Rroxscaffold_3G00262500

Galactinol--sucrose galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005392
Physical Location & Seq
Forward (+)
56516616 .. 56521934
5319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262500.1

Sequence Viewer

Length: 2319 bp
ATGGCTCCAAGCCTAAGCAAGAACAATGCCGCAGACGAAATGGGCCTTGTAGACAGCAACTCACCACCGTCAATAACACTACAAGGCACAAACTTCCTCGCCAACGGCCACCCAATCCTCACTGAAGTCCCACTAAACATCACAGCCACTCCATCACCAAACAAAACCACCGTGGGCTGCTTCATTGGCTTCGACGCCGACGAGCCCAAAAGCCGCCATGTGGAGCCGCTCGGCAAGCTCAGCGGCATCAAGTTCATGAGCATTTTCAGGTTCAAAGTCTGGTGGACCACCCAGTGGGTCGGAACAACCGGAAACGACGTGCAGCACGAGACTCAGTTCATGATCCTCGACAAAAAAGACTCCACCGGCCGCCCCTACGTCCTCCTCCTTCCACTTCTCGAAGGTCCCTTCAGAGCCTCCCTCCAACCTGGCTCCGACTGCCAAGTGGACATGTGCGTCGAGAGTGGCTCGACACGTGTCAGTGAATCCACCTTCCAGAGCGTTCTCTACATGCACGTCGGCAACGACCCGTACGGTCTTGTCAAAGAGGCCATGGAGGTGGTGAGAGTCCATTTGGGAAGCTTCAAGCTTCTGGAGGAGAAGACGCCTCCGCCTATAGTGGACAAGTTTGGTTGGTGCACGTGGGATGCGTTTTATCTTATGGTGCACCCCAAGGGAGTTTGGGAAGGGGTTAAGGGCCTAGTGGAGGGTGGGTGTCCTCCGGGAATGGTACTCATCGACGACGGCTGGCAGTCCATTTGTCACGATGACGACCCCCTAACCGACGAAGAAGCCATGAAAAGGACATCTGCCGGTGAGCAAATGCCATGTAGGCTCACAAGTTTCGTAGAGAACTACAAGTTCAGAGACTATAAGAGCCCTAGTGGTAAGGGCATGGGTGCCTTTGTAAAGGACTTGAAGGAGGAGTTTGGGAGCGTGGACTATGTTTATGTTTGGCACGCGCTATGTGGGTATTGGGGTGGGGTTAGACCCGGGGTGAAGAGCGGGTCGGGTTTTCCGGAGAGCAGGGTAATTAGGCCTAAGCTGTCGGAGGGTTTGGAGAAGACGATGGAGGATTTGGCGGTGGACAAGATCGTGAACAATGGAGTGGGGCTGGTGGAACCGGAGATGGCCCACAAGTTGTATGAGGGGCTTCATCAGCATCTTCAGTCGGTCGGGATCGACGGTGTCAAGGTCGATGTCATACACTTGCTTGAGATGTTATCCGAGGAGTTTGGTGGTCGGATTGAGCTGGCCAAAGCTTATTACAAGGCACTTACTGCTTCTGTGAGGAAACATTTCAAAGGCAATGGAGTGATTGCTAGCATGGAGCACTGCAATGATTTCATGTATCTTGGCACAGAAGCCATTTCACTTGGTCGTGTTGGAGACGACTTTTGGTGCTCTGACCCGTCTGGAGATCCAAATGGGACATATTGGCTACAAGGGTGTCACATGGTCCATTGTGCCTACAATAGCTTGTGGATGGGGAACTTCATACATCCAGATTGGGACATGTTTCAGTCGACTCACCCTTGTGCTGCATTTCATGCTGCATCAAGAGCCATATCGGGAGGGCCGATCTATATCAGCGATTTTGTGGGACAGCACAACTTCAAGCTTCTTAAGAGTCTGGTGTTGCCTGATGGATCCATATTAAGATGCCAATACTATGCACTTCCTACTAGGGATTGCCTATTTGAGGACCCTGTTCATGATGGGAAAACAATGCTCAAGATTTGGAACCTCAACAAGTACACCGGCGTTCTAGGACTTTTTAACTGCCAAGGAGGAGGGTGGTGCCCCAAATCAAGGCGCAACAAAAGCTTCCCTGAGTTCTCAAAGTCCGTGATTTGTTTGGCAACCCCAAAAGATGTGGAATGGAGTCATGGGAAGAACCCGATTCCAATAAAAGAAATTGACGCATTTGCTGTGTACACGTTCCAGGAAAAGAAGCTGAAGCTTTTGAAGCCAGTGGAGGCGGTAGAGATTTCACTTCAGCCTTTCAGTTATGAGCTCCTCACCATTGCTCCAGTGAGAGTGCTACCAACAAAGTTGATCCAATTTGCTCCAATTGGATTGGTGAACATGCTCAACACTGGTGGTGCAATTCAGTCACTAGAATTTGAGGATGGTGAAACTGTCGTGAGGATTGGAGTGAAGGGCAGTGGAGAGATGAGAACATTTGCATCAGAAAAACCAACTGCTTGTAAGATTGGTGGAGAGGAAGTGAAATTTGATTATGTTGATAAGATGGTTATTGTCCAAGTGCAATGGCCTAGCTCTTCAATAGCAACTGTGGTTGAGTACTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

772

Amino Acids

85.62

Weight (kDa)

5.75

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 30 - 756 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 455
AccB1I GGYRCC 2 cut(s) 899, 1800
AccB7I CCANNNNNTGG 1 cut(s) 294
AccBSI CCGCTC 2 cut(s) 229, 1005
AccI GTMKAC 2 cut(s) 51, 1526
AccII CGCG 1 cut(s) 962
AccIII TCCGGA 1 cut(s) 1018
AciI CCGC 9 cut(s) 30, 214, 227, 243, 370, 611, 1005, 1082, 1982
AclWI GGATC 6 cut(s) 337, 1187, 1415, 1644, 1657, 2053
AcoI YGGCCR 3 cut(s) 106, 367, 1254
AcsI RAATTY 2 cut(s) 2123, 2234
AcuI CTGAAG 5 cut(s) 144, 394, 1151, 1979, 1982
AcvI CACGTG 2 cut(s) 476, 642
AcyI GRCGYC 2 cut(s) 195, 605
AdeI CACNNNGTG 1 cut(s) 294
AfaI GTAC 5 cut(s) 533, 732, 1757, 1937, 2309
AfiI CCNNNNNNNGG 6 cut(s) 220, 294, 706, 801, 1702, 1812
AflII CTTAAG 1 cut(s) 1625
AflIII ACRYGT 5 cut(s) 450, 473, 475, 1515, 1938
AgsI TTSAA 7 cut(s) 274, 586, 919, 1303, 1618, 1969, 2289
AjiI CACGTC 2 cut(s) 319, 517
AjnI CCWGG 2 cut(s) 427, 1944
AleI CACNNNNGTG 1 cut(s) 1536
Alw21I GWGCWC 5 cut(s) 641, 669, 1335, 1406, 2019
Alw26I GTCTC 3 cut(s) 323, 861, 1383
Alw44I GTGCAC 2 cut(s) 637, 665
AlwI GGATC 6 cut(s) 337, 1187, 1415, 1644, 1657, 2053
Ama87I CYCGRG 1 cut(s) 992
Aor13HI TCCGGA 1 cut(s) 1018
ApaLI GTGCAC 2 cut(s) 637, 665
ApeKI GCWGC 4 cut(s) 177, 322, 1541, 1553
ApoI RAATTY 2 cut(s) 2123, 2234
Asp700I GAANNNNTTC 1 cut(s) 1298
AspLEI GCGC 2 cut(s) 964, 1818
AspS9I GGNCC 8 cut(s) 43, 285, 404, 697, 1132, 1459, 1577, 1705
AsuC2I CCSGG 3 cut(s) 723, 993, 994
AsuHPI GGTGA 9 cut(s) 54, 147, 574, 827, 1009, 1523, 2014, 2095, 2147
AsuNHI GCTAGC 1 cut(s) 1322
AvaI CYCGRG 1 cut(s) 992
AvaII GGWCC 4 cut(s) 285, 404, 1459, 1705
BaeGI GKGCMC 3 cut(s) 641, 669, 1805
BalI TGGCCA 1 cut(s) 1256
BamHI GGATCC 1 cut(s) 1649
BanI GGYRCC 2 cut(s) 899, 1800
BanII GRGCYC 3 cut(s) 207, 881, 2019
BauI CACGAG 1 cut(s) 326
BbrPI CACGTG 2 cut(s) 476, 642
BbsI GAAGAC 2 cut(s) 608, 1070
