MD06G1169800.v1.1

Galactinol--sucrose galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005392
Physical Location & Seq
Reverse (-)
31037687 .. 31041456
3770 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1169800.v1.1.491

Sequence Viewer

Length: 2337 bp
ATGGCTCCAAGCCTAACAAAGAATGCCCTAGACGTAATGGGCCTTGCAGTGGATGACACATCATCACAACTCCCAATAACTCTCCATGGATCCAACCTTCTCGCTAATGGCCACCCAATCCTCACTGAAGTCCCTCTCAGCGTCACAGCAACCCTGTCACCTTTTAACAAGACCAAGCCCTCTGGCTGCTTCCTGGGATTCGACGCGGACGAGCCCAAGAGCCGCCACGTGGTACACATCGGAAAACTCATCGGCATCAGGTTCATGAGCATATTCCGGTTCAAGGTCTGGTGGTCCACCCACTGGACAGGAACCACCGGAAACGACGTGGAGCACGAGACCCAGATGATGCTCTTGGACAAAAACGACTCCGGGCGACCCTACGTTGTGATTCTTCCACTCCTGGAAGGACCCTTCAGAGCCTCTCTCCAACCGAGTCATGCAAACGACGACTATGTGGACATGTGCGTGGAAAGCGGCTCGAAACGTGTCAGCGGCTCGAGCTTTAGAAGCTGCTTGTACATGCACGTCGGCGATGACCCTTATAGTTTGGTCCACGAGTCAATGAAGGTGGTGAGGGCCCACTTGGGGACGTTCAAGCTCCTAGAGGAGAAAGATGTACCAAAAATAGTTGACAAGCTTGGGTGGTGTACATGGGATGCGTTCTATCTTATGGTGCACCCCAGAGGGGTGTGGGAAGGTGTGAAGGGCCTAGTGGAAGGTGGGTGCCCTCCAGGACTAGTCCTCATCGACGACGGATGGCAATCGGTCTGCCACGACGAGGACCCGGTGGAGGGGCAAGAAAGCATGACCAGGATATCAGCCGGGGAACAAATGCCAGGCAGGCTGATTAGTTTTCATGAGAATTATAAGTTTAAGGACTATGAGGGTGCGTCTAGCAAGGGCATGGGTGGCTTTATAAAGGATTTGAAGGAGGAGTTTGGGAGTGTGGAGCATGTGTATGTGTGGCATGCGCTTTGTGGATATTGGGGTGGGATTAGGCCAAGGACTAATAGTCCAGGGATGCCTGAAGAGTGCAGGGTGATTAATCCTAAGTTGTCTCCGGGATTGGAGAGGGCAATGGAGGACATGGCGGTGGACAAAATTGTGAGGAATGGAGTAGGGTTGGTTCTGCCGGAGGTGGCGCACAAGTTGTATGAGGGGATGCACTCTTATCTTCAGTCGGCGGGGATCGATGGTGTTAAGGTTGATGTCATACACTTGCTTGAGATGTTATGCGAGGATTTTGGGGGTCGGGTTGAGCTGGCGAAAGCCTACTACAAGGCACTGACTGCTTCCATGAGGAAGCATTTCAATGGCAATGGGGTGATTGCTAGCATGCAGCACTGCAATGACTTTATGTACCTCGGAACCGAGGCCATAGCCCTTGGACGTGTTGGAGATGATTTTTGGTCTGAGACGACAGCGGAAGCAGATGGAACATATTGGCTGCAAGGGTGTCACATGGTGCACTGCGCCTACAATAGCCTTTGGATGGGCAACATCATACAGCCAGATTGGGACATGTTTCAGTCTACTCACCCTTGTGCTGAATTCCATGCTGCATCAAGAGCTATATCCGGAGGACCAATCTACATTAGTGACTCTGTCGGAAAGCACAACTTCAAGCTTCTTAAGAGCATGGTGCTGCCTGATGGGTCCGTACTGCGATGTCAACACTATGCCCTTCCTGCTGGGGATTGCCTATTTGAAGACCCTTTGCATGATGGCAAAACAATGCTCAAAATATGGAACCTCAACAAATGTACTGGAGTTTTGGGTGCTTTTAATTGTCAAGGCGGAGGATGGTGCTCGAAATCCAGGCGGAACAAAATTTTCCCCGACTGCTCGAAGTCTGTGACTTGTTTTGCTAGTCCAAAAGACGTGGAATGGAATCAAGGGAAGAACCCCATTTCAGTACAAGGAGTGACCATATTTGCTGTGTACACGTTCCAGCAGAACAAGCTGAAGCTCTTGAAGCCATCAGAGAAGGTAGAGATTCCACTTGAGCCTTTTAGTTTTGAGCTCCTCACTGTTTCTCCGGTGAGGGTCTTGCGGAAGAAACTGATCCAATTTGCTCCGATTGGATTGGTCAACATGCTCAACACTGGCGGTGCAATTGATTCAGTAGAAGTTGAGGAGCAGGAGGGGAACCCTAGTTTGGTGAGAATTGGAGTGATGGGTTGTGGAGAAATGAGAATGTTTGCTTCAGAAAGGCCAACTGCTTGTGAGATCGATGGAGATGGAGTGAAGTTTGATTATTTTGATAAGATGGTCAGAGTCCAAGTGCCATGGCTTACTTCTTCAAGATCAGTTCTAGTTGACTACTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

779

Amino Acids

86.34

Weight (kDa)

5.89

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 30 - 760 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 870, 920
AccB1I GGYRCC 1 cut(s) 726
AccB7I CCANNNNNTGG 1 cut(s) 303
AccI GTMKAC 1 cut(s) 1535
AccII CGCG 1 cut(s) 206
AccIII TCCGGA 1 cut(s) 1580
AclWI GGATC 4 cut(s) 84, 97, 1199, 2062
AcoI YGGCCR 1 cut(s) 109
AcsI RAATTY 2 cut(s) 1553, 1833
AcuI CTGAAG 6 cut(s) 147, 400, 1050, 1163, 1988, 2193
AcvI CACGTG 1 cut(s) 229
AdeI CACNNNGTG 1 cut(s) 1468
AfaI GTAC 9 cut(s) 234, 521, 621, 652, 1364, 1665, 1768, 1920, 1946
AflII CTTAAG 1 cut(s) 1634
AflIII ACRYGT 5 cut(s) 462, 487, 1393, 1524, 1947
AgsI TTSAA 8 cut(s) 283, 598, 931, 1315, 1627, 1712, 1978, 2307
AhdI GACNNNNNGTC 1 cut(s) 1014
AhlI ACTAGT 1 cut(s) 739
AjiI CACGTC 4 cut(s) 328, 529, 1394, 1885
AjnI CCWGG 7 cut(s) 192, 402, 733, 812, 838, 1018, 1820
AjuI GAANNNNNNNTTGG 2 cut(s) 2144, 2176
AleI CACNNNNGTG 1 cut(s) 1545
Alw21I GWGCWC 5 cut(s) 336, 681, 1473, 1814, 2028
Alw26I GTCTC 3 cut(s) 332, 1065, 1412
Alw44I GTGCAC 2 cut(s) 677, 1469
AlwI GGATC 4 cut(s) 84, 97, 1199, 2062
Ama87I CYCGRG 1 cut(s) 499
Aor13HI TCCGGA 1 cut(s) 1580
AoxI GGCC 7 cut(s) 40, 109, 579, 709, 1001, 1377, 2216
ApaI GGGCCC 1 cut(s) 583
ApaLI GTGCAC 2 cut(s) 677, 1469
ApeKI GCWGC 6 cut(s) 186, 513, 1342, 1450, 1562, 1648
ApoI RAATTY 2 cut(s) 1553, 1833
AseI ATTAAT 1 cut(s) 1047
Asp700I GAANNNNTTC 1 cut(s) 1310
AspLEI GCGC 3 cut(s) 976, 1147, 1478
AsuC2I CCSGG 4 cut(s) 373, 788, 826, 1065
AsuHPI GGTGA 7 cut(s) 150, 586, 1054, 1339, 1532, 2056, 2176
AsuNHI GCTAGC 1 cut(s) 1334
AvaI CYCGRG 1 cut(s) 499
AvaII GGWCC 6 cut(s) 294, 410, 553, 784, 1586, 1659
