Rw7G005320

2-aminoethanethiol dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009768
Physical Location & Seq
Forward (+)
4523852 .. 4526116
2265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G005320.1

Sequence Viewer

Length: 909 bp
ATGAGTAGAGGTACTGGAAAACCAGGCTCTCTGGAATTTTTTTTTTTTTTTTTCTTCAGTGAGAAAGAAACAGTGGTTTTTCTGAGAATGTTACAAAAAGCTAGCGATAAGTTTCGGGTGTTTTTAGTGAAGAGAGTGCGTTTTGGTGTGTCGCGGAGCCAAACAAGCATGGCGAGGCAGAGCTCCAAAGTCGAGGCTATCTATGAACTCTGCAAAAAGACCTTTTCGCCCTCCGGGTCTCCTCCCCCTTCTGCTGAAGCTGTCAGCAAGCTTTCTTCTCTTTTGGACGCAATCCATCCTGCTGATGTTGGACTTAAAGAGGAAAATCCAGATGATGATCGAGGGCTTGGTTTTTTCAGAGACAATCTAAATAGAGTTTCTCGATGGACTCAACCAATAACATACTTAAATTTATATGAAGGTGATAGTTTTACGATGTGTATATTCTGTTTTCCTACTTCCTCAGTCATTCCACTTCATGATCACCCTGGCATGACTGTTTTTAGCAAAGTTCTGTATGGTTCTTTGCATGTGAGAGCTTACGATTGGGTGGAGCCTTCTCTGGAAAGCAAGGGATCAAATTACTTTCCAGTAAGATTGGCAAAGCTGGCAGCTGACAAAGTTGTAACGGCACCATGTGGGACATCAGTTTTGTACCCAAAGAGTGGGGGGAATCTGCATTCTTTCACTGCAGTCACCCCGTGCGCTGTTCTTGACATTCTCACTCCACCTTACAGAGAAGATTTGGGCAGAAAATGCACATACTATTGTGACTACCCATATACTGCCTTCCCTACAGAAATTAAGGTAGAGGAGGAAAAAGAAGAGGAATATGCATGGCTTGGTGAGACCGAACCTAATATCTACATGCGAGAGGGAAACTACACTGGACCGACTATTCAGATCTAA

Protein Analysis

302

Amino Acids

34.08

Weight (kDa)

