Rh7CG100700

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0012764
Physical Location & Seq
Reverse (-)
7532592 .. 7534228
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG100700.1

Sequence Viewer

Length: 786 bp
ATGGTATCAGCTCTATCTCAGGTGATTGCAAACACTGATGAACAAAATCATCAAGTTCATGGTATCCCACTAATAATTTCAGCAGATAATCATGTTGAGCAATCTAAACCTGCAGTAGTTCTTCAAGCTCAAGGGAATATGAGACAACACTATAGAGGAGTGAGGCGGAGGCCATGGGGGAAGTGGGCAGCCGAGATTCGTGATCCGCAGAAGGCAGCTCGAGTGTGGCTCGGGACTTTCGAATCAGCAGAGGCTGCAGCGCTTGCTTACGACGAAGCAGCTCTCAGGTTCAAAGGAAGCAAGGCTAAGCTTAACTTCCCCGAAAGAGTTCAAGGGATCAGCCCTAGTACTTCTAGTGGTCGCTACCTTGCAATAAGTAGTACCGATGATGATCAGCGAGTAGACAATTCAGCTCCTCCAGCTGCGCCGATTTGCCAACCAACAACTTATAGTATTCCCAATGATATTAATTTTGATTATGATGCACAACATCTTGGGAGTAGTACTACAAGTAACAGAAATGTTGATTTCGGTGTATCATCGTCTCAAAACTACGGACAAGAAAGAATTATGCCGGTGATTAATGTACCAACGACTTCTTCTTCTACATCCTCGGCCTCTTCCATGCCATCTCATCATCAACAAGTACAACTACAGCAGCAAGAAGATCAGCAGCAACTCTTAAGGTTTCCGATGATGCCTTTTGGCGGTTCATCTTCGAGCTCATCAGATCCTCCTGCTAAGTACAGAAGAGACTCCTCTGGGAGTGGTGACTTTAGAAGATGA

Protein Analysis

261

Amino Acids

28.62

Weight (kDa)

7.07

Isoelectric Point (pI)

