Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0005392

Physical Location & Seq

Genomic Coordinates Reverse (-)

81386536 .. 81389323

Gene Structure 2788 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022154

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGGCCCCAAATTTAAGCAATGGCTTTGCCGTCGTTAACGTCGACAATGGCGCAAACCCAAGTCCTTTTAGCCTTCAAGGTTCCAACTTGACGGCCAACGGCCACGTCATCCTCTCCGACGTCCCCCAAAACATAACACTCGGCCCATCACCCCACACCATCAACAAGTCCACCGCCCCCGGCTCTTTTGTTGGCTTCGAAGCCGCCGAGGCCAGAAGCCGCCATGTGGTGCCCGTCGGCCAGCTGAAGGACATCCGGTTCATGAGCATCTTCCGGTTCAAGGTCTGGTGGACCACTCACTGGGTCGGATCCAACGGCCGTGACCTCGAGCAGGAGACCCAGATCGTCATCCTCGAAAACTCCGACGCCGGCAGGCCCTACGTCGTCATCCTCCCTCTCCTTGAAGGCGGCTTCCGAACTTCCATCCAGCCAGGCGCCGAGGACTACCTCGACGTCTGTGTAGAGAGCGGTTCAACGCAGGTCAATGAAAAGGGATTCTCCAGCGTGCTTTATCTTCACGCCGGAGAAGATCCCTTCACGCTGGTGAAGGACGCCGTCAAGGTCGCCAAGGCGCACTTAGGAACATTCAATCTGTTGGAGGAGAAGACGCCACCTGGCATCGTCGACAAGTTCGGTTGGTGCACATGGGATGCTTTTTACCTCACTGTGCACCCTCGCGGCGTCATTGATGGCGTGAGGAAGCTTGTGGAGGGTGGGTGCCCTCCAGGGCTTGTGTTGCTGGACGACGGGTGGCAGTCCATCGGCCACGACTCTGATCCCATCACCAAAGAAGGGATGGGCCAGACCGCCGCCGGTGAGCAAATGCCGTGCAGGCTGCTCAAGTTCAAAGAGAACTACAAGTTCATAGACTATGTCAGCTCCAAGGACCCTCGTAGCCAAGGCATGGGTGCGTTTGTGAAGGACTTGAAGGACGAGTTCAAGAGCGTTGACTACGTGTACGTGTGGCACGCGTTGTGTGGGTACTGGGGTGGGATCCGTCCCAATGTTCCTGGCCTGCCGGAGGCGGTGGTTGTTAAGCCGGAACTGTCACCGGGGTTGAAGAACACGATGGAAGATCTGGCCGTGGATAAGATAGTCAACACTGGGATTGGGCTGGTCCCACCGGAAATGGTTAACCAGATGTATGAAGGGCTCCACTCTCACCTGAAGTCCGTCGGGATCGACGGCGTCAAGGTCGACGTTATCCATTTGTTGGAAATGTTGTGTGAGAACTATGGTGGCCGAGTGAACCTGGCTAAGGCATATTACGATGCCCTGACAGCTTCAGTGAGGAAGCACTTCGACGGCAATGGTGTCATTGCTAGCATGGAGCACTGTAACGACTTCATGTTCCTTGGAACCGAAGCCATCACTCTTGGCCGTGTCGGTGACGATTTCTGGTGCACTGACCCCTCTGGTGATCCCAACGGTACCTTTTGGTTGCAAGGGTGTCACATGGTTCATTGTGCTTACAATAGCTTATGGATGGGTAACTTCATACAGCCGGATTGGGACATGTTCCAGTCCACTCATCCCTGTGCCGCTTTCCATGCTGCCTCTAGGGCAATCTCCGGGGGTCCTATTTATGTGAGTGACACTGTGGGGAAGCACAACTTTGACTTGCTCAAAACCCTAGTGGTGCCTGACGGCTCTATCCTTCGCTGCGAGTACTATGCTCTCCCTACCCGCGATTGTCTTTTTGAGGATCCTCTCCATGATGGCCGCACCATGCTCAAAATTTGGAACCTCAACAAGTTCAATGGAGTTCTTGGAGCCTTTAACTGCCAAGGAGGAGGCTGGAGCCGTGAGACTAGGCGAAACCAATGTTTTTCTAAATTCGTGGGAAAGGTGACCTCCAAGGCCAACCCTAAAGACATTGAGTGGAACAGTGGCAAGAGTCCAATTTCCATTGAAGGAGTGCAACTGTTTGCCTTGTACTATCACCAGGCCAAGAAGCTAGTCCTGTCTAAGCCAACCGAGGATGTGGAGTTATCACTGGAGCCCTTCAACTTTGAGCTAATTGTCGTTTCTCCAGTGACTACTTTGCCTAGCAAGTCTGTCCAATTTGCCCCTATTGGCTTAGTGAACATGCTCAACACCGGCGGTGCTGTCAAGTCGTTGGTTTACAGTGAGGAGGGAAACTCTGTTCAAATTGGGGTGAAGGGCACCGGAGAGATGAGGGTGTTTGCGTCTGAGAAGCCACTGGGTTGCAATATTGATGGAAGCTGTGTCGCTTTTGAGTATGAAGAGAACATGGTTAGAGTTCATGTGCCATGGACTGGTACCTCCGCCTTGTCTATTGTGGAGTACATATTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010035 GO:0016740 GO:0016757 GO:0016758 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0047274 GO:0050896 GO:0097305 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00052 map00052

