Rroxscaffold_2G00079280

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005392
Physical Location & Seq
Forward (+)
2307548 .. 2310594
3047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00079280.1

Sequence Viewer

Length: 2319 bp
ATGGCCCCAAATTTGAGCAATGGCTTTGCCGTCGTTAACGTCGACAATGGCGCAAACCCAAGTCCTTTTAGCCTTCAAGGTTCCAACTTGACGGCCAACGGCCACGTCATCCTCTCCGACGTCCCCCAAAACATAACACTCCGCCCATCACCCCACACCATCAACAAGTCCACCGCCCCCGGCTCTTTTGTTGGTTTCGACGCCGCCGAGGCCAGCAGCCGCCATGTGGTGCCCGTCGGCCAGCTGAAGGACATCCGGTTCATGAGCATCTTCCGGTTCAAGGTCTGGTGGACCACTCACTGGGTCGGATCCAACGGCCGCGACCTCGAGCAGGAGACTCAGATCGTCATCCTCGAAAACTCCGACGCCGGCAGGCCCTACGTCGTCATCCTCCCTCTCCTGGAAGGCGGCTTCCGCGCTTCCATCCAGCCAGGCGCCAAGGACTACCTTGACGTCTGTGTAGAGAGTGGTTCAACGCAGGTCAACGAAAAAGGATTCTCCGGCGTGCTTTATCTTCACGCCGGAGAAGATCCCTTCACGCTGGTGAAGGACGCCGTCAAGGTCGCCAAGGCGCACTTGGGAACGTTCAATCTGTTGGAGGAGAAGACGCCACCTGGTATCGTCGACAAGTTCGGTTGGTGCACATGGGATGCTTTTTACCTCACTGTGCACCCTCGCGGCGTCATTGATGGCGTGAGGAAGCTTGTGGAGGGTGGGTGCCCTCCAGGGCTTGTGTTGCTGGACGACGGGTGGCAGTCCATCGGCCACGACTCTGATCCCATCACCAAAGAAGGGATGGGCCAGACCGCGGCCGGTGAGCAAATGCCGTGCAGGCTGCTCAAGTTCCAAGAGAACTACAAGTTCATAGACTATGTCAGCTCCAAGGACCCTAGTAGCCAAGGCATGGGTGCGTTTGTGAAGGACTTGAAGGACGAGTTCAAGAGCGTTGACTACGTGTACGTGTGGCACGCTCTGTGTGGGTACTGGGGTGGGATCCGGCCCAATGTTCCTGGCCTGCCGGAGGCGGTGGTTGTTGAGCCGAAACTGTCACCGGGGTTGAAGAACACGATGGAAGATCTGGCCGTGGATAAGATAGTCAACACTGGGATTGGGCTGGTCCCACCGGAAATGGTTAACCAGATGTATGAAGGGCTCCACTCTCACCTGAAGTCCGTCGGGATCGACGGCGTCAAGGTCGACGTTATCCATTTGTTGGAAATGTTGTGTGAGAACTATGGTGGCCGTGTGAACCTGGCTAAAGCATATTACGATGCCCTAACAGCTTCAGTGAGGAAGCACTTCGACGGCAATGGTGTTATTGCTAGCATGGAGCACTGTAACGACTTCATGTTCCTTGGAACCGAAGCCATCACTCTCGGCCGTGTCGGTGACGATTTCTGGTGCACTGACCCCTCTGGTGATCCCAACGGTACCTTTTGGTTGCAAGGGTGTCACATGGTTCATTGTGCTTACAATAGCTTATGGATGGGTAACTTCATACAGCCGGATTGGGACATGTTCCAGTCCACTCATCCCTGTGCCGCTTTCCATGCTGCCTCTAGGGCAATCTCCGGGGGTCCTATTTATGTGAGTGACACTGTGGGGAAGCACAACTTTGACTTGCTCAAAAGCCTAGTGGTGCCTGACGGCTCTATCCTTCGCTGCGATTACTATGCTCTCCCTACCCGCGATTGTCTTTTTGAAGATCCTCTCCATGATGGCCGCACCATGCTCAAAATTTGGAACCTCAACAAGTTCAATGGAGTTCTTGGAGCCTTTAACTGCCAAGGAGGAGGCTGGAGCCGTGAGACTAGGCAAAACCAATGTTTTTCTAAATTCGTGGGAAAGGTGACCTCCAATGCCAACCCTAAAGACATTGAGTGGAACAGTGGCAAGAGTCCAATTTCCATTGAAGGAGTGCAAGTGTTTGCCCTGTACTATCACCAGGCCAAGAAGCTAGTACTGTCTAAGCCAACCGAGGATGTGGAATTGTCACTGGAGCCCTTCAACTTTGAGCTAATTGTCGTTTCTCCGGTGACTACTTTGCCCAGCAAGTCTGTCCAATTTGCCCCTATTGGCTTAGTGAACATGCTCAACACCGGCGGTGCTGTCAAGTCGTTGGTTTACAGTGAGGAGGGAAACTCTGTTCAAATTGGGGTGAAGGGCACCGGAGAGATGAGGGTGTTTGCGTCTGAGAAGCCACTGGGTTGCAAGATTGATGGAAGCTGTGTCGCCTTTGAGTATGAAGAGAACATGGTTAGAGTTCATGTGCCATGGACTGGTACCTCCGCCTTGTCTATTGTGGAGTACATATTCTAG

