Rw7G011240

Galactinol--sucrose galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0005392
Physical Location & Seq
Reverse (-)
10517738 .. 10522721
4984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G011240.1

Sequence Viewer

Length: 2319 bp
ATGGCTCCAAGCCTAAGCAAGAACAATGCCGCAGACGTAATGGGCCTTGTAGACAGCAACTCACCACTGTCAATAACACTACAAGGCACAAACTTCCTTGCCAACGGACACCCAATCCTCACAGAAGTCCCACTAAACATCACAGCCACTCCATCAACAAACAAAACCACCGTGGGCTGCTTCATTGGCTTCGACGCCGACGAGCCCAAAAGCCGCCATGTGGCGCCACTCGGCAAGCTCAGCGGCATCAAGTTCATGAGCATTTTCCGGTTCAAAGTCTGGTGGACCACCCACTGGGTCGGAACAACCGGAAACGACATGCAGCACGAGACTCAGTTCATGATCCTCGACAAAAAAGACTCCACCGGCCGCCCCTACGTCCTCCTCCTTCCACTTCTCGAAGGTCCCTTCAGAGCCTCCCTCCAACCCGGCACCGACTGCCACGTTGACATGTGCGTCGAGAGTGGCTCGACACGTGTCAGTGAATCCACCTTCCGGAGCGTTCTCTACATGCACGTCGGCAACGACCCGTACGGTCTTGTCAAAGAGGCCATGGAGGTGGTGAGAGTCCATTTGGGAAGCTTCAAGCTTCTGGAGGAGAAGACGCCTCCGCCTATAGTGGACAAGTTTGGTTGGTGCACGTGGGATGCGTTTTATCTTATGGTGCACCCCAAGGGAGTTTGGGAAGGGGTTAAGGGCCTAGTGGAGGGTGGGTGTCCTCCGGGAATGGTACTCATCGACGACGGCTGGCAGTCCATTTGTCACGATGACGACCCCCTAACCGACGAAGAAGCCATGAAAAGGACATCTGCCGGTGAGCAAATGCCATGTAGGCTCACAAGTTTCGTAGAAAACTACAAGTTCAGAGACTATAAGAGCCCTAGTGGTAAGGGCATGGGTGCCTTTGTTAAGGACTTGAAGGAGGAGTTTGGGAGCGTGGACTATGTTTATGTTTGGCACGCGCTATGTGGGTATTGGGGTGGGGTTAGACCCGGGGTGAAGAGCGGGTCGGGTTTTCCGGAGAGCCGGGTGATTAGGCCGAAGCTGTCGGAGGGTTTGGAGAAGACGATGGAGGATTTGGCGGTGGACAAGATCGTGAACAATGGAGTGGGGCTGGTGGAACCGGAGATGGCCCACAAGTTGTATGAGGGGCTTCATCAGCATCTTCAGTCGGTCGGGATCGACGGTGTCAAGGTCGATGTCATACACTTGCTTGAGATGTTATCCGAGGAGTTTGGTGGTCGGATTGAGCTGGCCAAAGCTTATTACAAGGCACTTACTGCTTCTGTGAGGAAACATTTCAAAGGCAATGGAGTGATTGCTAGCATGGAGCACTGCAATGATTTCATGTATCTTGGCACAGAAGCCATTTCACTTGGTCGTGTTGGAGACGACTTTTGGTGCTCTGACCCGTCTGGAGATCCAAATGGGACATATTGGCTACAAGGGTGTCACATGGTCCATTGTGCCTACAATAGCTTGTGGATGGGGAACTTCGTACATCCAGATTGGGACATGTTTCAGTCGACTCACCCTTGTGCTGCATTTCATGCTGCATCAAGAGCCATATCGGGAGGGCCGATCTATATCAGCGATTCTGTGGGACAGCACAACTTCAAGCTTCTTAAGAGTCTGGTGTTGCCTGATGGATCCATATTAAGATGCCAATACTATGCACTTCCTACTAGGGATTGCCTATTTGAGGACCCTGTTCATGATGGGAAAACAATGCTCAAGATTTGGAACCTCAACAAGTACACCGGCGTTCTAGGACTTTTTAACTGCCAAGGAGGAGGGTGGTGCCCCAAATCAAGGCGCAACAAAAGCTTCCCTGAGTTCTCAAAGTCAGTGACTTGTTTGGCAACCCCAAAAGATGTGGAATGGAGTCATGGGAAGAACCCGATTCCAATAAAAGAAATCGACGTATTTGCTGTGTACATGTTCCAGGAAAAGAAGCTGAAGCTTTTGAAGCCAGCGGATACGGTAGAGATTTCACTTCAGCCTTTCAGTTATGAGCTCCTCACCATTGCTCCAGTGAGAGTGCTACCAACAAAGTTGATCCAATTTGCTCCAATTGGATTGGTGAACATGCTCAACACTGGTGGTGCAATTCAGTCACTAGAATTTGAAGATGGTGAAACTGTCGTGAGGATTGGACTGAAGGGCAGTGGAGAGATGAGAACATTTGCATCAGAAAAACCAACTGCTTGTAAGATTGGTGGAGAGGAAGTGAAATTTGATTATGTTGATAAGATGGTTATTGTCCAAGTGCAATGGCCTAGCTCTTCAATAACAACCGTGGTTGAGTACTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