Pfam Domains

Protein Families

Raffinose_syn

Protein Analysis

773

Amino Acids

84.74

Weight (kDa)

5.52

Isoelectric Point (pI)

30.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Raffinose_syn	PF05691	24 - 755	0.0	Raffinose synthase or seed imbibition protein Sip1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005392)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40390
fragaria_vesca	FvH4_5g05860 FvH4_6g51380
malus_domestica	MD06G1169800.v1.1 MD14G1175300.v1.1 MD17G1030100.v1.1
prunus_persica	Prupe.3G289900_v2.0.a1
pyrus_communis	pycom06g15120 pycom14g14630 pycom17g02750
rosa_chinensis	RchiOBHm_Chr2g0173061 RchiOBHm_Chr7g0192961
rosa_laevigata	RLG00000004308 RLG00000022154
rosa_multiflora	Rmu_sc0002040.1_g000062 Rmu_sc0011236.1_g000005
rosa_roxburghii	Rroxscaffold_2G00079280 Rroxscaffold_3G00262500
rosa_rugosa	Rorug02G0564900 Rorug07G0006500
rosa_samantha	Rh2AG644700 Rh2BG655500 Rh2CG620600 Rh2DG668800 Rh7AG131800 Rh7BG132600 Rh7DG133700
rosa_wichuraiana	Rw2G052950 Rw7G011240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	123, 456
Acc36I	ACCTGC	1 cut(s)	469
Acc65I	GGTACC	2 cut(s)	1430, 2282
AccB1I	GGYRCC	7 cut(s)	229, 434, 717, 1430, 1639, 2165, 2282
AccB7I	CCANNNNNTGG	4 cut(s)	300, 904, 1213, 2279
AccBSI	CCGCTC	1 cut(s)	468
AccI	GTMKAC	3 cut(s)	42, 624, 1197
AccII	CGCG	3 cut(s)	678, 971, 1689
AcoI	YGGCCR	9 cut(s)	93, 100, 238, 316, 763, 1080, 1240, 1378, 1720
AcsI	RAATTY	3 cut(s)	10, 1737, 1835
AcuI	CTGAAG	3 cut(s)	266, 1187, 1269
AcyI	GRCGYC	8 cut(s)	120, 366, 435, 453, 552, 608, 681, 1188
AfaI	GTAC	7 cut(s)	959, 983, 1432, 1670, 1937, 2284, 2309
AflIII	ACRYGT	4 cut(s)	954, 960, 969, 1515
AjiI	CACGTC	1 cut(s)	106
AjnI	CCWGG	6 cut(s)	430, 613, 724, 1009, 1251, 1944
AleI	CACNNNNGTG	1 cut(s)	542
AloI	GAACNNNNNNTCC	4 cut(s)	242, 274, 2130, 2162
AluBI	AGCT	8 cut(s)	244, 703, 879, 1283, 1479, 1957, 2017, 2226
AluI	AGCT	8 cut(s)	244, 703, 879, 1283, 1479, 1957, 2017, 2226
Alw21I	GWGCWC	4 cut(s)	644, 672, 1335, 1406
Alw26I	GTCTC	2 cut(s)	329, 1802
Alw44I	GTGCAC	3 cut(s)	640, 668, 1402
Ama87I	CYCGRG	1 cut(s)	326
ApaLI	GTGCAC	3 cut(s)	640, 668, 1402
ApeKI	GCWGC	3 cut(s)	835, 1553, 1662
ApoI	RAATTY	3 cut(s)	10, 1737, 1835
Asp700I	GAANNNNTTC	1 cut(s)	1298
