Rorug06G0492200

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012764
Physical Location & Seq
Forward (+)
61009349 .. 61012772
3424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0492200.1

Sequence Viewer

Length: 1161 bp
ATGCAATCAAGGGTTGTGCCATTAGAGGAAGGCAAGGACCCGGGAGTTAGTACTAGCAGGACGAACCAAACCAAGGCGTTGCCGATTCGGATGCTTCAGTTATTTGTGCTGTTTCTGGCTTTCTGTATCACTTTCTCAGTGATTAGTATATACACCATTCGGCATTTTGGGATTAGTAACGTGATGACTTCAGTCACGTCCTCTGTCCAGCCATGTTTTGAGGAACCAACTGGTTTGGATAGATGGATTAAGCCGCCATCGAATTTGATGCACACTATGAATGACGAAGAACTGTTGTGGAGGGCTTCTTTGTCGTCTAGGAGAAAGAACTATCCTTTTGAGAGAGTTCCGAAGATTGCGCTTATGTTCTTGACTAAGGGGCCTTTGCCTCTGGCACCAATTTGGGAGAGGTTTCTGAAGGGGCATGAAGGATTTTATTCAGTCTATGTTCATTCACTGCCATCATTTCAGCCTCATTTTCCACCTTCCTCGGTTTTTCATGGGAGACATATTCCTAGCCAGGTTGCTGAATGGGGAAGGATGAGTATGTGTGATGCTGAGAGGAGACTTCTTGCTAATGCATTGCTTGACATATCCAACGAATGGTTCATTCTTGTTTCTGAATCATGCATTCCTCTCTATAACTTCAGCGTCATTTACCAGTACATGAGGAAGTCCAAGTATAGCTACATGGGTGCATTTGATGACCACGGCCCGTATGGCAGAGGACGCTACAATGATAACATGGCTCCTGAAGTGAAGATTACCCAGTGGCGTAAGGGATCCCAGTGGTTTGAAGTTAACCGGAAGCTTGCCATCGCCATCGTTGAAGATACAACATTTTACCCAAAATTCAAAGAGTTCTGCAGACCAGCATGTTATGTTGATGAGCACTACTTCCCCACAATGCTAACCATTCAAGCAGGGAATATGCTAGCAAACAGAAGCATCACTTGGGTGGATTGGTCAAGGGGCGGACCTCACCCGGCCACCTTTGGCAGAGGAGATATCACCGAGGAATTCATGAAGCGGATGCTCTCAAGTCATCGTTGCACTTACAACAACCGAAACTCTACAATGTGCTTTCTATTTGCAAGAAAATTTGCTCCCAGTGCCTTGGAACCTCTCTTACAGTTAGCACCAAAGTTTTTGGGATTCTGA

Protein Analysis

386

Amino Acids

44.65

Weight (kDa)

