Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005392

Physical Location & Seq

Genomic Coordinates Reverse (-)

2164925 .. 2169057

Gene Structure 4133 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1030100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2301 bp

ATGGCTCCAAACTTGAGCAAAGTGATATCCGACGTCAAGGGCTCTATGCCGACGTCTTGTTCGTTTAAGCTTGAAAAGTCCAATTTCATGGCAAATGGCCACGTCATCCTCTCGGACGTCCCCAAAAATATCACTCTGACTCCATCCCTCTCCACAGCCGATGGTTGCTTCGTGGGATTCAATGCCATCGAGGCAAGCAGCCGCCATGTTGTTCCCGTCGGCCAACTGAAGGACATCCGATTCATGAGCATTTTCCGGTTCAAGGTCTGGTGGACCACCCACTGGGTCGGATCCAAAGGCCGAGACCTCGAGAACGAGACTCAGATCGTTGTTCTTGAGAGCTCGGACGCCGGGAGGCCCTTCGTCGTGCTCCTCCCCATCCTAGAAGGATCCTTCCGCGCTTCCGTCCAGCCTGGAAACGAGGACTTTCTCGACATATGCGTCGAGAGCGGGTCCACCAAGGAAACCGCCAGCACCTTCCAGAGCGTTCTTTATCTACACGCCGGCGACGATCCGTTTACTCTTGTCAAAGACGCCGTCAAAGCCGCAAGAGATCATTTGGGAACTTTCAAGCTTCTCGAGGAGAAGACCCCACCTGGCATCGTGGACAAATTTGGCTGGTGCACTTGGGACGCTTTCTATCTTACCGTGCACCCTCACGGCGTCATCGACGGCGTGAGGAAGCTCGTGGAAGGCGGGTGCCCTCCCGGCCTCGTCTTGCTCGACGACGGGTGGCAGTCCATCGCCCACGACTCTGACCCCATCACCCAAGAAGGCATTAATCAGACGGTCGCCGGTGAGCAAATGCCCTGCAGGCTCCTGAAGTTTCAAGAAAATTACAAGTTCAGGGACTATGTGAGCCCCACCAGGGGTGACTCTAGTAAAGGCATGGGTGCCTTTATCAAAGACCTCAAGGAGGAGTTCAAGAGCGTTGATTATGTGTACGTGTGGCACGCGCTTTGCGGGTACTGGGGTGGGGTCCGCCCCAATGTCCCCGGCATGCCTGAGGCAGTGGTGGTGGAGCCCAAATTATCTCCAGGTTTGCAGAAGACGATGGAAGATCTGGCCGTTGATAAGATCGTGGCCACCGGAATTGGGCTGGTCCCACCAGAAATAGTTGATCAGATGTACGAGGGGCTCCACTCGCACTTGAAGTCTGTTGGGATCGACGGTGTAAAAGTCGACGTTATCCATTTGTTGGAGATGCTTTGTGAGAACTATGGTGGGCGTGTGAAGCTAGCAAAAGCATACTTCGATGCCCTAACATCCTCAGTGAGGAAGCACTTCAATGGCAATGGCGTCATTGCAAGCATGGAGCACTGCAACGACTTCATGTTCCTTGGCACAGAAGCAATCACTCTTGGCCGTGTTGGTGATGACTTCTGGTGCACGGATCCATCTGGAGACCCAAACGGTACGTTTTGGCTCCAAGGGTGCCACATGGTGCACTGCGCCTACAATAGCTTGTGGATGGGGAATTTCATACACCCCGACTGGGACATGTTCCAGTCGACCCACCCTTGCGCCGCCTTCCATGCCGCCTCTAGGGCCATCTCCGGCGGTCCTATTTATGTGAGCGACGCCGTTGGAAAGCACAACTTTGACTTGCTCAAGACCCTGGTGCTGCCCGATGGCTCTATTCTCCGGTGCGAGTACTATGCCCTCCCTACTCGTGATTGTCTCTTTGAGGACCCTCTTCATGATGGTAACACCATGCTCAAAATATGGAACCTCAACAAGTACACCGGAGTTCTTGGGGCATTCAACTGCCAAGGAGGAGGGTGGAGCCGGGAGAACCGTCGCAACCAATGTTTTGCTGAATTCTCCCACCGGCTGACCGCCAAGTTCAACCCCAAAGACATCCAGTGGAAGAGCGGCAAGAATCCGATCTCCGTCGACGGAGTCGAGGCCTTTGCCATGTACTACCACCAGTCCAAGAAGCTAGTCCTATCCAAGCCAAACGAGGATGCAGAACTCTCACTTGACCCCTTCAACTTTGAGCTCATTTTTGTGTCCCCGGTGACTGTTTTATCCGCTAGCAAAAATGTCCAGTTTGCCCCCATTGGCTTAGTCAACATGCTCAACACCGGCGGTGCCATCCAATCATTCAACGAGGAGGGCAATTCCGTCCAAATCGGGGTTAGAGGCGCTGGAGAGCTGAGAGTATTTGCTTCTGAGAAGCCAACAAGTTGCAGAATTGGTGGAAACGACGTCGCCTTCGAATATGAGCAACAAATGGTCGTAATCCATGTGCCATGGCCTGCTTCTTCCAACTTGTCTAATGTGAAGTACATATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006950 GO:0006979 GO:0008150 GO:0008378 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010035 GO:0016740 GO:0016757 GO:0016758 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0047274 GO:0050896 GO:0097305 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00052 map00052