Asp718I	GGTACC	2 cut(s)	1430, 2282
AspLEI	GCGC	3 cut(s)	53, 437, 574
AspS9I	GGNCC	8 cut(s)	4, 143, 291, 375, 799, 886, 1117, 1577
AsuC2I	CCSGG	3 cut(s)	180, 1053, 1573
AsuII	TTCGAA	1 cut(s)	198
AsuNHI	GCTAGC	1 cut(s)	1322
AvaI	CYCGRG	1 cut(s)	326
AvaII	GGWCC	4 cut(s)	291, 886, 1117, 1577
BaeGI	GKGCMC	6 cut(s)	234, 644, 672, 722, 1406, 2168
BaeI	ACNNNNGTAYC	2 cut(s)	973, 1006
BamHI	GGATCC	3 cut(s)	308, 993, 1705
BanI	GGYRCC	7 cut(s)	229, 434, 717, 1430, 1639, 2165, 2282
BanII	GRGCYC	2 cut(s)	1155, 2004
BbsI	GAAGAC	1 cut(s)	611
Bbv12I	GWGCWC	4 cut(s)	644, 672, 1335, 1406
BbvI	GCAGC	3 cut(s)	822, 1540, 1649
BcgI	CGANNNNNNTGC	2 cut(s)	1655, 1689
BciT130I	CCWGG	6 cut(s)	432, 615, 726, 1011, 1253, 1946
BcnI	CCSGG	3 cut(s)	180, 1053, 1573
BcoDI	GTCTC	2 cut(s)	329, 1802
BfaI	CTAG	7 cut(s)	1323, 1560, 1634, 1812, 1958, 2049, 2317
BfoI	RGCGCY	1 cut(s)	438
BfuAI	ACCTGC	1 cut(s)	469
BglI	GCCNNNNNGGC	3 cut(s)	209, 832, 1562
BglII	AGATCT	1 cut(s)	1075
BmcAI	AGTACT	1 cut(s)	1670
Bme1390I	CCNGG	9 cut(s)	180, 432, 615, 726, 1011, 1053, 1253, 1573, 1946
Bme18I	GGWCC	4 cut(s)	291, 886, 1117, 1577
BmeT110I	CYCGRG	1 cut(s)	326
BmgBI	CACGTC	1 cut(s)	106
BmgT120I	GGNCC	8 cut(s)	4, 143, 291, 375, 799, 886, 1117, 1577
BmrFI	CCNGG	9 cut(s)	180, 432, 615, 726, 1011, 1053, 1253, 1573, 1946
BmrI	ACTGGG	4 cut(s)	310, 994, 1113, 2213
BmsI	GCATC	4 cut(s)	276, 627, 640, 1261
BmtI	GCTAGC	1 cut(s)	1326
BmuI	ACTGGG	4 cut(s)	310, 994, 1113, 2213
BpiI	GAAGAC	1 cut(s)	611
BplI	GAGNNNNNCTC	2 cut(s)	2126, 2158
BpmI	CTGGAG	5 cut(s)	484, 708, 1819, 2016, 2018
Bpu10I	CCTNAGC	1 cut(s)	1257
Bpu14I	TTCGAA	1 cut(s)	198
BpuEI	CTTGAG	1 cut(s)	824
BpuMI	CCSGG	3 cut(s)	180, 1053, 1573
BsaAI	YACGTR	2 cut(s)	955, 961
BsaBI	GATNNNNATC	1 cut(s)	347
BsaHI	GRCGYC	8 cut(s)	120, 366, 435, 453, 552, 608, 681, 1188
BsaI	GGTCTC	1 cut(s)	329
BsaWI	WCCGGW	4 cut(s)	255, 273, 1123, 2168
BsaXI	ACNNNNNCTCC	2 cut(s)	701, 731
Bse118I	RCCGGY	3 cut(s)	368, 812, 2099
Bse1I	ACTGG	8 cut(s)	305, 989, 1108, 1522, 2001, 2033, 2208, 2284
Bse3DI	GCAATG	3 cut(s)	25, 1315, 1317
Bse8I	GATNNNNATC	1 cut(s)	347
BseBI	CCWGG	6 cut(s)	432, 615, 726, 1011, 1253, 1946
BseJI	GATNNNNATC	1 cut(s)	347
BseMI	GCAATG	3 cut(s)	25, 1315, 1317
