FvH4_5g13010

2-aminoethanethiol dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009768
Physical Location & Seq
Reverse (-)
7344775 .. 7347876
3102 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13010.t1

Sequence Viewer

Length: 825 bp
ATGTTACAGAAGGGTTGCGATAAGTTTCGGAGTTTTGTAGAAAAGAGAGTGAATTTCGGTGTGTTTCGGAGCCACACAAGCATGGCGAGGCAGAGCTCCAAAGTCGAGGCTATCTATGAACTATGTAAAAAGACGTTTACGCCCTCGGGGTCTGCTCCCCCTTCTGCCGAAGCTGTCCAAAAGCTTTCTTCTCTTTTGGACACAGTCCATCCTGCTGATGTTGGACTTCAAGAGGAAAATCCAGATGATGATCGAGGGCTCGGTTTTTTCAGACTTGACAATCTAAATAGGGTTTCTCGATGGACTCAACCAATAACATACTTGAACGTGTATGAAGGAGATACTTTTACGATGTGTATATTCTGTTTCCCTACTTCCTCCGCCATTCCACTTCATGATCACCCTGGCATGACTGTTTTTAGCAAAGTTCTGTATGGTTCTTTGCATGTGAGAGCTTACGATTGGGTGGAACCTTCCCTGGAAAGCAAGGGATCAAATAATTTTCCAGTAAGATTAGCAAAGCTAGCAGTTGACAAAGTTGTGACGGCACCGTGTGGGACATCAGTTTTGTACCCAAATAGTGGGGGGAATCTGCATTCTTTCACTGCAGTCACACCGTGTGCTGTTCTTGACATTCTCACTCCTCCTTACAATGAAGATTTGGGTAGAAAATGCACATACTACCGTGACTACCCATATACTGCCTTCCCTACAGAAATTAAGGTATACAAGGCAAAAGAAGAGGACTATGCATGGCTTGCTGAGACAGAACCTAATATCTATATGCGAGGGGGAAACTACACTGGACCGACTATTCAGGTTTAA

Protein Analysis

275

Amino Acids

30.69

Weight (kDa)

