Rh2AG644700

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005392
Physical Location & Seq
Reverse (-)
86604594 .. 86607738
3145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG644700.1

Sequence Viewer

Length: 2310 bp
ATGGCCCCAAATTTGAGCAATGGCTTTGCCGTCGACAATGGCGCAAACCCAAGTCCTTTTAGCCTTCAAGGTTCCAACTTGACTGCCAACGGCCACGTCATCCTCTCCGACGTCCCCCAAAACATAACACTCCACCCATCACCCCACACCATCAACAAGTCCACCACCCCCGGCTCTTTTGTTGGCTTCGACGCCGCCGAGGCCAGCAGCCGCCACGTGGTGCCCGTCGGCCAGCTGAAGGACATCCGGTTCATGAGCATCTTCCGGTTCAAGGTCTGGTGGACCACTCACTGGGTCGGATCCAACGGCCGCGACCTCGAGCAGGAGACTCAGATCGTCATCCTCGAAAACTCCGACGCCGGCAGGCCCTACGTCGTCATCCTCCCGCTCCTTGAAGGCGGCTTCCGCGCTTCCATCCAGCCAGGCTCCGAGGACTACCTCGACGTCTGTGTAGAGAGCGGTTCAACGCAGGTCAACGAAAAAGGATTCTCCGGCGTGCTTTATCTTCACGCCGGAGAAGATCCCTTCACGCTGGTGAAGGACGCCGTCAAGGTCGCCAAGGCGCACTTGGGAACGTTCAATCTGTTGGAGGAGAAGACGCCACCTGGCATCGTCGACAAGTTCGGTTGGTGCACATGGGATGCTTTTTACCTCACTGTGCACCCTCGCGGCGTCATTGATGGCGTGAGGAAGCTTGTGGAGGGTGGGTGCCCTCCGGGGCTTGTGTTGCTGGACGACGGGTGGCAGTCCATAGGCCACGACTCTGATCCCATCACCAAAGAAGGGATGGGCCAGACCGCCGCCGGTGAGCAAATGCCGTGCAGGCTGCTCAAGTTCCAAGAGAACTACAAGTTCATAGACTATGTCAGCTCCAAGGACCATAGTAGCCGAGGCATGGGTGCGTTTGTGAAGGACTTGAAGGACGAGTTCAAGAGCGTTGACTACGTGTACGTGTGGCACGCTCTGTGTGGGTACTGGGGTGGGATCCGGCCCAATGTTCCTGGCCTGCCGGAGGCGGTGGTTGTTAGGCCGGAACTGTCACCGGGGTTGAAGAACACGATGGAAGATCTGGCCGTGGATAAGATAGTCAACACTGGGATTGGGCTGGTCCCACCGGAAATGGTTAACCAGATGTATGAAGGGCTCCACTCTCACCTGAAGTCCGTCGGGATAGACGGCGTCAAGGTCGACGTTATCCATTTGTTGGAAATGTTGTGTGAGAACTATGGTGGCCGTGTGAACCTGGCTAAAGCATATTACGATGCCCTAACAGCTTCAGTGAGGAAGCACTTCGACGGCAATGGTGTCATTGCTAGCATGGAGCACTGTAACGACTTCATGTTCCTTGGAACCGAAGCCATCACTCTCGGCCGTGTCGGTGACGATTTCTGGTGCACTGACCCCTCTGGTGATCCCAACGGCACCTTTTGGTTGCAAGGGTGTCACATGGTTCATTGTGCTTACAATAGCTTATGGATGGGTAACTTCATACAGCCGGATTGGGACATGTTCCAGTCCACTCATCCCTGTGCCGCTTTCCATGCTGCCTCTAGGGCAATCTCCGGCGGTCCTATTTATGTGAGTGACACTGTGGGGAAGCACAACTTTGACTTGCTCAAAACCCTAGTGGTGCCTGACGGCTCTATCCTTCGCTGCGAGTACTATGCTCTCCCTACCCGCGATTGTCTTTTTGAGGATCCTCTCCATGATGGCCGCACCATGCTCAAAATTTGGAACCTCAACAAGTTCAATGGAGTTCTCGGAGCCTTTAACTGCCAAGGAGGAGGCTGGAGCCCAGAGACTAGGCGAAACCAATGTTTTTCTAAATTCGTGGGAAAGGTGACCTCCAAGGCCAACCCTAAAGACATTGAGTGGAACAGTGGCAAGAGTACAATTTCCATTGAAGGAGTGCAAGTGTTTGCCTTGTACTATCACCAGGCCAAGAAGCTAGTCCTGTCTAAGCCAACCGAGGATGTGGAGTTGTCACTGGAGCCCTTCAACTTTGAGCTAATTGTCGTTTCTCCGGTGACTACTTTGCCTAGCAAGTCTGTCCAATTTGCCCCTATTGGCTTAGTGAACATGCTCAACACCGGCGGTGCTGTCAAGTCGTTGGTTTACAGTGAGGAGGGAGACTCTGTTCAAATTGGGGTGAAGGGCACCGGAGAGATGAGGGTGTTTGCGTCTGAGAAGCCACTGGGTTGCAAGATTGATGGAAGCTGTGTCGCTTTTGAGTATGAAGAGAACATGGTTAGAGTTCATGTGCCATGGACTGGTACATCCACCTTGTCTATTGTGGAGTACATATTCTAG

