MD14G1175300.v1.1

Galactinol--sucrose galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005392
Physical Location & Seq
Reverse (-)
26880353 .. 26885070
4718 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1175300.v1.1.491

Sequence Viewer

Length: 2322 bp
ATGGCTTCAAGCATAACTAAGATTAACGTAGCCCTTGTGAATGACACGTCACCACTCCCAATTACTCTCAAAGGATCCAACCTTCTCGCTAATGGCCACCCAATCCTCACTGAAGTCCCTCTCAACATCACAGCTACCCTGTCACCTTTTAACAAGACCCCCTCCGGCTGCTTCCTTGGCTTCGACGCCGACGAGCCCAGGAGCCGCCACGTGGTCCACATCGGAAAACTCACTGAGATCAAGTTCATGAGCATATTCCGGTTCAAGGTCTGGTGGGCCACCCGATGTACCGGAACCACCGGAAACGACTTGGAGCACGAGACCCAGATGATGCTCTTGGACAAAAACGACTCCGGGCTCCCTTACGTTGTGATTCTTCCACTTCTGGAAGGACCCTTCAGAGCCTCTCTCCAACCGAGTCATGCAAACGATGACTACGTGGACATTTGCATGGAAAGCGGCTCGACACGTGTCAGCGCATCGAGCTTCAGGAGCTGCTTGTACATGCAAGTTGGCGGTGATGACCCGTATAGCTTGGTCAAGGAGGCAATGAAGGTGGTGAGGGCCCACTTGGGGAAGTTCAAGCTCCTAGAGGAAAAAACTGTACCAAAAATAGTGGACAAGTTTGGGTGGTGTACGTGGGATGCATTCTATCTTATGGTGCACCCCAGAGGGGTGTGGGAAGGTGTGAAGGGCCTGGTGGAGGGTGGGTGCCCTCCAGGACTGGTCCTCATTGATGACGGGTGGCAATCAATCAGTCTTGACGAGGACTCAGTGGAGGGCCAAGAAGGCATGGCCAGGATATCAGCTGGGGAACAAATGCCATGCAGGCTGATTAGTTTTAAAGAGAATTATAAATTTAAGGACTATGAGGGGGCGTCTGGCAAGGGCTTGGGTGGCTTTGTAAAGGATTTGAAGGAGGAATTTGGGAGTGTGGAGCATGTGTATGTGTGGCATGCGCTTTGTGGGTATTGGGGTGGGATTAGGCCTGGGATTAATAGTCCAGGGATGCCCGAAGAGTGCAGGATGGTTAGTCCTAAGTTGTCTCCGGGATTGCGGAGGACAATGGAGGACCCGGCGGTGGAGAAGATTGTGAGGAATGGAGTTGGGTTGGTTCTGCCGGAGGTGGCCCACAAGTTATATGAGGGGATTCACTCTCATCTTCAGTCGGTGGGGATTGATGGTGTCAAGGTTGATGTCATAAACTTGCTTGAGATGTTATCCGAGGATTTTGGCGGTCAGGTTGAGCTGGCTAAAGCATATTACAAGGCACTGACTGCTTCTGTGAGGAAGCATTTCAATGGCAATGGAGTGATTGCTAGCATGCAGCACAGCAATGACTTTATGTACCTCGGAACGGAGGCCATAGCCCTTGGACTTGTTGGAGATGATTTCTGTTCTGAGACGATGGAGCAAGCAGGTGGGACATATTGGTTGCAAGGGTGTCACGTGGTGAACTGTGCCTACAATAGCCTTTGGATAGGCAACATCATACAGCCAGATTGGGACATGTTTCAATCGACACACCCGTGCGCTGAGTTCCATGCTGCATCAAGAGCCATATCTGGAGGACCAATCTACATTAGTGACTCTGTTGGAAAGCACAACTTCAAGCTTCTTAAGAACCTGGTGTTGCCAGATGGGTCCGTACTGCGATGCCAACACTATGCCCTTCCTTCTAGGGATTGCCTCTTTGAAGACCCTGTGCATGATGGGAAAACAATGCTCAAGATATGGAACCTCAACAAATACACTGGAGTTTTAGGTGCTTTTAATTGTCAAGGCGGAGGATGGTGCTCTAAATCCAGGCGTAACAAAATTGCCCCCGACTGCTCAAAGTCTGTGACCTGTTTGGCTAGCCCCAAAGATGTCGAATGGAATCAAGGGAAGAATCCAATTTCAATCAAAGGAGTGAACACATTTGCTGTGTACATGTTCCAGCAAAACAAGCTGAAGCTCTTGCAGCCATCAGAGAAGATAGAGATTTCACTCGACCCTTTTACTTTTGAGCTCCTCACCGTTTCGCCGGTGAGGGTCTTGCCGAAGAAACTGATCCAATTTTCTCCGATTGGATTGGTCAACATGCTTAACACTGGCGGTGCAATTGAGTCAGTAGAATTTGAGGAAGACGAAGATTCCCTTAGTTTGGTGAGAATTGGAGTGAAGGGTTGTGGAGAAATGAGAATGTTTGCTTCAGAAAAGCCAATTGCTTGTAAGATCGATGGGGAGGGAGTGAAGTTTCATTACGTTGATAAGATGGTCAAAGTCCAGGTGCCATGGCTTAGTTCTTCAAGGTCAACTTTAGTTGAGTACCTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

774

Amino Acids

85.38

Weight (kDa)

6.06

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 756 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
AarI CACCTGC 1 cut(s) 1409
Acc36I ACCTGC 1 cut(s) 1409
AccB1I GGYRCC 2 cut(s) 711, 2272
AciI CCGC 8 cut(s) 205, 459, 516, 1057, 1079, 1236, 1785, 2097
AclWI GGATC 3 cut(s) 69, 82, 2047
AcoI YGGCCR 2 cut(s) 94, 795
AcsI RAATTY 3 cut(s) 857, 923, 2117
AcuI CTGAAG 6 cut(s) 132, 382, 472, 1148, 1973, 2178
AcvI CACGTG 3 cut(s) 211, 470, 1450
AcyI GRCGYC 2 cut(s) 186, 878
AdeI CACNNNGTG 1 cut(s) 1453
AfaI GTAC 8 cut(s) 289, 503, 606, 637, 1349, 1650, 1931, 2312
AfiI CCNNNNNNNGG 8 cut(s) 211, 265, 573, 673, 703, 1081, 1357, 2146
AflII CTTAAG 1 cut(s) 1619
AflIII ACRYGT 5 cut(s) 45, 467, 469, 1509, 1932
AjiI CACGTC 1 cut(s) 48
AjnI CCWGG 9 cut(s) 197, 696, 718, 797, 988, 1003, 1626, 1805, 2268
AleI CACNNNNGTG 1 cut(s) 1528
Alw21I GWGCWC 4 cut(s) 318, 666, 1799, 2013
Alw26I GTCTC 3 cut(s) 314, 1050, 1397
Alw44I GTGCAC 1 cut(s) 662
AlwI GGATC 3 cut(s) 69, 82, 2047
AlwNI CAGNNNCTG 1 cut(s) 495
AoxI GGCC 9 cut(s) 94, 276, 564, 694, 781, 795, 986, 1128, 1362
ApaI GGGCCC 1 cut(s) 568
ApaLI GTGCAC 1 cut(s) 662
ApeKI GCWGC 5 cut(s) 168, 495, 1327, 1547, 1963
ApoI RAATTY 3 cut(s) 857, 923, 2117
AseI ATTAAT 1 cut(s) 996
Asp700I GAANNNNTTC 1 cut(s) 1295
AspLEI GCGC 3 cut(s) 479, 961, 1535
AsuC2I CCSGG 3 cut(s) 355, 1050, 1076
AsuHPI GGTGA 8 cut(s) 42, 135, 530, 571, 1465, 2008, 2041, 2161
AsuNHI GCTAGC 2 cut(s) 1319, 1856
AvaII GGWCC 6 cut(s) 214, 392, 727, 1072, 1571, 1644
BaeGI GKGCMC 3 cut(s) 568, 666, 716
BalI TGGCCA 2 cut(s) 96, 797
BamHI GGATCC 1 cut(s) 74
BanI GGYRCC 2 cut(s) 711, 2272