Bbv12I GWGCWC 5 cut(s) 641, 669, 1335, 1406, 2019
BbvI GCAGC 4 cut(s) 164, 334, 1528, 1540
BccI CCATC 8 cut(s) 160, 1063, 1123, 1480, 1640, 1712, 2126, 2248
BceAI ACGGC 2 cut(s) 121, 760
BciT130I CCWGG 2 cut(s) 429, 1946
BcnI CCSGG 3 cut(s) 723, 993, 994
BcoDI GTCTC 3 cut(s) 323, 861, 1383
BfaI CTAG 7 cut(s) 701, 882, 1323, 1686, 1769, 2120, 2280
BfmI CTRYAG 1 cut(s) 615
BfrI CTTAAG 1 cut(s) 1625
BglI GCCNNNNNGGC 1 cut(s) 832
BisI GCNGC 9 cut(s) 30, 178, 214, 227, 244, 323, 370, 1542, 1554
BlpI GCTNAGC 1 cut(s) 239
BlsI GCNGC 9 cut(s) 31, 179, 215, 228, 245, 324, 371, 1543, 1555
BmcAI AGTACT 1 cut(s) 2309
Bme1390I CCNGG 5 cut(s) 429, 723, 993, 994, 1946
Bme18I GGWCC 4 cut(s) 285, 404, 1459, 1705
BmeT110I CYCGRG 1 cut(s) 992
BmgBI CACGTC 2 cut(s) 319, 517
BmgT120I GGNCC 8 cut(s) 43, 285, 404, 697, 1132, 1459, 1577, 1705
BmrFI CCNGG 5 cut(s) 429, 723, 993, 994, 1946
BmrI ACTGGG 1 cut(s) 286
BmsI GCATC 6 cut(s) 255, 637, 1171, 1565, 1652, 2198
BmtI GCTAGC 1 cut(s) 1326
BmuI ACTGGG 1 cut(s) 286
BoxI GACNNNNGTC 1 cut(s) 476
BpiI GAAGAC 2 cut(s) 608, 1070
BplI GAGNNNNNCTC 4 cut(s) 405, 437, 452, 484
BpmI CTGGAG 3 cut(s) 614, 1437, 2016
Bpu10I CCTNAGC 2 cut(s) 14, 1041
Bpu1102I GCTNAGC 1 cut(s) 239
BpuEI CTTGAG 2 cut(s) 1235, 1718
BpuMI CCSGG 3 cut(s) 723, 993, 994
BsaAI YACGTR 2 cut(s) 476, 642
BsaBI GATNNNNATC 1 cut(s) 1586
BsaHI GRCGYC 2 cut(s) 195, 605
BsaJI CCNNGG 7 cut(s) 171, 552, 672, 992, 993, 1227, 1786
BsaWI WCCGGW 3 cut(s) 308, 1018, 1123
BsaXI ACNNNNNCTCC 8 cut(s) 133, 163, 698, 728, 2014, 2044, 2214, 2244
Bsc4I CCNNNNNNNGG 6 cut(s) 220, 294, 706, 801, 1702, 1812
Bse118I RCCGGY 3 cut(s) 365, 812, 1760
Bse1I ACTGG 4 cut(s) 292, 1973, 2033, 2104
Bse3DI GCAATG 4 cut(s) 1315, 1345, 2025, 2279
Bse8I GATNNNNATC 1 cut(s) 1586
BseAI TCCGGA 1 cut(s) 1018
BseBI CCWGG 2 cut(s) 429, 1946
BseDI CCNNGG 7 cut(s) 171, 552, 672, 992, 993, 1227, 1786
BseGI GGATG 4 cut(s) 652, 1491, 1501, 2137
BseJI GATNNNNATC 1 cut(s) 1586
BseLI CCNNNNNNNGG 6 cut(s) 220, 294, 706, 801, 1702, 1812
BseMI GCAATG 4 cut(s) 1315, 1345, 2025, 2279
BseMII CTCAG 3 cut(s) 253, 347, 1824
BseNI ACTGG 4 cut(s) 292, 1973, 2033, 2104
BseRI GAGGAG 6 cut(s) 374, 611, 938, 1244, 1806, 2009
BseSI GKGCMC 3 cut(s) 641, 669, 1805
BseX3I CGGCCG 1 cut(s) 367
BseXI GCAGC 4 cut(s) 164, 334, 1528, 1540
