Rroxscaffold_3G00266140

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012764
Physical Location & Seq
Forward (+)
59516578 .. 59521142
4565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00266140.1

Sequence Viewer

Length: 1095 bp
ATGACATCGCAGGCGGTGCATGAGGTGGATTACGAGCTTAAGGGTGTAGACTATGTAAGTGACGGAGACAAAGAGGATGAGCTAGATGAGGAGGTTCGGGGTGGCTCAAGGAGATTGAGAGCCGGGACCAGACTAATCATCGATACTAATAAACCGGTCGATACAGTGGATCGGAAACATGGGAAGAGGCCACTTCCTTCGAATGAATTGGAGAAGAAAGAGGAGGAGGTGGAGTATCATCGAAATATCTTCCCGGTTTACTCAAGTAGATCTCAACAAGATAATTCTGCCATGGTATCAGCTCTATCTCAGGTGATTTCAAACACTCATGAACAAAACCCGCTTCAACTTGATCATCAAGTTCATGGTATCCCACTAATAATTTCAGCAGATAATCATGTTGAGCAATCTAAACCTGCAGTAGTTCTTCAAGCTCAAGGGAATATGAGACAACACTATAGGGGAGTGAGGCGGAGGCCATGGGGGAAGTGGGCAGCCGAGATTCGTGATCCGCAGAAGGCAGCTCGAGTGTGGCTCGGGACTTTCGAATCAGCAGAGGCTGCAGCACTGGCTTACGACGAAGCAGCTCTCAGGTTCAAAGGAAGCAAGGCTAAGCTTAACTTCCCCGAAAGAGTTCAAGGGATCAGCCCTAGTACTTCTAGTGGTCGCTACCTTGCAATAAGTAGTATCGATGATGATCATCAGCGAGTAGACAATTCAACTCCTCTAGCTGCGCCGATTTCTCAACCAACAACCTATAGTATTCCAAATGATATTAATTTTGATTATGATGCACAACATCTTGGGAGTAGTACTACAAGTAACAGAAATGTTGATTTCGGTGTATCATCATCTCAAAACTACGGACAAGAAAGAATTATGCCGGAGATTAATGTACCAACGACTTCTTCTTCTACATCCTCGGCCTCTTCCATGCCATCTCATCATCAACAAGTACGACTACAGCAGCAAGAAGATCAGCAGCAACTCTTAAGGTTTCCGATGATGCCTTTTGGCGGTTCATCTTCGAGCTCATCAGATCCTCCTGCTAAGTACAGGAGAGACTCCTCTGGGAGCGGTGACTTTAGAAGATGA

Protein Analysis

364

Amino Acids

40.61

Weight (kDa)

6.07

Isoelectric Point (pI)