772

Amino Acids

85.68

Weight (kDa)

5.96

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 30 - 756 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 455
AccB1I GGYRCC 4 cut(s) 223, 431, 899, 1800
AccB7I CCANNNNNTGG 1 cut(s) 294
AccBSI CCGCTC 1 cut(s) 1005
AccI GTMKAC 2 cut(s) 51, 1526
AccII CGCG 1 cut(s) 962
AccIII TCCGGA 2 cut(s) 495, 1018
AciI CCGC 8 cut(s) 30, 214, 243, 370, 611, 1005, 1082, 1976
AclWI GGATC 6 cut(s) 337, 1187, 1415, 1644, 1657, 2053
AcoI YGGCCR 2 cut(s) 367, 1254
AcsI RAATTY 2 cut(s) 2123, 2234
AcuI CTGAAG 5 cut(s) 394, 1151, 1979, 1982, 2180
AcvI CACGTG 2 cut(s) 476, 642
AcyI GRCGYC 3 cut(s) 195, 224, 605
AfaI GTAC 6 cut(s) 533, 732, 1500, 1757, 1937, 2309
AfiI CCNNNNNNNGG 7 cut(s) 220, 294, 495, 706, 801, 1702, 1812
AflII CTTAAG 1 cut(s) 1625
AflIII ACRYGT 5 cut(s) 450, 473, 475, 1515, 1938
AgsI TTSAA 8 cut(s) 274, 586, 919, 1303, 1618, 1969, 2129, 2289
AjiI CACGTC 1 cut(s) 517
AjnI CCWGG 1 cut(s) 1944
AleI CACNNNNGTG 1 cut(s) 1536
Alw21I GWGCWC 5 cut(s) 641, 669, 1335, 1406, 2019
Alw26I GTCTC 3 cut(s) 323, 861, 1383
Alw44I GTGCAC 2 cut(s) 637, 665
AlwI GGATC 6 cut(s) 337, 1187, 1415, 1644, 1657, 2053
Ama87I CYCGRG 1 cut(s) 992
Aor13HI TCCGGA 2 cut(s) 495, 1018
AoxI GGCC 9 cut(s) 43, 367, 549, 697, 1037, 1131, 1254, 1577, 2276
ApaLI GTGCAC 2 cut(s) 637, 665
ApeKI GCWGC 4 cut(s) 177, 322, 1541, 1553
ApoI RAATTY 2 cut(s) 2123, 2234
Asp700I GAANNNNTTC 1 cut(s) 1298
AspLEI GCGC 3 cut(s) 226, 964, 1818
AspS9I GGNCC 8 cut(s) 43, 285, 404, 697, 1132, 1459, 1577, 1705
AsuC2I CCSGG 5 cut(s) 429, 723, 993, 994, 1028
AsuHPI GGTGA 9 cut(s) 54, 574, 827, 1009, 1042, 1523, 2014, 2095, 2147
AsuNHI GCTAGC 1 cut(s) 1322
AvaI CYCGRG 1 cut(s) 992
AvaII GGWCC 4 cut(s) 285, 404, 1459, 1705
BaeGI GKGCMC 3 cut(s) 641, 669, 1805
BalI TGGCCA 1 cut(s) 1256
BamHI GGATCC 1 cut(s) 1649
BanI GGYRCC 4 cut(s) 223, 431, 899, 1800
BanII GRGCYC 3 cut(s) 207, 881, 2019
BauI CACGAG 1 cut(s) 326
BbrPI CACGTG 2 cut(s) 476, 642
BbsI GAAGAC 2 cut(s) 608, 1070
Bbv12I GWGCWC 5 cut(s) 641, 669, 1335, 1406, 2019
BbvI GCAGC 4 cut(s) 164, 334, 1528, 1540