Pfam Domains

Protein Families

Raffinose_syn

Protein Analysis

767

Amino Acids

84.57

Weight (kDa)

5.69

Isoelectric Point (pI)

30.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Raffinose_syn	PF05691	23 - 748	0.0	Raffinose synthase or seed imbibition protein Sip1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005392)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40390
fragaria_vesca	FvH4_5g05860 FvH4_6g51380
malus_domestica	MD06G1169800.v1.1 MD14G1175300.v1.1 MD17G1030100.v1.1
prunus_persica	Prupe.3G289900_v2.0.a1
pyrus_communis	pycom06g15120 pycom14g14630 pycom17g02750
rosa_chinensis	RchiOBHm_Chr2g0173061 RchiOBHm_Chr7g0192961
rosa_laevigata	RLG00000004308 RLG00000022154
rosa_multiflora	Rmu_sc0002040.1_g000062 Rmu_sc0011236.1_g000005
rosa_roxburghii	Rroxscaffold_2G00079280 Rroxscaffold_3G00262500
rosa_rugosa	Rorug02G0564900 Rorug07G0006500
rosa_samantha	Rh2AG644700 Rh2BG655500 Rh2CG620600 Rh2DG668800 Rh7AG131800 Rh7BG132600 Rh7DG133700
rosa_wichuraiana	Rw2G052950 Rw7G011240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	440
AatII	GACGTC	4 cut(s)	36, 56, 120, 2214
AccB1I	GGYRCC	4 cut(s)	699, 893, 1434, 2093
AccB7I	CCANNNNNTGG	2 cut(s)	282, 1198
AccBSI	CCGCTC	2 cut(s)	450, 1875
AccI	GTMKAC	3 cut(s)	1182, 1511, 1896
AccII	CGCG	2 cut(s)	399, 956
AclWI	GGATC	8 cut(s)	285, 298, 384, 397, 506, 1172, 1388, 1401
AcoI	YGGCCR	5 cut(s)	97, 220, 1065, 1083, 1363
AcsI	RAATTY	3 cut(s)	611, 1477, 1820
AcuI	CTGAAG	2 cut(s)	248, 842
AcyI	GRCGYC	9 cut(s)	33, 53, 117, 348, 534, 663, 1299, 1581, 2211
AdeI	CACNNNGTG	1 cut(s)	1444
AfaI	GTAC	8 cut(s)	944, 968, 1130, 1417, 1655, 1742, 1922, 2291
AflIII	ACRYGT	2 cut(s)	945, 1500
AjiI	CACGTC	1 cut(s)	103
AjnI	CCWGG	5 cut(s)	412, 595, 866, 1036, 1617
AluBI	AGCT	9 cut(s)	70, 342, 574, 685, 1237, 1464, 1942, 2002, 2158
AluI	AGCT	9 cut(s)	70, 342, 574, 685, 1237, 1464, 1942, 2002, 2158
Alw21I	GWGCWC	8 cut(s)	344, 372, 626, 654, 1320, 1391, 1449, 2004
Alw26I	GTCTC	4 cut(s)	297, 311, 1398, 1685
Alw44I	GTGCAC	4 cut(s)	622, 650, 1387, 1445
AlwI	GGATC	8 cut(s)	285, 298, 384, 397, 506, 1172, 1388, 1401
Ama87I	CYCGRG	2 cut(s)	308, 578
ApaLI	GTGCAC	4 cut(s)	622, 650, 1387, 1445
ApeKI	GCWGC	2 cut(s)	198, 1624
ApoI	RAATTY	3 cut(s)	611, 1477, 1820
ArsI	GACNNNNNNTTYG	6 cut(s)	43, 75, 522, 554, 2201, 2233
