Rorug06G0463600

2-aminoethanethiol dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009768
Physical Location & Seq
Forward (+)
58880266 .. 58880643
378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0463600.1

Sequence Viewer

Length: 378 bp
ATGGCCACAGCCGACTTTCATCACAAGCACGACGTCGATCAGTACTCGTATCCGTCCTCACGGGGCCAGACTAGCATAACCAGGTTCTTAACCGCCGGCGCAATTGGCACCACGCTTTTAGGCTTGTCCGCGTTAACTTTAACCGGGACCGTGATGGCCCTGATCCTGGCCACCCCAGTTCTAGTGCTGTTTAGTCCGGTCCTAGTCCCGGCGGCAATAGTCGTGGTCCTAGTAGCAGCTGGTTTTGTTTTCTCCGGCGGGTGTGGCGTGGCTGCCATCGGGGCGCTGACGTGGTTGTACAAGTATGTATCCAACTACGTCTCTACCAGCAGAAGGGATTACACTTATGGGGGGTCTTACGCTCAATTTGGTCTATGA

Protein Analysis

125

Amino Acids

13.13

Weight (kDa)

8.82

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oleosin PF01277 26 - 106 3.3e-29 Oleosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 36
AccB1I GGYRCC 1 cut(s) 107
AccII CGCG 1 cut(s) 131
AciI CCGC 4 cut(s) 93, 129, 212, 258
AclWI GGATC 1 cut(s) 157
AcoI YGGCCR 2 cut(s) 3, 168
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 2 cut(s) 44, 299
AfiI CCNNNNNNNGG 3 cut(s) 166, 208, 333
AjiI CACGTC 1 cut(s) 291
AjnI CCWGG 2 cut(s) 80, 165
AluBI AGCT 1 cut(s) 239
AluI AGCT 1 cut(s) 239
Alw26I GTCTC 1 cut(s) 325
AlwI GGATC 1 cut(s) 157
AoxI GGCC 4 cut(s) 3, 64, 156, 168
ApeKI GCWGC 2 cut(s) 236, 272
AspLEI GCGC 2 cut(s) 101, 286
AspS9I GGNCC 5 cut(s) 64, 147, 157, 199, 226
AsuC2I CCSGG 2 cut(s) 145, 209
AvaII GGWCC 3 cut(s) 147, 199, 226
BalI TGGCCA 2 cut(s) 5, 170
BanI GGYRCC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 248, 259
BccI CCATC 2 cut(s) 148, 284
BciT130I CCWGG 2 cut(s) 82, 167
BciVI GTATCC 2 cut(s) 60, 319
BcnI CCSGG 2 cut(s) 145, 209
BcoDI GTCTC 1 cut(s) 325
BfaI CTAG 4 cut(s) 72, 182, 203, 230
BfoI RGCGCY 1 cut(s) 287
BfuI GTATCC 2 cut(s) 60, 319
BglI GCCNNNNNGGC 1 cut(s) 281
BisI GCNGC 3 cut(s) 213, 237, 273
BlsI GCNGC 3 cut(s) 214, 238, 274
BmcAI AGTACT 1 cut(s) 44
Bme1390I CCNGG 4 cut(s) 82, 145, 167, 209
Bme18I GGWCC 3 cut(s) 147, 199, 226
BmgBI CACGTC 1 cut(s) 291
BmgT120I GGNCC 5 cut(s) 64, 147, 157, 199, 226
BmiI GGNNCC 3 cut(s) 65, 109, 148
BmrFI CCNGG 4 cut(s) 82, 145, 167, 209
BmrI ACTGGG 1 cut(s) 170
BmuI ACTGGG 1 cut(s) 170
BpuMI CCSGG 2 cut(s) 145, 209
BsaHI GRCGYC 1 cut(s) 33
BsaWI WCCGGW 1 cut(s) 196
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 208, 333
Bse118I RCCGGY 1 cut(s) 95
Bse1I ACTGG 1 cut(s) 176
BseBI CCWGG 2 cut(s) 82, 167
BseLI CCNNNNNNNGG 3 cut(s) 166, 208, 333
BseNI ACTGG 1 cut(s) 176
BseXI GCAGC 2 cut(s) 248, 259
Bsh1236I CGCG 1 cut(s) 131