BanII GRGCYC 4 cut(s) 198, 360, 568, 2013
BarI GAAGNNNNNNTAC 2 cut(s) 2228, 2260
BauI CACGAG 1 cut(s) 317
BbrPI CACGTG 3 cut(s) 211, 470, 1450
BbsI GAAGAC 2 cut(s) 1704, 2133
Bbv12I GWGCWC 4 cut(s) 318, 666, 1799, 2013
BbvI GCAGC 5 cut(s) 155, 482, 1339, 1534, 1975
BccI CCATC 9 cut(s) 1021, 1175, 1402, 1634, 1706, 1785, 1975, 2216, 2251
BciT130I CCWGG 9 cut(s) 199, 698, 720, 799, 990, 1005, 1628, 1807, 2270
BcnI CCSGG 3 cut(s) 355, 1050, 1076
BcoDI GTCTC 3 cut(s) 314, 1050, 1397
BfaI CTAG 4 cut(s) 590, 1320, 1680, 1857
BfrI CTTAAG 1 cut(s) 1619
BfuAI ACCTGC 1 cut(s) 1409
BglI GCCNNNNNGGC 2 cut(s) 789, 829
BisI GCNGC 7 cut(s) 169, 205, 460, 496, 1328, 1548, 1964
BlsI GCNGC 7 cut(s) 170, 206, 461, 497, 1329, 1549, 1965
Bme18I GGWCC 6 cut(s) 214, 392, 727, 1072, 1571, 1644
BmgBI CACGTC 1 cut(s) 48
BmsI GCATC 6 cut(s) 321, 488, 634, 999, 1559, 1646
BmtI GCTAGC 2 cut(s) 1323, 1860
BoxI GACNNNNGTC 1 cut(s) 470
BpiI GAAGAC 2 cut(s) 1704, 2133
BplI GAGNNNNNCTC 2 cut(s) 393, 425
BpmI CTGGAG 3 cut(s) 702, 1587, 1776
BpuEI CTTGAG 2 cut(s) 1232, 1712
BpuMI CCSGG 3 cut(s) 355, 1050, 1076
Bsa29I ATCGAT 1 cut(s) 2220
BsaAI YACGTR 5 cut(s) 211, 439, 470, 639, 1450
BsaHI GRCGYC 2 cut(s) 186, 878
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 8 cut(s) 175, 197, 989, 1004, 1224, 1351, 1372, 2276
BsaWI WCCGGW 3 cut(s) 258, 290, 299
BsaXI ACNNNNNCTCC 4 cut(s) 695, 725, 929, 959
Bsc4I CCNNNNNNNGG 8 cut(s) 211, 265, 573, 673, 703, 1081, 1357, 2146
Bse118I RCCGGY 1 cut(s) 2026
Bse1I ACTGG 3 cut(s) 729, 1759, 2098
Bse3DI GCAATG 3 cut(s) 555, 1312, 1342
BseBI CCWGG 9 cut(s) 199, 698, 720, 799, 990, 1005, 1628, 1807, 2270
BseCI ATCGAT 1 cut(s) 2220
BseDI CCNNGG 8 cut(s) 175, 197, 989, 1004, 1224, 1351, 1372, 2276
BseGI GGATG 4 cut(s) 649, 1014, 1032, 1796
BseLI CCNNNNNNNGG 8 cut(s) 211, 265, 573, 673, 703, 1081, 1357, 2146
BseMI GCAATG 3 cut(s) 555, 1312, 1342
BseMII CTCAG 4 cut(s) 225, 786, 1392, 1527
BseNI ACTGG 3 cut(s) 729, 1759, 2098
BseRI GAGGAG 1 cut(s) 2003
BseSI GKGCMC 3 cut(s) 568, 666, 716
BseXI GCAGC 5 cut(s) 155, 482, 1339, 1534, 1975
BseYI CCCAGC 1 cut(s) 809
BsgI GTGCAG 1 cut(s) 1042
BshFI GGCC 9 cut(s) 96, 278, 566, 696, 783, 797, 988, 1130, 1364
