FvH4_5g09520

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012764
Physical Location & Seq
Forward (+)
5523759 .. 5525461
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g09520.t1

Sequence Viewer

Length: 768 bp
ATGGTATCGGCTCTAACTCAGGTGATTGGAAACACTACTCATGAACAAAATCCGCTTCAAGTTATTGATCATCAAGTTCTTGGAAACCCCCAAGTCTTTTCTGCAGAAAACCCTGTTGAGCAATCTCAACCTGCAGTAGTTCTTCAAGCTCAAGGAAATGTGAGACAACACTATAGGGGAGTGAGGCGGAGGCCATGGGGGAAGTGGGCAGCCGAGATTCGTGATCCACATAAAGCAGCCAGAGTGTGGCTCGGGACTTTTGAAACCGCGGAGGCTGCTGCCCTTGCTTATGATGAAGCAGCTCTCAGATTTAAAGGAAGCAAGGCTAAGCTTAACTTCCCCGAAAGAGTTCAAGGGATCAGCCCTAGTACTTCTAGTGGTCTCTACCTTGCAATAAGTACTAGTACCGGTCATGATCATCGACTAGCCAATTCAGCTCCTCCAGCTGCTCCAATTTCTCGACCAACAACATATAGTATTAATCCAAATAATGTTAATATTGATTATGATGCACTTGTATCATCGTCTCAAAACCACGGACAAGAAAGAACTATGCCGCAGATTAATGTACAAACGACGTTGTCTTCTACATCCTCGGCCTCTTCCATGCCATCTCATCATCAACAAGAACGAGTACAGCAGCAAGAAGATCAGCAGCAGCTTTTAAAGTTTCCCATTATGCCATTTGGCGGTTCGTCTTCGAGTTCATCAGATCCTCCTACTAAGTACAGGAGAGACTCCTCTGGGAGCGGTGACTTTAGAAGATGA

Protein Analysis

256

Amino Acids

27.95

Weight (kDa)

9.25

Isoelectric Point (pI)