BccI CCATC 8 cut(s) 160, 1063, 1123, 1480, 1640, 1712, 2126, 2248
BceAI ACGGC 1 cut(s) 760
BcgI CGANNNNNNTGC 2 cut(s) 1910, 1944
BciT130I CCWGG 1 cut(s) 1946
BciVI GTATCC 1 cut(s) 1972
BcnI CCSGG 5 cut(s) 429, 723, 993, 994, 1028
BcoDI GTCTC 3 cut(s) 323, 861, 1383
BfaI CTAG 7 cut(s) 701, 882, 1323, 1686, 1769, 2120, 2280
BfmI CTRYAG 1 cut(s) 615
BfoI RGCGCY 1 cut(s) 227
BfrI CTTAAG 1 cut(s) 1625
BfuI GTATCC 1 cut(s) 1972
BglI GCCNNNNNGGC 1 cut(s) 832
BisI GCNGC 8 cut(s) 30, 178, 214, 244, 323, 370, 1542, 1554
BlpI GCTNAGC 1 cut(s) 239
BlsI GCNGC 8 cut(s) 31, 179, 215, 245, 324, 371, 1543, 1555
BmcAI AGTACT 1 cut(s) 2309
Bme1390I CCNGG 6 cut(s) 429, 723, 993, 994, 1028, 1946
Bme18I GGWCC 4 cut(s) 285, 404, 1459, 1705
BmeT110I CYCGRG 1 cut(s) 992
BmgBI CACGTC 1 cut(s) 517
BmgT120I GGNCC 8 cut(s) 43, 285, 404, 697, 1132, 1459, 1577, 1705
BmrFI CCNGG 6 cut(s) 429, 723, 993, 994, 1028, 1946
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 6 cut(s) 255, 637, 1171, 1565, 1652, 2198
BmtI GCTAGC 1 cut(s) 1326
BmuI ACTGGG 1 cut(s) 304
BoxI GACNNNNGTC 1 cut(s) 476
BpiI GAAGAC 2 cut(s) 608, 1070
BplI GAGNNNNNCTC 4 cut(s) 405, 437, 452, 484
BpmI CTGGAG 3 cut(s) 614, 1437, 2016
Bpu10I CCTNAGC 1 cut(s) 14
Bpu1102I GCTNAGC 1 cut(s) 239
BpuEI CTTGAG 2 cut(s) 1235, 1718
BpuMI CCSGG 5 cut(s) 429, 723, 993, 994, 1028
BsaAI YACGTR 2 cut(s) 476, 642
BsaBI GATNNNNATC 1 cut(s) 1586
BsaHI GRCGYC 3 cut(s) 195, 224, 605
BsaJI CCNNGG 8 cut(s) 171, 552, 672, 992, 993, 1227, 1786, 2298
BsaWI WCCGGW 5 cut(s) 267, 308, 495, 1018, 1123
BsaXI ACNNNNNCTCC 8 cut(s) 133, 163, 698, 728, 2014, 2044, 2214, 2244
Bsc4I CCNNNNNNNGG 7 cut(s) 220, 294, 495, 706, 801, 1702, 1812
Bse118I RCCGGY 3 cut(s) 365, 812, 1760
Bse1I ACTGG 3 cut(s) 299, 2033, 2104
Bse3DI GCAATG 4 cut(s) 1315, 1345, 2025, 2279
Bse8I GATNNNNATC 1 cut(s) 1586
BseAI TCCGGA 2 cut(s) 495, 1018
BseBI CCWGG 1 cut(s) 1946
BseDI CCNNGG 8 cut(s) 171, 552, 672, 992, 993, 1227, 1786, 2298
BseGI GGATG 3 cut(s) 652, 1491, 1501
BseJI GATNNNNATC 1 cut(s) 1586
BseLI CCNNNNNNNGG 7 cut(s) 220, 294, 495, 706, 801, 1702, 1812
BseMI GCAATG 4 cut(s) 1315, 1345, 2025, 2279
BseMII CTCAG 3 cut(s) 253, 347, 1824
BseNI ACTGG 3 cut(s) 299, 2033, 2104
BseRI GAGGAG 6 cut(s) 374, 611, 938, 1244, 1806, 2009
BseSI GKGCMC 3 cut(s) 641, 669, 1805
BseX3I CGGCCG 1 cut(s) 367
BseXI GCAGC 4 cut(s) 164, 334, 1528, 1540
Bsh1236I CGCG 1 cut(s) 962
Bsh1285I CGRYCG 2 cut(s) 370, 1176
BshFI GGCC 9 cut(s) 45, 369, 551, 699, 1039, 1133, 1256, 1579, 2278
BshNI GGYRCC 4 cut(s) 223, 431, 899, 1800
BsiEI CGRYCG 2 cut(s) 370, 1176
BsiHKAI GWGCWC 5 cut(s) 641, 669, 1335, 1406, 2019
BsiHKCI CYCGRG 1 cut(s) 992
BsiWI CGTACG 1 cut(s) 531
BslFI GGGAC 5 cut(s) 113, 390, 1444, 1526, 1617
BslI CCNNNNNNNGG 7 cut(s) 220, 294, 495, 706, 801, 1702, 1812
BsmAI GTCTC 3 cut(s) 323, 861, 1383
BsmBI CGTCTC 1 cut(s) 1383
BsmFI GGGAC 5 cut(s) 113, 390, 1444, 1526, 1617
BsnI GGCC 9 cut(s) 45, 369, 551, 699, 1039, 1133, 1256, 1579, 2278
BsoBI CYCGRG 1 cut(s) 992
Bsp1286I GDGCHC 8 cut(s) 207, 641, 669, 881, 1335, 1406, 1805, 2019
Bsp13I TCCGGA 2 cut(s) 495, 1018
Bsp1407I TGTACA 1 cut(s) 1935
Bsp143I GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
Bsp1720I GCTNAGC 1 cut(s) 239
Bsp19I CCATGG 1 cut(s) 552
BspACI CCGC 8 cut(s) 30, 214, 243, 370, 611, 1005, 1082, 1976
BspANI GGCC 9 cut(s) 45, 369, 551, 699, 1039, 1133, 1256, 1579, 2278
BspCNI CTCAG 3 cut(s) 252, 346, 1825
BspEI TCCGGA 2 cut(s) 495, 1018
BspFNI CGCG 1 cut(s) 962
BspHI TCATGA 3 cut(s) 255, 339, 1714
BspOI GCTAGC 1 cut(s) 1326
BspPI GGATC 6 cut(s) 337, 1187, 1415, 1644, 1657, 2053
BspQI GCTCTTC 2 cut(s) 995, 2290
BspT107I GGYRCC 4 cut(s) 223, 431, 899, 1800
BspTI CTTAAG 1 cut(s) 1625
BsrBI CCGCTC 1 cut(s) 1005
BsrDI GCAATG 4 cut(s) 1315, 1345, 2025, 2279
BsrFI RCCGGY 3 cut(s) 365, 812, 1760
BsrGI TGTACA 1 cut(s) 1935
BsrI ACTGG 3 cut(s) 299, 2033, 2104
BssAI RCCGGY 3 cut(s) 365, 812, 1760
BssECI CCNNGG 8 cut(s) 171, 552, 672, 992, 993, 1227, 1786, 2298
BssMI GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
BssNI GRCGYC 3 cut(s) 195, 224, 605
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 3 cut(s) 552, 672, 1786
Bst2BI CACGAG 1 cut(s) 326
Bst2UI CCWGG 1 cut(s) 1946
Bst4CI ACNGT 7 cut(s) 69, 172, 536, 1187, 1984, 2143, 2299
Bst6I CTCTTC 2 cut(s) 995, 2290
BstACI GRCGYC 3 cut(s) 195, 224, 605
BstAFI CTTAAG 1 cut(s) 1625
BstAPI GCANNNNNTGC 3 cut(s) 438, 1280, 1550
BstAUI TGTACA 1 cut(s) 1935
BstBAI YACGTR 2 cut(s) 476, 642
BstC8I GCNNGC 6 cut(s) 236, 749, 960, 1254, 1324, 1974
BstDEI CTNAG 4 cut(s) 14, 239, 333, 1833
BstDSI CCRYGG 3 cut(s) 171, 552, 2298
BstENI CCTNNNNNAGG 2 cut(s) 704, 1700
BstF5I GGATG 3 cut(s) 652, 1491, 1501
BstFNI CGCG 1 cut(s) 962