58.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 152 - 201 1.3e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012764)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07310 AT5G61890
fragaria_vesca FvH4_5g09520
malus_domestica MD06G1130100.v1.1 MD06G1130300.v1.1 MD14G1147100.v1.1
prunus_persica Prupe.5G141200_v2.0.a1
pyrus_communis pycom14g12170
rosa_chinensis RchiOBHm_Chr7g0188681
rosa_laevigata RLG00000004671
rosa_multiflora Rmu_sc0001576.1_g000027
rosa_roxburghii Rroxscaffold_3G00266140
rosa_rugosa Rorug06G0492200
rosa_samantha Rh7AG097100 Rh7BG098900 Rh7CG100700 Rh7DG099300
rosa_wichuraiana Rw7G008400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 424
AccBSI CCGCTC 1 cut(s) 1077
AccI GTMKAC 2 cut(s) 48, 711
AciI CCGC 6 cut(s) 14, 341, 472, 512, 1017, 1077
AclWI GGATC 4 cut(s) 177, 503, 650, 1034
AfaI GTAC 5 cut(s) 655, 814, 897, 957, 1055
AfiI CCNNNNNNNGG 1 cut(s) 1016
AflII CTTAAG 2 cut(s) 38, 991
AgeI ACCGGT 1 cut(s) 154
AgsI TTSAA 6 cut(s) 321, 347, 431, 598, 638, 720
AjuI GAANNNNNNNTTGG 2 cut(s) 892, 924
AluBI AGCT 9 cut(s) 37, 82, 302, 434, 524, 587, 616, 731, 1032
AluI AGCT 9 cut(s) 37, 82, 302, 434, 524, 587, 616, 731, 1032
Alw21I GWGCWC 1 cut(s) 1034
Alw26I GTCTC 3 cut(s) 60, 442, 1056
AlwI GGATC 4 cut(s) 177, 503, 650, 1034
AlwNI CAGNNNCTG 1 cut(s) 560
Ama87I CYCGRG 2 cut(s) 525, 536
AoxI GGCC 3 cut(s) 188, 476, 924
ApeKI GCWGC 8 cut(s) 494, 521, 560, 563, 584, 731, 967, 982
AseI ATTAAT 2 cut(s) 777, 891
AsiGI ACCGGT 1 cut(s) 154
Asp700I GAANNNNTTC 1 cut(s) 633
AspLEI GCGC 1 cut(s) 736
AspS9I GGNCC 1 cut(s) 126
AsuC2I CCSGG 2 cut(s) 124, 254
AsuHPI GGTGA 2 cut(s) 325, 1091
AsuII TTCGAA 2 cut(s) 200, 546
AvaI CYCGRG 2 cut(s) 525, 536
AvaII GGWCC 1 cut(s) 126
BanII GRGCYC 1 cut(s) 1034
Bbv12I GWGCWC 1 cut(s) 1034
BbvI GCAGC 8 cut(s) 506, 533, 547, 575, 596, 718, 979, 994
BccI CCATC 1 cut(s) 946
BciVI GTATCC 1 cut(s) 380
BclI TGATCA 2 cut(s) 352, 697
BcnI CCSGG 2 cut(s) 124, 254
BcoDI GTCTC 3 cut(s) 60, 442, 1056
BfaI CTAG 4 cut(s) 83, 651, 660, 728
BfmI CTRYAG 5 cut(s) 417, 457, 561, 757, 962
BfrI CTTAAG 2 cut(s) 38, 991
BfuAI ACCTGC 1 cut(s) 424
BfuI GTATCC 1 cut(s) 380
BglII AGATCT 1 cut(s) 269
BisI GCNGC 8 cut(s) 495, 522, 561, 564, 585, 732, 968, 983
BlpI GCTNAGC 1 cut(s) 612
BlsI GCNGC 8 cut(s) 496, 523, 562, 565, 586, 733, 969, 984
BmcAI AGTACT 2 cut(s) 655, 814
Bme1390I CCNGG 2 cut(s) 124, 254
Bme18I GGWCC 1 cut(s) 126
BmeT110I CYCGRG 2 cut(s) 525, 536
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 2 cut(s) 124, 254
BmsI GCATC 2 cut(s) 781, 996
BplI GAGNNNNNCTC 2 cut(s) 519, 551
Bpu1102I GCTNAGC 1 cut(s) 612
Bpu14I TTCGAA 2 cut(s) 200, 546
BpuEI CTTGAG 3 cut(s) 91, 247, 420
BpuMI CCSGG 2 cut(s) 124, 254
Bsa29I ATCGAT 2 cut(s) 141, 690
BsaBI GATNNNNATC 2 cut(s) 696, 699
BsaJI CCNNGG 3 cut(s) 291, 479, 921
BsaWI WCCGGW 1 cut(s) 154
