RLG00000004671

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012764
Physical Location & Seq
Forward (+)
61313890 .. 61315371
1482 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004671

Sequence Viewer

Length: 951 bp
ATGTACCCTAAACCGTCCCACTGGTTTCTTGAAGAATCCTTGGATCGGAAACATGGGAAGAGGCCACTTCCTTCGAATGAATCGGAGAAGAAAGAGGAGGAGGTGGAGTATCATCGAAATATCTTCCCGGTTTACTCAAGTAGATCTCAACAAGATAATTCTGCCATGGTATCCGCTCTATCTCAGGTGATTGCAAACACTCATGAACAAAATCATCAAGTTCATGGTATCCCTCTAATAATTTCAGCAGATAATCATGTTGAGCAATCTAAACCTGCAGTAGTTCTTCAAGCTCAAGGGAATATGAGACAACACTATAGGGGAGTGAGGCGGAGGCCATGGGGGAAGTGGGCAGCCGAGATTCGTGATCCACAGAAGGCAGCAAGAGTGTGGCTCGGGACTTTCGAATCAGCAGAGGCTGCAGCACTTGCTTACGATGAAGCAGCTCTCAGGTTCAAAGGAAGCAAGGCTAAGCTTAACTTCCCCGAAAGAGTTCAAGGGATCAGCCCTAGTACTTCTAGTGGTCGCTACCTTGCAATAAGTAGTACCGATGATGATCAGCGAGTAGACAATTCAGCTCCTCCAGCTGCGCCGATTTTTCAACCAACAACCTATAGTATTCCAAATGATATTAATTTTGTTTATGATGCACAACATCTTGGGAGTAGTAGTACAAGTAACAGAAATGTTAATTTCGGTGTATCATCATCTCAAAACTACGGACAAGAAAGAATTATGCCGGAGGTTAATGTACCAACGACTTCTTCTTCTACATCCTCGGCCTCTTCCATGCCATCTCATCATCAACAAGTACGACTACAGCAGCAAGAAGATCAGCAGCAACTCTTAAGGTTTCCGATGATGCCTTTTGGCGGTTCATCTTCGAGCTCATCAGATCCTCCTGCTAAGTACAGAAGAGACTCCTCTGGGAGCGGTGACTTTAGAAGATGA

Protein Analysis

317

Amino Acids

35.3

Weight (kDa)

8.69

Isoelectric Point (pI)