Protein Analysis

769

Amino Acids

84.42

Weight (kDa)

5.45

Isoelectric Point (pI)

29.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 21 - 752 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 114, 447
Acc36I ACCTGC 1 cut(s) 460
AccB1I GGYRCC 5 cut(s) 220, 708, 1421, 1630, 2156
AccB7I CCANNNNNTGG 3 cut(s) 291, 1204, 2270
AccBSI CCGCTC 2 cut(s) 388, 459
AccI GTMKAC 3 cut(s) 33, 615, 1188
AccII CGCG 4 cut(s) 312, 408, 669, 1680
AclI AACGTT 1 cut(s) 575
AclWI GGATC 9 cut(s) 294, 307, 515, 761, 979, 992, 1406, 1691, 1704
AcoI YGGCCR 7 cut(s) 91, 229, 307, 1071, 1231, 1369, 1711
AcsI RAATTY 3 cut(s) 10, 1728, 1826
AcuI CTGAAG 3 cut(s) 257, 1178, 1260
AcvI CACGTG 1 cut(s) 217
AcyI GRCGYC 8 cut(s) 111, 192, 357, 444, 543, 599, 672, 1179
AdeI CACNNNGTG 1 cut(s) 220
AfaI GTAC 7 cut(s) 950, 974, 1661, 1891, 1928, 2275, 2300
AflIII ACRYGT 3 cut(s) 945, 951, 1506
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 5 cut(s) 421, 604, 1000, 1242, 1935
AleI CACNNNNGTG 1 cut(s) 533
AloI GAACNNNNNNTCC 4 cut(s) 233, 265, 2121, 2153
AluBI AGCT 8 cut(s) 235, 694, 870, 1274, 1470, 1948, 2008, 2217
AluI AGCT 8 cut(s) 235, 694, 870, 1274, 1470, 1948, 2008, 2217
Alw21I GWGCWC 4 cut(s) 635, 663, 1326, 1397
Alw26I GTCTC 3 cut(s) 320, 1793, 2124
Alw44I GTGCAC 3 cut(s) 631, 659, 1393
AlwI GGATC 9 cut(s) 294, 307, 515, 761, 979, 992, 1406, 1691, 1704
Ama87I CYCGRG 1 cut(s) 317
ApaLI GTGCAC 3 cut(s) 631, 659, 1393
ApeKI GCWGC 4 cut(s) 207, 826, 1544, 1653
ApoI RAATTY 3 cut(s) 10, 1728, 1826
Asp700I GAANNNNTTC 1 cut(s) 1289
AspLEI GCGC 3 cut(s) 44, 410, 565
AspS9I GGNCC 8 cut(s) 4, 282, 366, 790, 877, 990, 1108, 1568
AsuC2I CCSGG 3 cut(s) 171, 717, 1044
AsuNHI GCTAGC 1 cut(s) 1313
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 4 cut(s) 282, 877, 1108, 1568
BaeGI GKGCMC 6 cut(s) 225, 635, 663, 713, 1397, 2159
BaeI ACNNNNGTAYC 2 cut(s) 964, 997
BamHI GGATCC 3 cut(s) 299, 984, 1696
BanI GGYRCC 5 cut(s) 220, 708, 1421, 1630, 2156
BanII GRGCYC 3 cut(s) 1146, 1796, 1995
BbrPI CACGTG 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 602
Bbv12I GWGCWC 4 cut(s) 635, 663, 1326, 1397