BsgI GTGCAG 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 962
Bsh1285I CGRYCG 2 cut(s) 370, 1176
BshNI GGYRCC 2 cut(s) 899, 1800
BsiEI CGRYCG 2 cut(s) 370, 1176
BsiHKAI GWGCWC 5 cut(s) 641, 669, 1335, 1406, 2019
BsiHKCI CYCGRG 1 cut(s) 992
BsiSI CCGG 8 cut(s) 309, 366, 722, 813, 993, 1019, 1124, 1761
BsiWI CGTACG 1 cut(s) 531
BslFI GGGAC 5 cut(s) 113, 390, 1444, 1526, 1617
BslI CCNNNNNNNGG 6 cut(s) 220, 294, 706, 801, 1702, 1812
BsmAI GTCTC 3 cut(s) 323, 861, 1383
BsmBI CGTCTC 1 cut(s) 1383
BsmFI GGGAC 5 cut(s) 113, 390, 1444, 1526, 1617
BsoBI CYCGRG 1 cut(s) 992
Bsp1286I GDGCHC 8 cut(s) 207, 641, 669, 881, 1335, 1406, 1805, 2019
Bsp13I TCCGGA 1 cut(s) 1018
Bsp1407I TGTACA 1 cut(s) 1935
Bsp143I GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
Bsp1720I GCTNAGC 1 cut(s) 239
Bsp19I CCATGG 1 cut(s) 552
BspACI CCGC 9 cut(s) 30, 214, 227, 243, 370, 611, 1005, 1082, 1982
BspCNI CTCAG 3 cut(s) 252, 346, 1825
BspEI TCCGGA 1 cut(s) 1018
BspFNI CGCG 1 cut(s) 962
BspHI TCATGA 3 cut(s) 255, 339, 1714
BspOI GCTAGC 1 cut(s) 1326
BspPI GGATC 6 cut(s) 337, 1187, 1415, 1644, 1657, 2053
BspQI GCTCTTC 2 cut(s) 995, 2290
BspT107I GGYRCC 2 cut(s) 899, 1800
BspTI CTTAAG 1 cut(s) 1625
BsrBI CCGCTC 2 cut(s) 229, 1005
BsrDI GCAATG 4 cut(s) 1315, 1345, 2025, 2279
BsrFI RCCGGY 3 cut(s) 365, 812, 1760
BsrGI TGTACA 1 cut(s) 1935
BsrI ACTGG 4 cut(s) 292, 1973, 2033, 2104
BssAI RCCGGY 3 cut(s) 365, 812, 1760
BssECI CCNNGG 7 cut(s) 171, 552, 672, 992, 993, 1227, 1786
BssMI GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
BssNI GRCGYC 2 cut(s) 195, 605
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 3 cut(s) 552, 672, 1786
Bst2BI CACGAG 1 cut(s) 326
Bst2UI CCWGG 2 cut(s) 429, 1946
Bst4CI ACNGT 6 cut(s) 69, 172, 536, 1187, 2143, 2299
Bst6I CTCTTC 2 cut(s) 995, 2290
BstACI GRCGYC 2 cut(s) 195, 605
BstAFI CTTAAG 1 cut(s) 1625
BstAPI GCANNNNNTGC 2 cut(s) 1280, 1550
BstAUI TGTACA 1 cut(s) 1935
BstBAI YACGTR 2 cut(s) 476, 642
BstC8I GCNNGC 5 cut(s) 236, 749, 960, 1254, 1324
BstDEI CTNAG 5 cut(s) 14, 239, 333, 1041, 1833
BstDSI CCRYGG 2 cut(s) 171, 552
BstENI CCTNNNNNAGG 2 cut(s) 704, 1700
BstF5I GGATG 4 cut(s) 652, 1491, 1501, 2137
BstFNI CGCG 1 cut(s) 962
BstHHI GCGC 2 cut(s) 964, 1818
BstKTI GATC 7 cut(s) 345, 1095, 1182, 1423, 1584, 1652, 2061
BstMAI GTCTC 3 cut(s) 323, 861, 1383