Protein Analysis

772

Amino Acids

84.56

Weight (kDa)

5.47

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 755 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 123, 456
Acc36I ACCTGC 1 cut(s) 469
Acc65I GGTACC 2 cut(s) 1430, 2282
AccB1I GGYRCC 7 cut(s) 229, 434, 717, 1430, 1639, 2165, 2282
AccB7I CCANNNNNTGG 4 cut(s) 300, 904, 1213, 2279
AccI GTMKAC 3 cut(s) 42, 624, 1197
AccII CGCG 5 cut(s) 321, 417, 678, 809, 1689
AclI AACGTT 1 cut(s) 584
AclWI GGATC 9 cut(s) 303, 316, 524, 770, 988, 1001, 1187, 1415, 1700
AcsI RAATTY 3 cut(s) 10, 1737, 1835
AcuI CTGAAG 3 cut(s) 266, 1187, 1269
AcyI GRCGYC 9 cut(s) 120, 201, 366, 435, 453, 552, 608, 681, 1188
AfaI GTAC 7 cut(s) 959, 983, 1432, 1937, 1962, 2284, 2309
AflIII ACRYGT 3 cut(s) 954, 960, 1515
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 7 cut(s) 399, 430, 613, 724, 1009, 1251, 1944
AleI CACNNNNGTG 1 cut(s) 542
AloI GAACNNNNNNTCC 4 cut(s) 242, 274, 2130, 2162
AluBI AGCT 8 cut(s) 244, 703, 879, 1283, 1479, 1957, 2017, 2226
AluI AGCT 8 cut(s) 244, 703, 879, 1283, 1479, 1957, 2017, 2226
Alw21I GWGCWC 4 cut(s) 644, 672, 1335, 1406
Alw26I GTCTC 2 cut(s) 329, 1802
Alw44I GTGCAC 3 cut(s) 640, 668, 1402
AlwI GGATC 9 cut(s) 303, 316, 524, 770, 988, 1001, 1187, 1415, 1700
Ama87I CYCGRG 1 cut(s) 326
ApaLI GTGCAC 3 cut(s) 640, 668, 1402
ApeKI GCWGC 4 cut(s) 216, 835, 1553, 1662
ApoI RAATTY 3 cut(s) 10, 1737, 1835
Asp700I GAANNNNTTC 1 cut(s) 1298
Asp718I GGTACC 2 cut(s) 1430, 2282
AspLEI GCGC 4 cut(s) 53, 419, 437, 574
AspS9I GGNCC 8 cut(s) 4, 291, 375, 799, 886, 999, 1117, 1577
AsuC2I CCSGG 3 cut(s) 180, 1053, 1573
AsuNHI GCTAGC 1 cut(s) 1322
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 4 cut(s) 291, 886, 1117, 1577
BaeGI GKGCMC 6 cut(s) 234, 644, 672, 722, 1406, 2168
BaeI ACNNNNGTAYC 2 cut(s) 973, 1006
BamHI GGATCC 2 cut(s) 308, 993
BanI GGYRCC 7 cut(s) 229, 434, 717, 1430, 1639, 2165, 2282
BanII GRGCYC 2 cut(s) 1155, 2004
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 4 cut(s) 644, 672, 1335, 1406
BbvI GCAGC 4 cut(s) 228, 822, 1540, 1649
BcgI CGANNNNNNTGC 2 cut(s) 1655, 1689
BciT130I CCWGG 7 cut(s) 401, 432, 615, 726, 1011, 1253, 1946
BcnI CCSGG 3 cut(s) 180, 1053, 1573
BcoDI GTCTC 2 cut(s) 329, 1802
BfaI CTAG 7 cut(s) 891, 1323, 1560, 1634, 1812, 1958, 2317
BfoI RGCGCY 1 cut(s) 438