BbvI GCAGC 4 cut(s) 219, 813, 1531, 1640
BcgI CGANNNNNNTGC 2 cut(s) 1646, 1680
BciT130I CCWGG 5 cut(s) 423, 606, 1002, 1244, 1937
BcnI CCSGG 3 cut(s) 171, 717, 1044
BcoDI GTCTC 3 cut(s) 320, 1793, 2124
BfaI CTAG 7 cut(s) 1314, 1551, 1625, 1803, 1949, 2040, 2308
BfuAI ACCTGC 1 cut(s) 460
BglI GCCNNNNNGGC 3 cut(s) 200, 823, 1553
BglII AGATCT 1 cut(s) 1066
BmcAI AGTACT 1 cut(s) 1661
Bme1390I CCNGG 8 cut(s) 171, 423, 606, 717, 1002, 1044, 1244, 1937
Bme18I GGWCC 4 cut(s) 282, 877, 1108, 1568
BmeT110I CYCGRG 1 cut(s) 317
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 8 cut(s) 4, 282, 366, 790, 877, 990, 1108, 1568
BmrFI CCNGG 8 cut(s) 171, 423, 606, 717, 1002, 1044, 1244, 1937
BmrI ACTGGG 4 cut(s) 301, 985, 1104, 2204
BmsI GCATC 4 cut(s) 267, 618, 631, 1252
BmtI GCTAGC 1 cut(s) 1317
BmuI ACTGGG 4 cut(s) 301, 985, 1104, 2204
BpiI GAAGAC 1 cut(s) 602
BplI GAGNNNNNCTC 2 cut(s) 2117, 2149
BpmI CTGGAG 2 cut(s) 1810, 2009
BpuEI CTTGAG 1 cut(s) 815
BpuMI CCSGG 3 cut(s) 171, 717, 1044
BsaAI YACGTR 3 cut(s) 217, 946, 952
BsaBI GATNNNNATC 1 cut(s) 338
BsaHI GRCGYC 8 cut(s) 111, 192, 357, 444, 543, 599, 672, 1179
BsaWI WCCGGW 5 cut(s) 246, 264, 1114, 2023, 2159
BsaXI ACNNNNNCTCC 6 cut(s) 692, 722, 1899, 1929, 2121, 2151
Bse118I RCCGGY 3 cut(s) 359, 803, 2090
Bse1I ACTGG 7 cut(s) 296, 980, 1099, 1513, 1992, 2199, 2275
Bse3DI GCAATG 3 cut(s) 25, 1306, 1308
Bse8I GATNNNNATC 1 cut(s) 338
BseBI CCWGG 5 cut(s) 423, 606, 1002, 1244, 1937
BseJI GATNNNNATC 1 cut(s) 338
BseMI GCAATG 3 cut(s) 25, 1306, 1308
BseMII CTCAG 2 cut(s) 344, 2175
BseNI ACTGG 7 cut(s) 296, 980, 1099, 1513, 1992, 2199, 2275
BseRI GAGGAG 3 cut(s) 605, 1797, 2138
BseSI GKGCMC 6 cut(s) 225, 635, 663, 713, 1397, 2159
BseX3I CGGCCG 2 cut(s) 307, 1369
BseXI GCAGC 4 cut(s) 219, 813, 1531, 1640
BsgI GTGCAG 1 cut(s) 841
Bsh1236I CGCG 4 cut(s) 312, 408, 669, 1680
Bsh1285I CGRYCG 2 cut(s) 310, 1372
BshNI GGYRCC 5 cut(s) 220, 708, 1421, 1630, 2156
BsiEI CGRYCG 2 cut(s) 310, 1372
BsiHKAI GWGCWC 4 cut(s) 635, 663, 1326, 1397
BsiHKCI CYCGRG 1 cut(s) 317
BslFI GGGAC 3 cut(s) 98, 1094, 1517
BsmAI GTCTC 3 cut(s) 320, 1793, 2124
BsmFI GGGAC 3 cut(s) 98, 1094, 1517
BsoBI CYCGRG 1 cut(s) 317
Bsp143I GATC 8 cut(s) 299, 333, 520, 766, 984, 1066, 1411, 1696
Bsp19I CCATGG 1 cut(s) 2264
BspCNI CTCAG 2 cut(s) 343, 2176
BspFNI CGCG 4 cut(s) 312, 408, 669, 1680