BstH2I RGCGCY 1 cut(s) 227
BstHHI GCGC 3 cut(s) 226, 964, 1818
BstKTI GATC 7 cut(s) 345, 1095, 1182, 1423, 1584, 1652, 2061
BstMAI GTCTC 3 cut(s) 323, 861, 1383
BstMBI GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
BstMCI CGRYCG 2 cut(s) 370, 1176
BstNI CCWGG 1 cut(s) 1946
BstNSI RCATGY 6 cut(s) 322, 454, 514, 1519, 1942, 2092
BstPAI GACNNNNGTC 1 cut(s) 476
BstSCI CCNGG 6 cut(s) 427, 721, 991, 992, 1026, 1944
BstSFI CTRYAG 1 cut(s) 615
BstSLI GKGCMC 3 cut(s) 641, 669, 1805
BstUI CGCG 1 cut(s) 962
BstV1I GCAGC 4 cut(s) 164, 334, 1528, 1540
BstV2I GAAGAC 2 cut(s) 608, 1070
BstX2I RGATCY 2 cut(s) 1420, 1649
BstXI CCANNNNNNTGG 1 cut(s) 559
BstYI RGATCY 2 cut(s) 1420, 1649
BstZI CGGCCG 1 cut(s) 367
BsuI GTATCC 1 cut(s) 1972
BsuRI GGCC 9 cut(s) 45, 369, 551, 699, 1039, 1133, 1256, 1579, 2278
BtgI CCRYGG 3 cut(s) 171, 552, 2298
BtrI CACGTC 1 cut(s) 517
BtsCI GGATG 3 cut(s) 652, 1491, 1501
BtsI GCAGTG 2 cut(s) 1333, 2173
BtsIMutI CAGTG 8 cut(s) 65, 292, 487, 1333, 1854, 2040, 2097, 2173
Cac8I GCNNGC 6 cut(s) 236, 749, 960, 1254, 1324, 1974
CciI TCATGA 3 cut(s) 255, 339, 1714
CfoI GCGC 3 cut(s) 226, 964, 1818
Cfr10I RCCGGY 3 cut(s) 365, 812, 1760
Cfr13I GGNCC 8 cut(s) 43, 285, 404, 697, 1132, 1459, 1577, 1705
Cfr9I CCCGGG 1 cut(s) 992
CseI GACGC 3 cut(s) 203, 445, 613
Csp6I GTAC 6 cut(s) 532, 731, 1499, 1756, 1936, 2308
CspCI CAANNNNNGTGG 4 cut(s) 1857, 1892, 2083, 2118
CviQI GTAC 6 cut(s) 532, 731, 1499, 1756, 1936, 2308
DdeI CTNAG 4 cut(s) 14, 239, 333, 1833
DinI GGCGCC 1 cut(s) 225
DpnI GATC 7 cut(s) 344, 1094, 1181, 1422, 1583, 1651, 2060
DpnII GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
DrdI GACNNNNNNGTC 1 cut(s) 455
DseDI GACNNNNNNGTC 1 cut(s) 455
EaeI YGGCCR 2 cut(s) 367, 1254
EagI CGGCCG 1 cut(s) 367
Eam1104I CTCTTC 2 cut(s) 995, 2290
EarI CTCTTC 2 cut(s) 995, 2290
EciI GGCGGA 1 cut(s) 600
Ecl136II GAGCTC 1 cut(s) 2017
EclXI CGGCCG 1 cut(s) 367
Eco130I CCWWGG 3 cut(s) 552, 672, 1786
Eco24I GRGCYC 3 cut(s) 207, 881, 2019
Eco47I GGWCC 4 cut(s) 285, 404, 1459, 1705
Eco52I CGGCCG 1 cut(s) 367
Eco53kI GAGCTC 1 cut(s) 2017
Eco57I CTGAAG 5 cut(s) 394, 1151, 1979, 1982, 2180
Eco72I CACGTG 2 cut(s) 476, 642
Eco88I CYCGRG 1 cut(s) 992
EcoICRI GAGCTC 1 cut(s) 2017
EcoNI CCTNNNNNAGG 2 cut(s) 704, 1700
EcoO109I RGGNCCY 3 cut(s) 404, 697, 1705
EcoRII CCWGG 1 cut(s) 1944
EcoT14I CCWWGG 3 cut(s) 552, 672, 1786
