RLG00000004997

2-aminoethanethiol dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009768
Physical Location & Seq
Reverse (-)
64265010 .. 64267248
2239 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004997

Sequence Viewer

Length: 822 bp
ATGTTACAAAAAGCTAGCGATAAGTTTCGGGTGTTTATAGAGAAGAGAGTGAATTTTGGTGTGCGTCGGAGCCAAGCAAGCATGTCGAGGCAGAGCTCCAAAGTCGAGGCTATCTATGAACTCTGCAAAAAAACCTTTTCGCCCTCGGGGTATCCTCCCCCTTCTGCTGAAGCTGTCAGCAAGATTTCTTCTCTTTTGGACGCAATCCATCCTGCTGATGTTGGGCTTAAAGAGGAAAATCCAGATGATGATCGAGGTCTTGGTTTTTTCAGAGACAATCTAAATAGAGTTTCTCGATGGACTCAACCAATAACATACTTAAATTTATACGAAGGTGATAGTTTTACGATGTGTATATTCTGTTTTCCTACTTCCTCGGTCATTCCACTTCATGATCACCCGGGCATGACTGTTTTTAGCAAAGTTCTGTATGGTTCTTTGCATGTGAGAGCTTACGATTGGGTGGAGCCTTCCCAGGAAAGCAAGGGATCAAATTACTTTCCAGTAAGATTGGCAAAGCTGGCAGCTGACAAAGTTGTAACGGCACCATGTGGGACATCAGTTTTGTACCCAAAGAGTGGGGGGAATCTGCATTCTGTCACTGCAGTCACCCCGTGCGCTGTTCTTGACATTCTCACTCCACCTTACAGAGAAGATTTGGGCAGAAAATGCACATACTATCGTGACTACCCATATACTGCCTTCCCTACAGAAATTAAGGTAGAGGAGGAAAAAGAAGAGGAATATGCATGGCTTGGTGAGACCGAACCTAATATCTACATGCGAGAGGGAAACTACACTGGACCGACTATTCAGATCTAA

Protein Analysis

274

Amino Acids

30.8

Weight (kDa)