BshNI GGYRCC 2 cut(s) 711, 2272
BshVI ATCGAT 1 cut(s) 2220
BsiHKAI GWGCWC 4 cut(s) 318, 666, 1799, 2013
BsiSI CCGG 9 cut(s) 165, 259, 291, 300, 354, 1049, 1076, 1121, 2027
BslFI GGGAC 3 cut(s) 101, 1438, 1520
BslI CCNNNNNNNGG 8 cut(s) 211, 265, 573, 673, 703, 1081, 1357, 2146
BsmAI GTCTC 3 cut(s) 314, 1050, 1397
BsmBI CGTCTC 1 cut(s) 1397
BsmFI GGGAC 3 cut(s) 101, 1438, 1520
BsmI GAATGC 1 cut(s) 647
BsnI GGCC 9 cut(s) 96, 278, 566, 696, 783, 797, 988, 1130, 1364
Bso31I GGTCTC 1 cut(s) 314
Bsp120I GGGCCC 1 cut(s) 564
Bsp1286I GDGCHC 8 cut(s) 198, 318, 360, 568, 666, 716, 1799, 2013
Bsp1407I TGTACA 2 cut(s) 501, 1929
Bsp143I GATC 4 cut(s) 74, 237, 2052, 2217
Bsp19I CCATGG 1 cut(s) 2276
BspACI CCGC 8 cut(s) 205, 459, 516, 1057, 1079, 1236, 1785, 2097
BspANI GGCC 9 cut(s) 96, 278, 566, 696, 783, 797, 988, 1130, 1364
BspCNI CTCAG 4 cut(s) 226, 785, 1393, 1528
BspDI ATCGAT 1 cut(s) 2220
BspHI TCATGA 1 cut(s) 246
BspMI ACCTGC 1 cut(s) 1409
BspOI GCTAGC 2 cut(s) 1323, 1860
BspPI GGATC 3 cut(s) 69, 82, 2047
BspT107I GGYRCC 2 cut(s) 711, 2272
BspTI CTTAAG 1 cut(s) 1619
BspTNI GGTCTC 1 cut(s) 314
BsrDI GCAATG 3 cut(s) 555, 1312, 1342
BsrFI RCCGGY 1 cut(s) 2026
BsrGI TGTACA 2 cut(s) 501, 1929
BsrI ACTGG 3 cut(s) 729, 1759, 2098
BssAI RCCGGY 1 cut(s) 2026
BssECI CCNNGG 8 cut(s) 175, 197, 989, 1004, 1224, 1351, 1372, 2276
BssMI GATC 4 cut(s) 74, 237, 2052, 2217
BssNI GRCGYC 2 cut(s) 186, 878
BssSI CACGAG 1 cut(s) 317
BssT1I CCWWGG 3 cut(s) 175, 1372, 2276
Bst2BI CACGAG 1 cut(s) 317
Bst2UI CCWGG 9 cut(s) 199, 698, 720, 799, 990, 1005, 1628, 1807, 2270
Bst4CI ACNGT 3 cut(s) 604, 1460, 2020
Bst6I CTCTTC 1 cut(s) 1011
BstACI GRCGYC 2 cut(s) 186, 878
BstAFI CTTAAG 1 cut(s) 1619
BstAPI GCANNNNNTGC 1 cut(s) 1277
BstAUI TGTACA 2 cut(s) 501, 1929
BstBAI YACGTR 5 cut(s) 211, 439, 470, 639, 1450
BstC8I GCNNGC 7 cut(s) 830, 957, 1251, 1321, 1325, 1416, 1858
BstDEI CTNAG 8 cut(s) 18, 234, 772, 1038, 1401, 1536, 2141, 2282
BstDSI CCRYGG 1 cut(s) 2276
BstENI CCTNNNNNAGG 1 cut(s) 701
BstF5I GGATG 4 cut(s) 649, 1014, 1032, 1796
BstHHI GCGC 3 cut(s) 479, 961, 1535
BstKTI GATC 4 cut(s) 77, 240, 2055, 2220
