pycom14g20490

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011260
Physical Location & Seq
Forward (+)
21542947 .. 21545412
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g20490.1

Sequence Viewer

Length: 2466 bp
ATGTTATGTTTTGGTAGGCGATCACTCTCTCGGAGTCGGAGTCGGAGTCAATACTATCATACCACTCAGCAAGCCATAGTGATATTAACCCACAACTTTTCGACAAACACCCTCACTCGTCTCGACTTTGATTACAAACACCTTCTCGAAGCCTGCATTCGATCCAAATCTCTTCCTCAAGGCAAGAAAATCCATCAGCTCCTCCTGAAAAACACTACCCACCTCAAAGATAGCTCCTTTTTGCTTGAAAAAGTAGCCCATTTGTACATAACCTGCAATCAACTCCAGCTTGCATGCCGCGTGTTCGATAAAATTCCCCAACCAAGTGTTATTCTTTGGAACCTTTTGATAAGAGGATACGCTTGGAACGGGCCCCTTGATAAAGCCATCGACTTGTACTATGGCATGCTCAAATCCGGCGTTCAACCCACCAACTATACTTACCCTTTTGTTCTCAAGGCTTGCTCCGGTTTACAAGCACTCGAAGTTGGCCGAAAGATCCATGAGCATGCCAGAGGACTTGGGCTTCACTCTGATGTCTACGTTTGCACTGCTTTGATCGACCTGTATGCCAAGTGTGGGGACTTGGTGGAGGCTCAGAGGGTGTTCCATGGCATGCTGTACAAAGACATTGTGGCGTGGAATGCAATGATTGCTGGGTTTTCACTACACGGTCTTTATGATGACACTGTACAGTTGCTTGTTCAAATGCAGCGAGCGGGAACAAGCCCAAACTCTTCCACCATTGTTTCGGTTCTTCCCACTGTTGCACAAGCTAATGCATTGAGGCAGGGGAAGGCAATGCATGGTTACTCCCTAAGAAGGGGCTTTAGTGGTGAGGTGGTGCTTGGTACTGGCCTTTTGGATTTGTATTCTAAATGCCAGTGCATAGCTTATGCGAGGAGAATCTTTGATGCCATGAATTTCAAGAACGAGGTTTGTTGGAGTGCTATGATAGGTGCATATGTTATCTGTGACTCTATGACAGAGGCGATAGCATTGTTTGATGAAATGGTTCTACGAGAGGAAATAAATCCAACAGCAGTAACTCTCGGGAGCATTCTACGAGCTTGCGCAAAGCTAACTGATCTGAGTAGAGGGAGACGGGTTCATTGCTATGCAATTAAAGCTGGGTTTGATTTAGATAAAATGGTGGGTAACACAATGCTGTCTGTGTATGCAAAGTGTGGGATTAATGATGATGCAGTCAGATTCTTTGATACAATGAACTCCAAAGATACAGTTTCTTATGGTGCGATCATCTCAGGCTGTGTGCAGAATGGTTATGCGAAGGAAGCTTTACTTATGTTCCATAATATGCAATTGTCTGGGATCAATCCAGATGTGGCAACCATGAAGGGTGTTCTGCCTGCTTGTTCACATTTGGCCGCTCTACAGCACGGGGCCTGTGGACATGCTTATTCAATTGTTCACGGATTTTGTACAGACACCTCAATCTGTAATGCCTTAATAGACATGTACTCAAAGTGTGGGAAGATCAATGTAGGAAGGCAAGTTTTTGATAAAATGGTTAAGCGTGATATTATTTCATGGAATGCAATGATAGTTGGTTATGGGACTCATGGCCTTGGGATGGAAGCAATCTCACAGTTCCATCATATGCAGTCATCTGGCATTAAGCCCGATGATGTGACTTTCATTGGCCTCTTATCTGCTTGCAGCCATTCGGGGCTTGTAACTGAAGGCAAACACTGGTTTAGTAAAATGAGTCAGGAGTTCAACATAAACCCGAGGATGGAACATTACATATGCATGGTGGATATTCTCGCCCGGGCAGGACTTTTAGATGAGGCACACGGTTTCATTCAAAGAATGCCGTTTCAGGCTGATGTTCGTGTGTGGAGTGCACTGCTTGCTGCATGCAGAGTCCACAATAATATGGAACTCGGGGAAGAAGTATCCAAGAAAATTCAAGGGAAAGGACTTGAAGGTACTGGGAATTTGGTCCTTTTATCTAACTTATACAGTGCTGTTGGGAGGTGGGATGATGCAGCCGATGTCAGAATCAAGCAGAAGGACCAAGGCCTTAAGAAGAGTCCAGGATGCAGTTGGGTTGAGATCGATGGTGTTATCCATGGATTTGTCGGTGGAGATCAGTCCCATCCTCAGTTAGCTCAGATTCATGAAAAACTAGAAGAACTACTAGTAGATATGAAACAGCTGGGATATCGTGTGGAAAATAGATTTGTTCTCCAAGATGTTGAGGAAGAGGAGAAAGAAAGAATCCTCCTTTACCACAGTGAGAAACTAGCCATTGCCTATGCAATTCTTTGTCTGAGCCCGGGCAAATCCATCTTAGTTACCAAGAATTTGCGGGTTTGTGGTGATTGCCATGCTGCCATAAAGTTCATCACTCTTGTTACAAAGAGAGAAATAACTGTAAGAGATGTGAGTCGGTTTCATCATTTCAAGGATGGAATTTGCAGTTGTGCAGACTTCTGGTGA

Protein Analysis

822

Amino Acids

91.49

Weight (kDa)

8.43

Isoelectric Point (pI)

38.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 108 - 155 3.7e-11 PPR repeat family
PPR PF01535 112 - 141 7.4e-06 PPR repeat
PPR_2 PF13041 209 - 251 4e-07 PPR repeat family
PPR_2 PF13041 311 - 360 7.7e-07 PPR repeat family
PPR_2 PF13041 412 - 460 2.9e-08 PPR repeat family
PPR_2 PF13041 513 - 561 4e-10 PPR repeat family
E_motif PF20431 631 - 693 8.2e-16 E motif
DYW_deaminase PF14432 729 - 821 1.6e-38 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1075
Acc36I ACCTGC 1 cut(s) 281
AccBSI CCGCTC 2 cut(s) 719, 1391
AccI GTMKAC 1 cut(s) 540
AccII CGCG 1 cut(s) 300
AciI CCGC 4 cut(s) 298, 719, 1389, 2335
AclWI GGATC 3 cut(s) 156, 493, 1340
AcoI YGGCCR 2 cut(s) 490, 1386
AcsI RAATTY 6 cut(s) 312, 922, 1929, 1960, 2329, 2439
AcuI CTGAAG 1 cut(s) 1722
AfaI GTAC 8 cut(s) 266, 398, 623, 693, 853, 1444, 1481, 1954
AfiI CCNNNNNNNGG 5 cut(s) 579, 822, 823, 1594, 1756
AflII CTTAAG 1 cut(s) 2048
AflIII ACRYGT 1 cut(s) 1476
AhlI ACTAGT 1 cut(s) 2164
AjnI CCWGG 1 cut(s) 2059
Alw21I GWGCWC 1 cut(s) 1870
Alw26I GTCTC 2 cut(s) 125, 1096
Alw44I GTGCAC 1 cut(s) 1866
AlwI GGATC 3 cut(s) 156, 493, 1340
Ama87I CYCGRG 5 cut(s) 1052, 1750, 1791, 1907, 2302
AoxI GGCC 8 cut(s) 371, 490, 856, 1386, 1404, 1585, 1663, 2044
ApaI GGGCCC 1 cut(s) 375
ApaLI GTGCAC 1 cut(s) 1866
ApeKI GCWGC 5 cut(s) 712, 1680, 1877, 2012, 2357
ApoI RAATTY 6 cut(s) 312, 922, 1929, 1960, 2329, 2439
AseI ATTAAT 1 cut(s) 1194
Asp700I GAANNNNTTC 1 cut(s) 1014
AspLEI GCGC 1 cut(s) 1076
AspS9I GGNCC 5 cut(s) 371, 372, 1404, 1966, 2038
AsuC2I CCSGG 4 cut(s) 1792, 1793, 2303, 2304
AsuHPI GGTGA 2 cut(s) 848, 2357
AvaI CYCGRG 5 cut(s) 1052, 1750, 1791, 1907, 2302
AvaII GGWCC 2 cut(s) 1966, 2038
BaeGI GKGCMC 2 cut(s) 375, 1870
BanII GRGCYC 2 cut(s) 375, 2303
Bbv12I GWGCWC 1 cut(s) 1870
