FvH4_5g18140

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011260
Physical Location & Seq
Forward (+)
10423569 .. 10427109
3541 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18140.t1

Sequence Viewer

Length: 2550 bp
ATGGTTTCGCTTTTGTTTTTCCAGGCCTCCACTTTTATCCCTTTCATGTCTTTAGCTTACAGTGATTTCTACCAGATCTTGCTAATTGGTTGCTATTTTGCTAAAGTGCTCGGAAAAAGAAAGATGTTACGGTGCGCTACACAACTCACTCGTCGCCAACTTCTTCACCACTTCACAACTCTACGCGCCACAACTACACAAACCTACTCCTCTAACTACATCAATCTTCTTGAAGCCTGCATTCGATCCAAATCTCTCGCTCAAGCCAAGACAATCCACCAACACCTCATAAAAACCAAAACCCACATCGAGTCGAGGTCTCTTCTTCTTGATAAACTGGCCCGTCTCTACTTAACCTGTAATCAACTCGACGTCGCACGCCGACTGTTCGATGAAATTCCCCAACCAAATGCTATACTATGCAACCTCTTTATACGAGCCTACGCGTGGAATGGACCCTTTGAACGAGCCATTGAGTTGTACTACGCCATGCTCGAATCGGGTGTTGAACCGACCAAGTACACGTACCCTTTTGTTCTCAAGGCATGCTCTGCTTTGCAAGCACTTCAAGTTGGTGGGGATATTCATCGACACGCGAAACGACTCGGGCTCGACTCTGACGTATATGTTTGCACTGCTCTGATTGATTTGTATGCGAAATGTGGTGAGGTGGTGGCTGCAGAGAGTGTGTTTGATGGGATGTTGTGTAAAGATGTTGTGGCGTGGAATGCTATGATTGCCGGGTTTTCGCTTCATGGTCTTTATGATGACACTGTAAGGTTGGTTGTTAGGATGCAGGGAGCGGGAACAGCCCTGAATGCTTCTACTATTGTTACAGTTCTTCCTACTGTTGCGCAGGCTAATGCGTTGAGCCAAGGGAAGGCGATACATGGTTACTCTATGAGGAGGAGGTTTAGTGATGATGTGGTACTTAGTACTGCGCTTTTGGACATGTATGCGAAATGTCAGTGCATAGTTTACGCGAGGAGAATATTTGATGCAATGAGTGTTAGGAATGAGGTTTGTTGGAGTGCAATGATAGGTGCATATGTTGGGTGTGACTCCATGAGGGAGGCAATGTCATTGTTTGATGAAATGGTGGTGAAAGATATGAGGAAAGTGACACGAGTTACTCTAGGAAGCATACTCCGAGCTTGCACAAAGCTGATTGATCTGAGTGGAGGGAGACGAGTACATTGTTATGCAATTAAATCCGGTTTTGAATTAAGTACAATGGTGGGCAACACCATCCTTTCTATGTATGCAAAGTGTGGGATTATAGATGATGCATTCAGGTTATTTGATATAATGGAGCCAAAAGATACGGTTTCTTATGGTGCTATCATTTCAGGCTGTGTGCAGAATGGTTATGCAAAGGAAGCTTTGCTTATATTCCACCAGATGCAGTTGTCTGGGATTGATCCAGATTTGGCAACCATGGCAGGTGTTTTGCCTGCCTGTTCACATTTGGCTGCTCTGCAACATGGGGCCTGTGGCCATGGTTACTCAATTGTTCGCGGCTTTGTTAATGACACCTCAATATGTAATGCCTTAATAGACATGTACTCGAAATGTGGGAAGATCCATATAGGCAGGCAAGTATTCGACAGAATGCTTAAGCGGGACACTATCTCATGGAATGCAATGATAGTTGGTTATGGGATTCATGGCCTTGGGATAAAAGCAATTTCACAGTTCCACAGTATGCAGGCAGCTGGAATGAAGCCGGATGATGTAACTTTCATTGGCCTCTTATCTGCTTGCAGCCATTCGGGGCTTGTCACTGAAGGAAAACACTGGTTTAGTTCTATGACTCGAGATTTCAGCATAATCCCAAGGATGGAACACTACATATGCATGGTTGATCTTCTTGCCCGAACTGGACTGTTGGCCGAGGCACACAATTTCATTCAAGACATGCCATTTGAGGCTGATGTTCGAGTATGGAGTGCACTGCTTGCTGCATGTAGGGTTCACAATGATATTGAACTTGGGGAAGAAGTGTCCAGGAAAGTTCAAGGGCTAGGATCTGAAGGTACTGGAAATTTGGTCCTCTTATCTAATATGTACAGTTCTGTTGGGAGATGGGATGACGCAGCGCATGTGAGAATTAAACAGAAGGATCAAGGCCTTAAGAAGAGTCCAGGATGTAGTTGGGTTGAAATTAATGGGGTGATTCATGGATTTCTCGGTGGAGATCAGTCACATCCAGAATCGGCTCAGATACATGAAAAATTAGAGGAACTACTAGTAGACATGAAAGAGATGGGATATTGTGCAGAAAATAGTTTTGTTCTCCAAGATGTTGAGGAAGAGGAGAAAGAAAGGATCCTCCTTTACCATAGTGAGAAACTAGCCATTGCTTATGGAATTCTTCATCTGAGCCAGGGGAAACCCATTTTTGTTACTAAAAATCTGCGGGTCTGTGGTGATTGCCATGCTGCTATAAAGTTCATCTCTCTTGTTACAAAGAGAGAGATAAGCATAAGAGATGTGAGTCGGTTTCATCATTTCAGGGATGGAAATTGTAGTTGTGCAGACTTTTGGTGA

Protein Analysis

850

Amino Acids

94.58

Weight (kDa)

8.0

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 136 - 183 1.1e-08 PPR repeat family
PPR_2 PF13041 440 - 487 1.9e-07 PPR repeat family
PPR PF01535 443 - 473 8.1e-06 PPR repeat
PPR_2 PF13041 542 - 589 1.1e-07 PPR repeat family
E_motif PF20431 659 - 721 6.6e-18 E motif
Eplus_motif PF20430 726 - 750 2.4e-07 E+ motif
DYW_deaminase PF14432 757 - 849 5.6e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1433
AatII GACGTC 1 cut(s) 375
Acc16I TGCGCA 1 cut(s) 855
Acc36I ACCTGC 1 cut(s) 1433
AccBSI CCGCTC 1 cut(s) 803
AccI GTMKAC 1 cut(s) 2253
AccII CGCG 5 cut(s) 186, 446, 596, 983, 1518
AciI CCGC 4 cut(s) 803, 1518, 1621, 2419
AclWI GGATC 7 cut(s) 240, 1415, 1576, 2035, 2130, 2323, 2336
AcoI YGGCCR 2 cut(s) 1495, 1890
AcsI RAATTY 3 cut(s) 396, 2044, 2370
AcuI CTGAAG 2 cut(s) 1806, 2052
AcyI GRCGYC 1 cut(s) 372
AfiI CCNNNNNNNGG 3 cut(s) 447, 880, 1840
AflII CTTAAG 2 cut(s) 1616, 2132
AflIII ACRYGT 4 cut(s) 444, 522, 951, 1560
AgsI TTSAA 9 cut(s) 233, 464, 509, 569, 1223, 1913, 1988, 2018, 2162
AhlI ACTAGT 1 cut(s) 2248
AjnI CCWGG 4 cut(s) 21, 2006, 2143, 2385
AluBI AGCT 5 cut(s) 56, 1154, 1165, 1382, 1715
AluI AGCT 5 cut(s) 56, 1154, 1165, 1382, 1715
Alw21I GWGCWC 2 cut(s) 111, 1954
Alw26I GTCTC 3 cut(s) 324, 350, 1180
Alw44I GTGCAC 1 cut(s) 1950
AlwI GGATC 7 cut(s) 240, 1415, 1576, 2035, 2130, 2323, 2336
Ama87I CYCGRG 2 cut(s) 605, 1815
AoxI GGCC 8 cut(s) 24, 339, 1488, 1495, 1669, 1747, 1890, 2128
ApaLI GTGCAC 1 cut(s) 1950
ApeKI GCWGC 7 cut(s) 677, 1472, 1712, 1764, 1961, 2096, 2441
ApoI RAATTY 3 cut(s) 396, 2044, 2370
ArsI GACNNNNNNTTYG 2 cut(s) 1907, 1939
AseI ATTAAT 1 cut(s) 2166
AspLEI GCGC 5 cut(s) 137, 188, 856, 943, 2101
AspS9I GGNCC 4 cut(s) 340, 455, 1488, 2050
AsuC2I CCSGG 1 cut(s) 742
