Rw7G008400

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012764
Physical Location & Seq
Reverse (-)
7647359 .. 7648382
1024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G008400.1

Sequence Viewer

Length: 672 bp
ATGGTATCAGCTCTATCCCAGGTGATTGCAAACGCTCATGAACAAAACCCGCTTCAACTTGATCATCAAGTTCATGGTATCCCACTAATAATTTCAGCAGATAATCATGTTGTGCAATCTAAACCTGCAGTAGTTTTTCAAGCTCAAGGGAATATGAGACAACACTATAGGGGAGTGAGGCGGAGGCCATGGGGGAAGTGGGCAGCCGAGATTCGTGATCCGCAGAAGGCAGCTCGAGTGTGGCTCGGGACTTTCGAATCAGCAGAGGCTGCAGCACTTGCTTACGATGAAGCAGCTCTCAGGTTCAAAGGAAGCAAGGCTAAGCTTAACTTCCCCGAAAGAGTTCAAGGGATCAGCCCTAGTACTTCTAGTGGTCGCTACCTTGCAATAAGTAGTATCGATCATGATCAGAGAGTAGACAATTCAGCTCCTCCAGCTGCGCCGATTTCTCAACTAACAACTATAATTAATGTACCAACGACTTCTTCTTCTACATCCTCGGGCTCTTCCATGCCATCTCATCATCAAGAAGTACGACTACAGCAGCAAGAAGATCAGCAGCAACTCTTAAGGCTTCCGATGATGCCCTTTGGCGGTTCATCTTCGAGCTCATCAGATCCTCCTGCTAAGTACAGGAGAGACTCCTCTGGGAGCGGTGACTATAGAAGATGA

Protein Analysis

223

Amino Acids

24.42

Weight (kDa)

9.69

Isoelectric Point (pI)