BbvI GCAGC 5 cut(s) 724, 1692, 1864, 2024, 2344
BccI CCATC 9 cut(s) 201, 395, 1588, 1623, 1750, 2078, 2130, 2321, 2429
BceAI ACGGC 1 cut(s) 1822
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BciT130I CCWGG 1 cut(s) 2061
BciVI GTATCC 2 cut(s) 350, 1930
BcnI CCSGG 4 cut(s) 1792, 1793, 2303, 2304
BcoDI GTCTC 2 cut(s) 125, 1096
BcuI ACTAGT 1 cut(s) 2164
BfaI CTAG 3 cut(s) 2153, 2165, 2270
BfmI CTRYAG 1 cut(s) 1394
BfrI CTTAAG 1 cut(s) 2048
BfuAI ACCTGC 1 cut(s) 281
BfuI GTATCC 2 cut(s) 350, 1930
BisI GCNGC 7 cut(s) 298, 713, 1389, 1681, 1878, 2013, 2358
BlsI GCNGC 7 cut(s) 299, 714, 1390, 1682, 1879, 2014, 2359
Bme1390I CCNGG 5 cut(s) 1792, 1793, 2061, 2303, 2304
Bme18I GGWCC 2 cut(s) 1966, 2038
BmeT110I CYCGRG 5 cut(s) 1052, 1750, 1791, 1907, 2302
BmgT120I GGNCC 5 cut(s) 371, 372, 1404, 1966, 2038
BmiI GGNNCC 4 cut(s) 341, 373, 374, 1405
BmrFI CCNGG 5 cut(s) 1792, 1793, 2061, 2303, 2304
BmrI ACTGGG 1 cut(s) 1965
BmsI GCATC 4 cut(s) 904, 1192, 1999, 2054
BmuI ACTGGG 1 cut(s) 1965
BpmI CTGGAG 1 cut(s) 269
BpuEI CTTGAG 2 cut(s) 162, 440
BpuMI CCSGG 4 cut(s) 1792, 1793, 2303, 2304
Bsa29I ATCGAT 1 cut(s) 2082
BsaBI GATNNNNATC 1 cut(s) 166
BsaJI CCNNGG 7 cut(s) 610, 1588, 1751, 1791, 2041, 2095, 2302
BsaWI WCCGGW 1 cut(s) 467
BsaXI ACNNNNNCTCC 4 cut(s) 25, 31, 55, 61
Bsc4I CCNNNNNNNGG 5 cut(s) 579, 822, 823, 1594, 1756
Bse1I ACTGG 4 cut(s) 859, 883, 1718, 1960
Bse3DI GCAATG 5 cut(s) 654, 807, 1111, 1566, 2274
Bse8I GATNNNNATC 1 cut(s) 166
BseBI CCWGG 1 cut(s) 2061
BseCI ATCGAT 1 cut(s) 2082
BseDI CCNNGG 7 cut(s) 610, 1588, 1751, 1791, 2041, 2095, 2302
BseGI GGATG 6 cut(s) 1599, 1761, 2011, 2069, 2122, 2440
BseJI GATNNNNATC 1 cut(s) 166
BseLI CCNNNNNNNGG 5 cut(s) 579, 822, 823, 1594, 1756
BseMI GCAATG 5 cut(s) 654, 807, 1111, 1566, 2274
BseMII CTCAG 7 cut(s) 80, 611, 1082, 1278, 2141, 2150, 2288
BseNI ACTGG 4 cut(s) 859, 883, 1718, 1960
BseRI GAGGAG 3 cut(s) 191, 916, 2246
BseSI GKGCMC 2 cut(s) 375, 1870
BseXI GCAGC 5 cut(s) 724, 1692, 1864, 2024, 2344
BseYI CCCAGC 3 cut(s) 656, 1130, 2182
BsgI GTGCAG 1 cut(s) 1295
Bsh1236I CGCG 1 cut(s) 300
BshFI GGCC 8 cut(s) 373, 492, 858, 1388, 1406, 1587, 1665, 2046
BshVI ATCGAT 1 cut(s) 2082
BsiHKAI GWGCWC 1 cut(s) 1870
BsiHKCI CYCGRG 5 cut(s) 1052, 1750, 1791, 1907, 2302