9.25

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 119 - 345 2.3e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012764)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07310 AT5G61890
fragaria_vesca FvH4_5g09520
malus_domestica MD06G1130100.v1.1 MD06G1130300.v1.1 MD14G1147100.v1.1
prunus_persica Prupe.5G141200_v2.0.a1
pyrus_communis pycom14g12170
rosa_chinensis RchiOBHm_Chr7g0188681
rosa_laevigata RLG00000004671
rosa_multiflora Rmu_sc0001576.1_g000027
rosa_roxburghii Rroxscaffold_3G00266140
rosa_rugosa Rorug06G0492200
rosa_samantha Rh7AG097100 Rh7BG098900 Rh7CG100700 Rh7DG099300
rosa_wichuraiana Rw7G008400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 394
AccB7I CCANNNNNTGG 1 cut(s) 603
AciI CCGC 3 cut(s) 254, 975, 1030
AclWI GGATC 2 cut(s) 777, 790
AcoI YGGCCR 1 cut(s) 987
AcsI RAATTY 4 cut(s) 262, 851, 1019, 1100
AcuI CTGAAG 5 cut(s) 80, 174, 437, 631, 774
AfaI GTAC 2 cut(s) 52, 665
AfiI CCNNNNNNNGG 2 cut(s) 73, 603
AgsI TTSAA 4 cut(s) 797, 830, 856, 920
AjiI CACGTC 1 cut(s) 198
AjnI CCWGG 1 cut(s) 519
AjuI GAANNNNNNNTTGG 4 cut(s) 590, 622, 937, 969
AleI CACNNNNGTG 1 cut(s) 956
AluBI AGCT 2 cut(s) 687, 811
AluI AGCT 2 cut(s) 687, 811
Alw21I GWGCWC 1 cut(s) 894
Alw26I GTCTC 2 cut(s) 499, 559
AlwI GGATC 2 cut(s) 777, 790
Ama87I CYCGRG 1 cut(s) 40
AoxI GGCC 3 cut(s) 380, 712, 987
ApoI RAATTY 4 cut(s) 262, 851, 1019, 1100
AspLEI GCGC 1 cut(s) 361
AspS9I GGNCC 4 cut(s) 37, 380, 713, 977
AsuC2I CCSGG 3 cut(s) 41, 42, 986
AsuHPI GGTGA 2 cut(s) 974, 1003
AsuNHI GCTAGC 1 cut(s) 934
AvaI CYCGRG 1 cut(s) 40
AvaII GGWCC 2 cut(s) 37, 977
BamHI GGATCC 1 cut(s) 782
BanI GGYRCC 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 894
BccI CCATC 5 cut(s) 237, 265, 469, 824, 830
BceAI ACGGC 1 cut(s) 727
BcgI CGANNNNNNTGC 4 cut(s) 73, 107, 250, 284
BciT130I CCWGG 1 cut(s) 521
BcnI CCSGG 3 cut(s) 41, 42, 986
BcoDI GTCTC 2 cut(s) 499, 559
BfaI CTAG 4 cut(s) 54, 318, 516, 935
BfmI CTRYAG 1 cut(s) 865
BglI GCCNNNNNGGC 1 cut(s) 720
BisI GCNGC 1 cut(s) 254
BlsI GCNGC 1 cut(s) 255
BmcAI AGTACT 1 cut(s) 52
Bme1390I CCNGG 4 cut(s) 41, 42, 521, 986
Bme18I GGWCC 2 cut(s) 37, 977
BmeT110I CYCGRG 1 cut(s) 40
BmgBI CACGTC 1 cut(s) 198
BmgT120I GGNCC 4 cut(s) 37, 380, 713, 977
BmiI GGNNCC 7 cut(s) 39, 225, 381, 396, 750, 784, 1122
BmrFI CCNGG 4 cut(s) 41, 42, 521, 986
BmrI ACTGGG 3 cut(s) 763, 781, 1104
BmsI GCATC 5 cut(s) 81, 258, 544, 957, 1023
BmtI GCTAGC 1 cut(s) 938
BmuI ACTGGG 3 cut(s) 763, 781, 1104