6.01

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 65 - 271 7.2e-73 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 544
AccB7I CCANNNNNTGG 1 cut(s) 578
AclWI GGATC 1 cut(s) 496
AcsI RAATTY 2 cut(s) 52, 322
AcuI CTGAAG 1 cut(s) 189
AdeI CACNNNGTG 2 cut(s) 551, 615
AfaI GTAC 1 cut(s) 569
AfiI CCNNNNNNNGG 1 cut(s) 578
AjnI CCWGG 1 cut(s) 474
AluBI AGCT 6 cut(s) 14, 96, 173, 452, 520, 527
AluI AGCT 6 cut(s) 14, 96, 173, 452, 520, 527
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 2 cut(s) 267, 755
AlwI GGATC 1 cut(s) 496
Ama87I CYCGRG 2 cut(s) 145, 400
ApeKI GCWGC 1 cut(s) 524
ApoI RAATTY 2 cut(s) 52, 322
ArsI GACNNNNNNTTYG 2 cut(s) 547, 579
AspLEI GCGC 1 cut(s) 620
AspS9I GGNCC 1 cut(s) 803
AsuC2I CCSGG 2 cut(s) 401, 402
AsuHPI GGTGA 4 cut(s) 347, 389, 601, 770
AsuNHI GCTAGC 1 cut(s) 14
AvaI CYCGRG 2 cut(s) 145, 400
AvaII GGWCC 1 cut(s) 803
BanI GGYRCC 1 cut(s) 544
BanII GRGCYC 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 1 cut(s) 536
BccI CCATC 2 cut(s) 216, 291
BceAI ACGGC 1 cut(s) 558
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BciT130I CCWGG 1 cut(s) 476
BciVI GTATCC 1 cut(s) 162
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 2 cut(s) 401, 402
BcoDI GTCTC 2 cut(s) 267, 755
BfaI CTAG 1 cut(s) 15
BfmI CTRYAG 2 cut(s) 603, 708
BfuI GTATCC 1 cut(s) 162
BglII AGATCT 1 cut(s) 816
BisI GCNGC 1 cut(s) 525
BlsI GCNGC 1 cut(s) 526
Bme1390I CCNGG 3 cut(s) 401, 402, 476
Bme18I GGWCC 1 cut(s) 803
BmeT110I CYCGRG 2 cut(s) 145, 400
BmgT120I GGNCC 1 cut(s) 803
BmiI GGNNCC 3 cut(s) 71, 468, 546
BmrFI CCNGG 3 cut(s) 401, 402, 476
BmtI GCTAGC 1 cut(s) 18
BpuMI CCSGG 2 cut(s) 401, 402
BsaBI GATNNNNATC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 755
BsaJI CCNNGG 4 cut(s) 144, 375, 400, 474
Bsc4I CCNNNNNNNGG 1 cut(s) 578
Bse1I ACTGG 2 cut(s) 503, 805
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 4 cut(s) 144, 375, 400, 474
BseGI GGATG 1 cut(s) 208
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 1 cut(s) 578
BseNI ACTGG 2 cut(s) 503, 805
BseRI GAGGAG 1 cut(s) 740
BseXI GCAGC 1 cut(s) 536
BshNI GGYRCC 1 cut(s) 544
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 2 cut(s) 145, 400
BsiSI CCGG 1 cut(s) 401
BslFI GGGAC 1 cut(s) 568
BslI CCNNNNNNNGG 1 cut(s) 578
BsmAI GTCTC 2 cut(s) 267, 755
BsmFI GGGAC 1 cut(s) 568
BsmI GAATGC 1 cut(s) 592
Bso31I GGTCTC 1 cut(s) 755
BsoBI CYCGRG 2 cut(s) 145, 400
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 4 cut(s) 250, 394, 488, 816
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 3 cut(s) 71, 468, 546
BspMAI CTGCAG 1 cut(s) 607
BspOI GCTAGC 1 cut(s) 18
BspPI GGATC 1 cut(s) 496
BspT107I GGYRCC 1 cut(s) 544
BspTNI GGTCTC 1 cut(s) 755
BsrI ACTGG 2 cut(s) 503, 805
BssECI CCNNGG 4 cut(s) 144, 375, 400, 474
BssMI GATC 4 cut(s) 250, 394, 488, 816
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 1 cut(s) 412
Bst6I CTCTTC 2 cut(s) 38, 732
BstAPI GCANNNNNTGC 1 cut(s) 669
BstC8I GCNNGC 3 cut(s) 16, 79, 522
BstF5I GGATG 1 cut(s) 208
BstHHI GCGC 1 cut(s) 620
BstKTI GATC 4 cut(s) 253, 397, 491, 819
BstMAI GTCTC 2 cut(s) 267, 755
BstMBI GATC 4 cut(s) 250, 394, 488, 816
BstMWI GCNNNNNNNGC 3 cut(s) 78, 521, 669
BstNI CCWGG 1 cut(s) 476
BstNSI RCATGY 3 cut(s) 85, 446, 784
BstSCI CCNGG 3 cut(s) 399, 400, 474
BstSFI CTRYAG 2 cut(s) 603, 708
BstV1I GCAGC 1 cut(s) 536
BstX2I RGATCY 1 cut(s) 816
BstYI RGATCY 1 cut(s) 816
BsuI GTATCC 1 cut(s) 162
BtsCI GGATG 1 cut(s) 208
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 2 cut(s) 600, 798
Cac8I GCNNGC 3 cut(s) 16, 79, 522
CciI TCATGA 1 cut(s) 391
CfoI GCGC 1 cut(s) 620
Cfr13I GGNCC 1 cut(s) 803
Cfr9I CCCGGG 1 cut(s) 400
CseI GACGC 2 cut(s) 53, 209
Csp6I GTAC 1 cut(s) 568
CviAII CATG 7 cut(s) 82, 392, 406, 443, 549, 750, 781
CviQI GTAC 1 cut(s) 568
DpnI GATC 4 cut(s) 252, 396, 490, 818
DpnII GATC 4 cut(s) 250, 394, 488, 816
DraIII CACNNNGTG 2 cut(s) 551, 615
Eam1104I CTCTTC 2 cut(s) 38, 732
EarI CTCTTC 2 cut(s) 38, 732
Ecl136II GAGCTC 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 98