BsiSI CCGG 4 cut(s) 417, 468, 1792, 2303
BslFI GGGAC 3 cut(s) 596, 1591, 2104
BslI CCNNNNNNNGG 5 cut(s) 579, 822, 823, 1594, 1756
BsmAI GTCTC 2 cut(s) 125, 1096
BsmBI CGTCTC 2 cut(s) 125, 1096
BsmFI GGGAC 3 cut(s) 596, 1591, 2104
BsmI GAATGC 5 cut(s) 156, 649, 1059, 1561, 1839
BsnI GGCC 8 cut(s) 373, 492, 858, 1388, 1406, 1587, 1665, 2046
BsoBI CYCGRG 5 cut(s) 1052, 1750, 1791, 1907, 2302
Bsp120I GGGCCC 1 cut(s) 371
Bsp1286I GDGCHC 3 cut(s) 375, 1870, 2303
Bsp1407I TGTACA 4 cut(s) 264, 621, 691, 1442
Bsp19I CCATGG 2 cut(s) 610, 2095
BspACI CCGC 4 cut(s) 298, 719, 1389, 2335
BspANI GGCC 8 cut(s) 373, 492, 858, 1388, 1406, 1587, 1665, 2046
BspCNI CTCAG 7 cut(s) 79, 610, 1083, 1277, 2140, 2149, 2289
BspDI ATCGAT 1 cut(s) 2082
BspFNI CGCG 1 cut(s) 300
BspHI TCATGA 1 cut(s) 2143
BspLI GGNNCC 4 cut(s) 341, 373, 374, 1405
BspMI ACCTGC 1 cut(s) 281
BspPI GGATC 3 cut(s) 156, 493, 1340
BspTI CTTAAG 1 cut(s) 2048
BsrBI CCGCTC 2 cut(s) 719, 1391
BsrDI GCAATG 5 cut(s) 654, 807, 1111, 1566, 2274
BsrGI TGTACA 4 cut(s) 264, 621, 691, 1442
BsrI ACTGG 4 cut(s) 859, 883, 1718, 1960
BssECI CCNNGG 7 cut(s) 610, 1588, 1751, 1791, 2041, 2095, 2302
BssT1I CCWWGG 4 cut(s) 610, 1588, 2041, 2095
Bst2UI CCWGG 1 cut(s) 2061
Bst6I CTCTTC 4 cut(s) 177, 742, 2048, 2223
BstAFI CTTAAG 1 cut(s) 2048
BstAPI GCANNNNNTGC 2 cut(s) 653, 1874
BstAUI TGTACA 4 cut(s) 264, 621, 691, 1442
BstDEI CTNAG 9 cut(s) 66, 597, 818, 1091, 1264, 2127, 2136, 2297, 2317
BstDSI CCRYGG 2 cut(s) 610, 2095
BstF5I GGATG 6 cut(s) 1599, 1761, 2011, 2069, 2122, 2440
BstFNI CGCG 1 cut(s) 300
BstHHI GCGC 1 cut(s) 1076
BstMAI GTCTC 2 cut(s) 125, 1096
BstMWI GCNNNNNNNGC 5 cut(s) 644, 653, 1127, 1295, 1874
BstNI CCWGG 1 cut(s) 2061
BstNSI RCATGY 7 cut(s) 297, 409, 512, 619, 1418, 1480, 1884
BstSCI CCNGG 5 cut(s) 1790, 1791, 2059, 2301, 2302
BstSFI CTRYAG 1 cut(s) 1394
BstSLI GKGCMC 2 cut(s) 375, 1870
BstUI CGCG 1 cut(s) 300
BstV1I GCAGC 5 cut(s) 724, 1692, 1864, 2024, 2344
BstX2I RGATCY 1 cut(s) 498
BstYI RGATCY 1 cut(s) 498
Bsu15I ATCGAT 1 cut(s) 2082
BsuI GTATCC 2 cut(s) 350, 1930
BsuRI GGCC 8 cut(s) 373, 492, 858, 1388, 1406, 1587, 1665, 2046
BsuTUI ATCGAT 1 cut(s) 2082
BtgI CCRYGG 2 cut(s) 610, 2095
BtsCI GGATG 6 cut(s) 1599, 1761, 2011, 2069, 2122, 2440
BtsI GCAGTG 2 cut(s) 549, 1868