BstMBI GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
BstMCI CGRYCG 2 cut(s) 370, 1176
BstNI CCWGG 2 cut(s) 429, 1946
BstNSI RCATGY 4 cut(s) 454, 514, 1519, 2092
BstPAI GACNNNNGTC 1 cut(s) 476
BstSCI CCNGG 5 cut(s) 427, 721, 991, 992, 1944
BstSFI CTRYAG 1 cut(s) 615
BstSLI GKGCMC 3 cut(s) 641, 669, 1805
BstUI CGCG 1 cut(s) 962
BstV1I GCAGC 4 cut(s) 164, 334, 1528, 1540
BstV2I GAAGAC 2 cut(s) 608, 1070
BstX2I RGATCY 2 cut(s) 1420, 1649
BstXI CCANNNNNNTGG 1 cut(s) 559
BstYI RGATCY 2 cut(s) 1420, 1649
BstZI CGGCCG 1 cut(s) 367
BtgI CCRYGG 2 cut(s) 171, 552
BtrI CACGTC 2 cut(s) 319, 517
BtsCI GGATG 4 cut(s) 652, 1491, 1501, 2137
BtsI GCAGTG 2 cut(s) 1333, 2173
BtsIMutI CAGTG 8 cut(s) 120, 299, 487, 1333, 1980, 2040, 2097, 2173
Cac8I GCNNGC 5 cut(s) 236, 749, 960, 1254, 1324
CciI TCATGA 3 cut(s) 255, 339, 1714
CfoI GCGC 2 cut(s) 964, 1818
Cfr10I RCCGGY 3 cut(s) 365, 812, 1760
Cfr13I GGNCC 8 cut(s) 43, 285, 404, 697, 1132, 1459, 1577, 1705
Cfr9I CCCGGG 1 cut(s) 992
CseI GACGC 4 cut(s) 203, 445, 613, 1931
Csp6I GTAC 5 cut(s) 532, 731, 1756, 1936, 2308
CspCI CAANNNNNGTGG 4 cut(s) 1857, 1892, 2083, 2118
CviQI GTAC 5 cut(s) 532, 731, 1756, 1936, 2308
DdeI CTNAG 5 cut(s) 14, 239, 333, 1041, 1833
DpnI GATC 7 cut(s) 344, 1094, 1181, 1422, 1583, 1651, 2060
DpnII GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
DraIII CACNNNGTG 1 cut(s) 294
DrdI GACNNNNNNGTC 1 cut(s) 455
DseDI GACNNNNNNGTC 1 cut(s) 455
EaeI YGGCCR 3 cut(s) 106, 367, 1254
EagI CGGCCG 1 cut(s) 367
Eam1104I CTCTTC 2 cut(s) 995, 2290
EarI CTCTTC 2 cut(s) 995, 2290
EciI GGCGGA 1 cut(s) 600
Ecl136II GAGCTC 1 cut(s) 2017
EclXI CGGCCG 1 cut(s) 367
Eco130I CCWWGG 3 cut(s) 552, 672, 1786
Eco147I AGGCCT 1 cut(s) 1039
Eco24I GRGCYC 3 cut(s) 207, 881, 2019
Eco47I GGWCC 4 cut(s) 285, 404, 1459, 1705
Eco52I CGGCCG 1 cut(s) 367
Eco53kI GAGCTC 1 cut(s) 2017
Eco57I CTGAAG 5 cut(s) 144, 394, 1151, 1979, 1982
Eco72I CACGTG 2 cut(s) 476, 642
Eco88I CYCGRG 1 cut(s) 992
EcoICRI GAGCTC 1 cut(s) 2017
EcoNI CCTNNNNNAGG 2 cut(s) 704, 1700
EcoO109I RGGNCCY 3 cut(s) 404, 697, 1705
EcoRII CCWGG 2 cut(s) 427, 1944
EcoT14I CCWWGG 3 cut(s) 552, 672, 1786
EcoT38I GRGCYC 3 cut(s) 207, 881, 2019
ErhI CCWWGG 3 cut(s) 552, 672, 1786
Esp3I CGTCTC 1 cut(s) 1383
FaqI GGGAC 5 cut(s) 113, 390, 1444, 1526, 1617
