 cut(s) 2307
BstSLI GKGCMC 3 cut(s) 644, 672, 1814
BstUI CGCG 1 cut(s) 478
BstV1I GCAGC 3 cut(s) 164, 1537, 1549
BstV2I GAAGAC 2 cut(s) 611, 1079
BstX2I RGATCY 3 cut(s) 1101, 1429, 1658
BstXI CCANNNNNNTGG 1 cut(s) 562
BstYI RGATCY 3 cut(s) 1101, 1429, 1658
BsuRI GGCC 8 cut(s) 108, 554, 617, 897, 1048, 1142, 1265, 2287
BtgI CCRYGG 2 cut(s) 555, 898
BtrI CACGTC 1 cut(s) 448
BtsCI GGATG 3 cut(s) 655, 1500, 1510
BtsI GCAGTG 1 cut(s) 1342
BtsIMutI CAGTG 9 cut(s) 120, 247, 292, 490, 1342, 1606, 1767, 2049, 2106
Cac8I GCNNGC 6 cut(s) 236, 752, 836, 969, 1263, 1333
CaiI CAGNNNCTG 1 cut(s) 2128
CciI TCATGA 2 cut(s) 255, 339
CfoI GCGC 1 cut(s) 1827
Cfr10I RCCGGY 1 cut(s) 815
Cfr13I GGNCC 6 cut(s) 285, 404, 895, 1141, 1586, 1714
Cfr9I CCCGGG 1 cut(s) 1001
CseI GACGC 4 cut(s) 213, 448, 484, 616
Csp6I GTAC 5 cut(s) 535, 734, 1765, 1945, 2317
CspCI CAANNNNNGTGG 4 cut(s) 223, 258, 1866, 1901
CviQI GTAC 5 cut(s) 535, 734, 1765, 1945, 2317
DdeI CTNAG 3 cut(s) 14, 239, 333
DpnI GATC 6 cut(s) 344, 1103, 1190, 1431, 1660, 2069
DpnII GATC 6 cut(s) 342, 1101, 1188, 1429, 1658, 2067
DrdI GACNNNNNNGTC 1 cut(s) 446
DseDI GACNNNNNNGTC 1 cut(s) 446
EaeI YGGCCR 3 cut(s) 106, 615, 1263
Eam1104I CTCTTC 2 cut(s) 399, 2299
EarI CTCTTC 2 cut(s) 399, 2299
Ecl136II GAGCTC 1 cut(s) 2026
Eco130I CCWWGG 4 cut(s) 555, 675, 898, 1795
Eco24I GRGCYC 3 cut(s) 207, 884, 2028
Eco47I GGWCC 4 cut(s) 285, 404, 1586, 1714
Eco53kI GAGCTC 1 cut(s) 2026
Eco57I CTGAAG 5 cut(s) 144, 394, 1988, 1991, 2184
Eco88I CYCGRG 2 cut(s) 1001, 1841
Eco91I GGTNACC 1 cut(s) 1858
EcoICRI GAGCTC 1 cut(s) 2026
EcoNI CCTNNNNNAGG 2 cut(s) 707, 1709
EcoO109I RGGNCCY 2 cut(s) 404, 1714
EcoO65I GGTNACC 1 cut(s) 1858
EcoRII CCWGG 2 cut(s) 1953, 2127
EcoT14I CCWWGG 4 cut(s) 555, 675, 898, 1795
EcoT38I GRGCYC 3 cut(s) 207, 884, 2028
ErhI CCWWGG 4 cut(s) 555, 675, 898, 1795
Esp3I CGTCTC 2 cut(s) 1392, 1982
FaqI GGGAC 6 cut(s) 113, 390, 1453, 1535, 1626, 2110
FauI CCCGC 2 cut(s) 138, 1007
FauNDI CATATG 1 cut(s) 1464
FblI GTMKAC 3 cut(s) 51, 624, 1535
Fnu4HI GCNGC 6 cut(s) 178, 214, 469, 813, 1551, 1563