BoxI GACNNNNGTC 1 cut(s) 191
BpuEI CTTGAG 1 cut(s) 1024
BpuMI CCSGG 3 cut(s) 41, 42, 986
BsaJI CCNNGG 6 cut(s) 40, 72, 489, 709, 1014, 1116
BsaWI WCCGGW 1 cut(s) 804
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 603
Bse1I ACTGG 5 cut(s) 235, 661, 769, 787, 1110
Bse3DI GCAATG 1 cut(s) 581
BseBI CCWGG 1 cut(s) 521
BseDI CCNNGG 6 cut(s) 40, 72, 489, 709, 1014, 1116
BseGI GGATG 3 cut(s) 96, 546, 1038
BseLI CCNNNNNNNGG 2 cut(s) 73, 603
BseMI GCAATG 1 cut(s) 581
BseMII CTCAG 2 cut(s) 150, 549
BseNI ACTGG 5 cut(s) 235, 661, 769, 787, 1110
BseRI GAGGAG 2 cut(s) 577, 1017
BshFI GGCC 3 cut(s) 382, 714, 989
BshNI GGYRCC 1 cut(s) 394
BsiHKAI GWGCWC 1 cut(s) 894
BsiHKCI CYCGRG 1 cut(s) 40
BsiSI CCGG 3 cut(s) 41, 805, 986
BslI CCNNNNNNNGG 2 cut(s) 73, 603
BsmAI GTCTC 2 cut(s) 499, 559
BsmI GAATGC 1 cut(s) 630
BsnI GGCC 3 cut(s) 382, 714, 989
BsoBI CYCGRG 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 894
Bsp143I GATC 1 cut(s) 782
BspACI CCGC 3 cut(s) 254, 975, 1030
BspANI GGCC 3 cut(s) 382, 714, 989
BspCNI CTCAG 2 cut(s) 149, 550
BspHI TCATGA 1 cut(s) 1023
BspLI GGNNCC 7 cut(s) 39, 225, 381, 396, 750, 784, 1122
BspMAI CTGCAG 1 cut(s) 869
BspOI GCTAGC 1 cut(s) 938
BspPI GGATC 2 cut(s) 777, 790
BspT107I GGYRCC 1 cut(s) 394
BsrDI GCAATG 1 cut(s) 581
BsrI ACTGG 5 cut(s) 235, 661, 769, 787, 1110
BssECI CCNNGG 6 cut(s) 40, 72, 489, 709, 1014, 1116
BssMI GATC 1 cut(s) 782
BssT1I CCWWGG 2 cut(s) 72, 1116
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 2 cut(s) 294, 1134
BstC8I GCNNGC 2 cut(s) 813, 936
BstDEI CTNAG 3 cut(s) 136, 375, 558
BstDSI CCRYGG 1 cut(s) 709
BstF5I GGATG 3 cut(s) 96, 546, 1038
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 1 cut(s) 785
BstMAI GTCTC 2 cut(s) 499, 559
BstMBI GATC 1 cut(s) 782
BstMWI GCNNNNNNNGC 3 cut(s) 720, 729, 1112
BstNI CCWGG 1 cut(s) 521
BstNSI RCATGY 1 cut(s) 879
BstPAI GACNNNNGTC 1 cut(s) 191
BstSCI CCNGG 4 cut(s) 39, 40, 519, 984
BstSFI CTRYAG 1 cut(s) 865
BstX2I RGATCY 1 cut(s) 782
BstXI CCANNNNNNTGG 1 cut(s) 1117
BstYI RGATCY 1 cut(s) 782
BsuRI GGCC 3 cut(s) 382, 714, 989
BtgI CCRYGG 1 cut(s) 709
BtgZI GCGATG 1 cut(s) 802
BtrI CACGTC 1 cut(s) 198
BtsCI GGATG 3 cut(s) 96, 546, 1038
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 5 cut(s) 144, 455, 776, 794, 1117
Cac8I GCNNGC 2 cut(s) 813, 936
CciI TCATGA 1 cut(s) 1023
CfoI GCGC 1 cut(s) 361
Cfr13I GGNCC 4 cut(s) 37, 380, 713, 977
Cfr9I CCCGGG 1 cut(s) 40
CseI GACGC 2 cut(s) 640, 738