63.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 57 - 106 3.1e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012764)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07310 AT5G61890
fragaria_vesca FvH4_5g09520
malus_domestica MD06G1130100.v1.1 MD06G1130300.v1.1 MD14G1147100.v1.1
prunus_persica Prupe.5G141200_v2.0.a1
pyrus_communis pycom14g12170
rosa_chinensis RchiOBHm_Chr7g0188681
rosa_laevigata RLG00000004671
rosa_multiflora Rmu_sc0001576.1_g000027
rosa_roxburghii Rroxscaffold_3G00266140
rosa_rugosa Rorug06G0492200
rosa_samantha Rh7AG097100 Rh7BG098900 Rh7CG100700 Rh7DG099300
rosa_wichuraiana Rw7G008400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 139
AccB7I CCANNNNNTGG 1 cut(s) 246
AccBSI CCGCTC 1 cut(s) 750
AccII CGCG 1 cut(s) 269
AciI CCGC 7 cut(s) 53, 187, 267, 269, 557, 690, 750
AclWI GGATC 3 cut(s) 218, 365, 707
AfaI GTAC 6 cut(s) 370, 400, 406, 570, 636, 728
AfiI CCNNNNNNNGG 2 cut(s) 246, 689
AgeI ACCGGT 1 cut(s) 407
AgsI TTSAA 4 cut(s) 59, 146, 263, 353
AhlI ACTAGT 1 cut(s) 401
AluBI AGCT 6 cut(s) 149, 302, 331, 437, 446, 661
AluI AGCT 6 cut(s) 149, 302, 331, 437, 446, 661
Alw26I GTCTC 4 cut(s) 157, 386, 531, 729
AlwI GGATC 3 cut(s) 218, 365, 707
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 2 cut(s) 191, 597
ApeKI GCWGC 9 cut(s) 209, 236, 275, 278, 299, 446, 640, 655, 658
AseI ATTAAT 2 cut(s) 480, 564
AsiGI ACCGGT 1 cut(s) 407
Asp700I GAANNNNTTC 1 cut(s) 348
AsuHPI GGTGA 2 cut(s) 34, 764
AvaI CYCGRG 1 cut(s) 251
BbsI GAAGAC 2 cut(s) 576, 690
BbvI GCAGC 9 cut(s) 221, 248, 262, 265, 311, 433, 652, 667, 670
BccI CCATC 1 cut(s) 619
BclI TGATCA 2 cut(s) 67, 415
BcoDI GTCTC 4 cut(s) 157, 386, 531, 729
BcuI ACTAGT 1 cut(s) 401
BfaI CTAG 4 cut(s) 366, 375, 402, 425
BfmI CTRYAG 3 cut(s) 102, 132, 172
BfuAI ACCTGC 1 cut(s) 139
BlpI GCTNAGC 1 cut(s) 327
BmcAI AGTACT 2 cut(s) 370, 400
BmeT110I CYCGRG 1 cut(s) 251
BmsI GCATC 1 cut(s) 499
BpiI GAAGAC 2 cut(s) 576, 690
BplI GAGNNNNNCTC 2 cut(s) 234, 266
BpmI CTGGAG 1 cut(s) 426
Bpu1102I GCTNAGC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 4 cut(s) 194, 267, 535, 594
BsaWI WCCGGW 1 cut(s) 407
Bsc4I CCNNNNNNNGG 2 cut(s) 246, 689
Bse118I RCCGGY 1 cut(s) 407
BseDI CCNNGG 4 cut(s) 194, 267, 535, 594
BseGI GGATG 1 cut(s) 590
BseLI CCNNNNNNNGG 2 cut(s) 246, 689
BseMII CTCAG 2 cut(s) 32, 319
BseRI GAGGAG 2 cut(s) 429, 730
BseXI GCAGC 9 cut(s) 221, 248, 262, 265, 311, 433, 652, 667, 670
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 2 cut(s) 193, 599
BshTI ACCGGT 1 cut(s) 407
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 1 cut(s) 408
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 2 cut(s) 246, 689
BsmAI GTCTC 4 cut(s) 157, 386, 531, 729
BsmBI CGTCTC 1 cut(s) 531
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 2 cut(s) 193, 599
Bso31I GGTCTC 1 cut(s) 386
BsoBI CYCGRG 1 cut(s) 251