6.25

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 94 - 300 3.7e-72 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 631
AccB7I CCANNNNNTGG 1 cut(s) 665
AccII CGCG 1 cut(s) 154
AciI CCGC 1 cut(s) 154
AclWI GGATC 1 cut(s) 583
AcsI RAATTY 2 cut(s) 35, 409
AcuI CTGAAG 2 cut(s) 40, 276
AdeI CACNNNGTG 2 cut(s) 638, 702
AfaI GTAC 2 cut(s) 13, 656
AfiI CCNNNNNNNGG 1 cut(s) 665
AjnI CCWGG 2 cut(s) 22, 487
AluBI AGCT 7 cut(s) 101, 183, 260, 271, 539, 607, 614
AluI AGCT 7 cut(s) 101, 183, 260, 271, 539, 607, 614
Alw21I GWGCWC 1 cut(s) 185
Alw26I GTCTC 3 cut(s) 243, 354, 842
AlwI GGATC 1 cut(s) 583
ApeKI GCWGC 1 cut(s) 611
ApoI RAATTY 2 cut(s) 35, 409
ArsI GACNNNNNNTTYG 2 cut(s) 634, 666
AspLEI GCGC 1 cut(s) 707
AspS9I GGNCC 1 cut(s) 890
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 4 cut(s) 434, 476, 688, 857
AsuNHI GCTAGC 1 cut(s) 101
AvaII GGWCC 1 cut(s) 890
BanI GGYRCC 1 cut(s) 631
BanII GRGCYC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 185
BbvI GCAGC 1 cut(s) 623
BccI CCATC 2 cut(s) 303, 378
BceAI ACGGC 1 cut(s) 645
BciT130I CCWGG 2 cut(s) 24, 489
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 1 cut(s) 235
BcoDI GTCTC 3 cut(s) 243, 354, 842
BfaI CTAG 1 cut(s) 102
BfmI CTRYAG 2 cut(s) 690, 795
BglII AGATCT 1 cut(s) 903
BisI GCNGC 1 cut(s) 612
BlsI GCNGC 1 cut(s) 613
Bme1390I CCNGG 3 cut(s) 24, 235, 489
Bme18I GGWCC 1 cut(s) 890
BmgT120I GGNCC 1 cut(s) 890
BmiI GGNNCC 3 cut(s) 158, 555, 633
BmrFI CCNGG 3 cut(s) 24, 235, 489
BmtI GCTAGC 1 cut(s) 105
BpuMI CCSGG 1 cut(s) 235
BsaBI GATNNNNATC 1 cut(s) 336
BsaI GGTCTC 2 cut(s) 243, 842
BsaJI CCNNGG 1 cut(s) 487
Bsc4I CCNNNNNNNGG 1 cut(s) 665
Bse1I ACTGG 3 cut(s) 19, 590, 892
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 2 cut(s) 24, 489
BseDI CCNNGG 1 cut(s) 487
BseGI GGATG 1 cut(s) 295
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 1 cut(s) 665
BseMII CTCAG 2 cut(s) 74, 477
BseNI ACTGG 3 cut(s) 19, 590, 892
BseRI GAGGAG 2 cut(s) 231, 827
BseXI GCAGC 1 cut(s) 623
Bsh1236I CGCG 1 cut(s) 154
BshNI GGYRCC 1 cut(s) 631
BsiHKAI GWGCWC 1 cut(s) 185
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 1 cut(s) 655
BslI CCNNNNNNNGG 1 cut(s) 665
BsmAI GTCTC 3 cut(s) 243, 354, 842
BsmFI GGGAC 1 cut(s) 655
BsmI GAATGC 1 cut(s) 679
Bso31I GGTCTC 2 cut(s) 243, 842
Bsp1286I GDGCHC 1 cut(s) 185
Bsp143I GATC 4 cut(s) 337, 481, 575, 903
BspACI CCGC 1 cut(s) 154
BspCNI CTCAG 2 cut(s) 75, 476
BspFNI CGCG 1 cut(s) 154
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 3 cut(s) 158, 555, 633
BspMAI CTGCAG 1 cut(s) 694
BspOI GCTAGC 1 cut(s) 105
BspPI GGATC 1 cut(s) 583
BspT107I GGYRCC 1 cut(s) 631