Csp6I GTAC 2 cut(s) 51, 664
CviAII CATG 9 cut(s) 213, 425, 500, 627, 667, 691, 745, 876, 1024
CviQI GTAC 2 cut(s) 51, 664
DdeI CTNAG 3 cut(s) 136, 375, 558
DpnI GATC 1 cut(s) 784
DpnII GATC 1 cut(s) 782
EaeI YGGCCR 1 cut(s) 987
EciI GGCGGA 1 cut(s) 990
Eco130I CCWWGG 2 cut(s) 72, 1116
Eco32I GATATC 1 cut(s) 1009
Eco47I GGWCC 2 cut(s) 37, 977
Eco57I CTGAAG 5 cut(s) 80, 174, 437, 631, 774
Eco88I CYCGRG 1 cut(s) 40
EcoO109I RGGNCCY 2 cut(s) 37, 380
EcoRI GAATTC 1 cut(s) 1019
EcoRII CCWGG 1 cut(s) 519
EcoRV GATATC 1 cut(s) 1009
EcoT14I CCWWGG 2 cut(s) 72, 1116
EcoT22I ATGCAT 2 cut(s) 583, 632
ErhI CCWWGG 2 cut(s) 72, 1116
FaeI CATG 9 cut(s) 216, 428, 503, 630, 670, 694, 748, 879, 1027
FalI AAGNNNNNCTT 2 cut(s) 937, 969
FatI CATG 9 cut(s) 212, 424, 499, 626, 666, 690, 744, 875, 1023
Fnu4HI GCNGC 1 cut(s) 254
FokI GGATG 3 cut(s) 103, 553, 1045
Fsp4HI GCNGC 1 cut(s) 254
FspBI CTAG 4 cut(s) 54, 318, 516, 935
GlaI GCGC 1 cut(s) 360
GluI GCNGC 1 cut(s) 254
HaeIII GGCC 3 cut(s) 382, 714, 989
HapII CCGG 3 cut(s) 41, 805, 986
HgaI GACGC 2 cut(s) 640, 738
HhaI GCGC 1 cut(s) 361
Hin1II CATG 9 cut(s) 216, 428, 503, 630, 670, 694, 748, 879, 1027
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 3 cut(s) 85, 623, 1155
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 3 cut(s) 41, 805, 986
HphI GGTGA 2 cut(s) 974, 1003
Hpy166II GTNNAC 1 cut(s) 802
Hpy188I TCNGA 5 cut(s) 90, 351, 417, 622, 1160
Hpy188III TCNNGA 3 cut(s) 370, 752, 1024
Hpy8I GTNNAC 1 cut(s) 802
HpyAV CCTTC 5 cut(s) 23, 412, 422, 495, 531
HpyCH4III ACNGT 2 cut(s) 294, 1134
HpyCH4IV ACGT 2 cut(s) 180, 197
HpyCH4V TGCA 8 cut(s) 4, 271, 581, 630, 698, 867, 1053, 1094
HpyF10VI GCNNNNNNNGC 3 cut(s) 720, 729, 1112
HpyF3I CTNAG 3 cut(s) 136, 375, 558
HpySE526I ACGT 2 cut(s) 180, 197
Hsp92II CATG 9 cut(s) 216, 428, 503, 630, 670, 694, 748, 879, 1027
HspAI GCGC 1 cut(s) 359
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 1 cut(s) 782
LmnI GCTCC 2 cut(s) 754, 1111
LweI GCATC 5 cut(s) 81, 258, 544, 957, 1023
MaeI CTAG 4 cut(s) 54, 318, 516, 935
MaeII ACGT 2 cut(s) 180, 197
MaeIII GTNAC 2 cut(s) 176, 193
MalI GATC 1 cut(s) 784
MboI GATC 1 cut(s) 782
MboII GAAGA 4 cut(s) 299, 364, 772, 842
MflI RGATCY 1 cut(s) 782
MhlI GDGCHC 1 cut(s) 894
MluCI AATT 5 cut(s) 262, 399, 851, 1019, 1100
MmeI TCCRAC 1 cut(s) 621
Mph1103I ATGCAT 2 cut(s) 583, 632
MseI TTAA 2 cut(s) 249, 801
MslI CAYNNNNRTG 1 cut(s) 956
MspI CCGG 3 cut(s) 41, 805, 986
MspR9I CCNGG 4 cut(s) 41, 42, 521, 986