AseI	ATTAAT	1 cut(s)	780
Asp700I	GAANNNNTTC	1 cut(s)	1283
AspLEI	GCGC	5 cut(s)	401, 958, 1454, 1526, 2150
AspS9I	GGNCC	8 cut(s)	273, 357, 453, 979, 1102, 1548, 1562, 1690
AsuC2I	CCSGG	5 cut(s)	352, 708, 996, 1790, 2018
AsuHPI	GGTGA	5 cut(s)	757, 809, 884, 1385, 2032
AsuII	TTCGAA	1 cut(s)	2220
AsuNHI	GCTAGC	2 cut(s)	1237, 2036
AvaI	CYCGRG	2 cut(s)	308, 578
AvaII	GGWCC	6 cut(s)	273, 453, 979, 1102, 1562, 1690
AxyI	CCTNAGG	1 cut(s)	1005
BaeGI	GKGCMC	5 cut(s)	626, 654, 704, 1391, 1449
BaeI	ACNNNNGTAYC	2 cut(s)	958, 991
BalI	TGGCCA	2 cut(s)	99, 1085
BamHI	GGATCC	3 cut(s)	290, 389, 1393
BanI	GGYRCC	4 cut(s)	699, 893, 1434, 2093
BanII	GRGCYC	6 cut(s)	44, 344, 863, 1026, 1140, 2004
BauI	CACGAG	2 cut(s)	686, 1671
BbsI	GAAGAC	2 cut(s)	593, 1055
Bbv12I	GWGCWC	8 cut(s)	344, 372, 626, 654, 1320, 1391, 1449, 2004
BbvI	GCAGC	2 cut(s)	210, 1611
BceAI	ACGGC	6 cut(s)	521, 676, 688, 1052, 1350, 1568
BcgI	CGANNNNNNTGC	4 cut(s)	1640, 1674, 1895, 1929
BciT130I	CCWGG	5 cut(s)	414, 597, 868, 1038, 1619
BclI	TGATCA	1 cut(s)	1120
BcnI	CCSGG	5 cut(s)	352, 708, 996, 1790, 2018
BcoDI	GTCTC	4 cut(s)	297, 311, 1398, 1685
BfaI	CTAG	6 cut(s)	383, 879, 1238, 1545, 1943, 2037
BfmI	CTRYAG	1 cut(s)	811
BfoI	RGCGCY	1 cut(s)	2151
BglI	GCCNNNNNGGC	4 cut(s)	191, 708, 814, 1547
BglII	AGATCT	1 cut(s)	1060
BisI	GCNGC	7 cut(s)	199, 202, 546, 1527, 1539, 1625, 1876
BlsI	GCNGC	7 cut(s)	200, 203, 547, 1528, 1540, 1626, 1877
BmcAI	AGTACT	1 cut(s)	1655
Bme18I	GGWCC	6 cut(s)	273, 453, 979, 1102, 1562, 1690
BmeT110I	CYCGRG	2 cut(s)	308, 578
BmgBI	CACGTC	1 cut(s)	103
BmgT120I	GGNCC	8 cut(s)	273, 357, 453, 979, 1102, 1548, 1562, 1690
BmrI	ACTGGG	3 cut(s)	292, 979, 1504
BmsI	GCATC	4 cut(s)	609, 1194, 1246, 1957
BmtI	GCTAGC	2 cut(s)	1241, 2040
BmuI	ACTGGG	3 cut(s)	292, 979, 1504
BpiI	GAAGAC	2 cut(s)	593, 1055
BpmI	CTGGAG	3 cut(s)	1020, 1422, 2172
Bpu14I	TTCGAA	1 cut(s)	2220
BpuEI	CTTGAG	4 cut(s)	34, 356, 896, 1595
BpuMI	CCSGG	5 cut(s)	352, 708, 996, 1790, 2018
BsaAI	YACGTR	1 cut(s)	946
BsaHI	GRCGYC	9 cut(s)	33, 53, 117, 348, 534, 663, 1299, 1581, 2211
BsaI	GGTCTC	2 cut(s)	297, 1398
BsaJI	CCNNGG	9 cut(s)	459, 867, 994, 1339, 1429, 1617, 1771, 2016, 2255
BsaWI	WCCGGW	4 cut(s)	255, 1088, 1644, 1745
BsaXI	ACNNNNNCTCC	2 cut(s)	124, 154
Bse118I	RCCGGY	4 cut(s)	503, 794, 1830, 2087