BfuAI ACCTGC 1 cut(s) 469
BglI GCCNNNNNGGC 3 cut(s) 209, 832, 1562
BglII AGATCT 1 cut(s) 1075
BmcAI AGTACT 1 cut(s) 1962
Bme18I GGWCC 4 cut(s) 291, 886, 1117, 1577
BmeT110I CYCGRG 1 cut(s) 326
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 8 cut(s) 4, 291, 375, 799, 886, 999, 1117, 1577
BmrI ACTGGG 4 cut(s) 310, 994, 1113, 2213
BmsI GCATC 3 cut(s) 276, 640, 1261
BmtI GCTAGC 1 cut(s) 1326
BmuI ACTGGG 4 cut(s) 310, 994, 1113, 2213
BpiI GAAGAC 1 cut(s) 611
BplI GAGNNNNNCTC 2 cut(s) 2126, 2158
BpmI CTGGAG 3 cut(s) 708, 1819, 2018
BpuEI CTTGAG 1 cut(s) 824
BpuMI CCSGG 3 cut(s) 180, 1053, 1573
BsaAI YACGTR 2 cut(s) 955, 961
BsaBI GATNNNNATC 1 cut(s) 347
BsaHI GRCGYC 9 cut(s) 120, 201, 366, 435, 453, 552, 608, 681, 1188
BsaWI WCCGGW 5 cut(s) 255, 273, 1123, 2032, 2168
BsaXI ACNNNNNCTCC 4 cut(s) 701, 731, 1908, 1938
Bse118I RCCGGY 3 cut(s) 368, 812, 2099
Bse1I ACTGG 7 cut(s) 305, 989, 1108, 1522, 2001, 2208, 2284
Bse3DI GCAATG 2 cut(s) 25, 1315
Bse8I GATNNNNATC 1 cut(s) 347
BseBI CCWGG 7 cut(s) 401, 432, 615, 726, 1011, 1253, 1946
BseJI GATNNNNATC 1 cut(s) 347
BseMI GCAATG 2 cut(s) 25, 1315
BseMII CTCAG 2 cut(s) 353, 2184
BseNI ACTGG 7 cut(s) 305, 989, 1108, 1522, 2001, 2208, 2284
BseRI GAGGAG 3 cut(s) 614, 1806, 2147
BseSI GKGCMC 6 cut(s) 234, 644, 672, 722, 1406, 2168
BseX3I CGGCCG 3 cut(s) 316, 810, 1378
BseXI GCAGC 4 cut(s) 228, 822, 1540, 1649
BseYI CCCAGC 1 cut(s) 2048
BsgI GTGCAG 1 cut(s) 850
Bsh1236I CGCG 5 cut(s) 321, 417, 678, 809, 1689
Bsh1285I CGRYCG 3 cut(s) 319, 813, 1381
BshNI GGYRCC 7 cut(s) 229, 434, 717, 1430, 1639, 2165, 2282
BsiEI CGRYCG 3 cut(s) 319, 813, 1381
BsiHKAI GWGCWC 4 cut(s) 644, 672, 1335, 1406
BsiHKCI CYCGRG 1 cut(s) 326
BslFI GGGAC 3 cut(s) 107, 1103, 1526
BsmAI GTCTC 2 cut(s) 329, 1802
BsmFI GGGAC 3 cut(s) 107, 1103, 1526
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 9 cut(s) 234, 644, 672, 722, 1155, 1335, 1406, 2004, 2168
Bsp143I GATC 9 cut(s) 308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
Bsp19I CCATGG 1 cut(s) 2273
BspCNI CTCAG 2 cut(s) 352, 2185
BspFNI CGCG 5 cut(s) 321, 417, 678, 809, 1689
BspHI TCATGA 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 469
BspOI GCTAGC 1 cut(s) 1326
BspPI GGATC 9 cut(s) 303, 316, 524, 770, 988, 1001, 1187, 1415, 1700
BspT107I GGYRCC 7 cut(s) 229, 434, 717, 1430, 1639, 2165, 2282
BsrDI GCAATG 2 cut(s) 25, 1315
BsrFI RCCGGY 3 cut(s) 368, 812, 2099
BsrI ACTGG 7 cut(s) 305, 989, 1108, 1522, 2001, 2208, 2284