FokI GGATG 3 cut(s) 662, 1497, 1507
FriOI GRGCYC 3 cut(s) 207, 884, 2028
Fsp4HI GCNGC 6 cut(s) 178, 214, 469, 813, 1551, 1563
FspBI CTAG 6 cut(s) 32, 704, 885, 1332, 1778, 2289
GlaI GCGC 1 cut(s) 1826
GluI GCNGC 6 cut(s) 178, 214, 469, 813, 1551, 1563
GsaI CCCAGC 1 cut(s) 1147
GsuI CTGGAG 3 cut(s) 617, 1446, 2025
HaeIII GGCC 8 cut(s) 108, 554, 617, 897, 1048, 1142, 1265, 2287
HgaI GACGC 4 cut(s) 213, 448, 484, 616
HhaI GCGC 1 cut(s) 1827
Hin1I GRCGYC 3 cut(s) 224, 318, 608
Hin6I GCGC 1 cut(s) 1825
HinP1I GCGC 1 cut(s) 1825
HincII GTYRAC 1 cut(s) 1536
HindII GTYRAC 1 cut(s) 1536
HindIII AAGCTT 5 cut(s) 590, 1269, 1628, 1639, 1834
HinfI GANTC 6 cut(s) 194, 331, 570, 1537, 1604, 1894
HphI GGTGA 9 cut(s) 133, 147, 577, 830, 1051, 1532, 1870, 2104, 2167
HpyAV CCTTC 6 cut(s) 395, 418, 505, 683, 922, 1701
HpyCH4III ACNGT 5 cut(s) 172, 539, 1196, 1993, 2308
HpyCH4IV ACGT 3 cut(s) 318, 378, 447
HpyF10VI GCNNNNNNNGC 8 cut(s) 186, 465, 835, 1168, 1289, 1559, 1571, 1833
HpyF3I CTNAG 3 cut(s) 14, 239, 333
HpySE526I ACGT 3 cut(s) 318, 378, 447
Hsp92I GRCGYC 3 cut(s) 224, 318, 608
HspAI GCGC 1 cut(s) 1825
Kpn2I TCCGGA 2 cut(s) 498, 1027
Kzo9I GATC 6 cut(s) 342, 1101, 1188, 1429, 1658, 2067
LguI GCTCTTC 1 cut(s) 2299
LmnI GCTCC 8 cut(s) 10, 494, 501, 582, 1339, 2031, 2044, 2083
Lsp1109I GCAGC 3 cut(s) 164, 1537, 1549
LweI GCATC 5 cut(s) 255, 640, 1180, 1574, 1661
MaeI CTAG 6 cut(s) 32, 704, 885, 1332, 1778, 2289
MaeII ACGT 3 cut(s) 318, 378, 447
MaeIII GTNAC 5 cut(s) 225, 764, 1109, 1601, 1858
MalI GATC 6 cut(s) 344, 1103, 1190, 1431, 1660, 2069
MbiI CCGCTC 2 cut(s) 67, 468
MboI GATC 6 cut(s) 342, 1101, 1188, 1429, 1658, 2067
MboII GAAGA 8 cut(s) 386, 616, 803, 1084, 1915, 2071, 2170, 2286
MfeI CAATTG 1 cut(s) 2082
MflI RGATCY 3 cut(s) 1101, 1429, 1658
MhlI GDGCHC 8 cut(s) 207, 644, 672, 884, 1344, 1415, 1814, 2028
MlsI TGGCCA 1 cut(s) 1265
MluCI AATT 4 cut(s) 2072, 2082, 2118, 2132
MluI ACGCGT 1 cut(s) 476
MluNI TGGCCA 1 cut(s) 1265
MlyI GAGTC 6 cut(s) 188, 325, 579, 1531, 1598, 1903
MmeI TCCRAC 5 cut(s) 222, 448, 1038, 1232, 1375