BseMII	CTCAG	1 cut(s)	2184
BseNI	ACTGG	8 cut(s)	305, 989, 1108, 1522, 2001, 2033, 2208, 2284
BseRI	GAGGAG	3 cut(s)	614, 1806, 2147
BseSI	GKGCMC	6 cut(s)	234, 644, 672, 722, 1406, 2168
BseX3I	CGGCCG	1 cut(s)	316
BseXI	GCAGC	3 cut(s)	822, 1540, 1649
BsgI	GTGCAG	1 cut(s)	850
Bsh1236I	CGCG	3 cut(s)	678, 971, 1689
Bsh1285I	CGRYCG	1 cut(s)	319
BshNI	GGYRCC	7 cut(s)	229, 434, 717, 1430, 1639, 2165, 2282
BsiEI	CGRYCG	1 cut(s)	319
BsiHKAI	GWGCWC	4 cut(s)	644, 672, 1335, 1406
BsiHKCI	CYCGRG	1 cut(s)	326
BslFI	GGGAC	4 cut(s)	107, 984, 1103, 1526
BsmAI	GTCTC	2 cut(s)	329, 1802
BsmFI	GGGAC	4 cut(s)	107, 984, 1103, 1526
Bso31I	GGTCTC	1 cut(s)	329
BsoBI	CYCGRG	1 cut(s)	326
Bsp119I	TTCGAA	1 cut(s)	198
Bsp1286I	GDGCHC	9 cut(s)	234, 644, 672, 722, 1155, 1335, 1406, 2004, 2168
Bsp143I	GATC	9 cut(s)	308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
Bsp19I	CCATGG	1 cut(s)	2273
BspCNI	CTCAG	1 cut(s)	2185
BspFNI	CGCG	3 cut(s)	678, 971, 1689
BspHI	TCATGA	1 cut(s)	261
BspMI	ACCTGC	1 cut(s)	469
BspOI	GCTAGC	1 cut(s)	1326
BspT104I	TTCGAA	1 cut(s)	198
BspT107I	GGYRCC	7 cut(s)	229, 434, 717, 1430, 1639, 2165, 2282
BspTNI	GGTCTC	1 cut(s)	329
BsrBI	CCGCTC	1 cut(s)	468
BsrDI	GCAATG	3 cut(s)	25, 1315, 1317
BsrFI	RCCGGY	3 cut(s)	368, 812, 2099
BsrI	ACTGG	8 cut(s)	305, 989, 1108, 1522, 2001, 2033, 2208, 2284
BssAI	RCCGGY	3 cut(s)	368, 812, 2099
BssMI	GATC	9 cut(s)	308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
BssNI	GRCGYC	8 cut(s)	120, 366, 435, 453, 552, 608, 681, 1188
BssT1I	CCWWGG	7 cut(s)	567, 882, 898, 1354, 1786, 1857, 2273
Bst2UI	CCWGG	6 cut(s)	432, 615, 726, 1011, 1253, 1946
Bst4CI	ACNGT	8 cut(s)	667, 1047, 1337, 1430, 1600, 1889, 1926, 2129
Bst6I	CTCTTC	1 cut(s)	2241
BstACI	GRCGYC	8 cut(s)	120, 366, 435, 453, 552, 608, 681, 1188
BstBAI	YACGTR	2 cut(s)	955, 961
BstBI	TTCGAA	1 cut(s)	198
BstC8I	GCNNGC	8 cut(s)	242, 370, 374, 506, 833, 969, 1016, 1324
BstDEI	CTNAG	5 cut(s)	577, 1257, 1968, 2080, 2193
BstDSI	CCRYGG	2 cut(s)	1083, 2273
BstEII	GGTNACC	1 cut(s)	1849
BstENI	CCTNNNNNAGG	3 cut(s)	329, 1019, 1256
BstFNI	CGCG	3 cut(s)	678, 971, 1689
BstH2I	RGCGCY	1 cut(s)	438
BstHHI	GCGC	3 cut(s)	53, 437, 574
BstKTI	GATC	9 cut(s)	311, 345, 532, 778, 996, 1078, 1182, 1423, 1708