BstMAI GTCTC 3 cut(s) 314, 1050, 1397
BstMBI GATC 4 cut(s) 74, 237, 2052, 2217
BstNI CCWGG 9 cut(s) 199, 698, 720, 799, 990, 1005, 1628, 1807, 2270
BstNSI RCATGY 7 cut(s) 508, 944, 959, 1327, 1513, 1936, 2086
BstPAI GACNNNNGTC 1 cut(s) 470
BstSLI GKGCMC 3 cut(s) 568, 666, 716
BstV1I GCAGC 5 cut(s) 155, 482, 1339, 1534, 1975
BstV2I GAAGAC 2 cut(s) 1704, 2133
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
Bsu15I ATCGAT 1 cut(s) 2220
BsuRI GGCC 9 cut(s) 96, 278, 566, 696, 783, 797, 988, 1130, 1364
BsuTUI ATCGAT 1 cut(s) 2220
BtgI CCRYGG 1 cut(s) 2276
BtgZI GCGATG 1 cut(s) 1669
BtrI CACGTC 1 cut(s) 48
BtsCI GGATG 4 cut(s) 649, 1014, 1032, 1796
BtsIMutI CAGTG 6 cut(s) 108, 231, 780, 1271, 1752, 2091
BveI ACCTGC 1 cut(s) 1409
Cac8I GCNNGC 7 cut(s) 830, 957, 1251, 1321, 1325, 1416, 1858
CaiI CAGNNNCTG 1 cut(s) 495
CciI TCATGA 1 cut(s) 246
CfoI GCGC 3 cut(s) 479, 961, 1535
Cfr10I RCCGGY 1 cut(s) 2026
ClaI ATCGAT 1 cut(s) 2220
CseI GACGC 2 cut(s) 194, 867
CsiI ACCWGGT 1 cut(s) 1626
Csp6I GTAC 8 cut(s) 288, 502, 605, 636, 1348, 1649, 1930, 2311
CspCI CAANNNNNGTGG 4 cut(s) 537, 572, 597, 632
CviQI GTAC 8 cut(s) 288, 502, 605, 636, 1348, 1649, 1930, 2311
DdeI CTNAG 8 cut(s) 18, 234, 772, 1038, 1401, 1536, 2141, 2282
DpnI GATC 4 cut(s) 76, 239, 2054, 2219
DpnII GATC 4 cut(s) 74, 237, 2052, 2217
DraI TTTAAA 1 cut(s) 844
DraIII CACNNNGTG 1 cut(s) 1453
EaeI YGGCCR 2 cut(s) 94, 795
Eam1104I CTCTTC 1 cut(s) 1011
EarI CTCTTC 1 cut(s) 1011
EciI GGCGGA 1 cut(s) 1800
Ecl136II GAGCTC 1 cut(s) 2011
Eco130I CCWWGG 3 cut(s) 175, 1372, 2276
Eco147I AGGCCT 1 cut(s) 988
Eco24I GRGCYC 4 cut(s) 198, 360, 568, 2013
Eco31I GGTCTC 1 cut(s) 314
Eco32I GATATC 1 cut(s) 804
Eco47I GGWCC 6 cut(s) 214, 392, 727, 1072, 1571, 1644
Eco53kI GAGCTC 1 cut(s) 2011
Eco57I CTGAAG 6 cut(s) 132, 382, 472, 1148, 1973, 2178
Eco72I CACGTG 3 cut(s) 211, 470, 1450
EcoICRI GAGCTC 1 cut(s) 2011
EcoNI CCTNNNNNAGG 1 cut(s) 701
EcoO109I RGGNCCY 4 cut(s) 392, 564, 694, 1072
EcoRII CCWGG 9 cut(s) 197, 696, 718, 797, 988, 1003, 1626, 1805, 2268
EcoRV GATATC 1 cut(s) 804
EcoT14I CCWWGG 3 cut(s) 175, 1372, 2276
EcoT22I ATGCAT 1 cut(s) 649
EcoT38I GRGCYC 4 cut(s) 198, 360, 568, 2013