Mox20I TGGCCA 1 cut(s) 1265
MroI TCCGGA 2 cut(s) 498, 1027
MroXI GAANNNNTTC 1 cut(s) 1307
MscI TGGCCA 1 cut(s) 1265
MseI TTAA 6 cut(s) 696, 918, 1008, 1635, 1667, 1788
MslI CAYNNNNRTG 3 cut(s) 560, 1347, 1545
Msp20I TGGCCA 1 cut(s) 1265
MspR9I CCNGG 7 cut(s) 429, 726, 1002, 1003, 1037, 1955, 2129
MunI CAATTG 1 cut(s) 2082
MvaI CCWGG 2 cut(s) 1955, 2129
MvnI CGCG 1 cut(s) 478
MwoI GCNNNNNNNGC 8 cut(s) 186, 465, 835, 1168, 1289, 1559, 1571, 1833
NciI CCSGG 5 cut(s) 429, 726, 1002, 1003, 1037
NcoI CCATGG 2 cut(s) 555, 898
NdeI CATATG 1 cut(s) 1464
NdeII GATC 6 cut(s) 342, 1101, 1188, 1429, 1658, 2067
NheI GCTAGC 1 cut(s) 1331
NmeAIII GCCGAG 2 cut(s) 210, 1344
NmuCI GTSAC 4 cut(s) 225, 764, 1601, 1858
NspI RCATGY 5 cut(s) 517, 971, 1528, 1951, 2101
OliI CACNNNNGTG 1 cut(s) 1545
PaeI GCATGC 1 cut(s) 971
PagI TCATGA 2 cut(s) 255, 339
PciI ACATGT 2 cut(s) 1524, 1947
PciSI GCTCTTC 1 cut(s) 2299
PcsI WCGNNNNNNNCGW 6 cut(s) 189, 198, 324, 354, 456, 1541
PdmI GAANNNNTTC 1 cut(s) 1307
PflFI GACNNNGTC 1 cut(s) 1196
PflMI CCANNNNNTGG 3 cut(s) 294, 904, 1183
PfoI TCCNGGA 2 cut(s) 724, 1953
PkrI GCNGC 6 cut(s) 179, 215, 470, 814, 1552, 1564
PleI GAGTC 6 cut(s) 188, 325, 578, 1531, 1598, 1902
PpsI GAGTC 6 cut(s) 188, 325, 578, 1531, 1598, 1902
PpuMI RGGWCCY 2 cut(s) 404, 1714
PscI ACATGT 2 cut(s) 1524, 1947
PshAI GACNNNNGTC 1 cut(s) 479
Psp124BI GAGCTC 1 cut(s) 2028
Psp5II RGGWCCY 2 cut(s) 404, 1714
Psp6I CCWGG 2 cut(s) 1953, 2127
PspEI GGTNACC 1 cut(s) 1858
PspFI CCCAGC 1 cut(s) 1143
PspGI CCWGG 2 cut(s) 1953, 2127
PspPI GGNCC 6 cut(s) 285, 404, 895, 1141, 1586, 1714
PspPPI RGGWCCY 2 cut(s) 404, 1714
PstNI CAGNNNCTG 1 cut(s) 2128
PsuI RGATCY 3 cut(s) 1101, 1429, 1658
PsyI GACNNNGTC 1 cut(s) 1196
RsaI GTAC 5 cut(s) 536, 735, 1766, 1946, 2318
RsaNI GTAC 5 cut(s) 535, 734, 1765, 1945, 2317
RseI CAYNNNNRTG 3 cut(s) 560, 1347, 1545
SacI GAGCTC 1 cut(s) 2028
SalI GTCGAC 1 cut(s) 1534
SapI GCTCTTC 1 cut(s) 2299
SaqAI TTAA 6 cut(s) 696, 918, 1008, 1635, 1667, 1788
SatI GCNGC 6 cut(s) 178, 214, 469, 813, 1551, 1563
Sau3AI GATC 6 cut(s) 342, 1101, 1188, 1429, 1658, 2067