Bsp1407I TGTACA 1 cut(s) 568
Bsp143I GATC 6 cut(s) 67, 223, 357, 415, 649, 712
Bsp1720I GCTNAGC 1 cut(s) 327
Bsp19I CCATGG 1 cut(s) 194
BspACI CCGC 7 cut(s) 53, 187, 267, 269, 557, 690, 750
BspANI GGCC 2 cut(s) 193, 599
BspCNI CTCAG 2 cut(s) 31, 318
BspFNI CGCG 1 cut(s) 269
BspHI TCATGA 2 cut(s) 40, 412
BspMAI CTGCAG 2 cut(s) 106, 136
BspMI ACCTGC 1 cut(s) 139
BspPI GGATC 3 cut(s) 218, 365, 707
BspTNI GGTCTC 1 cut(s) 386
BsrBI CCGCTC 1 cut(s) 750
BsrFI RCCGGY 1 cut(s) 407
BsrGI TGTACA 1 cut(s) 568
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 4 cut(s) 194, 267, 535, 594
BssMI GATC 6 cut(s) 67, 223, 357, 415, 649, 712
BssT1I CCWWGG 1 cut(s) 194
Bst6I CTCTTC 1 cut(s) 607
BstAUI TGTACA 1 cut(s) 568
BstDEI CTNAG 4 cut(s) 18, 305, 327, 723
BstDSI CCRYGG 3 cut(s) 194, 267, 535
BstF5I GGATG 1 cut(s) 590
BstFNI CGCG 1 cut(s) 269
BstKTI GATC 6 cut(s) 70, 226, 360, 418, 652, 715
BstMAI GTCTC 4 cut(s) 157, 386, 531, 729
BstMBI GATC 6 cut(s) 67, 223, 357, 415, 649, 712
BstMWI GCNNNNNNNGC 4 cut(s) 275, 284, 434, 443
BstSFI CTRYAG 3 cut(s) 102, 132, 172
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 9 cut(s) 221, 248, 262, 265, 311, 433, 652, 667, 670
BstV2I GAAGAC 2 cut(s) 576, 690
BstX2I RGATCY 1 cut(s) 712
BstYI RGATCY 1 cut(s) 712
BsuRI GGCC 2 cut(s) 193, 599
BtgI CCRYGG 3 cut(s) 194, 267, 535
BtsCI GGATG 1 cut(s) 590
BveI ACCTGC 1 cut(s) 139
CciI TCATGA 2 cut(s) 40, 412
Cfr10I RCCGGY 1 cut(s) 407
Cfr42I CCGCGG 1 cut(s) 270
Csp6I GTAC 6 cut(s) 369, 399, 405, 569, 635, 727
CspAI ACCGGT 1 cut(s) 407
CviAII CATG 4 cut(s) 41, 195, 413, 607
CviQI GTAC 6 cut(s) 369, 399, 405, 569, 635, 727
DdeI CTNAG 4 cut(s) 18, 305, 327, 723
DpnI GATC 6 cut(s) 69, 225, 359, 417, 651, 714
DpnII GATC 6 cut(s) 67, 223, 357, 415, 649, 712
DraI TTTAAA 2 cut(s) 313, 666
Eam1104I CTCTTC 1 cut(s) 607
EarI CTCTTC 1 cut(s) 607
EciI GGCGGA 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 386
Eco88I CYCGRG 1 cut(s) 251
EcoT14I CCWWGG 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 194
Esp3I CGTCTC 1 cut(s) 531
FaeI CATG 4 cut(s) 44, 198, 416, 610
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FaqI GGGAC 1 cut(s) 268
FatI CATG 4 cut(s) 40, 194, 412, 606
FbaI TGATCA 2 cut(s) 67, 415
FokI GGATG 1 cut(s) 577
FspBI CTAG 4 cut(s) 366, 375, 402, 425
GsuI CTGGAG 1 cut(s) 426
HaeIII GGCC 2 cut(s) 193, 599
HapII CCGG 1 cut(s) 408
Hin1II CATG 4 cut(s) 44, 198, 416, 610
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 2 cut(s) 217, 737
HpaII CCGG 1 cut(s) 408
HphI GGTGA 2 cut(s) 34, 764
Hpy188I TCNGA 2 cut(s) 308, 712
Hpy188III TCNNGA 5 cut(s) 41, 221, 253, 413, 459
Hpy99I CGWCG 1 cut(s) 580
HpyCH4IV ACGT 1 cut(s) 578
HpyCH4V TGCA 4 cut(s) 104, 134, 392, 512
HpyF10VI GCNNNNNNNGC 4 cut(s) 275, 284, 434, 443
HpyF3I CTNAG 4 cut(s) 18, 305, 327, 723
HpySE526I ACGT 1 cut(s) 578
Hsp92II CATG 4 cut(s) 44, 198, 416, 610