FauI CCCGC 1 cut(s) 998
FblI GTMKAC 2 cut(s) 51, 1526
Fnu4HI GCNGC 9 cut(s) 30, 178, 214, 227, 244, 323, 370, 1542, 1554
FokI GGATG 4 cut(s) 659, 1488, 1498, 2144
FriOI GRGCYC 3 cut(s) 207, 881, 2019
Fsp4HI GCNGC 9 cut(s) 30, 178, 214, 227, 244, 323, 370, 1542, 1554
FspBI CTAG 7 cut(s) 701, 882, 1323, 1686, 1769, 2120, 2280
GlaI GCGC 2 cut(s) 963, 1817
GluI GCNGC 9 cut(s) 30, 178, 214, 227, 244, 323, 370, 1542, 1554
GsuI CTGGAG 3 cut(s) 614, 1437, 2016
HapII CCGG 8 cut(s) 309, 366, 722, 813, 993, 1019, 1124, 1761
HgaI GACGC 4 cut(s) 203, 445, 613, 1931
HhaI GCGC 2 cut(s) 964, 1818
Hin1I GRCGYC 2 cut(s) 195, 605
Hin6I GCGC 2 cut(s) 962, 1816
HinP1I GCGC 2 cut(s) 962, 1816
HincII GTYRAC 1 cut(s) 1527
HindII GTYRAC 1 cut(s) 1527
HindIII AAGCTT 6 cut(s) 580, 587, 1260, 1619, 1825, 1961
HinfI GANTC 8 cut(s) 331, 359, 485, 567, 1528, 1630, 1885, 1903
HpaII CCGG 8 cut(s) 309, 366, 722, 813, 993, 1019, 1124, 1761
HphI GGTGA 9 cut(s) 54, 147, 574, 827, 1009, 1523, 2014, 2095, 2147
Hpy188I TCNGA 9 cut(s) 302, 413, 436, 866, 1051, 1228, 1245, 1408, 2194
Hpy99I CGWCG 9 cut(s) 197, 203, 320, 461, 521, 743, 746, 788, 1187
HpyAV CCTTC 7 cut(s) 395, 398, 418, 502, 680, 913, 2155
HpyCH4III ACNGT 6 cut(s) 69, 172, 536, 1187, 2143, 2299
HpyCH4IV ACGT 6 cut(s) 318, 378, 475, 516, 641, 1940
HpyF3I CTNAG 5 cut(s) 14, 239, 333, 1041, 1833
HpySE526I ACGT 6 cut(s) 318, 378, 475, 516, 641, 1940
Hsp92I GRCGYC 2 cut(s) 195, 605
HspAI GCGC 2 cut(s) 962, 1816
Kpn2I TCCGGA 1 cut(s) 1018
Kzo9I GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
LguI GCTCTTC 2 cut(s) 995, 2290
LmnI GCTCC 8 cut(s) 10, 223, 437, 933, 1330, 2022, 2035, 2074
Lsp1109I GCAGC 4 cut(s) 164, 334, 1528, 1540
LweI GCATC 6 cut(s) 255, 637, 1171, 1565, 1652, 2198
MaeI CTAG 7 cut(s) 701, 882, 1323, 1686, 1769, 2120, 2280
MaeII ACGT 6 cut(s) 318, 378, 475, 516, 641, 1940
MaeIII GTNAC 3 cut(s) 761, 1451, 2115
MalI GATC 7 cut(s) 344, 1094, 1181, 1422, 1583, 1651, 2060
MbiI CCGCTC 2 cut(s) 229, 1005
MboI GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
MboII GAAGA 7 cut(s) 613, 800, 1012, 1075, 1157, 1906, 2277
MfeI CAATTG 1 cut(s) 2073
MflI RGATCY 2 cut(s) 1420, 1649
MhlI GDGCHC 8 cut(s) 207, 641, 669, 881, 1335, 1406, 1805, 2019
MlsI TGGCCA 1 cut(s) 1256
MluCI AATT 7 cut(s) 1032, 1917, 2063, 2073, 2109, 2123, 2234