6.31

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 65 - 272 1.6e-71 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 547
AccB7I CCANNNNNTGG 1 cut(s) 581
AccI GTMKAC 1 cut(s) 726
AciI CCGC 1 cut(s) 381
AclWI GGATC 1 cut(s) 499
AcsI RAATTY 1 cut(s) 52
AdeI CACNNNGTG 3 cut(s) 554, 618, 620
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 1 cut(s) 581
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 2 cut(s) 230, 325
AjnI CCWGG 2 cut(s) 403, 477
AluBI AGCT 5 cut(s) 96, 173, 184, 455, 523
AluI AGCT 5 cut(s) 96, 173, 184, 455, 523
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 1 cut(s) 758
AlwI GGATC 1 cut(s) 499
Ama87I CYCGRG 1 cut(s) 145
ApoI RAATTY 1 cut(s) 52
ArsI GACNNNNNNTTYG 2 cut(s) 550, 582
AspS9I GGNCC 1 cut(s) 806
AsuHPI GGTGA 1 cut(s) 392
AsuNHI GCTAGC 1 cut(s) 523
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 1 cut(s) 806
BanI GGYRCC 1 cut(s) 547
BanII GRGCYC 2 cut(s) 98, 261
Bbv12I GWGCWC 1 cut(s) 98
BccI CCATC 2 cut(s) 216, 294
BceAI ACGGC 1 cut(s) 561
BciT130I CCWGG 2 cut(s) 405, 479
BclI TGATCA 1 cut(s) 397
BcoDI GTCTC 1 cut(s) 758
BfaI CTAG 1 cut(s) 524
BfmI CTRYAG 2 cut(s) 606, 711
Bme1390I CCNGG 2 cut(s) 405, 479
Bme18I GGWCC 1 cut(s) 806
BmeT110I CYCGRG 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 806
BmiI GGNNCC 3 cut(s) 71, 471, 549
BmrFI CCNGG 2 cut(s) 405, 479
BmtI GCTAGC 1 cut(s) 527
BsaBI GATNNNNATC 1 cut(s) 249
BsaJI CCNNGG 3 cut(s) 144, 403, 477
Bsc4I CCNNNNNNNGG 1 cut(s) 581
Bse1I ACTGG 2 cut(s) 506, 808
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 2 cut(s) 405, 479
BseDI CCNNGG 3 cut(s) 144, 403, 477
BseGI GGATG 1 cut(s) 208
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 1 cut(s) 581
BseMII CTCAG 1 cut(s) 753
BseNI ACTGG 2 cut(s) 506, 808
BseRI GAGGAG 1 cut(s) 633
BshNI GGYRCC 1 cut(s) 547
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 1 cut(s) 145
BslFI GGGAC 1 cut(s) 571
BslI CCNNNNNNNGG 1 cut(s) 581
BsmAI GTCTC 1 cut(s) 758
BsmFI GGGAC 1 cut(s) 571
BsmI GAATGC 1 cut(s) 595
BsoBI CYCGRG 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 98, 261
Bsp143I GATC 3 cut(s) 250, 397, 491
BspACI CCGC 1 cut(s) 381
BspCNI CTCAG 1 cut(s) 754
BspHI TCATGA 1 cut(s) 394
BspLI GGNNCC 3 cut(s) 71, 471, 549
BspMAI CTGCAG 1 cut(s) 610
BspOI GCTAGC 1 cut(s) 527
BspPI GGATC 1 cut(s) 499
BspT107I GGYRCC 1 cut(s) 547
BsrI ACTGG 2 cut(s) 506, 808
BssECI CCNNGG 3 cut(s) 144, 403, 477
BssMI GATC 3 cut(s) 250, 397, 491
BssNAI GTATAC 1 cut(s) 727
Bst1107I GTATAC 1 cut(s) 727
Bst2UI CCWGG 2 cut(s) 405, 479
Bst4CI ACNGT 5 cut(s) 205, 415, 552, 618, 686
Bst6I CTCTTC 1 cut(s) 735
BstAPI GCANNNNNTGC 1 cut(s) 758
BstC8I GCNNGC 2 cut(s) 525, 759
BstDEI CTNAG 1 cut(s) 762
BstF5I GGATG 1 cut(s) 208
BstKTI GATC 3 cut(s) 253, 400, 494
BstMAI GTCTC 1 cut(s) 758
BstMBI GATC 3 cut(s) 250, 397, 491
BstMWI GCNNNNNNNGC 3 cut(s) 78, 524, 758
BstNI CCWGG 2 cut(s) 405, 479
BstNSI RCATGY 1 cut(s) 449
BstSCI CCNGG 2 cut(s) 403, 477
BstSFI CTRYAG 2 cut(s) 606, 711
BstZ17I GTATAC 1 cut(s) 727
BtsCI GGATG 1 cut(s) 208
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 2 cut(s) 603, 801
Cac8I GCNNGC 2 cut(s) 525, 759
CciI TCATGA 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 806
Csp6I GTAC 1 cut(s) 571
CviAII CATG 5 cut(s) 82, 395, 409, 446, 753
CviJI RGCY 9 cut(s) 72, 96, 110, 173, 184, 259, 455, 523, 757
CviKI_1 RGCY 9 cut(s) 72, 96, 110, 173, 184, 259, 455, 523, 757
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 1 cut(s) 762
DpnI GATC 3 cut(s) 252, 399, 493
DpnII GATC 3 cut(s) 250, 397, 491
DraIII CACNNNGTG 3 cut(s) 554, 618, 620
Eam1104I CTCTTC 1 cut(s) 735
EarI CTCTTC 1 cut(s) 735
EciI GGCGGA 1 cut(s) 370
Ecl136II GAGCTC 1 cut(s) 96
Eco24I GRGCYC 2 cut(s) 98, 261
