pycom14g18120

2-aminoethanethiol dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009768
Physical Location & Seq
Forward (+)
19868464 .. 19870299
1836 bp
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UTR
Exon/CDS
Intron
pycom14g18120.1

Sequence Viewer

Length: 579 bp
ATGCTTCCAAAATTTCTGAAAGCAACAGATAAGTTGCGGACGTTTCGAAGCCACACAAGCATGGCGAGGAAGAACTCCAAAGTCCAGGCTCTCTACGAGCTCTGCCAAACGATCTTCACGCCGTCAGGGTCTCCTCCGCCTTCTCCTCCGGCTATCGATAAGCTCTGTTCTGTCCTTGACACGATATCTCCTGCTGATGTTGGGCTTAAAGAGGAAAATCTGGATGACGATCGAGGGCTTGGTTTTTTTGGACTTGACCAACTAAATAGAGTTTCTCGATGGGCTCAACCCATAACATACTTGGATATGTACGAAGGTGATAGTTTTACGATGTGTATATTCCGTTTTCCTACTTCCTCAGTCATTCCGCTTCATGACCATCCTGGGATGACTGTTTTTAGCAGAATTCTCTATGGCTCTTTGCATGTGAGATCTTATGATTGGGTCGAGCCTGCCCAGGTCGATCAAGGGACCAAATTACTTTCCAGTAAGATTGGCGAAGCTGGCAGTGGACAAGGTTTTAACGGCACCATGTGCAACATCAGTTTTGTATCCAAGGAGCGGGGGGAATCTGCATAA

Protein Analysis

193

Amino Acids

21.26

Weight (kDa)