BspHI TCATGA 1 cut(s) 252
BspMI ACCTGC 1 cut(s) 460
BspOI GCTAGC 1 cut(s) 1317
BspPI GGATC 9 cut(s) 294, 307, 515, 761, 979, 992, 1406, 1691, 1704
BspT107I GGYRCC 5 cut(s) 220, 708, 1421, 1630, 2156
BsrBI CCGCTC 2 cut(s) 388, 459
BsrDI GCAATG 3 cut(s) 25, 1306, 1308
BsrFI RCCGGY 3 cut(s) 359, 803, 2090
BsrI ACTGG 7 cut(s) 296, 980, 1099, 1513, 1992, 2199, 2275
BssAI RCCGGY 3 cut(s) 359, 803, 2090
BssMI GATC 8 cut(s) 299, 333, 520, 766, 984, 1066, 1411, 1696
BssNI GRCGYC 8 cut(s) 111, 192, 357, 444, 543, 599, 672, 1179
BssT1I CCWWGG 6 cut(s) 558, 873, 1345, 1777, 1848, 2264
Bst2UI CCWGG 5 cut(s) 423, 606, 1002, 1244, 1937
Bst4CI ACNGT 6 cut(s) 658, 1038, 1328, 1591, 1880, 2120
Bst6I CTCTTC 1 cut(s) 2232
BstACI GRCGYC 8 cut(s) 111, 192, 357, 444, 543, 599, 672, 1179
BstBAI YACGTR 3 cut(s) 217, 946, 952
BstC8I GCNNGC 9 cut(s) 205, 233, 361, 365, 497, 824, 960, 1007, 1315
BstDEI CTNAG 4 cut(s) 330, 1959, 2071, 2184
BstDSI CCRYGG 2 cut(s) 1074, 2264
BstEII GGTNACC 1 cut(s) 1840
BstENI CCTNNNNNAGG 2 cut(s) 320, 1010
BstFNI CGCG 4 cut(s) 312, 408, 669, 1680
BstHHI GCGC 3 cut(s) 44, 410, 565
BstKTI GATC 8 cut(s) 302, 336, 523, 769, 987, 1069, 1414, 1699
BstMAI GTCTC 3 cut(s) 320, 1793, 2124
BstMBI GATC 8 cut(s) 299, 333, 520, 766, 984, 1066, 1411, 1696
BstMCI CGRYCG 2 cut(s) 310, 1372
BstMWI GCNNNNNNNGC 7 cut(s) 200, 405, 727, 823, 1271, 1541, 1553
BstNI CCWGG 5 cut(s) 423, 606, 1002, 1244, 1937
BstNSI RCATGY 2 cut(s) 1510, 2083
BstPI GGTNACC 1 cut(s) 1840
BstSCI CCNGG 8 cut(s) 169, 421, 604, 715, 1000, 1042, 1242, 1935
BstSLI GKGCMC 6 cut(s) 225, 635, 663, 713, 1397, 2159
BstUI CGCG 4 cut(s) 312, 408, 669, 1680
BstV1I GCAGC 4 cut(s) 219, 813, 1531, 1640
BstV2I GAAGAC 1 cut(s) 602
BstX2I RGATCY 5 cut(s) 299, 520, 984, 1066, 1696
BstYI RGATCY 5 cut(s) 299, 520, 984, 1066, 1696
BstZI CGGCCG 2 cut(s) 307, 1369
BtgI CCRYGG 2 cut(s) 1074, 2264
BtrI CACGTC 1 cut(s) 97
BveI ACCTGC 1 cut(s) 460
Cac8I GCNNGC 9 cut(s) 205, 233, 361, 365, 497, 824, 960, 1007, 1315
CciI TCATGA 1 cut(s) 252
CfoI GCGC 3 cut(s) 44, 410, 565
Cfr10I RCCGGY 3 cut(s) 359, 803, 2090
Cfr13I GGNCC 8 cut(s) 4, 282, 366, 790, 877, 990, 1108, 1568
CseI GACGC 7 cut(s) 200, 365, 551, 607, 661, 1168, 2169
Csp6I GTAC 7 cut(s) 949, 973, 1660, 1890, 1927, 2274, 2299
CviQI GTAC 7 cut(s) 949, 973, 1660, 1890, 1927, 2274, 2299