BssAI RCCGGY 3 cut(s) 368, 812, 2099
BssMI GATC 9 cut(s) 308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
BssNI GRCGYC 9 cut(s) 120, 201, 366, 435, 453, 552, 608, 681, 1188
BssT1I CCWWGG 7 cut(s) 438, 567, 882, 898, 1354, 1786, 2273
Bst2UI CCWGG 7 cut(s) 401, 432, 615, 726, 1011, 1253, 1946
Bst4CI ACNGT 8 cut(s) 667, 1047, 1337, 1430, 1600, 1889, 1965, 2129
Bst6I CTCTTC 1 cut(s) 2241
BstACI GRCGYC 9 cut(s) 120, 201, 366, 435, 453, 552, 608, 681, 1188
BstBAI YACGTR 2 cut(s) 955, 961
BstC8I GCNNGC 9 cut(s) 214, 242, 370, 374, 506, 833, 969, 1016, 1324
BstDEI CTNAG 4 cut(s) 339, 1968, 2080, 2193
BstDSI CCRYGG 3 cut(s) 807, 1083, 2273
BstEII GGTNACC 1 cut(s) 1849
BstENI CCTNNNNNAGG 2 cut(s) 329, 1019
BstFNI CGCG 5 cut(s) 321, 417, 678, 809, 1689
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 4 cut(s) 53, 419, 437, 574
BstKTI GATC 9 cut(s) 311, 345, 532, 778, 996, 1078, 1182, 1423, 1708
BstMAI GTCTC 2 cut(s) 329, 1802
BstMBI GATC 9 cut(s) 308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
BstMCI CGRYCG 3 cut(s) 319, 813, 1381
BstMWI GCNNNNNNNGC 7 cut(s) 209, 414, 736, 832, 1280, 1550, 1562
BstNI CCWGG 7 cut(s) 401, 432, 615, 726, 1011, 1253, 1946
BstNSI RCATGY 2 cut(s) 1519, 2092
BstPI GGTNACC 1 cut(s) 1849
BstSLI GKGCMC 6 cut(s) 234, 644, 672, 722, 1406, 2168
BstUI CGCG 5 cut(s) 321, 417, 678, 809, 1689
BstV1I GCAGC 4 cut(s) 228, 822, 1540, 1649
BstV2I GAAGAC 1 cut(s) 611
BstX2I RGATCY 5 cut(s) 308, 529, 993, 1075, 1705
BstYI RGATCY 5 cut(s) 308, 529, 993, 1075, 1705
BstZI CGGCCG 3 cut(s) 316, 810, 1378
BtgI CCRYGG 3 cut(s) 807, 1083, 2273
BtrI CACGTC 1 cut(s) 106
BveI ACCTGC 1 cut(s) 469
Cac8I GCNNGC 9 cut(s) 214, 242, 370, 374, 506, 833, 969, 1016, 1324
CciI TCATGA 1 cut(s) 261
CfoI GCGC 4 cut(s) 53, 419, 437, 574
Cfr10I RCCGGY 3 cut(s) 368, 812, 2099
Cfr13I GGNCC 8 cut(s) 4, 291, 375, 799, 886, 999, 1117, 1577
Cfr42I CCGCGG 1 cut(s) 810
CseI GACGC 7 cut(s) 209, 374, 560, 616, 670, 1177, 2178
CsiI ACCWGGT 1 cut(s) 613
Csp6I GTAC 7 cut(s) 958, 982, 1431, 1936, 1961, 2283, 2308
CviQI GTAC 7 cut(s) 958, 982, 1431, 1936, 1961, 2283, 2308
DdeI CTNAG 4 cut(s) 339, 1968, 2080, 2193
DinI GGCGCC 1 cut(s) 436
DpnI GATC 9 cut(s) 310, 344, 531, 777, 995, 1077, 1181, 1422, 1707
DpnII GATC 9 cut(s) 308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
EagI CGGCCG 3 cut(s) 316, 810, 1378
Eam1104I CTCTTC 1 cut(s) 2241
EarI CTCTTC 1 cut(s) 2241
EciI GGCGGA 2 cut(s) 131, 2278