Bse1I	ACTGG	7 cut(s)	287, 974, 1499, 1507, 1864, 1930, 2050
Bse21I	CCTNAGG	1 cut(s)	1005
Bse3DI	GCAATG	2 cut(s)	1300, 1302
BseBI	CCWGG	5 cut(s)	414, 597, 868, 1038, 1619
BseDI	CCNNGG	9 cut(s)	459, 867, 994, 1339, 1429, 1617, 1771, 2016, 2255
BseGI	GGATG	9 cut(s)	105, 143, 234, 378, 1265, 1476, 1860, 1972, 2097
BseMI	GCAATG	2 cut(s)	1300, 1302
BseMII	CTCAG	5 cut(s)	335, 996, 1284, 2150, 2166
BseNI	ACTGG	7 cut(s)	287, 974, 1499, 1507, 1864, 1930, 2050
BseRI	GAGGAG	5 cut(s)	362, 596, 932, 1791, 2129
BseSI	GKGCMC	5 cut(s)	626, 654, 704, 1391, 1449
BseXI	GCAGC	2 cut(s)	210, 1611
Bsh1236I	CGCG	2 cut(s)	399, 956
Bsh1285I	CGRYCG	1 cut(s)	792
BshNI	GGYRCC	4 cut(s)	699, 893, 1434, 2093
BsiEI	CGRYCG	1 cut(s)	792
BsiHKAI	GWGCWC	8 cut(s)	344, 372, 626, 654, 1320, 1391, 1449, 2004
BsiHKCI	CYCGRG	2 cut(s)	308, 578
BslFI	GGGAC	7 cut(s)	104, 644, 863, 977, 1088, 1511, 1999
BsmAI	GTCTC	4 cut(s)	297, 311, 1398, 1685
BsmFI	GGGAC	7 cut(s)	104, 644, 863, 977, 1088, 1511, 1999
BsmI	GAATGC	1 cut(s)	1760
Bso31I	GGTCTC	2 cut(s)	297, 1398
BsoBI	CYCGRG	2 cut(s)	308, 578
Bsp119I	TTCGAA	1 cut(s)	2220
Bsp19I	CCATGG	1 cut(s)	2255
BspCNI	CTCAG	5 cut(s)	334, 997, 1283, 2151, 2167
BspFNI	CGCG	2 cut(s)	399, 956
BspHI	TCATGA	2 cut(s)	243, 1699
BspMAI	CTGCAG	1 cut(s)	815
BspOI	GCTAGC	2 cut(s)	1241, 2040
BspPI	GGATC	8 cut(s)	285, 298, 384, 397, 506, 1172, 1388, 1401
BspQI	GCTCTTC	1 cut(s)	1865
BspT104I	TTCGAA	1 cut(s)	2220
BspT107I	GGYRCC	4 cut(s)	699, 893, 1434, 2093
BspTNI	GGTCTC	2 cut(s)	297, 1398
BsrBI	CCGCTC	2 cut(s)	450, 1875
BsrDI	GCAATG	2 cut(s)	1300, 1302
BsrFI	RCCGGY	4 cut(s)	503, 794, 1830, 2087
BsrI	ACTGG	7 cut(s)	287, 974, 1499, 1507, 1864, 1930, 2050
BssAI	RCCGGY	4 cut(s)	503, 794, 1830, 2087
BssECI	CCNNGG	9 cut(s)	459, 867, 994, 1339, 1429, 1617, 1771, 2016, 2255
BssNI	GRCGYC	9 cut(s)	33, 53, 117, 348, 534, 663, 1299, 1581, 2211
BssSI	CACGAG	2 cut(s)	686, 1671
BssT1I	CCWWGG	5 cut(s)	459, 1339, 1429, 1771, 2255
Bst2BI	CACGAG	2 cut(s)	686, 1671
Bst2UI	CCWGG	5 cut(s)	414, 597, 868, 1038, 1619
Bst4CI	ACNGT	6 cut(s)	649, 790, 1172, 1415, 1799, 2026
Bst6I	CTCTTC	2 cut(s)	1701, 1865
BstACI	GRCGYC	9 cut(s)	33, 53, 117, 348, 534, 663, 1299, 1581, 2211
BstBAI	YACGTR	1 cut(s)	946
BstBI	TTCGAA	1 cut(s)	2220
BstDEI	CTNAG	6 cut(s)	321, 1005, 1270, 2068, 2159, 2175
BstDSI	CCRYGG	1 cut(s)	2255