MluNI TGGCCA 1 cut(s) 1256
MlyI GAGTC 6 cut(s) 325, 353, 576, 1522, 1639, 1894
MmeI TCCRAC 6 cut(s) 280, 448, 459, 1029, 1223, 1366
Mox20I TGGCCA 1 cut(s) 1256
MroI TCCGGA 1 cut(s) 1018
MroXI GAANNNNTTC 1 cut(s) 1298
MscI TGGCCA 1 cut(s) 1256
MseI TTAA 4 cut(s) 693, 1626, 1658, 1779
MslI CAYNNNNRTG 3 cut(s) 557, 1338, 1536
Msp20I TGGCCA 1 cut(s) 1256
MspA1I CMGCKG 1 cut(s) 243
MspCI CTTAAG 1 cut(s) 1625
MspI CCGG 8 cut(s) 309, 366, 722, 813, 993, 1019, 1124, 1761
MspR9I CCNGG 5 cut(s) 429, 723, 993, 994, 1946
MunI CAATTG 1 cut(s) 2073
MvaI CCWGG 2 cut(s) 429, 1946
MvnI CGCG 1 cut(s) 962
NciI CCSGG 3 cut(s) 723, 993, 994
NcoI CCATGG 1 cut(s) 552
NdeII GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
NheI GCTAGC 1 cut(s) 1322
NmeAIII GCCGAG 1 cut(s) 210
NmuCI GTSAC 3 cut(s) 761, 1451, 2115
NspI RCATGY 4 cut(s) 454, 514, 1519, 2092
OliI CACNNNNGTG 1 cut(s) 1536
PagI TCATGA 3 cut(s) 255, 339, 1714
PceI AGGCCT 1 cut(s) 1039
PciI ACATGT 2 cut(s) 450, 1515
PciSI GCTCTTC 2 cut(s) 995, 2290
PcsI WCGNNNNNNNCGW 5 cut(s) 198, 324, 522, 647, 1577
PdmI GAANNNNTTC 1 cut(s) 1298
PfeI GAWTC 2 cut(s) 485, 1903
Pfl23II CGTACG 1 cut(s) 531
PflFI GACNNNGTC 1 cut(s) 1187
PflMI CCANNNNNTGG 1 cut(s) 294
PfoI TCCNGGA 2 cut(s) 721, 1944
PkrI GCNGC 9 cut(s) 31, 179, 215, 228, 245, 324, 371, 1543, 1555
PleI GAGTC 6 cut(s) 325, 353, 575, 1522, 1638, 1893
PmaCI CACGTG 2 cut(s) 476, 642
PmlI CACGTG 2 cut(s) 476, 642
PpsI GAGTC 6 cut(s) 325, 353, 575, 1522, 1638, 1893
Ppu21I YACGTR 2 cut(s) 476, 642
PpuMI RGGWCCY 2 cut(s) 404, 1705
PscI ACATGT 2 cut(s) 450, 1515
PshAI GACNNNNGTC 1 cut(s) 476
Psp124BI GAGCTC 1 cut(s) 2019
Psp5II RGGWCCY 2 cut(s) 404, 1705
Psp6I CCWGG 2 cut(s) 427, 1944
PspCI CACGTG 2 cut(s) 476, 642
PspGI CCWGG 2 cut(s) 427, 1944
PspLI CGTACG 1 cut(s) 531
PspPI GGNCC 8 cut(s) 43, 285, 404, 697, 1132, 1459, 1577, 1705
PspPPI RGGWCCY 2 cut(s) 404, 1705
PsuI RGATCY 2 cut(s) 1420, 1649
PsyI GACNNNGTC 1 cut(s) 1187
RsaI GTAC 5 cut(s) 533, 732, 1757, 1937, 2309
RsaNI GTAC 5 cut(s) 532, 731, 1756, 1936, 2308
RseI CAYNNNNRTG 3 cut(s) 557, 1338, 1536
SacI GAGCTC 1 cut(s) 2019
SalI GTCGAC 1 cut(s) 1525
SapI GCTCTTC 2 cut(s) 995, 2290
SaqAI TTAA 4 cut(s) 693, 1626, 1658, 1779
SatI GCNGC 9 cut(s) 30, 178, 214, 227, 244, 323, 370, 1542, 1554