Eco47I GGWCC 1 cut(s) 806
Eco53kI GAGCTC 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 145
EcoICRI GAGCTC 1 cut(s) 96
EcoRII CCWGG 2 cut(s) 403, 477
EcoT22I ATGCAT 1 cut(s) 754
EcoT38I GRGCYC 2 cut(s) 98, 261
FaeI CATG 5 cut(s) 85, 398, 412, 449, 756
FaqI GGGAC 1 cut(s) 571
FatI CATG 5 cut(s) 81, 394, 408, 445, 752
FbaI TGATCA 1 cut(s) 397
FblI GTMKAC 1 cut(s) 726
FokI GGATG 1 cut(s) 195
FriOI GRGCYC 2 cut(s) 98, 261
FspBI CTAG 1 cut(s) 524
Hin1II CATG 5 cut(s) 85, 398, 412, 449, 756
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 2 cut(s) 304, 589
HphI GGTGA 1 cut(s) 392
Hpy166II GTNNAC 3 cut(s) 138, 532, 727
Hpy188I TCNGA 3 cut(s) 30, 69, 272
Hpy188III TCNNGA 5 cut(s) 230, 242, 297, 395, 629
Hpy8I GTNNAC 3 cut(s) 138, 532, 727
HpyAV CCTTC 5 cut(s) 4, 171, 329, 483, 715
HpyCH4III ACNGT 5 cut(s) 205, 415, 552, 618, 686
HpyCH4IV ACGT 2 cut(s) 134, 327
HpyCH4V TGCA 5 cut(s) 445, 595, 608, 675, 752
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 524, 758
HpyF3I CTNAG 1 cut(s) 762
HpySE526I ACGT 2 cut(s) 134, 327
Hsp92II CATG 5 cut(s) 85, 398, 412, 449, 756
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 3 cut(s) 250, 397, 491
LmnI GCTCC 3 cut(s) 69, 101, 160
LpnPI CCDG 9 cut(s) 225, 255, 390, 417, 464, 491, 519, 789, 803
MaeI CTAG 1 cut(s) 524
MaeII ACGT 2 cut(s) 134, 327
MaeIII GTNAC 4 cut(s) 3, 541, 610, 686
MalI GATC 3 cut(s) 252, 399, 493
MboI GATC 3 cut(s) 250, 397, 491
MboII GAAGA 3 cut(s) 180, 668, 752
MhlI GDGCHC 2 cut(s) 98, 261
MluCI AATT 3 cut(s) 52, 499, 717
MlyI GAGTC 1 cut(s) 298
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 9 cut(s) 81, 100, 154, 226, 248, 388, 654, 736, 782
Mph1103I ATGCAT 1 cut(s) 754
MseI TTAA 2 cut(s) 720, 823
MslI CAYNNNNRTG 1 cut(s) 80
MspR9I CCNGG 2 cut(s) 405, 479
Mva1269I GAATGC 1 cut(s) 595
MvaI CCWGG 2 cut(s) 405, 479
MwoI GCNNNNNNNGC 3 cut(s) 78, 524, 758
NdeII GATC 3 cut(s) 250, 397, 491
NheI GCTAGC 1 cut(s) 523
NlaIII CATG 5 cut(s) 85, 398, 412, 449, 756
NlaIV GGNNCC 3 cut(s) 71, 471, 549
NmuCI GTSAC 3 cut(s) 541, 610, 686
NsiI ATGCAT 1 cut(s) 754
NspI RCATGY 1 cut(s) 449
PagI TCATGA 1 cut(s) 394
PctI GAATGC 1 cut(s) 595
PfeI GAWTC 1 cut(s) 589
PflFI GACNNNGTC 1 cut(s) 203
PflMI CCANNNNNTGG 1 cut(s) 581
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
Psp124BI GAGCTC 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 403, 477
PspGI CCWGG 2 cut(s) 403, 477
PspN4I GGNNCC 3 cut(s) 71, 471, 549
PspPI GGNCC 1 cut(s) 806
PstI CTGCAG 1 cut(s) 610
PsyI GACNNNGTC 1 cut(s) 203
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
RseI CAYNNNNRTG 1 cut(s) 80
SacI GAGCTC 1 cut(s) 98
SaqAI TTAA 2 cut(s) 720, 823
Sau3AI GATC 3 cut(s) 250, 397, 491
Sau96I GGNCC 1 cut(s) 806
SchI GAGTC 1 cut(s) 298
ScrFI CCNGG 2 cut(s) 405, 479
SduI GDGCHC 2 cut(s) 98, 261
SfcI CTRYAG 2 cut(s) 606, 711
SinI GGWCC 1 cut(s) 806
SmiMI CAYNNNNRTG 1 cut(s) 80
Sse9I AATT 3 cut(s) 52, 499, 717
SsiI CCGC 1 cut(s) 381
SspMI CTAG 1 cut(s) 524
SstI GAGCTC 1 cut(s) 98
StyD4I CCNGG 2 cut(s) 403, 477
TaaI ACNGT 5 cut(s) 205, 415, 552, 618, 686
TaiI ACGT 2 cut(s) 137, 330
TaqI TCGA 3 cut(s) 105, 253, 298
TaqII GACCGA 1 cut(s) 823
TasI AATT 3 cut(s) 52, 499, 717
TfiI GAWTC 1 cut(s) 589
Tru1I TTAA 2 cut(s) 720, 823
Tru9I TTAA 2 cut(s) 720, 823
TscAI CASTG 2 cut(s) 610, 808
TseFI GTSAC 3 cut(s) 541, 610, 686
Tsp45I GTSAC 3 cut(s) 541, 610, 686
TspDTI ATGAA 4 cut(s) 132, 348, 383, 669
TspRI CASTG 2 cut(s) 610, 808
Tth111I GACNNNGTC 1 cut(s) 203
Van91I CCANNNNNTGG 1 cut(s) 581
VpaK11BI GGWCC 1 cut(s) 806
XapI RAATTY 1 cut(s) 52
XceI RCATGY 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 726
XspI CTAG 1 cut(s) 524
Zsp2I ATGCAT 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.