Sau96I GGNCC 6 cut(s) 285, 404, 895, 1141, 1586, 1714
ScaI AGTACT 1 cut(s) 2318
SchI GAGTC 6 cut(s) 188, 325, 579, 1531, 1598, 1903
ScrFI CCNGG 7 cut(s) 429, 726, 1002, 1003, 1037, 1955, 2129
SduI GDGCHC 8 cut(s) 207, 644, 672, 884, 1344, 1415, 1814, 2028
SfaNI GCATC 5 cut(s) 255, 640, 1180, 1574, 1661
SfcI CTRYAG 1 cut(s) 2307
SgrAI CRCCGGYG 1 cut(s) 815
SinI GGWCC 4 cut(s) 285, 404, 1586, 1714
SmaI CCCGGG 1 cut(s) 1003
SmiMI CAYNNNNRTG 3 cut(s) 560, 1347, 1545
SmlI CTYRAG 3 cut(s) 1105, 1223, 1742
SmoI CTYRAG 3 cut(s) 1105, 1223, 1742
SphI GCATGC 1 cut(s) 971
Sse9I AATT 4 cut(s) 2072, 2082, 2118, 2132
SsiI CCGC 9 cut(s) 65, 131, 144, 214, 370, 468, 813, 1014, 1091
SspMI CTAG 6 cut(s) 32, 704, 885, 1332, 1778, 2289
SstI GAGCTC 1 cut(s) 2028
StyD4I CCNGG 7 cut(s) 427, 724, 1000, 1001, 1035, 1953, 2127
StyI CCWWGG 4 cut(s) 555, 675, 898, 1795
TaaI ACNGT 5 cut(s) 172, 539, 1196, 1993, 2308
TaiI ACGT 3 cut(s) 321, 381, 450
TaqII GACCGA 1 cut(s) 800
TasI AATT 4 cut(s) 2072, 2082, 2118, 2132
TatI WGTACW 3 cut(s) 1764, 1944, 2316
TauI GCSGC 3 cut(s) 216, 471, 815
Tru1I TTAA 6 cut(s) 696, 918, 1008, 1635, 1667, 1788
Tru9I TTAA 6 cut(s) 696, 918, 1008, 1635, 1667, 1788
TscAI CASTG 9 cut(s) 127, 247, 299, 490, 1349, 1606, 1774, 2049, 2113
TseFI GTSAC 4 cut(s) 225, 764, 1601, 1858
TseI GCWGC 3 cut(s) 177, 1550, 1562
Tsp45I GTSAC 4 cut(s) 225, 764, 1601, 1858
TspDTI ATGAA 6 cut(s) 244, 328, 1154, 1345, 1495, 1547
TspGWI ACGGA 1 cut(s) 1128
TspMI CCCGGG 1 cut(s) 1001
TspRI CASTG 9 cut(s) 127, 247, 299, 490, 1349, 1606, 1774, 2049, 2113
Tth111I GACNNNGTC 1 cut(s) 1196
Van91I CCANNNNNTGG 3 cut(s) 294, 904, 1183
VneI GTGCAC 2 cut(s) 640, 668
VpaK11BI GGWCC 4 cut(s) 285, 404, 1586, 1714
XagI CCTNNNNNAGG 2 cut(s) 707, 1709
XapI RAATTY 1 cut(s) 2132
XceI RCATGY 5 cut(s) 517, 971, 1528, 1951, 2101
XmaI CCCGGG 1 cut(s) 1001
XmiI GTMKAC 3 cut(s) 51, 624, 1535
XmnI GAANNNNTTC 1 cut(s) 1307
XspI CTAG 6 cut(s) 32, 704, 885, 1332, 1778, 2289
ZraI GACGTC 1 cut(s) 319
ZrmI AGTACT 1 cut(s) 2318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.