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 1 cut(s) 1016
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 573
Bse8I GATNNNNATC 2 cut(s) 696, 699
BseCI ATCGAT 2 cut(s) 141, 690
BseDI CCNNGG 3 cut(s) 291, 479, 921
BseGI GGATG 2 cut(s) 82, 917
BseJI GATNNNNATC 2 cut(s) 696, 699
BseLI CCNNNNNNNGG 1 cut(s) 1016
BseMII CTCAG 2 cut(s) 323, 604
BseNI ACTGG 1 cut(s) 573
BseRI GAGGAG 5 cut(s) 104, 236, 239, 714, 1057
BseXI GCAGC 8 cut(s) 506, 533, 547, 575, 596, 718, 979, 994
Bsh1285I CGRYCG 1 cut(s) 159
BshFI GGCC 3 cut(s) 190, 478, 926
BshTI ACCGGT 1 cut(s) 154
BshVI ATCGAT 2 cut(s) 141, 690
BsiEI CGRYCG 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 1034
BsiHKCI CYCGRG 2 cut(s) 525, 536
BsiSI CCGG 4 cut(s) 123, 155, 254, 884
BslFI GGGAC 2 cut(s) 139, 553
BslI CCNNNNNNNGG 1 cut(s) 1016
BsmAI GTCTC 3 cut(s) 60, 442, 1056
BsmFI GGGAC 2 cut(s) 139, 553
BsnI GGCC 3 cut(s) 190, 478, 926
BsoBI CYCGRG 2 cut(s) 525, 536
Bsp119I TTCGAA 2 cut(s) 200, 546
Bsp1286I GDGCHC 1 cut(s) 1034
Bsp143I GATC 8 cut(s) 169, 269, 352, 508, 642, 697, 976, 1039
Bsp1720I GCTNAGC 1 cut(s) 612
Bsp19I CCATGG 2 cut(s) 291, 479
BspACI CCGC 6 cut(s) 14, 341, 472, 512, 1017, 1077
BspANI GGCC 3 cut(s) 190, 478, 926
BspCNI CTCAG 2 cut(s) 322, 603
BspDI ATCGAT 2 cut(s) 141, 690
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 127
BspMAI CTGCAG 2 cut(s) 421, 565
BspMI ACCTGC 1 cut(s) 424
BspPI GGATC 4 cut(s) 177, 503, 650, 1034
BspT104I TTCGAA 2 cut(s) 200, 546
BspTI CTTAAG 2 cut(s) 38, 991
BsrBI CCGCTC 1 cut(s) 1077
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 1 cut(s) 573
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 3 cut(s) 291, 479, 921
BssMI GATC 8 cut(s) 169, 269, 352, 508, 642, 697, 976, 1039
BssT1I CCWWGG 2 cut(s) 291, 479
Bst4CI ACNGT 1 cut(s) 166
Bst6I CTCTTC 2 cut(s) 179, 934
BstAFI CTTAAG 2 cut(s) 38, 991
BstAPI GCANNNNNTGC 2 cut(s) 16, 560
BstBI TTCGAA 2 cut(s) 200, 546
BstC8I GCNNGC 1 cut(s) 12
BstDEI CTNAG 4 cut(s) 309, 590, 612, 1050
BstDSI CCRYGG 2 cut(s) 291, 479
BstF5I GGATG 2 cut(s) 82, 917
BstHHI GCGC 1 cut(s) 736
BstKTI GATC 8 cut(s) 172, 272, 355, 511, 645, 700, 979, 1042
BstMAI GTCTC 3 cut(s) 60, 442, 1056
BstMBI GATC 8 cut(s) 169, 269, 352, 508, 642, 697, 976, 1039
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 3 cut(s) 16, 560, 569
BstSCI CCNGG 2 cut(s) 122, 252
BstSFI CTRYAG 5 cut(s) 417, 457, 561, 757, 962
BstV1I GCAGC 8 cut(s) 506, 533, 547, 575, 596, 718, 979, 994
BstX2I RGATCY 2 cut(s) 269, 1039
BstYI RGATCY 2 cut(s) 269, 1039
Bsu15I ATCGAT 2 cut(s) 141, 690
BsuI GTATCC 1 cut(s) 380
BsuRI GGCC 3 cut(s) 190, 478, 926
BsuTUI ATCGAT 2 cut(s) 141, 690
BtgI CCRYGG 2 cut(s) 291, 479
BtsCI GGATG 2 cut(s) 82, 917
BtsIMutI CAGTG 2 cut(s) 171, 566
BveI ACCTGC 1 cut(s) 424
Cac8I GCNNGC 1 cut(s) 12
CaiI CAGNNNCTG 1 cut(s) 560
CciI TCATGA 1 cut(s) 328