BstMAI	GTCTC	2 cut(s)	329, 1802
BstMBI	GATC	9 cut(s)	308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
BstMCI	CGRYCG	1 cut(s)	319
BstMWI	GCNNNNNNNGC	6 cut(s)	209, 736, 832, 1280, 1550, 1562
BstNI	CCWGG	6 cut(s)	432, 615, 726, 1011, 1253, 1946
BstNSI	RCATGY	2 cut(s)	1519, 2092
BstPI	GGTNACC	1 cut(s)	1849
BstSCI	CCNGG	9 cut(s)	178, 430, 613, 724, 1009, 1051, 1251, 1571, 1944
BstSLI	GKGCMC	6 cut(s)	234, 644, 672, 722, 1406, 2168
BstUI	CGCG	3 cut(s)	678, 971, 1689
BstV1I	GCAGC	3 cut(s)	822, 1540, 1649
BstV2I	GAAGAC	1 cut(s)	611
BstX2I	RGATCY	5 cut(s)	308, 529, 993, 1075, 1705
BstYI	RGATCY	5 cut(s)	308, 529, 993, 1075, 1705
BstZI	CGGCCG	1 cut(s)	316
BtgI	CCRYGG	2 cut(s)	1083, 2273
BtrI	CACGTC	1 cut(s)	106
BveI	ACCTGC	1 cut(s)	469
Cac8I	GCNNGC	8 cut(s)	242, 370, 374, 506, 833, 969, 1016, 1324
CciI	TCATGA	1 cut(s)	261
CfoI	GCGC	3 cut(s)	53, 437, 574
Cfr10I	RCCGGY	3 cut(s)	368, 812, 2099
Cfr13I	GGNCC	8 cut(s)	4, 143, 291, 375, 799, 886, 1117, 1577
CseI	GACGC	6 cut(s)	374, 560, 616, 670, 1177, 2178
Csp6I	GTAC	7 cut(s)	958, 982, 1431, 1669, 1936, 2283, 2308
CviQI	GTAC	7 cut(s)	958, 982, 1431, 1669, 1936, 2283, 2308
DdeI	CTNAG	5 cut(s)	577, 1257, 1968, 2080, 2193
DinI	GGCGCC	1 cut(s)	436
DpnI	GATC	9 cut(s)	310, 344, 531, 777, 995, 1077, 1181, 1422, 1707
DpnII	GATC	9 cut(s)	308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
EaeI	YGGCCR	9 cut(s)	93, 100, 238, 316, 763, 1080, 1240, 1378, 1720
EagI	CGGCCG	1 cut(s)	316
Eam1104I	CTCTTC	1 cut(s)	2241
EarI	CTCTTC	1 cut(s)	2241
EciI	GGCGGA	1 cut(s)	2278
EclXI	CGGCCG	1 cut(s)	316
Eco130I	CCWWGG	7 cut(s)	567, 882, 898, 1354, 1786, 1857, 2273
Eco24I	GRGCYC	2 cut(s)	1155, 2004
Eco31I	GGTCTC	1 cut(s)	329
Eco47I	GGWCC	4 cut(s)	291, 886, 1117, 1577
Eco52I	CGGCCG	1 cut(s)	316
Eco57I	CTGAAG	3 cut(s)	266, 1187, 1269
Eco88I	CYCGRG	1 cut(s)	326
Eco91I	GGTNACC	1 cut(s)	1849
EcoNI	CCTNNNNNAGG	3 cut(s)	329, 1019, 1256
EcoO109I	RGGNCCY	3 cut(s)	375, 886, 1577
EcoO65I	GGTNACC	1 cut(s)	1849
EcoRII	CCWGG	6 cut(s)	430, 613, 724, 1009, 1251, 1944
EcoT14I	CCWWGG	7 cut(s)	567, 882, 898, 1354, 1786, 1857, 2273
EcoT38I	GRGCYC	2 cut(s)	1155, 2004
EgeI	GGCGCC	1 cut(s)	436
EheI	GGCGCC	1 cut(s)	436
ErhI	CCWWGG	7 cut(s)	567, 882, 898, 1354, 1786, 1857, 2273