61.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 55 - 104 5.7e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012764)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07310 AT5G61890
fragaria_vesca FvH4_5g09520
malus_domestica MD06G1130100.v1.1 MD06G1130300.v1.1 MD14G1147100.v1.1
prunus_persica Prupe.5G141200_v2.0.a1
pyrus_communis pycom14g12170
rosa_chinensis RchiOBHm_Chr7g0188681
rosa_laevigata RLG00000004671
rosa_multiflora Rmu_sc0001576.1_g000027
rosa_roxburghii Rroxscaffold_3G00266140
rosa_rugosa Rorug06G0492200
rosa_samantha Rh7AG097100 Rh7BG098900 Rh7CG100700 Rh7DG099300
rosa_wichuraiana Rw7G008400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 133
AccBSI CCGCTC 1 cut(s) 654
AccI GTMKAC 1 cut(s) 417
AciI CCGC 5 cut(s) 50, 181, 221, 594, 654
AclWI GGATC 3 cut(s) 212, 359, 611
AfaI GTAC 4 cut(s) 364, 474, 534, 632
AfiI CCNNNNNNNGG 1 cut(s) 593
AflII CTTAAG 1 cut(s) 568
AgsI TTSAA 4 cut(s) 56, 140, 307, 347
AjnI CCWGG 1 cut(s) 18
AjuI GAANNNNNNNTTGG 2 cut(s) 469, 501
AluBI AGCT 8 cut(s) 11, 143, 233, 296, 325, 428, 437, 609
AluI AGCT 8 cut(s) 11, 143, 233, 296, 325, 428, 437, 609
Alw21I GWGCWC 1 cut(s) 611
Alw26I GTCTC 2 cut(s) 151, 633
AlwI GGATC 3 cut(s) 212, 359, 611
AlwNI CAGNNNCTG 1 cut(s) 269
Ama87I CYCGRG 3 cut(s) 234, 245, 499
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 8 cut(s) 203, 230, 269, 272, 293, 437, 544, 559
AseI ATTAAT 1 cut(s) 468
Asp700I GAANNNNTTC 1 cut(s) 342
AspLEI GCGC 1 cut(s) 442
AsuHPI GGTGA 2 cut(s) 34, 668
AsuII TTCGAA 1 cut(s) 255
AvaI CYCGRG 3 cut(s) 234, 245, 499
BanII GRGCYC 2 cut(s) 506, 611
BarI GAAGNNNNNNTAC 2 cut(s) 522, 554
Bbv12I GWGCWC 1 cut(s) 611
BbvI GCAGC 8 cut(s) 215, 242, 256, 284, 305, 424, 556, 571
BccI CCATC 1 cut(s) 523
BciT130I CCWGG 1 cut(s) 20
BciVI GTATCC 1 cut(s) 89
BclI TGATCA 2 cut(s) 61, 406
BcoDI GTCTC 2 cut(s) 151, 633
BfaI CTAG 2 cut(s) 360, 369
BfmI CTRYAG 5 cut(s) 126, 166, 270, 539, 661
BfrI CTTAAG 1 cut(s) 568
BfuAI ACCTGC 1 cut(s) 133
BfuI GTATCC 1 cut(s) 89
BisI GCNGC 8 cut(s) 204, 231, 270, 273, 294, 438, 545, 560
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 8 cut(s) 205, 232, 271, 274, 295, 439, 546, 561
BmcAI AGTACT 1 cut(s) 364
Bme1390I CCNGG 1 cut(s) 20
BmeT110I CYCGRG 3 cut(s) 234, 245, 499
BmrFI CCNGG 1 cut(s) 20
BmsI GCATC 1 cut(s) 573
BplI GAGNNNNNCTC 2 cut(s) 228, 260
BpmI CTGGAG 1 cut(s) 417
Bpu1102I GCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 129
Bsa29I ATCGAT 1 cut(s) 399
BsaBI GATNNNNATC 1 cut(s) 405
BsaJI CCNNGG 3 cut(s) 18, 188, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 593
Bse8I GATNNNNATC 1 cut(s) 405
BseBI CCWGG 1 cut(s) 20
BseCI ATCGAT 1 cut(s) 399
BseDI CCNNGG 3 cut(s) 18, 188, 498
BseGI GGATG 1 cut(s) 494
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 1 cut(s) 593
BseMII CTCAG 1 cut(s) 313
BseRI GAGGAG 2 cut(s) 420, 634
BseXI GCAGC 8 cut(s) 215, 242, 256, 284, 305, 424, 556, 571
BshFI GGCC 1 cut(s) 187
BshVI ATCGAT 1 cut(s) 399
BsiHKAI GWGCWC 1 cut(s) 611
BsiHKCI CYCGRG 3 cut(s) 234, 245, 499
BslFI GGGAC 1 cut(s) 262
BslI CCNNNNNNNGG 1 cut(s) 593
BsmAI GTCTC 2 cut(s) 151, 633
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 1 cut(s) 187
BsoBI CYCGRG 3 cut(s) 234, 245, 499