CfoI GCGC 1 cut(s) 736
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 1 cut(s) 126
ClaI ATCGAT 2 cut(s) 141, 690
Csp6I GTAC 5 cut(s) 654, 813, 896, 956, 1054
CspAI ACCGGT 1 cut(s) 154
CviAII CATG 8 cut(s) 20, 179, 292, 329, 365, 398, 480, 934
CviQI GTAC 5 cut(s) 654, 813, 896, 956, 1054
DdeI CTNAG 4 cut(s) 309, 590, 612, 1050
DpnI GATC 8 cut(s) 171, 271, 354, 510, 644, 699, 978, 1041
DpnII GATC 8 cut(s) 169, 269, 352, 508, 642, 697, 976, 1039
Eam1104I CTCTTC 2 cut(s) 179, 934
EarI CTCTTC 2 cut(s) 179, 934
EciI GGCGGA 1 cut(s) 487
Ecl136II GAGCTC 1 cut(s) 1032
Eco130I CCWWGG 2 cut(s) 291, 479
Eco24I GRGCYC 1 cut(s) 1034
Eco47I GGWCC 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 1032
Eco88I CYCGRG 2 cut(s) 525, 536
EcoICRI GAGCTC 1 cut(s) 1032
EcoT14I CCWWGG 2 cut(s) 291, 479
EcoT38I GRGCYC 1 cut(s) 1034
ErhI CCWWGG 2 cut(s) 291, 479
FaeI CATG 8 cut(s) 23, 182, 295, 332, 368, 401, 483, 937
FalI AAGNNNNNCTT 2 cut(s) 605, 637
FaqI GGGAC 2 cut(s) 139, 553
FatI CATG 8 cut(s) 19, 178, 291, 328, 364, 397, 479, 933
FauI CCCGC 1 cut(s) 348
FbaI TGATCA 2 cut(s) 352, 697
FblI GTMKAC 2 cut(s) 48, 711
Fnu4HI GCNGC 8 cut(s) 495, 522, 561, 564, 585, 732, 968, 983
FokI GGATG 2 cut(s) 89, 904
FriOI GRGCYC 1 cut(s) 1034
Fsp4HI GCNGC 8 cut(s) 495, 522, 561, 564, 585, 732, 968, 983
FspBI CTAG 4 cut(s) 83, 651, 660, 728
GlaI GCGC 1 cut(s) 735
GluI GCNGC 8 cut(s) 495, 522, 561, 564, 585, 732, 968, 983
HaeIII GGCC 3 cut(s) 190, 478, 926
HapII CCGG 4 cut(s) 123, 155, 254, 884
HhaI GCGC 1 cut(s) 736
Hin1II CATG 8 cut(s) 23, 182, 295, 332, 368, 401, 483, 937
Hin6I GCGC 1 cut(s) 734
HinP1I GCGC 1 cut(s) 734
HindIII AAGCTT 1 cut(s) 614
HinfI GANTC 3 cut(s) 502, 548, 1064
HpaII CCGG 4 cut(s) 123, 155, 254, 884
HphI GGTGA 2 cut(s) 325, 1091
Hpy166II GTNNAC 3 cut(s) 49, 259, 712
Hpy188I TCNGA 3 cut(s) 174, 1002, 1039
Hpy188III TCNNGA 3 cut(s) 329, 506, 538
Hpy8I GTNNAC 3 cut(s) 49, 259, 712
Hpy99I CGWCG 1 cut(s) 581
HpyAV CCTTC 2 cut(s) 207, 511
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4V TGCA 5 cut(s) 19, 419, 563, 677, 794
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 560, 569
HpyF3I CTNAG 4 cut(s) 309, 590, 612, 1050
Hsp92II CATG 8 cut(s) 23, 182, 295, 332, 368, 401, 483, 937
HspAI GCGC 1 cut(s) 734
Ksp22I TGATCA 2 cut(s) 352, 697
Kzo9I GATC 8 cut(s) 169, 269, 352, 508, 642, 697, 976, 1039
LmnI GCTCC 1 cut(s) 1074
Lsp1109I GCAGC 8 cut(s) 506, 533, 547, 575, 596, 718, 979, 994
LweI GCATC 2 cut(s) 781, 996
MaeI CTAG 4 cut(s) 83, 651, 660, 728
MaeIII GTNAC 3 cut(s) 59, 821, 1079
MalI GATC 8 cut(s) 171, 271, 354, 510, 644, 699, 978, 1041
MbiI CCGCTC 1 cut(s) 1077
MboI GATC 8 cut(s) 169, 269, 352, 508, 642, 697, 976, 1039
MboII GAAGA 9 cut(s) 196, 226, 241, 419, 900, 903, 921, 986, 1017
MflI RGATCY 2 cut(s) 269, 1039
MhlI GDGCHC 1 cut(s) 1034
MluCI AATT 6 cut(s) 206, 283, 381, 715, 778, 876