BtsIMutI CAGTG 8 cut(s) 549, 687, 762, 890, 1711, 1868, 1993, 2266
BveI ACCTGC 1 cut(s) 281
CciI TCATGA 1 cut(s) 2143
CfoI GCGC 1 cut(s) 1076
Cfr13I GGNCC 5 cut(s) 371, 372, 1404, 1966, 2038
Cfr9I CCCGGG 2 cut(s) 1791, 2302
ClaI ATCGAT 1 cut(s) 2082
Csp6I GTAC 8 cut(s) 265, 397, 622, 692, 852, 1443, 1480, 1953
CviQI GTAC 8 cut(s) 265, 397, 622, 692, 852, 1443, 1480, 1953
DdeI CTNAG 9 cut(s) 66, 597, 818, 1091, 1264, 2127, 2136, 2297, 2317
EaeI YGGCCR 2 cut(s) 490, 1386
Eam1104I CTCTTC 4 cut(s) 177, 742, 2048, 2223
EarI CTCTTC 4 cut(s) 177, 742, 2048, 2223
Eco130I CCWWGG 4 cut(s) 610, 1588, 2041, 2095
Eco147I AGGCCT 1 cut(s) 2046
Eco24I GRGCYC 2 cut(s) 375, 2303
Eco32I GATATC 1 cut(s) 2189
Eco47I GGWCC 2 cut(s) 1966, 2038
Eco57I CTGAAG 1 cut(s) 1722
Eco88I CYCGRG 5 cut(s) 1052, 1750, 1791, 1907, 2302
EcoO109I RGGNCCY 2 cut(s) 372, 1404
EcoRII CCWGG 1 cut(s) 2059
EcoRV GATATC 1 cut(s) 2189
EcoT14I CCWWGG 4 cut(s) 610, 1588, 2041, 2095
EcoT22I ATGCAT 3 cut(s) 784, 807, 1775
EcoT38I GRGCYC 2 cut(s) 375, 2303
ErhI CCWWGG 4 cut(s) 610, 1588, 2041, 2095
Esp3I CGTCTC 2 cut(s) 125, 1096
FalI AAGNNNNNCTT 2 cut(s) 1287, 1319
FaqI GGGAC 3 cut(s) 596, 1591, 2104
FauI CCCGC 2 cut(s) 712, 2328
FauNDI CATATG 3 cut(s) 964, 1620, 1769
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 7 cut(s) 298, 713, 1389, 1681, 1878, 2013, 2358
FokI GGATG 6 cut(s) 1606, 1768, 2018, 2076, 2109, 2447
FriOI GRGCYC 2 cut(s) 375, 2303
Fsp4HI GCNGC 7 cut(s) 298, 713, 1389, 1681, 1878, 2013, 2358
FspBI CTAG 3 cut(s) 2153, 2165, 2270
FspI TGCGCA 1 cut(s) 1075
GlaI GCGC 1 cut(s) 1075
GluI GCNGC 7 cut(s) 298, 713, 1389, 1681, 1878, 2013, 2358
GsaI CCCAGC 3 cut(s) 660, 1134, 2186
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 8 cut(s) 373, 492, 858, 1388, 1406, 1587, 1665, 2046
HapII CCGG 4 cut(s) 417, 468, 1792, 2303
HhaI GCGC 1 cut(s) 1076
Hin6I GCGC 1 cut(s) 1074
HinP1I GCGC 1 cut(s) 1074
HindIII AAGCTT 1 cut(s) 1296
HpaII CCGG 4 cut(s) 417, 468, 1792, 2303
HphI GGTGA 2 cut(s) 848, 2357
Hpy166II GTNNAC 7 cut(s) 473, 541, 1379, 1412, 1432, 1868, 1891
Hpy188III TCNNGA 8 cut(s) 122, 146, 205, 928, 1054, 1340, 1733, 2144
Hpy8I GTNNAC 7 cut(s) 473, 541, 1379, 1412, 1432, 1868, 1891
HpyAV CCTTC 9 cut(s) 152, 790, 816, 1285, 1351, 1503, 1697, 1943, 2029
HpyCH4IV ACGT 1 cut(s) 543