BaeGI GKGCMC 4 cut(s) 583, 681, 731, 1473
BalI TGGCCA 1 cut(s) 111
BamHI GGATCC 1 cut(s) 89
BanI GGYRCC 1 cut(s) 726
BanII GRGCYC 3 cut(s) 216, 583, 2028
BauI CACGAG 2 cut(s) 335, 557
BbrPI CACGTG 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 1719
Bbv12I GWGCWC 5 cut(s) 336, 681, 1473, 1814, 2028
BbvI GCAGC 6 cut(s) 173, 500, 1354, 1437, 1549, 1635
BciT130I CCWGG 7 cut(s) 194, 404, 735, 814, 840, 1020, 1822
BcnI CCSGG 4 cut(s) 373, 788, 826, 1065
BcoDI GTCTC 3 cut(s) 332, 1065, 1412
BcuI ACTAGT 1 cut(s) 739
BfaI CTAG 9 cut(s) 29, 605, 713, 740, 897, 1335, 1872, 2157, 2318
BfrI CTTAAG 1 cut(s) 1634
BglI GCCNNNNNGGC 1 cut(s) 844
BisI GCNGC 9 cut(s) 187, 223, 478, 496, 514, 1343, 1451, 1563, 1649
BlsI GCNGC 9 cut(s) 188, 224, 479, 497, 515, 1344, 1452, 1564, 1650
Bme18I GGWCC 6 cut(s) 294, 410, 553, 784, 1586, 1659
BmeRI GACNNNNNGTC 1 cut(s) 1014
BmeT110I CYCGRG 1 cut(s) 499
BmgBI CACGTC 4 cut(s) 328, 529, 1394, 1885
BmsI GCATC 6 cut(s) 264, 339, 649, 1014, 1155, 1574
BmtI GCTAGC 1 cut(s) 1338
BpiI GAAGAC 1 cut(s) 1719
BplI GAGNNNNNCTC 2 cut(s) 411, 443
BpmI CTGGAG 2 cut(s) 717, 1791
BpuEI CTTGAG 2 cut(s) 1247, 2027
BpuMI CCSGG 4 cut(s) 373, 788, 826, 1065
Bsa29I ATCGAT 2 cut(s) 1194, 2235
BsaAI YACGTR 1 cut(s) 229
BsaBI GATNNNNATC 1 cut(s) 763
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 9 cut(s) 85, 193, 825, 1004, 1019, 1366, 1374, 1387, 2291
BsaWI WCCGGW 4 cut(s) 276, 317, 1580, 2041
BsaXI ACNNNNNCTCC 6 cut(s) 944, 974, 2023, 2053, 2232, 2262
Bse1I ACTGG 3 cut(s) 308, 1774, 2113
Bse3DI GCAATG 3 cut(s) 1086, 1327, 1357
Bse8I GATNNNNATC 1 cut(s) 763
BseAI TCCGGA 1 cut(s) 1580
BseBI CCWGG 7 cut(s) 194, 404, 735, 814, 840, 1020, 1822
BseCI ATCGAT 2 cut(s) 1194, 2235
BseDI CCNNGG 9 cut(s) 85, 193, 825, 1004, 1019, 1366, 1374, 1387, 2291
BseGI GGATG 7 cut(s) 58, 664, 764, 1029, 1170, 1500, 1811
BseJI GATNNNNATC 1 cut(s) 763
BseMI GCAATG 3 cut(s) 1086, 1327, 1357
BseMII CTCAG 2 cut(s) 151, 1407
BseNI ACTGG 3 cut(s) 308, 1774, 2113
BseRI GAGGAG 4 cut(s) 623, 950, 2018, 2153
BseSI GKGCMC 4 cut(s) 583, 681, 731, 1473
BseXI GCAGC 6 cut(s) 173, 500, 1354, 1437, 1549, 1635
BseYI CCCAGC 1 cut(s) 1694
BsgI GTGCAG 1 cut(s) 1057