DdeI CTNAG 4 cut(s) 330, 1959, 2071, 2184
DpnI GATC 8 cut(s) 301, 335, 522, 768, 986, 1068, 1413, 1698
DpnII GATC 8 cut(s) 299, 333, 520, 766, 984, 1066, 1411, 1696
DraIII CACNNNGTG 1 cut(s) 220
EaeI YGGCCR 7 cut(s) 91, 229, 307, 1071, 1231, 1369, 1711
EagI CGGCCG 2 cut(s) 307, 1369
Eam1104I CTCTTC 1 cut(s) 2232
EarI CTCTTC 1 cut(s) 2232
EclXI CGGCCG 2 cut(s) 307, 1369
Eco130I CCWWGG 6 cut(s) 558, 873, 1345, 1777, 1848, 2264
Eco24I GRGCYC 3 cut(s) 1146, 1796, 1995
Eco47I GGWCC 4 cut(s) 282, 877, 1108, 1568
Eco52I CGGCCG 2 cut(s) 307, 1369
Eco57I CTGAAG 3 cut(s) 257, 1178, 1260
Eco72I CACGTG 1 cut(s) 217
Eco88I CYCGRG 1 cut(s) 317
Eco91I GGTNACC 1 cut(s) 1840
EcoNI CCTNNNNNAGG 2 cut(s) 320, 1010
EcoO109I RGGNCCY 1 cut(s) 366
EcoO65I GGTNACC 1 cut(s) 1840
EcoRII CCWGG 5 cut(s) 421, 604, 1000, 1242, 1935
EcoT14I CCWWGG 6 cut(s) 558, 873, 1345, 1777, 1848, 2264
EcoT38I GRGCYC 3 cut(s) 1146, 1796, 1995
ErhI CCWWGG 6 cut(s) 558, 873, 1345, 1777, 1848, 2264
FalI AAGNNNNNCTT 4 cut(s) 551, 583, 1589, 1621
FaqI GGGAC 3 cut(s) 98, 1094, 1517
FauI CCCGC 2 cut(s) 393, 1685
FblI GTMKAC 3 cut(s) 33, 615, 1188
FriOI GRGCYC 3 cut(s) 1146, 1796, 1995
FspBI CTAG 7 cut(s) 1314, 1551, 1625, 1803, 1949, 2040, 2308
GlaI GCGC 3 cut(s) 43, 409, 564
GsuI CTGGAG 2 cut(s) 1810, 2009
HgaI GACGC 7 cut(s) 200, 365, 551, 607, 661, 1168, 2169
HhaI GCGC 3 cut(s) 44, 410, 565
Hin1I GRCGYC 8 cut(s) 111, 192, 357, 444, 543, 599, 672, 1179
Hin6I GCGC 3 cut(s) 42, 408, 563
HinP1I GCGC 3 cut(s) 42, 408, 563
HincII GTYRAC 7 cut(s) 34, 475, 616, 940, 1090, 1126, 1189
HindII GTYRAC 7 cut(s) 34, 475, 616, 940, 1090, 1126, 1189
HindIII AAGCTT 1 cut(s) 692
HinfI GANTC 4 cut(s) 328, 486, 761, 2132
HpaI GTTAAC 1 cut(s) 1126
Hpy188I TCNGA 8 cut(s) 109, 299, 333, 355, 430, 766, 1763, 2185
Hpy188III TCNNGA 3 cut(s) 253, 931, 1168
HpyCH4III ACNGT 6 cut(s) 658, 1038, 1328, 1591, 1880, 2120
HpyCH4IV ACGT 9 cut(s) 96, 111, 216, 372, 444, 575, 945, 951, 1191
HpyCH4V TGCA 7 cut(s) 633, 661, 822, 1395, 1435, 1912, 2202
HpyF10VI GCNNNNNNNGC 7 cut(s) 200, 405, 727, 823, 1271, 1541, 1553
HpyF3I CTNAG 4 cut(s) 330, 1959, 2071, 2184
HpySE526I ACGT 9 cut(s) 96, 111, 216, 372, 444, 575, 945, 951, 1191
Hsp92I GRCGYC 8 cut(s) 111, 192, 357, 444, 543, 599, 672, 1179
HspAI GCGC 3 cut(s) 42, 408, 563
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