HpyF10VI GCNNNNNNNGC 5 cut(s) 644, 653, 1127, 1295, 1874
HpyF3I CTNAG 9 cut(s) 66, 597, 818, 1091, 1264, 2127, 2136, 2297, 2317
HpySE526I ACGT 1 cut(s) 543
HspAI GCGC 1 cut(s) 1074
LmnI GCTCC 4 cut(s) 204, 239, 470, 1056
Lsp1109I GCAGC 5 cut(s) 724, 1692, 1864, 2024, 2344
LweI GCATC 4 cut(s) 904, 1192, 1999, 2054
MaeI CTAG 3 cut(s) 2153, 2165, 2270
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 8 cut(s) 809, 974, 1045, 1157, 1651, 1696, 2320, 2380
MbiI CCGCTC 2 cut(s) 719, 1391
MboII GAAGA 8 cut(s) 164, 729, 749, 1507, 1925, 2065, 2168, 2240
MfeI CAATTG 2 cut(s) 1322, 1425
MflI RGATCY 1 cut(s) 498
MhlI GDGCHC 3 cut(s) 375, 1870, 2303
MlyI GAGTC 9 cut(s) 43, 49, 55, 971, 1573, 1738, 1896, 2065, 2422
MmeI TCCRAC 4 cut(s) 17, 23, 923, 1061
Mph1103I ATGCAT 3 cut(s) 784, 807, 1775
MroXI GAANNNNTTC 1 cut(s) 1014
MseI TTAA 7 cut(s) 86, 1125, 1194, 1469, 1533, 1638, 2049
MslI CAYNNNNRTG 4 cut(s) 507, 534, 1116, 1772
MspA1I CMGCKG 1 cut(s) 2182
MspCI CTTAAG 1 cut(s) 2048
MspI CCGG 4 cut(s) 417, 468, 1792, 2303
MspR9I CCNGG 5 cut(s) 1792, 1793, 2061, 2303, 2304
MunI CAATTG 2 cut(s) 1322, 1425
Mva1269I GAATGC 5 cut(s) 156, 649, 1059, 1561, 1839
MvaI CCWGG 1 cut(s) 2061
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 5 cut(s) 644, 653, 1127, 1295, 1874
NciI CCSGG 4 cut(s) 1792, 1793, 2303, 2304
NcoI CCATGG 2 cut(s) 610, 2095
NdeI CATATG 3 cut(s) 964, 1620, 1769
NlaIV GGNNCC 4 cut(s) 341, 373, 374, 1405
NmuCI GTSAC 2 cut(s) 974, 1651
NsbI TGCGCA 1 cut(s) 1075
NsiI ATGCAT 3 cut(s) 784, 807, 1775
NspI RCATGY 7 cut(s) 297, 409, 512, 619, 1418, 1480, 1884
PaeI GCATGC 5 cut(s) 297, 409, 512, 619, 1884
PagI TCATGA 1 cut(s) 2143
PceI AGGCCT 1 cut(s) 2046
PciI ACATGT 1 cut(s) 1476
PctI GAATGC 5 cut(s) 156, 649, 1059, 1561, 1839
PdmI GAANNNNTTC 1 cut(s) 1014
PfeI GAWTC 5 cut(s) 906, 1212, 2025, 2140, 2244
PfoI TCCNGGA 1 cut(s) 2059
PkrI GCNGC 7 cut(s) 299, 714, 1390, 1682, 1879, 2014, 2359
PleI GAGTC 9 cut(s) 42, 48, 54, 971, 1573, 1737, 1895, 2064, 2421
PpsI GAGTC 9 cut(s) 42, 48, 54, 971, 1573, 1737, 1895, 2064, 2421
PscI ACATGT 1 cut(s) 1476
PshBI ATTAAT 1 cut(s) 1194
Psp6I CCWGG 1 cut(s) 2059
PspFI CCCAGC 3 cut(s) 656, 1130, 2182
PspGI CCWGG 1 cut(s) 2059
PspN4I GGNNCC 4 cut(s) 341, 373, 374, 1405
PspOMI GGGCCC 1 cut(s) 371