68.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 105 - 154 1e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012764)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07310 AT5G61890
fragaria_vesca FvH4_5g09520
malus_domestica MD06G1130100.v1.1 MD06G1130300.v1.1 MD14G1147100.v1.1
prunus_persica Prupe.5G141200_v2.0.a1
pyrus_communis pycom14g12170
rosa_chinensis RchiOBHm_Chr7g0188681
rosa_laevigata RLG00000004671
rosa_multiflora Rmu_sc0001576.1_g000027
rosa_roxburghii Rroxscaffold_3G00266140
rosa_rugosa Rorug06G0492200
rosa_samantha Rh7AG097100 Rh7BG098900 Rh7CG100700 Rh7DG099300
rosa_wichuraiana Rw7G008400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 283
AccBSI CCGCTC 2 cut(s) 176, 933
AccI GTMKAC 1 cut(s) 567
AciI CCGC 4 cut(s) 174, 331, 873, 933
AclWI GGATC 4 cut(s) 51, 362, 509, 890
AfaI GTAC 7 cut(s) 5, 514, 547, 673, 753, 813, 911
AfiI CCNNNNNNNGG 2 cut(s) 45, 872
AflII CTTAAG 1 cut(s) 847
AgsI TTSAA 5 cut(s) 32, 290, 457, 497, 602
AjuI GAANNNNNNNTTGG 2 cut(s) 748, 780
AluBI AGCT 6 cut(s) 293, 446, 475, 578, 587, 888
AluI AGCT 6 cut(s) 293, 446, 475, 578, 587, 888
Alw21I GWGCWC 1 cut(s) 890
Alw26I GTCTC 2 cut(s) 301, 912
AlwI GGATC 4 cut(s) 51, 362, 509, 890
AlwNI CAGNNNCTG 1 cut(s) 419
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 3 cut(s) 62, 335, 780
ApeKI GCWGC 8 cut(s) 353, 380, 419, 422, 443, 587, 823, 838
AseI ATTAAT 1 cut(s) 633
Asp700I GAANNNNTTC 1 cut(s) 492
AspLEI GCGC 1 cut(s) 592
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 2 cut(s) 199, 947
AsuII TTCGAA 2 cut(s) 74, 405
AvaI CYCGRG 1 cut(s) 395
BanII GRGCYC 1 cut(s) 890
Bbv12I GWGCWC 1 cut(s) 890
BbvI GCAGC 8 cut(s) 365, 392, 406, 434, 455, 574, 835, 850
BccI CCATC 1 cut(s) 802
BciVI GTATCC 2 cut(s) 181, 239
BclI TGATCA 1 cut(s) 556
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 2 cut(s) 301, 912
BfaI CTAG 2 cut(s) 510, 519
BfmI CTRYAG 5 cut(s) 276, 316, 420, 613, 818
BfrI CTTAAG 1 cut(s) 847
BfuAI ACCTGC 1 cut(s) 283
BfuI GTATCC 2 cut(s) 181, 239
BglII AGATCT 1 cut(s) 143
BisI GCNGC 8 cut(s) 354, 381, 420, 423, 444, 588, 824, 839
BlpI GCTNAGC 1 cut(s) 471
BlsI GCNGC 8 cut(s) 355, 382, 421, 424, 445, 589, 825, 840
BmcAI AGTACT 1 cut(s) 514
Bme1390I CCNGG 1 cut(s) 128
BmeT110I CYCGRG 1 cut(s) 395
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 2 cut(s) 637, 852
BplI GAGNNNNNCTC 2 cut(s) 378, 410
BpmI CTGGAG 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 471
Bpu14I TTCGAA 2 cut(s) 74, 405
BpuEI CTTGAG 2 cut(s) 121, 279
BpuMI CCSGG 1 cut(s) 128
BsaBI GATNNNNATC 1 cut(s) 555
BsaJI CCNNGG 4 cut(s) 39, 165, 338, 777
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 655, 685
Bsc4I CCNNNNNNNGG 2 cut(s) 45, 872
Bse1I ACTGG 1 cut(s) 26
Bse8I GATNNNNATC 1 cut(s) 555
BseDI CCNNGG 4 cut(s) 39, 165, 338, 777
BseGI GGATG 1 cut(s) 773
BseJI GATNNNNATC 1 cut(s) 555
BseLI CCNNNNNNNGG 2 cut(s) 45, 872
BseMII CTCAG 2 cut(s) 197, 463
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 4 cut(s) 110, 113, 570, 913
BseXI GCAGC 8 cut(s) 365, 392, 406, 434, 455, 574, 835, 850
BshFI GGCC 3 cut(s) 64, 337, 782
BsiHKAI GWGCWC 1 cut(s) 890
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 2 cut(s) 128, 740
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 2 cut(s) 45, 872
BsmAI GTCTC 2 cut(s) 301, 912
BsmFI GGGAC 1 cut(s) 412
