MD06G1130300.v1.1
ERF Family

Ethylene-responsive transcription factor ERF114-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012764
Physical Location & Seq
Reverse (-)
27409938 .. 27412959
3022 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1130300.v1.1.491

Sequence Viewer

Length: 480 bp
ATGGACCGAAAACATGGGAAGAGGCCGCTGGCTTCGGTTGAATCAGAGGAAAAAGAGGAGGATCAAATCTTTCCTGTTTACTCGACTCGATCTCAGCAAGACACTTTTGCCATGGTTTCAGCTCTAGCTCATGTGATTGGAAAAAATAGTGACCAAATTAATAACCCAATTGATCAAGTGCAAGGAATAAATCCATTAATAACCTCACAGTCCAGCCCTACAGAGACTCAATCACAACCAGTACTTCAAGATCAAGGAAATTTGAGGAGACAACATTACAGAGGAGTGAGGCGGAGACCATGGGGAAAGTGGGCTGCTGAGATTCGTGACCCGGTGAAGGCAGCCCGAGTGTGGCTGGGCACTTTTGACACAGCTGAGGCTGCAGCACTAGCTTATGATAAAGCAGCACTCAAGTTCAAGGGAAGCAAAGCTAAGCTAAACTTCCCCGAGAGAGTTCAAGGGAGCTCTGAGTCAGCCTAA

Protein Analysis

160

Amino Acids

17.76

Weight (kDa)

9.43

Isoelectric Point (pI)