Eco31I GGTCTC 1 cut(s) 755
Eco47I GGWCC 1 cut(s) 803
Eco53kI GAGCTC 1 cut(s) 96
Eco57I CTGAAG 1 cut(s) 189
Eco88I CYCGRG 2 cut(s) 145, 400
EcoICRI GAGCTC 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 474
EcoT22I ATGCAT 1 cut(s) 751
EcoT38I GRGCYC 1 cut(s) 98
FaeI CATG 7 cut(s) 85, 395, 409, 446, 552, 753, 784
FaqI GGGAC 1 cut(s) 568
FatI CATG 7 cut(s) 81, 391, 405, 442, 548, 749, 780
FbaI TGATCA 1 cut(s) 394
Fnu4HI GCNGC 1 cut(s) 525
FokI GGATG 1 cut(s) 195
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 1 cut(s) 525
FspBI CTAG 1 cut(s) 15
GlaI GCGC 1 cut(s) 619
GluI GCNGC 1 cut(s) 525
HapII CCGG 1 cut(s) 401
HgaI GACGC 2 cut(s) 53, 209
HhaI GCGC 1 cut(s) 620
Hin1II CATG 7 cut(s) 85, 395, 409, 446, 552, 753, 784
Hin6I GCGC 1 cut(s) 618
HinP1I GCGC 1 cut(s) 618
HinfI GANTC 2 cut(s) 301, 586
HpaII CCGG 1 cut(s) 401
HphI GGTGA 4 cut(s) 347, 389, 601, 770
Hpy188I TCNGA 3 cut(s) 69, 272, 816
Hpy188III TCNNGA 5 cut(s) 242, 294, 392, 626, 683
Hpy99I CGWCG 1 cut(s) 69
HpyAV CCTTC 4 cut(s) 171, 326, 480, 712
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4V TGCA 6 cut(s) 126, 442, 592, 605, 672, 749
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 521, 669
Hsp92II CATG 7 cut(s) 85, 395, 409, 446, 552, 753, 784
HspAI GCGC 1 cut(s) 618
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 4 cut(s) 250, 394, 488, 816
LmnI GCTCC 3 cut(s) 69, 101, 466
LpnPI CCDG 8 cut(s) 225, 255, 414, 461, 488, 506, 516, 786
Lsp1109I GCAGC 1 cut(s) 536
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 5 cut(s) 3, 538, 598, 607, 683
MalI GATC 4 cut(s) 252, 396, 490, 818
MboI GATC 4 cut(s) 250, 394, 488, 816
MboII GAAGA 4 cut(s) 55, 180, 665, 749
MflI RGATCY 1 cut(s) 816
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 4 cut(s) 52, 322, 493, 714
MlyI GAGTC 1 cut(s) 295
MmeI TCCRAC 1 cut(s) 47
Mph1103I ATGCAT 1 cut(s) 751
MseI TTAA 3 cut(s) 228, 320, 717
MspA1I CMGCKG 1 cut(s) 527
MspI CCGG 1 cut(s) 401
MspR9I CCNGG 3 cut(s) 401, 402, 476
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 1 cut(s) 476
MwoI GCNNNNNNNGC 3 cut(s) 78, 521, 669
NciI CCSGG 2 cut(s) 401, 402
NdeII GATC 4 cut(s) 250, 394, 488, 816
NheI GCTAGC 1 cut(s) 14
NlaIII CATG 7 cut(s) 85, 395, 409, 446, 552, 753, 784
NlaIV GGNNCC 3 cut(s) 71, 468, 546
NmuCI GTSAC 3 cut(s) 598, 607, 683
NsiI ATGCAT 1 cut(s) 751
NspI RCATGY 3 cut(s) 85, 446, 784
PagI TCATGA 1 cut(s) 391
PctI GAATGC 1 cut(s) 592
PfeI GAWTC 1 cut(s) 586
PflMI CCANNNNNTGG 1 cut(s) 578
PkrI GCNGC 1 cut(s) 526
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
Psp124BI GAGCTC 1 cut(s) 98
Psp6I CCWGG 1 cut(s) 474
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 3 cut(s) 71, 468, 546
PspPI GGNCC 1 cut(s) 803
PstI CTGCAG 1 cut(s) 607
PsuI RGATCY 1 cut(s) 816
PvuII CAGCTG 1 cut(s) 527
RsaI GTAC 1 cut(s) 569
RsaNI GTAC 1 cut(s) 568
SacI GAGCTC 1 cut(s) 98
SaqAI TTAA 3 cut(s) 228, 320, 717
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 4 cut(s) 250, 394, 488, 816
Sau96I GGNCC 1 cut(s) 803
SchI GAGTC 1 cut(s) 295
ScrFI CCNGG 3 cut(s) 401, 402, 476
SduI GDGCHC 1 cut(s) 98
SfcI CTRYAG 2 cut(s) 603, 708
SinI GGWCC 1 cut(s) 803
SmaI CCCGGG 1 cut(s) 402
Sse9I AATT 4 cut(s) 52, 322, 493, 714
SspMI CTAG 1 cut(s) 15
SstI GAGCTC 1 cut(s) 98
StyD4I CCNGG 3 cut(s) 399, 400, 474
TaaI ACNGT 1 cut(s) 412
TaqI TCGA 4 cut(s) 86, 105, 253, 295
TaqII GACCGA 3 cut(s) 367, 779, 820
TasI AATT 4 cut(s) 52, 322, 493, 714
TfiI GAWTC 1 cut(s) 586
Tru1I TTAA 3 cut(s) 228, 320, 717
Tru9I TTAA 3 cut(s) 228, 320, 717
TscAI CASTG 2 cut(s) 607, 805
TseFI GTSAC 3 cut(s) 598, 607, 683
TseI GCWGC 1 cut(s) 524
Tsp45I GTSAC 3 cut(s) 598, 607, 683
TspDTI ATGAA 2 cut(s) 132, 380
TspMI CCCGGG 1 cut(s) 400
TspRI CASTG 2 cut(s) 607, 805
Van91I CCANNNNNTGG 1 cut(s) 578
VpaK11BI GGWCC 1 cut(s) 803
XapI RAATTY 2 cut(s) 52, 322
XceI RCATGY 3 cut(s) 85, 446, 784
XmaI CCCGGG 1 cut(s) 400
XspI CTAG 1 cut(s) 15
Zsp2I ATGCAT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.