BspTNI GGTCTC 2 cut(s) 243, 842
BsrI ACTGG 3 cut(s) 19, 590, 892
BssECI CCNNGG 1 cut(s) 487
BssMI GATC 4 cut(s) 337, 481, 575, 903
Bst2UI CCWGG 2 cut(s) 24, 489
Bst4CI ACNGT 2 cut(s) 73, 499
Bst6I CTCTTC 2 cut(s) 125, 819
BstAPI GCANNNNNTGC 1 cut(s) 756
BstC8I GCNNGC 3 cut(s) 103, 269, 609
BstDEI CTNAG 2 cut(s) 83, 463
BstF5I GGATG 1 cut(s) 295
BstFNI CGCG 1 cut(s) 154
BstHHI GCGC 1 cut(s) 707
BstKTI GATC 4 cut(s) 340, 484, 578, 906
BstMAI GTCTC 3 cut(s) 243, 354, 842
BstMBI GATC 4 cut(s) 337, 481, 575, 903
BstMWI GCNNNNNNNGC 3 cut(s) 165, 608, 756
BstNI CCWGG 2 cut(s) 24, 489
BstNSI RCATGY 2 cut(s) 533, 871
BstSCI CCNGG 3 cut(s) 22, 233, 487
BstSFI CTRYAG 2 cut(s) 690, 795
BstUI CGCG 1 cut(s) 154
BstV1I GCAGC 1 cut(s) 623
BstX2I RGATCY 1 cut(s) 903
BstYI RGATCY 1 cut(s) 903
BtsCI GGATG 1 cut(s) 295
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 4 cut(s) 64, 78, 687, 885
Cac8I GCNNGC 3 cut(s) 103, 269, 609
CciI TCATGA 1 cut(s) 478
CfoI GCGC 1 cut(s) 707
Cfr13I GGNCC 1 cut(s) 890
CseI GACGC 1 cut(s) 296
Csp6I GTAC 2 cut(s) 12, 655
CviAII CATG 7 cut(s) 169, 479, 493, 530, 636, 837, 868
CviQI GTAC 2 cut(s) 12, 655
DdeI CTNAG 2 cut(s) 83, 463
DpnI GATC 4 cut(s) 339, 483, 577, 905
DpnII GATC 4 cut(s) 337, 481, 575, 903
DraIII CACNNNGTG 2 cut(s) 638, 702
Eam1104I CTCTTC 2 cut(s) 125, 819
EarI CTCTTC 2 cut(s) 125, 819
Ecl136II GAGCTC 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 185
Eco31I GGTCTC 2 cut(s) 243, 842
Eco47I GGWCC 1 cut(s) 890
Eco53kI GAGCTC 1 cut(s) 183
Eco57I CTGAAG 2 cut(s) 40, 276
EcoICRI GAGCTC 1 cut(s) 183
EcoRII CCWGG 2 cut(s) 22, 487
EcoT22I ATGCAT 1 cut(s) 838
EcoT38I GRGCYC 1 cut(s) 185
FaeI CATG 7 cut(s) 172, 482, 496, 533, 639, 840, 871
FaqI GGGAC 1 cut(s) 655
FatI CATG 7 cut(s) 168, 478, 492, 529, 635, 836, 867
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 1 cut(s) 612
FokI GGATG 1 cut(s) 282
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 1 cut(s) 612
FspBI CTAG 1 cut(s) 102
GlaI GCGC 1 cut(s) 706
GluI GCNGC 1 cut(s) 612
HapII CCGG 1 cut(s) 234
HgaI GACGC 1 cut(s) 296
HhaI GCGC 1 cut(s) 707
Hin1II CATG 7 cut(s) 172, 482, 496, 533, 639, 840, 871
Hin6I GCGC 1 cut(s) 705
HinP1I GCGC 1 cut(s) 705
HindIII AAGCTT 1 cut(s) 269
HinfI GANTC 2 cut(s) 388, 673
HpaII CCGG 1 cut(s) 234
HphI GGTGA 4 cut(s) 434, 476, 688, 857
Hpy188I TCNGA 3 cut(s) 84, 359, 903
Hpy188III TCNNGA 6 cut(s) 32, 329, 381, 479, 563, 713
HpyAV CCTTC 4 cut(s) 258, 413, 567, 799
HpyCH4III ACNGT 2 cut(s) 73, 499
HpyCH4V TGCA 6 cut(s) 213, 529, 679, 692, 759, 836
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 608, 756
HpyF3I CTNAG 2 cut(s) 83, 463