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 7 cut(s) 42, 111, 581, 711, 1003, 1379, 2218
BshNI GGYRCC 1 cut(s) 726
BshVI ATCGAT 2 cut(s) 1194, 2235
BsiHKAI GWGCWC 5 cut(s) 336, 681, 1473, 1814, 2028
BsiHKCI CYCGRG 1 cut(s) 499
BsiSI CCGG 9 cut(s) 277, 318, 372, 788, 825, 1064, 1136, 1581, 2042
BslFI GGGAC 3 cut(s) 116, 604, 1535
BsmAI GTCTC 3 cut(s) 332, 1065, 1412
BsmBI CGTCTC 1 cut(s) 1412
BsmFI GGGAC 3 cut(s) 116, 604, 1535
BsmI GAATGC 1 cut(s) 28
BsnI GGCC 7 cut(s) 42, 111, 581, 711, 1003, 1379, 2218
Bso31I GGTCTC 1 cut(s) 332
BsoBI CYCGRG 1 cut(s) 499
Bsp120I GGGCCC 1 cut(s) 579
Bsp1286I GDGCHC 8 cut(s) 216, 336, 583, 681, 731, 1473, 1814, 2028
Bsp13I TCCGGA 1 cut(s) 1580
Bsp1407I TGTACA 3 cut(s) 519, 650, 1944
Bsp143I GATC 5 cut(s) 89, 1191, 2067, 2232, 2309
Bsp19I CCATGG 2 cut(s) 85, 2291
BspANI GGCC 7 cut(s) 42, 111, 581, 711, 1003, 1379, 2218
BspCNI CTCAG 2 cut(s) 150, 1408
BspDI ATCGAT 2 cut(s) 1194, 2235
BspEI TCCGGA 1 cut(s) 1580
BspFNI CGCG 1 cut(s) 206
BspHI TCATGA 2 cut(s) 264, 859
BspOI GCTAGC 1 cut(s) 1338
BspPI GGATC 4 cut(s) 84, 97, 1199, 2062
BspT107I GGYRCC 1 cut(s) 726
BspTI CTTAAG 1 cut(s) 1634
BspTNI GGTCTC 1 cut(s) 332
BsrDI GCAATG 3 cut(s) 1086, 1327, 1357
BsrGI TGTACA 3 cut(s) 519, 650, 1944
BsrI ACTGG 3 cut(s) 308, 1774, 2113
BssECI CCNNGG 9 cut(s) 85, 193, 825, 1004, 1019, 1366, 1374, 1387, 2291
BssMI GATC 5 cut(s) 89, 1191, 2067, 2232, 2309
BssSI CACGAG 2 cut(s) 335, 557
BssT1I CCWWGG 4 cut(s) 85, 1004, 1387, 2291
Bst2BI CACGAG 2 cut(s) 335, 557
Bst2UI CCWGG 7 cut(s) 194, 404, 735, 814, 840, 1020, 1822
Bst4CI ACNGT 1 cut(s) 2035
Bst6I CTCTTC 1 cut(s) 1026
BstAFI CTTAAG 1 cut(s) 1634
BstAPI GCANNNNNTGC 1 cut(s) 1292
BstAUI TGTACA 3 cut(s) 519, 650, 1944
BstBAI YACGTR 1 cut(s) 229
BstC8I GCNNGC 5 cut(s) 845, 972, 1266, 1336, 1340
BstDEI CTNAG 3 cut(s) 137, 1053, 1416
BstDSI CCRYGG 2 cut(s) 85, 2291
BstF5I GGATG 7 cut(s) 58, 664, 764, 1029, 1170, 1500, 1811
BstFNI CGCG 1 cut(s) 206
BstHHI GCGC 3 cut(s) 976, 1147, 1478
BstKTI GATC 5 cut(s) 92, 1194, 2070, 2235, 2312
BstMAI GTCTC 3 cut(s) 332, 1065, 1412
BstMBI GATC 5 cut(s) 89, 1191, 2067, 2232, 2309
BstNI CCWGG 7 cut(s) 194, 404, 735, 814, 840, 1020, 1822