Mva1269I GAATGC 1 cut(s) 630
MvaI CCWGG 1 cut(s) 521
MwoI GCNNNNNNNGC 3 cut(s) 720, 729, 1112
NciI CCSGG 3 cut(s) 41, 42, 986
NdeII GATC 1 cut(s) 782
NheI GCTAGC 1 cut(s) 934
NlaIII CATG 9 cut(s) 216, 428, 503, 630, 670, 694, 748, 879, 1027
NlaIV GGNNCC 7 cut(s) 39, 225, 381, 396, 750, 784, 1122
NmuCI GTSAC 1 cut(s) 193
NsiI ATGCAT 2 cut(s) 583, 632
NspI RCATGY 1 cut(s) 879
OliI CACNNNNGTG 1 cut(s) 956
PagI TCATGA 1 cut(s) 1023
PctI GAATGC 1 cut(s) 630
PfeI GAWTC 3 cut(s) 85, 623, 1155
PflMI CCANNNNNTGG 1 cut(s) 603
PkrI GCNGC 1 cut(s) 255
PpuMI RGGWCCY 1 cut(s) 37
PshAI GACNNNNGTC 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 519
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 7 cut(s) 39, 225, 381, 396, 750, 784, 1122
PspPI GGNCC 4 cut(s) 37, 380, 713, 977
PspPPI RGGWCCY 1 cut(s) 37
PsrI GAACNNNNNNTAC 2 cut(s) 1113, 1145
PstI CTGCAG 1 cut(s) 869
PsuI RGATCY 1 cut(s) 782
RsaI GTAC 2 cut(s) 52, 665
RsaNI GTAC 2 cut(s) 51, 664
RseI CAYNNNNRTG 1 cut(s) 956
SaqAI TTAA 2 cut(s) 249, 801
SatI GCNGC 1 cut(s) 254
Sau3AI GATC 1 cut(s) 782
Sau96I GGNCC 4 cut(s) 37, 380, 713, 977
ScaI AGTACT 1 cut(s) 52
ScrFI CCNGG 4 cut(s) 41, 42, 521, 986
SduI GDGCHC 1 cut(s) 894
SfaNI GCATC 5 cut(s) 81, 258, 544, 957, 1023
SfcI CTRYAG 1 cut(s) 865
SinI GGWCC 2 cut(s) 37, 977
SmaI CCCGGG 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 956
SmlI CTYRAG 1 cut(s) 1039
SmoI CTYRAG 1 cut(s) 1039
Sse9I AATT 5 cut(s) 262, 399, 851, 1019, 1100
SsiI CCGC 3 cut(s) 254, 975, 1030
SspMI CTAG 4 cut(s) 54, 318, 516, 935
StyD4I CCNGG 4 cut(s) 39, 40, 519, 984
StyI CCWWGG 2 cut(s) 72, 1116
TaaI ACNGT 2 cut(s) 294, 1134
TaiI ACGT 2 cut(s) 183, 200
TaqI TCGA 1 cut(s) 260
TasI AATT 5 cut(s) 262, 399, 851, 1019, 1100
TatI WGTACW 2 cut(s) 50, 663
TauI GCSGC 1 cut(s) 256
TfiI GAWTC 3 cut(s) 85, 623, 1155
Tru1I TTAA 2 cut(s) 249, 801
Tru9I TTAA 2 cut(s) 249, 801
TscAI CASTG 5 cut(s) 144, 462, 776, 794, 1117
TseFI GTSAC 1 cut(s) 193
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 7 cut(s) 293, 440, 441, 488, 598, 1012, 1040
TspMI CCCGGG 1 cut(s) 40
TspRI CASTG 5 cut(s) 144, 462, 776, 794, 1117
Van91I CCANNNNNTGG 1 cut(s) 603
VpaK11BI GGWCC 2 cut(s) 37, 977
XapI RAATTY 4 cut(s) 262, 851, 1019, 1100
XceI RCATGY 1 cut(s) 879
XcmI CCANNNNNNNNNTGG 1 cut(s) 716
XmaI CCCGGG 1 cut(s) 40
XspI CTAG 4 cut(s) 54, 318, 516, 935
ZrmI AGTACT 1 cut(s) 52
Zsp2I ATGCAT 2 cut(s) 583, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.