Bsp119I TTCGAA 1 cut(s) 255
Bsp1286I GDGCHC 2 cut(s) 506, 611
Bsp143I GATC 7 cut(s) 61, 217, 351, 400, 406, 553, 616
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 5 cut(s) 50, 181, 221, 594, 654
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 1 cut(s) 312
BspDI ATCGAT 1 cut(s) 399
BspHI TCATGA 2 cut(s) 37, 403
BspMAI CTGCAG 2 cut(s) 130, 274
BspMI ACCTGC 1 cut(s) 133
BspPI GGATC 3 cut(s) 212, 359, 611
BspQI GCTCTTC 1 cut(s) 511
BspT104I TTCGAA 1 cut(s) 255
BspTI CTTAAG 1 cut(s) 568
BsrBI CCGCTC 1 cut(s) 654
BssECI CCNNGG 3 cut(s) 18, 188, 498
BssMI GATC 7 cut(s) 61, 217, 351, 400, 406, 553, 616
BssT1I CCWWGG 1 cut(s) 188
Bst2UI CCWGG 1 cut(s) 20
Bst6I CTCTTC 1 cut(s) 511
BstAFI CTTAAG 1 cut(s) 568
BstAPI GCANNNNNTGC 2 cut(s) 269, 278
BstBI TTCGAA 1 cut(s) 255
BstDEI CTNAG 3 cut(s) 299, 321, 627
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 494
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 7 cut(s) 64, 220, 354, 403, 409, 556, 619
BstMAI GTCTC 2 cut(s) 151, 633
BstMBI GATC 7 cut(s) 61, 217, 351, 400, 406, 553, 616
BstMWI GCNNNNNNNGC 3 cut(s) 269, 278, 434
BstNI CCWGG 1 cut(s) 20
BstSCI CCNGG 1 cut(s) 18
BstSFI CTRYAG 5 cut(s) 126, 166, 270, 539, 661
BstV1I GCAGC 8 cut(s) 215, 242, 256, 284, 305, 424, 556, 571
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
Bsu15I ATCGAT 1 cut(s) 399
BsuI GTATCC 1 cut(s) 89
BsuRI GGCC 1 cut(s) 187
BsuTUI ATCGAT 1 cut(s) 399
BtgI CCRYGG 1 cut(s) 188
BtsCI GGATG 1 cut(s) 494
BveI ACCTGC 1 cut(s) 133
CaiI CAGNNNCTG 1 cut(s) 269
CciI TCATGA 2 cut(s) 37, 403
CfoI GCGC 1 cut(s) 442
ClaI ATCGAT 1 cut(s) 399
Csp6I GTAC 4 cut(s) 363, 473, 533, 631
CviAII CATG 6 cut(s) 38, 74, 107, 189, 404, 511
CviQI GTAC 4 cut(s) 363, 473, 533, 631
DdeI CTNAG 3 cut(s) 299, 321, 627
DpnI GATC 7 cut(s) 63, 219, 353, 402, 408, 555, 618
DpnII GATC 7 cut(s) 61, 217, 351, 400, 406, 553, 616
Eam1104I CTCTTC 1 cut(s) 511
EarI CTCTTC 1 cut(s) 511
EciI GGCGGA 1 cut(s) 196
Ecl136II GAGCTC 1 cut(s) 609
Eco130I CCWWGG 1 cut(s) 188
Eco24I GRGCYC 2 cut(s) 506, 611
Eco53kI GAGCTC 1 cut(s) 609
Eco88I CYCGRG 3 cut(s) 234, 245, 499
EcoICRI GAGCTC 1 cut(s) 609
EcoRII CCWGG 1 cut(s) 18
EcoT14I CCWWGG 1 cut(s) 188
EcoT38I GRGCYC 2 cut(s) 506, 611
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 6 cut(s) 41, 77, 110, 192, 407, 514
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 262
FatI CATG 6 cut(s) 37, 73, 106, 188, 403, 510
FauI CCCGC 1 cut(s) 57
FbaI TGATCA 2 cut(s) 61, 406
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 8 cut(s) 204, 231, 270, 273, 294, 438, 545, 560
FokI GGATG 1 cut(s) 481
FriOI GRGCYC 2 cut(s) 506, 611
Fsp4HI GCNGC 8 cut(s) 204, 231, 270, 273, 294, 438, 545, 560
FspBI CTAG 2 cut(s) 360, 369
GlaI GCGC 1 cut(s) 441
GluI GCNGC 8 cut(s) 204, 231, 270, 273, 294, 438, 545, 560
GsuI CTGGAG 1 cut(s) 417
HaeIII GGCC 1 cut(s) 187
HhaI GCGC 1 cut(s) 442
Hin1II CATG 6 cut(s) 41, 77, 110, 192, 407, 514
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 3 cut(s) 211, 257, 641
HphI GGTGA 2 cut(s) 34, 668
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 3 cut(s) 411, 579, 616
Hpy188III TCNNGA 5 cut(s) 38, 215, 247, 404, 527
Hpy8I GTNNAC 1 cut(s) 418
HpyAV CCTTC 1 cut(s) 220