6.41

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCO_ADO PF07847 58 - 158 8.4e-33 PCO_ADO
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 527
AccBSI CCGCTC 1 cut(s) 562
AciI CCGC 4 cut(s) 37, 137, 368, 562
AcsI RAATTY 2 cut(s) 11, 405
AdeI CACNNNGTG 1 cut(s) 534
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 1 cut(s) 561
AjnI CCWGG 3 cut(s) 84, 382, 456
AluBI AGCT 3 cut(s) 100, 163, 503
AluI AGCT 3 cut(s) 100, 163, 503
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 135
ApoI RAATTY 2 cut(s) 11, 405
AspS9I GGNCC 1 cut(s) 471
AsuHPI GGTGA 1 cut(s) 329
AsuII TTCGAA 1 cut(s) 46
AvaII GGWCC 1 cut(s) 471
BanI GGYRCC 1 cut(s) 527
BanII GRGCYC 2 cut(s) 102, 286
Bbv12I GWGCWC 1 cut(s) 102
BccI CCATC 2 cut(s) 273, 387
BceAI ACGGC 2 cut(s) 106, 541
BciT130I CCWGG 3 cut(s) 86, 384, 458
BciVI GTATCC 1 cut(s) 562
BcoDI GTCTC 1 cut(s) 135
BfuI GTATCC 1 cut(s) 562
BglII AGATCT 1 cut(s) 431
Bme1390I CCNGG 3 cut(s) 86, 384, 458
Bme18I GGWCC 1 cut(s) 471
BmgT120I GGNCC 1 cut(s) 471
BmiI GGNNCC 2 cut(s) 472, 529
BmrFI CCNGG 3 cut(s) 86, 384, 458
Bpu14I TTCGAA 1 cut(s) 46
Bsa29I ATCGAT 1 cut(s) 156
BsaBI GATNNNNATC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 135
BsaJI CCNNGG 3 cut(s) 383, 456, 555
BsaXI ACNNNNNCTCC 2 cut(s) 172, 202
Bsc4I CCNNNNNNNGG 1 cut(s) 561
Bse1I ACTGG 1 cut(s) 486
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 3 cut(s) 86, 384, 458
BseCI ATCGAT 1 cut(s) 156
BseDI CCNNGG 3 cut(s) 383, 456, 555
BseGI GGATG 3 cut(s) 229, 379, 393
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 1 cut(s) 561
BseMII CTCAG 1 cut(s) 372
BseNI ACTGG 1 cut(s) 486
BseRI GAGGAG 2 cut(s) 123, 135
Bsh1285I CGRYCG 1 cut(s) 232
BshNI GGYRCC 1 cut(s) 527
BshVI ATCGAT 1 cut(s) 156
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 484
BslI CCNNNNNNNGG 1 cut(s) 561
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 484
Bso31I GGTCTC 1 cut(s) 135
Bsp119I TTCGAA 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 102, 286
Bsp143I GATC 4 cut(s) 111, 229, 431, 463
BspACI CCGC 4 cut(s) 37, 137, 368, 562
BspCNI CTCAG 1 cut(s) 371
BspDI ATCGAT 1 cut(s) 156
BspHI TCATGA 1 cut(s) 373
BspLI GGNNCC 2 cut(s) 472, 529
BspT104I TTCGAA 1 cut(s) 46
BspT107I GGYRCC 1 cut(s) 527
BspTNI GGTCTC 1 cut(s) 135
BsrBI CCGCTC 1 cut(s) 562
BsrI ACTGG 1 cut(s) 486
BssECI CCNNGG 3 cut(s) 383, 456, 555
BssMI GATC 4 cut(s) 111, 229, 431, 463
BssT1I CCWWGG 1 cut(s) 555
Bst2UI CCWGG 3 cut(s) 86, 384, 458
Bst4CI ACNGT 1 cut(s) 394
BstAPI GCANNNNNTGC 1 cut(s) 534
BstBI TTCGAA 1 cut(s) 46
BstC8I GCNNGC 2 cut(s) 453, 505
BstDEI CTNAG 1 cut(s) 358
BstF5I GGATG 3 cut(s) 229, 379, 393
BstKTI GATC 4 cut(s) 114, 232, 434, 466
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 4 cut(s) 111, 229, 431, 463
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 3 cut(s) 57, 504, 534
BstNI CCWGG 3 cut(s) 86, 384, 458
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 3 cut(s) 84, 382, 456
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
Bsu15I ATCGAT 1 cut(s) 156
BsuI GTATCC 1 cut(s) 562
BsuTUI ATCGAT 1 cut(s) 156
BtsCI GGATG 3 cut(s) 229, 379, 393
BtsI GCAGTG 1 cut(s) 514
BtsIMutI CAGTG 1 cut(s) 514
Cac8I GCNNGC 2 cut(s) 453, 505
CciI TCATGA 1 cut(s) 373
Cfr13I GGNCC 1 cut(s) 471
ClaI ATCGAT 1 cut(s) 156
Csp6I GTAC 1 cut(s) 310
CviAII CATG 4 cut(s) 61, 374, 425, 532
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 1 cut(s) 358
DpnI GATC 4 cut(s) 113, 231, 433, 465
DpnII GATC 4 cut(s) 111, 229, 431, 463
DraIII CACNNNGTG 1 cut(s) 534
EciI GGCGGA 1 cut(s) 126
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 555
Eco24I GRGCYC 2 cut(s) 102, 286
Eco31I GGTCTC 1 cut(s) 135
Eco32I GATATC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 471
Eco53kI GAGCTC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 405
EcoRII CCWGG 3 cut(s) 84, 382, 456
EcoRV GATATC 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 555
EcoT38I GRGCYC 2 cut(s) 102, 286
ErhI CCWWGG 1 cut(s) 555
FaeI CATG 4 cut(s) 64, 377, 428, 535
FaqI GGGAC 1 cut(s) 484
FatI CATG 4 cut(s) 60, 373, 424, 531
FauI CCCGC 1 cut(s) 555
FokI GGATG 3 cut(s) 236, 366, 400
FriOI GRGCYC 2 cut(s) 102, 286
HapII CCGG 1 cut(s) 149
Hin1II CATG 4 cut(s) 64, 377, 428, 535
HinfI GANTC 1 cut(s) 569
HpaII CCGG 1 cut(s) 149
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 1 cut(s) 512
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 3 cut(s) 221, 276, 374
Hpy8I GTNNAC 1 cut(s) 512
HpyAV CCTTC 2 cut(s) 150, 308
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 3 cut(s) 424, 537, 575
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 504, 534
HpyF3I CTNAG 1 cut(s) 358
HpySE526I ACGT 1 cut(s) 41
Hsp92II CATG 4 cut(s) 64, 377, 428, 535
Kzo9I GATC 4 cut(s) 111, 229, 431, 463
LmnI GCTCC 1 cut(s) 559
MaeII ACGT 1 cut(s) 41
MalI GATC 4 cut(s) 113, 231, 433, 465
MbiI CCGCTC 1 cut(s) 562
MboI GATC 4 cut(s) 111, 229, 431, 463
MboII GAAGA 2 cut(s) 82, 106
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 2 cut(s) 102, 286
MluCI AATT 3 cut(s) 11, 405, 476
MnlI CCTC 6 cut(s) 60, 144, 156, 205, 227, 367
MseI TTAA 2 cut(s) 207, 522
MslI CAYNNNNRTG 1 cut(s) 59
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 3 cut(s) 86, 384, 458
MvaI CCWGG 3 cut(s) 86, 384, 458
MwoI GCNNNNNNNGC 3 cut(s) 57, 504, 534
NdeII GATC 4 cut(s) 111, 229, 431, 463
NlaIII CATG 4 cut(s) 64, 377, 428, 535
NlaIV GGNNCC 2 cut(s) 472, 529
NspI RCATGY 1 cut(s) 428
NspV TTCGAA 1 cut(s) 46
PagI TCATGA 1 cut(s) 373
PfeI GAWTC 1 cut(s) 569
Ple19I CGATCG 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 102
Psp6I CCWGG 3 cut(s) 84, 382, 456
PspGI CCWGG 3 cut(s) 84, 382, 456
PspN4I GGNNCC 2 cut(s) 472, 529
PspPI GGNCC 1 cut(s) 471
PsuI RGATCY 1 cut(s) 431
PvuI CGATCG 1 cut(s) 232
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
RseI CAYNNNNRTG 1 cut(s) 59
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 2 cut(s) 207, 522
Sau3AI GATC 4 cut(s) 111, 229, 431, 463
Sau96I GGNCC 1 cut(s) 471
ScrFI CCNGG 3 cut(s) 86, 384, 458
SduI GDGCHC 2 cut(s) 102, 286
SetI ASST 7 cut(s) 44, 102, 165, 319, 462, 505, 520
SfuI TTCGAA 1 cut(s) 46
SinI GGWCC 1 cut(s) 471
SmiMI CAYNNNNRTG 1 cut(s) 59
Sse9I AATT 3 cut(s) 11, 405, 476
SsiI CCGC 4 cut(s) 37, 137, 368, 562
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 3 cut(s) 84, 382, 456
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 1 cut(s) 44
TaqI TCGA 6 cut(s) 46, 156, 232, 277, 447, 462
TasI AATT 3 cut(s) 11, 405, 476
TfiI GAWTC 1 cut(s) 569
Tru1I TTAA 2 cut(s) 207, 522
Tru9I TTAA 2 cut(s) 207, 522
TscAI CASTG 1 cut(s) 514
TspDTI ATGAA 1 cut(s) 362
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 1 cut(s) 514
VpaK11BI GGWCC 1 cut(s) 471
XapI RAATTY 2 cut(s) 11, 405
XceI RCATGY 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.