MD14G1218600.v1.1

2-aminoethanethiol dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009768
Physical Location & Seq
Forward (+)
30155725 .. 30158747
3023 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1218600.v1.1.491

Sequence Viewer

Length: 813 bp
ATGCTTCCAAAATTTCAGAAAGCAACAGATAAGTTGCGGACGTTTCGAAGCCATACAAGCATGGCGAGGAAGAACTCCAAAGTCCAGGCTCTCTATGAGCTCTGCCAAACGATCTTCACGCCGTCAGGGTCTCCTCCGCCTTCTCCTCCGGCTATCGATAAGCTCTGTTCTGTCCTTGACACGATATCTCCTGCTGATGTTGGGCTTAAAGAGGAAAATCTGGATGACGATCGAGGGCTTGGTTTTTTTGGACTTGACCAACTAAATAGGGTTTCTCGATGGGCTCAACCCATAACATACTTGGATATGTATGAAGGTGATAGTTTTACGATGTGTATATTCTGTTTTCCTACTTCCTCAGTCATTCCGCTTCATGACCATCCTGGGATGACTGTTTTTAGCAAAATTCTCTATGGCTCTTTGCATGTGAGATCTTATGATTGGGTCGAGCCTGCCCAGGAGATCAAGGGACCAAATTACTTTCCAGTAAAATTGGCGAAGCTGGCAGTGGACAAGGTTTTAACGGCACCATGTGCAACATCAGTTTTGTATCCAAGGAGTGGGGGGAATCTGCATAACTTCACGGCAGTCACTCCTTGTGCTGTTCTTGACATTCTCACACCTCCTTACAGAGAAGATGCAGGCAGAAAATGTACCTACTACCGTGATTATCCGTACACCGTGTTTGCTACGGAAAACAAAATTAAAATAGAGGACGGTAAAGAAGAGGAGTATGCATGGCTTGCTGAGACAGAACCGGATAATCTTTACATGCGCTCGGGAAATTACACAGGGCCGTCTATTCAGGTTTAA

Protein Analysis

271

Amino Acids

30.35

Weight (kDa)