HpyCH4V TGCA 5 cut(s) 29, 115, 128, 272, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 278, 434
HpyF3I CTNAG 3 cut(s) 299, 321, 627
Hsp92II CATG 6 cut(s) 41, 77, 110, 192, 407, 514
HspAI GCGC 1 cut(s) 440
Ksp22I TGATCA 2 cut(s) 61, 406
Kzo9I GATC 7 cut(s) 61, 217, 351, 400, 406, 553, 616
LguI GCTCTTC 1 cut(s) 511
LmnI GCTCC 2 cut(s) 433, 651
LpnPI CCDG 8 cut(s) 5, 32, 138, 286, 447, 619, 633, 636
Lsp1109I GCAGC 8 cut(s) 215, 242, 256, 284, 305, 424, 556, 571
LweI GCATC 1 cut(s) 573
MaeI CTAG 2 cut(s) 360, 369
MaeIII GTNAC 1 cut(s) 656
MalI GATC 7 cut(s) 63, 219, 353, 402, 408, 555, 618
MbiI CCGCTC 1 cut(s) 654
MboI GATC 7 cut(s) 61, 217, 351, 400, 406, 553, 616
MboII GAAGA 5 cut(s) 477, 480, 498, 563, 594
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 2 cut(s) 506, 611
MluCI AATT 3 cut(s) 90, 421, 465
MlyI GAGTC 1 cut(s) 635
MnlI CCTC 7 cut(s) 171, 177, 259, 441, 508, 630, 655
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 3 cut(s) 327, 468, 569
MspA1I CMGCKG 1 cut(s) 437
MspCI CTTAAG 1 cut(s) 568
MspR9I CCNGG 1 cut(s) 20
MvaI CCWGG 1 cut(s) 20
MwoI GCNNNNNNNGC 3 cut(s) 269, 278, 434
NcoI CCATGG 1 cut(s) 188
NdeII GATC 7 cut(s) 61, 217, 351, 400, 406, 553, 616
NlaIII CATG 6 cut(s) 41, 77, 110, 192, 407, 514
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 656
NspV TTCGAA 1 cut(s) 255
PaeR7I CTCGAG 1 cut(s) 234
PagI TCATGA 2 cut(s) 37, 403
PciSI GCTCTTC 1 cut(s) 511
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 2 cut(s) 211, 257
PkrI GCNGC 8 cut(s) 205, 232, 271, 274, 295, 439, 546, 561
PleI GAGTC 1 cut(s) 635
PpsI GAGTC 1 cut(s) 635
PshBI ATTAAT 1 cut(s) 468
Psp124BI GAGCTC 1 cut(s) 611
Psp6I CCWGG 1 cut(s) 18
PspGI CCWGG 1 cut(s) 18
PspXI VCTCGAGB 1 cut(s) 234
PstI CTGCAG 2 cut(s) 130, 274
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 1 cut(s) 616
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 4 cut(s) 364, 474, 534, 632
RsaNI GTAC 4 cut(s) 363, 473, 533, 631
SacI GAGCTC 1 cut(s) 611
SapI GCTCTTC 1 cut(s) 511
SaqAI TTAA 3 cut(s) 327, 468, 569
SatI GCNGC 8 cut(s) 204, 231, 270, 273, 294, 438, 545, 560
Sau3AI GATC 7 cut(s) 61, 217, 351, 400, 406, 553, 616
ScaI AGTACT 1 cut(s) 364
SchI GAGTC 1 cut(s) 635
ScrFI CCNGG 1 cut(s) 20
SduI GDGCHC 2 cut(s) 506, 611
SfaNI GCATC 1 cut(s) 573
SfcI CTRYAG 5 cut(s) 126, 166, 270, 539, 661
Sfr274I CTCGAG 1 cut(s) 234
SfuI TTCGAA 1 cut(s) 255
SlaI CTCGAG 1 cut(s) 234
SmlI CTYRAG 3 cut(s) 144, 234, 568
SmoI CTYRAG 3 cut(s) 144, 234, 568
Sse9I AATT 3 cut(s) 90, 421, 465
SsiI CCGC 5 cut(s) 50, 181, 221, 594, 654
SspMI CTAG 2 cut(s) 360, 369
SstI GAGCTC 1 cut(s) 611
StyD4I CCNGG 1 cut(s) 18
StyI CCWWGG 1 cut(s) 188
TaqI TCGA 4 cut(s) 235, 255, 399, 605
TasI AATT 3 cut(s) 90, 421, 465
TatI WGTACW 2 cut(s) 362, 630
TfiI GAWTC 2 cut(s) 211, 257
Tru1I TTAA 3 cut(s) 327, 468, 569
Tru9I TTAA 3 cut(s) 327, 468, 569
TseFI GTSAC 1 cut(s) 656
TseI GCWGC 8 cut(s) 203, 230, 269, 272, 293, 437, 544, 559
Tsp45I GTSAC 1 cut(s) 656
TspDTI ATGAA 4 cut(s) 54, 62, 303, 588
Vha464I CTTAAG 1 cut(s) 568
VspI ATTAAT 1 cut(s) 468
XcmI CCANNNNNNNNNTGG 1 cut(s) 195
XhoI CTCGAG 1 cut(s) 234
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 2 cut(s) 360, 369
ZrmI AGTACT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.