MlyI GAGTC 1 cut(s) 1058
MroXI GAANNNNTTC 1 cut(s) 633
MseI TTAA 5 cut(s) 39, 618, 777, 891, 992
MspCI CTTAAG 2 cut(s) 38, 991
MspI CCGG 4 cut(s) 123, 155, 254, 884
MspR9I CCNGG 2 cut(s) 124, 254
MwoI GCNNNNNNNGC 3 cut(s) 16, 560, 569
NciI CCSGG 2 cut(s) 124, 254
NcoI CCATGG 2 cut(s) 291, 479
NdeII GATC 8 cut(s) 169, 269, 352, 508, 642, 697, 976, 1039
NlaIII CATG 8 cut(s) 23, 182, 295, 332, 368, 401, 483, 937
NlaIV GGNNCC 1 cut(s) 127
NmeAIII GCCGAG 2 cut(s) 523, 902
NmuCI GTSAC 2 cut(s) 59, 1079
NspV TTCGAA 2 cut(s) 200, 546
PaeR7I CTCGAG 1 cut(s) 525
PagI TCATGA 1 cut(s) 328
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 2 cut(s) 502, 548
PinAI ACCGGT 1 cut(s) 154
PkrI GCNGC 8 cut(s) 496, 523, 562, 565, 586, 733, 969, 984
PleI GAGTC 1 cut(s) 1058
PpsI GAGTC 1 cut(s) 1058
PshBI ATTAAT 2 cut(s) 777, 891
Psp124BI GAGCTC 1 cut(s) 1034
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 1 cut(s) 126
PspXI VCTCGAGB 1 cut(s) 525
PstI CTGCAG 2 cut(s) 421, 565
PstNI CAGNNNCTG 1 cut(s) 560
PsuI RGATCY 2 cut(s) 269, 1039
RsaI GTAC 5 cut(s) 655, 814, 897, 957, 1055
RsaNI GTAC 5 cut(s) 654, 813, 896, 956, 1054
SacI GAGCTC 1 cut(s) 1034
SaqAI TTAA 5 cut(s) 39, 618, 777, 891, 992
SatI GCNGC 8 cut(s) 495, 522, 561, 564, 585, 732, 968, 983
Sau3AI GATC 8 cut(s) 169, 269, 352, 508, 642, 697, 976, 1039
Sau96I GGNCC 1 cut(s) 126
ScaI AGTACT 2 cut(s) 655, 814
SchI GAGTC 1 cut(s) 1058
ScrFI CCNGG 2 cut(s) 124, 254
SduI GDGCHC 1 cut(s) 1034
SfaNI GCATC 2 cut(s) 781, 996
SfcI CTRYAG 5 cut(s) 417, 457, 561, 757, 962
Sfr274I CTCGAG 1 cut(s) 525
SfuI TTCGAA 2 cut(s) 200, 546
SinI GGWCC 1 cut(s) 126
SlaI CTCGAG 1 cut(s) 525
SmlI CTYRAG 6 cut(s) 38, 106, 262, 435, 525, 991
SmoI CTYRAG 6 cut(s) 38, 106, 262, 435, 525, 991
Sse9I AATT 6 cut(s) 206, 283, 381, 715, 778, 876
SsiI CCGC 6 cut(s) 14, 341, 472, 512, 1017, 1077
SspMI CTAG 4 cut(s) 83, 651, 660, 728
SstI GAGCTC 1 cut(s) 1034
StyD4I CCNGG 2 cut(s) 122, 252
StyI CCWWGG 2 cut(s) 291, 479
TaaI ACNGT 1 cut(s) 166
TaqI TCGA 8 cut(s) 141, 159, 200, 241, 526, 546, 690, 1028
TasI AATT 6 cut(s) 206, 283, 381, 715, 778, 876
TatI WGTACW 3 cut(s) 653, 812, 1053
TfiI GAWTC 2 cut(s) 502, 548
Tru1I TTAA 5 cut(s) 39, 618, 777, 891, 992
Tru9I TTAA 5 cut(s) 39, 618, 777, 891, 992
TscAI CASTG 2 cut(s) 171, 573
TseFI GTSAC 2 cut(s) 59, 1079
TseI GCWGC 8 cut(s) 494, 521, 560, 563, 584, 731, 967, 982
Tsp45I GTSAC 2 cut(s) 59, 1079
TspDTI ATGAA 4 cut(s) 219, 345, 353, 1011
TspGWI ACGGA 2 cut(s) 78, 879
TspRI CASTG 2 cut(s) 171, 573
Vha464I CTTAAG 2 cut(s) 38, 991
VpaK11BI GGWCC 1 cut(s) 126
VspI ATTAAT 2 cut(s) 777, 891
XcmI CCANNNNNNNNNTGG 1 cut(s) 486
XhoI CTCGAG 1 cut(s) 525
XmiI GTMKAC 2 cut(s) 48, 711
XmnI GAANNNNTTC 1 cut(s) 633
XspI CTAG 4 cut(s) 83, 651, 660, 728
ZrmI AGTACT 2 cut(s) 655, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.