ErhI CCWWGG 3 cut(s) 175, 1372, 2276
Esp3I CGTCTC 1 cut(s) 1397
FalI AAGNNNNNCTT 6 cut(s) 1592, 1624, 2124, 2156, 2284, 2316
FaqI GGGAC 3 cut(s) 101, 1438, 1520
Fnu4HI GCNGC 7 cut(s) 169, 205, 460, 496, 1328, 1548, 1964
FokI GGATG 4 cut(s) 656, 1021, 1039, 1803
FriOI GRGCYC 4 cut(s) 198, 360, 568, 2013
Fsp4HI GCNGC 7 cut(s) 169, 205, 460, 496, 1328, 1548, 1964
FspBI CTAG 4 cut(s) 590, 1320, 1680, 1857
GlaI GCGC 3 cut(s) 478, 960, 1534
GluI GCNGC 7 cut(s) 169, 205, 460, 496, 1328, 1548, 1964
GsaI CCCAGC 1 cut(s) 813
GsuI CTGGAG 3 cut(s) 702, 1587, 1776
HaeIII GGCC 9 cut(s) 96, 278, 566, 696, 783, 797, 988, 1130, 1364
HapII CCGG 9 cut(s) 165, 259, 291, 300, 354, 1049, 1076, 1121, 2027
HgaI GACGC 2 cut(s) 194, 867
HhaI GCGC 3 cut(s) 479, 961, 1535
Hin1I GRCGYC 2 cut(s) 186, 878
Hin6I GCGC 3 cut(s) 477, 959, 1533
HinP1I GCGC 3 cut(s) 477, 959, 1533
HincII GTYRAC 2 cut(s) 2080, 2298
HindII GTYRAC 2 cut(s) 2080, 2298
HindIII AAGCTT 1 cut(s) 1613
HpaII CCGG 9 cut(s) 165, 259, 291, 300, 354, 1049, 1076, 1121, 2027
HphI GGTGA 8 cut(s) 42, 135, 530, 571, 1465, 2008, 2041, 2161
Hpy188I TCNGA 8 cut(s) 224, 401, 1225, 1355, 1402, 1972, 2067, 2197
Hpy188III TCNNGA 7 cut(s) 247, 386, 490, 761, 1554, 1566, 1729
Hpy99I CGWCG 2 cut(s) 188, 194
HpyCH4III ACNGT 3 cut(s) 604, 1460, 2020
HpyCH4IV ACGT 9 cut(s) 27, 47, 210, 366, 438, 469, 638, 1449, 2247
HpyF3I CTNAG 8 cut(s) 18, 234, 772, 1038, 1401, 1536, 2141, 2282
HpySE526I ACGT 9 cut(s) 27, 47, 210, 366, 438, 469, 638, 1449, 2247
Hsp92I GRCGYC 2 cut(s) 186, 878
HspAI GCGC 3 cut(s) 477, 959, 1533
Kzo9I GATC 4 cut(s) 74, 237, 2052, 2217
LmnI GCTCC 8 cut(s) 201, 313, 363, 492, 591, 937, 1411, 2016
Lsp1109I GCAGC 5 cut(s) 155, 482, 1339, 1534, 1975
LweI GCATC 6 cut(s) 321, 488, 634, 999, 1559, 1646
MabI ACCWGGT 1 cut(s) 1626
MaeI CTAG 4 cut(s) 590, 1320, 1680, 1857
MaeII ACGT 9 cut(s) 27, 47, 210, 366, 438, 469, 638, 1449, 2247
MaeIII GTNAC 6 cut(s) 48, 141, 1445, 1586, 1811, 1843
MalI GATC 4 cut(s) 76, 239, 2054, 2219
MboI GATC 4 cut(s) 74, 237, 2052, 2217
MfeI CAATTG 2 cut(s) 2103, 2205
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 8 cut(s) 198, 318, 360, 568, 666, 716, 1799, 2013