Hsp92II CATG 7 cut(s) 172, 482, 496, 533, 639, 840, 871
HspAI GCGC 1 cut(s) 705
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 4 cut(s) 337, 481, 575, 903
LmnI GCTCC 3 cut(s) 156, 188, 553
Lsp1109I GCAGC 1 cut(s) 623
MaeI CTAG 1 cut(s) 102
MaeIII GTNAC 4 cut(s) 90, 625, 694, 770
MalI GATC 4 cut(s) 339, 483, 577, 905
MboI GATC 4 cut(s) 337, 481, 575, 903
MboII GAAGA 5 cut(s) 46, 142, 267, 752, 836
MflI RGATCY 1 cut(s) 903
MhlI GDGCHC 1 cut(s) 185
MluCI AATT 4 cut(s) 35, 409, 580, 801
MlyI GAGTC 1 cut(s) 382
MmeI TCCRAC 1 cut(s) 289
Mph1103I ATGCAT 1 cut(s) 838
MseI TTAA 3 cut(s) 315, 407, 804
MspA1I CMGCKG 1 cut(s) 614
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 3 cut(s) 24, 235, 489
Mva1269I GAATGC 1 cut(s) 679
MvaI CCWGG 2 cut(s) 24, 489
MvnI CGCG 1 cut(s) 154
MwoI GCNNNNNNNGC 3 cut(s) 165, 608, 756
NciI CCSGG 1 cut(s) 235
NdeII GATC 4 cut(s) 337, 481, 575, 903
NheI GCTAGC 1 cut(s) 101
NlaIII CATG 7 cut(s) 172, 482, 496, 533, 639, 840, 871
NlaIV GGNNCC 3 cut(s) 158, 555, 633
NmuCI GTSAC 2 cut(s) 694, 770
NsiI ATGCAT 1 cut(s) 838
NspI RCATGY 2 cut(s) 533, 871
PagI TCATGA 1 cut(s) 478
PctI GAATGC 1 cut(s) 679
PfeI GAWTC 1 cut(s) 673
PflMI CCANNNNNTGG 1 cut(s) 665
PkrI GCNGC 1 cut(s) 613
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
Psp124BI GAGCTC 1 cut(s) 185
Psp6I CCWGG 2 cut(s) 22, 487
PspGI CCWGG 2 cut(s) 22, 487
PspN4I GGNNCC 3 cut(s) 158, 555, 633
PspPI GGNCC 1 cut(s) 890
PstI CTGCAG 1 cut(s) 694
PsuI RGATCY 1 cut(s) 903
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 2 cut(s) 13, 656
RsaNI GTAC 2 cut(s) 12, 655
SacI GAGCTC 1 cut(s) 185
SaqAI TTAA 3 cut(s) 315, 407, 804
SatI GCNGC 1 cut(s) 612
Sau3AI GATC 4 cut(s) 337, 481, 575, 903
Sau96I GGNCC 1 cut(s) 890
SchI GAGTC 1 cut(s) 382
ScrFI CCNGG 3 cut(s) 24, 235, 489
SduI GDGCHC 1 cut(s) 185
SfcI CTRYAG 2 cut(s) 690, 795
SinI GGWCC 1 cut(s) 890
Sse9I AATT 4 cut(s) 35, 409, 580, 801
SsiI CCGC 1 cut(s) 154
SspMI CTAG 1 cut(s) 102
SstI GAGCTC 1 cut(s) 185
StyD4I CCNGG 3 cut(s) 22, 233, 487
TaaI ACNGT 2 cut(s) 73, 499
TaqI TCGA 3 cut(s) 192, 340, 382
TaqII GACCGA 2 cut(s) 866, 907
TasI AATT 4 cut(s) 35, 409, 580, 801
TfiI GAWTC 1 cut(s) 673
Tru1I TTAA 3 cut(s) 315, 407, 804
Tru9I TTAA 3 cut(s) 315, 407, 804
TscAI CASTG 4 cut(s) 64, 78, 694, 892
TseFI GTSAC 2 cut(s) 694, 770
TseI GCWGC 1 cut(s) 611
Tsp45I GTSAC 2 cut(s) 694, 770
TspDTI ATGAA 3 cut(s) 219, 432, 467
TspRI CASTG 4 cut(s) 64, 78, 694, 892
Van91I CCANNNNNTGG 1 cut(s) 665
VpaK11BI GGWCC 1 cut(s) 890
XapI RAATTY 2 cut(s) 35, 409
XceI RCATGY 2 cut(s) 533, 871
XspI CTAG 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.