EclXI CGGCCG 3 cut(s) 316, 810, 1378
Eco130I CCWWGG 7 cut(s) 438, 567, 882, 898, 1354, 1786, 2273
Eco24I GRGCYC 2 cut(s) 1155, 2004
Eco47I GGWCC 4 cut(s) 291, 886, 1117, 1577
Eco52I CGGCCG 3 cut(s) 316, 810, 1378
Eco57I CTGAAG 3 cut(s) 266, 1187, 1269
Eco88I CYCGRG 1 cut(s) 326
Eco91I GGTNACC 1 cut(s) 1849
EcoNI CCTNNNNNAGG 2 cut(s) 329, 1019
EcoO109I RGGNCCY 3 cut(s) 375, 886, 1577
EcoO65I GGTNACC 1 cut(s) 1849
EcoRII CCWGG 7 cut(s) 399, 430, 613, 724, 1009, 1251, 1944
EcoT14I CCWWGG 7 cut(s) 438, 567, 882, 898, 1354, 1786, 2273
EcoT38I GRGCYC 2 cut(s) 1155, 2004
EgeI GGCGCC 1 cut(s) 436
EheI GGCGCC 1 cut(s) 436
ErhI CCWWGG 7 cut(s) 438, 567, 882, 898, 1354, 1786, 2273
FalI AAGNNNNNCTT 4 cut(s) 560, 592, 1598, 1630
FaqI GGGAC 3 cut(s) 107, 1103, 1526
FauI CCCGC 1 cut(s) 1694
FblI GTMKAC 3 cut(s) 42, 624, 1197
FriOI GRGCYC 2 cut(s) 1155, 2004
FspBI CTAG 7 cut(s) 891, 1323, 1560, 1634, 1812, 1958, 2317
GlaI GCGC 4 cut(s) 52, 418, 436, 573
GsaI CCCAGC 1 cut(s) 2052
GsuI CTGGAG 3 cut(s) 708, 1819, 2018
HaeII RGCGCY 1 cut(s) 438
HgaI GACGC 7 cut(s) 209, 374, 560, 616, 670, 1177, 2178
HhaI GCGC 4 cut(s) 53, 419, 437, 574
Hin1I GRCGYC 9 cut(s) 120, 201, 366, 435, 453, 552, 608, 681, 1188
Hin6I GCGC 4 cut(s) 51, 417, 435, 572
HinP1I GCGC 4 cut(s) 51, 417, 435, 572
HincII GTYRAC 8 cut(s) 37, 43, 484, 625, 949, 1099, 1135, 1198
HindII GTYRAC 8 cut(s) 37, 43, 484, 625, 949, 1099, 1135, 1198
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 4 cut(s) 337, 495, 770, 1897
HpaI GTTAAC 2 cut(s) 37, 1135
Hpy188I TCNGA 6 cut(s) 118, 308, 342, 364, 775, 2194
Hpy188III TCNNGA 3 cut(s) 262, 940, 1177
HpyCH4III ACNGT 8 cut(s) 667, 1047, 1337, 1430, 1600, 1889, 1965, 2129
HpyCH4IV ACGT 9 cut(s) 39, 105, 120, 381, 453, 584, 954, 960, 1200
HpyCH4V TGCA 7 cut(s) 642, 670, 831, 1404, 1444, 1921, 2211
HpyF10VI GCNNNNNNNGC 7 cut(s) 209, 414, 736, 832, 1280, 1550, 1562
HpyF3I CTNAG 4 cut(s) 339, 1968, 2080, 2193
HpySE526I ACGT 9 cut(s) 39, 105, 120, 381, 453, 584, 954, 960, 1200
Hsp92I GRCGYC 9 cut(s) 120, 201, 366, 435, 453, 552, 608, 681, 1188
HspAI GCGC 4 cut(s) 51, 417, 435, 572
KasI GGCGCC 1 cut(s) 434
KpnI GGTACC 2 cut(s) 1434, 2286
KroI GCCGGC 1 cut(s) 368
KroNI GCCGGC 1 cut(s) 370
KspAI GTTAAC 2 cut(s) 37, 1135
KspI CCGCGG 1 cut(s) 810
Kzo9I GATC 9 cut(s) 308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
LmnI GCTCC 6 cut(s) 884, 1158, 1330, 1772, 1800, 1999
Lsp1109I GCAGC 4 cut(s) 228, 822, 1540, 1649