MlsI TGGCCA 2 cut(s) 96, 797
MluNI TGGCCA 2 cut(s) 96, 797
MlyI GAGTC 5 cut(s) 344, 427, 764, 1583, 2117
MmeI TCCRAC 4 cut(s) 102, 436, 1363, 1576
Mox20I TGGCCA 2 cut(s) 96, 797
Mph1103I ATGCAT 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 1295
MscI TGGCCA 2 cut(s) 96, 797
MseI TTAA 8 cut(s) 24, 150, 843, 861, 996, 1620, 1773, 2088
MslI CAYNNNNRTG 5 cut(s) 449, 945, 1299, 1335, 1528
Msp20I TGGCCA 2 cut(s) 96, 797
MspA1I CMGCKG 1 cut(s) 809
MspCI CTTAAG 1 cut(s) 1619
MspI CCGG 9 cut(s) 165, 259, 291, 300, 354, 1049, 1076, 1121, 2027
MunI CAATTG 2 cut(s) 2103, 2205
Mva1269I GAATGC 1 cut(s) 647
MvaI CCWGG 9 cut(s) 199, 698, 720, 799, 990, 1005, 1628, 1807, 2270
NciI CCSGG 3 cut(s) 355, 1050, 1076
NcoI CCATGG 1 cut(s) 2276
NdeII GATC 4 cut(s) 74, 237, 2052, 2217
NheI GCTAGC 2 cut(s) 1319, 1856
NmuCI GTSAC 5 cut(s) 48, 141, 1445, 1586, 1843
NsiI ATGCAT 1 cut(s) 649
NspI RCATGY 7 cut(s) 508, 944, 959, 1327, 1513, 1936, 2086
OliI CACNNNNGTG 1 cut(s) 1528
PaeI GCATGC 2 cut(s) 959, 1327
PagI TCATGA 1 cut(s) 246
PaqCI CACCTGC 1 cut(s) 1409
PceI AGGCCT 1 cut(s) 988
PciI ACATGT 2 cut(s) 1509, 1932
PcsI WCGNNNNNNNCGW 3 cut(s) 189, 435, 1526
PctI GAATGC 1 cut(s) 647
PdmI GAANNNNTTC 1 cut(s) 1295
PfeI GAWTC 5 cut(s) 373, 1150, 1879, 1891, 2135
PfoI TCCNGGA 2 cut(s) 718, 1048
PkrI GCNGC 7 cut(s) 170, 206, 461, 497, 1329, 1549, 1965
PleI GAGTC 5 cut(s) 344, 426, 764, 1583, 2116
PmaCI CACGTG 3 cut(s) 211, 470, 1450
PmlI CACGTG 3 cut(s) 211, 470, 1450
PpsI GAGTC 5 cut(s) 344, 426, 764, 1583, 2116
Ppu21I YACGTR 5 cut(s) 211, 439, 470, 639, 1450
PpuMI RGGWCCY 2 cut(s) 392, 1072
PscI ACATGT 2 cut(s) 1509, 1932
PshAI GACNNNNGTC 1 cut(s) 470
PshBI ATTAAT 1 cut(s) 996
PsiI TTATAA 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 2013
Psp5II RGGWCCY 2 cut(s) 392, 1072
Psp6I CCWGG 9 cut(s) 197, 696, 718, 797, 988, 1003, 1626, 1805, 2268
PspCI CACGTG 3 cut(s) 211, 470, 1450
PspFI CCCAGC 1 cut(s) 809
PspGI CCWGG 9 cut(s) 197, 696, 718, 797, 988, 1003, 1626, 1805, 2268
PspOMI GGGCCC 1 cut(s) 564
PspPPI RGGWCCY 2 cut(s) 392, 1072
PsrI GAACNNNNNNTAC 2 cut(s) 1448, 1480
PstNI CAGNNNCTG 1 cut(s) 495