BstNSI RCATGY 7 cut(s) 466, 526, 959, 974, 1342, 1528, 2101
BstSLI GKGCMC 4 cut(s) 583, 681, 731, 1473
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 6 cut(s) 173, 500, 1354, 1437, 1549, 1635
BstV2I GAAGAC 1 cut(s) 1719
BstX2I RGATCY 1 cut(s) 89
BstYI RGATCY 1 cut(s) 89
Bsu15I ATCGAT 2 cut(s) 1194, 2235
BsuRI GGCC 7 cut(s) 42, 111, 581, 711, 1003, 1379, 2218
BsuTUI ATCGAT 2 cut(s) 1194, 2235
BtgI CCRYGG 2 cut(s) 85, 2291
BtgZI GCGATG 2 cut(s) 549, 1684
BtrI CACGTC 4 cut(s) 328, 529, 1394, 1885
BtsCI GGATG 7 cut(s) 58, 664, 764, 1029, 1170, 1500, 1811
BtsI GCAGTG 3 cut(s) 54, 1345, 1471
BtsIMutI CAGTG 8 cut(s) 54, 123, 301, 1286, 1345, 1471, 2031, 2106
Cac8I GCNNGC 5 cut(s) 845, 972, 1266, 1336, 1340
CciI TCATGA 2 cut(s) 264, 859
CfoI GCGC 3 cut(s) 976, 1147, 1478
ClaI ATCGAT 2 cut(s) 1194, 2235
CseI GACGC 3 cut(s) 130, 212, 882
Csp6I GTAC 9 cut(s) 233, 520, 620, 651, 1363, 1664, 1767, 1919, 1945
CspCI CAANNNNNGTGG 4 cut(s) 552, 587, 1866, 1901
CviQI GTAC 9 cut(s) 233, 520, 620, 651, 1363, 1664, 1767, 1919, 1945
DdeI CTNAG 3 cut(s) 137, 1053, 1416
DpnI GATC 5 cut(s) 91, 1193, 2069, 2234, 2311
DpnII GATC 5 cut(s) 89, 1191, 2067, 2232, 2309
DraIII CACNNNGTG 1 cut(s) 1468
DriI GACNNNNNGTC 1 cut(s) 1014
EaeI YGGCCR 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 1026
Eam1105I GACNNNNNGTC 1 cut(s) 1014
EarI CTCTTC 1 cut(s) 1026
EciI GGCGGA 2 cut(s) 1815, 1840
Ecl136II GAGCTC 1 cut(s) 2026
Eco130I CCWWGG 4 cut(s) 85, 1004, 1387, 2291
Eco24I GRGCYC 3 cut(s) 216, 583, 2028
Eco31I GGTCTC 1 cut(s) 332
Eco32I GATATC 1 cut(s) 819
Eco47I GGWCC 6 cut(s) 294, 410, 553, 784, 1586, 1659
Eco53kI GAGCTC 1 cut(s) 2026
Eco57I CTGAAG 6 cut(s) 147, 400, 1050, 1163, 1988, 2193
Eco72I CACGTG 1 cut(s) 229
Eco88I CYCGRG 1 cut(s) 499
EcoICRI GAGCTC 1 cut(s) 2026
EcoO109I RGGNCCY 4 cut(s) 410, 579, 709, 784
EcoRI GAATTC 1 cut(s) 1553
EcoRII CCWGG 7 cut(s) 192, 402, 733, 812, 838, 1018, 1820
EcoRV GATATC 1 cut(s) 819
EcoT14I CCWWGG 4 cut(s) 85, 1004, 1387, 2291
EcoT38I GRGCYC 3 cut(s) 216, 583, 2028
ErhI CCWWGG 4 cut(s) 85, 1004, 1387, 2291
Esp3I CGTCTC 1 cut(s) 1412
FalI AAGNNNNNCTT 2 cut(s) 1607, 1639