BstENI	CCTNNNNNAGG	2 cut(s)	914, 1273
BstF5I	GGATG	9 cut(s)	105, 143, 234, 378, 1265, 1476, 1860, 1972, 2097
BstFNI	CGCG	2 cut(s)	399, 956
BstH2I	RGCGCY	1 cut(s)	2151
BstHHI	GCGC	5 cut(s)	401, 958, 1454, 1526, 2150
BstMAI	GTCTC	4 cut(s)	297, 311, 1398, 1685
BstMCI	CGRYCG	1 cut(s)	792
BstMWI	GCNNNNNNNGC	9 cut(s)	191, 447, 542, 708, 814, 1144, 1234, 1535, 1547
BstNI	CCWGG	5 cut(s)	414, 597, 868, 1038, 1619
BstNSI	RCATGY	3 cut(s)	1003, 1504, 2080
BstSFI	CTRYAG	1 cut(s)	811
BstSLI	GKGCMC	5 cut(s)	626, 654, 704, 1391, 1449
BstUI	CGCG	2 cut(s)	399, 956
BstV1I	GCAGC	2 cut(s)	210, 1611
BstV2I	GAAGAC	2 cut(s)	593, 1055
BstX2I	RGATCY	4 cut(s)	290, 389, 1060, 1393
BstXI	CCANNNNNNTGG	1 cut(s)	88
BstYI	RGATCY	4 cut(s)	290, 389, 1060, 1393
Bsu36I	CCTNAGG	1 cut(s)	1005
BtgI	CCRYGG	1 cut(s)	2255
BtgZI	GCGATG	1 cut(s)	727
BtrI	CACGTC	1 cut(s)	103
BtsCI	GGATG	9 cut(s)	105, 143, 234, 378, 1265, 1476, 1860, 1972, 2097
BtsI	GCAGTG	3 cut(s)	1017, 1318, 1447
BtsIMutI	CAGTG	6 cut(s)	280, 1017, 1278, 1318, 1447, 1871
CciI	TCATGA	2 cut(s)	243, 1699
CfoI	GCGC	5 cut(s)	401, 958, 1454, 1526, 2150
Cfr10I	RCCGGY	4 cut(s)	503, 794, 1830, 2087
Cfr13I	GGNCC	8 cut(s)	273, 357, 453, 979, 1102, 1548, 1562, 1690
CseI	GACGC	7 cut(s)	356, 430, 542, 641, 652, 1288, 1589
Csp6I	GTAC	8 cut(s)	943, 967, 1129, 1416, 1654, 1741, 1921, 2290
CspCI	CAANNNNNGTGG	2 cut(s)	1075, 1110
CviQI	GTAC	8 cut(s)	943, 967, 1129, 1416, 1654, 1741, 1921, 2290
DdeI	CTNAG	6 cut(s)	321, 1005, 1270, 2068, 2159, 2175
DraIII	CACNNNGTG	1 cut(s)	1444
DrdI	GACNNNNNNGTC	1 cut(s)	440
DseDI	GACNNNNNNGTC	1 cut(s)	440
EaeI	YGGCCR	5 cut(s)	97, 220, 1065, 1083, 1363
Eam1104I	CTCTTC	2 cut(s)	1701, 1865
EarI	CTCTTC	2 cut(s)	1701, 1865
EciI	GGCGGA	1 cut(s)	971
Ecl136II	GAGCTC	2 cut(s)	342, 2002
Eco130I	CCWWGG	5 cut(s)	459, 1339, 1429, 1771, 2255
Eco147I	AGGCCT	1 cut(s)	1910
Eco24I	GRGCYC	6 cut(s)	44, 344, 863, 1026, 1140, 2004
Eco31I	GGTCTC	2 cut(s)	297, 1398
Eco32I	GATATC	1 cut(s)	27
Eco47I	GGWCC	6 cut(s)	273, 453, 979, 1102, 1562, 1690
Eco53kI	GAGCTC	2 cut(s)	342, 2002
Eco57I	CTGAAG	2 cut(s)	248, 842
Eco81I	CCTNAGG	1 cut(s)	1005
Eco88I	CYCGRG	2 cut(s)	308, 578
EcoICRI	GAGCTC	2 cut(s)	342, 2002
EcoNI	CCTNNNNNAGG	2 cut(s)	914, 1273
EcoO109I	RGGNCCY	2 cut(s)	357, 1690
EcoRI	GAATTC	1 cut(s)	1820
EcoRII	CCWGG	5 cut(s)	412, 595, 866, 1036, 1617