AsuHPI GGTGA 5 cut(s) 158, 677, 1114, 2185, 2441
AvaI CYCGRG 2 cut(s) 605, 1815
AvaII GGWCC 2 cut(s) 455, 2050
BaeGI GKGCMC 1 cut(s) 1954
BalI TGGCCA 1 cut(s) 1497
BamHI GGATCC 1 cut(s) 2328
BanII GRGCYC 1 cut(s) 612
BauI CACGAG 1 cut(s) 1125
Bbv12I GWGCWC 2 cut(s) 111, 1954
BbvI GCAGC 7 cut(s) 664, 1459, 1724, 1776, 1948, 2108, 2428
BccI CCATC 6 cut(s) 689, 1256, 1834, 2079, 2260, 2513
BciT130I CCWGG 4 cut(s) 23, 2008, 2145, 2387
BcnI CCSGG 1 cut(s) 742
BcoDI GTCTC 3 cut(s) 324, 350, 1180
BcuI ACTAGT 1 cut(s) 2248
BfaI CTAG 4 cut(s) 1136, 2024, 2249, 2354
BfmI CTRYAG 1 cut(s) 678
BfrI CTTAAG 2 cut(s) 1616, 2132
BfuAI ACCTGC 1 cut(s) 1433
BglII AGATCT 1 cut(s) 75
BisI GCNGC 8 cut(s) 678, 1473, 1519, 1713, 1765, 1962, 2097, 2442
BlsI GCNGC 8 cut(s) 679, 1474, 1520, 1714, 1766, 1963, 2098, 2443
BmcAI AGTACT 1 cut(s) 937
Bme1390I CCNGG 5 cut(s) 23, 742, 2008, 2145, 2387
Bme18I GGWCC 2 cut(s) 455, 2050
BmeT110I CYCGRG 2 cut(s) 605, 1815
BmgT120I GGNCC 4 cut(s) 340, 455, 1488, 2050
BmiI GGNNCC 4 cut(s) 457, 1314, 1489, 2330
BmrFI CCNGG 5 cut(s) 23, 742, 2008, 2145, 2387
BmsI GCATC 4 cut(s) 783, 988, 1276, 1392
BpuEI CTTGAG 2 cut(s) 246, 524
BpuMI CCSGG 1 cut(s) 742
BsaAI YACGTR 1 cut(s) 525
BsaBI GATNNNNATC 2 cut(s) 250, 585
BsaHI GRCGYC 1 cut(s) 372
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 7 cut(s) 874, 1437, 1498, 1672, 1835, 1893, 2386
BsaWI WCCGGW 1 cut(s) 1214
BsaXI ACNNNNNCTCC 4 cut(s) 901, 931, 1064, 1094
Bsc4I CCNNNNNNNGG 3 cut(s) 447, 880, 1840
Bse1I ACTGG 4 cut(s) 342, 1802, 1885, 2044
Bse3DI GCAATG 5 cut(s) 1008, 1041, 1083, 1650, 2358
Bse8I GATNNNNATC 2 cut(s) 250, 585
BseBI CCWGG 4 cut(s) 23, 2008, 2145, 2387
BseDI CCNNGG 7 cut(s) 874, 1437, 1498, 1672, 1835, 1893, 2386
BseGI GGATG 9 cut(s) 705, 798, 1248, 1735, 1845, 2095, 2153, 2206, 2524
BseJI GATNNNNATC 2 cut(s) 250, 585
BseLI CCNNNNNNNGG 3 cut(s) 447, 880, 1840
BseMI GCAATG 5 cut(s) 1008, 1041, 1083, 1650, 2358
BseMII CTCAG 3 cut(s) 1166, 2234, 2372
BseNI ACTGG 4 cut(s) 342, 1802, 1885, 2044
BseRI GAGGAG 5 cut(s) 199, 919, 922, 1000, 2330
BseSI GKGCMC 1 cut(s) 1954
BseXI GCAGC 7 cut(s) 664, 1459, 1724, 1776, 1948, 2108, 2428
BsgI GTGCAG 2 cut(s) 1379, 2298
Bsh1236I CGCG 5 cut(s) 186, 446, 596, 983, 1518
BshFI GGCC 8 cut(s) 26, 341, 1490, 1497, 1671, 1749, 1892, 2130
BsiHKAI GWGCWC 2 cut(s) 111, 1954
