MD14G1147100.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012764
Physical Location & Seq
Reverse (-)
23978106 .. 23980121
2016 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1147100.v1.1.491

Sequence Viewer

Length: 630 bp
ATGGTTTCAGCTCTAGCTCAGGTGATCGGAAATAATAGTGACCAAATTAATAACCCACTTGATCAAGTGCAAGGAATAAGTTCGTTAATCACCTCACAGTCTAGCCCTACCGAGACTCAATCTCAACCAGTACTACTTCAAGATCAAGGGAATTTGAGGAGACAACACTATAGAGGAGTGAGGCGGAGACCATGGGGAAAGTGGGCGGCGGAGATTCGTGATCCGATTAAGGCAGCCCGAGTGTGGCTCGGCACTTTCAACACAGCCGAGGCTGCAGCTCTAGCTTATGATGGAGCTGCTCTCAGGTTCAAAGGAAGCAAAGCTAAGCTCAACTTCCCCGAAAGAGTAGTTCAAGGGAGCTCTGAGTCAGGTTGTCTCACAATAACTACTCAATTACAACACAGCTTGAACAATATTCCTCCTGAGGCTAATATTTCTCGACCAACATATTCCAATGTTTTCGATTATGCACGATATAATAATGTCACATCTACATCGTCGTCCTCCATGCCATCTCAACAAGCCGCAGAGCTCCGAAGCTTTTCGATGCAGTTTGGTTCTTCTTCAAGTTCGAGTTCGGGTCCTCCTTATAAGTATAGGAAAGACTTTGACAGAAGTCACTCAAGATGA

Protein Analysis

210

Amino Acids

23.03

Weight (kDa)

9.88

Isoelectric Point (pI)