EcoT38I GRGCYC 3 cut(s) 207, 881, 2019
EgeI GGCGCC 1 cut(s) 225
EheI GGCGCC 1 cut(s) 225
ErhI CCWWGG 3 cut(s) 552, 672, 1786
Esp3I CGTCTC 1 cut(s) 1383
FaqI GGGAC 5 cut(s) 113, 390, 1444, 1526, 1617
FauI CCCGC 1 cut(s) 998
FblI GTMKAC 2 cut(s) 51, 1526
Fnu4HI GCNGC 8 cut(s) 30, 178, 214, 244, 323, 370, 1542, 1554
FokI GGATG 3 cut(s) 659, 1488, 1498
FriOI GRGCYC 3 cut(s) 207, 881, 2019
Fsp4HI GCNGC 8 cut(s) 30, 178, 214, 244, 323, 370, 1542, 1554
FspBI CTAG 7 cut(s) 701, 882, 1323, 1686, 1769, 2120, 2280
GlaI GCGC 3 cut(s) 225, 963, 1817
GluI GCNGC 8 cut(s) 30, 178, 214, 244, 323, 370, 1542, 1554
GsuI CTGGAG 3 cut(s) 614, 1437, 2016
HaeII RGCGCY 1 cut(s) 227
HaeIII GGCC 9 cut(s) 45, 369, 551, 699, 1039, 1133, 1256, 1579, 2278
HgaI GACGC 3 cut(s) 203, 445, 613
HhaI GCGC 3 cut(s) 226, 964, 1818
Hin1I GRCGYC 3 cut(s) 195, 224, 605
Hin6I GCGC 3 cut(s) 224, 962, 1816
HinP1I GCGC 3 cut(s) 224, 962, 1816
HincII GTYRAC 2 cut(s) 448, 1527
HindII GTYRAC 2 cut(s) 448, 1527
HindIII AAGCTT 6 cut(s) 580, 587, 1260, 1619, 1825, 1961
HinfI GANTC 9 cut(s) 331, 359, 485, 567, 1528, 1595, 1630, 1885, 1903
HphI GGTGA 9 cut(s) 54, 574, 827, 1009, 1042, 1523, 2014, 2095, 2147
Hpy188I TCNGA 8 cut(s) 302, 413, 866, 1051, 1228, 1245, 1408, 2194
Hpy99I CGWCG 9 cut(s) 197, 203, 461, 521, 743, 746, 788, 1187, 1925
HpyAV CCTTC 7 cut(s) 395, 398, 418, 502, 680, 913, 2155
HpyCH4III ACNGT 7 cut(s) 69, 172, 536, 1187, 1984, 2143, 2299
HpyCH4IV ACGT 7 cut(s) 36, 378, 444, 475, 516, 641, 1923
HpyF3I CTNAG 4 cut(s) 14, 239, 333, 1833
HpySE526I ACGT 7 cut(s) 36, 378, 444, 475, 516, 641, 1923
Hsp92I GRCGYC 3 cut(s) 195, 224, 605
HspAI GCGC 3 cut(s) 224, 962, 1816
KasI GGCGCC 1 cut(s) 223
Kpn2I TCCGGA 2 cut(s) 495, 1018
Kzo9I GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
LguI GCTCTTC 2 cut(s) 995, 2290
LmnI GCTCC 7 cut(s) 10, 498, 933, 1330, 2022, 2035, 2074
Lsp1109I GCAGC 4 cut(s) 164, 334, 1528, 1540
LweI GCATC 6 cut(s) 255, 637, 1171, 1565, 1652, 2198
MaeI CTAG 7 cut(s) 701, 882, 1323, 1686, 1769, 2120, 2280
MaeII ACGT 7 cut(s) 36, 378, 444, 475, 516, 641, 1923
MaeIII GTNAC 4 cut(s) 761, 1451, 1849, 2115
MalI GATC 7 cut(s) 344, 1094, 1181, 1422, 1583, 1651, 2060
MbiI CCGCTC 1 cut(s) 1005
MboI GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
MboII GAAGA 8 cut(s) 613, 800, 1012, 1075, 1157, 1906, 2141, 2277
MfeI CAATTG 1 cut(s) 2073