BsiHKCI CYCGRG 2 cut(s) 605, 1815
BsiSI CCGG 3 cut(s) 741, 1215, 1727
BslFI GGGAC 1 cut(s) 1637
BslI CCNNNNNNNGG 3 cut(s) 447, 880, 1840
BsmAI GTCTC 3 cut(s) 324, 350, 1180
BsmBI CGTCTC 2 cut(s) 350, 1180
BsmFI GGGAC 1 cut(s) 1637
BsmI GAATGC 6 cut(s) 240, 733, 823, 1289, 1617, 1645
BsnI GGCC 8 cut(s) 26, 341, 1490, 1497, 1671, 1749, 1892, 2130
Bso31I GGTCTC 1 cut(s) 324
BsoBI CYCGRG 2 cut(s) 605, 1815
Bsp1286I GDGCHC 3 cut(s) 111, 612, 1954
Bsp1407I TGTACA 1 cut(s) 2067
Bsp19I CCATGG 2 cut(s) 1437, 1498
BspACI CCGC 4 cut(s) 803, 1518, 1621, 2419
BspANI GGCC 8 cut(s) 26, 341, 1490, 1497, 1671, 1749, 1892, 2130
BspCNI CTCAG 3 cut(s) 1167, 2233, 2373
BspFNI CGCG 5 cut(s) 186, 446, 596, 983, 1518
BspLI GGNNCC 4 cut(s) 457, 1314, 1489, 2330
BspMAI CTGCAG 1 cut(s) 682
BspMI ACCTGC 1 cut(s) 1433
BspPI GGATC 7 cut(s) 240, 1415, 1576, 2035, 2130, 2323, 2336
BspTI CTTAAG 2 cut(s) 1616, 2132
BspTNI GGTCTC 1 cut(s) 324
BsrBI CCGCTC 1 cut(s) 803
BsrDI GCAATG 5 cut(s) 1008, 1041, 1083, 1650, 2358
BsrGI TGTACA 1 cut(s) 2067
BsrI ACTGG 4 cut(s) 342, 1802, 1885, 2044
BssECI CCNNGG 7 cut(s) 874, 1437, 1498, 1672, 1835, 1893, 2386
BssNI GRCGYC 1 cut(s) 372
BssSI CACGAG 1 cut(s) 1125
BssT1I CCWWGG 5 cut(s) 874, 1437, 1498, 1672, 1835
Bst2BI CACGAG 1 cut(s) 1125
Bst2UI CCWGG 4 cut(s) 23, 2008, 2145, 2387
Bst6I CTCTTC 3 cut(s) 327, 2132, 2307
BstACI GRCGYC 1 cut(s) 372
BstAFI CTTAAG 2 cut(s) 1616, 2132
BstAPI GCANNNNNTGC 2 cut(s) 551, 1958
BstAUI TGTACA 1 cut(s) 2067
BstBAI YACGTR 1 cut(s) 525
BstDEI CTNAG 4 cut(s) 932, 1175, 2220, 2381
BstDSI CCRYGG 2 cut(s) 1437, 1498
BstF5I GGATG 9 cut(s) 705, 798, 1248, 1735, 1845, 2095, 2153, 2206, 2524
BstFNI CGCG 5 cut(s) 186, 446, 596, 983, 1518
BstHHI GCGC 5 cut(s) 137, 188, 856, 943, 2101
BstMAI GTCTC 3 cut(s) 324, 350, 1180
BstMWI GCNNNNNNNGC 9 cut(s) 551, 560, 728, 737, 809, 818, 1379, 1439, 1958
BstNI CCWGG 4 cut(s) 23, 2008, 2145, 2387
BstNSI RCATGY 6 cut(s) 549, 955, 1564, 1921, 1968, 2105
BstSCI CCNGG 5 cut(s) 21, 740, 2006, 2143, 2385
BstSFI CTRYAG 1 cut(s) 678
BstSLI GKGCMC 1 cut(s) 1954
BstUI CGCG 5 cut(s) 186, 446, 596, 983, 1518
BstV1I GCAGC 7 cut(s) 664, 1459, 1724, 1776, 1948, 2108, 2428
BstX2I RGATCY 4 cut(s) 75, 1581, 2027, 2328
BstYI RGATCY 4 cut(s) 75, 1581, 2027, 2328
BsuRI GGCC 8 cut(s) 26, 341, 1490, 1497, 1671, 1749, 1892, 2130