60.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 50 - 99 7.5e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012764)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07310 AT5G61890
fragaria_vesca FvH4_5g09520
malus_domestica MD06G1130100.v1.1 MD06G1130300.v1.1 MD14G1147100.v1.1
prunus_persica Prupe.5G141200_v2.0.a1
pyrus_communis pycom14g12170
rosa_chinensis RchiOBHm_Chr7g0188681
rosa_laevigata RLG00000004671
rosa_multiflora Rmu_sc0001576.1_g000027
rosa_roxburghii Rroxscaffold_3G00266140
rosa_rugosa Rorug06G0492200
rosa_samantha Rh7AG097100 Rh7BG098900 Rh7CG100700 Rh7DG099300
rosa_wichuraiana Rw7G008400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 118
AccI GTMKAC 1 cut(s) 402
AciI CCGC 3 cut(s) 166, 206, 708
AclWI GGATC 3 cut(s) 197, 344, 725
AfaI GTAC 6 cut(s) 349, 382, 505, 588, 648, 746
AfeI AGCGCT 1 cut(s) 261
AfiI CCNNNNNNNGG 1 cut(s) 707
AflII CTTAAG 1 cut(s) 682
AgsI TTSAA 3 cut(s) 125, 292, 332
AjuI GAANNNNNNNTTGG 2 cut(s) 583, 615
AluBI AGCT 8 cut(s) 11, 128, 218, 281, 310, 413, 422, 723
AluI AGCT 8 cut(s) 11, 128, 218, 281, 310, 413, 422, 723
Alw21I GWGCWC 1 cut(s) 725
Alw26I GTCTC 3 cut(s) 136, 549, 747
AlwI GGATC 3 cut(s) 197, 344, 725
AlwNI CAGNNNCTG 1 cut(s) 254
Ama87I CYCGRG 2 cut(s) 219, 230
Aor51HI AGCGCT 1 cut(s) 261
AoxI GGCC 2 cut(s) 170, 615
ApeKI GCWGC 8 cut(s) 188, 215, 254, 257, 278, 422, 658, 673
AseI ATTAAT 2 cut(s) 468, 582
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 2 cut(s) 262, 427
AsuHPI GGTGA 3 cut(s) 34, 589, 782
AsuII TTCGAA 1 cut(s) 240
AvaI CYCGRG 2 cut(s) 219, 230
BanII GRGCYC 1 cut(s) 725
Bbv12I GWGCWC 1 cut(s) 725
BbvI GCAGC 8 cut(s) 200, 227, 241, 269, 290, 409, 670, 685
BccI CCATC 1 cut(s) 637
BciVI GTATCC 1 cut(s) 74
BclI TGATCA 1 cut(s) 391
BcoDI GTCTC 3 cut(s) 136, 549, 747
BfaI CTAG 2 cut(s) 345, 354
BfmI CTRYAG 4 cut(s) 111, 151, 255, 653
BfoI RGCGCY 1 cut(s) 263
BfrI CTTAAG 1 cut(s) 682
BfuAI ACCTGC 1 cut(s) 118
BfuI GTATCC 1 cut(s) 74
BisI GCNGC 8 cut(s) 189, 216, 255, 258, 279, 423, 659, 674
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 8 cut(s) 190, 217, 256, 259, 280, 424, 660, 675
BmcAI AGTACT 2 cut(s) 349, 505
BmeT110I CYCGRG 2 cut(s) 219, 230
BmsI GCATC 2 cut(s) 472, 687
BplI GAGNNNNNCTC 2 cut(s) 213, 245
BpmI CTGGAG 1 cut(s) 402
Bpu1102I GCTNAGC 1 cut(s) 306
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 390
BsaJI CCNNGG 2 cut(s) 173, 612
Bsc4I CCNNNNNNNGG 1 cut(s) 707
Bse118I RCCGGY 1 cut(s) 574
Bse8I GATNNNNATC 1 cut(s) 390
BseDI CCNNGG 2 cut(s) 173, 612
BseGI GGATG 1 cut(s) 608
BseJI GATNNNNATC 1 cut(s) 390
BseLI CCNNNNNNNGG 1 cut(s) 707
BseMII CTCAG 2 cut(s) 32, 298
BseRI GAGGAG 3 cut(s) 171, 405, 748
BseXI GCAGC 8 cut(s) 200, 227, 241, 269, 290, 409, 670, 685
BshFI GGCC 2 cut(s) 172, 617
BsiHKAI GWGCWC 1 cut(s) 725
BsiHKCI CYCGRG 2 cut(s) 219, 230
BsiSI CCGG 1 cut(s) 575
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 707
BsmAI GTCTC 3 cut(s) 136, 549, 747
BsmBI CGTCTC 1 cut(s) 549
BsmFI GGGAC 1 cut(s) 247
BsnI GGCC 2 cut(s) 172, 617
BsoBI CYCGRG 2 cut(s) 219, 230
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 725
Bsp143I GATC 5 cut(s) 202, 336, 391, 667, 730