62.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 92 - 141 5.3e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012764)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07310 AT5G61890
fragaria_vesca FvH4_5g09520
malus_domestica MD06G1130100.v1.1 MD06G1130300.v1.1 MD14G1147100.v1.1
prunus_persica Prupe.5G141200_v2.0.a1
pyrus_communis pycom14g12170
rosa_chinensis RchiOBHm_Chr7g0188681
rosa_laevigata RLG00000004671
rosa_multiflora Rmu_sc0001576.1_g000027
rosa_roxburghii Rroxscaffold_3G00266140
rosa_rugosa Rorug06G0492200
rosa_samantha Rh7AG097100 Rh7BG098900 Rh7CG100700 Rh7DG099300
rosa_wichuraiana Rw7G008400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 26, 292
AclWI GGATC 1 cut(s) 69
AcsI RAATTY 1 cut(s) 259
AfaI GTAC 1 cut(s) 243
AfiI CCNNNNNNNGG 2 cut(s) 337, 351
AgsI TTSAA 4 cut(s) 41, 248, 418, 458
AluBI AGCT 7 cut(s) 122, 128, 374, 392, 431, 436, 465
AluI AGCT 7 cut(s) 122, 128, 374, 392, 431, 436, 465
Alw21I GWGCWC 1 cut(s) 467
Alw26I GTCTC 3 cut(s) 218, 262, 289
AlwI GGATC 1 cut(s) 69
Ama87I CYCGRG 2 cut(s) 345, 446
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 5 cut(s) 314, 341, 380, 383, 404
ApoI RAATTY 1 cut(s) 259
AseI ATTAAT 2 cut(s) 159, 197
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 1 cut(s) 346
AvaI CYCGRG 2 cut(s) 345, 446
AvaII GGWCC 1 cut(s) 4
BaeGI GKGCMC 1 cut(s) 362
BanII GRGCYC 1 cut(s) 467
Bbv12I GWGCWC 1 cut(s) 467
BbvCI CCTCAGC 1 cut(s) 375
BbvI GCAGC 5 cut(s) 301, 353, 367, 395, 416
BclI TGATCA 1 cut(s) 172
BcnI CCSGG 1 cut(s) 332
BcoDI GTCTC 3 cut(s) 218, 262, 289
BfaI CTAG 2 cut(s) 125, 389
BfmI CTRYAG 2 cut(s) 219, 381
BisI GCNGC 6 cut(s) 26, 315, 342, 381, 384, 405
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 6 cut(s) 27, 316, 343, 382, 385, 406
BmcAI AGTACT 1 cut(s) 243
Bme1390I CCNGG 1 cut(s) 332
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 2 cut(s) 345, 446
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 1 cut(s) 332
Bpu10I CCTNAGC 1 cut(s) 375
Bpu1102I GCTNAGC 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 332
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 2 cut(s) 111, 299
Bsc4I CCNNNNNNNGG 2 cut(s) 337, 351
Bse1I ACTGG 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 111, 299
BseLI CCNNNNNNNGG 2 cut(s) 337, 351
BseMII CTCAG 4 cut(s) 107, 309, 366, 459
BseNI ACTGG 1 cut(s) 239
BseRI GAGGAG 3 cut(s) 71, 280, 297
BseSI GKGCMC 1 cut(s) 362
BseXI GCAGC 5 cut(s) 301, 353, 367, 395, 416
BseYI CCCAGC 1 cut(s) 355
BshFI GGCC 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 467
BsiHKCI CYCGRG 2 cut(s) 345, 446
BsiSI CCGG 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 337, 351
BsmAI GTCTC 3 cut(s) 218, 262, 289
BsnI GGCC 1 cut(s) 25
Bso31I GGTCTC 1 cut(s) 289
BsoBI CYCGRG 2 cut(s) 345, 446
Bsp1286I GDGCHC 2 cut(s) 362, 467
Bsp143I GATC 4 cut(s) 61, 89, 172, 250
Bsp1720I GCTNAGC 1 cut(s) 432
Bsp19I CCATGG 2 cut(s) 111, 299
BspACI CCGC 2 cut(s) 26, 292
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 4 cut(s) 106, 310, 367, 460
BspMAI CTGCAG 1 cut(s) 385
BspPI GGATC 1 cut(s) 69
BspTNI GGTCTC 1 cut(s) 289
BsrI ACTGG 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 111, 299
BssMI GATC 4 cut(s) 61, 89, 172, 250
BssT1I CCWWGG 2 cut(s) 111, 299
Bst4CI ACNGT 1 cut(s) 210
Bst6I CTCTTC 1 cut(s) 14
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 5 cut(s) 93, 318, 375, 432, 468
BstDSI CCRYGG 2 cut(s) 111, 299
BstKTI GATC 4 cut(s) 64, 92, 175, 253
BstMAI GTCTC 3 cut(s) 218, 262, 289
BstMBI GATC 4 cut(s) 61, 89, 172, 250
BstMWI GCNNNNNNNGC 2 cut(s) 380, 389
BstSCI CCNGG 1 cut(s) 330
BstSFI CTRYAG 2 cut(s) 219, 381
BstSLI GKGCMC 1 cut(s) 362
BstV1I GCAGC 5 cut(s) 301, 353, 367, 395, 416
BsuRI GGCC 1 cut(s) 25
BtgI CCRYGG 2 cut(s) 111, 299
Cac8I GCNNGC 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 1 cut(s) 242
CviAII CATG 4 cut(s) 14, 112, 131, 300
CviQI GTAC 1 cut(s) 242
DdeI CTNAG 5 cut(s) 93, 318, 375, 432, 468