Ksp22I TGATCA 2 cut(s) 67, 415
KspI CCGCGG 1 cut(s) 270
Kzo9I GATC 6 cut(s) 67, 223, 357, 415, 649, 712
LmnI GCTCC 3 cut(s) 442, 454, 747
LpnPI CCDG 8 cut(s) 5, 126, 144, 253, 421, 456, 715, 729
Lsp1109I GCAGC 9 cut(s) 221, 248, 262, 265, 311, 433, 652, 667, 670
LweI GCATC 1 cut(s) 499
MaeI CTAG 4 cut(s) 366, 375, 402, 425
MaeII ACGT 1 cut(s) 578
MaeIII GTNAC 1 cut(s) 752
MalI GATC 6 cut(s) 69, 225, 359, 417, 651, 714
MbiI CCGCTC 1 cut(s) 750
MboI GATC 6 cut(s) 67, 223, 357, 415, 649, 712
MboII GAAGA 5 cut(s) 134, 576, 594, 659, 690
MflI RGATCY 1 cut(s) 712
MluCI AATT 2 cut(s) 430, 453
MlyI GAGTC 1 cut(s) 731
MnlI CCTC 8 cut(s) 177, 183, 265, 450, 604, 610, 726, 751
MroXI GAANNNNTTC 1 cut(s) 348
MseI TTAA 6 cut(s) 312, 333, 480, 495, 564, 665
MspA1I CMGCKG 2 cut(s) 269, 446
MspI CCGG 1 cut(s) 408
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 4 cut(s) 275, 284, 434, 443
NcoI CCATGG 1 cut(s) 194
NdeII GATC 6 cut(s) 67, 223, 357, 415, 649, 712
NlaIII CATG 4 cut(s) 44, 198, 416, 610
NmeAIII GCCGAG 2 cut(s) 238, 575
NmuCI GTSAC 1 cut(s) 752
PagI TCATGA 2 cut(s) 40, 412
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 217
PflFI GACNNNGTC 1 cut(s) 580
PflMI CCANNNNNTGG 1 cut(s) 246
PinAI ACCGGT 1 cut(s) 407
PleI GAGTC 1 cut(s) 731
PpsI GAGTC 1 cut(s) 731
PshBI ATTAAT 2 cut(s) 480, 564
PstI CTGCAG 2 cut(s) 106, 136
PsuI RGATCY 1 cut(s) 712
PsyI GACNNNGTC 1 cut(s) 580
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 6 cut(s) 370, 400, 406, 570, 636, 728
RsaNI GTAC 6 cut(s) 369, 399, 405, 569, 635, 727
SacII CCGCGG 1 cut(s) 270
SaqAI TTAA 6 cut(s) 312, 333, 480, 495, 564, 665
Sau3AI GATC 6 cut(s) 67, 223, 357, 415, 649, 712
ScaI AGTACT 2 cut(s) 370, 400
SchI GAGTC 1 cut(s) 731
SfaNI GCATC 1 cut(s) 499
SfcI CTRYAG 3 cut(s) 102, 132, 172
Sfr303I CCGCGG 1 cut(s) 270
SgrBI CCGCGG 1 cut(s) 270
SmlI CTYRAG 1 cut(s) 150
SmoI CTYRAG 1 cut(s) 150
SpeI ACTAGT 1 cut(s) 401
Sse9I AATT 2 cut(s) 430, 453
SsiI CCGC 7 cut(s) 53, 187, 267, 269, 557, 690, 750
SspI AATATT 1 cut(s) 499
SspMI CTAG 4 cut(s) 366, 375, 402, 425
StyI CCWWGG 1 cut(s) 194
TaiI ACGT 1 cut(s) 581
TaqI TCGA 3 cut(s) 421, 460, 701
TasI AATT 2 cut(s) 430, 453
TatI WGTACW 5 cut(s) 368, 398, 568, 634, 726
TauI GCSGC 1 cut(s) 559
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 6 cut(s) 312, 333, 480, 495, 564, 665
Tru9I TTAA 6 cut(s) 312, 333, 480, 495, 564, 665
TseFI GTSAC 1 cut(s) 752
TseI GCWGC 9 cut(s) 209, 236, 275, 278, 299, 446, 640, 655, 658
Tsp45I GTSAC 1 cut(s) 752
TspDTI ATGAA 3 cut(s) 57, 309, 696
TspGWI ACGGA 1 cut(s) 552
Tth111I GACNNNGTC 1 cut(s) 580
Van91I CCANNNNNTGG 1 cut(s) 246
VspI ATTAAT 2 cut(s) 480, 564
XcmI CCANNNNNNNNNTGG 1 cut(s) 201
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 4 cut(s) 366, 375, 402, 425
ZrmI AGTACT 2 cut(s) 370, 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.