EcoRV	GATATC	1 cut(s)	27
EcoT14I	CCWWGG	5 cut(s)	459, 1339, 1429, 1771, 2255
EcoT38I	GRGCYC	6 cut(s)	44, 344, 863, 1026, 1140, 2004
ErhI	CCWWGG	5 cut(s)	459, 1339, 1429, 1771, 2255
FalI	AAGNNNNNCTT	2 cut(s)	1583, 1615
FaqI	GGGAC	7 cut(s)	104, 644, 863, 977, 1088, 1511, 1999
FauI	CCCGC	3 cut(s)	443, 689, 956
FauNDI	CATATG	1 cut(s)	437
FbaI	TGATCA	1 cut(s)	1120
FblI	GTMKAC	3 cut(s)	1182, 1511, 1896
Fnu4HI	GCNGC	7 cut(s)	199, 202, 546, 1527, 1539, 1625, 1876
FokI	GGATG	9 cut(s)	92, 130, 221, 365, 1252, 1483, 1847, 1979, 2084
FriOI	GRGCYC	6 cut(s)	44, 344, 863, 1026, 1140, 2004
Fsp4HI	GCNGC	7 cut(s)	199, 202, 546, 1527, 1539, 1625, 1876
FspBI	CTAG	6 cut(s)	383, 879, 1238, 1545, 1943, 2037
GlaI	GCGC	5 cut(s)	400, 957, 1453, 1525, 2149
GluI	GCNGC	7 cut(s)	199, 202, 546, 1527, 1539, 1625, 1876
GsuI	CTGGAG	3 cut(s)	1020, 1422, 2172
HaeII	RGCGCY	1 cut(s)	2151
HgaI	GACGC	7 cut(s)	356, 430, 542, 641, 652, 1288, 1589
HhaI	GCGC	5 cut(s)	401, 958, 1454, 1526, 2150
Hin1I	GRCGYC	9 cut(s)	33, 53, 117, 348, 534, 663, 1299, 1581, 2211
Hin6I	GCGC	5 cut(s)	399, 956, 1452, 1524, 2148
HinP1I	GCGC	5 cut(s)	399, 956, 1452, 1524, 2148
HincII	GTYRAC	4 cut(s)	1183, 1512, 1897, 2074
HindII	GTYRAC	4 cut(s)	1183, 1512, 1897, 2074
HindIII	AAGCTT	2 cut(s)	68, 572
HinfI	GANTC	8 cut(s)	139, 177, 240, 319, 752, 875, 1882, 1902
HphI	GGTGA	5 cut(s)	757, 809, 884, 1385, 2032
HpyCH4III	ACNGT	6 cut(s)	649, 790, 1172, 1415, 1799, 2026
HpyCH4IV	ACGT	8 cut(s)	33, 53, 102, 117, 945, 1185, 1418, 2211
HpyF10VI	GCNNNNNNNGC	9 cut(s)	191, 447, 542, 708, 814, 1144, 1234, 1535, 1547
HpyF3I	CTNAG	6 cut(s)	321, 1005, 1270, 2068, 2159, 2175
HpySE526I	ACGT	8 cut(s)	33, 53, 102, 117, 945, 1185, 1418, 2211
Hsp92I	GRCGYC	9 cut(s)	33, 53, 117, 348, 534, 663, 1299, 1581, 2211
HspAI	GCGC	5 cut(s)	399, 956, 1452, 1524, 2148
KroI	GCCGGC	1 cut(s)	503
KroNI	GCCGGC	1 cut(s)	505
Ksp22I	TGATCA	1 cut(s)	1120
LguI	GCTCTTC	1 cut(s)	1865
LmnI	GCTCC	8 cut(s)	10, 375, 822, 1021, 1143, 1315, 1431, 1785
Lsp1109I	GCAGC	2 cut(s)	210, 1611
LweI	GCATC	4 cut(s)	609, 1194, 1246, 1957
MaeI	CTAG	6 cut(s)	383, 879, 1238, 1545, 1943, 2037
MaeII	ACGT	8 cut(s)	33, 53, 102, 117, 945, 1185, 1418, 2211
MaeIII	GTNAC	3 cut(s)	872, 1706, 2020
MbiI	CCGCTC	2 cut(s)	450, 1875
MboII	GAAGA	6 cut(s)	598, 1060, 1070, 1688, 1882, 2259