FalI	AAGNNNNNCTT	4 cut(s)	560, 592, 1598, 1630
FaqI	GGGAC	4 cut(s)	107, 984, 1103, 1526
FauI	CCCGC	1 cut(s)	1694
FblI	GTMKAC	3 cut(s)	42, 624, 1197
FriOI	GRGCYC	2 cut(s)	1155, 2004
FspBI	CTAG	7 cut(s)	1323, 1560, 1634, 1812, 1958, 2049, 2317
GlaI	GCGC	3 cut(s)	52, 436, 573
GsuI	CTGGAG	5 cut(s)	484, 708, 1819, 2016, 2018
HaeII	RGCGCY	1 cut(s)	438
HgaI	GACGC	6 cut(s)	374, 560, 616, 670, 1177, 2178
HhaI	GCGC	3 cut(s)	53, 437, 574
Hin1I	GRCGYC	8 cut(s)	120, 366, 435, 453, 552, 608, 681, 1188
Hin6I	GCGC	3 cut(s)	51, 435, 572
HinP1I	GCGC	3 cut(s)	51, 435, 572
HincII	GTYRAC	7 cut(s)	37, 43, 625, 949, 1099, 1135, 1198
HindII	GTYRAC	7 cut(s)	37, 43, 625, 949, 1099, 1135, 1198
HindIII	AAGCTT	1 cut(s)	701
HinfI	GANTC	3 cut(s)	495, 770, 1897
HpaI	GTTAAC	2 cut(s)	37, 1135
Hpy188I	TCNGA	6 cut(s)	118, 308, 364, 416, 775, 2194
Hpy188III	TCNNGA	3 cut(s)	262, 940, 1177
HpyCH4III	ACNGT	8 cut(s)	667, 1047, 1337, 1430, 1600, 1889, 1926, 2129
HpyCH4IV	ACGT	8 cut(s)	39, 105, 120, 381, 453, 954, 960, 1200
HpyCH4V	TGCA	7 cut(s)	642, 670, 831, 1404, 1444, 1921, 2211
HpyF10VI	GCNNNNNNNGC	6 cut(s)	209, 736, 832, 1280, 1550, 1562
HpyF3I	CTNAG	5 cut(s)	577, 1257, 1968, 2080, 2193
HpySE526I	ACGT	8 cut(s)	39, 105, 120, 381, 453, 954, 960, 1200
Hsp92I	GRCGYC	8 cut(s)	120, 366, 435, 453, 552, 608, 681, 1188
HspAI	GCGC	3 cut(s)	51, 435, 572
KasI	GGCGCC	1 cut(s)	434
KpnI	GGTACC	2 cut(s)	1434, 2286
KroI	GCCGGC	1 cut(s)	368
KroNI	GCCGGC	1 cut(s)	370
KspAI	GTTAAC	2 cut(s)	37, 1135
Kzo9I	GATC	9 cut(s)	308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
LmnI	GCTCC	6 cut(s)	884, 1158, 1330, 1772, 1800, 1999
Lsp1109I	GCAGC	3 cut(s)	822, 1540, 1649
LweI	GCATC	4 cut(s)	276, 627, 640, 1261
MaeI	CTAG	7 cut(s)	1323, 1560, 1634, 1812, 1958, 2049, 2317
MaeII	ACGT	8 cut(s)	39, 105, 120, 381, 453, 954, 960, 1200
MaeIII	GTNAC	9 cut(s)	320, 1047, 1337, 1388, 1451, 1490, 1592, 1849, 2035
MalI	GATC	9 cut(s)	310, 344, 531, 777, 995, 1077, 1181, 1422, 1707
MbiI	CCGCTC	1 cut(s)	468
MboI	GATC	9 cut(s)	308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
MboII	GAAGA	7 cut(s)	262, 506, 539, 616, 1072, 1085, 2258
MflI	RGATCY	5 cut(s)	308, 529, 993, 1075, 1705
MhlI	GDGCHC	9 cut(s)	234, 644, 672, 722, 1155, 1335, 1406, 2004, 2168
