MD06G1130100.v1.1
ERF Family

Ethylene-responsive transcription factor ERF114-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012764
Physical Location & Seq
Reverse (-)
27370885 .. 27371948
1064 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1130100.v1.1.491

Sequence Viewer

Length: 369 bp
ATGGTTTCAGCTCTAGCTCAGGTGATTGGAAAAAATAGTGACCAAATTAATAACCCACTTGATCAAGTGCAAGGAATAAATCCATTAATCACCTCACAGTCCAGCCCTACAGAGACTCAATCTCAAACAGTACTTCAAGATCAAGGAAATTTGAGGAGACAACATTACAGAGGAGTGAGGCGGAGACCATCGGGAAAGTGGGCTGCTGAGATTCGTGACCCGGTGAAGGCAGCCCGAGTGTGGCTGGGCACTTTTGACACAGCTGAGGCTGCAGCACTAGCTTATGATAAAGCAGCACTCAAGTTCAAAGGAAGCAAAGCTAAGCTAAACTTCCCTGAGAGAGTTCAAGGGATCTCTGAGTCAGCCTAA

Protein Analysis

123

Amino Acids

13.36

Weight (kDa)

9.99

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 55 - 104 1.2e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012764)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07310 AT5G61890
fragaria_vesca FvH4_5g09520
malus_domestica MD06G1130100.v1.1 MD06G1130300.v1.1 MD14G1147100.v1.1
prunus_persica Prupe.5G141200_v2.0.a1
pyrus_communis pycom14g12170
rosa_chinensis RchiOBHm_Chr7g0188681
rosa_laevigata RLG00000004671
rosa_multiflora Rmu_sc0001576.1_g000027
rosa_roxburghii Rroxscaffold_3G00266140
rosa_rugosa Rorug06G0492200
rosa_samantha Rh7AG097100 Rh7BG098900 Rh7CG100700 Rh7DG099300
rosa_wichuraiana Rw7G008400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 181
AclWI GGATC 1 cut(s) 359
AcsI RAATTY 1 cut(s) 148
AfaI GTAC 1 cut(s) 132
AfiI CCNNNNNNNGG 2 cut(s) 226, 240
AgsI TTSAA 3 cut(s) 137, 307, 347
AluBI AGCT 6 cut(s) 11, 17, 263, 281, 320, 325
AluI AGCT 6 cut(s) 11, 17, 263, 281, 320, 325
Alw26I GTCTC 3 cut(s) 107, 151, 178
AlwI GGATC 1 cut(s) 359
Ama87I CYCGRG 1 cut(s) 234
ApeKI GCWGC 5 cut(s) 203, 230, 269, 272, 293
ApoI RAATTY 1 cut(s) 148
AseI ATTAAT 2 cut(s) 48, 86
AsuC2I CCSGG 1 cut(s) 221
AsuHPI GGTGA 3 cut(s) 34, 82, 235
AvaI CYCGRG 1 cut(s) 234
BaeGI GKGCMC 1 cut(s) 251
BbvCI CCTCAGC 1 cut(s) 264
BbvI GCAGC 5 cut(s) 190, 242, 256, 284, 305
BccI CCATC 1 cut(s) 196
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 1 cut(s) 221
BcoDI GTCTC 3 cut(s) 107, 151, 178
BfaI CTAG 2 cut(s) 14, 278
BfmI CTRYAG 2 cut(s) 108, 270
BisI GCNGC 5 cut(s) 204, 231, 270, 273, 294
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 5 cut(s) 205, 232, 271, 274, 295
BmcAI AGTACT 1 cut(s) 132
Bme1390I CCNGG 1 cut(s) 221
BmeT110I CYCGRG 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 221
Bpu10I CCTNAGC 2 cut(s) 18, 264
Bpu1102I GCTNAGC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 284
BpuMI CCSGG 1 cut(s) 221
BsaI GGTCTC 1 cut(s) 178
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 240
BseLI CCNNNNNNNGG 2 cut(s) 226, 240
BseMII CTCAG 5 cut(s) 32, 198, 255, 327, 348
BseRI GAGGAG 2 cut(s) 169, 186
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 5 cut(s) 190, 242, 256, 284, 305
BseYI CCCAGC 1 cut(s) 244
BsiHKCI CYCGRG 1 cut(s) 234
BsiSI CCGG 1 cut(s) 221
BslI CCNNNNNNNGG 2 cut(s) 226, 240
BsmAI GTCTC 3 cut(s) 107, 151, 178
Bso31I GGTCTC 1 cut(s) 178
BsoBI CYCGRG 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 3 cut(s) 61, 139, 351
Bsp1720I GCTNAGC 1 cut(s) 321
BspACI CCGC 1 cut(s) 181
BspCNI CTCAG 5 cut(s) 31, 199, 256, 328, 349