DpnI GATC 4 cut(s) 63, 91, 174, 252
DpnII GATC 4 cut(s) 61, 89, 172, 250
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
EciI GGCGGA 1 cut(s) 307
Ecl136II GAGCTC 1 cut(s) 465
Eco130I CCWWGG 2 cut(s) 111, 299
Eco24I GRGCYC 1 cut(s) 467
Eco31I GGTCTC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 4
Eco53kI GAGCTC 1 cut(s) 465
Eco88I CYCGRG 2 cut(s) 345, 446
EcoICRI GAGCTC 1 cut(s) 465
EcoT14I CCWWGG 2 cut(s) 111, 299
EcoT38I GRGCYC 1 cut(s) 467
ErhI CCWWGG 2 cut(s) 111, 299
FaeI CATG 4 cut(s) 17, 115, 134, 303
FaiI YATR 5 cut(s) 15, 113, 132, 301, 396
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FatI CATG 4 cut(s) 13, 111, 130, 299
FbaI TGATCA 1 cut(s) 172
Fnu4HI GCNGC 6 cut(s) 26, 315, 342, 381, 384, 405
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 6 cut(s) 26, 315, 342, 381, 384, 405
FspBI CTAG 2 cut(s) 125, 389
GluI GCNGC 6 cut(s) 26, 315, 342, 381, 384, 405
GsaI CCCAGC 1 cut(s) 359
HaeIII GGCC 1 cut(s) 25
HapII CCGG 1 cut(s) 332
Hin1II CATG 4 cut(s) 17, 115, 134, 303
HinfI GANTC 5 cut(s) 41, 85, 226, 322, 470
HpaII CCGG 1 cut(s) 332
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 2 cut(s) 46, 469
Hpy188III TCNNGA 2 cut(s) 248, 326
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 1 cut(s) 331
HpyCH4III ACNGT 1 cut(s) 210
HpyCH4V TGCA 2 cut(s) 181, 383
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 389
HpyF3I CTNAG 5 cut(s) 93, 318, 375, 432, 468
Hsp92II CATG 4 cut(s) 17, 115, 134, 303
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 4 cut(s) 61, 89, 172, 250
LmnI GCTCC 1 cut(s) 462
LpnPI CCDG 6 cut(s) 14, 87, 226, 252, 341, 345
Lsp1109I GCAGC 5 cut(s) 301, 353, 367, 395, 416
MaeI CTAG 2 cut(s) 125, 389
MaeIII GTNAC 2 cut(s) 149, 326
MalI GATC 4 cut(s) 63, 91, 174, 252
MboI GATC 4 cut(s) 61, 89, 172, 250
MboII GAAGA 1 cut(s) 31
MfeI CAATTG 1 cut(s) 168
MhlI GDGCHC 2 cut(s) 362, 467
MluCI AATT 3 cut(s) 156, 168, 259
MlyI GAGTC 3 cut(s) 79, 220, 479
MnlI CCTC 9 cut(s) 15, 40, 49, 52, 214, 258, 275, 282, 370
MseI TTAA 2 cut(s) 159, 197
MspA1I CMGCKG 2 cut(s) 28, 374
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 1 cut(s) 332
MunI CAATTG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 380, 389
NciI CCSGG 1 cut(s) 332
NcoI CCATGG 2 cut(s) 111, 299
NdeII GATC 4 cut(s) 61, 89, 172, 250
NlaIII CATG 4 cut(s) 17, 115, 134, 303
NmuCI GTSAC 2 cut(s) 149, 326
PfeI GAWTC 2 cut(s) 41, 322
PkrI GCNGC 6 cut(s) 27, 316, 343, 382, 385, 406
PleI GAGTC 3 cut(s) 79, 220, 478
PpsI GAGTC 3 cut(s) 79, 220, 478
PshBI ATTAAT 2 cut(s) 159, 197
Psp124BI GAGCTC 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 355
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 385
PvuII CAGCTG 1 cut(s) 374
RsaI GTAC 1 cut(s) 243
RsaNI GTAC 1 cut(s) 242
SacI GAGCTC 1 cut(s) 467
SaqAI TTAA 2 cut(s) 159, 197
SatI GCNGC 6 cut(s) 26, 315, 342, 381, 384, 405
Sau3AI GATC 4 cut(s) 61, 89, 172, 250
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 243
SchI GAGTC 3 cut(s) 79, 220, 479
ScrFI CCNGG 1 cut(s) 332
SduI GDGCHC 2 cut(s) 362, 467
SetI ASST 8 cut(s) 124, 130, 206, 376, 394, 433, 438, 467
SfcI CTRYAG 2 cut(s) 219, 381
SinI GGWCC 1 cut(s) 4
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 3 cut(s) 156, 168, 259
SsiI CCGC 2 cut(s) 26, 292
SspMI CTAG 2 cut(s) 125, 389
SstI GAGCTC 1 cut(s) 467
StyD4I CCNGG 1 cut(s) 330
StyI CCWWGG 2 cut(s) 111, 299
TaaI ACNGT 1 cut(s) 210
TaqI TCGA 2 cut(s) 83, 88
TaqII GACCGA 1 cut(s) 21
TasI AATT 3 cut(s) 156, 168, 259
TatI WGTACW 1 cut(s) 241
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 2 cut(s) 41, 322
Tru1I TTAA 2 cut(s) 159, 197
Tru9I TTAA 2 cut(s) 159, 197
TseFI GTSAC 2 cut(s) 149, 326
TseI GCWGC 5 cut(s) 314, 341, 380, 383, 404
Tsp45I GTSAC 2 cut(s) 149, 326
VpaK11BI GGWCC 1 cut(s) 4
VspI ATTAAT 2 cut(s) 159, 197
XapI RAATTY 1 cut(s) 259
XcmI CCANNNNNNNNNTGG 1 cut(s) 306
XspI CTAG 2 cut(s) 125, 389
ZrmI AGTACT 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.