66.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 56 - 105 3e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012764)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07310 AT5G61890
fragaria_vesca FvH4_5g09520
malus_domestica MD06G1130100.v1.1 MD06G1130300.v1.1 MD14G1147100.v1.1
prunus_persica Prupe.5G141200_v2.0.a1
pyrus_communis pycom14g12170
rosa_chinensis RchiOBHm_Chr7g0188681
rosa_laevigata RLG00000004671
rosa_multiflora Rmu_sc0001576.1_g000027
rosa_roxburghii Rroxscaffold_3G00266140
rosa_rugosa Rorug06G0492200
rosa_samantha Rh7AG097100 Rh7BG098900 Rh7CG100700 Rh7DG099300
rosa_wichuraiana Rw7G008400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 591
AciI CCGC 4 cut(s) 184, 206, 209, 525
AclWI GGATC 1 cut(s) 215
AcsI RAATTY 1 cut(s) 151
AfaI GTAC 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 243
AgsI TTSAA 6 cut(s) 140, 259, 310, 353, 409, 567
Alw21I GWGCWC 2 cut(s) 362, 534
Alw26I GTCTC 4 cut(s) 107, 154, 181, 380
AlwI GGATC 1 cut(s) 215
Ama87I CYCGRG 1 cut(s) 237
ApeKI GCWGC 4 cut(s) 233, 272, 275, 296
ApoI RAATTY 1 cut(s) 151
AseI ATTAAT 1 cut(s) 48
Asp700I GAANNNNTTC 2 cut(s) 79, 541
AspS9I GGNCC 1 cut(s) 581
AsuHPI GGTGA 2 cut(s) 34, 82
AvaI CYCGRG 1 cut(s) 237
AvaII GGWCC 1 cut(s) 581
AxyI CCTNAGG 1 cut(s) 423
BanII GRGCYC 2 cut(s) 362, 534
Bbv12I GWGCWC 2 cut(s) 362, 534
BbvI GCAGC 4 cut(s) 245, 259, 283, 287
BccI CCATC 2 cut(s) 284, 520
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 4 cut(s) 107, 154, 181, 380
BfaI CTAG 3 cut(s) 14, 102, 281
BfmI CTRYAG 2 cut(s) 169, 273
BisI GCNGC 6 cut(s) 207, 234, 273, 276, 297, 525
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 6 cut(s) 208, 235, 274, 277, 298, 526
BmcAI AGTACT 1 cut(s) 132
Bme18I GGWCC 1 cut(s) 581
BmeT110I CYCGRG 1 cut(s) 237
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 1 cut(s) 582
BmsI GCATC 1 cut(s) 537
BoxI GACNNNNGTC 1 cut(s) 615
BplI GAGNNNNNCTC 4 cut(s) 231, 263, 285, 317
Bpu10I CCTNAGC 1 cut(s) 18
Bpu1102I GCTNAGC 1 cut(s) 324
BpuEI CTTGAG 1 cut(s) 607
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 2 cut(s) 191, 267
Bsc4I CCNNNNNNNGG 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 128
Bse21I CCTNAGG 1 cut(s) 423
BseDI CCNNGG 2 cut(s) 191, 267
BseLI CCNNNNNNNGG 1 cut(s) 243
BseMII CTCAG 4 cut(s) 32, 316, 354, 414
BseNI ACTGG 1 cut(s) 128
BseRI GAGGAG 2 cut(s) 172, 189
BseXI GCAGC 4 cut(s) 245, 259, 283, 287
BsiHKAI GWGCWC 2 cut(s) 362, 534
BsiHKCI CYCGRG 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 243
BsmAI GTCTC 4 cut(s) 107, 154, 181, 380
Bso31I GGTCTC 1 cut(s) 181
BsoBI CYCGRG 1 cut(s) 237
Bsp1286I GDGCHC 2 cut(s) 362, 534
Bsp143I GATC 4 cut(s) 24, 61, 142, 220
Bsp1720I GCTNAGC 1 cut(s) 324
Bsp19I CCATGG 1 cut(s) 191
BspACI CCGC 4 cut(s) 184, 206, 209, 525
BspCNI CTCAG 4 cut(s) 31, 315, 355, 415
BspLI GGNNCC 1 cut(s) 582
BspMAI CTGCAG 1 cut(s) 277
BspPI GGATC 1 cut(s) 215
BspTNI GGTCTC 1 cut(s) 181
BsrI ACTGG 1 cut(s) 128
BssECI CCNNGG 2 cut(s) 191, 267
BssMI GATC 4 cut(s) 24, 61, 142, 220
BssT1I CCWWGG 1 cut(s) 191
Bst4CI ACNGT 1 cut(s) 99
BstDEI CTNAG 5 cut(s) 18, 302, 324, 363, 423
BstDSI CCRYGG 1 cut(s) 191
BstKTI GATC 4 cut(s) 27, 64, 145, 223
BstMAI GTCTC 4 cut(s) 107, 154, 181, 380
BstMBI GATC 4 cut(s) 24, 61, 142, 220
BstMWI GCNNNNNNNGC 2 cut(s) 272, 281
BstPAI GACNNNNGTC 1 cut(s) 615
BstSFI CTRYAG 2 cut(s) 169, 273
BstV1I GCAGC 4 cut(s) 245, 259, 283, 287
Bsu36I CCTNAGG 1 cut(s) 423
BtgI CCRYGG 1 cut(s) 191
Cfr13I GGNCC 1 cut(s) 581
Csp6I GTAC 1 cut(s) 131
CviAII CATG 2 cut(s) 192, 508
CviQI GTAC 1 cut(s) 131
DdeI CTNAG 5 cut(s) 18, 302, 324, 363, 423
DpnI GATC 4 cut(s) 26, 63, 144, 222
DpnII GATC 4 cut(s) 24, 61, 142, 220
EciI GGCGGA 2 cut(s) 199, 224
Ecl136II GAGCTC 2 cut(s) 360, 532