LweI GCATC 3 cut(s) 276, 640, 1261
MabI ACCWGGT 1 cut(s) 613
MaeI CTAG 7 cut(s) 891, 1323, 1560, 1634, 1812, 1958, 2317
MaeII ACGT 9 cut(s) 39, 105, 120, 381, 453, 584, 954, 960, 1200
MaeIII GTNAC 9 cut(s) 1047, 1337, 1388, 1451, 1490, 1592, 1849, 1992, 2035
MalI GATC 9 cut(s) 310, 344, 531, 777, 995, 1077, 1181, 1422, 1707
MboI GATC 9 cut(s) 308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
MboII GAAGA 8 cut(s) 262, 506, 539, 616, 1072, 1085, 1715, 2258
MflI RGATCY 5 cut(s) 308, 529, 993, 1075, 1705
MhlI GDGCHC 9 cut(s) 234, 644, 672, 722, 1155, 1335, 1406, 2004, 2168
MluCI AATT 8 cut(s) 10, 1737, 1835, 1902, 1988, 2019, 2063, 2151
Mly113I GGCGCC 1 cut(s) 435
MlyI GAGTC 3 cut(s) 331, 764, 1906
MmeI TCCRAC 7 cut(s) 108, 141, 286, 336, 387, 576, 1194
MroNI GCCGGC 1 cut(s) 368
MroXI GAANNNNTTC 1 cut(s) 1298
MseI TTAA 3 cut(s) 36, 1134, 1779
MslI CAYNNNNRTG 2 cut(s) 542, 1536
MspA1I CMGCKG 2 cut(s) 244, 809
MvaI CCWGG 7 cut(s) 401, 432, 615, 726, 1011, 1253, 1946
MvnI CGCG 5 cut(s) 321, 417, 678, 809, 1689
MwoI GCNNNNNNNGC 7 cut(s) 209, 414, 736, 832, 1280, 1550, 1562
NaeI GCCGGC 1 cut(s) 370
NarI GGCGCC 1 cut(s) 435
NciI CCSGG 3 cut(s) 180, 1053, 1573
NcoI CCATGG 1 cut(s) 2273
NdeII GATC 9 cut(s) 308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
NgoMIV GCCGGC 1 cut(s) 368
NheI GCTAGC 1 cut(s) 1322
NmeAIII GCCGAG 2 cut(s) 232, 1356
NmuCI GTSAC 7 cut(s) 1047, 1388, 1451, 1592, 1849, 1992, 2035
NspI RCATGY 2 cut(s) 1519, 2092
OliI CACNNNNGTG 1 cut(s) 542
PaeR7I CTCGAG 1 cut(s) 326
PagI TCATGA 1 cut(s) 261
PciI ACATGT 1 cut(s) 1515
PcsI WCGNNNNNNNCGW 4 cut(s) 39, 204, 351, 360
PdiI GCCGGC 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 1298
PfeI GAWTC 1 cut(s) 495
PflFI GACNNNGTC 3 cut(s) 554, 872, 1187
PflMI CCANNNNNTGG 4 cut(s) 300, 904, 1213, 2279
PfoI TCCNGGA 1 cut(s) 399
PleI GAGTC 3 cut(s) 331, 764, 1905
PluTI GGCGCC 1 cut(s) 438
PpsI GAGTC 3 cut(s) 331, 764, 1905
Ppu21I YACGTR 2 cut(s) 955, 961
PpuMI RGGWCCY 2 cut(s) 886, 1577
PscI ACATGT 1 cut(s) 1515
Psp1406I AACGTT 1 cut(s) 584
Psp5II RGGWCCY 2 cut(s) 886, 1577
Psp6I CCWGG 7 cut(s) 399, 430, 613, 724, 1009, 1251, 1944
PspEI GGTNACC 1 cut(s) 1849
PspFI CCCAGC 1 cut(s) 2048
PspGI CCWGG 7 cut(s) 399, 430, 613, 724, 1009, 1251, 1944
PspPI GGNCC 8 cut(s) 4, 291, 375, 799, 886, 999, 1117, 1577
PspPPI RGGWCCY 2 cut(s) 886, 1577
PspXI VCTCGAGB 1 cut(s) 326
PsuI RGATCY 5 cut(s) 308, 529, 993, 1075, 1705