5.77

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 58 - 268 1.4e-72 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 526
AccB7I CCANNNNNTGG 1 cut(s) 560
AciI CCGC 3 cut(s) 37, 137, 368
AcsI RAATTY 2 cut(s) 11, 405
AdeI CACNNNGTG 1 cut(s) 533
AfaI GTAC 2 cut(s) 655, 677
AfiI CCNNNNNNNGG 1 cut(s) 560
AjnI CCWGG 3 cut(s) 84, 382, 456
AluBI AGCT 3 cut(s) 100, 163, 502
AluI AGCT 3 cut(s) 100, 163, 502
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 2 cut(s) 135, 743
Ama87I CYCGRG 1 cut(s) 778
AoxI GGCC 1 cut(s) 794
ApoI RAATTY 2 cut(s) 11, 405
AspLEI GCGC 1 cut(s) 777
AspS9I GGNCC 2 cut(s) 470, 794
AsuHPI GGTGA 1 cut(s) 329
AsuII TTCGAA 1 cut(s) 46
AvaI CYCGRG 1 cut(s) 778
AvaII GGWCC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 526
BanII GRGCYC 2 cut(s) 102, 286
Bbv12I GWGCWC 1 cut(s) 102
BccI CCATC 2 cut(s) 273, 387
BceAI ACGGC 4 cut(s) 106, 540, 600, 781
BciT130I CCWGG 3 cut(s) 86, 384, 458
BciVI GTATCC 1 cut(s) 561
BcoDI GTCTC 2 cut(s) 135, 743
BfuI GTATCC 1 cut(s) 561
BglII AGATCT 1 cut(s) 431
Bme1390I CCNGG 3 cut(s) 86, 384, 458
Bme18I GGWCC 1 cut(s) 470
BmeT110I CYCGRG 1 cut(s) 778
BmgT120I GGNCC 2 cut(s) 470, 794
BmiI GGNNCC 2 cut(s) 471, 528
BmrFI CCNGG 3 cut(s) 86, 384, 458
BmsI GCATC 1 cut(s) 628
Bpu14I TTCGAA 1 cut(s) 46
Bsa29I ATCGAT 1 cut(s) 156
BsaBI GATNNNNATC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 135
BsaJI CCNNGG 3 cut(s) 383, 456, 554
BsaWI WCCGGW 1 cut(s) 757
BsaXI ACNNNNNCTCC 2 cut(s) 172, 202
Bsc4I CCNNNNNNNGG 1 cut(s) 560
Bse1I ACTGG 1 cut(s) 485
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 3 cut(s) 86, 384, 458
BseCI ATCGAT 1 cut(s) 156
BseDI CCNNGG 3 cut(s) 383, 456, 554
BseGI GGATG 3 cut(s) 229, 379, 393
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 1 cut(s) 560
BseMII CTCAG 2 cut(s) 372, 738
BseNI ACTGG 1 cut(s) 485
BseRI GAGGAG 3 cut(s) 123, 135, 743
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 1 cut(s) 796
BshNI GGYRCC 1 cut(s) 526
BshVI ATCGAT 1 cut(s) 156
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 778
BsiSI CCGG 2 cut(s) 149, 758
BslFI GGGAC 1 cut(s) 483
BslI CCNNNNNNNGG 1 cut(s) 560
BsmAI GTCTC 2 cut(s) 135, 743
BsmFI GGGAC 1 cut(s) 483
BsnI GGCC 1 cut(s) 796
Bso31I GGTCTC 1 cut(s) 135
BsoBI CYCGRG 1 cut(s) 778
Bsp119I TTCGAA 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 102, 286
Bsp143I GATC 4 cut(s) 111, 229, 431, 462
BspACI CCGC 3 cut(s) 37, 137, 368
BspANI GGCC 1 cut(s) 796
BspCNI CTCAG 2 cut(s) 371, 739
BspDI ATCGAT 1 cut(s) 156
BspHI TCATGA 1 cut(s) 373
BspLI GGNNCC 2 cut(s) 471, 528
BspT104I TTCGAA 1 cut(s) 46
BspT107I GGYRCC 1 cut(s) 526
BspTNI GGTCTC 1 cut(s) 135
BsrI ACTGG 1 cut(s) 485
BssECI CCNNGG 3 cut(s) 383, 456, 554
BssMI GATC 4 cut(s) 111, 229, 431, 462
BssT1I CCWWGG 1 cut(s) 554
Bst2UI CCWGG 3 cut(s) 86, 384, 458
Bst4CI ACNGT 4 cut(s) 394, 665, 682, 719
Bst6I CTCTTC 1 cut(s) 720
BstAPI GCANNNNNTGC 2 cut(s) 533, 743
BstBI TTCGAA 1 cut(s) 46
BstC8I GCNNGC 4 cut(s) 453, 504, 643, 744
BstDEI CTNAG 2 cut(s) 358, 747
BstF5I GGATG 3 cut(s) 229, 379, 393
BstHHI GCGC 1 cut(s) 777
BstKTI GATC 4 cut(s) 114, 232, 434, 465
BstMAI GTCTC 2 cut(s) 135, 743
BstMBI GATC 4 cut(s) 111, 229, 431, 462
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 4 cut(s) 57, 503, 533, 743
BstNI CCWGG 3 cut(s) 86, 384, 458
BstNSI RCATGY 2 cut(s) 428, 775
BstSCI CCNGG 3 cut(s) 84, 382, 456
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
Bsu15I ATCGAT 1 cut(s) 156
BsuI GTATCC 1 cut(s) 561
BsuRI GGCC 1 cut(s) 796
BsuTUI ATCGAT 1 cut(s) 156
BtsCI GGATG 3 cut(s) 229, 379, 393
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 1 cut(s) 513
Cac8I GCNNGC 4 cut(s) 453, 504, 643, 744
CciI TCATGA 1 cut(s) 373
CfoI GCGC 1 cut(s) 777
Cfr13I GGNCC 2 cut(s) 470, 794
ClaI ATCGAT 1 cut(s) 156
Csp6I GTAC 2 cut(s) 654, 676
CviAII CATG 6 cut(s) 61, 374, 425, 531, 738, 772
CviQI GTAC 2 cut(s) 654, 676
DdeI CTNAG 2 cut(s) 358, 747
DpnI GATC 4 cut(s) 113, 231, 433, 464
DpnII GATC 4 cut(s) 111, 229, 431, 462