MluCI	AATT	7 cut(s)	10, 1737, 1835, 1902, 2019, 2063, 2151
MluI	ACGCGT	1 cut(s)	969
Mly113I	GGCGCC	1 cut(s)	435
MlyI	GAGTC	2 cut(s)	764, 1906
MmeI	TCCRAC	7 cut(s)	108, 141, 286, 336, 387, 576, 1194
MroNI	GCCGGC	1 cut(s)	368
MroXI	GAANNNNTTC	1 cut(s)	1298
MseI	TTAA	5 cut(s)	14, 36, 1035, 1134, 1779
MslI	CAYNNNNRTG	2 cut(s)	542, 1536
MspA1I	CMGCKG	1 cut(s)	244
MspR9I	CCNGG	9 cut(s)	180, 432, 615, 726, 1011, 1053, 1253, 1573, 1946
MvaI	CCWGG	6 cut(s)	432, 615, 726, 1011, 1253, 1946
MvnI	CGCG	3 cut(s)	678, 971, 1689
MwoI	GCNNNNNNNGC	6 cut(s)	209, 736, 832, 1280, 1550, 1562
NaeI	GCCGGC	1 cut(s)	370
NarI	GGCGCC	1 cut(s)	435
NciI	CCSGG	3 cut(s)	180, 1053, 1573
NcoI	CCATGG	1 cut(s)	2273
NdeII	GATC	9 cut(s)	308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
NgoMIV	GCCGGC	1 cut(s)	368
NheI	GCTAGC	1 cut(s)	1322
NmeAIII	GCCGAG	4 cut(s)	120, 232, 463, 1268
NmuCI	GTSAC	7 cut(s)	320, 1047, 1388, 1451, 1592, 1849, 2035
NspI	RCATGY	2 cut(s)	1519, 2092
NspV	TTCGAA	1 cut(s)	198
OliI	CACNNNNGTG	1 cut(s)	542
PaeR7I	CTCGAG	1 cut(s)	326
PagI	TCATGA	1 cut(s)	261
PciI	ACATGT	1 cut(s)	1515
PcsI	WCGNNNNNNNCGW	4 cut(s)	39, 204, 351, 360
PdiI	GCCGGC	1 cut(s)	370
PdmI	GAANNNNTTC	1 cut(s)	1298
PfeI	GAWTC	1 cut(s)	495
PflFI	GACNNNGTC	3 cut(s)	554, 872, 1187
PflMI	CCANNNNNTGG	4 cut(s)	300, 904, 1213, 2279
PleI	GAGTC	2 cut(s)	764, 1905
PluTI	GGCGCC	1 cut(s)	438
PpsI	GAGTC	2 cut(s)	764, 1905
Ppu21I	YACGTR	2 cut(s)	955, 961
PpuMI	RGGWCCY	2 cut(s)	886, 1577
PscI	ACATGT	1 cut(s)	1515
Psp5II	RGGWCCY	2 cut(s)	886, 1577
Psp6I	CCWGG	6 cut(s)	430, 613, 724, 1009, 1251, 1944
PspEI	GGTNACC	1 cut(s)	1849
PspGI	CCWGG	6 cut(s)	430, 613, 724, 1009, 1251, 1944
PspPI	GGNCC	8 cut(s)	4, 143, 291, 375, 799, 886, 1117, 1577
PspPPI	RGGWCCY	2 cut(s)	886, 1577
PspXI	VCTCGAGB	1 cut(s)	326
PsuI	RGATCY	5 cut(s)	308, 529, 993, 1075, 1705
PsyI	GACNNNGTC	3 cut(s)	554, 872, 1187
PvuII	CAGCTG	1 cut(s)	244
RsaI	GTAC	7 cut(s)	959, 983, 1432, 1670, 1937, 2284, 2309
RsaNI	GTAC	7 cut(s)	958, 982, 1431, 1669, 1936, 2283, 2308
RseI	CAYNNNNRTG	2 cut(s)	542, 1536
SalI	GTCGAC	3 cut(s)	41, 623, 1196
SaqAI	TTAA	5 cut(s)	14, 36, 1035, 1134, 1779
Sau3AI	GATC	9 cut(s)	308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