Eco130I CCWWGG 1 cut(s) 191
Eco24I GRGCYC 2 cut(s) 362, 534
Eco31I GGTCTC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 581
Eco53kI GAGCTC 2 cut(s) 360, 532
Eco81I CCTNAGG 1 cut(s) 423
Eco88I CYCGRG 1 cut(s) 237
EcoICRI GAGCTC 2 cut(s) 360, 532
EcoO109I RGGNCCY 1 cut(s) 581
EcoT14I CCWWGG 1 cut(s) 191
EcoT38I GRGCYC 2 cut(s) 362, 534
ErhI CCWWGG 1 cut(s) 191
FaeI CATG 2 cut(s) 195, 511
FaiI YATR 9 cut(s) 171, 193, 288, 448, 468, 477, 509, 591, 597
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FatI CATG 2 cut(s) 191, 507
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 6 cut(s) 207, 234, 273, 276, 297, 525
FriOI GRGCYC 2 cut(s) 362, 534
Fsp4HI GCNGC 6 cut(s) 207, 234, 273, 276, 297, 525
FspBI CTAG 3 cut(s) 14, 102, 281
GluI GCNGC 6 cut(s) 207, 234, 273, 276, 297, 525
Hin1II CATG 2 cut(s) 195, 511
HindIII AAGCTT 1 cut(s) 538
HinfI GANTC 3 cut(s) 115, 214, 365
HphI GGTGA 2 cut(s) 34, 82
Hpy188I TCNGA 4 cut(s) 29, 225, 364, 536
Hpy188III TCNNGA 5 cut(s) 140, 218, 422, 438, 624
Hpy99I CGWCG 1 cut(s) 502
HpyCH4III ACNGT 1 cut(s) 99
HpyCH4V TGCA 4 cut(s) 70, 275, 470, 550
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 281
HpyF3I CTNAG 5 cut(s) 18, 302, 324, 363, 423
Hsp92II CATG 2 cut(s) 195, 511
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 4 cut(s) 24, 61, 142, 220
LmnI GCTCC 3 cut(s) 293, 357, 537
LpnPI CCDG 5 cut(s) 5, 141, 289, 354, 435
Lsp1109I GCAGC 4 cut(s) 245, 259, 283, 287
LweI GCATC 1 cut(s) 537
MaeI CTAG 3 cut(s) 14, 102, 281
MaeIII GTNAC 3 cut(s) 38, 484, 617
MalI GATC 4 cut(s) 26, 63, 144, 222
MboI GATC 4 cut(s) 24, 61, 142, 220
MboII GAAGA 2 cut(s) 552, 555
MhlI GDGCHC 2 cut(s) 362, 534
MluCI AATT 3 cut(s) 45, 151, 392
MlyI GAGTC 2 cut(s) 109, 374
MnlI CCTC 9 cut(s) 103, 150, 167, 174, 262, 418, 429, 514, 594
MroXI GAANNNNTTC 2 cut(s) 79, 541
MseI TTAA 3 cut(s) 48, 86, 228
MwoI GCNNNNNNNGC 2 cut(s) 272, 281
NcoI CCATGG 1 cut(s) 191
NdeII GATC 4 cut(s) 24, 61, 142, 220
NlaIII CATG 2 cut(s) 195, 511
NlaIV GGNNCC 1 cut(s) 582
NmeAIII GCCGAG 2 cut(s) 228, 292
NmuCI GTSAC 3 cut(s) 38, 484, 617
PdmI GAANNNNTTC 2 cut(s) 79, 541
PfeI GAWTC 1 cut(s) 214
PkrI GCNGC 6 cut(s) 208, 235, 274, 277, 298, 526
PleI GAGTC 2 cut(s) 109, 373
PpsI GAGTC 2 cut(s) 109, 373
PpuMI RGGWCCY 1 cut(s) 581
PshAI GACNNNNGTC 1 cut(s) 615
PshBI ATTAAT 1 cut(s) 48
PsiI TTATAA 1 cut(s) 591
Psp124BI GAGCTC 2 cut(s) 362, 534
Psp5II RGGWCCY 1 cut(s) 581
PspN4I GGNNCC 1 cut(s) 582
PspPI GGNCC 1 cut(s) 581
PspPPI RGGWCCY 1 cut(s) 581
PstI CTGCAG 1 cut(s) 277
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
SacI GAGCTC 2 cut(s) 362, 534
SaqAI TTAA 3 cut(s) 48, 86, 228
SatI GCNGC 6 cut(s) 207, 234, 273, 276, 297, 525
Sau3AI GATC 4 cut(s) 24, 61, 142, 220
Sau96I GGNCC 1 cut(s) 581
ScaI AGTACT 1 cut(s) 132
SchI GAGTC 2 cut(s) 109, 374
SduI GDGCHC 2 cut(s) 362, 534
SfaNI GCATC 1 cut(s) 537
SfcI CTRYAG 2 cut(s) 169, 273
SinI GGWCC 1 cut(s) 581
SmlI CTYRAG 1 cut(s) 622
SmoI CTYRAG 1 cut(s) 622
Sse9I AATT 3 cut(s) 45, 151, 392
SsiI CCGC 4 cut(s) 184, 206, 209, 525
SspI AATATT 2 cut(s) 415, 433
SspMI CTAG 3 cut(s) 14, 102, 281
SstI GAGCTC 2 cut(s) 362, 534
StyI CCWWGG 1 cut(s) 191
TaaI ACNGT 1 cut(s) 99
TaqI TCGA 4 cut(s) 439, 462, 545, 572
TasI AATT 3 cut(s) 45, 151, 392
TatI WGTACW 1 cut(s) 130
TauI GCSGC 2 cut(s) 209, 527
TfiI GAWTC 1 cut(s) 214
Tru1I TTAA 3 cut(s) 48, 86, 228
Tru9I TTAA 3 cut(s) 48, 86, 228
TseFI GTSAC 3 cut(s) 38, 484, 617
TseI GCWGC 4 cut(s) 233, 272, 275, 296
Tsp45I GTSAC 3 cut(s) 38, 484, 617
VpaK11BI GGWCC 1 cut(s) 581
VspI ATTAAT 1 cut(s) 48
XapI RAATTY 1 cut(s) 151
XcmI CCANNNNNNNNNTGG 1 cut(s) 198
XmnI GAANNNNTTC 2 cut(s) 79, 541
XspI CTAG 3 cut(s) 14, 102, 281
ZrmI AGTACT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.