KspAI GTTAAC 1 cut(s) 1126
Kzo9I GATC 8 cut(s) 299, 333, 520, 766, 984, 1066, 1411, 1696
LmnI GCTCC 8 cut(s) 393, 431, 875, 1149, 1321, 1763, 1791, 1990
Lsp1109I GCAGC 4 cut(s) 219, 813, 1531, 1640
LweI GCATC 4 cut(s) 267, 618, 631, 1252
MaeI CTAG 7 cut(s) 1314, 1551, 1625, 1803, 1949, 2040, 2308
MaeII ACGT 9 cut(s) 96, 111, 216, 372, 444, 575, 945, 951, 1191
MaeIII GTNAC 9 cut(s) 1038, 1328, 1379, 1442, 1481, 1583, 1840, 1983, 2026
MalI GATC 8 cut(s) 301, 335, 522, 768, 986, 1068, 1413, 1698
MbiI CCGCTC 2 cut(s) 388, 459
MboI GATC 8 cut(s) 299, 333, 520, 766, 984, 1066, 1411, 1696
MboII GAAGA 7 cut(s) 253, 497, 530, 607, 1063, 1076, 2249
MflI RGATCY 5 cut(s) 299, 520, 984, 1066, 1696
MluCI AATT 7 cut(s) 10, 1728, 1826, 1893, 2010, 2054, 2142
MlyI GAGTC 3 cut(s) 322, 755, 2126
MmeI TCCRAC 7 cut(s) 99, 132, 277, 327, 378, 567, 1185
MroNI GCCGGC 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 1289
MseI TTAA 2 cut(s) 1125, 1770
MslI CAYNNNNRTG 2 cut(s) 533, 1527
MspA1I CMGCKG 1 cut(s) 235
MspR9I CCNGG 8 cut(s) 171, 423, 606, 717, 1002, 1044, 1244, 1937
MvaI CCWGG 5 cut(s) 423, 606, 1002, 1244, 1937
MvnI CGCG 4 cut(s) 312, 408, 669, 1680
MwoI GCNNNNNNNGC 7 cut(s) 200, 405, 727, 823, 1271, 1541, 1553
NaeI GCCGGC 1 cut(s) 361
NciI CCSGG 3 cut(s) 171, 717, 1044
NcoI CCATGG 1 cut(s) 2264
NdeII GATC 8 cut(s) 299, 333, 520, 766, 984, 1066, 1411, 1696
NgoMIV GCCGGC 1 cut(s) 359
NheI GCTAGC 1 cut(s) 1313
NmeAIII GCCGAG 3 cut(s) 223, 914, 1347
NmuCI GTSAC 7 cut(s) 1038, 1379, 1442, 1583, 1840, 1983, 2026
NspI RCATGY 2 cut(s) 1510, 2083
OliI CACNNNNGTG 1 cut(s) 533
PaeR7I CTCGAG 1 cut(s) 317
PagI TCATGA 1 cut(s) 252
PciI ACATGT 1 cut(s) 1506
PcsI WCGNNNNNNNCGW 4 cut(s) 195, 222, 342, 351
PdiI GCCGGC 1 cut(s) 361
PdmI GAANNNNTTC 1 cut(s) 1289
PfeI GAWTC 1 cut(s) 486
PflFI GACNNNGTC 3 cut(s) 545, 863, 1178
PflMI CCANNNNNTGG 3 cut(s) 291, 1204, 2270
PleI GAGTC 3 cut(s) 322, 755, 2126
PmaCI CACGTG 1 cut(s) 217
PmlI CACGTG 1 cut(s) 217
PpsI GAGTC 3 cut(s) 322, 755, 2126
Ppu21I YACGTR 3 cut(s) 217, 946, 952
PscI ACATGT 1 cut(s) 1506
Psp1406I AACGTT 1 cut(s) 575
Psp6I CCWGG 5 cut(s) 421, 604, 1000, 1242, 1935
PspCI CACGTG 1 cut(s) 217
PspEI GGTNACC 1 cut(s) 1840
PspGI CCWGG 5 cut(s) 421, 604, 1000, 1242, 1935
PspPI GGNCC 8 cut(s) 4, 282, 366, 790, 877, 990, 1108, 1568
PspXI VCTCGAGB 1 cut(s) 317