FaqI GGGAC 3 cut(s) 116, 604, 1535
FauI CCCGC 1 cut(s) 1180
FblI GTMKAC 1 cut(s) 1535
Fnu4HI GCNGC 9 cut(s) 187, 223, 478, 496, 514, 1343, 1451, 1563, 1649
FokI GGATG 7 cut(s) 65, 671, 771, 1036, 1177, 1507, 1818
FriOI GRGCYC 3 cut(s) 216, 583, 2028
Fsp4HI GCNGC 9 cut(s) 187, 223, 478, 496, 514, 1343, 1451, 1563, 1649
FspBI CTAG 9 cut(s) 29, 605, 713, 740, 897, 1335, 1872, 2157, 2318
GlaI GCGC 3 cut(s) 975, 1146, 1477
GluI GCNGC 9 cut(s) 187, 223, 478, 496, 514, 1343, 1451, 1563, 1649
GsaI CCCAGC 1 cut(s) 1698
GsuI CTGGAG 2 cut(s) 717, 1791
HaeIII GGCC 7 cut(s) 42, 111, 581, 711, 1003, 1379, 2218
HapII CCGG 9 cut(s) 277, 318, 372, 788, 825, 1064, 1136, 1581, 2042
HgaI GACGC 3 cut(s) 130, 212, 882
HhaI GCGC 3 cut(s) 976, 1147, 1478
Hin6I GCGC 3 cut(s) 974, 1145, 1476
HinP1I GCGC 3 cut(s) 974, 1145, 1476
HincII GTYRAC 4 cut(s) 634, 1676, 2095, 2323
HindII GTYRAC 4 cut(s) 634, 1676, 2095, 2323
HindIII AAGCTT 2 cut(s) 638, 1628
HpaII CCGG 9 cut(s) 277, 318, 372, 788, 825, 1064, 1136, 1581, 2042
HphI GGTGA 7 cut(s) 150, 586, 1054, 1339, 1532, 2056, 2176
Hpy188I TCNGA 9 cut(s) 242, 419, 1370, 1417, 1613, 1987, 2082, 2212, 2279
Hpy188III TCNNGA 6 cut(s) 265, 860, 1569, 1581, 1975, 2307
Hpy99I CGWCG 7 cut(s) 206, 329, 452, 533, 755, 758, 782
HpyCH4III ACNGT 1 cut(s) 2035
HpyF3I CTNAG 3 cut(s) 137, 1053, 1416
HspAI GCGC 3 cut(s) 974, 1145, 1476
Kpn2I TCCGGA 1 cut(s) 1580
Kzo9I GATC 5 cut(s) 89, 1191, 2067, 2232, 2309
LmnI GCTCC 7 cut(s) 10, 331, 606, 952, 2031, 2083, 2140
Lsp1109I GCAGC 6 cut(s) 173, 500, 1354, 1437, 1549, 1635
LweI GCATC 6 cut(s) 264, 339, 649, 1014, 1155, 1574
MaeI CTAG 9 cut(s) 29, 605, 713, 740, 897, 1335, 1872, 2157, 2318
MaeIII GTNAC 6 cut(s) 142, 156, 1460, 1601, 1858, 1927
MalI GATC 5 cut(s) 91, 1193, 2069, 2234, 2311
MboI GATC 5 cut(s) 89, 1191, 2067, 2232, 2309
MboII GAAGA 7 cut(s) 386, 1043, 1169, 1724, 1915, 2071, 2295
MfeI CAATTG 1 cut(s) 2118
MflI RGATCY 1 cut(s) 89
MhlI GDGCHC 8 cut(s) 216, 336, 583, 681, 731, 1473, 1814, 2028
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 8 cut(s) 865, 1104, 1553, 1789, 1833, 2072, 2118, 2169
MluNI TGGCCA 1 cut(s) 111
MlyI GAGTC 5 cut(s) 362, 445, 569, 1598, 2289