BtgI CCRYGG 2 cut(s) 1437, 1498
BtsCI GGATG 9 cut(s) 705, 798, 1248, 1735, 1845, 2095, 2153, 2206, 2524
BtsI GCAGTG 2 cut(s) 633, 1952
BtsIMutI CAGTG 7 cut(s) 67, 633, 771, 974, 1782, 1795, 1952
BveI ACCTGC 1 cut(s) 1433
CfoI GCGC 5 cut(s) 137, 188, 856, 943, 2101
Cfr13I GGNCC 4 cut(s) 340, 455, 1488, 2050
CseI GACGC 1 cut(s) 2102
DdeI CTNAG 4 cut(s) 932, 1175, 2220, 2381
EaeI YGGCCR 2 cut(s) 1495, 1890
Eam1104I CTCTTC 3 cut(s) 327, 2132, 2307
EarI CTCTTC 3 cut(s) 327, 2132, 2307
Eco130I CCWWGG 5 cut(s) 874, 1437, 1498, 1672, 1835
Eco147I AGGCCT 2 cut(s) 26, 2130
Eco24I GRGCYC 1 cut(s) 612
Eco31I GGTCTC 1 cut(s) 324
Eco47I GGWCC 2 cut(s) 455, 2050
Eco57I CTGAAG 2 cut(s) 1806, 2052
Eco88I CYCGRG 2 cut(s) 605, 1815
EcoO109I RGGNCCY 1 cut(s) 1488
EcoRI GAATTC 1 cut(s) 2370
EcoRII CCWGG 4 cut(s) 21, 2006, 2143, 2385
EcoT14I CCWWGG 5 cut(s) 874, 1437, 1498, 1672, 1835
EcoT22I ATGCAT 2 cut(s) 1291, 1859
EcoT38I GRGCYC 1 cut(s) 612
ErhI CCWWGG 5 cut(s) 874, 1437, 1498, 1672, 1835
Esp3I CGTCTC 2 cut(s) 350, 1180
FalI AAGNNNNNCTT 2 cut(s) 1371, 1403
FaqI GGGAC 1 cut(s) 1637
FauI CCCGC 3 cut(s) 796, 1614, 2412
FauNDI CATATG 2 cut(s) 1048, 1853
FblI GTMKAC 1 cut(s) 2253
Fnu4HI GCNGC 8 cut(s) 678, 1473, 1519, 1713, 1765, 1962, 2097, 2442
FokI GGATG 9 cut(s) 712, 805, 1235, 1742, 1852, 2102, 2160, 2193, 2531
FriOI GRGCYC 1 cut(s) 612
Fsp4HI GCNGC 8 cut(s) 678, 1473, 1519, 1713, 1765, 1962, 2097, 2442
FspBI CTAG 4 cut(s) 1136, 2024, 2249, 2354
FspI TGCGCA 1 cut(s) 855
GlaI GCGC 5 cut(s) 136, 187, 855, 942, 2100
GluI GCNGC 8 cut(s) 678, 1473, 1519, 1713, 1765, 1962, 2097, 2442
HaeIII GGCC 8 cut(s) 26, 341, 1490, 1497, 1671, 1749, 1892, 2130
HapII CCGG 3 cut(s) 741, 1215, 1727
HgaI GACGC 1 cut(s) 2102
HhaI GCGC 5 cut(s) 137, 188, 856, 943, 2101
Hin1I GRCGYC 1 cut(s) 372
Hin6I GCGC 5 cut(s) 135, 186, 854, 941, 2099
HinP1I GCGC 5 cut(s) 135, 186, 854, 941, 2099
HindIII AAGCTT 1 cut(s) 1380
HpaII CCGG 3 cut(s) 741, 1215, 1727
HphI GGTGA 5 cut(s) 158, 677, 1114, 2185, 2441
Hpy166II GTNNAC 6 cut(s) 522, 979, 1463, 1952, 1975, 2254
Hpy188I TCNGA 8 cut(s) 113, 619, 642, 1151, 1176, 2032, 2223, 2382
Hpy188III TCNNGA 6 cut(s) 230, 329, 1424, 1817, 1913, 2210
Hpy8I GTNNAC 6 cut(s) 522, 979, 1463, 1952, 1975, 2254
Hpy99I CGWCG 3 cut(s) 156, 374, 377