PsuI RGATCY 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 8 cut(s) 289, 503, 606, 637, 1349, 1650, 1931, 2312
RsaNI GTAC 8 cut(s) 288, 502, 605, 636, 1348, 1649, 1930, 2311
RseI CAYNNNNRTG 5 cut(s) 449, 945, 1299, 1335, 1528
SacI GAGCTC 1 cut(s) 2013
SaqAI TTAA 8 cut(s) 24, 150, 843, 861, 996, 1620, 1773, 2088
SatI GCNGC 7 cut(s) 169, 205, 460, 496, 1328, 1548, 1964
Sau3AI GATC 4 cut(s) 74, 237, 2052, 2217
SchI GAGTC 5 cut(s) 344, 427, 764, 1583, 2117
SduI GDGCHC 8 cut(s) 198, 318, 360, 568, 666, 716, 1799, 2013
SexAI ACCWGGT 1 cut(s) 1626
SfaNI GCATC 6 cut(s) 321, 488, 634, 999, 1559, 1646
SgrAI CRCCGGYG 1 cut(s) 2026
SinI GGWCC 6 cut(s) 214, 392, 727, 1072, 1571, 1644
SmiMI CAYNNNNRTG 5 cut(s) 449, 945, 1299, 1335, 1528
SmlI CTYRAG 3 cut(s) 1211, 1619, 1727
SmoI CTYRAG 3 cut(s) 1211, 1619, 1727
SphI GCATGC 2 cut(s) 959, 1327
SseBI AGGCCT 1 cut(s) 988
SsiI CCGC 8 cut(s) 205, 459, 516, 1057, 1079, 1236, 1785, 2097
SspMI CTAG 4 cut(s) 590, 1320, 1680, 1857
SstI GAGCTC 1 cut(s) 2013
StuI AGGCCT 1 cut(s) 988
StyI CCWWGG 3 cut(s) 175, 1372, 2276
TaaI ACNGT 3 cut(s) 604, 1460, 2020
TaiI ACGT 9 cut(s) 30, 50, 213, 369, 441, 472, 641, 1452, 2250
TaqI TCGA 7 cut(s) 183, 464, 482, 1520, 1872, 1992, 2220
TatI WGTACW 2 cut(s) 501, 1929
TauI GCSGC 2 cut(s) 207, 462
TfiI GAWTC 5 cut(s) 373, 1150, 1879, 1891, 2135
Tru1I TTAA 8 cut(s) 24, 150, 843, 861, 996, 1620, 1773, 2088
Tru9I TTAA 8 cut(s) 24, 150, 843, 861, 996, 1620, 1773, 2088
TscAI CASTG 6 cut(s) 115, 238, 780, 1278, 1759, 2098
TseFI GTSAC 5 cut(s) 48, 141, 1445, 1586, 1843
TseI GCWGC 5 cut(s) 168, 495, 1327, 1547, 1963
Tsp45I GTSAC 5 cut(s) 48, 141, 1445, 1586, 1843
TspDTI ATGAA 3 cut(s) 235, 566, 2231
TspGWI ACGGA 2 cut(s) 1373, 1636
TspRI CASTG 6 cut(s) 115, 238, 780, 1278, 1759, 2098
Vha464I CTTAAG 1 cut(s) 1619
VneI GTGCAC 1 cut(s) 662
VpaK11BI GGWCC 6 cut(s) 214, 392, 727, 1072, 1571, 1644
VspI ATTAAT 1 cut(s) 996
XagI CCTNNNNNAGG 1 cut(s) 701
XapI RAATTY 3 cut(s) 857, 923, 2117
XceI RCATGY 7 cut(s) 508, 944, 959, 1327, 1513, 1936, 2086
XmnI GAANNNNTTC 1 cut(s) 1295
XspI CTAG 4 cut(s) 590, 1320, 1680, 1857
Zsp2I ATGCAT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.