MmeI TCCRAC 4 cut(s) 117, 454, 1378, 1591
Mox20I TGGCCA 1 cut(s) 111
MroI TCCGGA 1 cut(s) 1580
MroXI GAANNNNTTC 1 cut(s) 1310
MscI TGGCCA 1 cut(s) 111
MseI TTAA 6 cut(s) 165, 876, 1047, 1203, 1635, 1788
MslI CAYNNNNRTG 6 cut(s) 467, 960, 1094, 1314, 1350, 1545
Msp20I TGGCCA 1 cut(s) 111
MspA1I CMGCKG 2 cut(s) 495, 1427
MspCI CTTAAG 1 cut(s) 1634
MspI CCGG 9 cut(s) 277, 318, 372, 788, 825, 1064, 1136, 1581, 2042
MunI CAATTG 1 cut(s) 2118
Mva1269I GAATGC 1 cut(s) 28
MvaI CCWGG 7 cut(s) 194, 404, 735, 814, 840, 1020, 1822
MvnI CGCG 1 cut(s) 206
NciI CCSGG 4 cut(s) 373, 788, 826, 1065
NcoI CCATGG 2 cut(s) 85, 2291
NdeII GATC 5 cut(s) 89, 1191, 2067, 2232, 2309
NheI GCTAGC 1 cut(s) 1334
NmuCI GTSAC 6 cut(s) 142, 156, 1460, 1601, 1858, 1927
NspI RCATGY 7 cut(s) 466, 526, 959, 974, 1342, 1528, 2101
OliI CACNNNNGTG 1 cut(s) 1545
PaeI GCATGC 2 cut(s) 974, 1342
PaeR7I CTCGAG 1 cut(s) 499
PagI TCATGA 2 cut(s) 264, 859
PciI ACATGT 2 cut(s) 462, 1524
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 333
PctI GAATGC 1 cut(s) 28
PdmI GAANNNNTTC 1 cut(s) 1310
PfeI GAWTC 5 cut(s) 198, 391, 1894, 1999, 2123
PflFI GACNNNGTC 1 cut(s) 1607
PflMI CCANNNNNTGG 1 cut(s) 303
PfoI TCCNGGA 3 cut(s) 402, 733, 1063
PkrI GCNGC 9 cut(s) 188, 224, 479, 497, 515, 1344, 1452, 1564, 1650
PleI GAGTC 5 cut(s) 362, 444, 568, 1598, 2288
PmaCI CACGTG 1 cut(s) 229
PmlI CACGTG 1 cut(s) 229
PpsI GAGTC 5 cut(s) 362, 444, 568, 1598, 2288
Ppu21I YACGTR 1 cut(s) 229
PpuMI RGGWCCY 2 cut(s) 410, 784
PscI ACATGT 2 cut(s) 462, 1524
PshBI ATTAAT 1 cut(s) 1047
PsiI TTATAA 2 cut(s) 870, 920
Psp124BI GAGCTC 1 cut(s) 2028
Psp5II RGGWCCY 2 cut(s) 410, 784
Psp6I CCWGG 7 cut(s) 192, 402, 733, 812, 838, 1018, 1820
PspCI CACGTG 1 cut(s) 229
PspFI CCCAGC 1 cut(s) 1694
PspGI CCWGG 7 cut(s) 192, 402, 733, 812, 838, 1018, 1820
PspOMI GGGCCC 1 cut(s) 579
PspPPI RGGWCCY 2 cut(s) 410, 784
PspXI VCTCGAGB 1 cut(s) 499
PsrI GAACNNNNNNTAC 2 cut(s) 1113, 1145
PsuI RGATCY 1 cut(s) 89
PsyI GACNNNGTC 1 cut(s) 1607
RsaI GTAC 9 cut(s) 234, 521, 621, 652, 1364, 1665, 1768, 1920, 1946
RsaNI GTAC 9 cut(s) 233, 520, 620, 651, 1363, 1664, 1767, 1919, 1945
RseI CAYNNNNRTG 6 cut(s) 467, 960, 1094, 1314, 1350, 1545