MflI RGATCY 2 cut(s) 1420, 1649
MhlI GDGCHC 8 cut(s) 207, 641, 669, 881, 1335, 1406, 1805, 2019
MlsI TGGCCA 1 cut(s) 1256
MluCI AATT 5 cut(s) 2063, 2073, 2109, 2123, 2234
MluNI TGGCCA 1 cut(s) 1256
Mly113I GGCGCC 1 cut(s) 224
MlyI GAGTC 6 cut(s) 325, 353, 576, 1522, 1639, 1894
MmeI TCCRAC 5 cut(s) 280, 448, 1029, 1223, 1366
Mox20I TGGCCA 1 cut(s) 1256
MroI TCCGGA 2 cut(s) 495, 1018
MroXI GAANNNNTTC 1 cut(s) 1298
MscI TGGCCA 1 cut(s) 1256
MseI TTAA 5 cut(s) 693, 909, 1626, 1658, 1779
MslI CAYNNNNRTG 3 cut(s) 557, 1338, 1536
Msp20I TGGCCA 1 cut(s) 1256
MspA1I CMGCKG 2 cut(s) 243, 1976
MspCI CTTAAG 1 cut(s) 1625
MspR9I CCNGG 6 cut(s) 429, 723, 993, 994, 1028, 1946
MunI CAATTG 1 cut(s) 2073
MvaI CCWGG 1 cut(s) 1946
MvnI CGCG 1 cut(s) 962
NarI GGCGCC 1 cut(s) 224
NciI CCSGG 5 cut(s) 429, 723, 993, 994, 1028
NcoI CCATGG 1 cut(s) 552
NdeII GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
NheI GCTAGC 1 cut(s) 1322
NmeAIII GCCGAG 1 cut(s) 210
NmuCI GTSAC 4 cut(s) 761, 1451, 1849, 2115
NspI RCATGY 6 cut(s) 322, 454, 514, 1519, 1942, 2092
OliI CACNNNNGTG 1 cut(s) 1536
PagI TCATGA 3 cut(s) 255, 339, 1714
PciI ACATGT 3 cut(s) 450, 1515, 1938
PciSI GCTCTTC 2 cut(s) 995, 2290
PcsI WCGNNNNNNNCGW 4 cut(s) 198, 522, 647, 1577
PdmI GAANNNNTTC 1 cut(s) 1298
PfeI GAWTC 3 cut(s) 485, 1595, 1903
Pfl23II CGTACG 1 cut(s) 531
PflFI GACNNNGTC 1 cut(s) 1187
PflMI CCANNNNNTGG 1 cut(s) 294
PfoI TCCNGGA 2 cut(s) 721, 1944
PkrI GCNGC 8 cut(s) 31, 179, 215, 245, 324, 371, 1543, 1555
PleI GAGTC 6 cut(s) 325, 353, 575, 1522, 1638, 1893
PluTI GGCGCC 1 cut(s) 227
PmaCI CACGTG 2 cut(s) 476, 642
PmlI CACGTG 2 cut(s) 476, 642
PpsI GAGTC 6 cut(s) 325, 353, 575, 1522, 1638, 1893
Ppu21I YACGTR 2 cut(s) 476, 642
PpuMI RGGWCCY 2 cut(s) 404, 1705
PscI ACATGT 3 cut(s) 450, 1515, 1938
PshAI GACNNNNGTC 1 cut(s) 476
Psp124BI GAGCTC 1 cut(s) 2019
Psp5II RGGWCCY 2 cut(s) 404, 1705
Psp6I CCWGG 1 cut(s) 1944
PspCI CACGTG 2 cut(s) 476, 642
PspGI CCWGG 1 cut(s) 1944
PspLI CGTACG 1 cut(s) 531
PspPI GGNCC 8 cut(s) 43, 285, 404, 697, 1132, 1459, 1577, 1705
PspPPI RGGWCCY 2 cut(s) 404, 1705
PsuI RGATCY 2 cut(s) 1420, 1649
PsyI GACNNNGTC 1 cut(s) 1187
RsaI GTAC 6 cut(s) 533, 732, 1500, 1757, 1937, 2309
RsaNI GTAC 6 cut(s) 532, 731, 1499, 1756, 1936, 2308
RseI CAYNNNNRTG 3 cut(s) 557, 1338, 1536
SacI GAGCTC 1 cut(s) 2019
SalI GTCGAC 1 cut(s) 1525