HpyAV CCTTC 4 cut(s) 874, 1781, 2027, 2113
HpyCH4IV ACGT 3 cut(s) 372, 524, 621
HpyF10VI GCNNNNNNNGC 9 cut(s) 551, 560, 728, 737, 809, 818, 1379, 1439, 1958
HpyF3I CTNAG 4 cut(s) 932, 1175, 2220, 2381
HpySE526I ACGT 3 cut(s) 372, 524, 621
Hsp92I GRCGYC 1 cut(s) 372
HspAI GCGC 5 cut(s) 135, 186, 854, 941, 2099
LmnI GCTCC 2 cut(s) 800, 1312
Lsp1109I GCAGC 7 cut(s) 664, 1459, 1724, 1776, 1948, 2108, 2428
LweI GCATC 4 cut(s) 783, 988, 1276, 1392
MaeI CTAG 4 cut(s) 1136, 2024, 2249, 2354
MaeII ACGT 3 cut(s) 372, 524, 621
MbiI CCGCTC 1 cut(s) 803
MfeI CAATTG 1 cut(s) 1509
MflI RGATCY 4 cut(s) 75, 1581, 2027, 2328
MhlI GDGCHC 3 cut(s) 111, 612, 1954
MlsI TGGCCA 1 cut(s) 1497
MluI ACGCGT 1 cut(s) 444
MluNI TGGCCA 1 cut(s) 1497
MlyI GAGTC 7 cut(s) 320, 597, 608, 1055, 1807, 2149, 2506
MmeI TCCRAC 1 cut(s) 1007
Mox20I TGGCCA 1 cut(s) 1497
Mph1103I ATGCAT 2 cut(s) 1291, 1859
MscI TGGCCA 1 cut(s) 1497
MseI TTAA 9 cut(s) 353, 1209, 1226, 1527, 1553, 1617, 2112, 2133, 2166
MslI CAYNNNNRTG 2 cut(s) 1200, 1856
Msp20I TGGCCA 1 cut(s) 1497
MspA1I CMGCKG 1 cut(s) 1715
MspCI CTTAAG 2 cut(s) 1616, 2132
MspI CCGG 3 cut(s) 741, 1215, 1727
MspR9I CCNGG 5 cut(s) 23, 742, 2008, 2145, 2387
MunI CAATTG 1 cut(s) 1509
Mva1269I GAATGC 6 cut(s) 240, 733, 823, 1289, 1617, 1645
MvaI CCWGG 4 cut(s) 23, 2008, 2145, 2387
MvnI CGCG 5 cut(s) 186, 446, 596, 983, 1518
MwoI GCNNNNNNNGC 9 cut(s) 551, 560, 728, 737, 809, 818, 1379, 1439, 1958
NciI CCSGG 1 cut(s) 742
NcoI CCATGG 2 cut(s) 1437, 1498
NdeI CATATG 2 cut(s) 1048, 1853
NlaIV GGNNCC 4 cut(s) 457, 1314, 1489, 2330
NmeAIII GCCGAG 1 cut(s) 1918
NmuCI GTSAC 4 cut(s) 1058, 1120, 1780, 2202
NsbI TGCGCA 1 cut(s) 855
NsiI ATGCAT 2 cut(s) 1291, 1859
NspI RCATGY 6 cut(s) 549, 955, 1564, 1921, 1968, 2105
PaeI GCATGC 1 cut(s) 549
PaeR7I CTCGAG 1 cut(s) 1815
PaqCI CACCTGC 1 cut(s) 1433
PceI AGGCCT 2 cut(s) 26, 2130
PciI ACATGT 2 cut(s) 951, 1560
PcsI WCGNNNNNNNCGW 2 cut(s) 492, 618
PctI GAATGC 6 cut(s) 240, 733, 823, 1289, 1617, 1645
PfeI GAWTC 4 cut(s) 497, 1663, 2176, 2213
PfoI TCCNGGA 2 cut(s) 2006, 2143
PkrI GCNGC 8 cut(s) 679, 1474, 1520, 1714, 1766, 1963, 2098, 2443
PleI GAGTC 7 cut(s) 319, 597, 608, 1055, 1807, 2148, 2505
PpsI GAGTC 7 cut(s) 319, 597, 608, 1055, 1807, 2148, 2505
Ppu21I YACGTR 1 cut(s) 525
PscI ACATGT 2 cut(s) 951, 1560
PshBI ATTAAT 1 cut(s) 2166