BsnI GGCC 3 cut(s) 64, 337, 782
BsoBI CYCGRG 1 cut(s) 395
Bsp119I TTCGAA 2 cut(s) 74, 405
Bsp1286I GDGCHC 1 cut(s) 890
Bsp143I GATC 7 cut(s) 43, 143, 367, 501, 556, 832, 895
Bsp1720I GCTNAGC 1 cut(s) 471
Bsp19I CCATGG 2 cut(s) 165, 338
BspACI CCGC 4 cut(s) 174, 331, 873, 933
BspANI GGCC 3 cut(s) 64, 337, 782
BspCNI CTCAG 2 cut(s) 196, 462
BspHI TCATGA 1 cut(s) 202
BspMAI CTGCAG 2 cut(s) 280, 424
BspMI ACCTGC 1 cut(s) 283
BspPI GGATC 4 cut(s) 51, 362, 509, 890
BspT104I TTCGAA 2 cut(s) 74, 405
BspTI CTTAAG 1 cut(s) 847
BsrBI CCGCTC 2 cut(s) 176, 933
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 4 cut(s) 39, 165, 338, 777
BssMI GATC 7 cut(s) 43, 143, 367, 501, 556, 832, 895
BssT1I CCWWGG 3 cut(s) 39, 165, 338
Bst4CI ACNGT 1 cut(s) 15
Bst6I CTCTTC 3 cut(s) 53, 790, 910
BstAFI CTTAAG 1 cut(s) 847
BstAPI GCANNNNNTGC 2 cut(s) 419, 428
BstBI TTCGAA 2 cut(s) 74, 405
BstDEI CTNAG 4 cut(s) 183, 449, 471, 906
BstDSI CCRYGG 2 cut(s) 165, 338
BstF5I GGATG 1 cut(s) 773
BstHHI GCGC 1 cut(s) 592
BstKTI GATC 7 cut(s) 46, 146, 370, 504, 559, 835, 898
BstMAI GTCTC 2 cut(s) 301, 912
BstMBI GATC 7 cut(s) 43, 143, 367, 501, 556, 832, 895
BstMWI GCNNNNNNNGC 3 cut(s) 419, 428, 584
BstSCI CCNGG 1 cut(s) 126
BstSFI CTRYAG 5 cut(s) 276, 316, 420, 613, 818
BstV1I GCAGC 8 cut(s) 365, 392, 406, 434, 455, 574, 835, 850
BstX2I RGATCY 2 cut(s) 143, 895
BstYI RGATCY 2 cut(s) 143, 895
BsuI GTATCC 2 cut(s) 181, 239
BsuRI GGCC 3 cut(s) 64, 337, 782
BtgI CCRYGG 2 cut(s) 165, 338
BtsCI GGATG 1 cut(s) 773
BtsIMutI CAGTG 1 cut(s) 19
BveI ACCTGC 1 cut(s) 283
CaiI CAGNNNCTG 1 cut(s) 419
CciI TCATGA 1 cut(s) 202
CfoI GCGC 1 cut(s) 592
Csp6I GTAC 7 cut(s) 4, 513, 546, 672, 752, 812, 910
CviAII CATG 7 cut(s) 53, 166, 203, 224, 257, 339, 790
CviQI GTAC 7 cut(s) 4, 513, 546, 672, 752, 812, 910
DdeI CTNAG 4 cut(s) 183, 449, 471, 906
DpnI GATC 7 cut(s) 45, 145, 369, 503, 558, 834, 897
DpnII GATC 7 cut(s) 43, 143, 367, 501, 556, 832, 895
Eam1104I CTCTTC 3 cut(s) 53, 790, 910
EarI CTCTTC 3 cut(s) 53, 790, 910
EciI GGCGGA 1 cut(s) 346
Ecl136II GAGCTC 1 cut(s) 888
Eco130I CCWWGG 3 cut(s) 39, 165, 338
Eco24I GRGCYC 1 cut(s) 890
Eco53kI GAGCTC 1 cut(s) 888
Eco88I CYCGRG 1 cut(s) 395
EcoICRI GAGCTC 1 cut(s) 888
EcoT14I CCWWGG 3 cut(s) 39, 165, 338
EcoT38I GRGCYC 1 cut(s) 890
ErhI CCWWGG 3 cut(s) 39, 165, 338
FaeI CATG 7 cut(s) 56, 169, 206, 227, 260, 342, 793
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 1 cut(s) 412
FatI CATG 7 cut(s) 52, 165, 202, 223, 256, 338, 789
FbaI TGATCA 1 cut(s) 556
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 8 cut(s) 354, 381, 420, 423, 444, 588, 824, 839
FokI GGATG 1 cut(s) 760
FriOI GRGCYC 1 cut(s) 890
Fsp4HI GCNGC 8 cut(s) 354, 381, 420, 423, 444, 588, 824, 839
FspBI CTAG 2 cut(s) 510, 519
GlaI GCGC 1 cut(s) 591
GluI GCNGC 8 cut(s) 354, 381, 420, 423, 444, 588, 824, 839
GsuI CTGGAG 1 cut(s) 567
HaeIII GGCC 3 cut(s) 64, 337, 782
HapII CCGG 2 cut(s) 128, 740
HhaI GCGC 1 cut(s) 592
Hin1II CATG 7 cut(s) 56, 169, 206, 227, 260, 342, 793
Hin6I GCGC 1 cut(s) 590
HinP1I GCGC 1 cut(s) 590
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 5 cut(s) 35, 80, 361, 407, 920
HpaII CCGG 2 cut(s) 128, 740
HphI GGTGA 2 cut(s) 199, 947
Hpy166II GTNNAC 2 cut(s) 133, 568
Hpy188I TCNGA 4 cut(s) 48, 85, 858, 895
Hpy188III TCNNGA 4 cut(s) 29, 203, 365, 397
Hpy8I GTNNAC 2 cut(s) 133, 568