PsuI RGATCY 5 cut(s) 299, 520, 984, 1066, 1696
PsyI GACNNNGTC 3 cut(s) 545, 863, 1178
PvuII CAGCTG 1 cut(s) 235
RsaI GTAC 7 cut(s) 950, 974, 1661, 1891, 1928, 2275, 2300
RsaNI GTAC 7 cut(s) 949, 973, 1660, 1890, 1927, 2274, 2299
RseI CAYNNNNRTG 2 cut(s) 533, 1527
SalI GTCGAC 3 cut(s) 32, 614, 1187
SaqAI TTAA 2 cut(s) 1125, 1770
Sau3AI GATC 8 cut(s) 299, 333, 520, 766, 984, 1066, 1411, 1696
Sau96I GGNCC 8 cut(s) 4, 282, 366, 790, 877, 990, 1108, 1568
ScaI AGTACT 1 cut(s) 1661
SchI GAGTC 3 cut(s) 322, 755, 2126
ScrFI CCNGG 8 cut(s) 171, 423, 606, 717, 1002, 1044, 1244, 1937
SfaNI GCATC 4 cut(s) 267, 618, 631, 1252
Sfr274I CTCGAG 1 cut(s) 317
SgrAI CRCCGGYG 2 cut(s) 803, 2090
SinI GGWCC 4 cut(s) 282, 877, 1108, 1568
SlaI CTCGAG 1 cut(s) 317
SmiMI CAYNNNNRTG 2 cut(s) 533, 1527
SmlI CTYRAG 2 cut(s) 317, 830
SmoI CTYRAG 2 cut(s) 317, 830
Sse9I AATT 7 cut(s) 10, 1728, 1826, 1893, 2010, 2054, 2142
SspMI CTAG 7 cut(s) 1314, 1551, 1625, 1803, 1949, 2040, 2308
StyD4I CCNGG 8 cut(s) 169, 421, 604, 715, 1000, 1042, 1242, 1935
StyI CCWWGG 6 cut(s) 558, 873, 1345, 1777, 1848, 2264
TaaI ACNGT 6 cut(s) 658, 1038, 1328, 1591, 1880, 2120
TaiI ACGT 9 cut(s) 99, 114, 219, 375, 447, 578, 948, 954, 1194
TaqI TCGA 8 cut(s) 33, 189, 318, 345, 441, 615, 1188, 1293
TasI AATT 7 cut(s) 10, 1728, 1826, 1893, 2010, 2054, 2142
TatI WGTACW 4 cut(s) 1659, 1889, 1926, 2298
TauI GCSGC 8 cut(s) 197, 213, 312, 402, 672, 803, 1535, 1716
TfiI GAWTC 1 cut(s) 486
Tru1I TTAA 2 cut(s) 1125, 1770
Tru9I TTAA 2 cut(s) 1125, 1770
TseFI GTSAC 7 cut(s) 1038, 1379, 1442, 1583, 1840, 1983, 2026
TseI GCWGC 4 cut(s) 207, 826, 1544, 1653
Tsp45I GTSAC 7 cut(s) 1038, 1379, 1442, 1583, 1840, 1983, 2026
TspDTI ATGAA 8 cut(s) 241, 844, 1152, 1327, 1442, 1477, 2246, 2250
TspGWI ACGGA 1 cut(s) 1153
Tth111I GACNNNGTC 3 cut(s) 545, 863, 1178
Van91I CCANNNNNTGG 3 cut(s) 291, 1204, 2270
VneI GTGCAC 3 cut(s) 631, 659, 1393
VpaK11BI GGWCC 4 cut(s) 282, 877, 1108, 1568
XagI CCTNNNNNAGG 2 cut(s) 320, 1010
XapI RAATTY 3 cut(s) 10, 1728, 1826
XceI RCATGY 2 cut(s) 1510, 2083
XcmI CCANNNNNNNNNTGG 2 cut(s) 565, 784
XhoI CTCGAG 1 cut(s) 317
XmiI GTMKAC 3 cut(s) 33, 615, 1188
XmnI GAANNNNTTC 1 cut(s) 1289
XspI CTAG 7 cut(s) 1314, 1551, 1625, 1803, 1949, 2040, 2308
ZraI GACGTC 2 cut(s) 112, 445
ZrmI AGTACT 1 cut(s) 1661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.