Sau96I	GGNCC	8 cut(s)	4, 143, 291, 375, 799, 886, 1117, 1577
ScaI	AGTACT	1 cut(s)	1670
SchI	GAGTC	2 cut(s)	764, 1906
ScrFI	CCNGG	9 cut(s)	180, 432, 615, 726, 1011, 1053, 1253, 1573, 1946
SduI	GDGCHC	9 cut(s)	234, 644, 672, 722, 1155, 1335, 1406, 2004, 2168
SfaNI	GCATC	4 cut(s)	276, 627, 640, 1261
SfoI	GGCGCC	1 cut(s)	436
Sfr274I	CTCGAG	1 cut(s)	326
SfuI	TTCGAA	1 cut(s)	198
SgrAI	CRCCGGYG	2 cut(s)	812, 2099
SinI	GGWCC	4 cut(s)	291, 886, 1117, 1577
SlaI	CTCGAG	1 cut(s)	326
SmiMI	CAYNNNNRTG	2 cut(s)	542, 1536
SmlI	CTYRAG	2 cut(s)	326, 839
SmoI	CTYRAG	2 cut(s)	326, 839
Sse9I	AATT	7 cut(s)	10, 1737, 1835, 1902, 2019, 2063, 2151
SspDI	GGCGCC	1 cut(s)	434
SspI	AATATT	1 cut(s)	2215
SspMI	CTAG	7 cut(s)	1323, 1560, 1634, 1812, 1958, 2049, 2317
StyD4I	CCNGG	9 cut(s)	178, 430, 613, 724, 1009, 1051, 1251, 1571, 1944
StyI	CCWWGG	7 cut(s)	567, 882, 898, 1354, 1786, 1857, 2273
TaaI	ACNGT	8 cut(s)	667, 1047, 1337, 1430, 1600, 1889, 1926, 2129
TaiI	ACGT	8 cut(s)	42, 108, 123, 384, 456, 957, 963, 1203
TaqI	TCGA	9 cut(s)	42, 198, 327, 354, 450, 624, 1182, 1197, 1302
TasI	AATT	7 cut(s)	10, 1737, 1835, 1902, 2019, 2063, 2151
TatI	WGTACW	3 cut(s)	1668, 1935, 2307
TauI	GCSGC	7 cut(s)	206, 222, 411, 681, 812, 1544, 1725
TfiI	GAWTC	1 cut(s)	495
Tru1I	TTAA	5 cut(s)	14, 36, 1035, 1134, 1779
Tru9I	TTAA	5 cut(s)	14, 36, 1035, 1134, 1779
TseFI	GTSAC	7 cut(s)	320, 1047, 1388, 1451, 1592, 1849, 2035
TseI	GCWGC	3 cut(s)	835, 1553, 1662
Tsp45I	GTSAC	7 cut(s)	320, 1047, 1388, 1451, 1592, 1849, 2035
TspDTI	ATGAA	9 cut(s)	250, 501, 853, 1161, 1336, 1451, 1486, 2255, 2259
TspGWI	ACGGA	2 cut(s)	986, 1162
Tth111I	GACNNNGTC	3 cut(s)	554, 872, 1187
Van91I	CCANNNNNTGG	4 cut(s)	300, 904, 1213, 2279
VneI	GTGCAC	3 cut(s)	640, 668, 1402
VpaK11BI	GGWCC	4 cut(s)	291, 886, 1117, 1577
XagI	CCTNNNNNAGG	3 cut(s)	329, 1019, 1256
XapI	RAATTY	3 cut(s)	10, 1737, 1835
XceI	RCATGY	2 cut(s)	1519, 2092
XcmI	CCANNNNNNNNNTGG	1 cut(s)	793
XhoI	CTCGAG	1 cut(s)	326
XmiI	GTMKAC	3 cut(s)	42, 624, 1197
XmnI	GAANNNNTTC	1 cut(s)	1298
XspI	CTAG	7 cut(s)	1323, 1560, 1634, 1812, 1958, 2049, 2317
ZraI	GACGTC	2 cut(s)	121, 454
ZrmI	AGTACT	1 cut(s)	1670

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.