PsyI GACNNNGTC 3 cut(s) 554, 872, 1187
PvuII CAGCTG 1 cut(s) 244
RsaI GTAC 7 cut(s) 959, 983, 1432, 1937, 1962, 2284, 2309
RsaNI GTAC 7 cut(s) 958, 982, 1431, 1936, 1961, 2283, 2308
RseI CAYNNNNRTG 2 cut(s) 542, 1536
SacII CCGCGG 1 cut(s) 810
SalI GTCGAC 3 cut(s) 41, 623, 1196
SaqAI TTAA 3 cut(s) 36, 1134, 1779
Sau3AI GATC 9 cut(s) 308, 342, 529, 775, 993, 1075, 1179, 1420, 1705
Sau96I GGNCC 8 cut(s) 4, 291, 375, 799, 886, 999, 1117, 1577
ScaI AGTACT 1 cut(s) 1962
SchI GAGTC 3 cut(s) 331, 764, 1906
SduI GDGCHC 9 cut(s) 234, 644, 672, 722, 1155, 1335, 1406, 2004, 2168
SexAI ACCWGGT 1 cut(s) 613
SfaNI GCATC 3 cut(s) 276, 640, 1261
SfoI GGCGCC 1 cut(s) 436
Sfr274I CTCGAG 1 cut(s) 326
Sfr303I CCGCGG 1 cut(s) 810
SgrAI CRCCGGYG 1 cut(s) 2099
SgrBI CCGCGG 1 cut(s) 810
SinI GGWCC 4 cut(s) 291, 886, 1117, 1577
SlaI CTCGAG 1 cut(s) 326
SmiMI CAYNNNNRTG 2 cut(s) 542, 1536
SmlI CTYRAG 2 cut(s) 326, 839
SmoI CTYRAG 2 cut(s) 326, 839
Sse9I AATT 8 cut(s) 10, 1737, 1835, 1902, 1988, 2019, 2063, 2151
SspDI GGCGCC 1 cut(s) 434
SspMI CTAG 7 cut(s) 891, 1323, 1560, 1634, 1812, 1958, 2317
StyI CCWWGG 7 cut(s) 438, 567, 882, 898, 1354, 1786, 2273
TaaI ACNGT 8 cut(s) 667, 1047, 1337, 1430, 1600, 1889, 1965, 2129
TaiI ACGT 9 cut(s) 42, 108, 123, 384, 456, 587, 957, 963, 1203
TaqI TCGA 8 cut(s) 42, 198, 327, 354, 624, 1182, 1197, 1302
TasI AATT 8 cut(s) 10, 1737, 1835, 1902, 1988, 2019, 2063, 2151
TatI WGTACW 3 cut(s) 1935, 1960, 2307
TauI GCSGC 8 cut(s) 206, 222, 321, 411, 681, 812, 1544, 1725
TfiI GAWTC 1 cut(s) 495
Tru1I TTAA 3 cut(s) 36, 1134, 1779
Tru9I TTAA 3 cut(s) 36, 1134, 1779
TseFI GTSAC 7 cut(s) 1047, 1388, 1451, 1592, 1849, 1992, 2035
TseI GCWGC 4 cut(s) 216, 835, 1553, 1662
Tsp45I GTSAC 7 cut(s) 1047, 1388, 1451, 1592, 1849, 1992, 2035
TspDTI ATGAA 8 cut(s) 250, 853, 1161, 1336, 1451, 1486, 2255, 2259
TspGWI ACGGA 1 cut(s) 1162
Tth111I GACNNNGTC 3 cut(s) 554, 872, 1187
Van91I CCANNNNNTGG 4 cut(s) 300, 904, 1213, 2279
VneI GTGCAC 3 cut(s) 640, 668, 1402
VpaK11BI GGWCC 4 cut(s) 291, 886, 1117, 1577
XagI CCTNNNNNAGG 2 cut(s) 329, 1019
XapI RAATTY 3 cut(s) 10, 1737, 1835
XceI RCATGY 2 cut(s) 1519, 2092
XcmI CCANNNNNNNNNTGG 2 cut(s) 574, 793
XhoI CTCGAG 1 cut(s) 326
XmiI GTMKAC 3 cut(s) 42, 624, 1197
XmnI GAANNNNTTC 1 cut(s) 1298
XspI CTAG 7 cut(s) 891, 1323, 1560, 1634, 1812, 1958, 2317
ZraI GACGTC 2 cut(s) 121, 454
ZrmI AGTACT 1 cut(s) 1962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.