Bsp1720I GCTNAGC 1 cut(s) 306
Bsp19I CCATGG 1 cut(s) 173
BspACI CCGC 3 cut(s) 166, 206, 708
BspANI GGCC 2 cut(s) 172, 617
BspCNI CTCAG 2 cut(s) 31, 297
BspMAI CTGCAG 2 cut(s) 115, 259
BspMI ACCTGC 1 cut(s) 118
BspPI GGATC 3 cut(s) 197, 344, 725
BspT104I TTCGAA 1 cut(s) 240
BspTI CTTAAG 1 cut(s) 682
BsrFI RCCGGY 1 cut(s) 574
BssAI RCCGGY 1 cut(s) 574
BssECI CCNNGG 2 cut(s) 173, 612
BssMI GATC 5 cut(s) 202, 336, 391, 667, 730
BssT1I CCWWGG 1 cut(s) 173
Bst6I CTCTTC 2 cut(s) 625, 745
BstAFI CTTAAG 1 cut(s) 682
BstAPI GCANNNNNTGC 2 cut(s) 254, 263
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 4 cut(s) 18, 284, 306, 741
BstDSI CCRYGG 1 cut(s) 173
BstF5I GGATG 1 cut(s) 608
BstH2I RGCGCY 1 cut(s) 263
BstHHI GCGC 2 cut(s) 262, 427
BstKTI GATC 5 cut(s) 205, 339, 394, 670, 733
BstMAI GTCTC 3 cut(s) 136, 549, 747
BstMBI GATC 5 cut(s) 202, 336, 391, 667, 730
BstMWI GCNNNNNNNGC 3 cut(s) 254, 263, 419
BstSFI CTRYAG 4 cut(s) 111, 151, 255, 653
BstV1I GCAGC 8 cut(s) 200, 227, 241, 269, 290, 409, 670, 685
BstX2I RGATCY 1 cut(s) 730
BstYI RGATCY 1 cut(s) 730
BsuI GTATCC 1 cut(s) 74
BsuRI GGCC 2 cut(s) 172, 617
BtgI CCRYGG 1 cut(s) 173
BtsCI GGATG 1 cut(s) 608
BtsIMutI CAGTG 1 cut(s) 33
BveI ACCTGC 1 cut(s) 118
Cac8I GCNNGC 1 cut(s) 264
CaiI CAGNNNCTG 1 cut(s) 254
CfoI GCGC 2 cut(s) 262, 427
Cfr10I RCCGGY 1 cut(s) 574
Csp6I GTAC 6 cut(s) 348, 381, 504, 587, 647, 745
CviAII CATG 4 cut(s) 59, 92, 174, 625
CviQI GTAC 6 cut(s) 348, 381, 504, 587, 647, 745
DdeI CTNAG 4 cut(s) 18, 284, 306, 741
DpnI GATC 5 cut(s) 204, 338, 393, 669, 732
DpnII GATC 5 cut(s) 202, 336, 391, 667, 730
Eam1104I CTCTTC 2 cut(s) 625, 745
EarI CTCTTC 2 cut(s) 625, 745
EciI GGCGGA 1 cut(s) 181
Ecl136II GAGCTC 1 cut(s) 723
Eco130I CCWWGG 1 cut(s) 173
Eco24I GRGCYC 1 cut(s) 725
Eco47III AGCGCT 1 cut(s) 261
Eco53kI GAGCTC 1 cut(s) 723
Eco88I CYCGRG 2 cut(s) 219, 230
EcoICRI GAGCTC 1 cut(s) 723
EcoT14I CCWWGG 1 cut(s) 173
EcoT38I GRGCYC 1 cut(s) 725
ErhI CCWWGG 1 cut(s) 173
Esp3I CGTCTC 1 cut(s) 549
FaeI CATG 4 cut(s) 62, 95, 177, 628
FaiI YATR 9 cut(s) 60, 93, 140, 153, 175, 450, 480, 572, 626
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 1 cut(s) 247
FatI CATG 4 cut(s) 58, 91, 173, 624
FbaI TGATCA 1 cut(s) 391
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 8 cut(s) 189, 216, 255, 258, 279, 423, 659, 674
FokI GGATG 1 cut(s) 595
FriOI GRGCYC 1 cut(s) 725
Fsp4HI GCNGC 8 cut(s) 189, 216, 255, 258, 279, 423, 659, 674
FspBI CTAG 2 cut(s) 345, 354
GlaI GCGC 2 cut(s) 261, 426
GluI GCNGC 8 cut(s) 189, 216, 255, 258, 279, 423, 659, 674
GsuI CTGGAG 1 cut(s) 402
HaeII RGCGCY 1 cut(s) 263
HaeIII GGCC 2 cut(s) 172, 617
HapII CCGG 1 cut(s) 575
HhaI GCGC 2 cut(s) 262, 427
Hin1II CATG 4 cut(s) 62, 95, 177, 628
Hin6I GCGC 2 cut(s) 260, 425
HinP1I GCGC 2 cut(s) 260, 425
HindIII AAGCTT 1 cut(s) 308
HinfI GANTC 3 cut(s) 196, 242, 755
HpaII CCGG 1 cut(s) 575
HphI GGTGA 3 cut(s) 34, 589, 782
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 2 cut(s) 693, 730
Hpy188III TCNNGA 2 cut(s) 200, 232
Hpy8I GTNNAC 1 cut(s) 403
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 1 cut(s) 205