BspMAI CTGCAG 1 cut(s) 274
BspPI GGATC 1 cut(s) 359
BspTNI GGTCTC 1 cut(s) 178
BssMI GATC 3 cut(s) 61, 139, 351
Bst4CI ACNGT 2 cut(s) 99, 130
BstDEI CTNAG 6 cut(s) 18, 207, 264, 321, 336, 357
BstKTI GATC 3 cut(s) 64, 142, 354
BstMAI GTCTC 3 cut(s) 107, 151, 178
BstMBI GATC 3 cut(s) 61, 139, 351
BstMWI GCNNNNNNNGC 2 cut(s) 269, 278
BstSCI CCNGG 1 cut(s) 219
BstSFI CTRYAG 2 cut(s) 108, 270
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 5 cut(s) 190, 242, 256, 284, 305
BstX2I RGATCY 1 cut(s) 351
BstYI RGATCY 1 cut(s) 351
Csp6I GTAC 1 cut(s) 131
CviQI GTAC 1 cut(s) 131
DdeI CTNAG 6 cut(s) 18, 207, 264, 321, 336, 357
DpnI GATC 3 cut(s) 63, 141, 353
DpnII GATC 3 cut(s) 61, 139, 351
EciI GGCGGA 1 cut(s) 196
Eco31I GGTCTC 1 cut(s) 178
Eco88I CYCGRG 1 cut(s) 234
FaiI YATR 1 cut(s) 285
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 5 cut(s) 204, 231, 270, 273, 294
Fsp4HI GCNGC 5 cut(s) 204, 231, 270, 273, 294
FspBI CTAG 2 cut(s) 14, 278
GluI GCNGC 5 cut(s) 204, 231, 270, 273, 294
GsaI CCCAGC 1 cut(s) 248
HapII CCGG 1 cut(s) 221
HinfI GANTC 3 cut(s) 115, 211, 359
HpaII CCGG 1 cut(s) 221
HphI GGTGA 3 cut(s) 34, 82, 235
Hpy188I TCNGA 1 cut(s) 358
Hpy188III TCNNGA 3 cut(s) 137, 192, 215
HpyAV CCTTC 1 cut(s) 220
HpyCH4III ACNGT 2 cut(s) 99, 130
HpyCH4V TGCA 2 cut(s) 70, 272
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 278
HpyF3I CTNAG 6 cut(s) 18, 207, 264, 321, 336, 357
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 3 cut(s) 61, 139, 351
LpnPI CCDG 5 cut(s) 5, 115, 230, 234, 348
Lsp1109I GCAGC 5 cut(s) 190, 242, 256, 284, 305
MaeI CTAG 2 cut(s) 14, 278
MaeIII GTNAC 2 cut(s) 38, 215
MalI GATC 3 cut(s) 63, 141, 353
MboI GATC 3 cut(s) 61, 139, 351
MflI RGATCY 1 cut(s) 351
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 2 cut(s) 45, 148
MlyI GAGTC 2 cut(s) 109, 368
MnlI CCTC 5 cut(s) 103, 147, 164, 171, 259
MseI TTAA 2 cut(s) 48, 86
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 269, 278
NciI CCSGG 1 cut(s) 221
NdeII GATC 3 cut(s) 61, 139, 351
NmuCI GTSAC 2 cut(s) 38, 215
PfeI GAWTC 1 cut(s) 211
PkrI GCNGC 5 cut(s) 205, 232, 271, 274, 295
PleI GAGTC 2 cut(s) 109, 367
PpsI GAGTC 2 cut(s) 109, 367
PshBI ATTAAT 2 cut(s) 48, 86
PspFI CCCAGC 1 cut(s) 244
PstI CTGCAG 1 cut(s) 274
PsuI RGATCY 1 cut(s) 351
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
SaqAI TTAA 2 cut(s) 48, 86
SatI GCNGC 5 cut(s) 204, 231, 270, 273, 294
Sau3AI GATC 3 cut(s) 61, 139, 351
ScaI AGTACT 1 cut(s) 132
SchI GAGTC 2 cut(s) 109, 368
ScrFI CCNGG 1 cut(s) 221
SduI GDGCHC 1 cut(s) 251
SetI ASST 8 cut(s) 13, 19, 24, 95, 265, 283, 322, 327
SfcI CTRYAG 2 cut(s) 108, 270
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 2 cut(s) 45, 148
SsiI CCGC 1 cut(s) 181
SspMI CTAG 2 cut(s) 14, 278
StyD4I CCNGG 1 cut(s) 219
TaaI ACNGT 2 cut(s) 99, 130
TasI AATT 2 cut(s) 45, 148
TatI WGTACW 1 cut(s) 130
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 2 cut(s) 48, 86
Tru9I TTAA 2 cut(s) 48, 86
TseFI GTSAC 2 cut(s) 38, 215
TseI GCWGC 5 cut(s) 203, 230, 269, 272, 293
Tsp45I GTSAC 2 cut(s) 38, 215
VspI ATTAAT 2 cut(s) 48, 86
XapI RAATTY 1 cut(s) 148
XcmI CCANNNNNNNNNTGG 1 cut(s) 195
XspI CTAG 2 cut(s) 14, 278
ZrmI AGTACT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.