SapI GCTCTTC 2 cut(s) 995, 2290
SaqAI TTAA 5 cut(s) 693, 909, 1626, 1658, 1779
SatI GCNGC 8 cut(s) 30, 178, 214, 244, 323, 370, 1542, 1554
Sau3AI GATC 7 cut(s) 342, 1092, 1179, 1420, 1581, 1649, 2058
Sau96I GGNCC 8 cut(s) 43, 285, 404, 697, 1132, 1459, 1577, 1705
ScaI AGTACT 1 cut(s) 2309
SchI GAGTC 6 cut(s) 325, 353, 576, 1522, 1639, 1894
ScrFI CCNGG 6 cut(s) 429, 723, 993, 994, 1028, 1946
SduI GDGCHC 8 cut(s) 207, 641, 669, 881, 1335, 1406, 1805, 2019
SfaNI GCATC 6 cut(s) 255, 637, 1171, 1565, 1652, 2198
SfcI CTRYAG 1 cut(s) 615
SfoI GGCGCC 1 cut(s) 225
SgrAI CRCCGGYG 1 cut(s) 1760
SinI GGWCC 4 cut(s) 285, 404, 1459, 1705
SmaI CCCGGG 1 cut(s) 994
SmiMI CAYNNNNRTG 3 cut(s) 557, 1338, 1536
SmlI CTYRAG 3 cut(s) 1214, 1625, 1733
SmoI CTYRAG 3 cut(s) 1214, 1625, 1733
Sse9I AATT 5 cut(s) 2063, 2073, 2109, 2123, 2234
SsiI CCGC 8 cut(s) 30, 214, 243, 370, 611, 1005, 1082, 1976
SspDI GGCGCC 1 cut(s) 223
SspMI CTAG 7 cut(s) 701, 882, 1323, 1686, 1769, 2120, 2280
SstI GAGCTC 1 cut(s) 2019
StyD4I CCNGG 6 cut(s) 427, 721, 991, 992, 1026, 1944
StyI CCWWGG 3 cut(s) 552, 672, 1786
TaaI ACNGT 7 cut(s) 69, 172, 536, 1187, 1984, 2143, 2299
TaiI ACGT 7 cut(s) 39, 381, 447, 478, 519, 644, 1926
TaqII GACCGA 1 cut(s) 1162
TasI AATT 5 cut(s) 2063, 2073, 2109, 2123, 2234
TatI WGTACW 3 cut(s) 1755, 1935, 2307
TauI GCSGC 4 cut(s) 32, 216, 246, 372
TfiI GAWTC 3 cut(s) 485, 1595, 1903
Tru1I TTAA 5 cut(s) 693, 909, 1626, 1658, 1779
Tru9I TTAA 5 cut(s) 693, 909, 1626, 1658, 1779
TscAI CASTG 8 cut(s) 72, 299, 487, 1340, 1854, 2040, 2104, 2173
TseFI GTSAC 4 cut(s) 761, 1451, 1849, 2115
TseI GCWGC 4 cut(s) 177, 322, 1541, 1553
Tsp45I GTSAC 4 cut(s) 761, 1451, 1849, 2115
TspDTI ATGAA 8 cut(s) 172, 244, 328, 812, 1145, 1336, 1538, 1703
TspGWI ACGGA 1 cut(s) 120
TspMI CCCGGG 1 cut(s) 992
TspRI CASTG 8 cut(s) 72, 299, 487, 1340, 1854, 2040, 2104, 2173
Tth111I GACNNNGTC 1 cut(s) 1187
Van91I CCANNNNNTGG 1 cut(s) 294
Vha464I CTTAAG 1 cut(s) 1625
VneI GTGCAC 2 cut(s) 637, 665
VpaK11BI GGWCC 4 cut(s) 285, 404, 1459, 1705
XagI CCTNNNNNAGG 2 cut(s) 704, 1700
XapI RAATTY 2 cut(s) 2123, 2234
XceI RCATGY 6 cut(s) 322, 454, 514, 1519, 1942, 2092
XmaI CCCGGG 1 cut(s) 992
XmiI GTMKAC 2 cut(s) 51, 1526
XmnI GAANNNNTTC 1 cut(s) 1298
XspI CTAG 7 cut(s) 701, 882, 1323, 1686, 1769, 2120, 2280
ZrmI AGTACT 1 cut(s) 2309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.