PspPI GGNCC 5 cut(s) 371, 372, 1404, 1966, 2038
PsuI RGATCY 1 cut(s) 498
PvuII CAGCTG 1 cut(s) 2182
RsaI GTAC 8 cut(s) 266, 398, 623, 693, 853, 1444, 1481, 1954
RsaNI GTAC 8 cut(s) 265, 397, 622, 692, 852, 1443, 1480, 1953
RseI CAYNNNNRTG 4 cut(s) 507, 534, 1116, 1772
SaqAI TTAA 7 cut(s) 86, 1125, 1194, 1469, 1533, 1638, 2049
SatI GCNGC 7 cut(s) 298, 713, 1389, 1681, 1878, 2013, 2358
Sau96I GGNCC 5 cut(s) 371, 372, 1404, 1966, 2038
SchI GAGTC 9 cut(s) 43, 49, 55, 971, 1573, 1738, 1896, 2065, 2422
ScrFI CCNGG 5 cut(s) 1792, 1793, 2061, 2303, 2304
SduI GDGCHC 3 cut(s) 375, 1870, 2303
SfaNI GCATC 4 cut(s) 904, 1192, 1999, 2054
SfcI CTRYAG 1 cut(s) 1394
SinI GGWCC 2 cut(s) 1966, 2038
SmaI CCCGGG 2 cut(s) 1793, 2304
SmiMI CAYNNNNRTG 4 cut(s) 507, 534, 1116, 1772
SmlI CTYRAG 3 cut(s) 177, 455, 2048
SmoI CTYRAG 3 cut(s) 177, 455, 2048
SpeI ACTAGT 1 cut(s) 2164
SphI GCATGC 5 cut(s) 297, 409, 512, 619, 1884
SrfI GCCCGGGC 2 cut(s) 1793, 2304
SseBI AGGCCT 1 cut(s) 2046
SsiI CCGC 4 cut(s) 298, 719, 1389, 2335
SspMI CTAG 3 cut(s) 2153, 2165, 2270
StuI AGGCCT 1 cut(s) 2046
StyD4I CCNGG 5 cut(s) 1790, 1791, 2059, 2301, 2302
StyI CCWWGG 4 cut(s) 610, 1588, 2041, 2095
TaiI ACGT 1 cut(s) 546
TaqI TCGA 9 cut(s) 101, 123, 147, 160, 306, 390, 483, 561, 2082
TatI WGTACW 6 cut(s) 264, 396, 621, 691, 1442, 1479
TauI GCSGC 2 cut(s) 300, 1391
TfiI GAWTC 5 cut(s) 906, 1212, 2025, 2140, 2244
Tru1I TTAA 7 cut(s) 86, 1125, 1194, 1469, 1533, 1638, 2049
Tru9I TTAA 7 cut(s) 86, 1125, 1194, 1469, 1533, 1638, 2049
TscAI CASTG 8 cut(s) 556, 694, 769, 890, 1718, 1875, 1993, 2266
TseFI GTSAC 2 cut(s) 974, 1651
TseI GCWGC 5 cut(s) 712, 1680, 1877, 2012, 2357
Tsp45I GTSAC 2 cut(s) 974, 1651
TspGWI ACGGA 1 cut(s) 1449
TspMI CCCGGG 2 cut(s) 1791, 2302
TspRI CASTG 8 cut(s) 556, 694, 769, 890, 1718, 1875, 1993, 2266
Vha464I CTTAAG 1 cut(s) 2048
VneI GTGCAC 1 cut(s) 1866
VpaK11BI GGWCC 2 cut(s) 1966, 2038
VspI ATTAAT 1 cut(s) 1194
XapI RAATTY 6 cut(s) 312, 922, 1929, 1960, 2329, 2439
XceI RCATGY 7 cut(s) 297, 409, 512, 619, 1418, 1480, 1884
XcmI CCANNNNNNNNNTGG 1 cut(s) 2067
XmaI CCCGGG 2 cut(s) 1791, 2302
XmiI GTMKAC 1 cut(s) 540
XmnI GAANNNNTTC 1 cut(s) 1014
XspI CTAG 3 cut(s) 2153, 2165, 2270
Zsp2I ATGCAT 3 cut(s) 784, 807, 1775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.