DraIII CACNNNGTG 1 cut(s) 533
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
EciI GGCGGA 1 cut(s) 126
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 554
Eco24I GRGCYC 2 cut(s) 102, 286
Eco31I GGTCTC 1 cut(s) 135
Eco32I GATATC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 470
Eco53kI GAGCTC 1 cut(s) 100
Eco88I CYCGRG 1 cut(s) 778
EcoICRI GAGCTC 1 cut(s) 100
EcoRII CCWGG 3 cut(s) 84, 382, 456
EcoRV GATATC 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 554
EcoT22I ATGCAT 1 cut(s) 739
EcoT38I GRGCYC 2 cut(s) 102, 286
ErhI CCWWGG 1 cut(s) 554
FaeI CATG 6 cut(s) 64, 377, 428, 534, 741, 775
FaqI GGGAC 1 cut(s) 483
FatI CATG 6 cut(s) 60, 373, 424, 530, 737, 771
FokI GGATG 3 cut(s) 236, 366, 400
FriOI GRGCYC 2 cut(s) 102, 286
GlaI GCGC 1 cut(s) 776
HaeIII GGCC 1 cut(s) 796
HapII CCGG 2 cut(s) 149, 758
HhaI GCGC 1 cut(s) 777
Hin1II CATG 6 cut(s) 64, 377, 428, 534, 741, 775
Hin6I GCGC 1 cut(s) 775
HinP1I GCGC 1 cut(s) 775
HinfI GANTC 1 cut(s) 568
HpaII CCGG 2 cut(s) 149, 758
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 2 cut(s) 511, 678
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 5 cut(s) 221, 276, 374, 608, 780
Hpy8I GTNNAC 2 cut(s) 511, 678
HpyAV CCTTC 2 cut(s) 150, 308
HpyCH4III ACNGT 4 cut(s) 394, 665, 682, 719
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 5 cut(s) 424, 536, 574, 641, 737
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 503, 533, 743
HpyF3I CTNAG 2 cut(s) 358, 747
HpySE526I ACGT 1 cut(s) 41
Hsp92II CATG 6 cut(s) 64, 377, 428, 534, 741, 775
HspAI GCGC 1 cut(s) 775
Kzo9I GATC 4 cut(s) 111, 229, 431, 462
LweI GCATC 1 cut(s) 628
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 589
MalI GATC 4 cut(s) 113, 231, 433, 464
MboI GATC 4 cut(s) 111, 229, 431, 462
MboII GAAGA 4 cut(s) 82, 106, 647, 737
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 2 cut(s) 102, 286
MluCI AATT 6 cut(s) 11, 405, 475, 491, 702, 784
MnlI CCTC 9 cut(s) 60, 144, 156, 205, 227, 367, 633, 706, 721
Mph1103I ATGCAT 1 cut(s) 739
MseI TTAA 4 cut(s) 207, 521, 705, 811
MspI CCGG 2 cut(s) 149, 758
MspR9I CCNGG 3 cut(s) 86, 384, 458
MvaI CCWGG 3 cut(s) 86, 384, 458
MwoI GCNNNNNNNGC 4 cut(s) 57, 503, 533, 743
NdeII GATC 4 cut(s) 111, 229, 431, 462
NlaIII CATG 6 cut(s) 64, 377, 428, 534, 741, 775
NlaIV GGNNCC 2 cut(s) 471, 528
NmuCI GTSAC 1 cut(s) 589
NsiI ATGCAT 1 cut(s) 739
NspI RCATGY 2 cut(s) 428, 775
NspV TTCGAA 1 cut(s) 46
PagI TCATGA 1 cut(s) 373
PfeI GAWTC 1 cut(s) 568
PflMI CCANNNNNTGG 1 cut(s) 560
Ple19I CGATCG 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 102
Psp6I CCWGG 3 cut(s) 84, 382, 456
PspGI CCWGG 3 cut(s) 84, 382, 456
PspN4I GGNNCC 2 cut(s) 471, 528
PspPI GGNCC 2 cut(s) 470, 794
PsuI RGATCY 1 cut(s) 431
PvuI CGATCG 1 cut(s) 232
RsaI GTAC 2 cut(s) 655, 677
RsaNI GTAC 2 cut(s) 654, 676
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 4 cut(s) 207, 521, 705, 811
Sau3AI GATC 4 cut(s) 111, 229, 431, 462
Sau96I GGNCC 2 cut(s) 470, 794
ScrFI CCNGG 3 cut(s) 86, 384, 458
SduI GDGCHC 2 cut(s) 102, 286
SetI ASST 9 cut(s) 44, 102, 165, 319, 504, 519, 625, 659, 810
SfaNI GCATC 1 cut(s) 628
SfuI TTCGAA 1 cut(s) 46
SinI GGWCC 1 cut(s) 470
Sse9I AATT 6 cut(s) 11, 405, 475, 491, 702, 784
SsiI CCGC 3 cut(s) 37, 137, 368
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 3 cut(s) 84, 382, 456
StyI CCWWGG 1 cut(s) 554
TaaI ACNGT 4 cut(s) 394, 665, 682, 719
TaiI ACGT 1 cut(s) 44
TaqI TCGA 5 cut(s) 46, 156, 232, 277, 447
TasI AATT 6 cut(s) 11, 405, 475, 491, 702, 784
TfiI GAWTC 1 cut(s) 568
Tru1I TTAA 4 cut(s) 207, 521, 705, 811
Tru9I TTAA 4 cut(s) 207, 521, 705, 811
TscAI CASTG 1 cut(s) 513
TseFI GTSAC 1 cut(s) 589
Tsp45I GTSAC 1 cut(s) 589
TspDTI ATGAA 2 cut(s) 327, 362
TspGWI ACGGA 2 cut(s) 663, 707
TspRI CASTG 1 cut(s) 513
Van91I CCANNNNNTGG 1 cut(s) 560
VpaK11BI GGWCC 1 cut(s) 470
XapI RAATTY 2 cut(s) 11, 405
XceI RCATGY 2 cut(s) 428, 775
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
Zsp2I ATGCAT 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.