BshFI GGCC 4 cut(s) 5, 66, 158, 170
BshNI GGYRCC 1 cut(s) 107
BsiSI CCGG 5 cut(s) 96, 144, 197, 209, 255
BslFI GGGAC 2 cut(s) 160, 191
BslI CCNNNNNNNGG 3 cut(s) 166, 208, 333
BsmAI GTCTC 1 cut(s) 325
BsmBI CGTCTC 1 cut(s) 325
BsmFI GGGAC 2 cut(s) 160, 191
BsnI GGCC 4 cut(s) 5, 66, 158, 170
Bsp1407I TGTACA 1 cut(s) 297
Bsp143I GATC 2 cut(s) 37, 162
BspACI CCGC 4 cut(s) 93, 129, 212, 258
BspANI GGCC 4 cut(s) 5, 66, 158, 170
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 3 cut(s) 65, 109, 148
BspPI GGATC 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 107
BsrFI RCCGGY 1 cut(s) 95
BsrGI TGTACA 1 cut(s) 297
BsrI ACTGG 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 95
BssMI GATC 2 cut(s) 37, 162
BssNI GRCGYC 1 cut(s) 33
Bst2UI CCWGG 2 cut(s) 82, 167
Bst4CI ACNGT 1 cut(s) 151
BstACI GRCGYC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 297
BstC8I GCNNGC 1 cut(s) 97
BstFNI CGCG 1 cut(s) 131
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 2 cut(s) 101, 286
BstKTI GATC 2 cut(s) 40, 165
BstMAI GTCTC 1 cut(s) 325
BstMBI GATC 2 cut(s) 37, 162
BstMWI GCNNNNNNNGC 4 cut(s) 72, 105, 264, 281
BstNI CCWGG 2 cut(s) 82, 167
BstSCI CCNGG 4 cut(s) 80, 143, 165, 207
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 2 cut(s) 248, 259
BsuI GTATCC 2 cut(s) 60, 319
BsuRI GGCC 4 cut(s) 5, 66, 158, 170
BtrI CACGTC 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 97
CfoI GCGC 2 cut(s) 101, 286
Cfr10I RCCGGY 1 cut(s) 95
Cfr13I GGNCC 5 cut(s) 64, 147, 157, 199, 226
CsiI ACCWGGT 1 cut(s) 80
Csp6I GTAC 2 cut(s) 43, 298
CspCI CAANNNNNGTGG 2 cut(s) 204, 239
CviJI RGCY 8 cut(s) 5, 11, 66, 123, 158, 170, 239, 272
CviKI_1 RGCY 8 cut(s) 5, 11, 66, 123, 158, 170, 239, 272
CviQI GTAC 2 cut(s) 43, 298
DpnI GATC 2 cut(s) 39, 164
DpnII GATC 2 cut(s) 37, 162
EaeI YGGCCR 2 cut(s) 3, 168
Eco47I GGWCC 3 cut(s) 147, 199, 226
EcoRII CCWGG 2 cut(s) 80, 165
Esp3I CGTCTC 1 cut(s) 325
FaiI YATR 4 cut(s) 77, 306, 348, 376
FaqI GGGAC 2 cut(s) 160, 191
FauI CCCGC 1 cut(s) 251
Fnu4HI GCNGC 3 cut(s) 213, 237, 273
Fsp4HI GCNGC 3 cut(s) 213, 237, 273
FspBI CTAG 4 cut(s) 72, 182, 203, 230
GlaI GCGC 2 cut(s) 100, 285
GluI GCNGC 3 cut(s) 213, 237, 273
HaeII RGCGCY 1 cut(s) 287
HaeIII GGCC 4 cut(s) 5, 66, 158, 170
HapII CCGG 5 cut(s) 96, 144, 197, 209, 255
HhaI GCGC 2 cut(s) 101, 286
Hin1I GRCGYC 1 cut(s) 33
Hin6I GCGC 2 cut(s) 99, 284
HinP1I GCGC 2 cut(s) 99, 284
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HpaI GTTAAC 1 cut(s) 135
HpaII CCGG 5 cut(s) 96, 144, 197, 209, 255
Hpy166II GTNNAC 1 cut(s) 135
Hpy8I GTNNAC 1 cut(s) 135