SacI GAGCTC 1 cut(s) 2028
SaqAI TTAA 6 cut(s) 165, 876, 1047, 1203, 1635, 1788
SatI GCNGC 9 cut(s) 187, 223, 478, 496, 514, 1343, 1451, 1563, 1649
Sau3AI GATC 5 cut(s) 89, 1191, 2067, 2232, 2309
SchI GAGTC 5 cut(s) 362, 445, 569, 1598, 2289
SduI GDGCHC 8 cut(s) 216, 336, 583, 681, 731, 1473, 1814, 2028
SfaNI GCATC 6 cut(s) 264, 339, 649, 1014, 1155, 1574
Sfr274I CTCGAG 1 cut(s) 499
SinI GGWCC 6 cut(s) 294, 410, 553, 784, 1586, 1659
SlaI CTCGAG 1 cut(s) 499
SmiMI CAYNNNNRTG 6 cut(s) 467, 960, 1094, 1314, 1350, 1545
SmlI CTYRAG 4 cut(s) 499, 1226, 1634, 2006
SmoI CTYRAG 4 cut(s) 499, 1226, 1634, 2006
SpeI ACTAGT 1 cut(s) 739
SphI GCATGC 2 cut(s) 974, 1342
Sse9I AATT 8 cut(s) 865, 1104, 1553, 1789, 1833, 2072, 2118, 2169
SspMI CTAG 9 cut(s) 29, 605, 713, 740, 897, 1335, 1872, 2157, 2318
SstI GAGCTC 1 cut(s) 2028
StyI CCWWGG 4 cut(s) 85, 1004, 1387, 2291
TaaI ACNGT 1 cut(s) 2035
TaqI TCGA 8 cut(s) 201, 482, 500, 750, 1194, 1814, 1850, 2235
TaqII GACCGA 1 cut(s) 757
TasI AATT 8 cut(s) 865, 1104, 1553, 1789, 1833, 2072, 2118, 2169
TatI WGTACW 5 cut(s) 519, 650, 1766, 1918, 1944
TauI GCSGC 3 cut(s) 225, 480, 498
TfiI GAWTC 5 cut(s) 198, 391, 1894, 1999, 2123
Tru1I TTAA 6 cut(s) 165, 876, 1047, 1203, 1635, 1788
Tru9I TTAA 6 cut(s) 165, 876, 1047, 1203, 1635, 1788
TscAI CASTG 8 cut(s) 54, 130, 308, 1293, 1352, 1478, 2038, 2113
TseFI GTSAC 6 cut(s) 142, 156, 1460, 1601, 1858, 1927
TseI GCWGC 6 cut(s) 186, 513, 1342, 1450, 1562, 1648
Tsp45I GTSAC 6 cut(s) 142, 156, 1460, 1601, 1858, 1927
TspDTI ATGAA 3 cut(s) 253, 581, 848
TspGWI ACGGA 2 cut(s) 771, 1651
TspRI CASTG 8 cut(s) 54, 130, 308, 1293, 1352, 1478, 2038, 2113
Tth111I GACNNNGTC 1 cut(s) 1607
Van91I CCANNNNNTGG 1 cut(s) 303
Vha464I CTTAAG 1 cut(s) 1634
VneI GTGCAC 2 cut(s) 677, 1469
VpaK11BI GGWCC 6 cut(s) 294, 410, 553, 784, 1586, 1659
VspI ATTAAT 1 cut(s) 1047
XapI RAATTY 2 cut(s) 1553, 1833
XceI RCATGY 7 cut(s) 466, 526, 959, 974, 1342, 1528, 2101
XhoI CTCGAG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 1535
XmnI GAANNNNTTC 1 cut(s) 1310
XspI CTAG 9 cut(s) 29, 605, 713, 740, 897, 1335, 1872, 2157, 2318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.