Psp6I CCWGG 4 cut(s) 21, 2006, 2143, 2385
PspGI CCWGG 4 cut(s) 21, 2006, 2143, 2385
PspN4I GGNNCC 4 cut(s) 457, 1314, 1489, 2330
PspPI GGNCC 4 cut(s) 340, 455, 1488, 2050
PstI CTGCAG 1 cut(s) 682
PsuI RGATCY 4 cut(s) 75, 1581, 2027, 2328
PvuII CAGCTG 1 cut(s) 1715
RseI CAYNNNNRTG 2 cut(s) 1200, 1856
SaqAI TTAA 9 cut(s) 353, 1209, 1226, 1527, 1553, 1617, 2112, 2133, 2166
SatI GCNGC 8 cut(s) 678, 1473, 1519, 1713, 1765, 1962, 2097, 2442
Sau96I GGNCC 4 cut(s) 340, 455, 1488, 2050
ScaI AGTACT 1 cut(s) 937
SchI GAGTC 7 cut(s) 320, 597, 608, 1055, 1807, 2149, 2506
ScrFI CCNGG 5 cut(s) 23, 742, 2008, 2145, 2387
SduI GDGCHC 3 cut(s) 111, 612, 1954
SfaNI GCATC 4 cut(s) 783, 988, 1276, 1392
SfcI CTRYAG 1 cut(s) 678
Sfr274I CTCGAG 1 cut(s) 1815
SinI GGWCC 2 cut(s) 455, 2050
SlaI CTCGAG 1 cut(s) 1815
SmiMI CAYNNNNRTG 2 cut(s) 1200, 1856
SmlI CTYRAG 5 cut(s) 261, 539, 1616, 1815, 2132
SmoI CTYRAG 5 cut(s) 261, 539, 1616, 1815, 2132
SpeI ACTAGT 1 cut(s) 2248
SphI GCATGC 1 cut(s) 549
SseBI AGGCCT 2 cut(s) 26, 2130
SsiI CCGC 4 cut(s) 803, 1518, 1621, 2419
SspI AATATT 1 cut(s) 993
SspMI CTAG 4 cut(s) 1136, 2024, 2249, 2354
StuI AGGCCT 2 cut(s) 26, 2130
StyD4I CCNGG 5 cut(s) 21, 740, 2006, 2143, 2385
StyI CCWWGG 5 cut(s) 874, 1437, 1498, 1672, 1835
TaiI ACGT 3 cut(s) 375, 527, 624
TatI WGTACW 7 cut(s) 480, 519, 935, 1192, 1229, 1563, 2067
TauI GCSGC 1 cut(s) 1521
TfiI GAWTC 4 cut(s) 497, 1663, 2176, 2213
Tru1I TTAA 9 cut(s) 353, 1209, 1226, 1527, 1553, 1617, 2112, 2133, 2166
Tru9I TTAA 9 cut(s) 353, 1209, 1226, 1527, 1553, 1617, 2112, 2133, 2166
TscAI CASTG 7 cut(s) 67, 640, 778, 974, 1789, 1802, 1959
TseFI GTSAC 4 cut(s) 1058, 1120, 1780, 2202
TseI GCWGC 7 cut(s) 677, 1472, 1712, 1764, 1961, 2096, 2441
Tsp45I GTSAC 4 cut(s) 1058, 1120, 1780, 2202
TspRI CASTG 7 cut(s) 67, 640, 778, 974, 1789, 1802, 1959
Vha464I CTTAAG 2 cut(s) 1616, 2132
VneI GTGCAC 1 cut(s) 1950
VpaK11BI GGWCC 2 cut(s) 455, 2050
VspI ATTAAT 1 cut(s) 2166
XapI RAATTY 3 cut(s) 396, 2044, 2370
XceI RCATGY 6 cut(s) 549, 955, 1564, 1921, 1968, 2105
XcmI CCANNNNNNNNNTGG 1 cut(s) 2151
XhoI CTCGAG 1 cut(s) 1815
XmiI GTMKAC 1 cut(s) 2253
XspI CTAG 4 cut(s) 1136, 2024, 2249, 2354
ZraI GACGTC 1 cut(s) 373
ZrmI AGTACT 1 cut(s) 937
Zsp2I ATGCAT 2 cut(s) 1291, 1859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.