MflI	RGATCY	4 cut(s)	290, 389, 1060, 1393
MlsI	TGGCCA	2 cut(s)	99, 1085
MluCI	AATT	9 cut(s)	82, 611, 835, 1028, 1092, 1477, 1820, 2122, 2196
MluNI	TGGCCA	2 cut(s)	99, 1085
MlyI	GAGTC	5 cut(s)	133, 313, 746, 869, 1911
MmeI	TCCRAC	5 cut(s)	54, 268, 1179, 1567, 2295
Mox20I	TGGCCA	2 cut(s)	99, 1085
MreI	CGCCGGCG	1 cut(s)	503
MroNI	GCCGGC	1 cut(s)	503
MroXI	GAANNNNTTC	1 cut(s)	1283
MscI	TGGCCA	2 cut(s)	99, 1085
MseI	TTAA	3 cut(s)	66, 780, 2299
MslI	CAYNNNNRTG	2 cut(s)	1287, 2009
Msp20I	TGGCCA	2 cut(s)	99, 1085
Mva1269I	GAATGC	1 cut(s)	1760
MvaI	CCWGG	5 cut(s)	414, 597, 868, 1038, 1619
MvnI	CGCG	2 cut(s)	399, 956
MwoI	GCNNNNNNNGC	9 cut(s)	191, 447, 542, 708, 814, 1144, 1234, 1535, 1547
NaeI	GCCGGC	1 cut(s)	505
NciI	CCSGG	5 cut(s)	352, 708, 996, 1790, 2018
NcoI	CCATGG	1 cut(s)	2255
NdeI	CATATG	1 cut(s)	437
NgoMIV	GCCGGC	1 cut(s)	503
NheI	GCTAGC	2 cut(s)	1237, 2036
NmeAIII	GCCGAG	1 cut(s)	326
NmuCI	GTSAC	2 cut(s)	872, 2020
NspI	RCATGY	3 cut(s)	1003, 1504, 2080
NspV	TTCGAA	1 cut(s)	2220
PaeI	GCATGC	1 cut(s)	1003
PaeR7I	CTCGAG	2 cut(s)	308, 578
PagI	TCATGA	2 cut(s)	243, 1699
PceI	AGGCCT	1 cut(s)	1910
PciI	ACATGT	1 cut(s)	1500
PciSI	GCTCTTC	1 cut(s)	1865
PcsI	WCGNNNNNNNCGW	7 cut(s)	59, 438, 507, 666, 720, 1902, 2217
PctI	GAATGC	1 cut(s)	1760
PdiI	GCCGGC	1 cut(s)	505
PdmI	GAANNNNTTC	1 cut(s)	1283
PfeI	GAWTC	3 cut(s)	177, 240, 1882
PflFI	GACNNNGTC	2 cut(s)	536, 1901
PflMI	CCANNNNNTGG	2 cut(s)	282, 1198
PkrI	GCNGC	7 cut(s)	200, 203, 547, 1528, 1540, 1626, 1877
PleI	GAGTC	5 cut(s)	133, 313, 746, 869, 1910
PpsI	GAGTC	5 cut(s)	133, 313, 746, 869, 1910
Ppu21I	YACGTR	1 cut(s)	946
PpuMI	RGGWCCY	1 cut(s)	1690
PscI	ACATGT	1 cut(s)	1500
PshBI	ATTAAT	1 cut(s)	780
Psp124BI	GAGCTC	2 cut(s)	344, 2004
Psp5II	RGGWCCY	1 cut(s)	1690
Psp6I	CCWGG	5 cut(s)	412, 595, 866, 1036, 1617
PspGI	CCWGG	5 cut(s)	412, 595, 866, 1036, 1617
PspPI	GGNCC	8 cut(s)	273, 357, 453, 979, 1102, 1548, 1562, 1690
PspPPI	RGGWCCY	1 cut(s)	1690
PstI	CTGCAG	1 cut(s)	815
PsuI	RGATCY	4 cut(s)	290, 389, 1060, 1393
PsyI	GACNNNGTC	2 cut(s)	536, 1901
RsaI	GTAC	8 cut(s)	944, 968, 1130, 1417, 1655, 1742, 1922, 2291
RsaNI	GTAC	8 cut(s)	943, 967, 1129, 1416, 1654, 1741, 1921, 2290
RseI	CAYNNNNRTG	2 cut(s)	1287, 2009
SacI	GAGCTC	2 cut(s)	344, 2004
SalI	GTCGAC	3 cut(s)	1181, 1510, 1895
SapI	GCTCTTC	1 cut(s)	1865