Sau3AI GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
Sau96I GGNCC 8 cut(s) 43, 285, 404, 697, 1132, 1459, 1577, 1705
ScaI AGTACT 1 cut(s) 2309
SchI GAGTC 6 cut(s) 325, 353, 576, 1522, 1639, 1894
ScrFI CCNGG 5 cut(s) 429, 723, 993, 994, 1946
SduI GDGCHC 8 cut(s) 207, 641, 669, 881, 1335, 1406, 1805, 2019
SfaNI GCATC 6 cut(s) 255, 637, 1171, 1565, 1652, 2198
SfcI CTRYAG 1 cut(s) 615
SgrAI CRCCGGYG 1 cut(s) 1760
SinI GGWCC 4 cut(s) 285, 404, 1459, 1705
SmaI CCCGGG 1 cut(s) 994
SmiMI CAYNNNNRTG 3 cut(s) 557, 1338, 1536
SmlI CTYRAG 3 cut(s) 1214, 1625, 1733
SmoI CTYRAG 3 cut(s) 1214, 1625, 1733
Sse9I AATT 7 cut(s) 1032, 1917, 2063, 2073, 2109, 2123, 2234
SseBI AGGCCT 1 cut(s) 1039
SsiI CCGC 9 cut(s) 30, 214, 227, 243, 370, 611, 1005, 1082, 1982
SspMI CTAG 7 cut(s) 701, 882, 1323, 1686, 1769, 2120, 2280
SstI GAGCTC 1 cut(s) 2019
StuI AGGCCT 1 cut(s) 1039
StyD4I CCNGG 5 cut(s) 427, 721, 991, 992, 1944
StyI CCWWGG 3 cut(s) 552, 672, 1786
TaaI ACNGT 6 cut(s) 69, 172, 536, 1187, 2143, 2299
TaiI ACGT 6 cut(s) 321, 381, 478, 519, 644, 1943
TaqI TCGA 9 cut(s) 192, 348, 399, 459, 470, 738, 1182, 1197, 1526
TaqII GACCGA 1 cut(s) 1162
TasI AATT 7 cut(s) 1032, 1917, 2063, 2073, 2109, 2123, 2234
TatI WGTACW 3 cut(s) 1755, 1935, 2307
TauI GCSGC 5 cut(s) 32, 216, 229, 246, 372
TfiI GAWTC 2 cut(s) 485, 1903
Tru1I TTAA 4 cut(s) 693, 1626, 1658, 1779
Tru9I TTAA 4 cut(s) 693, 1626, 1658, 1779
TscAI CASTG 8 cut(s) 127, 299, 487, 1340, 1980, 2040, 2104, 2173
TseFI GTSAC 3 cut(s) 761, 1451, 2115
TseI GCWGC 4 cut(s) 177, 322, 1541, 1553
Tsp45I GTSAC 3 cut(s) 761, 1451, 2115
TspDTI ATGAA 9 cut(s) 172, 244, 328, 812, 1145, 1336, 1486, 1538, 1703
TspGWI ACGGA 1 cut(s) 1837
TspMI CCCGGG 1 cut(s) 992
TspRI CASTG 8 cut(s) 127, 299, 487, 1340, 1980, 2040, 2104, 2173
Tth111I GACNNNGTC 1 cut(s) 1187
Van91I CCANNNNNTGG 1 cut(s) 294
Vha464I CTTAAG 1 cut(s) 1625
VneI GTGCAC 2 cut(s) 637, 665
VpaK11BI GGWCC 4 cut(s) 285, 404, 1459, 1705
XagI CCTNNNNNAGG 2 cut(s) 704, 1700
XapI RAATTY 2 cut(s) 2123, 2234
XceI RCATGY 4 cut(s) 454, 514, 1519, 2092
XmaI CCCGGG 1 cut(s) 992
XmiI GTMKAC 2 cut(s) 51, 1526
XmnI GAANNNNTTC 1 cut(s) 1298
XspI CTAG 7 cut(s) 701, 882, 1323, 1686, 1769, 2120, 2280
ZrmI AGTACT 1 cut(s) 2309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.