HpyAV CCTTC 2 cut(s) 81, 370
HpyCH4III ACNGT 1 cut(s) 15
HpyCH4V TGCA 5 cut(s) 194, 278, 422, 536, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 419, 428, 584
HpyF3I CTNAG 4 cut(s) 183, 449, 471, 906
Hsp92II CATG 7 cut(s) 56, 169, 206, 227, 260, 342, 793
HspAI GCGC 1 cut(s) 590
Ksp22I TGATCA 1 cut(s) 556
Kzo9I GATC 7 cut(s) 43, 143, 367, 501, 556, 832, 895
LmnI GCTCC 2 cut(s) 583, 930
LpnPI CCDG 9 cut(s) 7, 141, 170, 288, 436, 597, 753, 912, 915
Lsp1109I GCAGC 8 cut(s) 365, 392, 406, 434, 455, 574, 835, 850
LweI GCATC 2 cut(s) 637, 852
MaeI CTAG 2 cut(s) 510, 519
MaeIII GTNAC 2 cut(s) 677, 935
MalI GATC 7 cut(s) 45, 145, 369, 503, 558, 834, 897
MbiI CCGCTC 2 cut(s) 176, 933
MboI GATC 7 cut(s) 43, 143, 367, 501, 556, 832, 895
MflI RGATCY 2 cut(s) 143, 895
MhlI GDGCHC 1 cut(s) 890
MluCI AATT 6 cut(s) 157, 240, 571, 634, 691, 732
MlyI GAGTC 1 cut(s) 914
MroXI GAANNNNTTC 1 cut(s) 492
MseI TTAA 5 cut(s) 477, 633, 690, 747, 848
MspA1I CMGCKG 1 cut(s) 587
MspCI CTTAAG 1 cut(s) 847
MspI CCGG 2 cut(s) 128, 740
MspR9I CCNGG 1 cut(s) 128
MwoI GCNNNNNNNGC 3 cut(s) 419, 428, 584
NciI CCSGG 1 cut(s) 128
NcoI CCATGG 2 cut(s) 165, 338
NdeII GATC 7 cut(s) 43, 143, 367, 501, 556, 832, 895
NlaIII CATG 7 cut(s) 56, 169, 206, 227, 260, 342, 793
NmeAIII GCCGAG 2 cut(s) 382, 758
NmuCI GTSAC 1 cut(s) 935
NspV TTCGAA 2 cut(s) 74, 405
PagI TCATGA 1 cut(s) 202
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PdmI GAANNNNTTC 1 cut(s) 492
PfeI GAWTC 4 cut(s) 35, 80, 361, 407
PkrI GCNGC 8 cut(s) 355, 382, 421, 424, 445, 589, 825, 840
PleI GAGTC 1 cut(s) 914
PpsI GAGTC 1 cut(s) 914
PshBI ATTAAT 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 890
PstI CTGCAG 2 cut(s) 280, 424
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 2 cut(s) 143, 895
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 7 cut(s) 5, 514, 547, 673, 753, 813, 911
RsaNI GTAC 7 cut(s) 4, 513, 546, 672, 752, 812, 910
SacI GAGCTC 1 cut(s) 890
SaqAI TTAA 5 cut(s) 477, 633, 690, 747, 848
SatI GCNGC 8 cut(s) 354, 381, 420, 423, 444, 588, 824, 839
Sau3AI GATC 7 cut(s) 43, 143, 367, 501, 556, 832, 895
ScaI AGTACT 1 cut(s) 514
SchI GAGTC 1 cut(s) 914
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 1 cut(s) 890
SfaNI GCATC 2 cut(s) 637, 852
SfcI CTRYAG 5 cut(s) 276, 316, 420, 613, 818
SfuI TTCGAA 2 cut(s) 74, 405
SmlI CTYRAG 3 cut(s) 136, 294, 847
SmoI CTYRAG 3 cut(s) 136, 294, 847
Sse9I AATT 6 cut(s) 157, 240, 571, 634, 691, 732
SsiI CCGC 4 cut(s) 174, 331, 873, 933
SspMI CTAG 2 cut(s) 510, 519
SstI GAGCTC 1 cut(s) 890
StyD4I CCNGG 1 cut(s) 126
StyI CCWWGG 3 cut(s) 39, 165, 338
TaaI ACNGT 1 cut(s) 15
TaqI TCGA 4 cut(s) 74, 115, 405, 884
TasI AATT 6 cut(s) 157, 240, 571, 634, 691, 732
TatI WGTACW 3 cut(s) 512, 671, 909
TfiI GAWTC 4 cut(s) 35, 80, 361, 407
Tru1I TTAA 5 cut(s) 477, 633, 690, 747, 848
Tru9I TTAA 5 cut(s) 477, 633, 690, 747, 848
TscAI CASTG 1 cut(s) 26
TseFI GTSAC 1 cut(s) 935
TseI GCWGC 8 cut(s) 353, 380, 419, 422, 443, 587, 823, 838
Tsp45I GTSAC 1 cut(s) 935
TspDTI ATGAA 5 cut(s) 93, 212, 219, 453, 867
TspGWI ACGGA 1 cut(s) 735
TspRI CASTG 1 cut(s) 26
Vha464I CTTAAG 1 cut(s) 847
VspI ATTAAT 1 cut(s) 633
XcmI CCANNNNNNNNNTGG 1 cut(s) 345
XmiI GTMKAC 1 cut(s) 567
XmnI GAANNNNTTC 1 cut(s) 492
XspI CTAG 2 cut(s) 510, 519
ZrmI AGTACT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.