SaqAI	TTAA	3 cut(s)	66, 780, 2299
SatI	GCNGC	7 cut(s)	199, 202, 546, 1527, 1539, 1625, 1876
Sau96I	GGNCC	8 cut(s)	273, 357, 453, 979, 1102, 1548, 1562, 1690
SbfI	CCTGCAGG	1 cut(s)	815
ScaI	AGTACT	1 cut(s)	1655
SchI	GAGTC	5 cut(s)	133, 313, 746, 869, 1911
SdaI	CCTGCAGG	1 cut(s)	815
SfaNI	GCATC	4 cut(s)	609, 1194, 1246, 1957
SfcI	CTRYAG	1 cut(s)	811
Sfr274I	CTCGAG	2 cut(s)	308, 578
SfuI	TTCGAA	1 cut(s)	2220
SgrAI	CRCCGGYG	3 cut(s)	503, 794, 2087
SgrDI	CGTCGACG	1 cut(s)	1895
SinI	GGWCC	6 cut(s)	273, 453, 979, 1102, 1562, 1690
SlaI	CTCGAG	2 cut(s)	308, 578
SmiMI	CAYNNNNRTG	2 cut(s)	1287, 2009
SmlI	CTYRAG	6 cut(s)	13, 308, 335, 578, 911, 1610
SmoI	CTYRAG	6 cut(s)	13, 308, 335, 578, 911, 1610
SphI	GCATGC	1 cut(s)	1003
Sse8387I	CCTGCAGG	1 cut(s)	815
Sse9I	AATT	9 cut(s)	82, 611, 835, 1028, 1092, 1477, 1820, 2122, 2196
SseBI	AGGCCT	1 cut(s)	1910
SspMI	CTAG	6 cut(s)	383, 879, 1238, 1545, 1943, 2037
SstI	GAGCTC	2 cut(s)	344, 2004
StuI	AGGCCT	1 cut(s)	1910
StyI	CCWWGG	5 cut(s)	459, 1339, 1429, 1771, 2255
TaaI	ACNGT	6 cut(s)	649, 790, 1172, 1415, 1799, 2026
TaiI	ACGT	8 cut(s)	36, 56, 105, 120, 948, 1188, 1421, 2214
TasI	AATT	9 cut(s)	82, 611, 835, 1028, 1092, 1477, 1820, 2122, 2196
TatI	WGTACW	4 cut(s)	1653, 1740, 1920, 2289
TauI	GCSGC	5 cut(s)	204, 548, 1529, 1541, 1878
TfiI	GAWTC	3 cut(s)	177, 240, 1882
Tru1I	TTAA	3 cut(s)	66, 780, 2299
Tru9I	TTAA	3 cut(s)	66, 780, 2299
TscAI	CASTG	6 cut(s)	287, 1017, 1278, 1325, 1454, 1871
TseFI	GTSAC	2 cut(s)	872, 2020
TseI	GCWGC	2 cut(s)	198, 1624
Tsp45I	GTSAC	2 cut(s)	872, 2020
TspDTI	ATGAA	5 cut(s)	76, 232, 1321, 1471, 1688
TspGWI	ACGGA	6 cut(s)	394, 504, 1406, 1882, 1914, 2116
TspRI	CASTG	6 cut(s)	287, 1017, 1278, 1325, 1454, 1871
Tth111I	GACNNNGTC	2 cut(s)	536, 1901
Van91I	CCANNNNNTGG	2 cut(s)	282, 1198
VneI	GTGCAC	4 cut(s)	622, 650, 1387, 1445
VpaK11BI	GGWCC	6 cut(s)	273, 453, 979, 1102, 1562, 1690
VspI	ATTAAT	1 cut(s)	780
XagI	CCTNNNNNAGG	2 cut(s)	914, 1273
XapI	RAATTY	3 cut(s)	611, 1477, 1820
XceI	RCATGY	3 cut(s)	1003, 1504, 2080
XhoI	CTCGAG	2 cut(s)	308, 578
XmiI	GTMKAC	3 cut(s)	1182, 1511, 1896
XmnI	GAANNNNTTC	1 cut(s)	1283
XspI	CTAG	6 cut(s)	383, 879, 1238, 1545, 1943, 2037
ZraI	GACGTC	4 cut(s)	34, 54, 118, 2212
ZrmI	AGTACT	1 cut(s)	1655

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.