HpyCH4V TGCA 5 cut(s) 29, 113, 257, 371, 485
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 263, 419
HpyF3I CTNAG 4 cut(s) 18, 284, 306, 741
Hsp92II CATG 4 cut(s) 62, 95, 177, 628
HspAI GCGC 2 cut(s) 260, 425
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 5 cut(s) 202, 336, 391, 667, 730
LmnI GCTCC 1 cut(s) 418
LpnPI CCDG 7 cut(s) 5, 123, 271, 432, 588, 747, 750
Lsp1109I GCAGC 8 cut(s) 200, 227, 241, 269, 290, 409, 670, 685
LweI GCATC 2 cut(s) 472, 687
MaeI CTAG 2 cut(s) 345, 354
MaeIII GTNAC 2 cut(s) 512, 770
MalI GATC 5 cut(s) 204, 338, 393, 669, 732
MboI GATC 5 cut(s) 202, 336, 391, 667, 730
MboII GAAGA 7 cut(s) 113, 591, 594, 612, 677, 708, 762
MflI RGATCY 1 cut(s) 730
MhlI GDGCHC 1 cut(s) 725
MluCI AATT 4 cut(s) 75, 406, 469, 567
MlyI GAGTC 1 cut(s) 749
MnlI CCTC 9 cut(s) 149, 156, 162, 244, 426, 622, 628, 744, 769
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 4 cut(s) 312, 468, 582, 683
MspA1I CMGCKG 1 cut(s) 422
MspCI CTTAAG 1 cut(s) 682
MspI CCGG 1 cut(s) 575
MwoI GCNNNNNNNGC 3 cut(s) 254, 263, 419
NcoI CCATGG 1 cut(s) 173
NdeII GATC 5 cut(s) 202, 336, 391, 667, 730
NlaIII CATG 4 cut(s) 62, 95, 177, 628
NmeAIII GCCGAG 2 cut(s) 217, 593
NmuCI GTSAC 1 cut(s) 770
NspV TTCGAA 1 cut(s) 240
PaeR7I CTCGAG 1 cut(s) 219
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 2 cut(s) 196, 242
PkrI GCNGC 8 cut(s) 190, 217, 256, 259, 280, 424, 660, 675
PleI GAGTC 1 cut(s) 749
PpsI GAGTC 1 cut(s) 749
PshBI ATTAAT 2 cut(s) 468, 582
Psp124BI GAGCTC 1 cut(s) 725
PspXI VCTCGAGB 1 cut(s) 219
PstI CTGCAG 2 cut(s) 115, 259
PstNI CAGNNNCTG 1 cut(s) 254
PsuI RGATCY 1 cut(s) 730
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 6 cut(s) 349, 382, 505, 588, 648, 746
RsaNI GTAC 6 cut(s) 348, 381, 504, 587, 647, 745
SacI GAGCTC 1 cut(s) 725
SaqAI TTAA 4 cut(s) 312, 468, 582, 683
SatI GCNGC 8 cut(s) 189, 216, 255, 258, 279, 423, 659, 674
Sau3AI GATC 5 cut(s) 202, 336, 391, 667, 730
ScaI AGTACT 2 cut(s) 349, 505
SchI GAGTC 1 cut(s) 749
SduI GDGCHC 1 cut(s) 725
SfaNI GCATC 2 cut(s) 472, 687
SfcI CTRYAG 4 cut(s) 111, 151, 255, 653
Sfr274I CTCGAG 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 240
SlaI CTCGAG 1 cut(s) 219
SmlI CTYRAG 3 cut(s) 129, 219, 682
SmoI CTYRAG 3 cut(s) 129, 219, 682
Sse9I AATT 4 cut(s) 75, 406, 469, 567
SsiI CCGC 3 cut(s) 166, 206, 708
SspMI CTAG 2 cut(s) 345, 354
SstI GAGCTC 1 cut(s) 725
StyI CCWWGG 1 cut(s) 173
TaqI TCGA 3 cut(s) 220, 240, 719
TasI AATT 4 cut(s) 75, 406, 469, 567
TatI WGTACW 4 cut(s) 347, 503, 646, 744
TfiI GAWTC 2 cut(s) 196, 242
Tru1I TTAA 4 cut(s) 312, 468, 582, 683
Tru9I TTAA 4 cut(s) 312, 468, 582, 683
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 1 cut(s) 770
TseI GCWGC 8 cut(s) 188, 215, 254, 257, 278, 422, 658, 673
Tsp45I GTSAC 1 cut(s) 770
TspDTI ATGAA 3 cut(s) 47, 54, 702
TspGWI ACGGA 1 cut(s) 570
TspRI CASTG 1 cut(s) 40
Vha464I CTTAAG 1 cut(s) 682
VspI ATTAAT 2 cut(s) 468, 582
XcmI CCANNNNNNNNNTGG 1 cut(s) 180
XhoI CTCGAG 1 cut(s) 219
XmiI GTMKAC 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 2 cut(s) 345, 354
ZrmI AGTACT 2 cut(s) 349, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.