Hpy99I CGWCG 2 cut(s) 35, 38
HpyAV CCTTC 1 cut(s) 327
HpyCH4III ACNGT 1 cut(s) 151
HpyCH4IV ACGT 3 cut(s) 33, 290, 318
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 105, 264, 281
HpySE526I ACGT 3 cut(s) 33, 290, 318
Hsp92I GRCGYC 1 cut(s) 33
HspAI GCGC 2 cut(s) 99, 284
KroI GCCGGC 1 cut(s) 95
KroNI GCCGGC 1 cut(s) 97
KspAI GTTAAC 1 cut(s) 135
Kzo9I GATC 2 cut(s) 37, 162
Lsp1109I GCAGC 2 cut(s) 248, 259
MabI ACCWGGT 1 cut(s) 80
MaeI CTAG 4 cut(s) 72, 182, 203, 230
MaeII ACGT 3 cut(s) 33, 290, 318
MalI GATC 2 cut(s) 39, 164
MboI GATC 2 cut(s) 37, 162
MfeI CAATTG 1 cut(s) 102
MlsI TGGCCA 2 cut(s) 5, 170
MluCI AATT 2 cut(s) 102, 365
MluNI TGGCCA 2 cut(s) 5, 170
MmeI TCCRAC 1 cut(s) 336
MnlI CCTC 1 cut(s) 67
Mox20I TGGCCA 2 cut(s) 5, 170
MreI CGCCGGCG 1 cut(s) 95
MroNI GCCGGC 1 cut(s) 95
MscI TGGCCA 2 cut(s) 5, 170
MseI TTAA 3 cut(s) 89, 134, 140
Msp20I TGGCCA 2 cut(s) 5, 170
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 5 cut(s) 96, 144, 197, 209, 255
MspR9I CCNGG 4 cut(s) 82, 145, 167, 209
MunI CAATTG 1 cut(s) 102
MvaI CCWGG 2 cut(s) 82, 167
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 4 cut(s) 72, 105, 264, 281
NaeI GCCGGC 1 cut(s) 97
NciI CCSGG 2 cut(s) 145, 209
NdeII GATC 2 cut(s) 37, 162
NgoMIV GCCGGC 1 cut(s) 95
NlaIV GGNNCC 3 cut(s) 65, 109, 148
PdiI GCCGGC 1 cut(s) 97
PkrI GCNGC 3 cut(s) 214, 238, 274
Psp6I CCWGG 2 cut(s) 80, 165
PspGI CCWGG 2 cut(s) 80, 165
PspN4I GGNNCC 3 cut(s) 65, 109, 148
PspPI GGNCC 5 cut(s) 64, 147, 157, 199, 226
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 2 cut(s) 44, 299
RsaNI GTAC 2 cut(s) 43, 298
SaqAI TTAA 3 cut(s) 89, 134, 140
SatI GCNGC 3 cut(s) 213, 237, 273
Sau3AI GATC 2 cut(s) 37, 162
Sau96I GGNCC 5 cut(s) 64, 147, 157, 199, 226
ScaI AGTACT 1 cut(s) 44
ScrFI CCNGG 4 cut(s) 82, 145, 167, 209
SetI ASST 5 cut(s) 36, 86, 241, 293, 321
SexAI ACCWGGT 1 cut(s) 80
SgrAI CRCCGGYG 1 cut(s) 95
SinI GGWCC 3 cut(s) 147, 199, 226
Sse9I AATT 2 cut(s) 102, 365
SsiI CCGC 4 cut(s) 93, 129, 212, 258
SspMI CTAG 4 cut(s) 72, 182, 203, 230
StyD4I CCNGG 4 cut(s) 80, 143, 165, 207
TaaI ACNGT 1 cut(s) 151
TaiI ACGT 3 cut(s) 36, 293, 321
TaqI TCGA 1 cut(s) 36
TasI AATT 2 cut(s) 102, 365
TatI WGTACW 2 cut(s) 42, 297
TauI GCSGC 1 cut(s) 215
Tru1I TTAA 3 cut(s) 89, 134, 140
Tru9I TTAA 3 cut(s) 89, 134, 140
TseI GCWGC 2 cut(s) 236, 272
TspDTI ATGAA 1 cut(s) 8
TspGWI ACGGA 1 cut(s) 42
VpaK11BI GGWCC 3 cut(s) 147, 199, 226
XspI CTAG 4 cut(s) 72, 182, 203, 230
ZraI GACGTC 1 cut(s) 34
ZrmI AGTACT 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.